Supplementary file 2. Phenotype characterization of the different lymphocytes subpopulations and NK subsets analysed in this study.
Cell
subpopulation Cellular phenotype (antigens)
Specific fluorochrome-labelled monoclonal antibodies used
for antigen detection
Company manufacturing the antibodies T-lymphocytes CD3+
CD56-
CD3-PE-Cy7
CD56-APC Biolegend, Clone UCHT1 (
San Diego, CA, USA
) Becton Dickinson (Franklin Lakes, NJ, USA) B-lymphocytes CD19+CD20+
CD19-PE CD20-PE
Beckman Coulter (Fullerton, CA, USA) Becton Dickinson (Franklin Lakes, NJ, USA)
NK CD3-
CD56+
CD3-PE-Cy7
CD56-APC Biolegend, Clone UCHT1 (
San Diego, CA, USA
) Becton Dickinson (Franklin Lakes, NJ, USA)NKT CD3+
CD56+
CD3-PE-Cy7
CD56-APC Biolegend, Clone UCHT1 (
San Diego, CA, USA
) Becton Dickinson (Franklin Lakes, NJ, USA)NKDim CD3-
CD56 dim CD16 bright
CD16-APC-Cy7 CD3-PE-Cy7 CD56-APC
Becton Dickinson (Franklin Lakes, NJ, USA) Biolegend, Clone UCHT1 (
San Diego, CA, USA
) Becton Dickinson (Franklin Lakes, NJ, USA)NKBright CD3-
CD56 bright CD16 dim
CD16-APC-Cy7 CD3-PE-Cy7 CD56-APC
Becton Dickinson (Franklin Lakes, NJ, USA) Biolegend, Clone UCHT1 (
San Diego, CA, USA
) Becton Dickinson (Franklin Lakes, NJ, USA) NK KIR2DL1 CD158a/h+CD158b- CD158e- CD3- CD56+
CD94+
CD158a/h-PE CD158b-FITC CD158e-FITC CD3-PE-Cy7 CD56-APC CD94-FITC
Miltenyi Biotec (Bergisch Gladbach, Germany) BD-PharmingenTM
Miltenyi Biotec (Bergisch Gladbach, Germany) Biolegend, Clone UCHT1 (
San Diego, CA, USA
) BD-PharmingenTMBiolegend, Clone DX22 (
San Diego, CA, USA
)Cell Specific fluorochrome-labelled Company manufacturing the antibodies
subpopulation Cellular phenotype (antigens) monoclonal antibodies used for antigen detection NK KIR2DL2/L3 CD158a/h-
CD158b+
CD158e- CD3- CD56+
CD94+
CD158a/h-PE CD158b-FITC CD158e-PE CD3-PE-Cy7 CD56-APC NKG2A-PE
Miltenyi Biotec (Bergisch Gladbach, Germany) BD-PharmingenTM
Miltenyi Biotec (Bergisch Gladbach, Germany) Biolegend, Clone UCHT1 (
San Diego, CA, USA
) BD-PharmingenTMR&D Systems, Inc.
(
Minneapolis, USA) NK KIR3DL1 CD158a and CD158h-CD158b- CD158e+
CD3- CD56+
CD94+
CD158a/h-FITC CD158b-FITC CD158e-PE CD3-PE-Cy7 CD56-APC CD94-FITC
Miltenyi Biotec (Bergisch Gladbach, Germany) BD-PharmingenTM
Miltenyi Biotec (Bergisch Gladbach, Germany) Biolegend, Clone UCHT1 (
San Diego, CA, USA
) BD-PharmingenTMBiolegend, Clone DX22 (
San Diego, CA, USA
)NK 25 CD25+ CD25-PE Beckman Coulter (Fullerton, CA, USA)
NK 69 CD69+ CD69-PE Miltenyi Biotec (Bergisch Gladbach, Germany)
NK KIR2DS4 CD158i+
CD3- CD56+
CD158i (KIR2DS4)-PE CD3-PE-Cy7
CD56-APC
Miltenyi Biotec (Bergisch Gladbach, Germany) Biolegend, Clone UCHT1 (
San Diego, CA, USA
) BD-PharmingenTMNKG2D CD3-
CD314+
CD56+
CD3-PE-Cy7 CD314-PE CD56-APC
Biolegend, Clone UCHT1 (
San Diego, CA, USA
) Miltenyi Biotec (Bergisch Gladbach, Germany) BD-PharmingenTMNKp44 NKp44+ NKp44-PE Beckman Coulter (Fullerton, CA, USA)
Cell
subpopulation Cellular phenotype (antigens)
Specific fluorochrome-labelled
monoclonal antibodies used Company manufacturing the antibodies
for antigen detection
NKp46 CD335+ CD335-FITC AbD Serotec (BioRad Laboratories Inc.)
NKp30 CD337+ CD337 (NKp30)-PE Miltenyi Biotec (Bergisch Gladbach, Germany)
NK DNAM CD226+
CD3- CD56+
CD226-PE CD3-PE-Cy7 CD56-APC
R&D Systems, Inc.
(
Minneapolis, USA)Biolegend, Clone UCHT1 (
San Diego, CA, USA
) BD-PharmingenTMNKG2A CD3-
CD56+
CD94+
CD3-PE-Cy7 CD56-APC NKG2A-PE
Biolegend, Clone UCHT1 (
San Diego, CA, USA
) BD-PharmingenTMR&D Systems, Inc.
(
Minneapolis, USA)CXCR6 CD3-
CD56+
CXCR6+
CD3-PE-Cy7 CD56-APC CXCR6-PE
Biolegend, Clone UCHT1 (
San Diego, CA, USA
) BD-PharmingenTMR&D Systems, Inc.