Tables SDC 8, Supplementary Table S1

(1)

Tables SDC 8, Supplementary Table S1

# sample # cells

PCR (successfully amplified) ddPCR (copies/µl)

V600E detected V600E f.a. ADO/AI chr 12p chr 5q chr 17p chr 6q EGFR KIT BRAF

1 10 Y Y Y N 890 44800 17 Y 68% Y

2 10 Y N Y N 2840 10256 4.96 Y 8% Y

3 10 Y Y Y N 1502 45600 2.92 Y 11% Y

4 10 Y Y Y Y 2940 24600 2.04 Y 20% Y

5 10 Y N Y N 1226 18880 34.56 Y 0.4% Y

6 5 Y Y Y N 6680 39200 8.56 Y 1.7% Y

7 5 Y Y Y N 76 27720 20.8 N 0% Y

8 5 Y Y Y N 1700 33800 44.4 N 0% Y

9 5 N N N N nd nd nd - - -

10 5 N N N N nd nd nd - - -

11 2 N N N N nd nd nd - - -

12 2 N N N N nd nd nd - - -

13 2 N N N N nd nd nd - - -

14 2 N N N N nd nd nd - - -

15 2 Y Y Y N 444 40000 5.36 Y 5% Y

16 WGA

ctrl Y Y Y N 1880 28280 0.42 Y 28% -

17 DNA

ctrl Y Y Y Y 205.6 190.4 175.2 Y 24.2% -

(2)

Tables SDC 8, Supplementary Table S2

# sample # cells

PCR (successfully amplified) ddPCR (copies/µl)

V600E detected V600E f.a. ADO/AI chr 12p chr 5q chr 17p chr 6q EGFR KIT BRAF

1 1 Y Y Y Y 206 20080 0.54 Y 33% Y

2 1 Y Y Y N 3080 30400 nd - - -

3 1 N N N N nd nd nd - - -

4 1 N N N N nd nd nd - - -

5 1 N N N N nd nd nd - - -

6 1 N N N N nd nd nd - - -

7 1 N N N N nd nd nd - - -

8 1 N N N N nd nd nd - - -

9 1 N N N N nd nd nd - - -

10 1 N N N N nd nd nd - - -

11 1 N N N N nd nd nd - - -

12 1 N N N N nd nd nd - - -

13 1 N N N N nd nd nd - - -

14 1 N N N N nd nd nd - - -

15 1 N N N N nd nd nd - - -

16 1 N N N N nd nd nd - - -

17 1 N N N N nd nd nd - - -

18 1 N N N N nd nd nd - - -

19 1 N N N N nd nd nd - - -

Tables SDC 8: WGA performance assessment. Decreasing numbers of HT29 cells, BRAF V600E heterozygous, were sorted in a 96 well plate: 10, 5, and 2 cells were sorted in 5 different wells (column #1-2, Table S1); 19 single-cell wells were also collected (Table S2).

Tables SDC 8, Supplementary Table S1