Untitled - Dspace Hangtuah

This event was part of the celebration of the 65th anniversary of the faculty as well as the 62nd anniversary of Universitas Gadjah Mada. This event is part of the celebration of the 65th anniversary of the faculty, as well as the 62nd anniversary of Universitas Gadjah Mada.

ANTIOXIDANT AND CYTOTOXIC ACTIVITIES OF ETHANOLIC EXTRACT OF HEDYOTIS CORYMBOSA (L.)

LAM ON BREAST CANCER CELL LINE OF T-47D

Tarwadi 1 , Susi Kusumaningrum 1 and Fery Azis Wijaya 2

Methanolic extracts of Rumput mutiara herbs were prepared with concentrations of 1000 ppm, and 0.0079% DPPH solution (7.9 mg in 100ml methanol). The IC50 value of ethanolic extract of Rumput mutiara (Hedyotis corimbosa) against T-47D cancer cells and normal CHO cells.

DPPH antiradical scavenging activity of Rumput mutiara (Hedyotis corymbosa L.) extract compared with vitamin E and vitamin C: a. DPPH antiradical scavenging activity of Rumput mutiara (Hedyotis corymbosa L.) extract compared with vitamin E and vitamin C: c.

Tabel 2. Proliferative inhibitory effect of ethanolic extract of Rumput mutiara (Hedyotis corimbosa) against T-47D cancer cells and normal CHO cells

CONCLUSION

In this study, the highest concentration of treated extract was 100 ppm, another researcher used higher concentration up to 500 ppm and 1000 ppm. The methanolic extract of this plant at 1000 ppm produced a significant increase in DPPH radical scavenging activity comparable to 1000 ppm of this positive control butylated hydroxyl toluene (BHT) (Sasikumar et al., 2010).

At the end of the test, the activities of rumput mutiara extract are still lower than the two positive controls, vitamin E and vitamin C, even at 100 ppm extract. The result showed that 500 ppm and 200 ppm of Coronet extract and 500 ppm extract showed similar inhibitory effect as 200 ppm α-tocopherol on MDA formation (Amelia, 2006).

MOLECULAR DYNAMIC SIMULATION OF SIRNA AND MODIFIED SIRNAS

The simulations were performed in the NPT ensemble with periodic boundary conditions, a temperature of 300 K and at a pressure of 1 atm. The temperature and pressure were kept constant by Berendsen's weak coupling scheme (Berendsen, 1984) with a coupling parameter of 0.1 ps for temperature, 1.0 ps for pressure and the isothermal compressibility of 4.5∙10-5 bar-1. Time evolution of the occurrence of base pairing in the duplexes is also shown.

Figure 1. Sequences of the duplexes used in this study. The modified residues shown in blue color are derived from naturally occuring siRNA (structure I from PDB 1SI3) (Ma, 2004)

FAST DISSOLVING TABLET FORMULATION OF

METOCLOPRAMIDE HYDROKLORIDE BY ADDITION OF KOLLIDON CL-F AS SUPERSDISINTEGRANT

The diameter of the tablet not more than three times and not less than one third of the thickness of the tablet. Kollidon CL-M proved to be used as a good superdisintergant, as shown by the evaluation results of.

IN VITRO ANTIBACTERIAL ACTIVITY OF NIGELLA SATIVA SEEDS AGAINST STREPTOCOCCUS PYOGENES

This study investigated the antibacterial effect of n-hexane, ethyl acetate and ethanolic seed extract on Streptococcus pyogenes. Zones of inhibition of ethyl acetate extract and ethanol extract in different concentrations against Streptococcus pyogenes.

Figure 1. Bioautography assay of flavonoids (A) and saponin compound (B) in ethyl acetate extract and ethanolic extract

EXTRACTION OF ANTIOXIDANT FROM SHIITAKE MUSHROOM ( LENTINULA EDODES )

AN INITIAL STUDY TO FIND NEW ANTIOXIDANT SOURCE~

The DPPH radical scavenging activity of the extracts was determined by the method of Hu et al. The presence of the antioxidants was proven by the radical scavenging activity of DPPH and the inhibition of linoleic acid oxidation.

Fig. 1 DPPH radical scavenging activity of the extract samples

Furthermore, analog 1, containing an 18-membered polyhydroxylated core and two parts of 3-formamidosalicylyl groups, showed greater anticancer activity than that of the parent antimycin A3 on all cancer cells tested, with IC and 43 µM against HeLa cells, breast MDA- cells. MB-231 cells and prostate PC-3 cells, respectively. Cytotoxicities (IC50, µM) of tetralactone 11, tetraol 3, analogue 1 and antimycin A3 against HeLa cells, breast MDA-MB-231 cells and PC-3 prostate cells.

Figure 2. Structure of Grubbs 1 st , Grubbs 2 nd and Zhan 1B

INTERACTION ANALYSIS OF HEMIN WITH

ANTIMALARIAL ARTEMISININ GROUP THROUGH IN- SILICO AND IN-VITRO APPROACH

Conformation of the active site of hemin in the form of cavities that can be bound by the active components of artemesinin and its derivatives. The change in the absorption peak value of the hemin spectrum after the addition of artemisinin, artesunate and artemether can be seen in Table 2.

Table 1. Docking results

DETERMINATION OF ARTEMISININ IN ETHYL ACETATE FRACTION FROM THE STEMS, ROOTS, LEAVES AND

BY HPLC

HPLC methods can be used for the determination of ethyl acetate fraction of artemisinin in Artemisia vulgaris mutant strains of L. Comparison of levels of artemisinin in methanol and ethyl acetate fraction of methanol extract of Artemisia vulgaris mutant strains of L.

Table V.1. Extraction and Rendemen results

CYTOTOXICITY EFFECT OF ETHANOLIC EXTRACT OF TYPHONIUM FLAGELLIFORME (LODD.) BLUME ON

CHO-T120

The IC-50 values ​​of Typhonium flagelliforme extracts on cancer cells of MCF-7 and normal cells of CHO T120 were 51 and 308 ppm, respectively. This suggests that the ethanolic extract of Typhonium flagelliforme breast cancer cell lines inhibits MCF-7, but not the growth of normal CHO cells.

Figure 1 : Typhonium flagelliforme (A), leaf (B), flower (C) and tuber (D)

SCREENING OF BIOACTIVE COMPOUNDS FROM OLEA EUROPAEA AS GLUTAMINE SYNTHETASE A INHIBITOR

TYPE B THROUGH MOLECULAR DOCKING SIMULATION

Cornoside and demethyloleuropein ligand have more binding site to the domain catalytic than the standard. Among them, cornoside and demethyloleuropein ligand that produced 4 and 6 hydrogen bonds to domain catalytic in glnA.

Table 1. Analysis Docking

FLAVOURING AGENT OPTIMATION OF KALANCHOE PINNATA, PERS. CRUDE EXTRACT LOZENGES

Increasing the concentration of essence and dye, i.e. the color and smell of lozenges Kalanchoe pinnata, Pers. The aroma in the lozenge formulation can be masked by the color and aromatic smell of Kalanchoe pinnata powder extract, Pers., the sour taste of Kalanchoe pinnata powder extract, Pers.

Table 1. the design of dosage formulation of lozenges

EFFECTS OF FERTILIZERS AND DIFFERENT LIGHT INTENSITY ON GROWTH AND ANDROGRAPHOLIDE

Ness)

It was found that when the plants were harvested at 1 month, there was no significant difference in mean fresh weight between those plants treated with different light and fertilizer intensities. The effect of light intensity and fertilizer on mean fresh weight is shown in Fig.

Fig. 1. Average plant height of A. paniculata treated with different light intensity and fertilizer

ACUTE TOXICITY TEST AND LOZENGES TABLET FORMULATION OF KALANCHOE PINNATA P. ETHANOL

EXTRACT

The weight uniformity of 3 lozenge formulas was evaluated and the result is presented in Table 3. The disintegration time test result of 3 lozenge formulations of this research is presented in Table 7.

Table 4. Size uniformity of 3 lozenges tablet formulaes

SIMULTANEOUS DETERMINATION OF CAFFEINE AND

NICOTINAMIDE IN ENERGY DRINKS BY FIRST-ORDER DERIVATIVE SPECTROPHOTOMETRY

Regression analysis data for the determination of caffeine and nicotinamide using first-order derivative spectra. The applicability of the proposed methods for the simultaneous estimation of caffeine and nicotinamide was investigated by testing commercial energy drink powders (brand A and brand B). The results obtained are shown in Table 2.

Fig 1. UV Absorption spectra for caffeine in Fig 2. UV Absorption spectra for nicotinamide water (10 µg/mL) in water (10 µg/mL)

MODULATION OF MACROPHAGE IMMUNE RESPONSES OF EXTRACT MIXTURE OF BETEL LEAF ( PIPER BETLE , L),

HYDROXIDE ON PHAGOCYTIC CELLS OF MICE

Effect of phagocytosis activity and ability of betel, gambier and slaked lime mixture on macrophage cell. A mixture of betel leaves (Piper betle L), gambier (Uncaria gambir, Roxb) and slaked lime (calcium hydroxide) acts as an immunomodulator.

Table 1. Effect phagocitosys activityand capacity of mixture of betle, gambier and slaked lime to macrophage cell

THE TLC PROFILE CHARACTERIZATION OF ETHANOL EXTRACT OF KALANCHOE PINNATA WITH OR WITHOUT

DRYING BY FREEZE AND SPRAY DRYING

Raksasiri, et al

PERIODONTAL POCKET

Raksasiri

Increasing the concentration of carbopol 934 P in the gel formulation significantly decreases the spreading diameter of the gel formulations. The work of syringeability of SMS organogel formulations is less than that of amphiphilogel formulations.

Α-GLUCOSIDASE INHIBITORY AND ANTIOXIDANT ACTIVITIES OF ASPERGILLUS TERREUS MC751

The mode of inhibition of At-M and At-F extracts against α-glucosidase was also investigated by Lineweaver-Burk plot analysis. Antioxidant activity of At-M and At-F extracts measured by the DPPH method and compared with quercetin as a reference.

Figure 1. α-Glucosidase inhibitory effect of At-M and At-F (5-100µg/ml) Quercetin was taken as reference standar

FINGERPRINT STUDY OF FOENICULLUM VULGARE MILL

Chloroform has a low polarity index, and the main component of Foeniculum vulgare Mill. anethole) also has a low polarity index. According to the above results, it can be concluded that the fingerprint/chromatogram profile of ethyl acetate extract of F.vulgare Mill.

Figure 1. Chromatogram profile (λ = 254 nm) on TLC silica plates of n-hexane, chloroform, ethyl acetate, and ethanol extracts after elution with A) n-hexane : ethyl acetate (7 : 3 v/v); B) chloroform : ethyl acetate (9 : 1

STRILIZATION HEAT EFFECT TO GEL BASE PHYSICAL PROPERTIES: ELLING AGENT CMC NA AND CA ALGINATE

CASE STUDY

EFFECT OF STERILIZATION HEAT ON PHYSICAL PROPERTIES OF GEL BASE: CMC NA AND CA ALGINATE RELEASE AGENT. Dry heat sterilization has a greater effect on the viscosity and spreading of the gel base than moist heat sterilization.

Figure 2. Alginate structure.

SAFETY EVALUATION OF URIC ACID-LOWERING NATURAL PRODUCT IN RAT: SUBCHRONIC 16-WEEK

This study aims to conduct subchronic 16-week toxicity test of JAU with blood biochemistry as observed parameters. This experiment aimed to evaluate the sub-chronic effect of JAU at the arranged doses on blood biochemistry in particular.

Tabel a: The average blood biochemistry level of male rats from each group Group

FINGERPRINTS STUDY OF GUAZUMAULMIFOLIA LAMK LEAVES FOR STANDARDIZING TRADITIONAL MEDICINE

Semi-polar extracts (ethyl acetate and chloroform extract) contain semi-polar compounds such as flavonoids, alkaloids and tannins. All extraction spots with their mobile phase can be seen in Figure 1. Homatograms performed with the TLC scanner can be seen in Figures 2 to 5.

Table I. Extracts Weight and Yields

PROTECTIVE EFFECT OF EGCG ON OXIDATIVE DAMAGE IN ENDOTHELIAL PROGENITOR CELLS

Quantification of intracellular ROS level was performed by fluorescence with 2′,7′-dichlorofluorescein diacetate (DCF-DA; Invitrogen) using modified methods of Stolzin & Scutt (2006) and Jie et al. EGCG provides a protective effect against oxidative stress through a variety of mechanisms (Chung, et al., 2003; Guo, et al., 1999).

Figure 1. EPC morphology charac- terization. MNCs (A, 400 X) were cultured on VascGrow TM medium and start to exhibited a spindle-shaped on day 4 (B, 400 X) and more sharp in pattern on day 7 (C, 400X; D, 800X)

COMPARISON ACTIVITIES OF LEAVES AND STEM BARK OF ETHYL ACETATE EXTRACT FROM BAWANG HUTAN

LC50 value of the results BSLT ethyl acetate extract from the bark and leaves of S. The results of the ethyl acetate extracts showed that the ethyl acetate extract from the stem bark of S.

Table 1. The results of extraction of the bark and leaves of S.borneensis with the methanol Extraction process Methanol extract Weight, g (%) color

DISSOLUTION PROFILE OF ACETAMINOPHEN TABLET AND IBUPROFEN TABLET WITH L–HPC 21, L–HPC 22,

WET GRANULATION METHOD

In this experiment, we want to study the effect of L-HPC 21, L-HPC 22 and SSG as comparators on a small hydrophilic substance like Acetaminophen tablets and a hydrophobic substance like Ibuprofen tablets on differentiation dissolution profiles. Indofarma Tbk.), Low Substituted Hydroxy Propyl Cellulose (L–HPC) 21 and 22 Ex Shin-Etsu Chemical Co., Ltd.

Table 1. Composition of tablet excipients in Acetaminophen Tablets

PREPARATION AND CHARACTERIZATION OF PROTEIN- LOADED CHITOSAN NANOPARTICLES WITH ALUMINIUM

HYDROXIDE GEL AS NASAL DELIVERY SYSTEM

The results indicated that there was no significant conformational change in BSA encapsulated in CSNPs with aluminum hydroxide. CD spectra showed no significant conformational change in BSA encapsulated in CSNPs with aluminum hydroxide compared to native BSA.

Figure 2. TEM photograph of a. CSNPs and b., c. CSNPs with aluminium hydroxide gel

REDUCTION OF BLOOD GLUCOSE LEVELS OF ETHANOLIC EXTRACT OF BUNGUR ( LAGERSTROEMIA SPECIOSA [L.]

PERS) LEAVES IN ALLOXAN INDUCED DIABETIC RATS

Percentage of reduction in blood glucose level All groups on the 8th day after the 7th day treatments. Increasing doses of ethanol extract of Bungur leaves increased the effect of blood sugar levels, but not statistically significant by Tukey test at α > 0.05.

METAL CHELATING ACTIVITY OF RICE BRAN AND RICE HUSK

The metal chelating activity of rice husks and rice bran extracts is qualitatively shown in fig. Data on the iron chelating activity of rice bran and rice hulls are shown in Table 1.

Fig. 1. TLC profile of rice husk extract (a), rice bran extract (b), vitamin E (c)* and catechin (d)* on Si gel 60 F254, eluted with n-hexane:ethyl acetate (17:3) under vis (A), uv 254 (B), and sprayed with mixture of 1.68% FeSO 4 and 0.3

LONG TERM EFFECT OF ETHANOLIC EXTRACT OF

Arcman capsule diameter in male and female rats after treatment with ethanolic fenugreek extract for 16 weeks. Renal organ weight in male and female rats after treatment with ethanolic fenugreek extract for 16 weeks.

Table 1. Groups of animal treatment

MOLECULAR DOCKING OF SEVERAL COMPOUNDS OF BUNGUR ( LAGERSTROEMIA SPECIOSA [L.] PERS) LEAVES

TO (IGF-1R) RECEPTOR TYROSINE KINASE WITH MOLEGRO VIRTUAL DOCKER

The secondary structure view of IGF-1R binding five active compounds of Lagerstroemia speciosa [L.] Purple extract, (a) ellagitannin; (b) Penta-O-galloyl-D-glucopyranose (PGG); (c) corosolic acid; (d) laeflorin A; (e) lageflorin B. Hydrophobicity of IGF-1R and five active compounds of Lagerstroemia speciosa [L.] Purple extract, (a) ellagitannin; (b) Penta-O-galloyl-D-glucopyranose (PGG); (c) corosolic acid; (d) laeflorin A; (e) loweflorine B; (green is hydrophobic, orange is hydrophilic).

Figure 1. Chemical structure of (a) ellagitannin; (b) Penta-O-galloyl-glucopyranose (PGG); (c) corosolic acid; (d) lageflorin A; (e) lageflorin B

THE EFFECT OF GIVING TOMATO JUICE ( LYCOPERSICUM ESCULENTUM MILL. ) TO THE AMOUNT OF PLATELETS,

BLEEDING TIME AND COAGULATION TIME OF WHITE MALE RATS OF WISTAR STRAIN

The 0 day

The 7 th day

At day 21, each treatment dose reduced bleeding time, and after being statistically tested, the results did not differ significantly from day 0 (Table 1). On the 21st day, each treatment dose has decreased coagulation time, and after statistical testing, the results did not differ significantly from day 0 (Table 2).

Picture 2. Graph profiles of rats’ coagulation time after being given the test material

DOCKINGSTUDIES RHINACANTHIN DERIVATIVES ASPOTENTIALINHIBITOR OF POLO-LIKE KINASE 1 (Plk1)

Amino acid residues have been selected from the template box in the database menu to draw the two-dimensional structure of the ligands. It indicates that the folded conformation of the ligands were in their most favorable conformations.

Fig 1. Structure of Rhinacanthin M, N, Q and 39 related naphthoquinone esters

INTERACTION BETWEEN BETAMETHASONE-17-VALERATE AND NEOMYCIN SULFATE

6 shows representative SEM images of some of the samples. The morphology of the particles showed prismatic shape of BV and irregular shape of NS. The mixture of BV:NS (1:1), prepared by solvent drop milling, showed an interaction between BV and NS.

Fig. 2. Binary phase diagram of physical mixture of BV and NS

HEPATOPROTECTIVE EFFECT OF WARU ( HIBISCUS TILIACEUS ) LEAVES INFUSION IN PARACETAMOL

INDUCED HEPATOTOXIC RATS

Qualitative testing of the chemical compounds in Hibiscus tiliaceus leaves infused with chemical reagents for flavonoids, polyphenols and saponins (Harborne, 1996). Hibiscus tiliaceus leaves infusion with dose interval 500-1000 mg kg-1 body weight showed the hepatoprotective effect in single dose paracetamol induced hepatotoxic rats.

Table III showed that groups were induced single dose paracetamol orally (2 g kg -1 bodyweight) observed necrosis