Suppl. Fig. 2 Role of NT-3 on cold hyperalgesia and mechanical allodynia induced by constriction of the infraorbital nerve (CION). (A) Facial grooming behavior to cold stimulation of rats was evaluated before (Pre) and on day 4 after CION or Sham surgery before (point B) and at 1 h intervals up to 6 h after local treatment with NT-3 (1 μg/50 µL) or vehicle (50 µL).
Values represent means ± SEM of 9-10 rats (Sham-Vehicle, 10 rats; CION-Vehicle, 9 rats;
CION-NT-3 1 μg, 9 rats).* represent P<0.05 when compared with the Sham group (two-way ANOVA, followed by Tukey test). (B) Mechanical thresholds to the application of von Frey monofilaments of rats was evaluated before (Pre) and on day 15 after CION or Sham surgery before (point B) and at 1 h intervals up to 6 h after local treatment with NT-3 (1 μg/50 µL) or vehicle (50 µL). Values represent means ± SEM of 5-6 rats (Sham-Vehicle, 5 rats;
CION-Vehicle, 6 rats; CION-NT-3 1 μg, 5 rats).* represent P<0.05 when compared with the Sham group (two-way ANOVA, followed by Tukey test). CION, constriction of the infraorbital nerve, ANOVA, analysis of variance; NT-3, neurothrophin-3.
