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BO-10

December - Examination 2019 B.Sc. Pt. III Examination

Molecular Biology and Technology Paper - BO-10

Time : 3 Hours ] [ Max. Marks :- 35

Note: The question paper is divided into three sections A, B and C.

Write answers as per the given instructions.

{ZX}e : ¶h àíZ nÌ "A', "~' Am¡a "g' VrZ IÊS>m| ‘| {d^m{OV h¡& à˶oH$

IÊS> Ho$ {ZX}emZwgma àíZm| Ho$ CÎma Xr{OE&

Section - A 7 × 1 = 7

(Very Short Answer Questions)

Note: Answer all questions. As per the nature of the question delimit your answer in one word, one sentence or maximum up to 30 words. Each question carries 1 mark.

IÊS> - "A' (A{V bKw CÎmar¶ àíZ)

{ZX}e : g^r àíZm| Ho$ CÎma Xr{OE& Amn AnZo CÎma H$mo àíZmZwgma EH$ eãX, EH$ dm³¶ ¶m A{YH$V‘ 30 eãXm| ‘| n[agr{‘V H$s{OE& à˶oH$ àíZ 1 A§H$ H$m h¡&

445

BO-10 / 400 / 4 (1) (P.T.O.)

(2)

445

BO-10 / 400 / 4 (2) (Contd.)

1) (i) Write the two main functions of DNA.

DNA Ho$ Xmo ‘w»¶ H$m¶© {b{I¶o&

(ii) Write the name of purines found in RNA.

RNA ‘| nm¶o OmZo dmbo ß¶yarZ Ho$ Zm‘ {b{I¶o&

(iii) Who proposed the word gene?

OrZ eãX H$mo {H$gZo àñVwV {H$¶m?

(iv) Who proposed double helical DNA model?

DNA H$m {ÛHw$ÊS>br¶ à{Vén {H$gZo àñVwV {H$¶m?

(v) What is plasmid?

ßbmpÁ‘S> ³¶m h¡?

(vi) Write the two application of P.C.R.

P.C.R. Ho$ Xmo AZwà¶moJ {b{I¶o&

(vii) What is inoculation?

g§amonU ³¶m h¡?

Section - B 4 × 3.5 = 14 (Short Answer Questions)

Note: Answer any four questions. Each answer should not exceed 200 words. Each question carries 3.5 marks.

(IÊS> - ~) (bKw CÎmar¶ àíZ)

{ZX}e : {H$Ýht Mma àíZm| Ho$ CÎma Xr{OE& Amn AnZo CÎma H$mo A{YH$V‘

200 eãXm| ‘| n[agr{‘V H$s{OE& à˶oH$ àíZ 3.5 A§H$m| H$m h¡&

(3)

BO-10 / 400 / 4 (3) (P.T.O.) 445

2) Explain the watson and crick model of DNA.

dmQ>gZ Ed§ {H«$H$ Ûmam {X¶ J¶o DNA àmén H$mo g‘PmB¶o&

3) Explain the concept of gene and gene hypothesis.

OrZ {gÕmÝV Ed§ OrZ H$s AdYmaUm H$mo g‘PmB¶o&

4) Write the short note on Genetic Code.

AmZwdm§{eH$ Hy$Q> na g§{jßV {Q>ßnUr {b{I¶o&

5) Define vector ? Describe different vectors used for Gene transfer in plants.

dmhH$ H$mo n[a^m{fV H$[a¶o? nmXnm| ‘| OrZ ñWmZmÝVaU ‘| à¶w³V {d{^ÝZ dmhH$m| Ho$ ~mao ‘o ~VmB¶o&

6) Describe the practical application of tissue culture.

CÎmH$ g§dY©Z Ho$ ì¶dhm[aH$ AZwà¶moJm| H$mo ~VmB¶o&

7) Write a note on Callus culture.

H°$bg g§dY©Z na EH$ {Q>ßnUr {b{IE&

8) Write short notes on.

g§{jßV {Q>ßn{U¶m± {b{I¶o

(i) Laminar air flow / bo{ZZa dm¶w àdmh

(ii) Autoclave / AmQ>mo³bod

9) Write short note on “Importance of Biotechnology”.

""O¡d àmoÚmo{JH$s¨ Ho$ ‘hËd na g§{jßV {Q>ßnUr {b{I¶o&

(4)

445

BO-10 / 400 / 4 (4)

Section - C 2 × 7 = 14 (Long Answer Questions)

Note: Answer any two questions. You have to delimit your each answer maximum up to 500 words. Each question carries 7 marks.

(IÊS> - g) (XrK© CÎmar¶ àíZ)

{ZX}e : {H$Ýht Xmo àíZm| Ho$ CÎma Xr{OE& Amn AnZo CÎma H$mo A{YH$V‘ 500 eãXm| ‘| n[agr{‘V H$aZm h¡& à˶oH$ àíZ 07 A§H$m| H$m h¡&

10) Write short note on the following :

{ZåZ na g§{jßV {Q>ßnUr {b{I¶o …

(i) Restriction enzyme / aoñQ´>r³eZ EÝOmB‘

(ii) Genomic Library / OrZmo{‘H$ bmB~«oar

11) What is culture medium? Mention different techniques of sterlization used in tissue culture.

g§dY©Z ‘mܶ‘ ³¶m hmoVm h¡? CÎmH$ g§dY©Z ‘| à¶w³V hmoZo dmbr {ZO©‘rH$aU VH$ZrH$m| H$m CëboI H$s{OE&

12) Describe the structure of RNA and different types of RNA.

RNA Ho$ {d{^ÝZ àH$ma Ed§ BgH$s g§aMZm H$m dU©Z H$s{OE&

13) Write down the achievements through Biotechnology in crop improvement.

’$gbm| Ho$ gwYma hoVw O¡d àmoÚmo{JH$s H$s CnbpãY¶m± {b{I¶o&

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