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The Indian Agricultural Research Institute, New Delhi, invites applications from MSc and PhD students of IARI for a two weeks Student’s Winter School on “Genomics of plant pathogens and agriculturally important microbes” sponsored by NAHEP-Centre for Advanced Agricultural Science and Technology (CAAST), Indian Council of Agricultural Research, New Delhi.

WHO CAN PARTICIPATE

MSc and PhD students of IARI are eligible to apply. The number of participants will be limited to twenty five ONLY

REGISTRATION FEES: No registration fee is to be paid; the programme is fully sponsored by NAHEP-CAAST

HOW TO APPLY

Complete application form in the prescribed format forwarded by chairperson should reach the Principal investigator, NAHEP-Centre for Advanced Agricultural Science and Technology (CAAST), Division of Plant Physiology, ICAR-IARI, New Delhi on or before 30th October 2018; application form can be downloaded from www.iari.res.in

TRAVEL

No travelling allowance will be provided by the organizers as students are expected to make their own arrangement to reach the venue at 9:30am on all working days

FOOD and ACCOMMODATION

Tea and snacks will be served during the programme and expenditure will be met from the training budget. However, no accommodation will be arranged as students are already accommodated in IARI- hostels

DURATION

Two weeks- December 17-28, 2018

Venue:

Lectures: Auditorium, Division of Plant Pathology, ICAR- Indian Agricultural Research Institute, Pusa Campus, New Delhi-110012.

Practicals: PG laboratory, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, Pusa Campus, New Delhi-110012.

Organizers Course Director Dr. C. Viswanathan

Principal Investigator, NAHEP-Centre for Advanced Agricultural Science and Technology (CAAST) and Head (Acting), Division of Plant Physiology, ICAR-IARI, Pusa

Campus, New Delhi-110012, Email: [email protected] Phone:91-11-25842815, 09013885245

Course Coordinators Dr. A. Kumar

Principal Scientist, Division of Plant Pathology, ICAR-IARI, Pusa Campus,

New Delhi-110012

Email: [email protected], [email protected], Phone: 09540829009

Dr. K. Annapurna Head, Division of Microbiology,

ICAR-IARI, Pusa Campus New Delhi 110 012 Email: [email protected]

Phone: 011-25847649

The programme is jointly coordinated by Divisions of Plant Pathology and Microbiology at IARI

National Agricultural Higher Education Project (NAHEP)

Sponsored Student’s Winter School

December 17 -28, 2018 at

Division of Plant Pathology, ICAR-IARI, Pusa Campus, New Delhi

Organized by

Center for Advanced Agricultural Science and Technology (CAAST)

Indian Agricultural Research Institute Pusa Campus, New Delhi- 110012

Genomics of plant pathogens and

agriculturally important microbes

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About NAHEP-CAAST

Centre for Advanced Agricultural Science and Technology (CAAST) is a new initiative and student centric sub-component of World Bank sponsored National Agricultural Higher Education Project (NAHEP) granted to IARI to provide a platform for strengthening educational and research activities of post graduate and doctoral students.

CAAST theme for IARI is Genomic assisted crop improvement and resource management that specifically aims at inculcating genomics literacy and skills among the student of IARI.

Background

Global food production needs to keep pace with ever growing human population of 7 billion that is expected to touch 10 billion by 2050. With shrinking cultivable area and consequent ‘agricultural habitat loss’ for sustained crop production, one of the approaches for ensuring, sustaining and enhancing the agricultural productivity and nutritional security is by reducing the losses due to biotic and abiotic stress factors. Biotic factors like pest &

diseases, multitude of climate and environmental related abiotic factors are among the major constraints that threaten global agriculture.

Traditionally plant stresses, especially biotic ones are managed by deploying resistant cultivars and application of chemical molecules. These approaches, though very effective, are not universally adopted in all situations.

While crop resistance is not durable, the chemicals are not a sustainable solution as a long term strategy.

Therefore, novel and innovative approaches are, indeed, essential for mitigating the crop losses. Plant associated microorganism are known to play a vital role in shaping and guiding plant growth, development and confers defense against biotic and abiotic stresses.

In the recent years microbe assisted crop production is gaining momentum as a supplementary strategy in agriculture that is expected to make major impact in clean agricultural production. However, the vast diversity of microbial communities in plant associated niches is not exploited properly for want of appropriate technologies.

The cracking of first microbial genome by Craig Venter in the year 1996 has culminated in the birth of science of genomics. In the last two decade, ‘omics science’ and the genomic data has enabled us to understand diverse plant associated microbial communities, pathogens of crop plants and their behavior on plant associated niches. The exponential growth of genome related information and the associated “Omics tools” provided an opportunity for the plant pathologist and microbiologist to understand the population genetics of microorganisms and their host interactions at cellular and genome level.

A total of 312,877 whole genome sequencing projects encompassing most of the plant pathogens and microbes are underway throughout the world that includes 278953 Prokaryotes, 24,987 Eukaryotes, and 8,937 viruses as on 23 August 2018 (https://gold.jgi.doe.gov/). In order to harness the potential of the genome information, we need to create appropriate infrastructure facilities and human resources to face the challenges in the coming decades.

Pathogenomics and microbial genome initiatives at IARI

The ICAR-IARI, New Delhi has made significant contributions in developing crop protection and production technologies for all major crops in India. The institute has core strength in the area of genomics and modern research facilities for conducting advanced genomics programmes.

Recently the institute has published the whole genome sequences of agriculturally important free living nitrogen fixing diazotrophic microorganisms as Pseudomonas stutzeri and Bacillus species, Magnaporthe oryzae inciting blast in rice, Tilletia indica causing Karnal Bunt, Cochliobolus sativus causing spot blotch of wheat, Puccinia striiformis causing yellow rust of wheat, Fusarium fujikuroi inciting bakanae disease of rice, Ralstonia solanacearum causing wilt, Meloidogyne graminicola infecting rice and several plant viruses. Besides, metagenome analysis of plant microbiomes of major crops is also generated and published. With this background the Centre for Advanced Agricultural Science and Technology (CAAST) under NAHEP is organizing 2-weeks Student’s Winter School on “Genomics of plant pathogens and agriculturally important microbes” for the benefit of students of IARI, New Delhi

COURSE OUTLINE

The winter school has the following components:

A. Lectures on principles and practices of basic &

advanced genomic techniques used in Plant Pathology, Microbiology and Nematology

The lectures will be delivered in the forenoon during the training period. Evolution of various pre- and post- genomic era techniques used in plant pathology and microbiology will be discussed.

B. Demonstration and visits for Sequencing and computing facilities

Video demonstrations on genomic library preparation for whole genome sequencing. Visit to facilities like TEM, SEM, Confocal Microscope, Genome sequencers, Bioinformatic data center, Phenomics facility etc.

C. Group activities for case studies

Student groups will be allotted a set of plant pathogens and microorganisms to characterize using genomic tools.

D. Interactive discussion presentation and Quiz Each student is expected to make a short presentation of their present work and future work plan on pathogenomics and microbial genomics programme of their choice organism. Presentation will be facilitated by coordinators during evening hours on all days during the programme. Students are also encouraged to bring their own biological material to work with.

Application deadline: 30th Oct 2018

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Programme

Monday, 17 Dec 2018 09:30-10:00h Registration

10:00-10:10h Welcome address

Head, Division of Plant Pathology 10:10-10:15h About the NAHEP-CAAST programme:

Dr. K. Manjaiah

10:15-10:20h About the SWS programme and ice breaking; student ’ s introduction Dr. Viswanathan C, PI, NAHEP-CAAST 10:20-10:40h Address by Chief Guest-

Dean and Joint Director 10:40-10:45h Vote of thanks

Dr. K. Annapurna 10:45-11:00h Tea

11:00-11:15h Pre-training evaluation A.Kumar and K. Annapurna

11:15-12:15h Lecture 1: Whole genome sequencing of agriculturally important organisms: ICAR initiatives- Dr. R. K. Jain, Dean and Joint Director (Education)

12:15-13:15h Lecture 2: Genome sequencing of plant pathogens and Microbes:

Strategies and Methods Dr. A. Kumar

14:15-17:15h Practical 1 : DNA/RNA isolation from fungi, bacteria and virus Dr. A. Kumar /Dr. Deepa Kamil/Dr. K. Annapurna / Dr. V.

Govindasamy/

Dr. Bikash Mandal

Tuesday, 18 Dec 2018

10:00-11:00h Lecture 3: Single Molecular Real Time Sequencing (SMRT): a revolutionary genome sequencing technology in 21

st

century Mr. Rakshit Chaudhary, PacBio, SpincoBiotech, Chennai 11:00-11:15h Tea

11:30-13:00h Lecture 4: Small genome sequencing: experimental strategies and recent approaches

Dr. Kishore Gaikwad, NRCPB, Delhi

14:15-17:15h Practical 2: PCR Primer designing and validation

A. Kumar, V. Govindasamy and Deepa Kamil, IARI, Delhi Wednesday, 19 Dec 2018

10:00-11:00h Lecture 5: Functional Genomics of agriculturally important microbes

K. Annapurna, IARI, Delhi 11:00-11:15h Tea

11:30-13:00h Lecture 6: Nanopore - Next Generation hassle free Genome Sequencing Dr. Paras Yadav, ILS, Delhi

14:15-17:15h Practical 3: RAW sequence handling and curation, and assembly

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A.Kumar, Deepa Kamil. V. Govindasamy and K. Annapurna, IARI, New Delhi

Thursday, 20 Dec 2018

10:00-11:00h Lecture 6: Functional genomics of fungal pathogens Rashmi Aggarwal, IARI, New Delhi

11:00-11:15h Tea

11:30-13:00h Lecture 7: Genome Assembly: Concepts and updates Dr. Ambika Gaikwad, NBPGR, New Delhi

14:15-17:15h Practical 4: Gene annotation and preparation of data for accessioning

S. Subramanian, V. Govindasamy and A. Kumar, IARI, New Delhi

Friday, 21 Dec 2018

10:00-11:00h Lecture 7: Bioinformatic analyses of whole-genome sequence data DR. Jyothi Malik, Qiagen, Delhi

11:00-11:15h Tea

11:30-13:00h Lecture 8: Methods to decode plant viral genome and their pathogenesis Dr. Supria Chakraborthi, JNU, Delhi

14:15-17:15h Practical 5: Molecular phylogeny,

Dr. Anirban Roy and A. Kumar, IARI, New Delhi

Saturday, 22 Dec 2018 10:00-11:00h Visit to Sequencing facility,

Dr. B. Ramcharan, NRCPB, Delhi 11:00-12:00h Visit to Phenomics facility,

Dr. Viswanathan C.

14:15-16:15h Visit to Bioinformatic facility, Dr. A. R. Rao

Sunday, 23 Dec 2018 Holiday

Monday, 24 Dec 2018

10:00-11:00h Lecture 9: Whole genome based molecular phylogeny Dr. Anirban, IARI, New Delhi

11:00-11:15h Tea

11:30-13:00h Lecture 10: Viral Genomics DR. V. K. Baranwal, IARI, New Delhi 14:15-17:15h Practical 6: RNA isolation for RNA seq

A.Kumar and V. Govindasamy, IARI, New Delhi Tuesday, 25 Dec 2018 Holiday

Wednesday, 26 Dec 2018

10:00-11:00h Lecture 11: Strategies for transcriptome sequencing Viswanathan C., IARI, New Delhi

11:00-11:15h Tea

11:30-13:00h Lecture 12: Databases for microbial and pathogenomics

Dr. Rajiv Kaushik/ Dr. B. Ramakrishnan , IARI, New Delhi

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14:15-17:15h Practical 7: RNA seq data analysis and functional classification of transcripts

DR. Jyothi Malik, Qiagen, Delhi

Thursday, 27 Dec 2018

10:00-11:00h Lecture 13: Gene finding strategies and their validation in silico Dr. Dinesh Kumar, IASRI, New Delhi

11:00-11:15h Tea

11:30-13:00h Lecture 14: Data mining and machine learning tools for whole genome sequencing

Dr. A. R. Rao, IASRI, New Delhi

14:15-17:15h Practical 8: Application of qPCR in microbial and pathogenomics A.Kumar and K. Annapurna, and B. Ramakrishnan, IARI, New Delhi

Friday, 28 Dec 2018

10:00-11:00h Lecture 15: Viral proteins and their interaction with host Dr. Bikash Mandal, IARI, New Delhi

11:00-11:15h Tea

11:30-12:30h Lecture 16: Nematode genomics-an update Dr. Umarao, IARI, New Delhi

12:30 -13:00h Post training evaluation

A. Kumar and K. Annapurna, IARI, New Delhi 14:15-17:15h Valediction and certificate distribution

CAAST-Team

Referensi

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