NARAJOLE RAJ COLLEGE

(1)

NARAJOLE RAJ COLLEGE

(NAAC Accredited ‘B’ Grade Govt.-Aided College) NARAJOLE: PASCHIM MEDINIPUR: PIN-721211

Ph.: 9475429270 e-mail: [email protected]

BOTANY DEPARTMENT SEMESTER IV

PAPER CC8T (Molicular Biology)

(Unit : 1-3) Dr. Uttam Kumar Kanp

A. Short questions each carring 2marks.

1. What is chromatine? What are the chemical components of chromatin?

2. Define Nucleosome. What components are present in a nucleosome?

3. What do you mean by higher order structure of chromatin? Which fraction of the histones playes an important role in building up of this structure?

4. What is heterochromatin? How dose heterochromatin differ from euchromatin?

5. How does B-DAN differ from Z-DNA?

6. What do you mean denaturation and renaturation of DNA?

7. What is mt DNA?

8. What is Okazaki fragments?

9. Why DNA replication is bydirectional?

B. Semi long question each carries 4 or 5 marks.

Difference between:

1. Constitutive heterochromatin and facultative heterochromatin 2. heterochromatin and euchromatin

3. DNA and RNA

4. Conservative and semiconservative replication of DNA

5. Short note on: a. rolling circle, b. θ (theta) mode c. D-loop of replication.

C. Long questions each carrying 6 or 8 marks.

1. Briefly describe the mechanism of DNA replication in prokaryotes /E. coli.

2.

Discuss the salient features of the Watson and Crick’s model of DNA structure with diagram.

3.

Name a major class of RNA. Discuss on their structural aspects and functions.

4. Prof with an experiment that DNA as the carrier of genetic information.

5. Write the function of different Enzymes involved in DNA replication.

(Unit: 4, 5 ) Teacher Name: Professor Nandita Bhakat

A. Each question carries two marks (2×6=12) 1. What is adaptor hypothesis?

2. What is central dogma?

3. What are heat shock proteins?

(2)

NARAJOLE RAJ COLLEGE

4. What is gene silencing?

5. What is lac operon?

6. What is an operon?

B. Each question carries four marks. (4×5=20) 1. Sketch the process of central dogma.

2. Write the features of genetic code.

3. Write the difference between the mode of function of steroids and peptide hormone.

4. What do you mean by wobble hypothesis?

5. Discuss the functions of heat shock proteins in eukaryotic transcription.

C. Each question carries six marks. (6×4=24)

1. Write down the difference in transcription in prokaryotes and eukaryotes.

2. Discuss the prokaryotic transcription in details.

3. Draw and describe the structure and gene regulation of lactose operon.

4. Draw and describe the structure and gene regulation of tryptophan operon.

(Unit: 6,7 ) Teacher Name: Professor Bangamoti Hansda

A. Each question carries two marks. (2×10=20)

1. What is intron?

2. What is exon?

3. What is 5’ capping?

4. What is 3’ poly A tail?

5. What is “Shine Dalgarno Sequence”?

6. What are the stages of Translation?

7. What is the function of Translation?

8. What do you mean by charging of tRNA?

9. What is aminoacyl Trna?

10. What is split gene in biology?

B. Each question carries four marks. (4×6=24)

1. Draw and label the mRNA structure.

2. What do you mean by RNA editing?

3. Write about the process of 5’ capping.

4. Explain the role of aminoacyl tRNA synthatase.

5. Briefly describe the post translational modifications of proteins.

6. What is spliceosome and what its function?

(3)

NARAJOLE RAJ COLLEGE

C. Each question carries six marks. (6×8=48)

1. Briefly describe the RNA processing and its modification.

2. What is the structure and function of ribosome?

3. How does mRNA transport from the nucleus?

4. How is fidelity of protein synthesis maintained?

5. What are the protein inhibitors and how they can inhibit?

6. What happens in initiation of translation?

7. Explain the translation elongation in your own words.

8. Explain the structural aspects of translation termination.