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*Corresponding author: E-mail: [email protected], [email protected];
Microbiology Research Journal International
24(3): 1-11, 2018; Article no.MRJI.42155
ISSN: 2456-7043
(Past name: British Microbiology Research Journal, Past ISSN: 2231-0886, NLM ID: 101608140)
Retracted: Molecular Identification and Antibiotic Sensitivity Pattern of Bacteria Associated with Decomposed Domestic Food Wastes from Akure Metropolis
Oluwabusayo M. Ologun
1*, Bolatito Boboye
1and Oluwole O. Owoyemi
11Department of Microbiology, Federal University of Technology, P.M.B. 704, Akure, Ondo State, Nigeria.
Authors’ contributions This work was carried out in collaboration between all authors. Author OMO designed the study,
performed the statistical analysis, wrote the protocol and wrote the first draft of the manuscript.
Authors OMO and BB managed the analyses of the study. Author OOO managed the literature searches. All authors read and approved the final manuscript.
Article Information
DOI: 10.9734/MRJI/2018/42155 Editor(s):
(1)Dr. Giuseppe Blaiotta, Professor, Department of Agriculture, Division of “Grape and Wine Sciences”, University of Naples Federico II, Via Universita' 100 – Palazzo Mascabruno 80055 Portici, Italy.
Reviewers:
(1)Ruhul Kuddus, Utah Valley University, USA.
(2)Maciej Tadeusz Strzemski, Medical University of Lublin, Poland.
Complete Peer review History:http://www.sciencedomain.org/review-history/25300
Received 17th April 2018 Accepted 24th June 2018 Published 27th June 2018
ABSTRACT
Aim: This research was designed to assess the molecular identities and antibiotic sensitivity pattern of the bacteria isolated from decomposed domestic food wastes in Akure metropolis.
Methodology: Fifteen bacteria were obtained from the Department of Microbiology, Federal University of Technology Akure. The DNA molecules of the bacterial isolates were extracted using bacterial DNA Mini-Prep Kit. The DNA extracted was amplified and sequenced using universal bacterial primers and ABI Prism DNA sequencer respectively prior their nucleotides blast. The antibiotic sensitivity test of the bacterial isolates was carried out using plate assay.
Results: The identified bacterial isolates including Bacillus megaterium, Clostridium difficile, B.
thuringiensis, B. sphaericus, B. mycoides, B. cereus, Pseudomonas asplenii, Paenibacillus macerans, Lactobacillus jensenii, B. badius, B. licheniformis, B. subtilis and L. delbrueckii retained their original name after the molecular identification with the exception of C. humiferium and B.
Original Research Article
Ologun et al.; MRJI, 24(3): 1-11, 2018; Article no.MRJI.42155
2
pumilus that changed to C. pseudotuberculosis and B. cereus respectively while five bacteria showed no result at the sequencing stage. The molecular techniques revealed the strain name of the bacteria. L. delbrueckii was resistant to amoxicillin, chloramphenicol, cefriazone while susceptible to erythromycin with a zone of inhibition (59.77±0.66 mm). C. difficile was susceptible to gentamycin with a zone of inhibition (13.93±0.47 mm).
Conclusion: From this study, the bacterial isolates (C. pseudotuberculosis, B. cereus, B.
licheniformis, B. subtilis, and B. cereus) demonstrated multidrug resistant property. Therefore, this could constitute a serious health threat to the people living in the environment where the wastes are dumped indiscriminately.
Keywords: Bacteria isolates; decomposed; domestic; DNA molecules; food wastes; molecular identity.