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Kharagpur
Chiranjit Chowdhury
Personal Details:
Date of Birth: October, 10, 1982 Nationality: Indian
Father’s Name: Mr. Shyamalendu Bikas Chowdhury
Educational Qualification:
1. Ph.D. in Biotechnology from Indian Institute of Technology, Kharagpur in 2009 2. M.Sc. in Biochemistry from Kalyani University, India in 2005
3. B.Sc. in Chemistry (Honors), Physics, and Mathematics from Kalyani University, India in 2003
4. Higher Secondary Examination (10+2) from West Bengal Council of Higher Secondary Education in 2000 with 77.9% marks
5. Secondary Examination (class 10) from West Bengal Board of Secondary Education in 1998 with 82.5% marks
Work Address:
Department of Biotechnology,
Indian Institute of Technology, Kharagpur Kharagpur - 721302,
West Bengal, INDIA E-ma Phone: +91-9434532893
Home Address :
31, Churaman Chowdhury Lane, PO: Berhampore, Dist. Murshidabad, PIN: 742101
West Bengal, INDIA
Phone - +91-3482-263267 (Home)
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Ph.D. Research Topic:
Understanding the behaviors of soluble penicillin-binding proteins 5 and 6 in Escherichia coli
Awards & Achievements
1. Senior Research Fellow, Department of Science and Technology (DST), Government of India.
2. Institute Research Fellow, Indian Institute of Technology, Kharagpur from January 2006-August 2007.
3. Qualified Graduate Aptitude Test in Engineering (GATE) with Percentile score:
99.42 and All India Rank 39 in the year, 2005.
Laboratory Skills/Techniques Known:
Molecular biology Techniques: Bacterial genomic DNA isolation, Plasmid isolation and purification, DNA extraction and purification from agarose gel, Quantification & purity checking of DNA, Amplification of DNA by PCR, Recombinant plasmid construction (both cloning and expression vector), Preparation of competent cell, DNA
Transformation, Restriction Digestion, Agarose gel electrophoresis, Site directed mutagenesis, Sequence analysis, Protein expression, Bacterial membrane preparation, SDS polyacrylamide gel electrophoresis, Western Blotting.
Separation and Purification Techniques: Ammonium sulfate precipitation, Acetone precipitation, Affinity chromatography, Gel filtration chromatography, Dialysis, Membrane filtration, TLC, RP HPLC
Biochemical Techniques: Qualitative and quantitative analysis of bio-macromolecules (carbohydrate, proteins, lipids), Assay of enzyme (determination of optimum
pH/temperature/activity), Determination of kinetic parameters (Km, kcat, Ki, Kd), determination of IC50, acylation and deacylation rate etc.
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Biophysical Techniques: Circular dichroism and secondary structure determination of protein, UV-Visible and Fluorescence spectroscopy, Fourier transformed infrared spectroscopy.
Microbial Techniques: Handling of microbial culture (solid and liquid), Culture maintenance (cryopreservation), Methods of Sterilization.
Bio-informatics: BLAST, CLUSTALW, Molecular modeling, Docking analysis, Analysis of protein-ligand interactions.
Computation: Operating system: Windows (XP and Vista), Linux (Fedora core)
MS Office (MS Word, MS Excel, MS Power point), Experience in working with Adobe Photoshop 7.0, Analyses of various experimental data through Graphpad Prism, Origin, and Sigma plot softwares, Knowledge in various bio-informatics software like BLAST, CLUSTALW, Jalview, K2d, MODELLER, Autodock, Pymol, Rasmol, Discovery Studio etc, Experience in using various bio-informatics tool like Expasy proteomics server, Psipred, PHD predictions, NCBI BLAST search, DALI server, ORF finder etc.
Knowledge in various softwares like Bioedit, Chromas and Jellyfish etc., for sequence analyses.
Papers published /communicated in peer reviewed journals:
Anindya S. Ghosh, Chiranjit Chowdhury, David E Nelson. Physiological functions of D- alanine carboxypeptidases in Escherichia coli (2008). Trends Microbiol. 16, 309–
317.
Chiranjit Chowdhury, Tapas R. Nayak, Kevin D. Young and Anindya S. Ghosh. A weak DD-carboxypeptidase activity explains the inability of PBP 6 to substitute for PBP 5 in maintaining normal cell shape in E. coli (2009). FEMS Microbiol. Lett. 303, 76–83.
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Sujoy K. Sarkar, Chiranjit Chowdhury and Anindya S. Ghosh. Deletion of PBP 5 sensitizes Escherichia coli cells to beta-lactam agents (2010). Int. J. Antimicrob.
Agents 35, 244–249.
Papers communicated in peer reviewed journals
Change in the active site micro-architecture alters the in vivo function of PBP 5 and 6 in Escherichia coli by Chiranjit Chowdhury and Anindya S. Ghosh
Differential behavior of PBP 5 and 6 in exerting intrinsic beta-lactam resistance in Escherichia coli by Chiranjit Chowdhury, Sujoy K. Sarkar and Anindya S. Ghosh
Poster presented-
Sarkar, S. K., Chowdhury, C., Ghosh, A. S., 2008. PBP 5 acts as an intrinsic modulator of beta-lactam sensitivity in Escherichia coli. International symposium on
“Integrating Physics, Chemistry, Mathematics and Biology to understand living system”, held at Bose Institute, Kolkata, December 4–6, 2008
Attended several both national and international workshops and symposia
a) National workshop on Protein folding and Bio-informatics organized by Bose Institute, Kolkata during 4th and 5th March, 2008
b) Assisted in the hands on training of the National workshop on Bioinformatics in Genomics and Proteomics organized by Department of Biotechnology, IIT Kharagpur and Department of Biotechnology, Govt. of India during October 09- 10, 2009
c) Workshop on Flow Cytometry and its Research Application organized by Department of Biotechnology, IIT Kharagpur in association with BD Biosciences, India during December 18-19, 2006
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References
1. Dr. Anindya Sundar Ghosh Assistant Professor
Department of Biotechnology
Indian Institute of Technology Kharagpur Kharagpur, West Bengal, India
Phone No. +91 - 3222 - 283798
Email address
2. Prof. Ananta K. Ghosh Professor
Department of Biotechnology
Indian Institute of Technology Kharagpur Kharagpur, West Bengal, India
Phone No. +91 - 3222 – 283762
Email address
3. Prof. Amit K. Das Professor
Department of Biotechnology
Indian Institute of Technology Kharagpur Kharagpur, West Bengal, India
Phone No. +91 - 3222 – 283756
Email address
