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تشخیص مولکولی گونه‌های Mycosphaerella ایجادکننده بیماری مرکب سیگاتوکا با استفاده از آغازگرهای اختصاصی

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Membagikan "تشخیص مولکولی گونه‌های Mycosphaerella ایجادکننده بیماری مرکب سیگاتوکا با استفاده از آغازگرهای اختصاصی"

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E-mail: arzanlou@ hotmail.com

ﺨﺸﺗ ﻴ ﻟﻮﻜﻟﻮﻣ ﺺ ﻲ

ﻪﻧﻮﮔ ﺎﻫ ي

Mycosphaerella

ا ﻳ ﺑ هﺪﻨﻨﻛدﺎﺠ ﻴ

رﺎﻤ ي

ﻲ ﺻﺎﺼﺘﺧا ي ﺎﻫﺮﮔزﺎﻏآ زا هدﺎﻔﺘﺳا ﺎﺑ ﺎﻛﻮﺗﺎﮕ ﻴ ﺳ ﺐﻛﺮﻣ

ﺪﻬﻣ ي ﻮﻠﻧزرا

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X845 Mycosphaerella fijiensis

X848 Mycosphaerella fijiensis

X842 Mycosphaerella fijiensis

X841 Mycosphaerella fijiensis

!

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CIRAD89 Mycosphaerella fijiensis

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X858 Mycosphaerella musicola

X862 Mycosphaerella musicola

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X856 Mycosphaerella musicola

X954 Mycosphaerella musicola

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& #

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CIRAD670 Mycosphaerella eumusae

+

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,-

X1023 Mycosphaerella lateralis

& #

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CBS 151.34 Cordana musae

( )

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CBS 161.74 Metulocladosporiella musae

( )

CBS 113862 Metulocladosporiella musicola

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L) 4 ( )

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M'' K7

N&8 " + O%<3DNA

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M. fijiensis M. musicala

M. eumusae

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) ! K "! !#H (

M. fijiensis

L) 4 P ( )

5 6

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QK ) K "! !#H (

!

M. musicola

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M. eumusae

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) DNA kb plus

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I

@ /

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(MMactF2/ MMactRb)

. L) 4 ( )

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( )

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M. fijiensis M. musicola

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8 9

" + O%<3DNA

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M. eumusae

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10 DNA

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)DNA kb plus

- ( M=

200 bp 500 bp

(8)

I T / !"H J <

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.( ACTF/ MEactR)

L) 4 ) (

1 4

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M. fijiensis

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! K "!

M. musicola

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M. eumusae

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Johanson et al., 1994; Crous & Mourichon 2002;

.

Marin et al., 2003; Arzanlou et al., 2007a, 2009)

.

, B

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L Y

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1

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M 1 2 3 4 5

M 1 2 3 4 5 M 1 2 3 4 5

500 bp

1 2 3

630 bp

(9)

+ 0 1 /

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v#

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P2

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+ '4 (#

Mycosphaerella

1! + ( 4

"

! !

/01

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.(

Arzanlou et al.

(2007a)

ATV +I,1% 1 +4)

t$ P<.

"

1 }

8%

"

P 9 +#

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M. musicola

7

M. fijiensis

1

M. eumusae

! a>O M + (

)

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( F+J =!0 % '+%

hO

"

3 + ‡' + '4DNA

(#

! v#

P2

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>)

"

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# ( t$

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1 +I

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E1. ! d

1!

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U.

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1F % ! WX

L, ! '4

, ) 7' ', [

(Arzanlou et al., 2007a,b,

2008, 2009)

T' %

"

[%1 9 +"9'

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. + hO O

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"

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Arzanlou et al., 2007a, 2008, 2009)

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.

et al., 2007a, 2009)

.

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8

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#+4) , F+J ATV (

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+ '

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, .

)

*

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+

#+4) ( ATV F+J ! ,

[#1xR

+ ( '4

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REFERENCES

1. Arzanlou, M., Abeln, E. C. A., Kema, G. H. J., Waalwijk, C., Carlier, J., Vries, I de, Guzmán, M. &

Crous, P. W. (2007a). Molecular diagnostics for the Sigatoka disease complex of banana.

Phytopathology, 97, 1112-1118.

2. Arzanlou, M., Groenewald, J. Z., Gams, W., Braun, U., Shin, H. D. & Crous, P. W. (2007b).

(10)

Phylogenetic and morphotaxonomic revision of Ramichloridium and allied genera. Studies in Mycology, 58, 57-93.

3. Arzanlou, M., Groenewald, J. Z., Fullerton, R. A., Abeln, E. C. A., Carlier, J., Zapater, M-F., Buddenhagen, I. W., Viljoen A. & Crous, P. W. (2008). Multiple gene genealogies and phenotypic characters differentiate several novel species of Mycosphaerella and related anamorphs on banana.

Persoonia, 20, 19-37.

4. Arzanlou, M., Kema, G. H. J., Waalwijk, C., Carlier, J., Vries, I. de, Guzmán, M., Vargas, M. A., Helder, J. & Crous, P. W. (2009). Molecular diagnostics for the Sigatoka disease complex and Radopholus similis in banana. Acta Horticulture, 828, 237-234.

5. Balint-Kurti, P. J., May, G. D. & Churchill, A. C. L. (2001). Development of a transformation system for Mycosphaerella pathogens of banana: a tool for the study of host/pathogen interactions. FEMS Microbiology Letters, 195, 9-15.

6. Carlier, J., Zapater, M. F., Lapeyre, F., Jones, D. R. & Mourichon, X. (2000). Septoria leaf spot of banana: a newly discovered disease caused by Mycosphaerella eumusae (anamorph Septoria eumusae).

Phytopathology, 90, 884-890.

7. Crous, P. W. (1998). Mycosphaerella spp. and their anamorphs associated with leaf spot diseases of Eucalyptus. Mycological Memories, 21, 1-170.

8. Crous, P. W., Groenewald, J. Z., Pongpanich, K., Himaman, W., Arzanlou, M. & Wingfield, M. J.

(2004). Cryptic speciation and host specificity among Mycosphaerella spp. occurring on Australian Acacia species grown as exotics in the tropics. Studies in Mycology, 50, 457-469.

9. Crous, P. W. & Groenewald, J. Z. (2005). Hosts, species and genotypes: opinions versus data.

Australasian Plant Pathology, 34, 463-470.

10. Crous, P. W. & Mourichon, X. (2002). Mycosphaerella eumusae and its anamorph Pseudocercospora eumusae spp. nov.: causal agent of eumusae leaf spot disease of banana. Sydowia, 54, 35-43.

11. Johanson, A. & Jeger, M. J. (1993). Use of PCR for detection of Mycosphaerella fijiensis and M.

musicola, the causal agents of sigatoka leaf spot in banana and plantain. Mycological Research, 97, 670-674.

12. Johanson, A., Crowhurst, R. N., Rikkerink, E. H. A., Fullerton R. A. & Templeton, M. D. (1994). The use of species-specific DNA probes for the identification of Mycosphaerella fijiensis and M. musicola, the causal agents of Sigatoka disease of banana. Plant Pathology, 43, 701-707.

13. Lievens, B. & Thomma, B. P. H. J. (2005). Recent developments in pathogen detection arrays:

implications for fungal plant pathogens and use in practice. Phytopathology, 95, 1374-1380.

14. Marin, D. H., Romero, R. A., Guzman M. & Sutton, T. B. (2003). Black Sigatoka an increasing threat to banana cultivation. Plant Disease, 87, 208-222.

15. Maxwell, A., Jackson, S. L., Dell B. & Hardy, G. E. S. J. (2005). PCR-identification of Mycosphaerella species associated with leaf diseases of Eucalyptus. Mycological Research, 109, 992-1004.

16. Ploetz, R. (2000). Black Sigatoka. Pesticide Outlook, 11, 19-23.

Referensi

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