کربوهیدرازهای گوارشی در لارو کرم خراط Zeuzera pyrina (Lep.: Cossidae)

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: 09354509815 E-mail: vnaveh@ut.ac.ir

Zeuzera pyrina (Lep.: Cossidae)

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1935; Carter 1984; Gatwick 1992)

>3 B 0 4/ .

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Kutinkova et al., 2006)

* @ = )" 4 &'( 3.

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4<

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al., 2000) .

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. 3

F E Q A + P V E (

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Y3 A *47 4f 9c D "/ )-0 < 9 J A # , 74#

7 E Q # 7 3 A

(Janecek S., 1997; MacGregor

EA et al., 2001)

V E ( 3 H E Y34< .

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&03 @ A -

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&]0 93 3 DQ gQ * 9 <^

-

Q *

47 3 9 g

.(Terra and Ferreira, 1994)

)*

!Q( >3 "U3 V 3> E 3 h 93 P -

>e (

Drosophila melanogaster (Doane,1969)

#34< V E ( 3 >3 Q DQ 64' ` 8 # +* S

Callosobruchus chinensis 9 1

(Podoler and

Applebaum, 1971)

<

Tenebrio molitor

\0 #

(Buonocore et al., 1976) Bombyx mori

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Y LQ T

(Kanekatsu, 1978) Zabrotes

subfasciatus

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(Campos et al., 1989)

/ i 3E

&03 . )LQ T 3

!Q( Y U X5 @ -

>e (

Sitophilus zeamais Sitophilus granarius

E

&03 )-'4/ 34R 0 4*

(Baker, 1983) . 47 A <

9 )7 &03

& Q L' 4"7 @ A ? 3E'3 3 V E ( 3

A

Baker .

64' E 3 3 L# J A 3 L' @ A

3 V E (

S. zeamais@34*

P

S. granarius@34*

64' ` A <

47

&03 . 0 4*

6 C 3 @ A

Sitophilus oryzae@

Tribolium castaneum

H

!Q( >3 V > E 3 -

3 9 < 3 >e ( .(Chen et al.,

1992)

V E ( -5 0 ) , Y3 H >3 @ * A

&03 VA . )*

93 P h

X)L 3 9 <

3 -5 0

!Q(

-

>e (

@4-7 *

Alteromonas haloplanctis

) , *

93 3 - F

.(Aghajari et al., 1998) *

* 54

jQ 5 >3 7 # 3E/ 3 )* E V E ( 3 @> 0

V<1

(Lemos et al., 1990; Terra and 5

Ferreira, 1994)

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-

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jQ 5 5

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&03

(MacGregor, 1993)

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>e (

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&Chrispeels MJ 1997; Strobl S et al., 1998) .

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>e (

`0 0 54* >3 H34]-03 E ( XP )LQ T A

&03

.(Applebaum et al., 1965)

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F E Q A + P E >3 > 7

1,4-3- @ A

glucosidic

E Q # 7 3 7

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& R

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(3)

E/ R3E* ^

&03 i 3 .

3 >3 )7

Y34<

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melifera

*Apis

) )" 0

(Terra et @

al., 1996; R.J. Baker et al., 1972) Glyphodes

pyloalis

Y LQ T O

(Ghadamyari et al., 2010)

Brachynema germari

0 4* *

(Ramzi and @ A

Hosseininaveh, 2010)

47 3

. -*

-

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A

) EC# VA 7 0 f Q3 @ 9 47

>3 +U @ A

A

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)* .

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C 3 @ > Y LQ T

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)-03 )* 93 3 DQ gQ * @

Thaumetopoea pityocampa

(Lep.:Notodontidae)

\0 # )7

(F. Pratviel-Sosa et

al., 1986) apollo L. (Lep.: Papilionidae)

Parnassius

\0 # E

(M. Nakonieczny et al.,

2006)

47 3

. )-,Q3 @ V E ( 3 0 4* )* 93 #

54*

`0 0 >3

4 o A

(Yapi,a, et al., 2009)

Rhynchophorus palmarum L. (Col.:

Curculionidae)

= <

Rhagium inquisitor (Col.:

Cerambycidae) (Chipoulet et al.,1985)

<

Zabrotes subfasciatus (Col.: Bruchidae) (Silva et

al., 2001) (Ferreira et al., 2001) Tenebrio - 8

molitor (Col.: Tenebrionidae)

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3 .

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1. 4-Nitrophenyl 3-D-glucopyranoside 2. 4-Nitrophenyl F-D-glucopyranoside

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pH@ @ A 3

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9 Y34Z3

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& Q L' 4* = "

V E (

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>3 )7 & Q L' ) 8* pH

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pH L#

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544 / 2

= "

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= "

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3

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& ./

30 9 D# 9 )O ^ >3 )S R V e 9 3

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UV

0 4*

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.

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? >( _ - )7 3 9 < A

V E ( @34* ) 8* pH

!Q(

-

>e ( 8

* ) +D 2 .(

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SE)

±

!Q( ( - 4- / >e ( pH @

@ A 3

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L qe-53 b4-< q4 3 @ 3

(p<0.05)

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10pH

4 > )* & Q L' 93E 40

U

pH 0

11 0 4!U )* & Q L' 3 .

>3 4f q4O 3pH

# 6 4 > )* & Q L' 93E 40

&t03 )t-' ?A 7 U .

d tt- 8

pH 8 3 > 4tt* tt5 >3 3 &tt Q L' 4#= tt*

&03 . 3 Y tLQ T >3 t54* t* )t > t 3 @ A

3 D E &*34R )-'4/ Y U .

th 93 t P )t*

pH

@34t* )t 8*

Hypothenemus hampei

5 t ( &t0 *

.(Valencia-Jimenez et al., 2000)

4f 0 4*

@

@ )7

t 6 tC 3 )t 8* pH

Bt #4# )t* tR3E* ^ @34*

5 6

&t0 *

(Ramzi and Hosseininaveh,2010). t ( t J A

5.4pH

@34tt*

Tribolium castaneum

Y ttLQ T ttO

(Applebaum and Konijn, 1965)

),tt0 G tt

.

ttttLQ T tttt!Q( @ )tttt7 # -

tttt >e tttt (

Callosobruchus chinensis

)" t0 *

(Podoler and @

Applebaum,1971)

6 C 3

&t Q L' B0 pH

2 / 5 ( &0 *

. Y LQ T 54* )14/3 B0 pH

–t!Q(

>e ttt ( ttt54*

Y34<ttt ttt A

Trogoderma

granarium

4- / 6

# 9

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@

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.

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-*

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= i 3 t/ f-t0 t >3

64t7

B0 2345

4#

( &0 * pH

6

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3 3

pH A !Q( >3 ( &0 * -

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@ A>3 tt A *47 4tth73

3 /

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pH h

!Q( @34* V -F3 -

-* >3 > 7 "/

-

>3 > 7 "/

Glyphodes pyloalis L B #4# )*

5 / 7 5 / 5

( &0 *

(Ghadamyari et al., 2010). O )* 3 .

+D )* )H # * "7 3

@ A>3 > 7 "/ & Q L' 4-< *

(7)

= 4- / * 2345 647

pH @ 5

# 8

* .

^ L & Q L' @ )7 @4f 0 4*

Brachynema germari R3E*

3

pH 6 C V -F3

3 R3E* ^ @34* B #4# )* & Q L' 4<

5 / 5 6

.(Ramzi and Hosseininaveh, *

2010)

!Q( & Q L' @ 4-8*

-

!Q( >e ( -

>3 > 7 "/

-*

-

= >3 > 7 "/

@4' ) 3 4F )*

B #4#

40 35 35 ( &0 * )

+D 4 .(

+D 4 -

!Q( @ A V E ( & Q L' @ ) 8* @ 9 j]< Y34Z3 0 4*

-

!Q( >e ( -

-* >3 > 7 "/

-

>3 > 7 "/

G 3 )* )H # *

3

& Q L' V E (

!Q(

-

@ >3 >3 > 7 "/

20

# 60 0 )H r "

)*

>3 ? * 80

0 U

.

@ 4- / 3

-* @34* 8* <

-

@ @ ) 3 >3 > 7 "/

20

#

50 0 )H r "

( &0 * .

>3 4#= * @ 70

)H

0 r "

!Q( & Q L' -

-* >3 > 7 "/

- )* 3 >3 > 7 "/

4 >

50

3 F Q E 4 0 A ?A 7 U

7 .

!Q(

- (

>3 & Q L' ? 3E'3 >e 35

L* )* )H

€ 4 40

0 )H r "

3 S 4-< * )*

de 7 & Q L' ? 3E'3 0 5

&03 8<

.

@4 / >3 3 @ > Y34< 3 3 0 4*

&03 . 3 Y34< 4 0 @ Y LQ T >3 )7 A

0 4*

3 *

@ A

# 3 ( &0 * x SG

3 &8* <

.

!Q( & Q L' ) 8* @ -

>3 > 7 "/

-*

-

>3 > 7 "/

45 r "0 )H

&03 ( &0 *

(Ramzi and

) 8* @ 4f 0 4* .

!Q( & Q L' @34*

- >e (

Hypera postica

35 r "0 )H

&03 ),0 G

(Vatanparast

and Hosseininaveh, 2010)

0 4* .

@ A 6 C 3

Prostephanus truncates @

@34* ) 8* @

!Q( & Q L' -

* >e ( 40

- 30

&0 * r "0 )H (

. 3 F Q E 4 0 V E ( & Q L' ? 3E'3 * 14A

(Mendiola-Olaya et al., 2000) 47

.

4*

9 4Z3 0

3 & Q L' 4* K"-] @ A 3

V E (

3 9 <

) +D 5 .(

'

!Q( & Q L -

4 Z # &G# >e (

9 3 > 4* 3 !"-] Y 0 K"-] @ A

KCl .

CaCl2

V E ( 3 & Q L' ? 3E'3 BH .

4f

9

MgCl2 A 4-< * ) A >3 NaCl

SDS

V E ( & Q L' ?A 7 BH .

!Q(

-

&G# >3 > 7 "/

4 Z #

CaCl2

9( & Q L'

&' ?A 7

SDS . & 8 VA

V E ( & Q L' ?A 7 BH .

)0 )7 @ O )*

&o"^

5 / 1 5 / 2 5 / 3 4* 7 * 4 Z # = "

&o"^ Q & 3 V E ( & Q L' 5

/ 4

= "

)* & Q L' 70

0 U

NaCl .

MgCl2

3 4Z

!Q( & Q L' 4* < 3E'3 -

>3 > 7 "/

3 9 <

.

? 3E'3

&o"^

!Q( & Q L' 4* 3 14Z3 KCl

-

>3 > 7 "/

& 3 . -*

-

&o"^ &G# 3 -*3 >3 > 7 "/

15

= "

>3

CaCl2

& 3 3 & Q L' @ LU .

4 0 3

&o"^

BH &o"^ ? 3E'3 * 9 3 K"-] @ A

& Q L' ?A 7 V E ( 3 @

KCl .

SDS

-* & Q L' ?A 7 BH -

>3 > 7 "/

NaCl . 4 Z #

& 3 V E ( 3 & Q L' 4* 3 1 .

MgCl2 3 E

V E ( 3 & Q L' ? 3E'3 BH @ # € C .

(8)

/ 3 > * 4Z3

>( SDS

9

@ A Y ,Z3 )* K"-]

4Z3 h 93 P )* &03 0

!Q( @ 9 3 -

>e (

0 R3E* ^ H

Brachynema

germari

V E ( 3 & Q L' ?A 7 BH .(Ramzi

and Hosseininaveh,2010)

@ A 9

Cu²⁺

N Mg²⁺

)*

4A & Q L' 4* 4 Z # 6 P ? 3E'3 BH B #4#

!Q( V E ( -

>3 > 7 "/

-*

- 0 >3 > 7 "/

Lygus

hesperus (Zeng and Cohen et al., 2001)

.

l @4f 0 4*

KCl

0 G ? 3E'3 NaCl

!Q( & Q L' -

I4* O4540 >e (

Hypera postica )C B,0 3

.(Vatanparast

and Hosseininaveh, 2010)

@ )7 # LQ T O

Rhynchophorus palmarum

9 &'4/ Y U A

9 J A

CuCl2

ZnCl2

FeCl3

DTNB

4Z3 pCMB

-* @ / 7 8

- 3 )- 3 >3 > 7 "/

. '4O >3

BaCl2

MgCl2

MnCl2

SrCl2

CaCl2

& Q L' 4* @4Z3

- 3 V E ( 3

(Yapi et al., 2009) .

+D 5 - 9 Y34Z3 0 4*

@ A

&o"^ K"-]

!Q( V E ( )0 & Q L' 4* Y !- @ A -

!Q( >e ( -

-* >3 > 7 "/

-

>3 > 7 "/

3 )* )H # * @ A 3 >3 ( &0 * @ A

!Q( V E ( @ 3 F -

>3 > 7 "/

6 pH

4-< *

& ./ >3 L* 3 & Q L' 1

10 3 & 3 &P 0

4- / )7 &03 Q

pH@ A @ 5

# 7 V E ( & Q L'

(9)

&03 0 5 4 S 4-< * )*

) +D 6 .(

-*

-

4- / 3 @ 3 F 4-< * VA >3 > 7 "/

pH@ 4

#

6 3 ) +D 7 (.

3 & Q L' 4-< * )D (

& ./ >3 pF V E ( 1

10

&P 0 6 pH

3 • .

!Q( & Q L' -

O >3 pF >e ( 1

9 >( >3 &P 0

F 4- / @ 3

pH @ 7

# 9

>3 ? * )*

80 U

& ./ >3 L* )D ( 0 10

A &P 0

>3 ? * )* G 10

4-< * &' E U 5 &Q

6pH

)*

80

0 U

) +D 8 .(

!Q( & Q L' 4-< * )* < @ A 0 4*

-

>e (

Hypera postica L*

& ./ >3 1

10

&P 0

5 pH

3 >3 L* 3 • 47 3 F Q E pH

(Vatanparast and Hosseininaveh, 2010)

4-< * .

-* @ 3 F -

>3 > 7 "/

Rhynchophorus

palmarum

4- /

pH @ * 5

# 6 ( &0 *

(Yapi et al., 2009) .

+D 6 - Q( @ 3 F 0 4*

! -

& ./ >3 L* >3 > 7 "/

1 10

&P 0

+D 7 - -* @ 3 F 0 4*

-

& ./ >3 L* >3 > 7 "/

1 10

&P 0

+D 8 -

!Q( @ 3 F 0 4*

-

& ./ >3 L* >e ( 1

10

&P 0

Km 3 S

Vmax

@34* ? 73 3 ),0 G

!Q(

-

>3 > 7 "/

B #4# )*

52 / 3

= "

041 / 0

" 4* )S R 4* 4D -* @34* # 4F 64/

- B #4# )* >3 > 7 "/

24 / 2

"

= 028 / 0

4D

" 4* )S R 4*

- ( &0 * # 4F 64/

.

R 3 Y LQ T >3 +U _ - _ - 54* * &

(10)

0 4* >3 +U )* < @ A

VA 3 5 3 . 93 P )*

Vmax h

!Q(

-

>e (

R3E* ^ >3 +U

B #4# )*

064 / 0 036 / 0

" 4* )S R 4* 4D # 4F 64/

),0 G

&03

Km. V E ( 3 )* 2 *4

R3E* ^

B. germari

B #4# )* E 77

/ 0 41 / 0

= "

( &0 * .

!Q( W X5 3 -

> 7 "/

>3

Vmax

@34*

B #4# )* R3E* ^ 036

/ 0 021 / 0 4D

" 4* )S R 4*

# 4F 64/

Km

B #4# )* +U

23 / 6 07 / 2

"

( &0 * = .

0 4*

-* @ A

-

)* 4 S R3E* ^ E >3 > 7 "/

B #4#

027 / 0 012 / 0 )S R 4* 4D

" 4*

64/

4 S ) < * &P40 @34* # 4F 71

/ 4 35 / 0 )* 3

@34* B #4#

Km

9 < R3E* ^ >3 +U

(Ramzi and Hosseininaveh, 2010) 3

. Km

Vmax

-*

-

>3 > 7 "/

Glyphodes pyloalis

)*

B #4#

99 / 0

"

= 3 / 0

)S R 4* 4D

4*

"

( &0 * # 4F 64/

(Ghadamyari et al.,

2010)

+D .

9

!Q( )* 2 *4 q34/

- n M )* >e (

9 < 3 V E ( >3 64' E 3 H A

.

!Q( & Q L' -

-* >3 > 7 "/

- 4A a * ` O 8 # VA >3 > 7 "/

64' E 3 ` H >3 7 )7 >3 63 7 4A @34* L'

V E ( 3

&0 A .

+D 9 -

!Q(

-

>e ( )

&03 & 0 (

!Q(

-

>3 > 7 "/

) }1 & 0 (

-*

-

>3 > 7 "/

)

\0 (

jQ 5 (4'

-* @> 0 -

>3 > 7 "/

Rhynchophorus palmarum

V E ( 3 >3 * ` 8 #

V- 0

SDS-PAGE

A <

(Yapi et al.,

2009)

.

* 3 L#

A < @ A

!Q( 634/ 3>

-

/ F K"-] 0 R3E* ^ >e (

1

* P

(Ramzi and

64' E 3 ` )D 3 & 8 .

!Q( & Q L' 634/ 3> 0 4* +U -

>e (

)C I4* O4540

Hypera postica

*

(Vatanparast and Hosseininaveh, 2010)

)-' .

@ A

)-'4/ Y U 0 4* Q 3 ?A ‚F 3 >3 +U

* 2345 647 @ ) > 3 .

3 &03 3 9 F

&'( 3 4Z ! 4- 7 &8H _ - 4 / 34R !-03 .

. "

+

` 7 )-] 34# 6 S 9 A / Q #

&'( # 7 -

?7

q4X Y '( ) "P @4- 7 @ A

.

VQ 0 & > \ G d - 8

& 3 V A3 5 E @4#

.

@34*

/ 7 8 ) F 4* Q4- 7 @‚#34-03 ` @ 34R4*

V E ( Y U X5 0 6 R Q 3 ) .N#

-

3 / @ A

.(Strobl et al., 1998) *

* 0 34* *

V E (

+ 5 @ A

V- 0 34O &8H 3 V8' i 3 /

* Q4- 7 @ A

# 4F >3 !-03 V E ( 3 7 8 @ A

< * A ƒ

(11)

= * SQ * O )* A / 4*

. 0 4* 4o )*

@ A

G )7 )* < Y34< 4< 3 @34* ) / 3

* &03 2345 647 8 ( Y 5

&03 6>=

.

A DA3 45( >3 V A3 ]* - 4/3 )7 341

` -7 # VA &03 Y '( * > , 9 A )7 A + Q )* Y '( 3 @34* 8 ( @34H3 V 7 !-03

* &]0 * 8 ( Y 5 G @c Q *

. 3.Q

8 ( )* 9 0 r3 )7 6 S 6 R 3 >3 !-03 0 4*

@ A

* * Y '( ) .N# @ *

&03 V8 .

!Q( & Q L' l 4M )LQ T -

>e (

!Q(

- -* >3 > 7 "/

- 647 3 >3 > 7 "/

>3 54* 0 4* )* J A 3 9 < 2345

V E ( 3 * Y U X5

&03 )-53 4F A .

LQ T 3 &03 3

3 Y

> # @ A &8H 3 @3

( VA34' &'( 3 VQ 0 4- 7 .

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42. 41.Vatanparast, M., Hosseininaveh, V., (2010). Digestive amylase and pectinase activity in the larvae of alfalfa weevil Hypera postica (Col.: Curculionidae). Entomological Research, 40 (6), 328-335.

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