ناﺮﯾا ﯽﺳﺎﻨﺷ ﺖﺴﯾز ﯽﻠﻠﻤﻟا ﻦﯿﺑ ﺲﻧاﺮﻔﻨﮐ ﻦﯿﻤﺠﻨﭘ و يﺮﺳاﺮﺳ ﺲﻧاﺮﻔﻨﮐ ﻦﯿﻤﻫﺪﻔﻫ
ﯽﻧﺎﺳاﺮﺧ ﻪﻨﻣرد هﺪﺷ ﯽﯾاززﺎﺑ يﺎﻫ ﻪﺧﺎﺷﻮﻧ رد ﻦﯿﻨﯾﺰﯿﻤﺗرآ راﺪﻘﻣ ﻦﯿﯿﻌﺗ
هدازرﺪﯿﺣ ﺎﯾﺮﺛ
1
نﺎﯿﺒﺟر ﻪﺒﯿﻃ ،*
،2
ﺪﻨﻤﯾﺎﺟ رﺎﮑﻣﺎﮐ هداز ﯽﻘﺗ دﻮﻌﺴﻣ ،2
1
-1 اد ﻪﺘﺧﻮﻣآ ﺶﻧ ﻪﺘﺷر
ﯽﺳﺎﻨﺷ ﺖﺴﯾز ،
ﻪﯾﺎﭘ مﻮﻠﻋ هﺪﮑﺸﻧاد
، ناﺮﻬﺗ ،ﺪﻫﺎﺷ هﺎﮕﺸﻧاد
، ناﺮﯾا
2 - ﯽﺳﺎﻨﺷ ﺖﺴﯾز هوﺮﮔ رﺎﯾدﺎﺘﺳا
، ﻪﯾﺎﭘ مﻮﻠﻋ هﺪﮑﺸﻧاد ،
ناﺮﻬﺗ ،ﺪﻫﺎﺷ هﺎﮕﺸﻧاد
، ناﺮﯾا
3 -
،ﻊﺗاﺮﻣ و ﺎﻫ ﻞﮕﻨﺟ تﺎﻘﯿﻘﺤﺗ ﻪﺴﺳﻮﻣ ،ﯽﯾوراد نﺎﻫﺎﯿﮔ هوﺮﮔ رﺎﯿﺸﻧاد ناﺮﻬﺗ
، ناﺮﯾا
[email protected] mail:
- E
هﺪﯿﮑﭼ
ﯽﻧﺎﺳاﺮﺧ ﻪﻨﻣرد )
ﯽﻨﺳﺎﮐ ﻞﮔ هﺮﯿﺗ (
ﺖﺳا ﻦﯿﻨﯾﺰﯿﻤﺗرآ ﺲﻨﺟ ﻦﯾا لﺎﻌﻓ ﺐﯿﮐﺮﺗ .ﺪﺷﺎﺑ ﯽﻣ ناﺮﯾا يرﺎﺼﺤﻧا ﻪﻨﻣرد ﻪﻧﻮﮔ ود زا ﯽﮑﯾ رد ﻪﮐ
ﺮﯿﮔ ﯽﻣ راﺮﻗ هدﺎﻔﺘﺳا درﻮﻣ نﺎﻃﺮﺳ و ﺎﯾرﻻﺎﻣ نﺎﻣرد د
ﻦﯾا هﺪﻤﻋ فﺪﻫ . ﺪﯿﻟﻮﺗ ياﺮﺑ ﺖﻓﺎﺑ ﺖﺸﮐ يدﺮﺑرﺎﮐ شور ﮏﯾ يراﺬﮔ ﻪﯾﺎﭘ ﻪﻌﻟﺎﻄﻣ
ﺖﺸﮐ اﺪﺟ .دﻮﺑ ﯽﻧﺎﺳاﺮﺧ ﻪﻨﻣرد هﺪﺷ ﯽﯾاززﺎﺑ يﺎﻫ ﻪﺧﺎﺷﻮﻧ رد ﻦﯿﻨﯾﺰﯿﻤﺗرآ ﻊﯿﺳو سﺎﯿﻘﻣ رد و ﻊﯾﺮﺳ يﺎﻫ
ﺖﺸﮐ ﻂﯿﺤﻣ رد ﻪﺧﺎﺷﻮﻧ
ﻪﯾﺎﭘ يﺎﻫ نﻮﻣرﻮﻫ ﻒﻠﺘﺨﻣ يﺎﻫ ﺖﻈﻠﻏ يﻮﺘﺤﻣ
MSﻦﯾرﻮﭘ ﻮﻨﯿﻣآ ﻞﯾﺰﻨﺑ )
،( BAP ) ﺪﯿﺳا ﮏﯿﺘﺳا ﻦﻟﺎﺘﻔﻧ ( NAA
و ﯿﺘﻨﯿﮐ ) ﻦ ( KIN
زا ﻦﯿﻨﯾﺰﯿﻤﺗرآ .ﺪﻧﺪﺷ هداد ﺖﺸﮐ هﺪﺷ ﯽﯾاززﺎﺑ يﺎﻫ ﻪﺧﺎﺷﻮﻧ
و جاﺮﺨﺘﺳا راﺪﻘﻣ
شور ﻪﺑ نآ ﺠﻨﺳ HPLC
ﺶ ﺸﯿﺑ .ﺪﺷ ﻦﯾﺮﺘ
ﻂﯿﺤﻣ رد ﯽﯾاز ﻪﺧﺎﺷﻮﻧ يﺎﻫ
يوﺎﺣ ﺖﺸﮐ 025 mg/L
/0 + NAA 2 mg/L
BAP ) 01 / 0 23 ± /0 ﮏﺸﺧ هدﺎﻣ مﺮﮔ (
ﺰﯿﻧ و
يوﺎﺣ ﺖﺸﮐ ﻂﯿﺤﻣ mg/L
025 /0 + NAA mg/L 2
) KIN 01 / 0 16 ± /0 ﮏﺸﺧ هدﺎﻣ مﺮﮔ (
.ﺪﺷ هﺪﻫﺎﺸﻣ راﺪﻘﻣ ﻦﯾﺮﺘﺸﯿﺑ
ﻦﯿﻨﯾﺰﯿﻤﺗرآ )
73 /0 مﺮﮔ رد 100 ﮏﺸﺧ هدﺎﻣ مﺮﮔ (
رد رد هﺪﺷ ﯽﯾاززﺎﺑ يﺎﻫ ﻪﺧﺎﺷﻮﻧ ﻂﯿﺤﻣ
يﺎﻫ ﺖﺸﮐ يوﺎﺣ mg/L 01 /0 NAA
+ 4 mg/L و BAP
mg/L 025 /0 + NAA 2 mg/L
KIN ﺪﻣآ ﺖﺳﺪﺑ شور .
ﺮﺿﺎﺣ ﯽﺷور ﺪﻣآرﺎﮐ ياﺮﺑ ﯽﯾاززﺎﺑ زا ﺎﻫ ﻪﺧﺎﺷﻮﻧ
ﺖﺸﮐاﺪﺟ تﺎﻌﻄﻗ ﻮﻧ
ﯽﻧﺎﺳاﺮﺧ ﻪﻨﻣرد ﻪﺧﺎﺷ و
ﻦﯿﻨﯾﺰﯿﻤﺗرآ ﺪﯿﻟﻮﺗ .دﻮﻤﻧ ﻪﺋارا ار ﺎﻫ نآ رد
تﺎﻤﻠﮐ ﺪﯿﻠﮐ ي
ﻻﺎﺑ ﯽﯾآرﺎﮐ ﺎﺑ ﻊﯾﺎﻣ ﯽﻓاﺮﮔﻮﺗﺎﻣوﺮﮐ ،ﻦﯿﻨﯾﺰﯿﻤﺗرآ ،ﯽﯾاز ﻪﺧﺎﺷﻮﻧ ،ﺖﻓﺎﺑ ﺖﺸﮐ ،ﯽﻧﺎﺳاﺮﺧ ﻪﻨﻣرد :
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ﯽﺳﺎﻨﺷ ﺖﺴﯾز ﯽﻠﻠﻤﻟا ﻦﯿﺑ ﺲﻧاﺮﻔﻨﮐ ﻦﯿﻤﺠﻨﭘ و يﺮﺳاﺮﺳ ﺲﻧاﺮﻔﻨﮐ ﻦﯿﻤﻫﺪﻔﻫ ناﺮﯾا
Determination of Artemisinin in Regenerated Shoots of Artemisia khorrasanica
Soraya Heidarzadeh1*, Tayebeh Radjabian1, Kamkar Jaimand2, Massoud Taghizadeh1
1Department ofBiology, Shahed University, Tehran, Iran
2Departmentof Medicinal Plants, Research Institute of Forests and Rangelands, Tehran, Iran E-mail: [email protected]
Abstract
Artemisia khorassanica (Asteraceae) is one of the two indigenous Artemisia species in Iran. The active component of this genus is artemisinin, which is used in malaria and cancer therapy. In this study, we made an effort to establish a practical tissue culture method for rapid and large scale production of artimisinin in regenerated shoots of A. khorassanica. Shoot explants were cultured in basal MS medium supplemented with different concentrations of naphthalene acetic acid (NAA), benzylaminopurine (BAP) and kinetin (KIN). Extraction of artemisinin from regenerated shoots was performed on Soxhlet using ethanol as solvent. The amount of artemisinin in exracts was determined by HPLC technique. The highest shoot regeneration was observed in media containing 0.025 mg/L NAA+ 2 mg/L BAP (0.23± 0.01 g DW) and 0.025 mg/L NAA +2 mg/L KIN (0.16± 0.01 g DW). Our quantitative analysis showed that the highest amounts of artemisinin in regenerated shoots were obtained in media supplemented with 0.01 mg/L NAA + 4 mg/L BAP and 0.025 mg/L NAA + 2 mg/L KIN (0.073 g/100g DW). This procedure exhibited a potential alternative for rapid regeneration of shoots from the shoot explants of A. khorassanica, as well as in vitro production of artemisinin.
Key words: Artemisia khorrasanica, Tissue culture, Shoot regeneration, Artemisinin, HPLC
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