Iranian Journal of War and Public Health Volume 6, Issue 5, 2014
Pages: 183-187 Type: Original Research
*Corresponding Author Tel: +982122180004 Fax: +982122180004
Address: Iranian Research Center on Aging, Social Welfare and Rehabilitation Sciences University, Koodakyar Street, Daneshjoo Boulevard, Tehran, Iran
Correlation of Oxidative Stress Indices with Age in Chemical Veterans with Severe Pulmonary Complication; Longtime after
Exposition to Sulfur Mustard
Soroush M.R.1 MD, MPH, Faday Vatan R.* MD, MPH, PhD, Sahaf R.2 MD, MHSc, PhD, Taravati A.3 PhD, Ghazanfari T.4 PhD, Faghihzadeh S.5 PhD, Kaboudiyan Ardestani S.6 PhD
*Iranian Research Center on Aging, Social Welfare and Rehabilitation Sciences University, Tehran, Iran
1Janbazan Medical and Engineering Research Center, Tehran, Iran
2Iranian Research Center on Aging, Social Welfare and Rehabilitation Sciences University, Tehran, Iran
3Cell and Molecular Biology Department, Basic Sciences Faculty, University of Mazandaran, Babolsar, Iran
4Immunoregulation Research Center, Shahed University, Tehran, Iran
5Biostatistics Department, Statistics Faculty, Zanjan University of Medical Sciences, Zanjan, Iran
6Institute of Biochemistry and Biophysics, Tehran University, Tehran, Iran
Abstract
Aims: Aging is the result of accumulation of different detrimental changes in cells and tissues that increase the risk of disease and death with aging.
Irreversible and progressive oxidative hindrance caused by “reactive oxygen species” is effective in aging. This study examined the association of age with oxidative stress indices in patients with severe pulmonary lesions by measuring the levels of these indices, 15-20 years after exposure.
Materials & Methods: This cross-sectional study was done on 289 veterans with severe pulmonary lesions and 66 identical normal subjects in 2008. Blood samples were taken in the fasting state. ELISA method was used for measuring carbonyl protein and serum total antioxidant, Thiobarbituric Acid reaction for lipid peroxidation and FOX2 for total peroxide concentration of plasma samples. Differences between groups were measured by Student T test using SPSS 18 software.
Findings: No significant differences were observed in levels of Carbonyl protein and oxidative stress index (the ratio of total antioxidant to total peroxide) between two patient and control groups, while total peroxide level of patient group was significantly (p<0.001) less than control group. No significant correlation was seen between age and oxidative stress indices in both groups (p>0.05).
Conclusion: In order to eliminate and control the response to mustard gas in bodies of chemical injured people, total antioxidant increases and total peroxide decreases and oxidative stress index remains unchanged.
Keywords
Pulmonary Medicine [http://www.ncbi.nlm.nih.gov/mesh/68015272];
Mustard Gas [http://www.ncbi.nlm.nih.gov/mesh/68009151];
Antioxidants [http://www.ncbi.nlm.nih.gov/mesh/68000975];
Peroxides [http://www.ncbi.nlm.nih.gov/mesh/68010545];
Oxidative Stress [http://www.ncbi.nlm.nih.gov/mesh/68018384]
۱۸۴ ﻥﺍﺭﺎﮑﻤﻫ ﻭ ﺵﻭﺮﺳ ﺎﺿﺭﺪﻤﺤﻣ ـــــ
ـــــــــــــــــــــــــ ــــــ
ـ ـــــــــــــــــــــــــــــــــــــــــــــــــــــــــ
ﻲﮕﺘﺴﺒﻤﻫ ﺺﺧﺎﺷ
ﯼﺎﻫ ﺴﻛﺍ ﺱﺮﺘﺳﺍ ﺎ
ﻳ ﯽﺸ ﻦﺳ ﺎﺑ ﺭﺩ
ﻣﻭﺪﺼﻣ ﺎ ﻥ ﯽﻳﺎﻴﻤﻴﺷ ﻱﻮﻳﺭ
ﺪﻳﺪﺷ
؛ ﻝﺎﺳ ﺎﻫ ﻪﻬﺟﺍﻮﻣ ﺯﺍ ﺲﭘ
ﻝﺩﺮﺧ ﺯﺎﮔ ﺎﺑ
ﺵﻭﺮﺳ ﺎﺿﺭﺪﻤﺤﻣ MPH
MD ،
ﻥﺍﺮﻳﺍ ،ﻥﺍﺮﻬﺗ ،ﻥﺍﺭﺎﺒﻧﺎﺟ ﻲﮑﺷﺰﭘ ﻡﻮﻠﻋ ﻭ ﻲﺳﺪﻨﻬﻣ ﺕﺎﻘﻴﻘﺤﺗ ﺰﮐﺮﻣ
ﺎﺿﺭ ﻦﻃﻭ ﻱﺍﺪﻓ
* MD MPH ، PhD ،
ﺕﺎﻘﻴﻘﺤﺗ ﺰﮐﺮﻣ ﺪﻨﻤﻟﺎﺳ ﺕﺎﻘﻴﻘﺤﺗ ﺰﮐﺮﻣ
،ﯼ ﺰﻬﺑ ﻡﻮﻠﻋ ﻩﺎﮕﺸﻧﺍﺩ ﻳ
ﺘﺴ ﻲ ﺸﺨﺒﻧﺍﻮﺗ ﻭ ﻲ
،
،ﻥﺍﺮﻬﺗ ﻥﺍﺮﻳﺍ
ﻑﺎﺤﺻ ﺏﺎﺑﺭ MD
MHSc ، PhD ،
ﺕﺎﻘﻴﻘﺤﺗ ﺰﮐﺮﻣ ﺪﻨﻤﻟﺎﺳ ﺕﺎﻘﻴﻘﺤﺗ ﺰﮐﺮﻣ
،ﯼ ﺰﻬﺑ ﻡﻮﻠﻋ ﻩﺎﮕﺸﻧﺍﺩ ﻳ
ﺘﺴ ﻲ ﺸﺨﺒﻧﺍﻮﺗ ﻭ ﻲ
،
،ﻥﺍﺮﻬﺗ ﻥﺍﺮﻳﺍ ﻲﺗﻭﺍﺮﻃ ﻲﻠﻋ PhD
ﻩﻭﺮﮔ ﺖﺴﻳﺯ ﯽﺳﺎﻨﺷ ﻲﻟﻮﮑﻟﻮﻣ ﻭ ﻲﻟﻮﻠﺳ
، ﻪﻳﺎﭘ ﻡﻮﻠﻋ ﻩﺪﮑﺸﻧﺍﺩ
، ﻥﺍﺭﺪﻧﺯﺎﻣ ﻩﺎﮕﺸﻧﺍﺩ ،
ﺮﺴﻠﺑﺎﺑ ﻥﺍﺮﻳﺍ ، ﻱﺮﻔﻨﻀﻏ ﻲﺑﻮﻃ PhD
ﺦﺳﺎﭘ ﻢﻴﻈﻨﺗ ﺕﺎﻘﻴﻘﺤﺗ ﺰﮐﺮﻣ ﺪﻫﺎﺷ ﻩﺎﮕﺸﻧﺍﺩ ،ﻲﻨﻤﻳﺍ ﻱﺎﻫ
ﻥﺍﺮﻳﺍ ،ﻥﺍﺮﻬﺗ ،
ﻪﻴﻘﻓ ﻁﺍﺮﻘﺳ ﻩﺩﺍﺯ
PhD
ﻲﺗﺎﻴﺣ ﺭﺎﻣﺁ ﻩﻭﺮﮔ
،ﺭﺎﻣﺁ ﻩﺪﮑﺸﻧﺍﺩ ، ﻥﺎﺠﻧﺯ ﻲﮑﺷﺰﭘ ﻡﻮﻠﻋ ﻩﺎﮕﺸﻧﺍﺩ
ﻥﺍﺮﻳﺍ ،ﻥﺎﺠﻧﺯ ،
ﻲﻧﺎﺘﺳﺩﺭﺍ ﻥﺎﻴﻧﺍﺩﻮﺒﮐ ﻦﺳﻮﺳ PhD
ﺰﮐﺮﻣ ﻴﻘﺤﺗ ﻘ ﺗﺎ ﯽ ﮏﻳﺰﻴﻓﻮﻴﺑ ﻭ ﻲﻤﻴﺷﻮﻴﺑ
، ﻥﺍﺮﻬﺗ ﻩﺎﮕﺸﻧﺍﺩ ﻥﺍﺮﻳﺍ ،ﻥﺍﺮﻬﺗ ،
ﻩﺪﻴﮑﭼ
ﻑﺍﺪﻫﺍ ﻝﻮﻠﺳ ﺭﺩ ﻥﻮﮔﺎﻧﻮﮔ ﺮﻀﻣ ﺕﺍﺮﻴﻴﻐﺗ ﻊﻤﺠﺗ ﻞﺻﺎﺣ ﻱﺮﻴﭘ ﻩﺪﻳﺪﭘ: ﻭ ﺎﻫ
ﺖﻓﺎﺑ ﺶﻳﺍﺰﻓﺍ ﺍﺭ ﮒﺮﻣ ﻭ ﻱﺭﺎﻤﻴﺑ ﻪﺑ ﻼﺘﺑﺍ ﺮﻄﺧ ،ﻦﺳ ﻦﺘﻓﺭﻻﺎﺑ ﺎﺑ ﻪﻛ ﺖﺳﺎﻫ
ﻲﻣ ﺪﻫﺩ ﺐﻳﺮﺨﺗ . ﺖﺸﮔﺮﺑ ﻲﺸﻳﺎﺴﻛﺍ ﻱﺎﻫ ﻂﺳﻮﺗ ﻪﮐ ﻩﺪﻧﻭﺮﺸﻴﭘ ﻭ ﺮﻳﺬﭘﺎﻧ
ﻪﻧﻮﮔ"
ﺶﻨﻛﺍﻭ ﻱﺎﻫ ﻲﻣ ﺩﺎﺠﻳﺍ "ﻥﮋﻴﺴﻛﺍ ﻩﺪﻨﻫﺩ
ﺭﺍﺬﮔﺮﺛﺍ ﻱﺮﻴﭘ ﺪﻨﻳﺁﺮﻓ ﺭﺩ ﺪﻧﻮﺷ
ﺎﺑ ﻪﻌﻟﺎﻄﻣ ﻦﻳﺍ .ﺪﻨﺘﺴﻫ ﻩﺯﺍﺪﻧﺍ
ﻱﺮﻴﮔ ﻥﺍﺰﻴﻣ ﺺﺧﺎﺷ ﯼﺎﻫ ﺴﻛﺍ ﺱﺮﺘﺳﺍ ﯽﺸﻳﺎ ﺭﺩ
ﻥﺎﻣﻭﺪﺼﻣ ﺪﻳﺪﺷ ﻱﻮﻳﺭ ﯽﺳﺭﺮﺑ ﻪﺑ
ﻦﺳ ﻁﺎﺒﺗﺭﺍ ﺎﺑ
ﺺﺧﺎﺷ ﻦﻳﺍ ١٥،ﺎﻫ
ﺎﺗ
ﻝﺎﺳ٢٠ ﻮﻣ ﺯﺍ ﺪﻌﺑ ﺖﺧﺍﺩﺮﭘ ﻪﻬﺟﺍ .
ﻭ ﺩﺍﻮﻣ ﺵﻭﺭ :ﺎﻫ ﺭﺩ ﻲﻌﻄﻘﻣ ﻪﻌﻟﺎﻄﻣ ﻦﻳﺍ ﻲﻳﺎﻴﻤﻴﺷ ﺯﺎﺒﻧﺎﺟ ۲۸۹
ﯼﺍﺭﺍﺩ
ﻱﻮﻳﺭ ﺪﻳﺪﺷ ﻪﻌﻳﺎﺿ ﻭ
ﻝﺎﺳ ﺭﺩ ﻥﺎﺴﻤﻫ ﯼﺩﺎﻋ ﺩﺮﻓ ۶۶ ﻡﺎﺠﻧﺍ ۱۳۸۷
ﺪﺷ .
ﻪﻧﻮﻤﻧ ﺮﻴﮔ ﯼ ﻥﻮﺧ ﺎﺘﺷﺎﻧ ﺖﻟﺎﺣ ﺭﺩ .ﺪﻣﺁ ﻞﻤﻋ ﻪﺑ
ﻞﻴﻧﻮﺑﺮﻛ ﻱﺍﻮﺘﺤﻣ ﻭ
ﻲﺘﻧﺁ ﻡﺮﺳ ﻡﺎﺗ ﻥﺍﺪﻴﺴﻛﺍ ﺵﻭﺭ ﻪﺑ
ﻻﺍ ﺍﺰﻳ ، ﻱﺪﻴﭙﻴﻟ ﻥﻮﻴﺳﺍﺪﻴﺴﻛﺍﺮﭘ ﺎﺑ
ﺵﻭﺭ
ﻚﻳﺭﻮﺘﻴﺑﺭﺎﺑﻮﻴﺗ ﺶﻨﻛﺍﻭ ﺪﻴﺳﺍ
ﻭ ﻪﻧﻮﻤﻧ ﻡﺎﺗ ﺪﻴﺴﻛﺍﺮﭘ ﺖﻈﻠﻏ ﺎﺑ ﻲﻳﺎﻤﺳﻼﭘ ﻱﺎﻫ
ﺵﻭﺭ ﺪﺷ ﻦﻴﻴﻌﺗFOX2
ﻩﻭﺮﮔ ﻦﻴﺑ ﺕﻭﺎﻔﺗ . ﻥﻮﻣﺯﺁ ﺯﺍ ﻩﺩﺎﻔﺘﺳﺍ ﺎﺑ ﺎﻫ
ﯼﺎﻫ
ﯼﺭﺎﻣﺁ ﺖﻧﺩﻮﺘﺳﺍT ﻪﺑ ﻡﺮﻧ ﻚﻤﻛ ﺭﺍﺰﻓﺍ SPSS 18 .ﺪﺷ ﺶﺠﻨﺳ
ﻪﺘﻓﺎﻳ :ﺎﻫ ﻨﻌﻣ ﻑﻼﺘﺧﺍ ﯽ
ﺭﺍﺩ ﯼ ﺺﺧﺎﺷ ﻭ ﻞﻴﻧﻮﺑﺮﻛ ﻦﻴﺌﺗﻭﺮﭘ ﻥﺍﺰﻴﻣ ﺭﺩ
ﺸﻳﺎﺴﻛﺍ ﺱﺮﺘﺳﺍ ﯽ
ﺖﺒﺴﻧ) ﻲﺘﻧﺁ ﻩﻭﺮﮔ ﻭﺩ ﻦﻴﺑ (ﻡﺎﺗ ﺪﻴﺴﮐﺍﺮﭘ ﻪﺑ ﻡﺎﺗ ﻥﺍﺪﻴﺴﮐﺍ
ﻲﻟﺎﺣﺭﺩ ،ﺪﺸﻧ ﻩﺪﻫﺎﺸﻣ ﻝﺮﺘﻨﮐ ﻭ ﺭﺎﻤﻴﺑ ،ﺭﺎﻤﻴﺑ ﻩﻭﺮﮔ ﻡﺎﺗ ﺪﻴﺴﻛﺍﺮﭘ ﻥﺍﺰﻴﻣ ﻪﮐ
ﻪﺑ ﻲﻨﻌﻣ ﺭﻮﻃ ) ﻱﺭﺍﺩ ۰۰۱ / ۰
<
ﺩﻮﺑ ﻝﺮﺘﻨﻛ ﻩﻭﺮﮔ ﺯﺍ ﺮﺘﻤﮐ (p ﯽﮕﺘﺴﺒﻤﻫ ﭻﻴﻫ .
ﯽﻨﻌﻣ ﺺﺧﺎﺷ ﻭ ﻦﺳ ﻦﻴﺑ ﯼﺭﺍﺩ ﻩﻭﺮﮔ ﻭﺩ ﺮﻫ ﺭﺩ ﯽﺸﻳﺎﺴﮐﺍ ﺱﺮﺘﺳﺍ ﯼﺎﻫ
) ﺪﺸﻧ ﻩﺪﻫﺎﺸﻣ ۰۵
/ ۰ p>
.(
ﻪﺠﻴﺘﻧ :ﻱﺮﻴﮔ ﻪﺑ ﺭﺩ ﻝﺩﺮﺧ ﺯﺎﮔ ﻪﺑ ﻩﺪﺷﺩﺎﺠﻳﺍ ﺦﺳﺎﭘ ﻝﺮﺘﻨﮐ ﻭ ﻑﺬﺣ ﺭﻮﻈﻨﻣ
ﻲﺘﻧﺁ ،ﻲﻳﺎﻴﻤﻴﺷ ﻥﺎﻣﻭﺪﺼﻣ ﻥﺪﺑ ﺶﻫﺎﮐ ﻡﺎﺗ ﺪﻴﺴﮐﺍﺮﭘ ﻭ ﺶﻳﺍﺰﻓﺍ ﻡﺎﺗ ﻥﺍﺪﻴﺴﮐﺍ
ﻣ ﯽ ﺪﺑﺎﻳ ﺸﻳﺎﺴﮐﺍ ﺱﺮﺘﺳﺍ ﺺﺧﺎﺷ ﻭ ﯽ
ﻗﺎﺑ ﺮﻴﻴﻐﺗ ﻥﻭﺪﺑ ﯽ
ﻣ ﯽ .ﺪﻧﺎﻣ ﺍﻭﺪﻴﻠﮐ
ﮊ ﻩ ﺎﻫ ﺭ ﺐﻃ : ﻳ ﻮ ﯼ
؛ ﻝﺩﺮﺧ ﺯﺎﮔ
؛ ﻲﺘﻧﺁ ﻥﺍﺪﻴﺴﮐﺍ ﺎﻫ
؛ ﺪﻴﺴﮐﺍﺮﭘ ﺎﻫ
؛ ﺱﺮﺘﺳﺍ
ﺴﮐﺍ
ﯽﺸﻳﺎ
ﺦﻳﺭﺎﺗ :ﺖﻓﺎﻳﺭﺩ ۳۱ / ۰۲ / ۱۳۹۳
:ﺵﺮﻳﺬﭘ ﺦﻳﺭﺎﺗ ۰۵
/ ۰۳ / ۱۳۹۳
:ﻝﻮﺌﺴﻣ ﻩﺪﻨﺴﻳﻮﻧ* [email protected]
ﻪﻣﺪﻘﻣ
ﻩﺩﺎﻔﺘﺳﺍ ﮓﻨﺟ ﺭﺩ ﻲﻳﺎﻴﻤﻴﺷ ﻞﻣﺍﻮﻋ ﺯﺍ ﻪﺑ ﺎﻫ
ﺭﺩ ﺮﺛﻮﻣ ﺡﻼﺳ ﻥﺍﻮﻨﻋ
ﻥﺍﻮﺗﺎﻧ ﻱﺎﭘﺯﺍ ﺎﻳ ﻥﺩﺮﻛ .ﺩﺭﺍﺩ ﻪﻨﻳﺮﻳﺩ ﻲﺘﻣﺪﻗ ,ﻦﻤﺷﺩ ﻥﺩﺭﻭﺁﺭﺩ
ﯽﻃ ﮓﻨﺟ
ﻭ ﻥﺍﺮﻳﺍ ﺯﺍ ﺶﻴﺑ ﺰﻴﻧ ﻕﺍﺮﻋ ﻠﻤﺣ ﺩﺭﻮﻣ٣٠٠
ﻪ ﻲﻳﺎﻴﻤﻴﺷ ﻕﺍﺮﻋ ﺐﻧﺎﺟ ﺯﺍ
ﻥﺍﺮﻳﺍ ﻪﺑ ﻪﮐ ﺖﻓﺮﮔ ﺕﺭﻮﺻ ﺩﻭﺪﺣ
ﻲﻳﺎﻴﻤﻴﺷ ﻡﻭﺪﺼﻣ ﺭﺍﺰﻫ١٠٠ ﻪﺑ
ﯼﺎﺟ
ﺖﺷﺍﺬﮔ ﻭ ﺮﺿﺎﺣ ﻝﺎﺣ ﺭﺩ ،ﺰﻴﻧ
ﺯﺍ ﺶﻴﺑ ﺯﺍ ﻲﻣﺎﻈﻧﺮﻴﻏ ﻭ ﻲﻣﺎﻈﻧ ﺭﺍﺰﻫ٥٠
ﻲﻣ ﺞﻧﺭ ﻲﻳﺎﻴﻤﻴﺷ ﺖﻴﻣﻭﺪﺼﻣ ﺯﺍ ﻲﺷﺎﻧ ﺱﺭﺮﻳﺩ ﺽﺭﺍﻮﻋ ﺪﻧﺮﺑ
] ١ .[
ﻴﻧﺎﻜﻣ ﺰ ﻲﻟﻮﻠﺳ ﺖﻴﻣﻮﻤﺴﻣ ﻡ ﺎﺑ
ﺑ ﻝﺩﺮﺧ ﺯﺎﮔ ﻪ
.ﺖﺴﻴﻧ ﺺﺨﺸﻣ ﻲﺑﻮﺧ
ﺍﺭ ﺪﻴﻟﻮﺗ ﻱﻮﻗ ﻝﺎﻤﺘﺣﺍ ﻚﻳ ﻝﺎﻜﻳﺩ
ﺭﺩ ﻥﮊﻭﺮﺘﻴﻧ ﻭ ﻥﮋﻴﺴﻛﺍ ﻝﺎﻌﻓ ﻱﺎﻫ
ﻝﻮﻜﻟﻮﻣ ﺎﺑ ﻪﻛ ﺖﺳﺍ ﻝﻮﻠﺳ ﻞﻴﺒﻗ ﺯﺍ ﻑﺪﻫ ﻱﺎﻫ
ﺪﻴﭙﻴﻟ ,ﻦﻴﺌﺗﻭﺮﭘ ,DNA
ﻲﻣ ﺶﻨﻛﺍﻭ ﺕﺍﺭﺪﻴﻫﻮﺑﺮﻛ ﻭ ﻫﺩ
ﻨ ﺶﻨﻛﺍﻭ ﻦﻳﺍ ﻪﺠﻴﺘﻧ .ﺪ ,ﺯﻭﺮﻜﻧ ﺩﺎﺠﻳﺍ ﺎﻫ
ﻢﻳﺰﻧﺁ ﺖﻴﻟﺎﻌﻓ ﺭﺩ ﺮﻴﻴﻐﺗ ﻭ ﺏﺎﻬﺘﻟﺍ ﻲﺘﻧﺁ ﻱﺎﻫ
ﻥﺍﺪﻴﺴﻛﺍ
ﻥﻮﻴﺗﺎﺗﻮﻠﮔ ،ﺯﺎﺗﻮﻤﺳﺩﺪﻴﺴﻛﺍﺮﭘﻮﺳ) (ﺯﻻﺎﺗﺎﻛ ﻭ ﺯﺍﺪﻴﺴﻛﺍﺮﭘ
ﮐ ﺖﺳﺍ ﺎﺑ ﻪ
ﻪﻳﺰﺠﺗ ﻲﺘﻧﺁ ﺩﺍﻮﻣ ) ﻥﺍﺪﻴﺴﮐﺍ ﻦﻴﻣﺎﺘﻳﻭ ﯼﺎﻫ ،A ،E ﺎﺘﺑ ،C ﺎﻔﻟﺁ ﻭ - -
ﻲﻠﻴﺑ ،ﻚﻳﺭﻭﺍﺪﻴﺳﺍ ،ﻦﻴﺗﻭﺭﺎﻛ ﻡﻮﻴﻨﻴﻠﺳ ،ﻥﻮﻴﺗﺎﺗﻮﻠﮔ ،ﻦﻴﺑﻭﺭ
ﻭ ﻩﺮﻴﻏ (
ﻝﺎﻜﻳﺩﺍﺭ ﺩﺍﺯﺁ ﻱﺎﻫ ﻩﺩﺮﮐ ﺪﻴﻟﻮﺗ ﯼﺮﺘﺸﻴﺑ ﺴﻛﺍ ﺱﺮﺘﺳﺍ ﻭ
ﺎﻳ ﺪﻳﺪﺸﺗ ﺍﺭ ﯽﺸ
ﯽﻣ ] ﺪﻨﻨﮐ ٢ [.
ﺭﺩ ﯽﺧﺮﺑ ﻲﻧﺍﻮﻴﺣ ﺕﺎﻌﻟﺎﻄﻣ ﻩﺩﺍﺩ ﻥﺎﺸﻧ
ﻝﺩﺮﺧ ﺯﺎﮔ ﻪﻛ ﻩﺪﺷ ﺯﻭﺩ)
۵ / ۰ LD50 (ﺮﺘﻤﮐ ﻭ ﻥﻮﻴﺳﺍﺪﻴﺴﻛﺍﺮﭘ ﻭ ﺪﻴﭙﻴﻟ ﻞﻳﺎﻓﻭﺮﭘ ﺭﺩ ﺮﻴﻴﻐﺗ ﺐﺟﻮﻣ
ﺪﻴﭙﻴﻟ ﻢﻳﺰﻧﺁ ﻦﻴﻨﭽﻤﻫ ﻭ ﻲﺘﻧﺁ ﻱﺎﻫ
ﻲﻣ ﻥﺍﺪﻴﺴﻛﺍ ﻭ ﺩﻮﺷ
ﻲﻣﺎﮕﻨﻫ ﻪﮐ
ﻲﺘﻧﺁ ﺎﺑ ﺕﺎﻧﺍﻮﻴﺣ ﻥﺍﺪﻴﺴﻛﺍ
ﻲﻳﺍﺬﻏ ﻱﺎﻫ ﯽﻣ ﻪﻳﺬﻐﺗ
،ﺪﻧﻮﺷ ﺭﺩ ﺎﻫﺮﺘﻣﺍﺭﺎﭘ ﻦﻳﺍ
ﺡﻼﺻﺍ ﺎﻬﻧﺁ ﯽﻣ
]ﺩﻮﺷ ۳ [ ﻥﺎﺸﻧ ﺎﺑ . ﻩﺩﺍﺩ ﻥﺪﺷ ﻝﺎﻜﻳﺩﺍﺭ ﺶﻘﻧ ﺭﺩ ﺩﺍﺯﺁ ﻱﺎﻫ
ﮔ ﺯﺍ ﻲﺷﺎﻧ ﺽﺭﺍﻮﻋ ﺑ ﻝﺩﺮﺧ ﺯﺎ
ﻪ ﻭ ﺩﺎﺣ ﺕﺭﻮﺻ ﻝﺎﻌﻓ ﻭ
ﻱﺎﻫﺮﻴﺴﻣ ﻥﺪﺷ
ﻥﺪﺑ ﺭﺩ ﺎﻬﻧﺁ ﺪﻴﻟﻮﺗ
، ﯽﻣ ﺭﻮﻄﺧ ﻦﻫﺫ ﻪﺑ ﻪﻟﺎﺴﻣ ﻦﻳﺍ ﻪﮐ ﺪﻨﮐ
ﺽﺭﺍﻮﻋ
ﺯﺍ ﻲﺷﺎﻧ ﺱﺭﺮﻳﺩ ) ﻝﺩﺮﺧ ﺯﺎﮔ ﺎﺑ ﻪﻬﺟﺍﻮﻣ
ﺱﺭﺩﻭﺯ ﻱﺮﻴﭘ ﻭ
ﻲﺘﺣﺍﺭﺎﻧ ﻱﺎﻫ
ﻲﺒﻠﻗ ﻲﻤﺸﭼ ﻭ ﻲﺴﻔﻨﺗ ،ﻲﻗﻭﺮﻋ - ﻲﻣ ﺰﻴﻧ (
ﻧﺍﻮﺗ ﻨ ﺪ ﺮﺛﺍ ﺭﺩ ﺭﺩ ﺺﻘﻧ
ﻝﺎﻜﻳﺩﺍﺭ ﻝﺮﺘﻨﻛ ﻛﺮﺗ ﻦﻳﺍ ﻡﻭﺍﺪﻣ ﺪﻴﻟﻮﺗ ﻭ ﻝﺎﻌﻓ ﻱﺎﻫ
ﻥﺪﺑ ﺭﺩ ﺕﺎﺒﻴ
ﻣﻭﺪﺼﻣ ﺎ ﻥ ] ﺪﻧﻮﺷ ﺩﺎﺠﻳﺍ ۴
[ .
ﻱﺮﻴﭘ ﻩﺪﻳﺪﭘ ﻪﮐ ﺎﺠﻧﺁ ﺯﺍ ﻊﻤﺠﺗ ﻞﺻﺎﺣ
ﺕﻻﻮﺼﺤﻣ ﺭﺩ ﻥﻮﮔﺎﻧﻮﮔ ﺮﻀﻣ
ﻝﻮﻠﺳ ﺖﻓﺎﺑ ﻭ ﺎﻫ ﻭ ﻱﺭﺎﻤﻴﺑ ﻪﺑ ﻼﺘﺑﺍ ﺮﻄﺧ ،ﻦﺳ ﻦﺘﻓﺭﻻﺎﺑ ﺎﺑ ﻪﻛ ﺖﺳﺍ ﺎﻫ
ﻲﻣ ﺶﻳﺍﺰﻓﺍ ﺍﺭ ﮒﺮﻣ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ﻭ ﺪﻫﺩ
ﻪﮑﻨﻳﺍ ﺖﺸﮔﺮﺑ ﻊﻤﺠﺗ ﻭ ﺮﻳﺬﭘﺎﻧ
ﺐﻳﺮﺨﺗ ﻩﺪﻧﻭﺮﺸﻴﭘ ﺴﻛﺍ ﻱﺎﻫ
ﯽﺸﻳﺎ ﻂﺳﻮﺗ ﻩﺪﺷﺩﺎﺠﻳﺍ "
ﻪﻧﻮﮔ ﻱﺎﻫ
ﺶﻨﻛﺍﻭ ﻥﮋﻴﺴﻛﺍ ﻩﺪﻨﻫﺩ "
(ROS) ﺭﺍﺬﮔﺮﺛﺍ ﻱﺮﻴﭘ ﺪﻨﻳﺁﺮﻓ ﺭﺩ ﺘﺴﻫ
ﺪﻨ ] ۵ ،[
ﺎﺑ ﻪﻌﻟﺎﻄﻣ ﻦﻳﺍ ﻩﺯﺍﺪﻧﺍ
ﻱﺮﻴﮔ ﻥﺍﺰﻴﻣ ﺺﺧﺎﺷ ﯼﺎﻫ ﺴﻛﺍ ﺱﺮﺘﺳﺍ ﯽﺸﻳﺎ
ﺭﺩ
ـــــــــــــ ـــــــــــ
ــــ ﺺﺧﺎﺷ ﻲﮕﺘﺴﺒﻤﻫ ﺎﻫ
ﯼ ﺸﻳﺎﺴﻛﺍ ﺱﺮﺘﺳﺍ ﯽ
ﻳﺎﻴﻤﻴﺷ ﻥﺎﻣﻭﺪﺼﻣ ﺭﺩ ﻦﺳ ﺎﺑ ﯽ
ﻝﺎﺳ ؛ﺪﻳﺪﺷ ﻱﻮﻳﺭ ﻝﺩﺮﺧ ﺯﺎﮔ ﺎﺑ ﻪﻬﺟﺍﻮﻣ ﺯﺍ ﺲﭘ ﺎﻫ
۱۸۵
ﻥﺎﻣﻭﺪﺼﻣ ﺪﻳﺪﺷ ﻱﻮﻳﺭ ﯽﺳﺭﺮﺑ ﻪﺑ
ﻦﺳ ﻁﺎﺒﺗﺭﺍ ﺎﺑ
ﺺﺧﺎﺷ ﻦﻳﺍ
،ﺎﻫ ٢٠ -
ﻝﺎﺳ١٥ ﺖﺧﺍﺩﺮﭘ ﻪﻬﺟﺍﻮﻣ ﺯﺍ ﺪﻌﺑ .
ﻭ ﺩﺍﻮﻣ ﺵﻭﺭ ﺎﻫ
ﻱﺭﺍﺰﮔﺮﺑ ﻲﻃ ﻲﻌﻄﻘﻣ ﻪﻌﻟﺎﻄﻣ ﻦﻳﺍ ﺠﻨﺳﺯﺎﻴﻧ ﻪﻌﻟﺎﻄﻣ
ﯽ ﺖﻣﻼﺳ
ﺦﻳﺭﺎﺗ ﺭﺩ ﺪﻳﺪﺷ ﻱﻮﻳﺭ ﻥﺎﻣﻭﺪﺼﻣ ﻲﻟﺍ ٢٣
ﻩﺎﻣﺮﻬﻣ ٢٩ ﺭﺩ ١٣٨٧
،ﻥﺎﻬﻔﺻﺍ ﺭﺩ ﻲﻳﺎﻴﻤﻴﺷ ﻥﺍﺯﺎﺒﻧﺎﺟ ﻪﻴﻠﮐ ﺖﮐﺮﺷ
ﻪﮐ ﻩﺪﻨﻨﮐ ﺪﻳﺪﺷ ﻪﻌﻳﺎﺿ
ﻱﻮﻳﺭ
،ﺪﻨﺘﺷﺍﺩ ﺖﻛﺮﺷ ﻥﺎﻜﺳﺍ ﺯﺍ ﺲﭘ ﺯﻭﺭ ﻚﻳ .ﺖﻓﺮﮔ ﻡﺎﺠﻧﺍ ﻥﺎﮔﺪﻨﻨﻛ
ﺎﺘﺷﺎﻧ ﺖﻟﺎﺣ ﺭﺩ ﻭ ﻪﻌﻟﺎﻄﻣ ﺭﺩ
، ﺯﺍ ﻡﻭﺪﺼﻣ ٢٨٩ ﻭ ﺪﻳﺪﺷ ﻱﻮﻳﺭ ﺩﺮﻓ٦٦
ﯼﺩﺎﻋ ﻫ) ﻥﺎﻫﺍﺮﻤ ﺩﺮﻣ ﻥﺎﻬﻔﺻﺍ ﺭﺩ ﻦﻛﺎﺳ ﻢﻟﺎﺳ ﻥﺍﺩﺮﻣ ﻭ ﻥﺍﺯﺎﺒﻧﺎﺟ ﻢﻟﺎﺳ
(ﺪﻨﺘﺷﺍﺩ ﺭﺍﺮﻗ ﻥﺍﺯﺎﺒﻧﺎﺟ ﻲﻨﺳ ﻒﻴﻃ ﺭﺩ ﻪﻛ ﻪﻧﻮﻤﻧ
ﻪﺑ ﻲﻧﻮﺧ ﯼﺮﻴﮔ ﻞﻤﻋ
ﺪﻣﺁ ﻪﻟﻮﻟ ﺭﺩ ﻞﻣﺎﻛ ﻥﻮﺧ . ﻱﺎﻫ
ﻲﻠﻴﻣ١٠ ﻱﺮﺘﻴﻟ
، ﺩﺎﻘﻌﻧﺍﺪﺿ ﺩﺍﻮﻣ ﻥﻭﺪﺑ
،
ﻊﻤﺟ ﻥﻮﺧ .ﺪﺷ ﻱﺭﻭﺁ ﻭ ﻡﺎﺠﻧﺍ ﻥﺍﺯﺎﺒﻧﺎﺟ ﺖﻣﺎﻗﺍ ﻞﺤﻣ ﺭﺩ ﻱﺮﻴﮔ
ﺭﺩ ﺦﻳ
ﻪﺑ ﻧﻮﻤﻳﺍ ﻩﺎﮕﺸﻳﺎﻣﺯﺁ ﻪﺑ ﺖﻋﺮﺳ ﻥﺎﻬﻔﺻﺍ ﻲﻜﺷﺰﭘ ﻡﻮﻠﻋ ﻩﺎﮕﺸﻧﺍﺩ ﻱﮊﻮﻟﻮ
ﺯﺍ ﺲﭘ .ﺪﺷ ﻞﻘﺘﻨﻣ ﺖﺷﺬﮔ
ﻢﻴﻧ ﺩﻭﺪﺣ ﻪﺘﺨﻟ ﻭ ﺖﻋﺎﺳ ﻪﻟﻮﻟ ،ﻥﻮﺧ ﻥﺪﺷ
ﺎﻫ
ﻪﻘﻴﻗﺩ ﺭﺩ ﺭﻭﺩ٣٠٠٠ ﻔﻳﺮﺘﻧﺎﺳ
ﻴ ) ﮊﻮ Beckman ﺮﻣﺁ ؛
ﻳ ﺎﮑ ﺪﻧﺪﺷ( ﺯﺍ ﺲﭘ .
ﻔﻳﺮﺘﻧﺎﺳ ﻡﺎﻤﺗﺍ ﻴ
ﮊﻮ
، ﻡﺮﺳ ﺎﺑ ﻪﺑ ﺖﭙﻴﭘﻭﺮﻜﻴﻣ ﻢﺠﺣ ﻪﺑ ﻭ ﺍﺪﺟ ﻲﻣﺍﺭﺁ
ﻱﺎﻫ
ﻱﺍﺮﺑ ﺯﺎﻴﻧ ﺩﺭﻮﻣ ﺮﻳﺩﺎﻘﻣ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ) ﻲﺼﺨﺸﻣ ﺶﺠﻨﺳ
(ﻒﻠﺘﺨﻣ ﻱﺎﻫ
ﻭ ﻢﻴﺴﻘﺗ ﺭﺩ ٢٠°C ﺭﺩ ﻭ ﻞﻘﺘﻨﻣ ﻥﺍﺮﻬﺗ ﻪﺑ - ٧٠°C
ﻡﺎﺠﻧﺍ ﻥﺎﻣﺯ ﺎﺗ -
ﺕﺎﺸﻳﺎﻣﺯﺁ ﯼﺭﺍﺪﻬﮕﻧ .ﺪﺷ
ﯼﺍﺮﺑ ﻩﺯﺍﺪﻧﺍ ﻡﺮﺳ ﻞﻴﻧﻮﺑﺮﻛ ﻱﺍﻮﺘﺤﻣ ﻱﺮﻴﮔ ﺵﻭﺭ ﻪﺑ
ﻻﺍ ﺍﺰﻳ ) CELL
BIOLAB
،(ﻩﺪﺤﺘﻣ ﺕﻻﺎﻳﺍ ؛ ﺖﻴﻠﭘ
ﺎﻫ ﺎﺑ ﻝﻮﻠﺤﻣ ) ﺩﺭﺍﺪﻧﺎﺘﺳﺍ BSA
ﺪﻴﺴﻛﺍ ﻥﻮﻴﺳﺍ (ﺎﻴﺣﺍ ﻭ ﻪﻧﻮﻤﻧ ﺎﻳ ) ﻥﺍﺭﺎﻤﻴﺑ ﻡﺮﺳ ﻱﺎﻫ ﮑﻴﻣ١٠
ﺮﺑ ﻡﺮﮔﻭﺮ
ﯽﻠﻴﻣ ﺮﺘﻴﻟ ﻪﺑ ( ﺭﺩ ﺐﺷ ﻚﻳ ﺕﺪﻣ ٤°C
ﺩﻭﺪﻧﺍ ﺪﺷ .ﺪﻧ ﻪﺑ ،ﻪﺻﻼﺧ ﺭﻮﻃ
ﻪﻧﻮﻤﻧ ﻦﻴﺌﺗﻭﺮﭘ ﺭﺩ ﺩﻮﺟﻮﻣ ﻞﻴﻧﻮﺑﺮﻛ ﻡﺮﺳ
ﺩﺭﺍﺪﻧﺎﺘﺳﺍ ﺎﻳ ﺩﻭﺪﻧﺍ
ﺭﺩ ﻩﺪﺷ
ﻱﺩ ﻂﺳﻮﺗ ﺖﻴﻠﭘ ﻞﻴﻨﻓﻭﺮﺘﻴﻧ
ﻦﻳﺯﺍﺭﺪﻴﻫ (DNP) ﻖﺘﺸﻣ ﻱﺮﻴﮔ ﻩﺪﺷ ﺭﺩ ﻭ
ﺪﻌﺑ ﻪﻠﺣﺮﻣ
، ﺖﺴﺷ ﺯﺍ ﺲﭘ ﻭ
ﻑﺬﺣ ﻭ ﻮﺷ ﻲﺘﻧﺁ ،ﺩﺍﺯﺁ DNP
ﻱﺩﺎﺑ
ﻲﺷﻮﮔﺮﺧ (IgG) ﻪﻴﻠﻋ DNP ) Cell Biolabs Inc ﺮﻣﺁ ؛
ﻳ ﺎﮑ (
ﺪﺷ ﻩﺩﻭﺰﻓﺍ
؛ ﺖﻴﻠﭘ ﺮﺧﺁ ﻪﻠﺣﺮﻣ ﺭﺩ ﺲﭙﺳ ﻲﺘﻧﺁ ﺎﺑ ﺎﻫ
ﻪﻳﻮﻧﺎﺛ ﻱﺩﺎﺑ
ﺎﺑ ﻩﺪﺷﺭﺍﺪﻧﺎﺸﻧ HRP
) Cell Biolabs Inc ﺮﻣﺁ ؛
ﻳ ﺎﮑ ﻪﺑﻮﻜﻧﺍ( ﺪﻧﺪﺷ
ﺭﺩ ﺏﺬﺟ ،ﺶﻨﻛﺍﻭ ﻒﻗﻮﺗ ﺯﺍ ﺲﭘ ﻪﻛ ﺮﺘﻣﻮﻧﺎﻧ٤٥٠
ﺭﺍﺪﻘﻣ ﻭ ﺖﺋﺍﺮﻗ
ﻪﻧﻮﻤﻧ ﻞﻴﻧﻮﺑﺮﻛ ﻦﻴﻴﻌﺗ ﺩﺭﺍﺪﻧﺎﺘﺳﺍ ﻲﻨﺤﻨﻣ ﺯﺍ ﻩﺩﺎﻔﺘﺳﺍ ﺎﺑ ﺎﻫ
ﺷ ﺪ ] ٦ [.
ﻩﺯﺍﺪﻧﺍ ﻱﺍﺮﺑ ﺯﺍ ﻱﺪﻴﭙﻴﻟ ﻥﻮﻴﺳﺍﺪﻴﺴﻛﺍﺮﭘ ﻱﺮﻴﮔ
ﺵﻭﺭ ﺶﻨﻛﺍﻭ
ﻚﻳﺭﻮﺘﻴﺑﺭﺎﺑﻮﻴﺗ ﻞﻣﺎﺷ ﺶﻨﮐﺍﻭ ﻁﻮﻠﺨﻣ .ﺪﺷ ﻩﺩﺎﻔﺘﺳﺍ ﺪﻴﺳﺍ
١ / ﻲﻠﻴﻣ٠ ﺮﺘﻴﻟ
،ﻡﺮﺳ ٢ / ﻲﻠﻴﻣ٠ ﻢﻳﺪﺳ ﺮﺘﻴﻟ ﻞﻴﺳﺩﻭﺩ
ﺕﺎﻔﻟﻮﺳ (SDS)
١ / ،%٨
٥ / ﻲﻠﻴﻣ١ ﮏﻴﺘﺳﺍﺪﻴﺳﺍ ﺮﺘﻴﻟ ﻭ %٢٠
٥ / ﻲﻠﻴﻣ١ ﻲﺑﺁ ﻝﻮﻠﺤﻣ ﺮﺘﻴﻟ
ﮏﻳﺭﻮﺘﻴﺑﺭﺎﺑﻮﻴﺗ ﺪﻴﺳﺍ
(TBA) ٨
/ .ﺩﻮﺑ %٠ ﺪﻴﺳﺍ pH
ﺎﺑ ﮏﻴﺘﺳﺍ
ﺮﺑﺍﺮﺑ ﹰﺎﻘﻴﻗﺩ NaOH ٥
/ ﻪﺑ ﺮﻄﻘﻣ ﺏﺁ ﺎﺑ ﻲﻳﺎﻬﻧ ﻢﺠﺣ .ﺪﺷ ﻢﻴﻈﻨﺗ ٣
ﻲﻠﻴﻣ٤ ﻭ ﺪﻴﺳﺭ ﺮﺘﻴﻟ ﺭﺩ ﻪﻘﻴﻗﺩ٦٠
٩٥°C ﺯﺍ ﺲﭘ .ﺪﺷ ﻱﺭﺍﺪﻬﮕﻧ
ﻪﻟﻮﻟ ﻥﺪﺷﺩﺮﺳ ﻲﻠﻴﻣ ﮏﻳ ،ﺏﺁ ﻑﺮﻇ ﺭﺩ ﺶﻳﺎﻣﺯﺁ ﻱﺎﻫ
ﻭ ﺮﻄﻘﻣ ﺏﺁ ﺮﺘﻴﻟ
ﻲﻠﻴﻣ ﺞﻨﭘ ﻪﻓﺎﺿﺍ ﻝﺎﻣﺮﻧ ﻞﻧﺎﺗﻮﺑ ﺮﺘﻴﻟ ﺪﺷ
ﻡﺎﺠﻧﺍ ﺐﺳﺎﻨﻣ ﻝﺎﻌﻔﻧﺍ ﻭ ﻞﻌﻓ ﺎﺗ ﻔﻳﺮﺘﻧﺎﺳ ﺯﺍ ﺲﭘ .ﺩﻮﺷ
ﻴ ﺕﺪﻣ ﻪﺑ ﮊﻮ ﺭﺩ ﻪﻘﻴﻗﺩ١٠
ﻪﻘﻴﻗﺩ ﺭﺩ ﺭﻭﺩ ٤٠٠٠ ،
ﺭﺩ (ﻻﺎﺑ ﻪﻳﻻ) ﻲﻟﺁ ﻪﻳﻻ ﺏﺬﺟ ﺮﺘﻣﻮﻧﺎﻧ٥٣٢
ﺖﻈﻠﻏ ﺯﺍ .ﺪﺷ ﻩﺪﻧﺍﻮﺧ ﻱﺎﻫ
،٥ ،١٠ ،٢٥ ،٥٠ ﻭ ٧٥ ﻩﺩﺎﻣ ﺭﻻﻮﻣﻭﺮﻜﻴﻣ١٠٠ ٣،٣،١،١
-
ﻲﺴﮐﻮﺘﻣﺍﺮﺘﺗ ﻥﺎﭘﻭﺮﭘ
(TMP) ﻪﺑ ﻩﺩﺎﻔﺘﺳﺍ ﺩﺭﺍﺪﻧﺎﺘﺳﺍ ﻥﺍﻮﻨﻋ ﺷ
ﺪ ] ٦ [ .
ﻩﺯﺍﺪﻧﺍ ﻲﺘﻧﺁ ﻱﺮﻴﮔ ﺍ ﻩﺩﺎﻔﺘﺳﺍ ﺎﺑ ﻡﺮﺳ ﻡﺎﺗ ﻥﺍﺪﻴﺴﻛﺍ
ﺖﻴﻛ ﺯ ﺍﺰﻳﻻﺍ
) Cayman ﻩﺪﺤﺘﻣ ﺕﻻﺎﻳﺍ ؛
ﺑ ( ﻪ ﻥﻮﻴﺳﺍﺪﻴﺴﻛﺍ ﺯﺍ ﺖﻌﻧﺎﻤﻣ ﻪﻠﻴﺳﻭ
ﺐﻴﻛﺮﺗ ﺪﺷ ﻡﺎﺠﻧﺍABTS
] ٦ [.
ﻪﻧﻮﻤﻧ ﻡﺎﺗ ﺪﻴﺴﻛﺍﺮﭘ ﺖﻈﻠﻏ ﺵﻭﺭ ﺎﺑ ﻲﻳﺎﻤﺳﻼﭘ ﻱﺎﻫ
ﻦﻴﻴﻌﺗ FOX2
]ﺪﺷ ٦ ﺱﺎﺳﺍ [.
ﺶﻳﺎﻣﺯﺁ
، ﻉﺍﻮﻧﺍ ﻂﺳﻮﺗ ﻚﻳﺮﻓ ﻪﺑ ﻭﺮﻓ ﻥﻮﻳ ﻥﻮﻴﺳﺍﺪﻴﺴﻛﺍ
ﻪﻧﻮﻤﻧ ﺭﺩ ﺩﻮﺟﻮﻣ ﻱﺎﻫﺪﻴﺴﻛﺍﺮﭘ ﺯﺍ ﻲﻔﻠﺘﺨﻣ ﻲﻳﺎﻤﺳﻼﭘ ﻱﺎﻫ
ﺳﺍ ﺖ ﻥﻮﻳ .
ﺞﻧﺭﻭﺍ ﻝﻮﻨﻠﻳﺰﮔ ﻲﮕﻧﺭ ﺲﻜﻠﭙﻤﻛ ﻚﻳﺮﻓ ﻲﻣ ﻞﻴﻜﺸﺗ ﻚﻳﺮﻓ-
ﻪﻛ ﺪﻫﺩ
ﻲﻣ ﺭﺩ ﺍﺭ ﻥﺁ ﺏﺬﺟ ﻥﺍﻮﺗ ﺮﺘﻣﻮﻧﺎﻧ٥٦٠
ﻩﺯﺍﺪﻧﺍ ﻪﻴﻬﺗ ﻱﺍﺮﺑ .ﺩﺮﻛ ﻱﺮﻴﮔ
ﻑﺮﻌﻣ ﺍﺪﺘﺑﺍ FOX2
٨ / ﯽﻠﻴﻣ٩ ﻡﺮﮔ ﺭﺩ ﺕﺎﻔﻟﻮﺳﻭﺮﻓ ﻡﻮﻴﻧﻮﻣﺁ
ﯽﻠﻴﻣ١٠ ﺮﺘﻴﻟ ﻚﻳﺭﻮﻔﻟﻮﺳﺪﻴﺳﺍ ﯽﻠﻴﻣ٢٥٠
ﺭﻻﻮﻣ ﻦﻳﺍ ﻪﺑ ﺲﭙﺳ ﻭ ﻞﺣ
ﻝﻮﻠﺤﻣ ﯽﻠﻴﻣ٩٠
ﺮﺘﻴﻟ ﻪﺟﺭﺩ ﺎﺑ ﻝﻮﻧﺎﺘﻣ ﻱﻭﺎﺣHPLC
٢ / ﯽﻠﻴﻣ٧٩ ﻡﺮﮔ
ﻞﻴﺗﻮﺑ ﻲﺴﻛﻭﺭﺪﻴﻫ ﺎﻬﺘﻧﺍ ﺭﺩ .ﺪﺷ ﻪﻓﺎﺿﺍ ﻦﺋﻮﻟﻮﺗ
، ٦ / ﯽﻠﻴﻣ٧ ﻡﺮﮔ ﻝﻮﻨﻠﻳﺰﮔ
ﻢﻫ ﺎﺑ ﺞﻧﺭﻭﺍ .ﺪﺷ ﻪﻓﺎﺿﺍ ﻥﺩﺯ
ﺎﺑ ﻪﻧﻮﻤﻧ ﺮﺘﻴﻟﻭﺮﻜﻴﻣ٢٠٠ ﺮﺘﻴﻟﻭﺮﻜﻴﻣ١٨٠٠
ﻑﺮﻌﻣ ﻁﻮﻠﺨﻣ FOX2
ﻭ ﻪﺑﻮﻜﻧﺍ ﺯﺍ ﺲﭘ ﻪﺑ ﻥﺪﺷ
ﺕﺪﻣ ﺭﺩ ﻪﻘﻴﻗﺩ٣٠
ﻕﺎﺗﺍ ﻱﺎﻣﺩ
، ﻪﻧﻮﻤﻧ ﺕﺪﻣ ﻪﺑ ﺎﻫ ﺭﺩ ﻪﻘﻴﻗﺩ١٠
١٢٠٠٠ ﻪﻘﻴﻗﺩ ﺭﺩ ﺭﻭﺩ
ﻔﻳﺮﺘﻧﺎﺳ ﻴ ﺭﺩ ﻲﻧﺎﻗﻮﻓ ﻝﻮﻠﺤﻣ ﺏﺬﺟ .ﺪﻧﺪﺷ ﮊﻮ ﺮﺘﻣﻮﻧﺎﻧ٥٦٠
ﻩﺯﺍﺪﻧﺍ ﻱﺮﻴﮔ
ﺖﻈﻠﻏ ﺯﺍ .ﺪﺷ ﻝﻮﻠﺤﻣ ﻒﻠﺘﺨﻣ ﻱﺎﻫ
H2O2 ﻪﺑ ﻩﺩﺎﻔﺘﺳﺍ ﺩﺭﺍﺪﻧﺎﺘﺳﺍ ﻥﺍﻮﻨﻋ
ﻝﻮﻣﻭﺮﻜﻴﻣ ﺐﺴﺣ ﺮﺑ ﺞﻳﺎﺘﻧ ﻭ H2O2
.ﺪﺷ ﺵﺭﺍﺰﮔ ﺮﺘﻴﻟ ﺭﺩ
ﻩﺩﺍﺩ ﺖﻴﻌﻤﺟ ﯼﺎﻫ ﻭ ﯽﺘﺧﺎﻨﺷ
ﺵﺯﻮﻣﺁ ﺱﺎﻨﺷﺭﺎﮐ ﻂﺳﻮﺗ ﻲﺼﺼﺨﺗ ﻩﺪﻳﺩ
ﺪﺣﺍﻭ ﺖﺒﺛ ﺷ ﻩﻭﺮﮔ ﻦﻴﺑ ﺕﻭﺎﻔﺗ .ﺪ ﺎﺑ ﺎﻫ
ﻥﻮﻣﺯﺁ ﺯﺍ ﻩﺩﺎﻔﺘﺳﺍ ﯼﺭﺎﻣﺁ ﯼﺎﻫ
ﺖﻧﺩﻮﺘﺳﺍT ﺍﺮﺑ
ﯼ ﺳﺭﺮﺑ ﯽ ﻣ ﺕﻭﺎﻔﺗ ﻴ ﮕﻧﺎ ﻴ ﻦ ﺎﻫﺮﺘﻣﺍﺭﺎﭘ ﺭﺩ ﯼ
ﻩﺯﺍﺪﻧﺍ ﮔ ﻴ ﺮ ﯼ ﺑ ﻩﺪﺷ ﻴ ﻦ ﺑ ﻭ ﻝﺮﺘﻨﮐ ﻩﻭﺮﮔ ﻴ
ﺭﺎﻤ ﻪﺑ ﻡﺮﻧ ﻚﻤﻛ ﺭﺍﺰﻓﺍ SPSS
.ﺪﺷ ﺶﺠﻨﺳ18
ﻪﺘﻓﺎﻳ ﺎﻫ
ﯽﻨﻌﻣ ﻑﻼﺘﺧﺍ ﺭﺩ ﯼﺭﺍﺩ
ﺱﺮﺘﺳﺍ ﺺﺧﺎﺷ ﻭ ﻞﻴﻧﻮﺑﺮﻛ ﻦﻴﺌﺗﻭﺮﭘ ﻥﺍﺰﻴﻣ
ﺴﻛﺍ ﯽﺸﻳﺎ ﻲﺘﻧﺁ ﺖﺒﺴﻧ) ﺪﻴﺴﮐﺍﺮﭘ ﻪﺑ ﻡﺎﺗ ﻥﺍﺪﻴﺴﮐﺍ
(ﻡﺎﺗ ﻩﻭﺮﮔ ﻭﺩ ﻦﻴﺑ
ﺭﺎﻤﻴﺑ ﻭ ﻝﺮﺘﻨﮐ
،ﺪﺸﻧ ﻩﺪﻫﺎﺸﻣ ﻲﻟﺎﺣﺭﺩ
ﻪﮐ ﻥﺍﺰﻴﻣ ﻡﺎﺗ ﺪﻴﺴﻛﺍﺮﭘ ﻩﻭﺮﮔ
،ﺭﺎﻤﻴﺑ ﺑ ﻪ ﻲﻨﻌﻣ ﺭﻮﻃ ﻱﺭﺍﺩ ) ٠٠١ / ٠ p<
ﺮﺘﻤﮐ ( ﻝﺮﺘﻨﻛ ﻩﻭﺮﮔ ﺯﺍ ﺩﻮﺑ
ﻝﻭﺪﺟ) ١ .(
ﯽﻨﻌﻣ ﯽﮕﺘﺴﺒﻤﻫ ﭻﻴﻫ ﺺﺧﺎﺷ ﻭ ﻦﺳ ﻦﻴﺑ ﯼﺭﺍﺩ
ﺱﺮﺘﺳﺍ ﯼﺎﻫ
) ﺪﺸﻧ ﻩﺪﻫﺎﺸﻣ ﻩﻭﺮﮔ ﻭﺩ ﺮﻫ ﺭﺩ ﯽﺸﻳﺎﺴﮐﺍ ۰۵
/ ۰ p>
ﻲﮕﺘﺴﺒﻤﻫ .(
ﻦﻴﺑ
ﻥﺍﺰﻴﻣ ﻭ ﻦﺳ ﯽﺘﻧﺁ
ﻝﺮﺘﻨﮐ ﻩﻭﺮﮔ ﺭﺩ ﻡﺎﺗ ﻥﺍﺪﻴﺴﮐﺍ )ﻲﻔﻨﻣ
٠٤٦ / ٠ -
= ؛r
٧٤٤ / ٠
= ) ﺖﺒﺜﻣ ﺭﺎﻤﻴﺑ ﻩﻭﺮﮔ ﺭﺩ ﻭ (p ٠٤٩
/ ٠
= ؛r ٤٥١ / ٠
= .ﺩﻮﺑ (p
ﻝﺮﺘﻨﮐ ﻩﻭﺮﮔ ﺮﻫ ﺭﺩ ﻡﺎﺗ ﺪﻴﺴﮐﺍﺮﭘ ﻥﺍﺰﻴﻣ ﻭ ﻦﺳ ﻦﻴﺑ ﯽﮕﺘﺴﺒﻤﻫ ) ١٦٧ / ٠ -
= ؛r ٢٣٦ / ٠
= ) ﺭﺎﻤﻴﺑ ﻭ (p ٠٨٤ / ٠ -
= ؛r ٢٠٢ / ٠
= ﺩﻮﺑ ﯽﻔﻨﻣ (p .
ﻝﺮﺘﻨﮐ ﻩﻭﺮﮔ ﺭﺩ ﯽﺸﻳﺎﺴﮐﺍ ﺱﺮﺘﺳﺍ ﺺﺧﺎﺷ ﻭ ﻦﺳ ﻦﻴﺑ ﯽﮕﺘﺴﺒﻤﻫ
۱۸۶ ﻥﺍﺭﺎﮑﻤﻫ ﻭ ﺵﻭﺮﺳ ﺎﺿﺭﺪﻤﺤﻣ ـــــ
ـــــــــــــــــــــــــ ــــــ
ـ ـــــــــــــــــــــــــــــــــــــــــــــــــــــــــ
ﻲﻔﻨﻣ ) ٠٧٠ / ٠
= ؛r ٦٣٥ / ٠
= (p ﺖﺒﺜﻣ ﺭﺎﻤﻴﺑ ﻩﻭﺮﮔ ﺭﺩ ﻭ )
٠٧٤ / ٠ -
= ؛r
٢٧٨ / ٠
= (p .ﺩﻮﺑ
ﻝﻭﺪﺟ ١ ( ﻥﻮﻟﺎﻣ ،ﻞﻴﻧﻮﺑﺮﻛ ﻦﻴﺌﺗﻭﺮﭘ ﻲﺳﺭﺮﺑ ﺯﺍ ﻞﺻﺎﺣ ﺞﻳﺎﺘﻧ ﻱﺩ
ﺪﻴﻫﺪﻟﺁ ﺪﻴﭙﻴﻟ)
ﻲﺘﻧﺁ ،(ﻥﻮﻴﺳﺍﺪﻴﺴﻛﺍﺮﭘ ﺴﻛﺍ ﺱﺮﺘﺳﺍ ﺺﺧﺎﺷ ﻭ ﻡﺎﺗ ﺪﻴﺴﻛﺍﺮﭘ ،ﻡﺎﺗ ﻥﺍﺪﻴﺴﻛﺍ
ﯽﺸﻳﺎ ﻦﻴﺑ
ﻩﻭﺮﮔ ﻪﻌﻟﺎﻄﻣ ﺩﺭﻮﻣ ﻱﺎﻫ
ﻩﻭﺮﮔ ﻦﻴﮕﻧﺎﻴﻣ
ﺢﻄﺳ ﯽﻨﻌﻣ ﯼﺭﺍﺩ
ﻞﻴﻧﻮﺑﺮﻛ ﻦﻴﺌﺗﻭﺮﭘ ﯽﻠﻴﻣ ﺮﺑ ﻝﻮﻣﻮﻧﺎﻧ)
(ﻦﻴﺌﺗﻭﺮﭘ ﻡﺮﮔ
ﻝﺮﺘﻨﮐ ۷۲
/ ۱
± ۲۱ / ٣٤١ ۳ /
٠ ۳/۴۲±۱/۴۴ ﺭﺎﻤﻴﺑ
ﻥﻮﻟﺎﻣ ﻱﺩ ﺪﻴﻫﺪﻟﺁ (ﻝﻮﻣﻭﺮﮑﻴﻣ)
ﻝﺮﺘﻨﮐ ۶۸
/ ۱
± ۵۵ / ٠٩٥ ۵ /
٠ ۵/۱۷±۱/۶۰ ﺭﺎﻤﻴﺑ
ﻲﺘﻧﺁ ﻡﺎﺗ ﻥﺍﺪﻴﺴﻛﺍ ﯽﻠﻴﻣ)
(ﻝﻮﻣ
ﻝﺮﺘﻨﮐ ۴۲
/ ۱
± ۷۴ / ٤٧٣ ۱ /
٠ ۱/۸۹±۱/۴۷ ﺭﺎﻤﻴﺑ
ﻡﺎﺗ ﺪﻴﺴﻛﺍﺮﭘ ﻝﻮﻣﻭﺮﮑﻴﻣ)
H2O2
ﺮﺘﻴﻟ ﺮﺑ (
ﻝﺮﺘﻨﮐ ۷۸
/ ۳
± ۷۶ / ٠٠١ ۱۳ /
<٠
ﺭﺎﻤﻴﺑ ۱۵
/ ۷
± ۰۹ / ۱۷
ﺴﻛﺍ ﺱﺮﺘﺳﺍ ﺺﺧﺎﺷ ﯽﺸﻳﺎ
ﻝﺮﺘﻨﮐ ۶۹
/ ۱
± ۱۶ / ۵۷۷ ۱ /
۰ ۱/۴۴±۲/۳۱ ﺭﺎﻤﻴﺑ
ﺚﺤﺑ
ﻲﻳﺎﻴﻤﻴﺷ ﻱﺎﻫﺯﺎﮔ ﺯﺍ ﻥﮊﻮﻧﺎﻴﺳ ﺯﺎﮔ ﻭ ﺏﺎﺼﻋﺍ ﺯﺎﮔ ،ﻱﺩﺮﮔﻮﮔ ﻝﺩﺮﺧ ﺯﺎﮔ ﻲﮕﻨﺟ ﺪﻨﺘﺴﻫ ﻣﻭﺪﺼﻣ . ﺎ ﻥ ﺽﺭﺍﻮﻋ ﺯﺍ ﻩﺯﻭﺮﻣﺍ ﻪﻛ ﻥﺍﺮﻳﺍ ﺭﺩ ﻲﻳﺎﻴﻤﻴﺷ
ﻲﻣ ﺞﻧﺭ ﻲﻳﺎﻴﻤﻴﺷ ﻞﻣﺍﻮﻋ ﺱﺭﺮﻳﺩ ﻱﺩﺮﮔﻮﮔ ﻝﺩﺮﺧ ﻥﺎﻴﻧﺎﺑﺮﻗ ﹰﺎﺗﺪﻤﻋ ﺪﻧﺮﺑ
ﺍﺮﭼ ،ﺪﻨﺘﺴﻫ ﺕﻮﻓ ﺎﻳ ﺏﺎﺼﻋﺍ ﻞﻣﺍﻮﻋ ﻭ ﻥﮊﻮﻧﺎﻴﺳ ﻥﺎﻴﻧﺎﺑﺮﻗ ﻩﺪﻤﻋ ﻪﻛ
ﻥﺎﻣﺭﺩ ﺯﺍ ﺪﻌﺑ ﺎﻳ ﻩﺪﺷ ﻪﺘﻓﺎﻳ ﺩﻮﺒﻬﺑ ﻪﻴﻟﻭﺍ ﻱﺎﻫ
ﺪﻧﺍ ﻥﺎﻣﺯﺎﺳ ﺵﺭﺍﺰﮔ ﻖﺒﻃ.
ﺖﺷﺍﺪﻬﺑ
،ﯽﻧﺎﻬﺟ ﻼﻋ ﻳ ﺎﺑ ﺩﺎﺣ ﻪﻬﺟﺍﻮﻣ ﻢ ﻞﻣﺎﺷ ﻲﻳﺎﻴﻤﻴﺷ ﻱﺎﻫﺯﺎﮔ
،ﻪﺴﻄﻋ ﺭﺎﺸﻓ ﺱﺎﺴﺣﺍ ،ﻪﻓﺮﺳ ،ﻲﻨﻴﺑ ﻱﺰﻳﺮﻧﻮﺧ ،ﻚﺷﺍ ،ﻲﻨﻴﺑ ﺵﺰﻳﺮﺑﺁ
ﺮﺑ ﺴﻔﻗ ﻪ ﻲﻛﺎﺗ ﻭ ﻢﻠﻜﺗ ﻝﻼﺘﺧﺍ ،ﻪﻨﻴﺳ ﺖﺳﺍ ﻪﻨﭘ
] ٧ ﹰﺎﺒﻳﺮﻘﺗ ﺯﺍ ﺪﻌﺑ .[ ﻭﺩ
ﻝﺎﺳ
، ﺖﻴﻣﻭﺪﺼﻣ ﻦﻳﺍ ﺯﺍ ﻲﺷﺎﻧ ﺱﺭﺮﻳﺩ ﺽﺭﺍﻮﻋ ﺎﻫ
ﻪﺑ ﺕﻻﻼﺘﺧﺍ ﺕﺭﻮﺻ
،ﻱﻮﻳﺭ ﻲﻣ ﺮﻫﺎﻈﺗ ﻱﺰﮐﺮﻣ ﺏﺎﺼﻋﺍ ﻢﺘﺴﻴﺳ ﻭ ﻲﺘﺳﻮﭘ ،ﻲﻤﺸﭼ ﺪﻳﺎﻤﻧ
] ٨ [.
ﻞﻣﺎﺷ ﻪﻛ ﺖﻴﻣﻭﺪﺼﻣ ﺕﺪﻣﺯﺍﺭﺩ ﺽﺭﺍﻮﻋ ﻒﻠﺘﺨﻣ ﺕﺎﻌﻟﺎﻄﻣ ﺭﺩ
ﺍ ﻪﺳ ﻪﻛ ﻱﻮﻳﺭ ﻭ ﻲﺘﺳﻮﭘ ،ﻲﻤﺸﭼ ﺕﻼﻜﺸﻣ ﻡﺍﺪﻧ
ﺭﺩ ﻑﺪﻫ ﻩﺪﻤﻋ
ﺪﻨﺘﺴﻫ ﺖﻴﺣﻭﺮﺠﻣ ﻦﻣﺰﻣ ﻪﻠﺣﺮﻣ
، ﺖﺳﺍ ﻪﺘﻓﺮﮔ ﺭﺍﺮﻗ ﻲﺳﺭﺮﺑ ﺩﺭﻮﻣ ]
٧ [ .
ﻱﺮﻃﺎﺧ ﻥﺍﺭﺎﮑﻤﻫ ﻭ ﻪﻌﻟﺎﻄﻣ ﺎﺑ
ﺭﺍﺰﻫ٣٤ ﻝﺩﺮﺧ ﺯﺎﮔ ﻲﻳﺎﻴﻤﻴﺷ ﻡﻭﺪﺼﻣ
،
١٤٤٥٠ ﺩﺭﻮﻣ ) ٥ / ﻌﻳﺎﺿ (%٤٢ ﻪ ﻱﻮﻳﺭ
، ١٣٣٦٢ ﺩﺭﻮﻣ ) ٣ / ﻌﻳﺎﺿ (%٣٩ ﻪ
ﻭ ﻲﻤﺸﭼ ﺩﺭﻮﻣ ٨٣٣٨
) ١ / ﻌﻳﺎﺿ (%٢٤ ﻪ ﻲﺘﺳﻮﭘ ﻩﺩﺮﮐ ﺵﺭﺍﺰﮔ .ﺪﻧﺍ
ﺕﺍﺮﻴﻴﻐﺗ ﺱﺎﺳﺍﺮﺑ ﺩﺍﺮﻓﺍ ﻦﻳﺍ ﻱﻮﻳﺭ ﻪﻌﻳﺎﺿ ﻪﺘﻓﺎﻳ ﻭ PFT
ﻪﻨﻳﺎﻌﻣ ﻱﺎﻫ ﺘﺳﺩ ﺭﺎﻬﭼ ﻪﺑ ﻲﻜﻳﺰﻴﻓ
ﻪ ﻢﻴﺴﻘﺗ ﺪﻳﺪﺷ ﻭ ﻂﺳﻮﺘﻣ ،ﻒﻴﻔﺧ ،ﻲﻌﻴﺒﻃ ﻱﺪﻨﺑ
ﺪﺷ ﻪﻛ ،ﻒﻴﻔﺧ ﻱﺮﻴﮔﺭﺩ ﺩﺍﺮﻓﺍ ﻦﻳﺍ %٧٥ ﻭ ﻂﺳﻮﺘﻣ ﻱﺮﻴﮔﺭﺩ %١٥
%١٠
ﺪﻨﺘﺷﺍﺩ ﻱﻮﻳﺭ ﺪﻳﺪﺷ ﻱﺮﻴﮔﺭﺩ ]
١ ﺮﺿﺎﺣ ﻪﻌﻟﺎﻄﻣ ﺭﺩ [.
، ﺎﻬﻨﺗ ﺶﻫﺎﮐ
ﻣﻭﺪﺼﻣ ﺭﺩ ﻡﺎﺗ ﺪﻴﺴﮐﺍﺮﭘ ﺎ
ﻨﻌﻣ ﻥ ﯽ ﺭﺍﺩ ﺩﻮﺑ ﻲﻨﻌﻣ ﺶﻳﺍﺰﻓﺍ . ﻥﺍﺰﻴﻣ ﺭﺩ ﺭﺍﺩ
ﺭﺩ ﺯﻻﺎﺗﺎﮐ ﻢﻳﺰﻧﺁ ﺖﻴﻟﺎﻌﻓ ﺶﻫﺎﮐ ﻭ ﺯﺎﺗﻮﻤﺴﻳﺩ ﺪﻴﺴﮐﺍ ﺮﭘﻮﺳ ﻢﻳﺰﻧﺁ ﺭﺍﺰﮔ ﻝﺩﺮﺧ ﺯﺎﮔ ﺎﺑ ﻱﻮﻳﺭ ﻦﻴﻣﻭﺪﺼﻣ ﺖﺳﺍ ﻩﺪﺷ ﺵ
] ﺭﺩ ﺭﻮﻄﻨﻴﻤﻫ .[۱۰
ﻌﻟﺎﻄﻣ ﻪ ﺶﻫﺎﮐ ،ﻱﻮﻳﺭ ﺪﻳﺪﺷ ﻥﺎﻣﻭﺪﺼﻣ ﺭﺩ ﻥﺍﺭﺎﮑﻤﻫ ﻭ ﻲﺗﻭﺍﺮﻃ
ﻨﻌﻣ ﯽ ﺭﺍﺩ ﻢﻳﺰﻧﺁ ﺖﻴﻟﺎﻌﻓ ﺭﺩ ﻥﻮﻴﺗﺎﺗﻮﻠﮔ ﻱﺎﻫ
ﺭﺩ ﺯﺎﻧﻮﺴﮐﺍﺭﺎﭘ ﻭ ﺯﺍﺮﻔﺴﻧﺍﺮﺗ
ﺼﻣ ﻦﻴﻣﻭﺪ ﭗﻴﺗﻮﻧﮊ ﺭﺩ ﺶﻬﺟ ﻭ ﻝﺮﺘﻨﮐ ﻩﻭﺮﮔ ﻪﺑ ﺖﺒﺴﻧ ﻢﻳﺰﻧﺁ R
] ﺖﺳﺍ ﻩﺪﺷ ﺵﺭﺍﺰﮔ ﺯﺎﻧﻮﺴﮐﺍﺭﺎﭘ ،۱۱
۱۲ .[
ﻪﻧﻮﮔ ﺮﻫ ﺮﻤﻋ ﻝﻮﻃ ﻩﺪﻧﺯ
، ﻝﻮﻠﺳ ﺖﻣﻭﺎﻘﻣ ﺎﺑ ﻲﺘﺒﺜﻣ ﻪﻄﺑﺍﺭ ﯼﺎﻫ
ﻪﺑ ﻥﺁ
ﺱﺮﺘﺳﺍ ﺩﺭﺍﺩ ] ١٢ ﻝﻮﻜﻟﻮﻣﻭﺮﻛﺎﻣ ﻊﻤﺠﺗ ،ﻥﺪﺷﺮﻴﭘ ﻲﻃ [.
ﻱﺎﻫ
ﺎﺑ ﻪﺘﻓﺎﻳﺮﻴﻴﻐﺗ ﺢﻄﺳ ﺭﺩ ﻲﺘﺣ ،ROS
ﺢﻄﺳ ﺶﻳﺍﺰﻓﺍ ﺐﺟﻮﻣ ،DNA
ﻲﺘﻧﺁﻮﺗﺍ ﻪﻴﻠﻋ ﻱﺩﺎﺑ ﺎﺑ ﻪﺘﻓﺎﻳﺮﻴﻴﻐﺗDNA
ﺎﻫROS ﻲﻣ ﺩﻮﺷ ] ١٣ ﺢﻄﺳ .[
ﻦﻴﺌﺗﻭﺮﭘ ﻲﻣ ﺶﻳﺍﺰﻓﺍ ﻦﺳ ﺎﺑ ﻪﺘﻓﺎﻳﺮﻴﻴﻐﺗ ﻱﺎﻫ ﺭﺩ ﻝﺎﺜﻣ ﻱﺍﺮﺑ ،ﺪﺑﺎﻳ
ﺍ ﺖﻴﺳﻭﺮﺘﻳﺭ ﺖﺳﺩﺯﺍ ﺎﺑ ﺮﻴﻴﻐﺗ ﻦﻳﺍ ﻥﺎﺴﻧﺍ ﻥﻮﺧ ﺵﺩﺮﮔ ﻱﺎﻫ ﺖﻴﻟﺎﻌﻓ ﻥﺩﺍﺩ
ﺩﺭﺍﺩ ﺖﻘﺑﺎﻄﻣ ﺮﻛﺭﺎﻣ ﻢﻳﺰﻧﺁ ]
١٤ ﺍﺮﺤﺻ ﺵﻮﻣ ﻝﺪﻣ ﺭﺩ .[
ﻳ ﻲ
، ﺶﻳﺍﺰﻓﺍ ﺎﺑ
ﻦﻴﺌﺗﻭﺮﭘ ﻊﻤﺠﺗ ﻦﺳ ﺎﺑ ﻩﺍﺮﻤﻫ ﺰﻐﻣ ﻭ ﺪﺒﻛ ﺖﻓﺎﺑ ﺭﺩ ﻩﺪﺷﺪﻴﺴﻛﺍ ﻱﺎﻫ
ﺖﺳﺩﺯﺍ ﻦﻴﻣﺎﺗﻮﻠﮔ ﺖﻴﻟﺎﻌﻓ ﻥﺩﺍﺩ ﺰﻛﻮﻠﮔ ﻭ ﺯﺎﺘﺘﻨﺳ
- ﺕﺎﻔﺴﻓ٦ ﺍﺭ ﺯﺎﻧﮊﻭﺭﺪﻴﻫﺩ
ﻲﻣ ﺩﻮﻤﻧ ﻩﺪﻫﺎﺸﻣ ﻥﺍﻮﺗ ﻞﺧﺍﺩ ﻖﻳﺭﺰﺗ) ﺮﻴﭘ ﺕﺎﻧﺍﻮﻴﺣ (ﻦﻣﺰﻣ) ﺪﻨﻛ ﻥﺎﻣﺭﺩ .
ﺎﺑ (ﻲﻗﺎﻔﺻ -N
ﻞﻴﺗﻮﺑ - -α ﻞﻴﻨﻓ ﻥﻭﺮﺘﻴﻧ ﻡﺍﺩ ﻪﺑ ﺍﺭ ﺩﺍﺯﺁ ﻝﺎﻜﻳﺩﺍﺭ ﻪﻛ
ﻲﻣ ﺩﺯﺍﺪﻧﺍ
، ﻲﻌﻴﺒﻃ ﻪﺑ ﺮﺠﻨﻣ ﻱﺩﺎﻳﺯ ﻲﻳﺎﻴﻤﻴﺷﻮﻴﺑ ﻱﺎﻫﺮﺘﻣﺍﺭﺎﭘ ﻥﺪﺷ
ﻲﻣ ﺩﻮﺷ ﻲﻣ ﺍﺪﻴﭘ ﺍﺭ ﻥﺍﻮﺟ ﺕﺎﻧﺍﻮﻴﺣ ﺕﺎﺼﺨﺸﻣ ) ﻨﻛ
ﻨ ﻭ ﺪ ﺺﺧﺎﺷ ﻩﺮﻃﺎﺧ
ﻩﺎﺗﻮﻛ ﻣ ﺩﻮﺒﻬﺑ ﻥﺍﻮﺟ ﺕﺎﻧﺍﻮﻴﺣ ﺪﺣ ﺭﺩ ﺎﻬﻧﺁ ﺕﺪﻣ ﻲ
ﺪﺑﺎﻳ ﺴﻣ ﻦﻳﺍ .( ﺎ ﻪﻟ
ﻢﻳﺰﻧﺁ ﻦﺳ ﻪﺑ ﻪﺘﺴﺑﺍﻭ ﻊﻤﺠﺗ ﻦﻴﺑ ﻁﺎﺒﺗﺭﺍ
ﺖﺳﺩﺯﺍ ﻭ ﻩﺪﺷﺪﻴﺴﻛﺍ ﻱﺎﻫ ﻦﺘﻓﺭ
ﻲﻣ ﻥﺎﺸﻧ ﺍﺭ ﻚﻳﮊﻮﻟﻮﻳﺰﻴﻓ ﺩﺮﻜﻠﻤﻋ ﺪﻫﺩ
] ١٤ ﻪﮑﻨﻳﺍ ﺎﺑ.[
ﻦﻳﺍ ﻪﻳﺮﻈﻧ ﺩﺭﻮﻣ
ﺑ ﻝﻮﺒﻗ ﻪ ﻲﻣ ﺮﻈﻧ ﺪﺳﺭ
، ﺎﺗ ﺎﻣﺍ ﻳ ﻝﺪﻣ ﺯﺍ ﻩﺩﺎﻔﺘﺳﺍ ﺎﺑ .ﺖﺳﺍ ﻩﺪﺸﻧ ﺪﻴ ﻱﺎﻫ
ﺩﺍﺩ ﻥﺎﺸﻧ ﻩﻮﻴﻣ ﺲﮕﻣ ﻭ ﺖﺨﻳﺭﺍﺮﺗ ﻲﺷﻮﻣ ﻩﺪﺷ ﻩ
ﺡﻼﺻﺍ ﻪﻛ
ﻴﻧﺎﻜﻣ ﺰ ﻡ ﻲﺘﻧﺁ ﻉﺎﻓﺩ ﻱﺎﻫ ﻞﺜﻣ ﻲﻧﺍﺪﻴﺴﻛﺍ
ﺯﺎﺗﻮﻤﺴﻳﺩ ﺪﻴﺴﮐﺍﺮﭘﻮﺳ
(SOD) ﻲﻣ ﻩﺩﺮﻛ ﺮﺛﺍ ﻱﺮﻴﭘ ﺪﻧﻭﺭ ﺭﺩ ﺪﻧﺍﻮﺗ ﻭ
ﺪﻫﺩ ﺮﻴﻴﻐﺗ ﺍﺭ ﺮﻤﻋ ﻝﻮﻃ
] ١٥ ﻲﻟﺎﺣﺭﺩ ،[ ﻪﻛ ﻑﺮﺼﻣ ﻲﺘﻧﺁ ﻥﺍﺪﻴﺴﻛﺍ ﺮﻴﻴﻐﺗ ﺍﺭ ﺮﻤﻋ ﻝﻮﻃ ﻲﻳﺍﺬﻏ ﻱﺎﻫ
ﻲﻤﻧ ﻥﺍﺰﻴﻣ .ﺪﻫﺩ ﻝﺩﺮﺧ ﺯﺎﮔ
ﻢﻳﺰﻧﺁ ﺮﺑ ﻲﺗﻭﺎﻔﺘﻣ ﺮﺛﺍ ﻲﺘﻧﺁ ﻱﺎﻫ
ﻥﺍﺪﻴﺴﻛﺍ
ﺩﺭﺍﺩ ﻥﺍﺰﻴﻣ . ﺯﺍ ﺮﺘﻤﻛ ﻡﺮﮔﻮﻠﻴﮐ ﺮﺑ ﻡﺮﮔﻭﺮﮑﻴﻣ١٠
ﻝﺩﺮﺧ ﺯﺎﮔ ﺐﺒﺳ
ﺶﻳﺍﺰﻓﺍ ﺖﻴﻟﺎﻌﻓ ﻩﺎﺗﻮﻛ ﺕﺪﻣ ﺭﺩ) ﻲﻤﻳﺰﻧﺁ ٧
- ﻪﻠﻤﺟ ﺯﺍ (ﺯﻭﺭ٢
ﻥﻮﻴﺗﺎﺗﻮﻠﮔ -S ﺯﺍﺮﻔﺴﻧﺍﺮﺗ (GST) ﻲﻣ ﺩﻮﺷ ﺮﺘﺸﻴﺑ ﺕﺪﻣ ﺭﺩ ﻭ ﺯﺍ
ﺯﻭﺭ١٤
ﺯﺍ ﺮﺗﻻﺎﺑ ﺮﻳﺩﺎﻘﻣ ﺎﺑ ﻭ
،ﻡﺮﮔﻮﻠﻴﮐ ﺮﺑ ﻡﺮﮔﻭﺮﮑﻴﻣ١٠ ﺶﻫﺎﻛ
ﺎﻌﻓ ﻦﻳﺍ ﺖﻴﻟ
ﻲﻣ ﻩﺪﻫﺎﺸﻣ ﻢﻳﺰﻧﺁ ﺩﻮﺷ
] ١٦ .[
ﻟﺎﺑ ﺕﻼﮑﺸﻣ ﻞﻴﻟﺩ ﻪﺑ ﻴﻨ
ﯽ ﺷ ﻥﺍﺯﺎﺒﻧﺎﺟ ﻪﻌﻣﺎﺟ ﻴ
ﻤ ﻴﺎ ﻲﻳ ﻣﻭﺪﺼﻣ ﺎﺑ ﻴ ﺖ
ﺪﺷ ﻳ ﺪ ﺭ ﻳ ﻮ ﯼ ﺍﺮﺟﺍ ﻞﺤﻣ ﺭﺩ ﺭﻮﻀﺣ ﻥﺎﮑﻣﺍ ﻡﺪﻋ ﻭ ﯼ
ﺸﺨﺑ ﻪﻌﻟﺎﻄﻣ ﯽ
ﺯﺍ
ﻪﻧﻮﻤﻧ .ﺪﻧﺪﺸﻧ ﻪﻌﻟﺎﻄﻣ ﺩﺭﺍﻭ ﺎﻫ ﭘﻴ
ﺩﺎﻬﻨﺸ ﻣ ﯽ ﺩﻮﺷ ﻪﮐ ﻱﺍﺮﺑ ﻌﺗ ﻴﻴ ﻦ ﻦﺳ
ﻗﺩ ﻴ ﻖ ﺑ ﻴ ﮊﻮﻟﻮ ﻳ ﮏ ﻣﻭﺪﺼﻣ ﺭﺩ ﺎ
ﻥ ﺷ ﻴ ﻤ ﻴﺎ
،ﻲﻳ ﺎﺑ ﺎﺳ ﻳ ﺮ ﺵﻭﺭ ﻧ ﺎﻫ ﻴ ﺰ
ﺗﺎﻌﻟﺎﻄﻣ ﻲ ﺬﭘ ﺕﺭﻮﺻ ﻳ ﺩﺮ .
ـــــــــــــ ـــــــــــ
ــــ ﺺﺧﺎﺷ ﻲﮕﺘﺴﺒﻤﻫ ﺎﻫ
ﯼ ﺸﻳﺎﺴﻛﺍ ﺱﺮﺘﺳﺍ ﯽ
ﻳﺎﻴﻤﻴﺷ ﻥﺎﻣﻭﺪﺼﻣ ﺭﺩ ﻦﺳ ﺎﺑ ﯽ
ﻝﺎﺳ ؛ﺪﻳﺪﺷ ﻱﻮﻳﺭ ﻝﺩﺮﺧ ﺯﺎﮔ ﺎﺑ ﻪﻬﺟﺍﻮﻣ ﺯﺍ ﺲﭘ ﺎﻫ
۱۸۷
ﻪﺠﻴﺘﻧ ﻱﺮﻴﮔ
ﻲﻧﻻﻮﻃ ﺭﺩ ﻪﺑ ،ﺕﺪﻣ
ﺭﻮﻈﻨﻣ ﺯﺎﮔ ﻪﺑ ﻩﺪﺷﺩﺎﺠﻳﺍ ﺦﺳﺎﭘ ﻝﺮﺘﻨﮐ ﻭ ﻑﺬﺣ
ﻣﻭﺪﺼﻣ ﻥﺪﺑ ﺭﺩ ﻝﺩﺮﺧ ﺎ
ﻲﺘﻧﺁ ،ﻲﻳﺎﻴﻤﻴﺷ ﻥ ﻭ ﺶﻳﺍﺰﻓﺍ ﻡﺎﺗ ﻥﺍﺪﻴﺴﮐﺍ
ﮐ ﻡﺎﺗ ﺪﻴﺴﮐﺍﺮﭘ ﯽﻣ ﺶﻫﺎ
ﺪﺑﺎﻳ ﻭ ﺺﺧﺎﺷ ﺴﮐﺍ ﺱﺮﺘﺳﺍ ﺎﻳ
ﯽﺸ ﺮﻴﻴﻐﺗ ﻥﻭﺪﺑ
ﯽﻣ ﯽﻗﺎﺑ ﺪﻧﺎﻣ .
:ﯽﻧﺍﺩﺭﺪﻗ ﻭ ﺮﮑﺸﺗ ﺩﺍﺮﻓﺍ ﻡﺎﻤﺗ ﺯﺍ ﺡﺮﻃ ﻱﺮﺠﻣ
ﺖﮐﺮﺷ ﻦﻳﺍ ﺭﺩ ﻩﺪﻨﻨﮐ
ﻲﻣ ﻲﻧﺍﺩﺭﺪﻗ ﻭ ﺮﮑﺸﺗ ﻪﻧﺎﻤﻴﻤﺻ ﻪﻌﻟﺎﻄﻣ .ﺪﻨﮐ
:ﯽﻗﻼﺧﺍ ﻪﻳﺪﻴﻳﺎﺗ ﯽﻗﻼﺧﺍ ﺯﻮﺠﻣ
ﻕﻼﺧﺍ ﻱﺍﺭﻮﺷ ﺭﺩ ﻪﻌﻟﺎﻄﻣ ﻦﻳﺍ
ﻥﺍﺯﺎﺒﻧﺎﺟ ﻲﮑﺷﺰﭘ ﻡﻮﻠﻋ ﻭ ﻲﺳﺪﻨﻬﻣ ﻩﺪﮑﺸﻫﻭﮋﭘ .ﺖﺳﺍ ﻩﺪﻴﺳﺭ ﺪﻴﻳﺎﺗ ﻪﺑ
:ﻊﻓﺎﻨﻣ ﺽﺭﺎﻌﺗ .ﺖﺳﺍ ﻩﺪﺸﻧ ﺵﺭﺍﺰﮔ ﻥﺎﮔﺪﻨﺴﻳﻮﻧ ﻑﺮﻃ ﺯﺍ ﻱﺩﺭﻮﻣ
:ﯽﻟﺎﻣ ﻊﺑﺎﻨﻣ ﻂﺳﻮﺗ ﻪﻌﻟﺎﻄﻣ ﻦﻳﺍ
ﻡﻮﻠﻋ ﻭ ﻲﺳﺪﻨﻬﻣ ﻩﺪﮑﺸﻫﻭﮋﭘ
.ﺖﺳﺍ ﻩﺪﺷ ﻲﻟﺎﻣ ﺖﻳﺎﻤﺣ ﻥﺍﺯﺎﺒﻧﺎﺟ ﻲﮑﺷﺰﭘ
ﻊﺑﺎﻨﻣ
1- Khateri S, Ghanei M, Keshavarz S, Soroush M, Haines D.
Incidence of lung, eye, and skin lesions as late complications in 34,000 Iranians with wartime exposure to mustard agent. J Occup Environ Med. 2003;45(11):1136-43.
2- Sidell FR, Takafuji ET, Franz DR. Medical Aspects of Chemical and Biological Warfare (Textbook of Military Medicine. Part 1; Warfare, Weaponry, and the Casualty, Volume 3). 1st ed. Washington: United States Government Printing; 1997.
3- U.N. Security Council. Report of the specialists appointed by the secretory general to investigate allegations by Iran concerning the use of chemical weapons [Internet].
New York: Wisconsin Project on Nuclear Arms Control.
[Cited: March 26, 1984]. Available from:
http://www.iranwatch.org/library/international-
organization/united-nations-un/un-security-council/report- specialist-appointed-secretary-general-investigate- allegations-islamic.
4- Kumar O, Sugendran K, Vijayaraghavan R. Protective effect of various antioxidants on the toxicity of sulphur mustard administered to mice by inhalation or percutaneous routes. Chem Biol Interact. 2001;134(1):1-12.
5- Andziak B, O'Connor TP, Qi W, DeWaal EM, Pierce A, Chaudhuri AR, et al. High oxidative damage levels in the longest-living rodent, the naked mole-rat. Aging Cell.
2006;5(6):463-71.
6- Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95(2):351-8.
7- Balali-Mood M, Hefazi M. Comparison of early and late toxic effects of sulfur mustard in Iranian veterans. Basic Clin Pharmacol Toxicol. 2006;99(4):273-82.
8- Miyazawa T. Determination of phospholipid hydroperoxides in human blood plasma by a chemiluminescence-HPLC assay. Free Radic Biol Med.
1989;7(2):209-17.
9- Zarchi K, Akbar A, Naieni KH. Long term pulmonary complications in compatants exposed to mustard gas: A historical cohort study. Int J Epidemiol. 2004;33(3):579-81.
10- Shohrati M, Ghanei M, Shamspour N, Jafari M. Activity and function in lung injuries due to sulphur mustard.
Biomarkers. 2008;13(7):728-33.
11- Taravati A, Ardestani SK, Ziaee AA, Ghorbani A, Soroush MR, Faghihzadeh S, et al. Effects of paraoxonase 1 activity and gene polymorphisms on long-term pulmonary complications of sulfur mustard-exposed veterans. Int Immunopharmacol. 2013;17(3):974-9.
12- Kapahi P, Boulton ME, Kirkwood TB. Positive correlation between mammalian life span and cellular resistance to stress. Free Radic Biol Med. 1999;26(5-6):495- 500.
13- Ashok BT, Ali R. Binding of circulating antibodies to reactive oxygen species modified-DNA and detecting DNA damage by a monoclonal antibody probe. Mech Ageing Dev. 1998;103(1):69-80.
14- Stadtman ER, Starke-Reed PE, Oliver CN, Carney JM, Floyd RA. Protein modification in aging. In: Emerit I, Chance B. Free Radicals and Aging (Volume 62).
Switzerland: Birkhäuser Basel; 1992. pp. 64-72.
15- Landis GN, Tower J. Superoxide dismutase evolution and life span regulation. Mech Ageing Dev.
2005;126(3):365-79.
16- Jafari M. Dose- and time-dependent effects of sulfur mustard on antioxidant system in liver and brain of rat.
Toxicology. 2007;231(1):30-9.