The Effect of 4 Weeks Aerobic Swimming Training and Aqueous Urtica Dioica Extract on PGC- 1α and UCP-1 Gene Expression and Serum Concentrations of

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The Effect of 4 Weeks Aerobic Swimming Training and Aqueous Urtica Dioica Extract on PGC- 1α and UCP-1 Gene Expression and Serum Concentrations of

Irisin and FGF21 in Diabetic Rats

Abbas Ranjbari¹ , Khalid Mohamadzadeh Salamat²^ , Samad Akbarzadeh³ ,

Gholamreza Khamisipour⁴ , Behrouz Naeimi⁵

1. Department of Physical Education and Sport Sciences, Islamic Azad University, Sanandaj Branch, Sanandaj, Iran.

E-mail: ranjbariabas@yahoo.com

2. Corresponding Author, Department of Physical Education and Sport Sciences, Islamic Azad University, Sanandaj branch, Sanandaj, Iran E-mail: kh.mohamadzadeh@iausdj.ac.ir

3. Department of Biochemistry, Bushehr University of Medical Sciences, Bushehr, Iran E-mail: s.akbarzadeh@bpumc.ac.ir 4. Department of Laboratory Hematology and Blood Bank, Bushehr University of Medical Sciences, Bushehr, Iran.

E-mail: R.khamisipour@bpumc.ac.ir

5.Department of Medical Mycology, Bushehr University of Medical Sciences, Bushehr, Iran.

E-mail: b.naeimi@bpumc.ac.ir

Article Info ABSTRACT

Article type:

Research

Article history:

Received:

12 July 2022 Received in revised form:

17 February2023 Accepted:

21 February2023 Published online:

21 February2023

Keywords:

Aerobic Training, Diabetes, Irisin, PGC-1α, UCP-1,

Urtica Dioica extract.

Introduction: Sports activity and Urtica Dioica extract consumption increase the expression of genes involved in the conversion of white to brown adipose tissue, but their combined effect is not yet clear. This study aimed to investigate the effect of Urtica dioica extract consumption and aerobic training on serum Irisin and FGF21 concentration and PGC-1α and UCP1 gene expression in diabetic obese rats.

Methods: In an experimental design, Seventy male Wistar rats (mean weight:

227.35± 18.52 grams) were randomly divided into seven Healthy Control, Diabetic Control, Urtica Dioica, Metformin, Training, Urtica Dioica + Training, and Metformin + Training groups. The Training groups performed forced swimming training for four weeks, five days per week, and each day from 15 minutes (in the first week) to 50 minutes (in the fourth week). Each day, Urtica Dioica and Metformin groups received 1.25 and 100 mg/kg of these two substances, respectively via gavage. After the end of the intervention, rats’ blood samples, posterior thigh muscle tissues, pancreas, and fat (visceral) were taken to check the research variables. One-way analysis of variance and Bonferroni's post hoc test with a significance level of 0.05 was used to analyze the data.

Results: The serum Irisin concentration of the Metformin + exercise group was significantly higher than the Diabetic Control group (P<0.05). No significant difference was observed in the serum FGF21 concentration of all groups. A significant increase in PGC-1α and UCP-1 gene expression was observed in all treatment groups compared to Healthy Control and Diabetic Control groups (P<0.05).

Conclusion: The simultaneous consumption of Urtica Dioica extract and swimming training reduces blood glucose and also activates the PGC-1α and UCP- 1 gene expression, which play a role in adipose tissue browning.

Cite this article: Ranjbari A., Mohammadzadeh Salamat Kh., Akbarzadeh S., Khamisipour Gh., & Naeimi B. The Effect of 4 Weeks Aerobic Swimming Training and Aqueous Urtica Dioica Extract on PGC-1α and UCP-1 Gene Expression and Serum Concentrations of Irisin and FGF21 in Diabetic Rats. Journal of Sport Biosciences. 2022; 14 (4): 67-80.

DOI: https://doi.org/10.22059/jsb.2023.345715.1546

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ExtendedAbstract

Introduction

Diabetes is the most common metabolic disease in the world. Adipose tissue is known as a training endocrine organ that is active in controlling body metabolism.

There are two forms of fat in the body, white and brown fat. Brown fat has a thermogenic role and causes the conversion of chemical to thermal energy. The Urtica Dioica is used in the traditional medicine of Iran and other countries. Urtica Dioica increases insulin secretion from Langerhans beta cells and acts as a strong stimulus for the release of insulin from beta cells. Many studies have investigated the hypoglycemic effects of Urtica Dioica and also the effect of physical activity on diabetes, but until now, the simultaneous effect of physical activity and Urtica Dioica extract consumption on the genes expression and the concentration of proteins effective in converting white to brown adipose tissue in people with diabetes have not been investigated.

Methods

The present study was conducted experimentally on 70 male Wistar rats with a mean weight of 227.35±18.52 grams and an age range of 2-3 months. The rats were randomly divided into seven Healthy Control, Diabetic Control, Urtica Dioica, Metformin, Training, Urtica Dioica + Training, and Metformin + Training groups (10 rats in each group). Diabetes was induced via intraperitoneal injection of 50 mg/kg of STZ (Enzo Life Sciences, America). The Urtica Dioica group received 1.25 mg/kg of Urtica Dioica aqueous extract and the metformin group received 100 mg/kg of its solution for four weeks via gavage. Forty-eight hours after the last training session, blood samples were collected from the hearts of rats to measure glucose, Irisin, and FGF21 levels. After blood sampling, the tissues of the posterior thigh muscle, visceral fat, and white adipose tissue of each group were removed to measure PGC-1α and UCP-1 genes expression. Serum Irisin and FGF21 were measured by the ELISA method. Also, the level of PGC-1α gene expression in muscle tissue and the UCP- 1 gene expression in white and brown adipose tissue were quantitatively measured by real-time PCR method.

One-way analysis of variance test and Bonferroni post- hock test were used to compare the variables of the groups at a significant level of P<0.05. The data was analyzed using SPSS version 20 software.

Results

After the intervention, the lowest weight values were seen in the Diabetic Control group. The average blood glucose concentration of all treatment groups was significantly lower compared with the Diabetic Control

group. However, no significant difference was observed in the mean FGF21 concentration of all groups. Also, serum Irisin in all of the other groups was significantly higher than in the Diabetic Control group. UCP-1 gene expression was significantly higher in the Healthy Control group than in the Diabetic Control group.The level of PGC-1α gene expression was significantly higher in the Healthy Control group than in the Diabetic Control group.

Conclusion

In the present study, there was a significant increase in serum Irisin concentration, and the PGC-1α and UCP-1 genes expression in diabetic groups treated with Urtica Dioica extract, metformin, swimming training, combined groups, and the Healthy Control group compared to the Diabetic Control group. Although the molecular mechanism of the effects of Urtica Dioica extract has not been clearly defined, part of its effects can be attributed to lower blood glucose, decrease in blood lipids profile, reduction of the systemic inflammation indices, and preservation and regeneration of beta cells of the islets of Langerhans. Of course, to find the molecular mechanisms by which Urtica Dioica extract could affect the serum Irisin and FGF21 levels and UCP-1 and PGC-1α gene expression in diabetic rats, more studies should be done.

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Table 1. Primer sequences of rat genes that were investigated in RT-PCR assay.

Ethical Considerations:

All animal maintenance and testing procedures were carried out under the supervision of the Ethical Committee of Research Vice- Chancellor of Bushehr University of Medical Sciences.

Funding:

The present study is extracted from a doctoral dissertation and received no funding.

Authors’ contribution:

The authors have equally contributed to the article.

Conflict of interest:

There is no conflict of interest.

Acknowledgments:

We would like to express our gratitude to the esteemed Research Assistant of Islamic Azad University, Sanandaj Branch, and Bushehr University of Medical Sciences for their help and spiritual support in the implementation of this research. We also thank the reviewers for providing structural and scientific comments.

Gene Forward (5’-3’) Reverse (5’-3’)

PGC-1α 5’-CCAAAGGATGCGCTCTCGTTCA-3’ 5’-CGGTGTCTGTAGTGGCTTGACT-3’

UCP-1 5’-TGGAATAGCGGCGTGCTTG-3’ 5’-CTCATCAGATTGGGAGTAG-3’

Β-Actin 5’-CACCATTGGCAATGAGCGGTTC-3’ 5’-AGGTCTTTGCGGATGTCCACGT-3’

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ﺮﺛا 4 ﻪﺘﻔﻫ و ﺎﻨﺷ يزاﻮﻫ ﻦﯾﺮﻤﺗ ةرﺎﺼﻋ

ﯽﺑآ نﺎﯿﺑ ﺮﺑ ﻪﻧﺰﮔ نژ

ﺎﻫ ي PGC1 α و

UCP-1 و

ﺖﻈﻠﻏ ﺎﻫ ي

و ﻦﯾﺰﯾﺮﯾآ ﯽﻣﺮﺳ FGF21

ر رد ت ﺎﻫ ي قﺎﭼ ﯽﺘﺑﺎﯾد

يﺮﺒﺠﻧر سﺎﺒﻋ ،1

ﻟﺎﺧ ﺖﻣﻼﺳ هدازﺪﻤﺤﻣ ﺪﯿ

2

، هدازﺮﺒﮐا ﺪﻤﺻ ،3

ﺎﺿﺮﻣﻼﻏ ﯽﺴﯿﻤﺧ رﻮﭘ ،4

ﯽﻤﯿﻌﻧ زوﺮﻬﺑ

5

1 هوﺮﮔ .

،ﯽﺷزرو مﻮﻠﻋ و ﯽﻧﺪﺑ ﺖﯿﺑﺮﺗ دازآ هﺎﮕﺸﻧاد

ﻣﻼﺳا

،ﯽ جﺪﻨﻨﺳ ﺪﺣاو ، جﺪﻨﻨﺳ ناﺮﯾا ، . :ﻪﻣﺎﻧﺎﯾار ranjbariabas@yahoo.com

2 . ﻮﻧ ﯾ ﺪﻨﺴ ة ﺴﻣ ﺆ لو هوﺮﮔ،

،ﯽﺷزرو مﻮﻠﻋ و ﯽﻧﺪﺑ ﺖﯿﺑﺮﺗ دازآ هﺎﮕﺸﻧاد

،ﯽﻣﻼﺳا جﺪﻨﻨﺳ ﺪﺣاو

، جﺪﻨﻨﺳ

، ناﺮﯾا . :ﻪﻣﺎﻧﺎﯾار kh.mohamadzadeh@gmail.com

3 ﺷﻮﯿﺑ هوﺮﮔ . ﺮﻬﺷﻮﺑ ﯽﮑﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ﯽﻤﯿ ناﺮﯾا ،ﺮﻬﺷﻮﺑ ،

. :ﻪﻣﺎﻧﺎﯾار s.akbarzadeh@bpumc.ac.ir

نﻮﺧ هوﺮﮔ4.

و ﯽﺳﺎﻨﺷ نﻮﺧ ﮏﻧﺎﺑ ﺪﮑﺸﻧاد ة ﺮﻬﺷﻮﺑ ﯽﮑﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ،ﯽﮑﺷﺰﭘاﺮﯿﭘ ناﺮﯾا ،ﺮﻬﺷﻮﺑ ،

. :ﻪﻣﺎﻧﺎﯾار R.khamisipour@bpumc.ac.ir

ﺮﮔ 5.

چرﺎﻗ هو ﯽﮑﺷﺰﭘ ﯽﺳﺎﻨﺷ ﺮﻬﺷﻮﺑ ﯽﮑﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد

ناﺮﯾا ،ﺮﻬﺷﻮﺑ ، :ﻪﻣﺎﻧﺎﯾار

b.naeimi@bpumc.ac.ir

ﻪﻟﺎﻘﻣ تﺎﻋﻼﻃا هﺪﯿﮑﭼ

:ﻪﻟﺎﻘﻣ عﻮﻧ ﯽﺸﻫوﮋﭘ

رﺎﺗ ﯾ ﺦ رد ﯾ ﺖﻓﺎ : 21 / 04 / 1401

:يﺮﮕﻧزﺎﺑ ﺦﯾرﺎﺗ 28

/ 11 / 1401

رﺎﺗ ﯾ ﺦ ﺬﭘ ﯾ شﺮ : 02 / 12 / 1401

رﺎﺗ ﯾ ﺦ رﺎﺸﺘﻧا : 02 / 12 / 1401

ﺪﯿﻠﮐ هژاو :ﺎﻫ ،يزاﻮﻫ ﻦﯾﺮﻤﺗ

،ﺖﺑﺎﯾد

،ﻦﯾﺰﯾﺮﯾآ PGC1α

، UCP-1

، ةرﺎﺼﻋ ﻪﻧﺰﮔ

، ﻣﺪﻘﻣ

:ﻪ و ﯽﺷزرو ﺖﯿﻟﺎﻌﻓ ةرﺎﺼﻋ

ﻪﻧﺰﮔ هﺎﯿﮔ ﺐﺟﻮﻣ نﺎﯿﺑ ﺶﯾاﺰﻓا نژ

ﺎﻫ ﺖﻓﺎﺑ ﻞﯾﺪﺒﺗ رد ﺮﯿﮔرد ي ﭼ

ﺪﯿﻔﺳ ﯽﺑﺮ ﺑﻪ ﭼ ﺖﻓﺎﺑ ﯽﺑﺮ

هﻮﻬﻗ ا ي ﻣ ﯽ دﻮﺷ

، .ﺖﺴﯿﻧ ﺺﺨﺸﻣ درﻮﻣ ﻦﯾا رد ﺎﻬﻧآ ﯽﺒﯿﮐﺮﺗ ﺮﺛا ﺎﻣا رﺮﺑ ﺮﺿﺎﺣ ﺶﻫوﮋﭘ زا فﺪﻫ

ﺮﺛا ﯽﺳ ﺎﺼﻋ ةر ﮔ هﺎﯿﮔ و ﻪﻧﺰ

ﻦﯾﺮﻤﺗ يزاﻮﻫ ﺮﺑ ،ﻦﯾﺰﯾﺮﯾآ ﯽﻣﺮﺳ ﺖﻈﻠﻏ

FGF21

نژ نﺎﯿﺑ و

PGC1α UCP1,

رد تر ﺎﻫ دﻮﺑ ﯽﺘﺑﺎﯾد ي .

:ﺶﻫوﮋﭘ شور رد

ﯽﺑﺮﺠﺗ ﯽﯾﺎﻣزآرﺎﮐ ﮏﯾ 70

نزو ﻦﯿﮕﻧﺎﯿﻣ) رﺎﺘﺴﯾو داﮋﻧ ﺮﻧ تر ﺮﺳ 52

/ 18 35± / 227 (مﺮﮔ ﯽﻓدﺎﺼﺗ ﻪﺑ

ﺖﻔﻫ هوﺮﮔ ﻞﻣﺎﺷ ﻢﻟﺎﺳ لﺮﺘﻨﮐ ،

ﮐ ﯽﺘﺑﺎﯾد لﺮﺘﻨ

، ةرﺎﺼﻋ ﻪﻧﺰﮔ ، ﻦﯿﻣرﻮﻔﺘﻣ

، ﻦﯾﺮﻤﺗ ، ﻦﯾﺮﻤﺗ+ﻪﻧﺰﮔ و

ﻦﯿﻣرﻮﻔﺘﻣ ﺗ ﻦﯾﺮﻤﺗ+

ﻢﯿﺴﻘ

.ﺪﻧﺪﺷ هوﺮﮔ ﺎﻫ ي ﻪﺑ ﯽﻨﯾﺮﻤﺗ تﺪﻣ ﻪﺘﻔﻫ رﺎﻬﭼ ، ﻪﺘﻔﻫ ﺮﻫ ﺞﻨﭘ زورﺮﻫ ،زور زا

15 رد) ﻪﻘﯿﻗد ﻫ ﮥﺘﻔ ﺎﺗ (لوا 50 ﻗد ﯿ ﻪﻘ ) رد ﺘﻔﻫ ﮥ

مرﺎﻬﭼ ( ﺮﻤﺗ ﯾ ﻦ ﺎﻨﺷ ي رﺎﺒﺟا ي مﺎﺠﻧا ﺪﻧداد . هوﺮﮔ ﺎﻫ ي ﻪﻧﺰﮔ 25 /1 ﻦﯿﻣرﻮﻔﺘﻣ و 100

ﯽﻠﯿﻣ نﺪﺑ نزو زا مﺮﮔﻮﻠﯿﮐ ﺮﻫ ﺮﺑ مﺮﮔ

ار هدﺎﻣ ود ﻦﯾا زا دﻮﺧ ﻪﻧازور

ﻪﺑ ترﻮﺻ ﻪﻠﺧاﺪﻣ نﺎﯾﺎﭘ زا ﺲﭘ .ﺪﻧدﺮﮐ ﺖﻓﺎﯾرد ژاوﺎﮔ ﻧ

ﮥﻧﻮﻤ نﻮﺧ ، ﺎﺑ ﺖﻓ ﺎﻫ ي ﮥﻠﻀﻋ ،نار ﺖﺸﭘ

تر (ﯽﯾﺎﺸﺣا)ﯽﺑﺮﭼ و ساﺮﮑﻧﺎﭘ ﺎﻫ

ﺶﻫوﮋﭘ يﺎﻫﺮﯿﻐﺘﻣ ﯽﺳرﺮﺑ ياﺮﺑ ﺪﺷ ﻪﺘﻓﺮﮔ

. ﺎﯾراو ﻞﯿﻠﺤﺗ نﻮﻣزآ ﮑﯾ ﺲﻧ

آ و ﻪﻓﺮﻄ نﻮﻣز

ﯽﺒﯿﻘﻌﺗ يرادﺎﻨﻌﻣ ﺢﻄﺳ ﺎﺑ ﯽﻧوﺮﻔﻨﺑ 05

/0 ، ﻪﺑ رﻮﻈﻨﻣ تاﺮﯿﯿﻐﺗ ﯽﺳرﺮﺑ هداد

ﺎﻫ .ﺪﺷ هدﺎﻔﺘﺳا

ﯾ ﻪﺘﻓﺎ ﺎﻫ : ﯽﺘﺑﺎﯾد لﺮﺘﻨﮐ هوﺮﮔ ﺎﺑ ﻪﺴﯾﺎﻘﻣ رد ﻦﯾﺮﻤﺗ+ﻦﯿﻣرﻮﻔﺘﻣ هوﺮﮔ ﻦﯾﺰﯾﺮﯾآ ﺖﻈﻠﻏ ﻪﺑ

رﻮﻃ يرادﺎﻨﻌﻣ ﺑ ﺮﺘﺸﯿﺑ دﻮ

) 001 /0

= (P

. توﺎﻔﺗ رادﺎﻨﻌﻣ ي ﺖﻈﻠﻏ ناﺰﯿﻣ رد ﺰﯿﻧ

FGF21

مﺮﺳ هوﺮﮔ ﺎﻫ ﺶﯾاﺰﻓا .ﺪﺸﻧ هﺪﻫﺎﺸﻣ نﺎﯿﺑ رادﺎﻨﻌﻣ

نژ ﺎﻫ ي

PGC1α

و

UCP-1

رد ﮥﻤﻫ هوﺮﮔ ﺎﻫ ي هﺪﻫﺎﺸﻣ ﯽﺘﺑﺎﯾد لﺮﺘﻨﮐ و ﻢﻟﺎﺳ لﺮﺘﻨﮐ هوﺮﮔ ﻪﺑ ﺖﺒﺴﻧ نﺎﻣرد ﺪﺷ

) 05 /0

<

.( P

ﺘﻧ ﯿ ﻪﺠ ﮔﯿ ﺮ ي : نﺎﻣﺰﻤﻫ فﺮﺼﻣ ةرﺎﺼﻋ

ﺎﻨﺷ ﻦﯾﺮﻤﺗ و ﻪﻧﺰﮔ ﺐﺟﻮﻣ

و نﻮﺧ ﺰﮐﻮﻠﮔ ﺶﻫﺎﮐ لﺎﻌﻓ ﻦﯿﻨﭽﻤﻫ

ﺷ نﺪ نﺎﯿﺑ

ژ ن ﺎﻫ ي

PGC-1α

و

UCP-1

ﻣ ﯽ دﻮﺷ ﻪﮐ ﺪﻧور رد هﻮﻬﻗ ا ي نﺪﺷ ﺖﻓﺎﺑ ﯽﺑﺮﭼ ﺪﻧراد ﺶﻘﻧ .

دﺎﻨﺘﺳا : ﺮﺒﺠﻧر ي

، سﺎﺒﻋ

؛ ﺖﻣﻼﺳ هدازﺪﻤﺤﻣ ،

ﻟﺎﺧ ﯿﺪ

؛ هدازﺮﺒﮐا

، ﺪﻤﺻ

؛ ﻤﺧ ﯿ ﺴ ﯽ رﻮﭘ

، ﺎﺿﺮﻣﻼﻏ

؛ ﻌﻧ و ﯿﻤ ﯽ

، ﺮﺛا .زوﺮﻬﺑ رﺎﻬﭼ ﺮﻤﺗ ﻪﺘﻔﻫ ﯾ ﻦ زاﻮﻫ ي ﺎﻨﺷ و ةرﺎﺼﻋ ﺑآ ﯽ ﮔ ﺑ ﺮﺑ ﻪﻧﺰ ﯿ نﺎ

ژ ن ﺎﻫ ي PGC1α و UCP-1 ﺖﻈﻠﻏ و ﺎﻫ ي ﻣﺮﺳ ﯽ آﯾ ﺮﯾ ﺰﯾ ﻦ و FGF21 تر رد ﺎﻫ ي د ﯾ ﺘﺑﺎ ﯽ .قﺎﭼ ﻠﺠﻣ ﮥ ز مﻮﻠﻋ ﯾ ﺘﺴ ﯽ ﺷزرو ﯽ . 1402

؛ 14 )4 :(

67-80 .

https://doi.org/10.22059/jsb.2023.345715.1546

DOI:

ﺎﻧ :ﺮﺷ تارﺎﺸﺘﻧا .ناﺮﻬﺗ هﺎﮕﺸﻧاد

.نﺎﮔﺪﻨﺴﯾﻮﻧ©

(5)

ﺮﺛا 4 ﻪﺘﻔﻫ هرﺎﺼﻋ و ﺎﻨﺷ يزاﻮﻫ ﻦﯾﺮﻤﺗ ﯽﺑآ

ﻪﻧﺰﮔ ﺑ ﺮﺑ ﯿ نﺎ ﺎﻬﻧژ ي PGC1α و UCP-1 ...

71

ﻪﻣﺪﻘﻣ ﺑﯿ رﺎﻤ ي د ﯾ

،ﺖﺑﺎ ﺎﺷ ﯾﻊ ﺮﺗ ﯾ ﻦ ﺑﯿ رﺎﻤ ي ﻟﻮﺑﺎﺘﻣ ﯿ ﮏ رد نﺎﻬﺟ ﻪﺑ رﺎﻤﺷ ﻣ ﯽ ﯾآ ﺪ ﻧﺎﻬﺟ ﺖﺷاﺪﻬﺑ نﺎﻣزﺎﺳ و ﯽ

ﺐﻘﻟ نآ ﻪﺑ

» ﻪﻤﻫ ﮔﯿ ﺮ ي ﻪﺘﻔﻬﻧ

« 1

هداد

ﺑ .ﺖﺳا ﯿ رﺎﻤ ي د ﯾ ﺖﺑﺎ ﺧ ﯿﻠ ﯽ ﺰﻫﺮﭘ ﯾ ﻪﻨ

،ﺖﺳا ﺑ ﻪ رﻮﻃ ي ﻪﮐ ﺮﺑ شراﺰﮔ سﺎﺳا ﯽﻧﺎﻬﺟ ﺖﺷاﺪﻬﺑ نﺎﻣزﺎﺳ

دوﺪﺣ 16 ﺑ جرﺎﺨﻣ ﺪﺻرد ﯿ

ﻧﺎﺘﺳرﺎﻤ ﯽ طﻮﺑﺮﻣ

ﺑ ﻪﺑ ﯿ رﺎﻤ ي د ﯾ ﺖﺑﺎ ﺮﺑ ﻎﻟﺎﺑ ﺮﺿﺎﺣ لﺎﺣ رد و ﺖﺳا 12

ﺪﺻرد ﺟدﻮﺑ ﮥ ﺑ فﺮﺻ نﺎﻬﺟ ﺖﺷاﺪﻬﺑ و ﺖﻣﻼﺳ ﯿ

رﺎﻤ ي د ﯾ ﺖﺑﺎ ﻣ ﯽ دﻮﺷ ) 1 .(

ﯾ ﮏ ﮏﺒﺳ

ﮔﺪﻧز ﯽ ﻏ ﯿ لﺎﻌﻓﺮ و ﻦﺘﺷاﺪﻧ ﻟﺎﻌﻓ ﯿ ﺖ ﻧﺪﺑ ﯽ زا ﻢﻬﻣ ﺮﺗ ﯾ ﻦ ﻗﺎﭼ زوﺮﺑ ﻞﻣاﻮﻋ ﯽ

ﺑﺮﭼ ﻊﻤﺠﺗ و ﯽ

ﺎﻫ و ﯽﭘ رد ﺑ زوﺮﺑ نآ ﯿ رﺎﻤ ي ﺎﻫ ي ﻟﻮﺑﺎﺘﻣ ﯿ ﮑ ﯽ ﺪﻨﻧﺎﻣ

دﯾ ﺖﺑﺎ ﺖﺳا ) 2 .(

ﺖﻓﺎﺑ ﺑﺮﭼ ﯽ ﻪﺑ ناﻮﻨﻋ ﺮﮐوﺪﻧا ماﺪﻧا ﯾﻨ

ﯽ لﺮﺘﻨﮐ رد لﺎﻌﻓ ﺖﺧﻮﺳ

زﺎﺳو .ﺖﺳا هﺪﺷ ﻪﺘﺧﺎﻨﺷ نﺪﺑ اﯾ

ﻦ نﺎﮔرا نورد رﯾ ﺰ ﺌﺗوﺮﭘ ﯿ ﻦ ﺎﻫ ي دﺪﻌﺘﻣ ي ار

دآ ناﻮﻨﻋ ﺖﺤﺗ ﯾ

ﺎﮐﻮﭙ ﯾ ﻦ ﻟﻮﺗ ﯿﺪ ﺢﺷﺮﺗ و ﻣ ﯽ ﺪﻨﮐ رد ﻪﮐ ژﺮﻧا زﺎﺘﺳﻮﻤﻫ ي

و ﺖﺧﻮﺳ زﺎﺳو ﻫﻮﺑﺮﮐ ﯿ تارﺪ ﺮﭼ و ﺑ ﯽ ﻤﻬﻣ ﺶﻘﻧ ﯽ

اﯾ ﻔﺎ ﻣ ﯽ ﺪﻨﻨﮐ ) 3 .(

ﺑﺮﭼ ﯽ ود ﻪﺑ نﺪﺑ رد رﻮﺻ

ت ﺑﺮﭼ ﯽ ﻔﺳ ﯿﺪ و هﻮﻬﻗ ا ي ﺑﺮﭼ ﻪﮐ دراد دﻮﺟو ﯽ

هﻮﻬﻗ ا ي ﻞﻤﻋ فﻼﺧ ﺮﺑ ﺧذ

ﯿ هﺮ زﺎﺳ ي ﻪﮐ وﯾ ﮋة ﺑﺮﭼ ﯽ ﻔﺳ ﯿﺪ

،ﺖﺳا

ﻪﺑ ﺑ ﺖﻠﻋ ﯿ نﺎ ﺌﺗوﺮﭘ ﯿ ﻦ ﺖﻔﺟ هﺪﺸﻧ - 1

(UCP-1)

اﺰﻓا و ﯾ ﺶ ﻣ ﻢﮐاﺮﺗ ﯿ رﺪﻨﮐﻮﺘ ﯾﺎ ﯽﯾ

، ازﺎﻣﺮﮔ ﺶﻘﻧ ﯽﯾ

اد رد و ﺐﺟﻮﻣ ﺪﺒﺗ ﯾ ﻞ ژﺮﻧا ي ﺷ ﯿﻤ ﯿﺎﯾ ﯽ ﻪﺑ

ﺗراﺮﺣ ﯽ ﻣ ﯽ دﻮﺷ ) 4 .(

ﯾ ﻪﺘﻓﺎ ﺎﻫ نﺎﺸﻧ ﻣ ﯽ ﺪﻫد ﻪﮐ ﺑﺮﭼ ﺖﻓﺎﺑ راﺪﻘﻣ ﯽ

هﻮﻬﻗ ا ي قﺎﭼ داﺮﻓا رد ﻪﺑ

رﻮﻃ رادﺎﻨﻌﻣ ي ﺮﺘﻤﮐ ﺖﺳا ﺑ و ﯿ ﻦ ﺑﺮﭼ ﺖﻓﺎﺑ ﯽ هﻮﻬﻗ ا ي ﺺﺧﺎﺷ و

دﻮﺗ ة ﻧﺪﺑ ﯽ ﺑﺮﭼ ﺪﺻرد و ﯽ

ﻔﻨﻣ طﺎﺒﺗرا ﯽ

دراد دﻮﺟو )

5 رﺎﮐوزﺎﺳ سﺎﺳا ﺮﺑ .(

ﻟﻮﮑﻟﻮﻣ ﯽ ﺪﺟ ﯾﺪ نآ رد ﻪﮐ

PGC-1α

ﺪﺒﺗ ،دراد ﺶﻘﻧ ﯾ

ﻞ ﺑﺮﭼ ﺖﻓﺎﺑ ﯽ

ﻔﺳ ﯿﺪ ﻪﺑ هﻮﻬﻗ ا ي ﺘﻧ رد و ﯿ ﻪﺠ اﺰﻓا ﯾ ﺶ ازﺎﻣﺮﮔ ﯽﯾ ﺖﺳا هﺪﺷ هﺪﻫﺎﺸﻣ نزو ﺶﻫﺎﮐ و .

PGC-1α

لﺎﻌﻓ ﺪﻨﻨﮐ ة ﻮﻧور ﻞﻣﺎﻋ ﯾ

ﺴ ﯽ

PPAR

ﻪﮐ ﺖﺳا

ﺴﺑ ﯿ رﺎ ي زا تاﺮﯿﺛﺄﺗ ﺑﯿ ژﻮﻟﻮ ﯾﮑ ﯽ دﻮﺧ ار ﺮﺑ ﺖﺧﻮﺳ زﺎﺳو ژﺮﻧا ي ا زا ﯾ ﻦ ﺮﻃ ﯾ ﻖ لﺎﻤﻋا ﻣ ﯽ ﺪﻨﮐ ) 6 ﺶﻫوﮋﭘ .(

ﺎﻫ نﺎﺸﻧ هداد اﺰﻓا ﻪﮐ ﺖﺳا ﯾ

ﺶ ﺑﯿ نﺎ

نژ

α 1 -

ﺐﺟﻮﻣPGC

اﺰﻓا ﯾ ﺶ ﺑﯿ نﺎ ﻞﻣﺎﻋ ﺪﻧرﺬﮔ ة ﻏ ﺎﺸ ﯽﯾ

52

ﯽﻣFNDC

دﻮﺷ ﻪﮐ اﯾ ﻦ ﺌﺗوﺮﭘ ﯿ ﻦ ﺎﺸﻏ زا ،ﻦﺘﺴﮑﺷ زا ﺲﭘ ي

ﻟﻮﻠﺳ ﯽ جرﺎﺧ

ﺤﻣ رد و هﺪﺷ ﯿ

ﻂ جرﺎﺧ ﻟﻮﻠﺳ ﯽ دازآ ﯽﻣ دﻮﺷ آ و ﯾﺮ ﯾﺰ ﯾ ﻦ نآ زا لﻮﻠﺤﻣ ﮑﺸﺗ

ﯿ ﻞ ﻣ ﯽ ﺪﻫد آ . ﯾﺮ ﯾﺰ ﯾ ﻦ ﺖﻓﺎﺑ زا ﺎﻫ ﯾ ﯽ ﻠﮐ ،ﺪﺒﮐ ﺪﻨﻧﺎﻣ ﯿ

،ﻪ ﻠﻀﻋ ﮥ و ﺐﻠﻗ

ﺢﺷﺮﺗ ﺖﺳﻮﭘ ﻣ

ﯽ ﻮﺷ د آ نﻮﻣرﻮﻫ . ﯾﺮ

ﯾﺰ ﯾ ﻦ ﺐﺟﻮﻣ ﺑﯿ نﺎ نژ

UCP-1

ﺑﺮﭼ ﺖﻓﺎﺑ رد ﯽ

هﻮﻬﻗ ا ي ﻣ ﯽ دﻮﺷ آ . ﯾﺮ ﯾﺰ ﯾ ﻦ د ﺎﺑ ﻊﻗاو ر ﺛﺄﺗ ﯿﺮ ﺑﺮﭼ ﺖﻓﺎﺑ ﺮﺑ ﯽ

ﻔﺳ ﯿﺪ و هﻮﻬﻗ ا ي

، ژﺮﻧا ي ﻓﺮﺼﻣ ﯽ اﺰﻓا ار ﯾ ﺶ ﻣ ﯽ ﺪﻫد نزو ﺶﻫﺎﮐ ﻪﺑ و ﺮﺠﻨﻣ

ﻣ ﯽ دﻮﺷ ) 8،7،5 ﻤﻫ رد .(

ﯿ ﻦ ﻣز ﯿ ﻪﻨ ﺞﯾﺎﺘﻧ رد ﻪﻌﻟﺎﻄﻣ ا ي ﺧا ﻪﮐ ﯿ

ًاﺮ

ور ي د داﺮﻓا ﯾ ﺘﺑﺎ ﯽ مﺎﺠﻧا ﺘﻓﺮﮔ ﻪ ﺖﻈﻠﻏ ، آ ﻢﮐ ﯾﺮ ﯾﺰ ﯾ ﻦ ا رد ﯾ ﻦ ﺑﯿ نارﺎﻤ اﺮﻓا ﻪﺑ ﺖﺒﺴﻧ د

دﻮﺑ هﺪﺷ شراﺰﮔ ﻢﻟﺎﺳ )

9 .(

هوﻼﻋ آ ﺮﺑ ﯾﺮ ﯾﺰ ﯾ ﻦ ﻪﺑ ﮔزﺎﺗ ﯽ ﻓ ﺪﺷر رﻮﺘﮐﺎﻓ ﯿ

ﺖﺳﻼﺑوﺮﺒ 21

)

FGF-21

( ﻧﯿ ﺰ ﻪﺑ ناﻮﻨﻋ ﻞﻣﺎﻋ ﺮﺛﺆﻣ ﺖﺳا هﺪﺷ ﻪﺘﺧﺎﻨﺷ ﺰﮐﻮﻠﮔ زﺎﺘﺳﻮﻤﻫ رد .

FGF-21

ﯾ ﮏ زا ﻮﻀﻋ داﻮﻧﺎﺧ ة ﺎﻫرﻮﺘﮐﺎﻓ ي ﻓ ﺪﺷر ﯿ ﺘﺳﻼﺑوﺮﺒ ﯽ ﻪﮐ ﺖﺳا ﺐﻠﻏا ﺑﺮﭼ ﺖﻓﺎﺑ رد ﯽ

ﻨﭽﻤﻫ و ﯿ ﻦ ﺑ ساﺮﮑﻧﺎﭘ و ﺪﺒﮐ ﯿ

نﺎ ﻣ ﯽ دﻮﺷ و

اﺰﻓا ﺖﺳا هﺪﺷ هداد نﺎﺸﻧ ﯾ

ﺶ دﺎﻘﻣ ﯾﺮ ﺧ ﻧﻮ ﯽ ﻤﻬﻣ ﺶﻘﻧ نآ ﯽ

ﻈﻨﺗ رد ﯿ ﻢ ﺖﺧﻮﺳ زﺎﺳو ﺑﺮﭼ و ﺰﮐﻮﻠﮔ ﯽ

دراد ﺶﻘﻧ )

10 ﺶﻫوﮋﭘ .(

ﺎﻫ رد لﺪﻣ ﺎﻫ ي

ﺣﯿ ﻧاﻮ ﯽ نﺎﺸﻧ هداد ﺪﻧا اﺰﻓا ﻪﮐ ﯾ ﺶ ﺑﯿ نﺎ اﯾ ﻦ رﻮﺘﮐﺎﻓ ﻪﺑ ترﻮﺻ راﺮﺗ ﯾ ﻪﺘﺨ 3 ﺮﻃ زا ﯾ ﻖ اﺰﻓا ﯾ ﺶ ﺰﻫ ﯾﻨ ﮥ ژﺮﻧا ي ﺐﺟﻮﻣ ﺑﺮﭼ ﺶﻫﺎﮐ ﯽ

ﻣ ﯽ دﻮﺷ ) 11 .(

ﺳرﺮﺑ ﯽ ﺎﻫ ي ﺪﺟ ﯾﺪ نﺎﺸﻧ ﻣ ﯽ ﺪﻫد ﺑﺮﭼ ﺖﻓﺎﺑ ود ﺮﻫ ﻪﮐ ﯽ

ﻔﺳ ﯿﺪ و هﻮﻬﻗ ا ي ﺮﺤﺗ ﻪﺑ ﯾ ﮏ

FGF-21

سﺎﺴﺣ ا ﺪﻧ ) 12 ﺎﺑ نﺎﻣرد .(

FGF-21

ﺐﺟﻮﻣ

اﺰﻓا ﯾ ﺶ ﺑﯿ نﺎ

UCP-1

ﯽﻣ دﻮﺷ دآ رﻮﻬﻇ و ﯾ

ﺳﻮﭙ ﯿ ﺖ ﺎﻫ ي هﻮﻬﻗ ا ي ﺑﺮﭼ رد ار ﯽ ز ﯾ ﺪﻠﺟﺮ ي اﺰﻓا ﯾ ﺶ ﻣ ﯽ ﺪﻫد ) 13 ﻟﺎﻌﻓ .(

ﯿ ﺖ ﻧﺪﺑ ﯽ

ﺐﺟﻮﻣ ﺑﺮﭼ ﺶﻫﺎﮐ ﯽ

ﻔﺳ ﯿﺪ ﺪﺒﺗ و ﯾ ﻞ ﺑﺮﭼ ﻪﺑ نآ ﯽ

هﻮﻬﻗ ا ي ﺮﻃ زا ﯾ ﻖ اﺰﻓا ﯾ ﺶ ﺑﯿ نﺎ نژ ﺎﻫ ي

PGC1α ,UCP-1

آ و ﯾﺮ ﯾﺰ ﯾ ﻦ ﻣ ﯽ دﻮﺷ ) 14 .(

ﻪﺑ ﺮﻈﻧ ﻣ ﯽ ﺪﺳر ﺸﺨﺑ ﯽ ﺮﻤﺗ ﺮﺛا رﺎﮐوزﺎﺳ زا ﯾ

ﻦ ﺷزرو ﯽ اﺰﻓا ﻪﺑ ﯾ ﺶ ﺑﯿ نﺎ ﻞﻣاﻮﻋ ﺰﺘﻨﺳ و هرﺎﺷا

هﺪﺷ ﺪﺷﺎﺑ ﻂﺒﺗﺮﻣ ا رد .

ﯾ ﻦ ﻣز ﯿ ﻪﻨ نﺎﺸﻧ هﺪﺷ هداد

ﺖﺳا رﺎﻬﭼ ﻪﺘﻔﻫ ﺮﻤﺗ ﯾ ﻦ ﺘﻣﺎﻘﺘﺳا ﯽ ﺎﻨﺷ ﻣ ﯽ ﺪﻧاﻮﺗ ﺐﺟﻮﻣ اﺰﻓا ﯾ ﺶ رادﺎﻨﻌﻣ ي رد ﺑﯿ نﺎ

PGC-1α

ﺖﻓﺎﺑ ﺑﺮﭼ ﯽ ﻔﺳ ﯿﺪ دﻮﺷ ) 15 (.

ﺑ ا دﻮﺟو ﺎ ﯾ

،ﻦ 12

ﻪﺘﻔﻫ ﺮﻤﺗ ﯾ ﻦ ﺘﻣﺎﻘﺘﺳا ﯽ رد نادﺮﻣ اراد ي ﻪﻧﺎﺸﻧ ﺎﻫ ي ﭘﯿ ﺶ دﯾ ﺘﺑﺎ ﯽ

، ﻐﺗ ﯿﯿ ﺮ رادﺎﻨﻌﻣ ي رد ﻣﯿ ناﺰ ﺑﯿ نﺎ

PGC-1α

اﯾ دﺎﺠ ) دﺮﮑﻧ 16 .(

هوﻼﻋ ا ﺮﺑ ﯾ

،ﻦ

FGF21

ﺖﺤﺗ ﺛﺄﺗ ﯿﺮ ﺮﻤﺗ ﯾ ﻦ ﺷزرو ﯽ راﺮﻗ ﻣ ﯽ ﮔﯿ دﺮ ﺑ رد و ﯿ رﺎﻤ ي ﺎﻫ ي ﻟﻮﺑﺎﺘﻣ ﯿ ﻤﺴ ﯽ ﻈﻧ ﯿﺮ د ﯾ ﺖﺑﺎ عﻮﻧ ود ﻣ ﯽ ﺪﻧاﻮﺗ ﺛﺄﺗ ﯿﺮ اد ﺖﺒﺜﻣ ﻪﺘﺷ ﺪﺷﺎﺑ .

اﺮﺑﺎﻨﺑ ﯾ ﻦ رد ﻣز ﯿ ﮥﻨ تاﺮﯿﺛﺄﺗ ﺮﻤﺗ ﯾ ﻦ ﺷزرو ﯽ ﻟﻮﮑﻟﻮﻣ ﻞﻣاﻮﻋ ﺮﺑ ﯽ

ﺧد ﻢﻬﻣ ﯿ ﻞ رد هﻮﻬﻗ ا ي ﺑﺮﭼ ﺖﻓﺎﺑ نﺪﺷ ﯽ

ﻔﺳ ﯿﺪ ﺶﻫوﮋﭘ ﺎﻫ ي ﺑﯿ ﺮﺘﺸ ي ﺎﺑ ﯾﺪ

ﮔ مﺎﺠﻧا ﯿ دﺮ

.

1. Latent Epidemiology

2. Transmembrane

3. Transgenic

(6)

72 نﺎﺘﺴﻣز ،مرﺎﻬﭼ ةرﺎﻤﺷ ،ﻢﻫدرﺎﻬﭼ ةرود ،ﯽﺷزرو ﯽﺘﺴﯾز مﻮﻠﻋ

1401

ﺪﻨﭼﺮﻫ رد لﺎﺣ ﺮﺿﺎﺣ ﻠﺻا نﺎﻣرد ﯽ

اﺮﺑ ﺮﺛﺆﻣ و ي

د نﺎﻣرد ﯾ

،ﺖﺑﺎ ﻟﻮﺴﻧا زا هدﺎﻔﺘﺳا ﯿ

ﻦ ﻫ ﻞﻣاﻮﻋ و ﯿ

ﻠﮔﻮﭙ ﯿ ﺴ ﯿﻤ ﯿ ﮏ ﻠﮔ ﺪﻨﻧﺎﻣ ﯽ - ﻦﺑ ﻣﻼﮐ ﯿ

،ﺪ

ﺗﯿ ﻟوزﺎ ﯿﺪ ﯾ ﻦ ﯾد نﻮ

،ﺎﻫ ﻣرﻮﻔﺘﻣ ﯿ ﻦ ﻧﻮﻔﻟﻮﺳو ﯿ ﻞ هروا ﺎﻫ ﻟو ،ﺖﺳ ﯽ اﯾ ﻦ ﮐﺮﺗ ﯿ تﺎﺒ اراد ي دﺪﻌﺘﻣ بﻮﻠﻄﻣﺎﻧ ضراﻮﻋ ي

ﺪﻨﺘﺴﻫ ﻞﻣاﻮﻋ ﺮﺑ تﺪﻣزارد رد و

ناﻮﺗﺎﻧ ﺪﻨﻨﮐ ة ﺷﺎﻧ ﯽ د زا ﯾ

،ﺖﺑﺎ ﻗوﺮﻋ تﻼﮑﺸﻣ ﻞﺜﻣ ﯽ

زوﺮﻠﮑﺳاوﺮﮐﺎﻣ و

ﻣ ﯿ زوﺮﻠﮑﺳاوﺮﮑ ﺛﺄﺗ

ﯿﺮ ي ﺷور و ﺪﻧراﺪﻧ ﯽ

ﺰﻫﺮﭘ ﯾ ﻪﻨ ﻪﺘﺴﺧ و رﺎﺛآ ﺎﺑ هﺪﻨﻨﮐ

ﺒﻧﺎﺟ ﯽ ﻧاور و ﯽ ﻫ ناواﺮﻓ هاﺮﻤ ﺪﻧا ) 17 زا هدﺎﻔﺘﺳا .(

شور ﺎﻫ ي ﻧﺎﻣرد ﯽ ﻢﮐ و ﻢﻟﺎﺳ ﻪﺿرﺎﻋ

ﻨﭽﻤﻫ و ﺮﺗ ﯿ

ﻦ ﺘﻨﺳ ﺐﻃ زا هدﺎﻔﺘﺳا ﯽ

ﮔ و ﯿ نﺎﻫﺎ وراد ﯽﯾ

ياﺮﺑ نﺎﻣرد ﺑﯿ رﺎﻤ ي ﺎﻫ ي ﻮﮔ نﻮﮔﺎﻧ نﺎﻃﺮﺳ نﻮﭽﻤﻫ ،ﺎﻫ

ﺑﯿ رﺎﻤ ي ﺎﻫ ي ﺒﻠﻗ ﯽ - ﻗوﺮﻋ ﯽ ﺘﺣ و ﯽ ﺬﻐﺗ ﯾﮥ ﮐارﻮﺧ ﯽ اﺰﻓا ﺮﺿﺎﺣ لﺎﺣ رد ﯾ

ﺶ ﭘﯿ اﺪ هدﺮﮐ و

ز ناراﺪﻓﺮﻃ ﯾ

دﺎ ي ﻪﺑ نﺎﻬﺟ مﺎﻤﺗ رد ار بﺬﺟ دﻮﺧ

ﺖﺳا هدﺮﮐ ا رد .

ﯾ ﻦ ﻣ ﯿ نﺎ ﮔ ﯿ هﺎ ﻤﻠﻋ مﺎﻧ ﺎﺑ ﻪﻧﺰﮔ ﯽ

Urticadioica

ﺘﻨﺳ ﺐﻃ رد ﯽ

اﯾ ناﺮ و

ﺎﻫرﻮﺸﮐ ي د ﯾ ﺮﮕ هدﺎﻔﺘﺳا ﻣ ﯽ دﻮﺷ و ﻪﺑ ناﻮﻨﻋ ﮔ ﯿ هﺎ ﺪﻗ نﻮﺘﻣ رد نﻮﺧ ﺪﻨﻗ ﺶﻫﺎﮐ رد ﺮﺛﺆﻣ ﯾﻤ

ﯽ ﮑﺷﺰﭘ ﯽ ﺖﺳا هﺪﺷ هدروآ )

18 .(

ﻘﯿﻘﺤﺗ تﺎ ﺣﯿ ﻧاﻮ ﯽ نﺎﺸﻧ هداد ﺪﻧا ﻪﮐ ﮐﺮﺗ ﯿ تﺎﺒ ﻪﻧﺰﮔ ﺪﻨﻧﺎﻣ ﺋﻮﻧووﻼﻓ ﯿ ﺎﻫﺪ و ﻟﺎﺳ ﯿ ﺴ ﯿﻠﯿ ﮏ ﺳا ﯿﺪ ﻣ ﯽ ﺪﻧاﻮﺗ ﺢﻄﺳ ﻟﻮﺴﻧا ﯿ ﻦ رد ار نﻮﺧ دﯾ

ﺖﺑﺎ

ﺒﻃ ﯿﻌ ﯽ و دﯾ ﺖﺑﺎ اﯾ هﺪﺷدﺎﺠ ﻂﺳﻮﺗ

ﺳﻮﺗوزﻮﺘﭘﺮﺘﺳا ﯿ

ﻦ

(STZ)

دﺮﺒﺑ ﻻﺎﺑ ) 19 ﻪﻧﺰﮔ .(

ﺐﺟﻮﻣ اﺰﻓا ﯾ ﺶ ﻟﻮﺴﻧا ﺢﺷﺮﺗ ﯿ

ﻦ لﻮﻠﺳ زا ﺎﻫ ي ﺎﺘﺑ ي

ﺲﻧﺎﻫﺮﮕﻧﻻ ﯽﻣ

دﻮﺷ ﭽﻤﻫ و نﻮ ﮐﺮﺤﻣ ﯽ ﻮﻗ ي اﺮﺑ ي ﻟﻮﺴﻧا نﺪﺷ دازآ ﯿ

ﻦ لﻮﻠﺳ زا ﺎﻫ ي ﻣ ﻞﻤﻋ ﺎﺘﺑ ﯽ

ﺪﻨﮐ . ﻨﭽﻤﻫ ﯿ ﻦ ا ﺘﻈﻓﺎﺤﻣ ﺮﺛ ﯽ

ﺮﺑ ﻪﻧﺰﮔ

لﻮﻠﺳ ﺎﻫ ي ﺎﺘﺑ ي تر رد ساﺮﮑﻧﺎﭘ ﺎﻫ

ي اراد ي نﻮﺧ ﺰﮐﻮﻠﮔ ﺖﺑﺎﺛ ﻻﺎﺑ

ﺖﺳا هﺪﺷ )

20 ﻨﭽﻤﻫ .(

ﯿ ﻦ ﺎﺑ ﺛﺄﺗ ﯿﺮ ﺰﮐﻮﻠﮔ زﺎﺘﺳﻮﻤﻫ ﺮﺑ ﺐﺟﻮﻣ

بﺬﺟ رﺎﻬﻣ

هدور ا ي ﺰﮐﻮﻠﮔ ) 21 ﻟﺎﻌﻓ رﺎﻬﻣ و ،(

ﯿ ﺖ ﺰﻧآ ﯾ ﻢ ﻣآ ﺎﻔﻟآ ﯿ زﻼ ﻣ ﯽ دﻮﺷ ) 22 .(

ﻢﻬﻣ ﺮﺗ ﯾ ﻦ دﺎﻣ ة ﺮﺛﺆﻣ ة ةرﺎﺼﻋ ﮔ ﯿ هﺎ ﻟﺰﮔ ،ﻪﻧﺰﮔ ﯿ ﻦ ﺖﺳا

؛ ﻟﺰﮔ ﯿ ﻦ ﯾ ﮏ ﻘﻠﺣ ﮥ

ﺘﭙﭘ ﯿﺪ ي ﺒﺴﭼ ﺎﺑ ﻪﮐ ﺖﺳا ﯿ

نﺪ 10 ﻪﺑ نآ لﻮﮑﻟﻮﻣ ﯾ

ﺪﮑ ﯾ ﺮﮕ ،نﺪﺑ رد ﺬﻓﺎﻨﻣ ي ور ار ي ﺎﺸﻏ ي ا لﻮﻠﺳ ﯾ دﺎﺠ ﻣ ﯽ ﺪﻨﮐ و ﺐﺟﻮﻣ ﻬﺴﺗ ﯿ ﻞ ﺰﮐﻮﻠﮔ دورو

لﻮﻠﺳ ﻞﺧاد ﻪﺑ ﻣ

ﯽ دﻮﺷ ا ﻪﺑ و ﯾ ﻦ ﺗﺮﺗ ﯿ ﺐ ﺶﻫﺎﮐ نﻮﺧ ﺰﮐﻮﻠﮔ ﺖﻈﻠﻏ ﻣ

ﯽ ﯾ ﺪﺑﺎ ) 23 .(

ﻘﯿﻘﺤﺗ تﺎ ز ﯾ دﺎ ي ﯽﺳرﺮﺑ ﻪﺑ ﺮﯿﺛﺄﺗ

ﻫ ﯿ ﻠﮔﻮﭙ ﯿ ﺴ ﯿﻤ ﯿ ﮏ ﮔ ﯿ هﺎ ﻨﭽﻤﻫ و ﻪﻧﺰﮔ ﯿ

ﻦ ﺗ ﺛﺄﯿ ﺮ ﻟﺎﻌﻓ ﯿ ﺖ ﻧﺪﺑ ﯽ د ﺮﺑ ﯾ ﺖﺑﺎ ﻪﺘﺧادﺮﭘ ﺪﻧا

، ﻟو ﯽ ﺎﺗ لﺎﺣ ﻪﺑ ﺛﺄﺗ ﯿﺮ

ﻟﺎﻌﻓ نﺎﻣﺰﻤﻫ ﯿ

ﺖ ﻧﺪﺑ ﯽ فﺮﺼﻣ و ةرﺎﺼﻋ

ﺑ ﺮﺑ ﻪﻧﺰﮔ ﯿ نﺎ نژ ﺎﻫ و ﺖﻈﻠﻏ ﯿﺌﺗوﺮﭘ ﻦ ﺎﻫ ي ﺮﺛﺆﻣ ﺪﺒﺗ رد ﯾ ﻞ ﺑﺮﭼ ﺖﻓﺎﺑ ﯽ ﻔﺳ ﯿﺪ ﻪﺑ هﻮﻬﻗ ا ي ﻼﺘﺒﻣ رد ﯾ نﺎ

د ﻪﺑ ﯾ ﺖﺑﺎ ﺳرﺮﺑ درﻮﻣ ﯽ

،ﻪﺘﻓﺮﮕﻧ راﺮﻗ ﻦﯾازا

ور اﯾ ﻦ ﻖﯿﻘﺤﺗ ﻪﺑ رﻮﻈﻨﻣ ﺳرﺮﺑ ﯽ ﺮﯿﺛﺄﺗ تا ةرﺎﺼﻋ ﮔ ﯿ هﺎ ﺮﻤﺗ و ﻪﻧﺰﮔ ﯾ

ﻦ زاﻮﻫ ي ﺮﺑ ﺎﻨﺷ ﺎﻫﺮﮕﻧﺎﺸﻧ ي

ﺪﺒﺗ ﯾ ﻞ ﺑﺮﭼ ﺖﻓﺎﺑ ﯽ ﻔﺳ ﯿﺪ ﻪﺑ هﻮﻬﻗ ا ي آ) ﯾﺮ ﯾﺰ ﯾ

،ﻦ

PGC-1α,UCP-1,FGF-21

و ،(

ﺎﻘﻣ ﯾ ﺴ ﮥ ﻧآ ﺎﻬ ﺎﺑ يوراد ﺪﻨﻫﺎﮐ ة ﻣرﻮﻔﺘﻣ) نﻮﺧ ﺰﮐﻮﻠﮔ ﯿ

ﻦ ﺮﺑ (

ور ي ر ت ﺎﻫ ي د ﯾ ﺘﺑﺎ ﯽ .ﺖﻓﺮﮔ مﺎﺠﻧا

شور ﺳﺎﻨﺷ ﯽ ﺶﻫوﮋﭘ

ﻖﯿﻘﺤﺗ شور ﻪﺑ ﺮﺿﺎﺣ ﺑﺮﺠﺗ

ﯽ ور ي 70 تر ﺮﺳ اﺮﺤﺻ ﯾ ﯽ داﮋﻧ ﺮﻧ وﯾ رﺎﺘﺴ ﻣ ﺎﺑ ﯿ ﮕﻧﺎ ﯿ ﻦ نزو 52 /

±18 35 / 227 مﺮﮔ و ﮥﻨﻣاد ﻨﺳ ﯽ 2 - 3 مﺎﺠﻧا هﺎﻣ

تر .ﺖﻓﺮﮔ ﺎﻫ

ﺑ ﻪ ترﻮﺻ ﻓدﺎﺼﺗ ﯽ ﻪﺑ هوﺮﮔ ﺖﻔﻫ 10

ﺮﺳ ي وﺎﺴﻣ ي د لﺮـﺘﻨﮐ ،ﻢﻟﺎـﺳ لﺮﺘﻨﮐ ﻞﻣﺎﺷ ـﯾ

ﺘﺑﺎ ﯽ

، ةرﺎـﺼﻋ ﻣرﻮـﻔﺘﻣ ،ﻪـﻧﺰﮔ ﯿ

،ﻦ ﺮـﻤﺗ ﯾ

،ﻦ

ﺮﻤﺗ+ﻪﻧﺰﮔ ﯾ ﻦ ﻣرﻮﻔﺘﻣ و ﯿ ﻦ ﺮﻤﺗ+

ﯾ ﻦ ﺴﻘﺗ ﯿ ﻢ ﺎﺷ .ﺪﻧﺪﺷ ﯾ نﺎ اﺪﺘﺑا رد ﺖﺳا ﺮﮐذ ي

ﻫوﮋﭘ ﺶ 84 ﺎﻣا ،ﺪﺷ بﺎﺨﺘﻧا تر ﺮﺳ زا ﺲﭘ

رﺰﺗ ﯾ ﻖ

STZ 8

ﺮـﺳ

تر زا ﺎﻫ د ﯾ ﺘﺑﺎ ﯽ ﻃ و ﺪﻧﺪﺸﻧ ﯽ

ﺮﻤﺗ ﻞﮑﺗوﺮﭘ ﯾ

ﻦ ﻧﯿ ﺰ ﺶﺷ ﺎﭘ رد .ﺪﻧﺪﺷ ﻒﻠﺗ تر ﺮﺳ ﯾ

نﺎ ﻠﺤﺗ ﯿ ﻞ رﺎﻣآ ي ﺎﺑ 10 مﺎﺠﻧا هوﺮﮔ ﺮﻫ رد تر ﺮﺳ ﺖـﻓﺮﮔ

.

ﻢﻟﺎﺳ لﺮﺘﻨﮐ هوﺮﮔ ﻪﺑ

رﻮﻈﻨﻣ ﺎﻘﻣ ﯾ ﺴ ﮥ ﻐﺗ ﯿﯿ تاﺮ اﯾ هﺪﺷدﺎﺠ د لﺮﺘﻨﮐ هوﺮﮔ رد ﯾ

ﺖﺑﺎ .ﺪﺷ هﺪﻧﺎﺠﻨﮔ ﻪﻌﻟﺎﻄﻣ رد ر مﺎﻤﺗ

ت ﺎﻫ رد اﺮﺷ ﯾ ﻂ ًﻼﻣﺎﮐ ﯾ نﺎﺴﮑ و

رد ﻧﺎﮑﻣ ﯽ ﺎﺑ ﻮﻬﺗ ﯾﮥ ﺎﻣد و ،بﻮﻠﻄﻣ ي

قﺎﺗا 22 - 28 ﺟرد ﮥ ﺘﻧﺎﺳ ﯽ داﺮﮔ و 12 ﺖﻋﺎﺳ ﺎﻨﺷور ﯽﯾ و 12 ﺖﻋﺎﺳ رﺎﺗ ﯾﮑ ﯽ رد ، ﻪﺴﻔﻗ ا ي راﺪﻬﮕﻧ ﻪﻧﺎﮔاﺪﺟ ي

.ﺪﻧﺪﺷ ﻪﺑ ﺰﺟ ﻧ ﻊﻗاﻮﻣ رد ﯿ

زﺎ تر ،ﺎﺘﺷﺎﻧ نﻮﺧ ﻪﺑ ﺎﻫ

بآ ﻪﺑ و اﺬﻏ ﺳﺮﺘﺳد ﯽ اﺬﻏ .ﺪﻨﺘﺷاد ﻪﻧادازآ ي

ر ت ﺎﻫ ﻞﻣﺎﺷ ﺖﻠﭘ ﮐﺮﺗ ﺎﺑ دراﺪﻧﺎﺘﺳا ﯿ

ﺐ 10 ﺪﺻرد

ﮐﯿ ﺮﻟﺎﮐﻮﻠ ي ﺑﺮﭼ زا ﯽ

، 20 ﺪﺻرد ﮐ ﯿ ﺮﻟﺎﮐﻮﻠ ي ﺌﺗوﺮﭘ زا ﯿ

،ﻦ 70 ﺪﺻرد ﮐ ﯿ ﺮﻟﺎﮐﻮﻠ ي ﻫﻮﺑﺮﮐ ﯿ

،تارﺪ ا ،جﺮﮐ) روﺮﭙﻬﺑ ﺖﮐﺮﺷ زا ﯾ

ناﺮ ﻞـﺣاﺮﻣ مﺎﻤﺗ .دﻮﺑ (

راﺪﻬﮕﻧ ي ﺎﻣزآ و ﯾ ﺶ ﺣ ﯿ تﺎﻧاﻮ ترﺎﻈﻧ ﺖﺤﺗ ﻤﮐ

ﯿﺘ ﮥ ﻗﻼﺧا ﯽ ﺸﻫوﮋﭘ ﺖﻧوﺎﻌﻣ ﯽ

ﮕﺸﻧاد هﺎ ﮑﺷﺰﭘ مﻮﻠﻋ ﯽ

ﺖﻓﺮﮔ ترﻮﺻ ﺮﻬﺷﻮﺑ .

د ﯾ ﺘﺑﺎ ﯽ ندﺮﮐ

ﺎﻘﻟا ي د ﯾ ﺖﺑﺎ ﺎﺑ رﺰﺗ ﯾ ﻖ ﻞﺧاد ﻗﺎﻔﺻ ﯽ

mg/kg

50 دﺎﻣ ة

STZ

) ﺖﮐﺮﺷ

Enzo

ﺮﻣآ ﯾ ﺎﮑ ( تر رد ﺎـﻫ ي هوﺮـﮔ ﺎـﻫ ي لﺮـﺘﻨﮐ د ـﯾ ﺘﺑﺎ ﯽ ، ةرﺎـﺼﻋ ،ﻪـﻧﺰﮔ

ﻣرﻮﻔﺘﻣ ﯿ

،ﻦ ﺮﻤﺗ ﯾ

،ﻦ ﺮﻤﺗ+ﻪﻧﺰﮔ ﯾ ﻦ ﻣرﻮﻔﺘﻣ و ﯿ ﻦ ﺮﻤﺗ+

ﯾ ﻦ .ﺖﻓﺮﮔ مﺎﺠﻧا 48

ﺎﺗ 72 رﺰﺗ زا ﺲﭘ ﺖﻋﺎﺳ ﯾ

،ﻖ نﻮﺧ ﮔﯿ ﺮ ي تر مد زا ﺎﻫ و ﺖـﻓﺮﮔ مﺎـﺠﻧا

ﺎﻬﻧآ ﯽﯾ ﻪﮐ ﻣﯿ ناﺰ ﮔ ﻻﺎﺑ ﺎﺘﺷﺎﻧ نﻮﺧ ﺰﮐﻮﻠ ي

250 ﻣ ﯿﻠ ﯽ مﺮﮔ ﺳد رد ﯽ ﻟﯿ ﺮﺘ ﺪﻨﺘﺷاد

، دﯾ ﺘﺑﺎ ﯽ ﻘﻠﺗ ﯽ ﺪﺷ ﺪﻧ

.

(7)

73

شور ﻬﺗ ﮥﯿ ةرﺎﺼﻋ ﺑآ ﯽ ﮔ ﯿ هﺎ ﻪﻧﺰﮔ

راﺪﻘﻣ ي گﺮﺑ و ﻪﻗﺎﺳ زا ﮔﯿ

هﺎ ﮏﭼﻮﮐ تﺎﻌﻄﻗ ﻪﺑ شﺮﺑ زا ﺲﭘ ﻪﻧﺰﮔ ﻊﻤﺟ

روآ ي ﺖﺴﺷ و و اﻮﻫ رد ﺲﭙﺳ .ﺪﺷ هداد ﻮﺷ ي

ﺎـﺑ و ﺪـﺷ ﮏـﺸﺧ دازآ

ﻦﮐدﺮﺧ هﺎﮕﺘﺳد ﻪﺑ

ترﻮﺻ ردﻮﭘ ﺪﻣآرد ﺲﭙﺳ . 60 مﺮﮔ ردﻮﭘ ﮔﯿ هﺎ ﺮﺸﺑ ﻞﺧاد ﻪﻧﺰﮔ 5/

2 ﻟﯿ ﺮﺘ ي ود و ﺪﺷ هداد راﺮﻗ ﻟﯿ

ﺮﺘ ) ﺮﻄﻘﻣ بآ د

ـﯾ ﻧﻮ ﯿ هﺰ ﻪـﺑ (

ور ﺮﺸﺑ و ﻪﻓﺎﺿا نآ ي

ﻫ ﯿ ﺮﺘ صﻮﺼﺨﻣ )

لﺪﻣ

MR3001 K

ﺖﮐﺮﺷ

Heidolph

نﺎﻤﻟآ ( تراﺮﺣ ﺎﺑ ﻼﻣ

ﯾ ﻢ هداد راﺮﻗ ﺎـﺑ ،نﺪﻧﺎﺷﻮﺟ زا ﺲﭘ .ﺪﺷ

ﺬﻏﺎﮐ ﻓﺎﺻ ﯽ ﺪﻧﺎﺷﻮﺟ ة ﺮﻈﻧدرﻮﻣ ﻔﺼﺗ ﯿﻪ .ﺪﺷ هرﺎﺼﻋ ﮔﯿ ﺮ ي هﺎﮕﺘﺳد ﻞﺧاد ﻄﻘﺗ

ﯿﺮ رد ﻸﺧ راود ) رﺎـﺗور ي

Labratory 4003

ﺖﮐﺮـﺷ ﺖﺧﺎـﺳ ،

Heidolph

نﺎﻤﻟآ ، ( ﺎﻣد ﺎﺑ ي 45 ﺟرد ﮥ ﺘﻧﺎﺳ ﯽ داﺮﮔ رﺎﺸﻓ و ﻸﺧ 65

mbar

رود و 20 هداد راﺮﻗrpm

اﺮﺑ .ﺪﺷ ي ﻬﺗ ﯿﮥ ،لﻮﻠﺤﻣ ةرﺎﺼﻋ ﺑآ ﯽ ﮔ ﯿ هﺎ

ﻞﺣ ﺮﻄﻘﻣ بآ رد ﻪﻧﺰﮔ هﺪﺷ

اﺮﺑ و ي و دﻮﺷ ﻞﺣ ًﻼﻣﺎﮐ ﻪﮑﻧآ ﻟﻮﻠﺤﻣ

ﯽ ﻗر ﯿ ﻖ فﺎﺻ و ﻪﺑﻼﻣ ﺖﺳد آﯾ ﺪ،

ﻞﺧاد ﻟﻮﻟ ﮥ ور و نﻮﮑﻟﺎﻓ ي

راﺮـﻗ ﺲـﮑﺗرو

ﺪﺷ هداد

، ﻪﺑ ﻮﺤﻧ ي لﻮﻠﺤﻣ ﻪﮐ ﻪﺑ

ﺖﺳد هﺪﻣآ ﻪﺑ ﺘﺣار ﯽ ﮓﻧﺮﺳ زا ﻟﻮﺴﻧا

ﯿ ﻦ اﺮﺑ ،ﺪﻨﮐ رﻮﺒﻋ ي

ﻬﺗ ﯿﮥ ةرﺎﺼﻋ درﻮﻣ ﻧﯿ زﺎ ﻻﺎﺑ ﻞﺣاﺮﻣ ،حﺮﻃ ﺪﻨﭼ

ﯾ ﻦ رﺎـﺑ

ﺪﺷ راﺮﮑﺗ ) 24 .(

تﻼﺧاﺪﻣ ﺬﻐﺗ

ﯾﻪ ا ي ﺮﻤﺗ و ﯾ ﻦ ﺎﻨﺷ

هوﺮﮔ ﺎﻫ ي ﻪﻧﺰﮔ ﻪﻧازور 25 /1 ﻣ ﯿﻠ ﯽ ﮐ ﺮﻫ ﺮﺑ مﺮﮔ ﯿ

مﺮﮔﻮﻠ ﺑ نزو زا دﻮﺧ نﺪ ةرﺎﺼﻋ ﺑآ ﯽ ﮔ ﯿ هﺎ هوﺮﮔ و ﻪﻧﺰﮔ ﺎﻫ

ي ﻣرﻮﻔﺘﻣ ﯿ ﻦ 100 ﻣﯿ ﻠ ﯽ ﺮـﻫ ﺮـﺑ مﺮﮔ

ﮐﯿ مﺮﮔﻮﻠ دﻮﺧ نﺪﺑ نزو زا تﺪﻣ ﻪﺑ ار نآ لﻮﻠﺤﻣ

ﻪﺘﻔﻫ رﺎﻬﭼ زا

ﺮﻃ ﯾ ﻖ ﻨﮑﺗ ﻂﺳﻮﺗ ژاوﺎﮔ ﯿ

ﺴ ﯿ ﻦ ﺎﻣزآ ﯾ هﺎﮕﺸ ﻪـﻧ ﺖﻋﺎـﺳ) ﺎـﺗ

(ﺢﺒـﺻ هد رد

ﯾ ﺖـﻓﺎ

ﺪﻧدﺮﮐ ؛ ﻨﭽﻤﻫ ﯿ ﻦ هوﺮﮔ ﺎﻫ ي ﺮﻤﺗ ﯾ ﻦ ﺮﻤﺗ مﺎﺠﻧا ﻪﺑ ﯾ

ﻦ زاﻮﻫ ي .ﺪﻨﺘﺧادﺮﭘ ﺎﻨﺷ ياﺮﺑ

زﺎﺳ رﺎﮔ ي تر ﺎﻫ ﺑﺎ ﺮﻤﺗ ﯾ ﻦ ،ﺎﻨﺷ ـﯾ ﮏ ﻪـﺘﻔﻫ ﺶﯿـﭘ عوﺮـﺷ زا

ﺮﻤﺗ ﻞﮑﺗوﺮﭘ ﯾ

،ﻦ هوﺮﮔ ﺎﻫ ي ﺮﻤﺗ ﯾ ﻦ تﺪﻣ ﻪﺑ هوﺮﮔ ﺮﻫ و ﻪﻧﺎﮔاﺪﺟ ﺞﻨﭘ

ﻗد ﯿ ﻪﻘ رد ﻪﺳ و ،ﻪﺘﻔﻫ لوا زور 10

ﻗد ﯿ ﻪﻘ رد ﻪﺳ ﺮﺨﺘﺳا رد ﻪﺘﻔﻫ مود زور

ﺎﻨﺷ ي دﺎﻌﺑا ﺎﺑ) نﺎﮔﺪﻧﻮﺟ صﻮﺼﺨﻣ 75

× 75

× 53 ﺘﻧﺎﺳ ﯽ ﺮﺘﻣ ﺎﻣد ﺎﺑ ي بآ 30 رﺎـﺑ ﻪﻓﺎﺿا .ﺪﻧدﺮﮐ ﺎﻨﺷ (ﻪﺟرد ﺮﻤﺗ

ﯾ ـﻨ ﯽ ﺮﻃ زا ـﯾ ﻖ ـﻈﻨﺗ ﯿ ﻢ ﺎـﺿا ﻪﻓ

ﺮﮐ ند تر مد ﻪﺑ ﻪﻧزو ﺎﻫ

ظﺎﺤﻟ ﺎﻨﺷ نﺎﻣز تﺪﻣ و ﺮﺨﺘﺳا بآ ﻖﻤﻋ ، ﺪﺷ

ﺑ هوﺮﮔ ﺮﻫ . ﻪ

ﻪﻧﺎﮔاﺪﺟ ترﻮﺻ ﻪﭽﺿﻮﺣ رد

ﻣﺎـﻤﺗ .ﺪﻧدﺮﮐ ﺎﻨﺷ ﯽ

ﻞـﺣاﺮﻣ

ﺮﻤﺗ ﯾ ﻦ ﻨﮑﺗ ﮏﻤﮐ ﺎﺑ و ﺮﮕﺸﻫوﮋﭘ ﻂﺳﻮﺗ ﯿ

ﺴ ﯿ ﻦ ﺎﻣزآ ﯾ هﺎﮕﺸ مﺎﺠﻧا ﺖﻓﺮﮔ لﻮﻃ . ةرود ﺮﻤﺗ ﯾ ﻦ ﺎﻨﺷ ﻪـﺘﻔﻫ رﺎﻬﭼ .دﻮـﺑ ﻪـﺘﻔﻫ رد زور ﺞﻨـﭘ و

ـﻤﺗ ﺮﯾ ﻦ

تر ﺎﻫ ﺘﻔﻫ رد ﮥ لوا 15 ﺎﺗ 20 ﻗد ﯿ ﻪﻘ ندﺮﮐ ﺎﻨﺷ ﻖﻤﻋ ﺎﺑ ﺮﺨﺘﺳا رد

30 ﺘﻧﺎﺳ ﯽ ﺮﺘﻣ ﺘﻔﻫ ،بآ ﮥ مود 25 ﺎـﺗ 35 ﻗد ﯿ ﻪـﻘ ﻟﺎﻌﻓ ـﯿ ﺖ بآ ﻖـﻤﻋ و ﺎﻨـﺷ

ﺮﺨﺘﺳا 45 ﺘﻧﺎﺳ ﯽ

،ﺮﺘﻣ ﺘﻔﻫ ﮥ مﻮﺳ 30 ﺎﺗ 45 ﻗد ﯿ ﻪﻘ ﻟﺎﻌﻓ ﯿ ﺖ ﺎﺑ هاﺮﻤﻫ ﺎﻨﺷ ﻧزو

ﮥ 10 ﻣﺮﮔ ﯽ ) 5 ﺎﺗ 10 (نﺪﺑ نزو ﺪﺻرد ﻞـﺻو

رد تر مد ﻪـﺑ هﺪـﺷ

ﻖﻤﻋ ﺎﺑ ﺮﺨﺘﺳا 50

ﺘﻧﺎﺳ ﯽ ﺮﺘﻣ و ﺘﻔﻫ ﮥ ﺎﻬﭼ مر ﻟﺎﻌﻓ نﺎﻣز ﯿ ﺖ 45 - 50 ﻗد ﯿ ﻪﻘ ندﺮﮐ ﺎﻨﺷ ﺖﻣوﺎﻘﻣ ندﺮﮐ ﻞﺻو ﺎﺑ هاﺮﻤﻫ

20 ﺎﺗ 25 ـﻣﺮﮔ ﯽ ) 10 ﺎـﺗ

15 تر مد ﻪﺑ (نﺪﺑ نزو ﺪﺻرد ﺎﻫ

ﻖﻤﻋ ﺎﺑ ﺮﺨﺘﺳا رد 50

ﺘﻧﺎﺳ ﯽ ﺮﺘﻣ اﺰﻓا ؛ﺖﻓﺮﮔ مﺎﺠﻧا ﯾ

ﺶ اﺰـﻓا و ندﺮـﮐ ﺎﻨـﺷ نﺎـﻣز تﺪـﻣ ﯾ

ﺶ و بآ ﻖـﻤﻋ

ﻨﭽﻤﻫ ﯿ ﻦ رد ﺖﻣوﺎﻘﻣ زا هدﺎﻔﺘﺳا ﺘﻔﻫ

ﮥ مﻮﺳ ﺎﻋر ﺖﻬﺟ ﯾ ﺖ ﻪﻓﺎﺿا ﻞﺻا رﺎﺑ

رﺪﺗ ﯾ ﺠ ﯽ دﻮﺑ ) 24 .(

شور ﻊﻤﺟ روآ ي ﻟﺎﻧآ و ﯿ ﺰ ﻧﻮﻤﻧ ﮥ ﺖﻓﺎﺑ و نﻮﺧ

48 ﺮﺧآ زا ﺲﭘ ﺖﻋﺎﺳ ﯾ

ﻦ ﺴﻠﺟ ﮥ ﺮﻤﺗ ﯾ

،ﻦ تر ﺎﻫ وزاﺮﺗ ﻂﺳﻮﺗ ي

د ﯾ ﺠ ﯿ لﺎﺘ ) ﺎﮑﺳ نﺎﻤﻟآ ( نزو ﺸﮐ ﯽ ﮐﺮﺗ ﻂﺳﻮﺗ ﺎﺘﺷﺎﻧ ﺖﻟﺎﺣ رد و هﺪﺷ ﯿ

ﺐ ﻣﺎﺘﮐ ﯿ ﻦ -

ازﯾ ﺳﻼ ﯿ ﻦ ﺑ ﯽ شﻮﻫ زا ﺲﭘ .ﺪﻧﺪﺷ ﺄﺗ

ﯿﯾ ﺪ ﺑ ﯽ ﺷﻮﻫ ﯽ

، ﻪﻧﻮﻤﻧ ﻪﻠﺻﺎﻓﻼﺑ ﻫﺎ

ي ﻧﻮﺧ ﯽ ﻪﺑ رﻮﻈﻨﻣ هزاﺪﻧا ﮔ ـﯿ ﺮ ي آ ،ﺰﮐﻮـﻠﮔ ﺢﻄـﺳ ﯾﺮ

ـﯾ ﺰﯾ ﻦ و

FGF21

زا

ﺣ ﺐﻠﻗ ﯿ تﺎﻧاﻮ ﻊﻤﺟ روآ ي زا ﺲﭘ .ﺪﺷ نﻮﺧ

ﮔﯿ ﺮ ي

، ﺖﻓﺎﺑ ﺎﻫ ي ﻠﻀﻋ ﮥ ﺑﺮﭼ و نار ﺖﺸﭘ ﯽ

ﺎﺸﺣا ﯽﯾ ﺮﺘﻧاﺰﻣ فاﺮﻃا رد) ﯾﺎ

هدور ﺪﻨﺑ ﻔـﺳ و ( ﯿﺪ ـﺑﺮﭼ) ﯽ

ﻔﺳ ﯿﺪ ز ﯾﺮ رد ﺖﺳﻮﭘ ﺣﺎﻧ

ﯿﮥ ﻟﺎﺸﮐ ﮥ ﺖﺴﺷ زا ﺲﭘ هوﺮﮔ ﺮﻫ (نار و

ﻓ مﺮﺳ رد ﻮﺷ ﯿﺰ

ﯾ ژﻮﻟﻮ ﯾ ﮏ ﺮﺑا ي ﺎﻣزآ مﺎﺠﻧا ﯾ

ﺶ ﺎﻫ ي ﻟﻮﮑﻟﻮﻣ ﯽ )ﺑ ـﯿ نﺎ نژ ﺎـﻫ ي

PGC-1α

و

UCP-1

(ﺑ ﻪ ﻞﺧاد رد ﻪﻧﺎﮔاﺪﺟ ترﻮﺻ ﻟﻮﻟ

ﮥ نﻮﮑﻟﺎﻓ ﺎﻫ ي 5 /1 ﻣ ﯿﻠ ﯽ ﻟﯿ ﺮﺘ ي ﻧ ﮏﻧﺎﺗ ﻞﺧاد رد و هﺪﺷ هداد راﺮﻗ ﯿ

نژوﺮﺘ رد و ﻪﺘـﺷاﺬﮔ

ﺎﻣد ي ﯽﻔﻨﻣ 80 ﯽﺘﻧﺎﺳ ﮥﺟرد داﺮﮔ

راﺪﻬﮕﻧ ي ﻣﺮﺳ ﺰﮐﻮﻠﮔ ﺖﻈﻠﻏ .ﺪﺷ ﯽ

ﺴﮐا ﺰﮐﻮﻠﮔ شور ﻪﺑ ﯿ

زاﺪ ﮐ زا هدﺎﻔﺘﺳا ﺎﺑ ﯿ

ﺖ ) سرﺎﭘ ا ﺖﺧﺎﺳ نﻮﻣزآ ﯾ

ناﺮ (

هزاﺪﻧا ﮔﯿ ﺮ ي ﺪﺷ

.

ياﺮﺑ زﺎﺳاﺪﺟ ي زاRNA

ﺖﻓﺎﺑ ﺑﺮﭼ ﯽ ﻔﺳ ﯿﺪ ز ﯾ ﺘﺳﻮﭘﺮ ﯽ و ﺑﺮﭼ ﯽ هﻮﻬﻗ ا ي

، اﺮﺗ ـﯾ لوﺰ ) ﺖﮐﺮـﺷ

Scientific Thermo

( ﻖﺑﺎـﻄﻣ ﻞﻤﻌﻟارﻮﺘـﺳد

ﺖﮐﺮﺷ هﺪﻧزﺎﺳ هدﺎﻔﺘﺳا و ﺪﺷ ﺲﭙﺳ

RNA

جاﺮﺨﺘﺳا هﺪﺷ ﺎﺑ هدﺎﻔﺘﺳا زا ﮐﯿ ﺖ

Scientific DNaseI Thermo

زا ﻪﻧﻮﮔﺮﻫ ﮔدﻮﻟآ ﯽ ﻪﺑ

DNA

و

ﺰﻧآ ﯾ ﻢ ﺎﻫ ي ﺮﺨﺗ ﯾ ﺐ ﺪﻨﻨﮐ ة ﻻﺎﭘRNA

ﯾ ﺶ زﺎﺴﮐﺎﭘ و ي زا .ﺪﺷ ﺮﻫ ماﺪﮐ زا ﻪﻧﻮﻤﻧ ﺎﻫ

، 2 ﻣﯿ مﺮﮔوﺮﮑ

total RNA

اﺮﺑ ي ﺰﺘﻨﺳ ﻟوا ﯿ ﻦ ﺘﺷر ﮥ

cDNA

(8)

74 نﺎﺘﺴﻣز ،مرﺎﻬﭼ ةرﺎﻤﺷ ،ﻢﻫدرﺎﻬﭼ ةرود ،ﯽﺷزرو ﯽﺘﺴﯾز مﻮﻠﻋ

1401

هدﺎﻔﺘﺳا ﺪﺷ . اﺮﺑ ي ﺰﺘﻨﺳ

cDNA

زا ﮐﯿ ﺖ زﺎﺘﻨﻣﺮﻓ هدﺎﻔﺘﺳا ﺪﺷ . ﻪﺑ اﯾ ﻦ ترﻮﺻ ﻪﮐ اﺮﺑ ي ﺖﺧﺎﺳ

cDNA

زا ﺮﮔزﺎـﻏآ اﺮﭘ ﯾ ﺮـﻤ ) موﺪـﻧر ﺮـﻣاﺰﮕﻫ (

هدﺎﻔﺘﺳا ﺷ ﺪ

.

آﯾ ﺮﯾ ﺰﯾ ﻦ ﻧﯿ ﺰ ﻻا شور ﻪـﺑ ـﯾ

اﺰ ) ﮐ ـﯿ ﺖ

Rat Irisin Elisa Kit, Shanghai Cryctal Day Biotech Co, LTD; cat. No: E6281Ra

(

هزاﺪﻧا ﮔﯿ ﺮ ي ﺨﺸﺗ دوﺪﺣ .ﺪﺷ ﯿ

ﺼ ﯽ اﺮﺑ ي اﯾ ﻦ ﮐ ﯿ ﺖ 05 /0 ﺎﺗ 30 مﺮﮔﻮﻧﺎﻧ رد ﻣﯿ ﻠ ﯽ ﻟﯿ ﺮﺘ ﺳﺎﺴﺣ و ﯿ ﺖ نآ 03 /0 مﺮﮔﻮﻧﺎﻧ رد ﻣﯿ ﻠ ﯽ ﻟﯿ ﺮـﺘ ﺮـﺿ .دﻮـﺑ ﯾ

ﺐ

ﻐﺗ ﯿﯿ تاﺮ ﻣ ﯿ نﺎ ﺮﻓ و ﻣزآا ﻧﻮ ﯽ ﻪﺑ ﺗﺮﺗ ﯿ ﺐ زا ﺮﺘﻤﮐ 10 و 8 ﺪﺻرد ﻣ .دﻮﺑ ﯿ ناﺰ ﻣﺮﺳ ﺖﻈﻠﻏ ﯽ

FGF21

ﮐ زا هدﺎﻔﺘـﺳا ﺎﺑ ـﯿ

ﺖ ﻻا ـﯾ اﺰ

(Crystalday

Biotech, China)

ﻻا هﺎﮕﺘﺳد و ﯾ

اﺰ ر ﯾ رﺪ )

Hyperion

، لﺪﻣ

MPR+4

( ﻨﭽﻤﻫ .ﺪﺷ ﺶﺠﻨﺳ ﯿ

ﻦ ﻣ ﯿ ناﺰ ﺑﯿ نﺎ نژ

PGC-1α

ﻪﻠﻀﻋ ﺖﻓﺎﺑ رد

ﺑ و ﯿ نﺎ نژ

UCP-1

ﻔﺳ ﯿﺪ و هﻮﻬﻗ ا ي ﺑ شور ﻪ

Real-time PCR

و ﻪﺑ ترﻮﺻ ﻤﮐ ﯽ ﺠﻨـﺳ ﯿ هﺪ اﺮﭘ .ﺪـﺷ ﯾ ﺎـﻫﺮﻤ ي ـﺻﺎﺼﺘﺧا ﯽ

نژ ﺎﻫ ي ﺮﻈﻧدرﻮﻣ ﻟاﻮﺗ زا هدﺎﻔﺘﺳا ﺎﺑ ﯽ

ﺎﺳ زا نژ ﯾ ﺖ

www.genenames.org

و مﺮﻧ راﺰﻓا

generunner

ـﺣاﺮﻃ ﯽ ـﺻﺎﺼﺘﺧا و ﯿ

ﺖ ﺮﻃ زا ـﯾ ﻖ

ﻧﺎﻣﺎﺳ ﮥ اﺮﭘ ﯾ ﺮﻤ ﺖﺳﻼﺑ ﺎﭘﯾ هﺎﮕ هداد

ncbi.org

ﺄﺗ ﯿﯾ ﺪ ﺪﺷ . ياﺮﺑ ﺳرﺮﺑ ﯽ ﺑﯿ نﺎ نژ ﺎﻫ لوﺪﺟ) 1 وﺮﮔ رد ( ه ﺎﻫ ي درﻮـﻣ ﯽـﺳرﺮﺑ ﻨﮑﺗ زا ، ـﯿ ﮏ

Real

Time PCR

ﻤﮐ ﯽ ﺎﺳ شور ﻪﺑ ﯾ

ﺮﮔﺮﺒ ﯾ ﻦ .ﺪﺷ هدﺎﻔﺘﺳا اﺮﺑ

ي مﺎﺠﻧا

Real time PCR

، ﺎﺑﯾ ﺪ ﺮﺘﻬﺑ ﯾ ﻦ ﺖﻈﻠﻏ

cDNA

دروآﺮﺑ دﻮـﺷ

، اﺮـﺑ ي ا ـﯾ ﻦ

رﻮﻈﻨﻣ ﺖﻈﻠﻏ ﺎﻫ ي ﻒﻠﺘﺨﻣ

cDNA

ﺳرﺮﺑ ﯽ و رد ﺎﻬﻧ ﯾ ﺖ ﺖﻈﻠﻏ ﺐﺳﺎﻨﻣ اﺮﺑ ي

Real time PCR

رد ﺮﻈﻧ ﻪﺘﻓﺮﮔ ﺪﺷ ﻧاﺮـﻓر لﺮـﺘﻨﮐ نژ . ﺲ

رد

اﯾ ﻦ ﺘﮐا ﺎﺘﺑ ﺖﺴﺗ ﯿ

ﻦ

(ß Actin)

ﺑ و ﺪﺷ ﻪﺘﻓﺮﮔ ﺮﻈﻧ رد ﯿ

نﺎ ژ ن ﺎﻫ ي ﺮﻈﻧدرﻮﻣ شور ﺎﺑ

Δ CT

ﺪﻧﺪﺷ ﻪﺒﺳﺎﺤﻣ

.

لوﺪﺟ 1 ﻟاﻮﺗ . ﯽ ﺎﻫ نژ ﺮﻤﯾاﺮﭘ ي ﺎﻫ

تر ي ﺶﺠﻨﺳ رد ﻪﮐ RT-PCR

ﯽﺳرﺮﺑ هﺪﺷ ﺪﻧا .

Gene Forward (5’-3’) Reverse (5’-3’)

PGC-1α 5’-CCAAAGGATGCGCTCTCGTTCA-3’ 5’-CGGTGTCTGTAGTGGCTTGACT-3’

UCP-1 5’-TGGAATAGCGGCGTGCTTG-3’ 5’-CTCATCAGATTGGGAGTAG-3’

Β-Actin 5’-CACCATTGGCAATGAGCGGTTC-3’ 5’-AGGTCTTTGCGGATGTCCACGT-3’

شور ﺎﻫ ي رﺎﻣآ ي

ﻪﺑ رﻮﻈﻨﻣ ﺳرﺮﺑ ﯽ ﺒﻃ ﯿﻌ ﯽ ندﻮﺑ زﻮﺗ ﯾﻊ هداد ﺎﻫ ﺲﻧﺎﺠﺗ دﻮﺟو و راو

ﯾ ﺲﻧﺎ ﻪﺑ ﺗﺮﺗ ﯿ ﺐ زا نﻮﻣزآ ﺎﻫ ي ﭘﺎﺷ ﯿ وﺮ وﯾ ﮏ ﻮﻟ و ﯾ ﻦ .ﺪﺷ هدﺎﻔﺘﺳا رﺎـﻣآ نﻮﻣزآ

ي

ﻠﺤﺗ ﯿ ﻞ راو ﯾ ﺲﻧﺎ ﯾ ﻪﻓﺮﻄﮑ نﻮﻣزآ و ﻘﻌﺗ ﯿﺒ ﯽ ﯽﮐﻮﺗ اﺮﺑ ي ﺎﻘﻣ ﯾ ﺴ ﮥ ﻐﺘﻣ ﯿ ﺎﻫﺮ ي هوﺮﮔ ﺎـﻫ رد ﺢﻄـﺳ ـﻨﻌﻣ ﺎ راد ي 05 /0

= تﺎـﻋﻼﻃا .ﺪـﺷ هدﺎﻔﺘـﺳاP

ﻪﺑ ﺖﺳد هﺪﻣآ ﻔﺘﺳا ﺎﺑ هدﺎ زا مﺮﻧ راﺰﻓا وSPSS ﯾ اﺮ ﯾ ﺶ 20 ﺰﺠﺗ ﯾﻪ ﻠﺤﺗو ﯿ ﻞ ﺪﺷ .

ﯾ ﻪﺘﻓﺎ ﺎﻫ ي ﺶﻫوﮋﭘ

زا ﺲﭘ ﺶﻫوﮋﭘ ﺞﯾﺎﺘﻧ داد نﺎﺸﻧ ﻪﻠﺧاﺪﻣ

نزو ﻦﯿﮕﻧﺎﯿﻣ ﺮﯾﺎﺳ

هوﺮﮔ ﺎﻫ ﺖﺒﺴﻧ ﻢﻟﺎﺳ لﺮﺘﻨﮐ هوﺮﮔ ﻪﺑ ﻪﺑ

رﻮﻃ يرادﺎﻨﻌﻣ دﻮﺑ ﺮﺘﻤﮐ

) 05 / 0

= (P

رد .

اﯾ ﻦ درﻮﻣ ﺮﯾدﺎﻘﻣ ﻦﯾﺮﺘﻤﮐ طﻮﺑﺮﻣ نزو

لﺮﺘﻨﮐ هوﺮﮔ ﻪﺑ دﯾ

ﺘﺑﺎ ﯽ .دﻮﺑ ﺳرﺮﺑ ﯽ توﺎﻔﺗ ﻧزو ﯽ ﺑﯿ ﻦ هوﺮﮔ ﺎﻫ ي ﺗﯿ رﺎﻤ ﻪﺘﻔﻫ رﺎﻬﭼ زا ﺲﭘ ﻘﺤﺗ

ﯿ ﻖ نﺎﺸﻧ

ﻪﮐ داد نزو ﻦﯿﮕﻧﺎﯿﻣ هوﺮﮔ

ﺎﻫ ي ﻔﺘﻣ ﻣرﻮ ﯿ ﻦ + ﺮﻤﺗ ﯾ ﻦ ) 008 / 0

= ( P

و ﻣرﻮﻔﺘﻣ ﯿ ﻦ ) 01 /0

= ( P

ﻪﺑ ﺖﺒﺴﻧ لﺮﺘﻨﮐ هوﺮﮔ

دﯾ ﺘﺑﺎ ﯽ ﻪﺑ رﻮﻃ يرادﺎﻨﻌﻣ

ﺮﺘﺸﯿﺑ دﻮﺑ ﻞﮑﺷ) 1 - ﻒﻟا (.

ﺰﮐﻮﻠﮔ ياﺮﺑ ﻪﻓﺮﻄﮑﯾ ﺲﻧﺎﯾراو ﻞﯿﻠﺤﺗ نﻮﻣزآ ﺞﯾﺎﺘﻧ )

001 /0 ،P=

74 / 11

= ( F

ﺰﮐﻮﻠﮔ ﺖﻈﻠﻏ ﻦﯿﮕﻧﺎﯿﻣ ﻦﯿﺑ ﻪﮐ داد نﺎﺸﻧ هوﺮﮔ

ﺎﻫ توﺎﻔﺗ

ﻘﻌﺗ نﻮﻣزآ ﺞﯾﺎﺘﻧ .دراد دﻮﺟو يرادﺎﻨﻌﻣ ﻪﮐ داد نﺎﺸﻧ ﯽﻧوﺮﻔﻧﻮﺑ ﯽﺒﯿ

ﻦﯿﮕﻧﺎﯿﻣ ﺖﻈﻠﻏ ﺰﮐﻮﻠﮔ نﻮﺧ ﻤﻫ ﮥ هوﺮﮔ ﺎﻫ ي ﺗﯿ رﺎﻤ رد ﺎﻘﻣ ﯾ ﻪﺴ هوﺮﮔ ﺎﺑ

لﺮﺘﻨﮐ دﯾ ﺘﺑﺎ ﯽ ﻪﺑ رﻮﻃ يرادﺎﻨﻌﻣ دﻮﺑ ﺮﺘﻤﮐ

) 001 /0

= .(P

ﺎﺑ ﻪﺴﯾﺎﻘﻣ مﺎﮕﻨﻫ لﺮﺘﻨﮐ هوﺮﮔ

دﯾ ﺘﺑﺎ ﯽ

، ﻏ ﺖﻈﻠ ﺰﮐﻮﻠﮔ هوﺮﮔ نﻮﺧ ﻣرﻮﻔﺘﻣ

ﯿ ﻦ هوﺮﮔ و

ﻪﻧﺰﮔ ﺮﻤﺗ+

ﯾ ﻦ ﺖﺒﺴﻧ ، ﻪﺑ دﯾ ﺮﮕ هوﺮﮔ ﺎﻫ ي ﻘﺤﺗ ﯿ ﻖ ياراد ، ﻤﮐ ﺮﯾدﺎﻘﻣ ﻦﯾﺮﺘ ﺰﮐﻮﻠﮔ

نﻮﺧ ﺪﻧدﻮﺑ ) 001 /0

= ( P

ﻞﮑﺷ) 1 - ب توﺎﻔﺗ ،لﺎﺣ ﺮﻫ ﻪﺑ .(

ﺖﻈﻠﻏ ﻦﯿﮕﻧﺎﯿﻣ رد يرادﺎﻨﻌﻣ

FGF21

هوﺮﮔ ﺎﻫ هﺪﻫﺎﺸﻣ ﻧ ﺪﺸ ) 526 /0 ،P=

87 /0

= ( F

ﻞﮑﺷ) 1 - ج .(

(9)

75

ﻦﯾﺰﯾﺮﯾآ ياﺮﺑ ﻪﻓﺮﻄﮑﯾ ﺲﻧﺎﯾراو ﻞﯿﻠﺤﺗ نﻮﻣزآ ﺞﯾﺎﺘﻧ )

001 /0 ،P=

08 / 19

= ( F

ﻦﯾﺰﯾﺮﯾآ ﺖﻈﻠﻏ ﻦﯿﮕﻧﺎﯿﻣ ﻦﯿﺑ ﻪﮐ داد نﺎﺸﻧ هوﺮﮔ

ﺎﻫ ﺗ توﺎﻔ

ﻦﯾﺰﯾﺮﯾآ ﺖﻈﻠﻏ ﻦﯿﮕﻧﺎﯿﻣ ﻪﮐ داد نﺎﺸﻧ ﯽﻧوﺮﻔﻨﺑ ﯽﺒﯿﻘﻌﺗ نﻮﻣزآ ﺞﯾﺎﺘﻧ .دراد دﻮﺟو يرادﺎﻨﻌﻣ ﻤﻫ

ﮥ هوﺮﮔ ﺎﻫ ﯽﺘﺑﺎﯾد لﺮﺘﻨﮐ هوﺮﮔ ﻪﺑ ﺖﺒﺴﻧ ﻪﺑ

رﻮﻃ

دﻮﺑ ﺮﺘﺸﯿﺑ يرادﺎﻨﻌﻣ )

001 / 0

= ( P

ﯽﺘﺑﺎﯾد لﺮﺘﻨﮐ هوﺮﮔ ﻪﺑ ﺖﺒﺴﻧ ﻢﻟﺎﺳ لﺮﺘﻨﮐ هوﺮﮔ ﻦﯾﺰﯾﺮﯾآ ﺖﻈﻠﻏ ﻦﯿﮕﻧﺎﯿﻣ ﻪﺑ

رﻮﻃ دﻮﺑ ﺮﺗﻻﺎﺑ يرادﺎﻨﻌﻣ

) 001 /0

= ( P

ﻞﮑﺷ) 1 - د .(

رد صﻮﺼﺧ نژ نﺎﯿﺑ

UCP-1

ﻮﻧآ نﻮﻣزآ ﺞﯾﺎﺘﻧ و

ا ) 001 /0 ،P=

16 / 51

= ( F

نژ نﺎﯿﺑ ﻦﯿﺑ ﻪﮐ داد نﺎﺸﻧ

UCP-1

هوﺮﮔ ﺎﻫ توﺎﻔﺗ

.دراد دﻮﺟو يرادﺎﻨﻌﻣ ﺎﺘﻧ

ﯾ ﺞ نﻮﻣزآ ﻘﻌﺗ ﯿﺒ ﯽ ،داد نﺎﺸﻧ ﯽﻧوﺮﻔﻨﺑ ﻣﯿ

ناﺰ نژ نﺎﯿﺑ

UCP-1

ﯽﺘﺑﺎﯾد لﺮﺘﻨﮐ ﻪﺑ ﺖﺒﺴﻧ ﻢﻟﺎﺳ لﺮﺘﻨﮐ هوﺮﮔ رد ﻪﺑ

رﻮﻃ

ﺎﻨﻌﻣ ﺘﺸﯿﺑ يراد دﻮﺑ ﺮ

) 001 /0 ﺮﮕﯾد زا ﺮﺘﺸﯿﺑ ﻢﻟﺎﺳ لﺮﺘﻨﮐ هوﺮﮔ رد نژ ﻦﯾا نﺎﯿﺑ ناﺰﯿﻣ ﻦﯿﻨﭽﻤﻫ ،(P=

هوﺮﮔ ﺎﻫ ي دﻮﺑ نﺎﻣرد )

001 /0 .(P=

ﺑ ﯽﺒﯿﻘﻌﺗ نﻮﻣزآ ﺞﯾﺎﺘﻧ ﻦﯿﻨﭽﻤﻫ ﺮﻔﻨ

ناﺰﯿﻣ ﻪﮐ داد نﺎﺸﻧ ﯽﻧو

UCP-1

ﺮﮕﯾد ﻪﺑ ﺖﺒﺴﻧ ﯽﺘﺑﺎﯾد لﺮﺘﻨﮐ هوﺮﮔ هوﺮﮔ

ﺎﻫ ي ﺶﻫوﮋﭘ درﻮﻣ ﻪﺑ

رﻮﻃ

دﻮﺑ ﺮﺘﻤﮐ يرادﺎﻨﻌﻣ )

001 /0 ﻞﮑﺷ) (P=

1 - ه .(

رد صﻮﺼﺧ نژ نﺎﯿﺑ

PGC1-α

نﻮﻣزآ ﺞﯾﺎﺘﻧ اوﻮﻧآ

) 001 /0 ،P=

13 / 10

= نژ نﺎﯿﺑ ﻦﯿﺑ ﻪﮐ داد نﺎﺸﻧ F PGC1-α

هوﺮﮔ ﺎﻫ توﺎﻔﺗ

.دراد دﻮﺟو يرادﺎﻨﻌﻣ ﺎﺘﻧ

ﯾ ﺞ نﻮﻣزآ ﻘﻌﺗ ﯿﺒ ﯽ ﻔﻨﺑ ﯽﻧوﺮ داد نﺎﺸﻧ ﻣﯿ

ناﺰ

PGC-1α

) ﯽﺘﺑﺎﯾد لﺮﺘﻨﮐ ﻪﺑ ﺖﺒﺴﻧ ﻢﻟﺎﺳ لﺮﺘﻨﮐ هوﺮﮔ رد 017

/0 (P=

ﻪﺑ رﻮﻃ ﻨﻌﻣ ﺎ يراد .دﻮﺑ ﺮﺘﺸﯿﺑ ﺑﻪ

ﺰﺟ نژ نﺎﯿﺑ ﻦﯿﻣرﻮﻔﺘﻣ هوﺮﮔ

PGC1-α

هوﺮﮔ رد ﺎﻫ لﺮﺘﻨﮐ هوﺮﮔ ﻪﺑ ﺖﺒﺴﻧ يرادﺎﻨﻌﻣ ﺶﯾاﺰﻓا ﺮﮕﯾد ﯽﻧﺎﻣرد ي

) ﺖﺷاد ﯽﺘﺑﺎﯾد 005

/0 نژ نﺎﯿﺑ ﻦﯿﻨﭽﻤﻫ .(P=

PGC-1α

ﺮﮕﯾد ﻪﺑ ﺖﺒﺴﻧ ار رادﺎﻨﻌﻣ ﺶﯾاﺰﻓا و نﺎﯿﺑ ﻦﯾﺮﺘﺸﯿﺑ ﻦﯾﺮﻤﺗ هوﺮﮔ رد هوﺮﮔ

ﺎﻫ ﺖﺷاد

) 001 /0 ﻞﮑﺷ) (P=

1 .(

ﻒﻟا

β