Effects of Aerobic Training on Markers of Renal Oxidative Stress and Serum NOX4 Level in Rats with Myocardial Infarction Model
Shiva Ghadirzad Bashman1 , Roghayeh Pouzesh Jadidi 2 , Jabbar Bashiri 3 , Karim Azali Alamdari4
1. Department of Physical Education, Tabriz Branch, Islamic Azad University, Tabriz, Iran. E-mail: [email protected] 2. Corresponding Author, Department of Physical Education, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
E-mail: [email protected]
3. Department of Physical Education, Tabriz Branch, Islamic Azad University, Tabriz, Iran. E-mail: [email protected]
4. Department of Sport Sciences, Faculty of Psychology and Educational Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran. Email: [email protected]
Article Info ABSTRACT
Article type:
Research
Article history:
Received:
2 November 2021 Received in revised form:
5 February 2022 Accepted:
8 February 2022 Published online:
21 November 2022
Keywords:
aerobic training, Cardio-Renal syndrome, kidney,
oxidative stress
Introduction: Oxidative stress-induced renal injuries are intensified following myocardial infarction (MI) which are associated with a change in Nox4 expression rate. On the other hand, physical training has beneficial effects on renal structure and function. In this study, the effects of aerobic training on serum Nox4 and renal oxidative stress markers were investigated following MI.
Methods: Twenty-four male Wistar rats were randomly divided into three groups (n=8) of Healthy Control, MI Control, and MI Training. Eight weeks of treadmill running training (with an inclination of 0°) at moderate intensity (initiated with 15 min/session running speed at 10 m/min intensity in the first week and progressed to 25 min/session running speed at 30 m/min intensity in the last week) was conducted following MI induction by injections of Isoproterenol (100 mg/kg.day) on two consecutive days. Nox4 Serum level was measured using the flow cytometry method, while renal MDA, carbonylated protein (CP), and 8-OHdG levels were measured via spectrophotometry and colorimetry. The data were analyzed by one- way ANOVA.
Results: While MI induction remarkably increased serum Nox4 as well as renal MDA, CP, and 8-OHdG levels compared to the Healthy Control group, physical training in the MI Training group significantly decreased the amount of these indices (except for CP, P=0.056) compared to Healthy Control group (p=0.001).
However, all indices including serum Nox4 (p=0.029) and renal MDA (p=0.001), CP (p=0.001), and 8-OHdG (p=0.019) levels in the MI Training group, despite a dramatic improvement following physical training were still higher than Healthy Control group.
Conclusion: MI can increase serum Nox4 levels and cause renal oxidative stress.
However, aerobic training could not fully ameliorate these hazardous effects of MI, despite the significant reversal of this trend. It seems that there is a need to identify the best effective training protocols in this field in addition to eliminating the limitations of this study.
Cite this article: Ghadirzad B. S., Pouzesh Jadidi R., Bashiri J., Azali Alamdari K. (2022). Effects of Aerobic Training on Markers of Renal Oxidative Stress and Serum NOX4 Level in Rat Model of Myocardial Infraction. Journal of Sport Biosciences. 2022; 14 (3): 15-29.DOI: https://doi.org/10.22059/jsb.2022.333340.1495
© The Author(s). Publisher: University of Tehran, Faculty of Sport Sciences and Health.
ExtendedAbstract
Introduction
Cardiovascular diseases and acute or chronic renal damage cause mutual risk for each other (1) and in the case of heart or kidney disorder, the interaction of the two organs worsens the existing conditions (2). Also, following a myocardial infarction (MI), a decrease in cardiac output causes a decrease in the function of peripheral organs, including the kidney (3), and as a result, acute and chronic renal damage increases (4). In addition, MI patients are generally transferred too quickly to the catheterization unit and injected with contrast media, which worsens the renal complications of MI due to the rapid toxic effects of these agents on the kidneys (5). Therefore, the occurrence of renal damage in MI patients is quite expected and common (6).In kidney disease (KD), several factors are released into the bloodstream that can induce a series of changes in the heart, leading to a wide variety of clinical situations called cardiorenal syndrome (CRS). Reactive oxygen species (ROS) play an important role in the signaling and progression of systemic inflammatory conditions, as observed in KD(7).Oxidative stress-induced renal injuries are intensified following MI which are associated with a change in NADPH oxidase 4 (Nox4) expression rate. Nox4 is expressed at high levels in kidney tubular cells, and at lower levels in endothelial cells, cardiomyocytes, and other cell types under physiological conditions (14). On the other hand, physical training has beneficial effects on renal structure and function. But so far, the effect of aerobic training on the amount of circulating Nox4 and other indices related to renal oxidative damage has not been investigated directly in rats with an MI-induced model.
Methods
Twenty-four male Wistar rats were randomly divided into three groups (n=8) of Healthy Control, MI Control, and MI Training. Eight weeks of treadmill running training (with an inclination of 0°) at moderate intensity (initiated with 15 min/session running speed at 10 m/min intensity in the first week and progressed to 25 min/session running speed at 30 m/min intensity in the last week) was conducted following MI induction by injections of Isoproterenol (100 mg/kg.day) on two consecutive days. Nox4 Serum level was measured using the flow cytometry method, while renal MDA, carbonylated protein (CP), and 8-OHdG levels were measured via spectrophotometry and colorimetry. The data were analyzed by one-way ANOVA.
Results
While MI induction significantly increased serum Nox4 as well as renal MDA, CP, and 8-OHdG levels compared to the Healthy Control group, physical training in the MI Training
group significantly decreased the amount of these indices (except for CP, P=0.056) compared to Healthy Control group (p=0.001). However, all indices including serum Nox4 (p=0.029) and renal MDA (p=0.001), CP (p=0.001), and 8- OHdG (p=0.019) levels in the MI Training group, despite a dramatic improvement following physical training were still higher than Healthy Control group.
Conclusion
MI can increase serum Nox4 levels and cause renal oxidative stress. However, aerobic training could not fully ameliorate these hazardous effects of MI, despite the significant reversal of this trend. It seems that there is a need to identify the best effective training protocols in this field in addition to eliminating the limitations of this study.
Acknowledgments: Weappreciate the cooperation of dear professors and all those who helped us in this research.
Ethical Considerations
Compliance with ethical guidelines: The present study was conducted following ethical principles.
Funding: The research was done without using financial resources.
Authors’ contribution: Every author contributes to the research equally.
Conflict of interest: No conflict of interest is declared.
Acknowledgments: Thanks to all people help us to conduct the research.
تاﺮﯿﺛﺄﺗ ﺮﻤﺗ
ﯾ ﻦ زاﻮﻫ ي ﺺﺧﺎﺷ ﺮﺑ ﺎﻫ
ي ﺎﺴﮐا سﺮﺘﺳا ﯾ
ﺸ ﯽ ﻠﮐ ﯿ ﻪ راﺪﻘﻣ و مﺮﺳ
NOX4شﻮﻣ ﺎﻫ ي اﺮﺤﺻ ﯽﯾ
لﺪﻣ ﯽﺒﻠﻗ ﮥﺘﮑﺳ
ﻦﻤﺸﺑ دازﺮﯾﺪﻗ اﻮﯿﺷ يﺪﯾﺪﺟ شزﻮﭘ ﻪﯿﻗر ، 1
2
،
يﺮﯿﺸﺑ رﺎﺒﺟ يراﺪﻤﻠﻋ ﯽﻟازآ ﻢﯾﺮﮐ ، 3
4
1 ﮔ.
ﺑﺮﺗ هوﺮ ﯿ ﺖ ﻧﺪﺑ ﯽ
، ﺮﺒﺗ ﺪﺣاو ﯾ
،ﺰ ﻣﻼﺳا دازآ هﺎﮕﺸﻧاد ﯽ
، ﺮﺒﺗ ﯾ
،ﺰ اﯾ ناﺮ :ﻪﻣﺎﻧﺎﯾار .
@gmail.com 2
Shiva.ghadirzad
2 . ﺪﻨﺴﯾﻮﻧ ة ،لوﺆﺴﻣ هوﺮﮔ ﯽﻧﺪﺑ ﺖﯿﺑﺮﺗ
، ﺪﺣاو ﺰﯾﺮﺒﺗ
، ﮕﺸﻧاد ﻣﻼﺳا دازآ هﺎ ﯽ
، ﺰﯾﺮﺒﺗ
، ا ﯾ اﺮ :ﻪﻣﺎﻧﺎﯾار .ن [email protected]
3 . هوﺮﮔ ﯽﻧﺪﺑ ﺖﯿﺑﺮﺗ
، ﺪﺣاو ﺰﯾﺮﺒﺗ
، ﻣﻼﺳا دازآ هﺎﮕﺸﻧاد ﯽ
، ﺰﯾﺮﺒﺗ
، ا ﯾ اﺮ :ﻪﻣﺎﻧﺎﯾار .ن [email protected]
4 . ﺷزرو مﻮﻠﻋ هوﺮﮔ ﯽ
، ﺪﮑﺸﻧاد ة ﺑﺮﺗ مﻮﻠﻋ ﯿﺘ ﯽ ناور و ﺳﺎﻨﺷ ﯽ
، ﻬﺷ هﺎﮕﺸﻧاد ﯿﺪ ﻧﺪﻣ ﯽ ﺎﺑرذآ ﯾ
،نﺎﺠ ﺮﺒﺗ ﯾ
،ﺰ اﯾ ناﺮ . :ﻪﻣﺎﻧﺎﯾار zaruniv.ac.ir k.azali@a
ﻪﻟﺎﻘﻣ تﺎﻋﻼﻃا هﺪﯿﮑﭼ
:ﻪﻟﺎﻘﻣ عﻮﻧ ﯽﺸﻫوﮋﭘ
رﺎﺗ ﯾ ﺦ رد ﯾ ﺖﻓﺎ : 11 / 08 / 1400
:يﺮﮕﻧزﺎﺑ ﺦﯾرﺎﺗ 16
/ 11 / 1400
رﺎﺗ ﯾ ﺦ ﺬﭘ ﯾ شﺮ : 19 / 11 / 1400
رﺎﺗ ﯾ ﺦ رﺎﺸﺘﻧا : 30 / 08 / 1401
ﺪﯿﻠﮐ هژاو :ﺎﻫ
،ﯽﺸﯾﺎﺴﮐا سﺮﺘﺳا
،يزاﻮﻫ ﻦﯾﺮﻤﺗ مورﺪﻨﺳ ﯽﺒﻠﻗ - ﻠﮐ
،يﻮﯿ
ﻪﯿﻠﮐ ﻪﻣﺪﻘﻣ
: ﯽﭘ رد ﯽﺒﻠﻗ ﮥﺘﮑﺳ )
(MI ﺳآ ﯿ ﺐ ﺎﻫ ي ﯽﻣ تﺪﺷ ﯽﺸﯾﺎﺴﮐا سﺮﺘﺳا زا ﯽﺷﺎﻧ يﻮﯿﻠﮐ نﺎﯿﺑ ﺮﯿﯿﻐﺗ ﺎﺑ ﻪﮐ ﺪﻧﺮﯿﮔ
ﻮﺳ زا .ﺖﺳا هاﺮﻤﻫNOX4 ﯾ
،ﯽ ﺮﻤﺗ ﯾ ﻦ ﻧﺪﺑ ﯽ تاﺮﯿﺛﺄﺗ .دراد ﻪﯿﻠﮐ دﺮﮑﻠﻤﻋ و رﺎﺘﺧﺎﺳ ﺮﺑ ﯽﺘﺒﺜﻣ د
ﻦﯾا ر ﺗ ﻖﯿﻘﺤ ﺛﺄﺗ ﯿﺮ ﻦﯾﺮﻤﺗ
راﺪﻘﻣ ﺮﺑ يزاﻮﻫ ﺺﺧﺎﺷ و مﺮﺳNOX4
ﯿﻠﮐ ﯽﺸﯾﺎﺴﮐا ﺐﯿﺳآ يﺎﻫ ﺐﻗﺎﻌﺘﻣ ﻪ
.ﺪﺷ ﯽﺳرﺮﺑMI
:ﺶﻫوﮋﭘ شور 24
اﺮﺤﺻ شﻮﻣ ﯽﯾ
ﺮﻧ ﻪﺑ ﻓدﺎﺼﺗ رﻮﻃ ﯽ هوﺮﮔ ﻪﺳ ﻪﺑ )
8
= ﻞﻣﺎﺷ (n ﺘﻨﮐ ﻢﻟﺎﺳ لﺮ ، لﺮﺘﻨﮐ ﻪﺘﮑﺳ و
ﻪﺘﮑﺳ
ﺮﻤﺗ ﯾ ﻦ ﺴﻘﺗ ﯿ ﻢ ﺪﻧﺪﺷ زا ﺲﭘ . يﺎﻘﻟا ﯽﺒﻠﻗ ﮥﺘﮑﺳ رﺰﺗ ﻂﺳﻮﺗ
ﯾ ﻖ اﯾ لﻮﻧﺮﺗوﺮﭘوﺰ )
100 mg/kg.day د (
ﻟاﻮﺘﻣ زور ود ر ﯽ
، 8
ﺮﻤﺗ ﻪﺘﻔﻫ ﯾ ﻦ ود ﯾ نﺪ ﺷ ﺎﺑ) ﯿ ﺐ ور ﻂﺳﻮﺘﻣ تﺪﺷ ﺎﺑ (ﺮﻔﺻ ي
راﻮﻧ نادﺮﮔ مﺎﺠﻧا ﺖﻓﺮﮔ زا ﻪﮐ 10 ﺮﺘﻣ ﻗد رد ﯿ ﻪﻘ تﺪﻣ ﺎﺑ 15
ﻗد ﯿ ﻪﻘ رد ﺘﻔﻫ ﮥ ﻪﺑ ،لوا 25 ﻗد رد ﺮﺘﻣ ﯿ ﻪﻘ تﺪﻣ و 30 ﻗد ﯿ ﻪﻘ ﺘﻔﻫ رد ﮥ ﺳر ﺮﺧآ ﯿﺪ راﺪﻘﻣ . شور ﻪﺑ مﺮﺳ NOX4
ﺳﻮﻠﻓ ﯿ ﺮﺘﻣﻮﺘ ي
، راﺪﻘﻣ ﺌﺗوﺮﭘ ،MDA
ﯿ ﻦ ﻧﻮﺑﺮﮐ ﯿ ﻪﻠ ) و (CP 8 ﻠﮐOHdG ﯿﻪ ﻃ شور ﻪﺑ ﯿ ﻒ ﺠﻨﺳ ﯽ ﮓﻧر و ﺠﻨﺳ ﯽ مﺎﺠﻧا
ﺖﻓﺮﮔ هداد و ﺎﺑ ﺎﻫ ﻠﺤﺗ ﯿ ﻞ راو ﯾ ﺲﻧﺎ ﻪﻫاﺮﮑﺗ ﻠﺤﺗ ﯿ ﻞ ﺷ ﺪﻧﺪ .
ﯾ ﻪﺘﻓﺎ ﺎﻫ : ﻟﺎﺣرد ﯽ ﻪﮐ يﺎﻘﻟا ﺶﯾاﺰﻓا ﺐﺒﺳMI
رادﺎﻨﻌﻣ راﺪﻘﻣ مﺮﺳNOX4
و ﻨﭽﻤﻫ ﯿ ﻦ
، MDA وCP
8OHdG
ﻠﮐ ﯿﻪ ﻪﺑ ﺖﺒﺴﻧ هوﺮﮔ لﺮﺘﻨﮐ ﻢﻟﺎﺳ هوﺮﮔ رد ﻦﯾﺮﻤﺗ مﺎﺠﻧا ﺎﻣا ،ﺪﺷ
ﺘﮑﺳ ﻪ ﺮﻤﺗ ﯾ ﻦ نﺪﺷ ﺮﺘﻤﮐ ﺐﺒﺳ ﯾا راﺪﻘﻣ
ﺺﺧﺎﺷ ﻦ ﺎﻫ ﻪﺑ ﺰﺟ
)CP 056 /0 ﻪﺑ ﺖﺒﺴﻧ (P=
هوﺮﮔ ﻪﺘﮑﺳ لﺮﺘﻨﮐ ﺪﺷ ) 001 /0 ( .P=
ﺎﺑ اﯾ ﻦ لﺎﺣ
، راﺪﻘﻣ ﺮﻫ رﺎﻬﭼ ﺺﺧﺎﺷ ﻞﻣﺎﺷ NOX4
مﺮﺳ ) 029 /0 (P=
ﻦﯿﻨﭽﻤﻫ و )MDA
001 /0 (،P=
)CP 001 /0 ( P=
و 8OHdG )
019 /0 (P=
ﻠﮐ ﯿﻪ رد هوﺮﮔ
ﺮﻤﺗ ﻪﺘﮑﺳ ﯾ ﻦ
،(ﺮﯿﮕﻤﺸﭼ دﻮﺒﻬﺑ دﻮﺟو ﺎﺑ) زﻮﻨﻫ
ﻢﻫ زا ﺮﺘﺸﯿﺑ هوﺮﮔ لﺮﺘﻨﮐ ﻢﻟﺎﺳ دﻮﺑ
.
ﺘﻧ ﯿ ﻪﺠ ﮔﯿ ﺮ ي : ﯽﻣMI ﺶﯾاﺰﻓا ﺐﺒﺳ ﺪﻧاﻮﺗ ﯾﺎﺴﮐا سﺮﺘﺳا زوﺮﺑ و مﺮﺳNOX4
ﺎﺑ .دﻮﺷ ﻪﯿﻠﮐ رد ﯽﺸ ﻦﯾا
لﺎﺣ ﻦﯾﺮﻤﺗ ،
ﻈﺣﻼﻣ ﻞﺑﺎﻗ ندﺮﮐ سﻮﮑﻌﻣ دﻮﺟو ﺎﺑ يزاﻮﻫ ﮥ
ﻦﯾا ﻞﻣﺎﮐ ﻊﻓر ﻪﺑ ردﺎﻗ ،ﺪﻧور ﻦﯾا تاﺮﯿﺛﺄﺗ
ﺒﻧMI ﻪﺑ .دﻮ ﯽﻣ ﺮﻈﻧ رد ﻪﮐ ﺪﺳر
هﺪﻨﯾآ تﺎﻘﯿﻘﺤﺗ هوﻼﻋ
ﺖﯾدوﺪﺤﻣ ﻊﻓر ﺮﺑ ﻦﯾﺮﺘﻬﺑ ﯽﯾﺎﺳﺎﻨﺷ ﻪﺑ زﺎﯿﻧ ،ﺮﺿﺎﺣ ﻖﯿﻘﺤﺗ يﺎﻫ
ﻞﮑﺗوﺮﭘ ﺎﻫ ي ﯽﻨﯾﺮﻤﺗ ﺆﻣ ﺮﺛ د ﻦﯾا ر
.ﺖﺳا ﯽﻗﺎﺑ ﻪﻨﯿﻣز
دﺎﻨﺘﺳا : ﺪﻗ ﯾ دازﺮ ﻦﻤﺸﺑ ، اﻮﯿﺷ
؛ يﺪﯾﺪﺟ شزﻮﭘ ،
ﻪﯿﻗر ؛ يﺮﯿﺸﺑ ، ﻢﯾﺮﮐ ؛يراﺪﻤﻠﻋ ﯽﻟازآ و رﺎﺒﺟ .
ﺮﻤﺗ تاﺮﯿﺛﺄﺗ ﯾ ﻦ زاﻮﻫ ي ﺺﺧﺎﺷ ﺮﺑ ﺎﻫ ي ﺎﺴﮐا سﺮﺘﺳا ﯾ
ﺸ ﯽ ﻠﮐ ﯿﻪ ﻣ و راﺪﻘ NOX4
مﺮﺳ شﻮﻣ ﺎﻫ ي اﺮﺤﺻ ﯽﯾ ﯽﺒﻠﻗ ﮥﺘﮑﺳ لﺪﻣ .
ﺮﺸﻧ ﯾﮥ ﯽﺘﺴﯾز مﻮﻠﻋ ﺷزرو
ﯽ . 1401
؛ ) 3(
14 : 15-29
. DOI: https://doi.org/10.22059/jsb.2022.333340.1495
ﺎﻧ ،ناﺮﻬﺗ هﺎﮕﺸﻧاد :ﺮﺷ ﺪﮑﺸﻧاد
ة ﯽﺷزرو مﻮﻠﻋ و
ﯽﺘﺳرﺪﻨﺗ . ﺴﯾﻮﻧ©
.نﺎﮔﺪﻨ
18
ﻋ ﺰﯿﯾﺎﭘ ،مﻮﺳ ةرﺎﻤﺷ ،ﻢﻫدرﺎﻬﭼ ةرود ،ﯽﺷزرو ﯽﺘﺴﯾز مﻮﻠ 1401
ﻪﻣﺪﻘﻣ يرﺎﻤﯿﺑ يﺎﻫ ﺒﻠﻗ ﯽ - ﻗوﺮﻋ ﯽ و ﺳآ ﯿ ﺐ ﺎﻫ ي ﺪﻧﺮﮕﯾﺪﮑﯾ ياﺮﺑ ﻞﺑﺎﻘﺘﻣ ﺮﻄﺧ دﺎﺠﯾا ﻞﻣﺎﻋ ﻪﯿﻠﮐ ﻦﻣﺰﻣ ﺎﯾ دﺎﺣ )1
( و رد نﺎﻣز زوﺮﺑ لﻼﺘﺧا ﺒﻠﻗ ﯽ ﯾﺎ
ﻠﮐ ﯿﻮ ي
، ﺶﻨﮑﻤﻫﺮﺑ ﺪﯾﺪﺸﺗ ﺐﺒﺳ ماﺪﻧا ود
اﺮﺷ ﯾ ﻂ دﻮﺟﻮﻣ ﻣ ﯽ ﻮﺷ د )2 ( ﻦﯿﻨﭽﻤﻫ . ﯽﭘ رد
ﯽﺒﻠﻗ ﮥﺘﮑﺳ
1 ) ﺐﺒﺳ ﺐﻠﻗ هﺪﻧوﺮﺑ ﺶﻫﺎﮐ ،ﻢﻫ (MI
ﺶﻫﺎﮐ ماﺪﻧا دﺮﮑﻠﻤﻋ يﺎﻫ
ﭘﯿ ﻧﻮﻣاﺮ ﯽ ﻪﻠﻤﺟ زا ﻠﮐ ﯿﻪ ﯽﻣ دﻮﺷ ) 3(
ﻦﯾاﺮﺑﺎﻨﺑ و ﺳآ
ﯿ ﺐ ﺎﻫ ي ﯽﻣ تﺪﺷ ﻪﯿﻠﮐ ﻦﻣﺰﻣ و دﺎﺣ ﺪﻧﺮﯿﮔ
)4 ( . نوﺰﻓا ﻦﯾا ﺮﺑ
ًﺎﻣﻮﻤﻋ نارﺎﻤﯿﺑ نﻮﯿﺳاﺰﯾﺮﺗﺎﮐ ﺶﺨﺑ ﻪﺑ ﻊﯾﺮﺳ ﯽﻠﯿﺧ MI
ﻖﯾرﺰﺗ ﺖﺤﺗ و هﺪﺷ هداد لﺎﻘﺘﻧا2
دﺎﻣ ة ﺐﺟﺎﺣ ﯽﻣ راﺮﻗ3
ﻪﮐ ﺪﻧﺮﯿﮔ ﻪﺑ
ندﻮﺑ اراد ﻞﯿﻟد
تاﺮﯿﺛﺄﺗ ﻪﯿﻠﮐ ﺮﺑ داﻮﻣ ﻦﯾا ﻊﯾﺮﺳ ﯽﻠﯿﺧ ﯽﻤﺳ ﺎﻫ
)5 ( زا ﯽﺷﺎﻧ ضراﻮﻋ ، ﯽﺒﻠﻗ ﮥﺘﮑﺳ
ﻪﯿﻠﮐ ﺮﺑ ﻢﯿﺧو ﺎﻫ ﯽﻣ ﻢﻫ ﺮﺗ زوﺮﺑ ﻦﯾاﺮﺑﺎﻨﺑ .دﻮﺷ
ﺳآ ﯿ ﺐ ﺎﻫ ي
نارﺎﻤﯿﺑ رد يﻮﯿﻠﮐ
ًﻼﻣﺎﮐMI
رﻮﻣ ﺖﺳا لواﺪﺘﻣ و رﺎﻈﺘﻧا د )6
( ﯿﺳآ رد ﯽﻟو . ﺐ
و ﺎﻫ ﺑﯿ رﺎﻤ ي ﺎﻫ ي ﻪﻠﻤﺟ زا ﻞﻣﺎﻋ ﻦﯾﺪﻨﭼ يﻮﯿﻠﮐ ﻪﻧﻮﮔ
ﺎﻫ ي لﺎﻌﻓ
ﯽﻣ نﮋﯿﺴﮐا يﺮﺳ ﮏﯾ دﺎﺠﯾا ﺐﺒﺳ ﺪﻨﻧاﻮﺗ
ﻌﻗﻮﻣ ﯿ ﺖ ﺎﻫ ي ﻪﺑ مﻮﺳﻮﻣ ﯽﻨﯿﻟﺎﺑ عﻮﻨﺘﻣ مورﺪﻨﺳ
ﯽﺒﻠﻗ - يﻮﯿﻠﮐ )4
.ﺪﻧﻮﺷ (CRS
لﺎﺜﻣ ياﺮﺑ رد
مورﺪﻨﺳ
ﻢﯾﺰﻧآ ﻂﺳﻮﺗ لﺎﮑﯾدار نﮋﯿﺴﮐا ،CRF NADPH
) زاﺪﯿﺴﮐا هﺰﯿﻟﺎﺗﺎﮐ (NOX
ﯽﻣ دﻮﺷ ﯽﻣ و ﺎﺑ ﺪﻧاﻮﺗ ﺪﯿﺴﮐا ﮏﯾﺮﺘﯿﻧ )5
ﻞﯿﮑﺸﺗ ياﺮﺑ (NO
ﺖﯾﺮﺘﯿﻧ ﯽﺴﮐاﺮﭘ ﯽﻤﮑﺴﯾا ﺐﯿﺳآ زا ﻪﺒﻨﺟ ﻦﯾﺪﻨﭼ لﻮﺌﺴﻣ ﻪﮐ ﺪﻫد ﺶﻨﮐاو6
- يﻮﯿﻠﮐ دﺪﺠﻣ ﻖﯾرﺰﺗ ﺖﺳا
) 7(
.
ﺰﯿﻧ ﻪﯿﻠﮐ رد ،
NADPH
) ﺎﻫزاﺪﯿﺴﮐا
NOXNoxs
ﺪﻨﺘﺴﻫ ﻮﯿﺗاﺪﯿﺴﮐا سﺮﺘﺳا ﯽﻠﺻا ﻊﺑﺎﻨﻣ ( )8
( مﺎﯿﭘ رد ﺎﻣا ، ﺰﯾﺎﻤﺗ ،زﻮﺘﭘﻮﭘآ ،نژ نﺎﯿﺑ ﯽﻧﺎﺳر
ﺮﯿﺜﮑﺗ و لﻮﻠﺳ ﺎﻫ ي ﺪﻧراد ﺖﯿﻤﻫا رﺎﯿﺴﺑ ﺰﯿﻧ ﻪﯿﻠﮐ )9
( توﺎﻔﺘﻣ مﺮﻓوﺰﯾا ﻪﺳ ﻞﻗاﺪﺣ . ﻞﻣﺎﺷNOX
NOX4
، وNOX2
نﺎﯿﺑ ﻪﯿﻠﮐ ردNOX1
ﯽﻣ دﻮﺷ ) 10 ( ﭻﯿﻫ ﺪﻨﭼﺮﻫ . ﺴﯾﺎﻘﻣ
ﮥ مﺎﺠﻧا ﯽﻘﯿﻗد ﻧ
ﺘﻓﺮﮕ ﻪ ﺎﻣا ،ﺖﺳا ﻪﺑ ﯽﻣ ﺮﻈﻧ ﺪﺳر ﻦﯿﻤﻫ ﻪﺑ و ﺖﺳا ﻪﯿﻠﮐ رد دﻮﺟﻮﻣ مﺮﻓوﺰﯾا ﻦﯾﺮﺘﺸﯿﺑNOX4
) يﻮﯿﻠﮐ زاﺪﯿﺴﮐا مﺎﻧ ﻪﺑ اﺪﺘﺑا ﻞﯿﻟد
RENOX
( ﯽﻣ ﻪﺘﺧﺎﻨﺷ7
ﺪﺷ ) 10 ( .
4
ﺐﻠﻏاNOX
لﻮﻠﺳ رد دراد راﺮﻗ ﻪﯿﻠﮐ ﯽﻟﻮﺑﻮﺗ يﺎﻫ )
10 ( رد ﺎﻣا ، ﺮﯾﺎﺳ
لﻮﻠﺳ ﻪﻠﻤﺟ زا ﺎﻫ لﻮﻠﺳ
ﺎﻫ ي ﯽﻟوﺮﻣﻮﻠﮔ لﺎﯾﮋﻧاﺰﻣ ﯽﻣ ﺖﻓﺎﯾ ﺮﺘﻤﮐ راﺪﻘﻣ ﻪﺑ ﺰﯿﻧ8
دﻮﺷ ) 11 ( نﺎﯿﺑ ﻦﯾﺮﺘﺸﯿﺑ ﮏﯾژﻮﻟﻮﯾﺰﯿﻓ ﻂﯾاﺮﺷ رد .
4 NOX
رد نﺎﮔﺪﻧﻮﺟ لﻮﻠﺳ
ﺎﻫ ي ﯽﻣ قﺎﻔﺗا (ﻪﻨﺗ ﻪﺑ ﮏﯾدﺰﻧ) لﺎﻤﯾﺰﮔوﺮﭘ ﯽﻟﻮﺑﻮﺗ ﺪﺘﻓا
) 10 ( ﺎﺑ . ﻦﯾا لﺎﺣ دﺮﮑﻠﻤﻋ ﯽﮑﯿﺘﻧژ فﺬﺣ ﭗﯿﺗﻮﻨﻓ ﻪﺑNOX4
ﺖﺨﯾر رد يزرﺎﺑ ﯽﻤﻧ ﺮﺠﻨﻣ شﻮﻣ ﯽﻟﻮﺑﻮﺗ و ﯽﻗوﺮﻋ ﯽﺳﺎﻨﺷ
ﻓ و دﻮﺷ يﻮﯿﻠﮐ نﻮﯿﺳاﺮﺘﻠﯿ ﯽﻣ ﺖﺑﺎﺛ۹
ﺪﻧﺎﻣ ) 12 ( و
ًﻻﻮﺻا نﻮﻨﮐﺎﺗ ﭻﯿﻫ ﺶﻬﺟ ﻪﻧﻮﮔ
ﯽﮑﯿﺘﻧژ ﺖﺳا هﺪﺸﻧ هﺪﻫﺎﺸﻣ يرﺎﻤﯿﺑ ﻪﺑ هﺪﻧﻮﺷﺮﺠﻨﻣ NOX4
) 13 ( ﺖﯿﻌﺿو ﻦﯾﺪﻨﭼ ﺎﻣا . ﺪﻨﻧﺎﻣ
ﻪﺑ ﯽﺒﻠﻗ هﺪﻧوﺮﺑ ﺶﻫﺎﮐ ﺮﺛا رد) ﯽﺴﮐﻮﭙﯿﻫ
ﻪﯿﻠﮐ ﺐﻗﺎﻌﺘﻣ ﺎﻫ بﺎﻬﺘﻟا ،(MI
رد ﻪﮐ) ﻪﯿﻠﮐ ردMI
ﯽﻣ هﺪﻫﺎﺸﻣ ﻢﻫ ﺎﻫ ﮏﯿﺗوﺮﺒﯿﻓوﺮﭘ ﻞﻣاﻮﻋ و (دﻮﺷ
ﺪﻨﻧﺎﻣ۱۰
β۱۱
ﻪﺑ ،TGF
ا نﺎﯿﺑ راﺪﻘﻣ ﺶﯾاﺰﻓ
ﺮﺠﻨﻣ NOX4
ﯽﻣ ﺪﻧﻮﺷ ) 14 ( ﺖﯿﻟﺎﻌﻓ ﺎﯾ نﺎﯿﺑ ﺶﯾاﺰﻓا تﺎﻘﯿﻘﺤﺗ ﯽﺧﺮﺑ رد ﻦﯿﻨﭽﻤﻫ . ﻪﺘﺴﻧاد ﻂﺒﺗﺮﻣ ﮏﯾژﻮﻟﻮﺗﺎﭘ ﺖﯿﻌﺿو ﻦﯾﺪﻨﭼ ﻪﺑNOX4
ﺎﻨﺒﻣ ﺮﺑ و ﺖﺳا هﺪﺷ ي
ﯾﺮﻈﻧ ﮥ ﯽﻣ رﻮﺼﺗ ﯽﺸﯾﺎﺴﮐا سﺮﺘﺳا ﺶﯾاﺰﻓا ﻪﮐ دﻮﺷ
يرﺎﻤﯿﺑ ﯽﺧﺮﺑ ﺖﻓﺮﺸﯿﭘ و ﯽﻟﻮﻠﺳ ﺐﯿﺳآ ﺶﯾاﺰﻓا ﺐﺒﺳNOX4
ﺎﻫ
ﯽﻣ ﺐﯿﺳآ تﺪﺷ و دﻮﺷ دراد ﯽﮑﯾدﺰﻧ و ﻢﯿﻘﺘﺴﻣ طﺎﺒﺗرا بﺎﻬﺘﻟا و ﯽﺸﯾﺎﺴﮐا سﺮﺘﺳا ﺎﺑ ﻪﯿﻠﮐ ﺖﻓﺎﺑ ﻪﺑ درﺎﮐﻮﯿﻣ سﻮﺘﮐرﺎﻔﻧا ﻂﺳﻮﺗ هدراو يﺎﻫ
) 15 ( ﻘﯿﻘﺤﺗ رد . ﯽ
شﻮﻣ رد لﺪﻣ يﺎﻫ ﺘﮑﺳ
ﮥ ﺪﻗﺎﻓ ﯽﮑﯿﺘﻧژ ﺮﻈﻧ زا ﻪﮐ دﺎﺣ ﯽﺒﻠﻗ ﺪﺷ يﺮﯿﮔﻮﻠﺟ ﮏﯿﻤﮑﺴﯾا تﺎﻌﯾﺎﺿ زا ،ﺪﻧدﻮﺑNOX4
) 16 ( ،
ﻟﺎﺣرد ﯽ ﻪﮐ ،نﺎﺴﮑﯾ ﻞﮑﺗوﺮﭘ ﺎﺑ ﺮﮕﯾد ﯽﻘﯿﻘﺤﺗ رد ﺛﺄﺗ
ﯿﺮ ي ﻣ ﺪﺸﻧ هﺪﻫﺎﺸ )
17 (.
ﯽﻤﮑﺴﯾا ﺐﯿﺳآ ﺐﻗﺎﻌﺘﻣ ﻂﯾاﺮﺷ رد ،ﻦﻤﺿ رد -
ﯽﺘﻧآ ﻦﯿﺑ نزاﻮﺗ مﺪﻋ ،دﺪﺠﻣ ﻖﯾرﺰﺗ ناﺪﯿﺴﮐا
ناﺪﯿﺴﮐا و ﺎﻫ ﻞﻤﺘﺤﻣ ﻢﻫ ﺎﻫ
ﺖﺳا ) 18 ( زا ﻪﮐ
ﻦﯿﺌﺗوﺮﭘ ﻪﺑ ﺐﯿﺳآ ﻖﯾﺮﻃ ﯽﺑﺮﭼ ،ﺎﻫ
و ﺎﻫ ﺪﺷ ﺪﻫاﻮﺧ ﯽﻟﻮﻠﺳ ﺐﯿﺳآ ﺮﺘﺸﯿﺑ ﺖﻓﺮﺸﯿﭘ ﺐﺒﺳ مﺎﺠﻧاﺮﺳ ،DNA
) 19 ( هزاﺪﻧا ﺎﺑ ﻪﮐ تﻻﻮﺼﺤﻣ يﺮﯿﮔ
1. Myocardial infarction
2. Catheterization
3. contrast media
4. cardiorenal syndrome- Cardiorenal Syndrome
5. Nitric Oxide
6. Peroxynitrite
7. Renal Oxidase (RENOX)
8. Glomerular Mesangial cells
9. Renal filtration
10. Profibrotic
11 . Transforming Growth Factor Beta
ﺮﻤﺗ تاﺮﯿﺛﺄﺗ ﯾ
ﻦ زاﻮﻫ ي ﺺﺧﺎﺷ ﺮﺑ ﺎﻫ ي ﺎﺴﮐا سﺮﺘﺳا ﯾ
ﺸ ﯽ ﻠﮐ ﯿ ﻪ راﺪﻘﻣ و شﻮﻣ مﺮﺳNOX4
ﺎﻫ ي اﺮﺤﺻ ﯽﯾ ﯽﺒﻠﻗ ﮥﺘﮑﺳ لﺪﻣ
19
ﻦﯿﺌﺗوﺮﭘ زا ﻖﺘﺸﻣ ﻒﻠﺘﺨﻣ و ﺎﻫﺪﯿﭙﯿﻟ ،ﺎﻫ
ﻞﺑﺎﻗDNA
.ﺖﺳا ﯽﺳرﺮﺑ لﺎﺜﻣ ياﺮﺑ
OHdG1
-
ﯾ8
ﮑ ﯽ زا ﺎﻫﺮﮐرﺎﻣ ي سﺎﺴﺣ رﺎﯿﺴﺑ ﯽﺘﺴﯾز ﺖﺳا
ﻮﯿﺗاﺪﯿﺴﮐا ﺐﯿﺳآ ﻦﯿﯾﺎﭘ رﺎﯿﺴﺑ ﺢﻄﺳ سﺎﮑﻌﻧا ﻪﺑ ردﺎﻗ ﯽﺘﺣ ﻪﮐ ﺖﺳاDNA
) 20 ( ﺎﺑ . اﯾ ﻦ راﺪﻘﻣ نﻮﻨﮐﺎﺗ ،لﺎﺣ
8-OHdG
ﺐﻗﺎﻌﺘﻣ ﻪﯿﻠﮐ ﺖﻓﺎﺑ
ﯽﺳرﺮﺑ MI
.ﺖﺳا هﺪﺸﻧ هوﺮﮔ
ﺎﻫ ي ﻪﻠﯿﻧﻮﺑﺮﮐ ﻦﯿﺌﺗوﺮﭘ )2
ﺰﯿﻧ (CP
ﻪﺑ ناﻮﻨﻋ ﻦﯿﺌﺗوﺮﭘ ﻮﯿﺗاﺪﯿﺴﮐا ﺐﯿﺳآ ﺮﮕﻧﺎﺸﻧ ﺶﯾاﺰﻓا ﻪﯿﻠﮐ دﺎﺣ ﺐﯿﺳآ رد ﺎﻫ
ﯽﻣ ﺪﻨﻧﺎﻣ يﺪﯿﭙﯿﻟ نﻮﯿﺳاﺪﯿﺴﮐاﺮﭘ تﻻﻮﺼﺤﻣ ﻪﺑ ﺖﺒﺴﻧ و ﺪﻨﺑﺎﯾ ﺪﻧﺮﺗراﺪﯾﺎﭘ MDA
) 21 ( ﺎﺑ . اﯾ ﻦ ﻞﯿﻧﻮﺑﺮﮐ ﻦﯿﺌﺗوﺮﭘ ﺖﻈﻠﻏ نﻮﻨﮐﺎﺗ ،لﺎﺣ
3
ﻪﺑ ناﻮﻨﻋ زا ﯽﺷﺎﻧ يﻮﯿﻠﮐ ﯽﺸﯾﺎﺴﮐا سﺮﺘﺳا ﯽﺘﺴﯾز ﺮﮕﻧﺎﺸﻧ ﯽﺒﻠﻗ ﮥﺘﮑﺳ
.ﺖﺳا هﺪﺸﻧ ﯽﺑﺎﯾزرا
ﻖﯿﻗد ﺶﻘﻧ ﻢﻫ زﻮﻨﻫ ﻪﮑﻨﯾا ﻦﻤﺿ ﻪﯿﻠﮐ ردNOX4
ًﺎﺘﺒﺴﻧ ﺶﻘﻧ زا ﯽﮐﺎﺣ دﻮﺟﻮﻣ ﺪﻫاﻮﺷ ،ﺖﺳا ﻪﺘﺧﺎﻨﺷﺎﻧ ﻧﺎﮔود
ﮥ ﻪﯿﻠﮐ رد NOX4
و ﺎﻫ
ﺪﻨﺘﺴﻫ ﺐﻠﻗ )
13 ( و ﻪﺑ ﯽﻣ ﺮﻈﻧ راﺪﻘﻣ ﯽﺳرﺮﺑ ﻪﮐ ﺪﺳر مﺮﺳNOX4
نارﺎﻤﯿﺑ رد زا عﻼﻃا ﺐﺴﮐ ﺎﺑ نﺎﻣﺰﻤﻫMI
ﺺﺧﺎﺷ ﺎﻫ ي ﺐﯿﺳآ ﻪﺑ طﻮﺑﺮﻣ
.ﺪﺷﺎﺑ ﻪﺘﺷاد تﺎﻣﺎﻬﺑا ﻦﯾا ﻊﻓر رد يدﺎﯾز رﺎﯿﺴﺑ ﺖﯿﻤﻫا ﻪﯿﻠﮐ ﺖﻓﺎﺑ ﯽﺸﯾﺎﺴﮐا ﻮﺳ زا ﯾ ﯽ ﻟﺎﻌﻓ ﯿ ﺖ ﻧﺪﺑ ﯽ ﯽﻧاﻮﺗزﺎﺑ رد يروﺮﺿ ﻞﻣﺎﻋ ﮏﯾ ﺐﻗﺎﻌﺘﻣ ﯽﺒﻠﻗ
ﯽﺒﻠﻗ ﮥﺘﮑﺳ ﺖﺳا
) 22 ( ﻦﯾا رد و ﻪﻨﯿﻣز ﺛﺄﺗ ﯿﺮ ﺪﯾﺪﺷ ﯽﺑوﺎﻨﺗ تﺎﻨﯾﺮﻤﺗ
يزﺎﺳزﺎﺑ ﺮﺑ دﻮﺗ
ة رﺎﭼد نارﺎﻤﯿﺑ ﭗﭼ ﻦﻄﺑ )MI
23 ( ﺺﺧﺎﺷ ﺶﻫﺎﮐ ، يﺎﻫ
ﯽﺒﻠﻗ ﮥﺘﮑﺳ تﺎﻧاﻮﯿﺣ رد
)MI
24 ( ﺎﯾ و ﺛﺄﺗ ﯿﺮ تﺎﻨﯾﺮﻤﺗ ﻢﮐ ﻢﺠﺣ
نارﺎﻤﯿﺑ ﻦﯾا ﯽﺴﻔﻨﺗ ﯽﺒﻠﻗ ﯽﮔدﺎﻣآ دﻮﺒﻬﺑ ﺮﺑ ،ﺪﯾﺪﺷ )
25 ( ﺄﺗ ﯿﯾ ﺪ زا ﺲﭘ يزاﻮﻫ تﺎﻨﯾﺮﻤﺗ ﻪﮑﻨﯾا ﺎﺑ ﯽﻟو .ﺖﺳا هﺪﺷ ﯽﺒﻠﻗ ﮥﺘﮑﺳ
تاﺮﯿﺛﺄﺗ رﺎﯿﺴﺑ
ﯽﺘﺒﺜﻣ ﻪﯿﻠﮐ يود ﺮﻫ ﺮﺑ )
26 ( ﺐﻠﻗ و ) 22
، 27-29 ( ﻪﺘﺷاد نﻮﻨﮐﺎﺗ ﺎﻣا ،ﺪﻧا ﺛﺄﺗ
ﯿﺮ يزاﻮﻫ تﺎﻨﯾﺮﻤﺗ ﻪﺑ
رﻮﻃ راﺪﻘﻣ ﺮﺑ ﻢﯿﻘﺘﺴﻣ شدﺮﮔNOX4
ﺳ ﺎﺑ هاﺮﻤﻫ نﻮﺧ ﺮﯾﺎ
ﺺﺧﺎﺷ ﺎﻫ ي شﻮﻣ رد يﻮﯿﻠﮐ ﯽﺸﯾﺎﺴﮐا ﺐﯿﺳآ ﻪﺑ طﻮﺑﺮﻣ ﯽﯾﺎﻘﻟا لﺪﻣ يﺎﻫ
درﻮﻣ رد ﺎﻬﻨﺗ .ﺖﺳا هﺪﺸﻧ ﯽﺳرﺮﺑMI
ﺛﺄﺗ ﯿﺮ
يزﺎﺳزﺎﺑ ﺮﺑ يزاﻮﻫ ﻦﯾﺮﻤﺗ دﻮﺗ
ة نﺎﯿﺑ و ﯽﺒﻠﻗ ﺐﻠﻗ رد NOX4
شﻮﻣ ﺎﻫ ي رﺎﭼد ﻒﯿﻔﺧ MI
) 30 ( ، ﺛﺄﺗ ﯿﺮ ﻦﯿﺌﺗوﺮﭘ راﺪﻘﻣ ﺮﺑ ﯽﺑوﺎﻨﺗ ﻦﯾﺮﻤﺗ
و NOX4
حﻮﻄﺳ و MDA
ﻪﻧﻮﮔ ﺎﻫ ي شﻮﻣ ﺐﻠﻗ نﮋﯿﺴﮐا لﺎﻌﻓ رﺎﭼد يﺎﻫ
)MI
31 ( ، ﺛﺄﺗ ﯿﺮ يزﺎﺳزﺎﺑ ﺮﺑ ﯽﺘﻣوﺎﻘﻣ و يزاﻮﻫ ﻦﯾﺮﻤﺗ دﻮﺗ
ة
و ﯽﻨﻄﺑ ﺺﺧﺎﺷ ﺎﻫ ي شﻮﻣ رد ﻮﻠﻗود ﻪﻠﻀﻋ ﯽﺸﯾﺎﺴﮐا سﺮﺘﺳا ﺐﻗﺎﻌﺘﻣ يﺎﻫ
)MI
32 ( و ﺛﺄﺗ ﯿﺮ ﯽﻨﻄﺑ ﯽﻤﺘﯾرآ ﺮﺑ نﺪﯾود ﻦﯾﺮﻤﺗ شﻮﻣ4
يﺎﻫ
رﺎﭼد )MI
33 ( مﺎﺠﻧا ﻢﯿﻘﺘﺴﻣ ﯽﺳرﺮﺑ ﺘﻓﺮﮔ
ﻪ .ﺖﺳا ﻪﺑ ﯽﻣ ﺮﻈﻧ ﯽﺳرﺮﺑ ﻪﺑ زﺎﯿﻧ ﻪﮐ ﺪﺳر ﺛﺄﺗ
ﯿﺮ راﺪﻘﻣ ﺮﺑ يزاﻮﻫ ﻦﯾﺮﻤﺗ ﺎﺑ هاﺮﻤﻫ مﺮﺳNOX4
راﺪﻘﻣ ﺺﺧﺎﺷ ﺎﻫ ي ﻪﯿﻠﮐ ﯽﺸﯾﺎﺴﮐا ﺐﯿﺳآ ﯽﭘ رد
زوﺮﺑ
ًﻼﻣﺎﮐMI
ﯽﻣ رﺎﻈﺘﻧا .ﺪﺷﺎﺑ يروﺮﺿ ،هﮋﯾو ﺖﻋاﺪﺑ ندﻮﺑ اراد ﻦﻤﺿ ، ﻖﯿﻘﺤﺗ ﻦﯾا ﻪﮐ دور
زا ﯽﺒﺳﺎﻨﻣ تﺎﻋﻼﻃا ﺪﻧاﻮﺘﺑ ﺛﺄﺗ
ﯿﺮ زا ﯽﺷﺎﻧ ضراﻮﻋ ﺮﺑ يزاﻮﻫ ﻦﯾﺮﻤﺗ ﻪﯿﻠﮐ ردMI
ﻪﻨﯿﻣز و ﺪﻫد ﺖﺳد ﻪﺑ ﺎﻫ ﻦﯾا رد ﺮﺘﺸﯿﺑ تﺎﻘﯿﻘﺤﺗ مﺎﺠﻧا زﺎﺳ
.ﺪﺷﺎﺑ ﻪﻨﯿﻣز شور ﺳﺎﻨﺷ ﯽ ﺶﻫوﮋﭘ
ﻦﯾا رد ﻖﯿﻘﺤﺗ ﯽﺑﺮﺠﺗ ﻪﮐ نآ ﻞﮑﺗوﺮﭘ ﻂﺳﻮﺗ
ﺘﯿﻤﮐ ﮥ قﻼﺧا هﺎﮕﺸﻧاد ﺮﺒﺗ ﯾﺰ ﺎﺑ ﺳﺎﻨﺷ ﮥ
IR.TABRIZU.REC.1399.054
ﺄﺗ ﯿﯾ ﺪ ،ﺪﺷ 24 ﺮﺳ
ﯽﯾاﺮﺤﺻ شﻮﻣ داﮋﻧ ﺮﻧ
رﺎﺘﺴﯾو 16 ﻪﺘﻔﻫ يا نزو ﺎﺑ 300 ﺎﺗ 370 ﻖﺒﻃ و اﺬﻏ و بآ ﻪﺑ دازآ ﯽﺳﺮﺘﺳد ﺎﺑ ﺲﻔﻗ ﺮﻫ رد ﺮﺳ ﻪﺳ داﺪﻌﺗ ﻪﺑ ﻪﮐ مﺮﮔ
ﺧﺮﭼ ﮥ 12 رد يراﺪﯿﺑ و باﻮﺧ ﺖﻋﺎﺳ ناﻮﯿﺣ
ﻧﺎﺧ ﮥ ﺪﮑﺸﻧاد ة ﯽﻣ يراﺪﻬﮕﻧ ﺰﯾﺮﺒﺗ ﯽﮑﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ﯽﮑﺷﺰﭙﻣاد ﺪﻧﺪﺷ
، ﻪﺑ ﻪﺳ ﻪﺑ ﯽﻓدﺎﺼﺗ رﻮﻃ
) هوﺮﮔ ﺖﺸﻫ ﺳ ،لﺮﺘﻨﮐ ﻢﻟﺎﺳ ﻞﻣﺎﺷ (ﯽﯾﺎﺗ ﻦﯾﺮﻤﺗ ﻪﺘﮑﺳ ،لﺮﺘﻨﮐ ﻪﺘﮑ
ﺴﻘﺗ ﯿ ﻢ و ﺪﻧﺪﺷ ﻪﺑ ،لﺮﺘﻨﮐ ﻢﻟﺎﺳ هوﺮﮔ زا ﺮﯿﻏ ﯿﻘﺑ
ﮥ شﻮﻣ ﺎﻫ ي ﯽﯾاﺮﺤﺻ
ﻞﻣﺎﺷ 16 ﻖﯾرﺰﺗ ﺖﺤﺗ ﯽﻟاﻮﺘﻣ زور ود ﯽﻃ ﺮﺳ نورد
زود ﺎﺑ لﻮﻧﺮﺗوﺮﭘوﺰﯾا ﯽﻗﺎﻔﺻ 100
ﯽﻠﯿﻣ راﺮﻗ زور رد نﺪﺑ نزو مﺮﮔﻮﻠﯿﮐ ﺮﻫ ﺮﺑ مﺮﮔ
ﺎﺑ ﻪﺑﺎﺸﻣ ،لﻮﻧﺮﺗﺮﭘوﺰﯾا ﻖﯾرﺰﺗ زا ﺪﻌﺑ زور ود .ﺪﻨﺘﻓﺮﮔ تﺎﻌﻟﺎﻄﻣ
ﻪﺘﺷﺬﮔ ) 34 ( زا ﺷﻮﮔ ﮥ ﻢﺸﭼ هوﺮﮔ ﺎﻫ ي ﻪﺘﮑﺳ لﺮﺘﻨﮐ و ﻢﻟﺎﺳ لﺮﺘﻨﮐ ﻧﻮﻤﻧ
ﮥ ﻧﻮﺧ ﯽ
و ﺪﺷ ﺬﺧا ﺄﺗ
ﯿﯾ ﺪ ﻖﯾﺮﻃ زا ﻪﺘﮑﺳ زوﺮﺑ ﺴﯾﺎﻘﻣ
ﮥ ﻦﯿﺑ حﻮﻄﺳ ﯽﻫوﺮﮔ ﺖﯿﻟﺎﻌﻓ ﻦﯿﻨﭽﻤﻫ و ﻦﯿﻧﻮﭘوﺮﺗ
ﺰﻧآ ﯾ ﻢ ﺎﻫ ي ﻦﯿﺗاﺮﮐ و زﺎﻨﯿﮐ تﺎﺘﮐﻻ ﻫد ﯿ زﺎﻧژورﺪ
مﺎﺠﻧا نﻮﺧ شدﺮﮔ ﯽﺒﻠﻗ ﺖﻓﺮﮔ
.
1. 8-hydroxy-2' -deoxyguanosine (8-OHdG) or 8-oxo-7,8-dihydro-2' -deoxyguanosine (8-oxodG)
2. Cabonylated proteins
3. Carbonylated Proteine
4. Ventricular Arrhythmias
20
ﺰﯿﯾﺎﭘ ،مﻮﺳ ةرﺎﻤﺷ ،ﻢﻫدرﺎﻬﭼ ةرود ،ﯽﺷزرو ﯽﺘﺴﯾز مﻮﻠﻋ 1401
لوﺪﺟ 1 . ﺖﯿﻟﺎﻌﻓ ﺰﻧآ ﯾ ﻢ ﺎﻫ ي ﯽﺒﻠﻗ ﻪﺑ رﻮﻈﻨﻣ ﺄﺗ ﯿﯾ ﺪ زوﺮﺑ ا ﺎﻔﻧ ﻣ سﻮﺘﮐر ﯿ درﺎﮐﻮ
زﺎﻨﯿﮐ ﻦﯿﺗاﺮﮐ ﺖﯿﻟﺎﻌﻓ هوﺮﮔ ﺪﺣاو) ﺑﯿ ﻦ ﻠﻠﻤﻟا ﯽ (ﺮﺘﯿﻟ ﺮﺑ ﺖﯿﻟﺎﻌﻓ
تﺎﺘﮐﻻ زﺎﻧژورﺪﯿﻫد
ﺪﺣاو) ﺑﯿ ﻦ ﻠﻠﻤﻟا ﯽ (ﺮﺘﯿﻟ ﺮﺑ ﻦﯿﻧﻮﭘوﺮﺗ
ﺮﺑ مﺮﮔﻮﻧﺎﻧ) ﻣﯿ
ﻠ ﯽ ﻟﯿ ﺮﺘ (
ﻢﻟﺎﺳ لﺮﺘﻨﮐ 20
/ 16 37 ± / 286 06
/ 13 75 ± / 236 10
/ 0 ۷۷ ± ۰/
ﻪﺘﮑﺳ لﺮﺘﻨﮐ 51
/ 16 5 ± / 324 07
/ 16 87 ± / 286 12
/ 0 ۹۰ ± ۰/
ﯽﻫوﺮﮔ ﻦﯿﺑP
001 / 0
* 001
/ 0
* 039
/ 0
*
:*
توﺎﻔﺗ رادﺎﻨﻌﻣ ) 05 / 0
<
.(P
شﻮﻣ ﻪﻣادا رد ﯽﯾاﺮﺤﺻ يﺎﻫ
هوﺮﮔ ﻪﺘﮑﺳ و ﻦﯾﺮﻤﺗ ﻪﺘﮑﺳ ﻞﻣﺎﺷ ﻦﯾﺮﻤﺗ يﺎﻫ مأﻮﺗ
ﺷزرو ﻞﮑﺗوﺮﭘ ﯽ
ﯽﻃ ﮏﯾ ﻪﺘﻔﻫ ﻞﻣﺎﺷ) 5 ﻪﺴﻠﺟ نﺪﯾود
يور نادﺮﮔ راﻮﻧ ﺎﺑ
ﺖﻋﺮﺳ 5 ﺎﺗ 10 ﻗد ﺮﺑ ﺮﺘﻣ ﯿ ﻪﻘ .ﺪﻧﺪﺷ ﺎﻨﺷآ ( ﻦﯾﺪﺑ
شﻮﻣ رﻮﻈﻨﻣ ﺻ يﺎﻫ
ود رد ﯽﯾاﺮﺤ ﺴﻠﺟ
ﮥ تﺪﻣ ﻪﺑ لوا 10
رد ﻂﻘﻓ ﻪﻘﯿﻗد
ﻞﺧاد نادﺮﮔ راﻮﻧ ﻪﺳ رد ؛ﺪﻨﺘﻓﺮﮔ راﺮﻗ
ﺴﻠﺟ ﮥ يﺪﻌﺑ ﻪﺑ رﺪﺗ ﯾ ﺞ ود رد ﻪﮐ ﺪﻧدﺮﮐ ﺖﯿﻟﺎﻌﻓ ﻪﺑ عوﺮﺷ ﺴﻠﺟ
ﮥ ﺐﯿﺷ ﺎﺑ ﯽﻧﺎﯾﺎﭘ 5
ﺎﺑ و دﻮﺑ هاﺮﻤﻫ ﺪﺻرد
ﺖﻋﺮﺳ 5 ﺎﺗ 10 مﺎﺠﻧا ﻪﻘﯿﻗد رد ﺮﺘﻣ ﺖﻓﺮﮔ
ﺲﭙﺳ . ﻣﺎﻧﺮﺑ ﮥ ﺮﻤﺗ ﯾ ﻦ تﺪﻣ ﻪﺑ 8 ﻪﺘﻔﻫ ﻪﮐ ﺪﺷ زﺎﻏآ 5
د زور ﯽﻟاﻮﺣ ﻪﺘﻔﻫ ر ﺖﻋﺎﺳ
16 مﺎﺠﻧا ،
ﺖﻓﺮﮔ ود رد نﺪﯾود . ﺘﻔﻫ
ﮥ ﺖﻋﺮﺳ ﺎﺑ لوا 10
تﺪﻣ ﻪﺑ ﻪﻘﯿﻗد رد ﺮﺘﻣ 15
مﺎﺠﻧا ﻪﻘﯿﻗد ﺖﻓﺮﮔ
ﻪﺘﻔﻫ رد . يﺪﻌﺑ يﺎﻫ ﻪﺑ
رﺪﺗ ﯾ ﺞ تﺪﺷ ﻪﺘﻔﻫ ود ﺮﻫ
ﻪﮑﻨﯾا ﺎﺗ ،ﺪﺷ هداد ﺶﯾاﺰﻓا ﻦﯾﺮﻤﺗ تﺪﻣ و رد
ود ﺘﻔﻫ ﮥ ﻪﺑ يزاﻮﻫ ﻦﯾﺮﻤﺗ تﺪﺷ ﺮﺧآ 25
ﻪﺑ ﺖﯿﻟﺎﻌﻓ تﺪﻣ و ﻪﻘﯿﻗد رد ﺮﺘﻣ 30
ﻗد ﺐﯿﺷ .ﺪﯿﺳر ﻪﻘﯿ
يﺎﻬﺘﻧا ﺎﺗ اﺪﺘﺑا زا ﻞﯿﻣدﺮﺗ رود
ة ﻦﯾﺮﻤﺗ 5 لوﺪﺟ) ﺪﻧﺎﻣ ﺖﺑﺎﺛ ﺪﺻرد 2
.(
نوﺰﻓا ﻦﯾا ﺮﺑ ﺮﻫ رد ، ﺴﻠﺟ ﮥ ،ﻦﯾﺮﻤﺗ 5 ﺎﺑ ندﺮﮐ دﺮﺳ و ندﺮﮐ مﺮﮔ ﻪﻘﯿﻗد
ﺖﻋﺮﺳ 5 ﺎﺗ 10 مﺎﺠﻧا ﻪﻘﯿﻗد رد ﺮﺘﻣ ﺖﻓﺮﮔ
) 35 (.
لوﺪﺟ 2 . ﺋﺰﺟ ﯿ تﺎ ﻣﺎﻧﺮﺑ ﮥ ﺮﻤﺗ ﯾ ﻦ ﻃ ﯽ رود ة ﻪﻠﺧاﺪﻣ
،ﻪﻠﺧاﺪﻣ نﺎﯾﺎﭘ زا ﺲﭘ شﻮﻣ
ﺎﻫ ي ﺻ ﯽﯾاﺮﺤ ) ﻦﯿﻣﺎﺘﮐ ﺎﺑ
mg/kg
150 ) ﻦﯾزﻼﯾاز و (
mg/kg
15 ( ﺑ ﯽ شﻮﻫ .ﺪﻧﺪﺷ ﺢﯾﺮﺸﺗ و ﻧﻮﻤﻧ
ﮥ نﻮﺧ
هزاﺪﻧا ياﺮﺑ ﻪﺘﺨﻟ راﺪﻘﻣ يﺮﯿﮔ
ﻊﻤﺟNOX4
روآ ي ﺪﺷ ﻖﯾرﺰﺗ زا ﺲﭘ ﻪﯿﻠﮐ ﺖﻓﺎﺑ ﺲﭙﺳ . ﺮﺳPBS
زا ﺲﭘ ﻪﻣادا رد و ﺪﺷ يزﺎﺴﮐﺎﭘ نﻮﺧ زا ،د
تﺎﻌﻄﻗ ﻪﺑ ﻢﯿﺴﻘﺗ ﮏﭼﻮﮐ
ﺮﺗ ﻞﺧاد رد 5 - 10 ﺮﺘﯿﻟ ﯾ ﺎﺑ هﺪﺷ دﺮﺳPBS
رﺰﯾﺎﻧژﻮﻤﻫ ﻂﺳﻮﺗ ﺦ
(SR30 model ps 80)
ﻪﺑ ترﻮﺻ رد نژﻮﻤﻫ
لﺎﭽﺨﯾ ژﻮﯿﻔﯾﺮﺘﻧﺎﺳ رد ﯽﯾﺎﻬﻧ نﻮﯿﺴﻧﺎﭙﺳﻮﺳ .ﺪﻣآ تﺪﻣ ﻪﺑ راد
5 ﺖﻋﺮﺳ ﺎﺑ ﻪﻘﯿﻗد 5000
راﺮﻗ ﮔ ﯽﺑﺎﯾزرا نﺎﻣز ﺎﺗ ﯽﯾﺎﻬﻧ نﺎﺗﺎﻧﺮﭘﻮﺳ ﺲﭙﺳ و ﺖﻓﺮ
يﺎﻣد رد 80 - .ﺪﺷ ﺪﻤﺠﻨﻣ
يد نﻮﻟﺎﻣ راﺪﻘﻣ ﺪﯿﻫﺪﻟآ
) (MDA
ﻪﺑ ناﻮﻨﻋ ﭙﯿﻟ نﻮﯿﺳاﺪﯿﺴﮐاﺮﭘ ﺺﺧﺎﺷ يﺪﯿ
بﺎﺗزﺎﺑ ﺪﻨﻫد ة ﯽﺸﯾﺎﺴﮐا سﺮﺘﺳا راﺪﻘﻣ ﺑﺮﭼ
ﯽ ﺎﻫ زا هدﺎﻔﺘﺳا ﺎﺑ (
شور ﮓﻧر ) ﻦﯿﭼ ﺖﺧﺎﺳ هﮋﯾو ﺖﯿﮐ ﻂﺳﻮﺗ ﯽﺠﻨﺳ
MDA; Biodiagnostic, Cat Number: MD 2529
ﺖﮐﺮﺷ ﻞﻤﻌﻟارﻮﺘﺳد ﻖﺒﻃ و (
رد رﺪﯾر هﺎﮕﺘﺳد ﻂﺳﻮﺗ هﺪﻧزﺎﺳ ﻃ
ﯿ ﻒ 532 هزاﺪﻧا ﺮﺘﻣﻮﻧﺎﻧ ﺪﺷ يﺮﯿﮔ
) 36 ( .
ﺺﺧﺎﺷ ﻪﺘﻔﻫ
ﺎﻨﺷآ ﯽﯾ لوا مود مﻮﺳ مرﺎﻬﭼ ﻢﺠﻨﭘ ﻢﺸﺷ ﻢﺘﻔﻫ ﻢﺘﺸﻫ
ﺖﻋﺮﺳ ﺮﺘﻣ) رد ﻗد ﯿ ﻪﻘ ( 5-10 10 10 15 15 20 20 25 25
تﺪﻣ ﻗد) ﯿ ﻪﻘ ( 10
15 15 20 20 25 25 30 30
ﺷ ﯿ ﺐ (ﺪﺻرد) 5
5 5 5 5 5 5 5 5
ﺮﻤﺗ تاﺮﯿﺛﺄﺗ ﯾ
ﻦ زاﻮﻫ ي ﺺﺧﺎﺷ ﺮﺑ ﺎﻫ ي ﺎﺴﮐا سﺮﺘﺳا ﯾ
ﺸ ﯽ ﻠﮐ ﯿ ﻪ راﺪﻘﻣ و شﻮﻣ مﺮﺳNOX4
ﺎﻫ ي اﺮﺤﺻ ﯽﯾ ﯽﺒﻠﻗ ﮥﺘﮑﺳ لﺪﻣ
21
) ﻪﻠﯿﻧﻮﺑﺮﮐ ﻦﯿﺌﺗوﺮﭘ ﺖﻈﻠﻏ ﻪﺑ (CP
ﻦﯿﺌﺗوﺮﭘ ﺢﻄﺳ ناﻮﻨﻋ يﺎﻫ
ﺶﯾﺎﺴﮐا ﺘﻓﺎﯾ ﮥ يﺮﺘﻣﻮﺘﻓوﺮﺘﮑﭙﺳا ﻂﺳﻮﺗ ،ﯽﺸﯾﺎﺴﮐا سﺮﺘﺳا زا ﯽﺷﺎﻧ
ﻒﯿﻃ) زا هدﺎﻔﺘﺳا ﺎﺑ و (ﯽﺠﻨﺳ دﺎﻣ
ة 2 و 4 ﻦﯾزارﺪﯿﻫ ﻞﯿﻨﻓوﺮﺘﯿﻧ يد هزاﺪﻧا1
ﺶﻨﮐاو ﻞﯿﻧﻮﺑﺮﮐ تﺎﻘﺘﺸﻣ .ﺪﺷ يﺮﯿﮔ ﺐﯾﺮﺿ زا هدﺎﻔﺘﺳا ﺎﺑ ﻪﺘﻓﺎﯾ
ﻒﯿﻃ ردDPNH
370 ) ﺮﺘﻣﻮﻧﺎﻧ
22×103 L/mol/cm
هزاﺪﻧا ( ﯽﻠﯿﻣ ﺮﺑ لﻮﻣﻮﻧﺎﻧ ﺐﺴﺣﺮﺑ و ﺪﺷ يﺮﯿﮔ نﺎﯿﺑ ﻦﯿﺌﺗوﺮﭘ مﺮﮔ
ﺪﺷ ) 37 (.
ياﺮﺑ هزاﺪﻧا ﮔﯿ ﺮ ي راﺪﻘﻣ
8-OHdG
ﺖﻓﺎﺑ ﯿﻠﮐ ﮥ هزﺎﺗ يزﺎﺳاﺪﺟ رد هﺪﺷ 5 ﻣﯿ ﻠ ﯽ ﻟﯿ ﺮﺘ ﺖﻈﻠﻏ ﺎﺑ ﺲﯾﺮﺗ ﺮﻓﺎﺑ 50
ﯽﻠﯿﻣ لﻮﻣ ) ﺮﺘﯿﻟ ﺮﺑ 4/
7
= (pH
ﺲﭙﺳ .ﺪﺷ ﺰﯾﺎﻧژﻮﻤﻫ ﻪﻧﻮﻤﻧ
نژﻮﻤﻫ يﺎﻫ 800
ﻪﻘﯿﻗد رد رود تﺪﻣ ﻪﺑ
10 ﻪﻘﯿﻗد ﺎﺗ ﻔﯾﺮﺘﻧﺎﺳ ﻪﺘﺴﻫ بﻮﺳر ﯿ
و ﺪﻧﺪﺷ ژﻮ ﮏﯾ ﺲﭙﺳ
ﻣﯿ ﻠ ﯽ ﻟﯿ ﺮﺘ
لﻮﻠﺤﻣ يوﺎﺣ 10 ﯽﻠﯿﻣ لﻮﻣ ﺮﺑ ﺮﺘﯿﻟ ،EDTA
10 ﯽﻠﯿﻣ ) ﺲﯾﺮﺗ ﺮﻓﺎﺑ ﺮﺘﯿﻟ ﺮﺑ لﻮﻣ 8
= ،(pH
150 ﯽﻠﯿﻣ لﻮﻣ ﺮﺘﯿﻟ ﺮﺑ وNaCl
ود ﺖﻈﻠﻏ ﺎﺑSDS
تﺪﻣ ﻪﺑ طﻮﻠﺨﻣ ﺲﭙﺳ .ﺪﺷ ﻪﻓﺎﺿا ﺪﺻرد 70
يﺎﻣد رد ﻪﻘﯿﻗد 56
ﺟرد ﮥ ﺴﻠﺳ ﯿ سﻮ زﺎﻨﯿﺌﺗوﺮﭘ ﺎﺑ )K
700 ﺮﺑ مﺮﮔوﺮﮑﯿﻣ ﻣﯿ
ﻠ ﯽ ﻟﯿ ﺮﺘ و ﺪﺷ ﻪﺑﻮﮑﻧا (
تﺪﻣ ﻪﺑ ﺲﭙﺳ 10
يﺎﻣد رد ﻪﻘﯿﻗد 95
ﺟرد ﮥ سﻮﯿﺴﻠﺳ ﻪﻣادا رد .ﺪﺷ هداد تراﺮﺣ
ﻢﺠﺣ ﺎﺑ DNA
ﻞﯿﻣآوﺰﯾا و مﺮﻓوﺮﻠﮐ ،لﻮﻨﻓ يوﺎﺴﻣ يﺎﻫ
) ﻞﮑﻟا
25 : 24 : 1
ﺲﭙﺳ .ﺪﺷ ﻪﻓﺎﺿا مﺮﻓوﺮﻠﮐ هرﺎﺑود ﺲﭙﺳ و ﺪﺷ يزﺎﺳاﺪﺟ ( يﺎﻣد ردDNA
20 - ﻞﺧاد رد ﺖﻋﺎﺳ ود تﺪﻣ ﻪﺑ سﻮﯿﺴﻠﺳ
لﻮﻧﺎﺗا 70 ﻪﻣادا رد .ﺪﺷ هداد بﻮﺳر ﺪﺻرد ) ﺲﯾﺮﺗ ﺮﻓﺎﺑ ﻞﺧاد رد هرﺎﺑود DNA
10 ﻠﯿﻣ ﯽ لﻮﻣ ﺎﺑ هاﺮﻤﻫ (ﺮﺘﯿﻟ ﺮﺑ )EDTA
1/
0 ﯽﻠﯿﻣ لﻮﻣ ﺮﺑ
ﺎﺑ (ﺮﺘﯿﻟ 8
= ﻖﯿﻠﻌﺗpH
ﺪﺷ ) تﺎﺘﺳا ﻢﯾﺪﺳ ﺮﻓﺎﺑ ﺮﺘﯿﻟوﺮﮑﯿﻣ ﺞﻨﭘ . 200
ﯽﻠﯿﻣ لﻮﻣ ﺎﺑ (ﺮﺘﯿﻟ ﺮﺑ 8/
4
= وpH
5 زﺎﺌﻠﮐﻮﻧ مﺮﮔوﺮﮑﯿﻣ )P1
Invitrogen,
CA, USA
ﻪﺑ ( 45 ﻪﻧﻮﻤﻧ زا ﺮﺘﯿﻟوﺮﮑﯿﻣ يﺎﻫ
يﺎﻣد رد ﺖﻋﺎﺳ ﮏﯾ تﺪﻣ ﻪﺑ طﻮﻠﺨﻣ .ﺪﺷ ﻪﻓﺎﺿا DNA
37 رد ﺟ ﮥ سﻮﯿﺴﻠﺳ ﺎﺗ ﺪﺷ ﻪﺑﻮﮑﻧا
ﺖﻈﻠﻏ ﺎﺑ ﺲﯾﺮﺗ ﺮﻓﺎﺑ ﺮﺘﯿﻟوﺮﮑﯿﻣ ﺞﻨﭘ ﺲﭙﺳ .دﻮﺷ ﻪﺘﺴﮑﺷ ﺎﻫﺪﯿﺗﻮﻠﮐﻮﻧ ﻪﺑDNA
500 ﯽﻠﯿﻣ لﻮﻣ ) ﺮﺘﯿﻟ ﺮﺑ 8
= ،(pH
10 ﯽﻠﯿﻣ لﻮﻣ يد ﺮﺘﯿﻟ ﺮﺑ
و ﻢﯾﺰﯿﻨﻣ ﺪﯾﺮﻠﮐ 6/
0 ﻪﺑ زﺎﺗﺎﻔﺴﻓ ﻦﯿﻟﺎﮑﻟآ ﺪﺣاو ﻪﻧﻮﻤﻧ
ﺎﻫ يﺎﻣد رد ﺖﻋﺎﺳ ﮏﯾ تﺪﻣ ﻪﺑ طﻮﻠﺨﻣ هرﺎﺑود .ﺪﺷ ﻪﻓﺎﺿا 37
ﺟرد ﮥ سﻮﯿﺴﻠﺳ ﮑﻧا
ﻪﺑﻮ
ﺪﻧﻮﺷ ﻪﺘﺴﮑﺷ ﺪﯾزﻮﺌﻠﮐﻮﻧ ﻪﺑ ﺎﻫﺪﯿﺗﻮﻠﮐﻮﻧ ﺎﺗ ﺪﺷ )
38 ( ﻪﻧﻮﻤﻧ ﺖﯾﺎﻬﻧ رد . راﺪﻘﻣ ﻦﯿﯿﻌﺗ ياﺮﺑ ﺪﯾزﻮﺌﻠﮐﻮﻧ يﺎﻫ
8-OHdG
ﺖﺧﺎﺳ اﺰﯾﻻا ﺖﯿﮐ ﻂﺳﻮﺗ
ﻦﯿﭼ ﻮﯿﺑازﻮﮐ ﺖﮐﺮﺷ
CSB-E10140h
ﺖﯿﺳﺎﺴﺣ ﺎﺑ هﺪﻧزﺎﺳ ﺖﮐﺮﺷ ﻞﻤﻌﻟارﻮﺘﺳد ﻖﺒﻃ و 12
/3 ﺮﺑ مﺮﮔﻮﻧﺎﻧ ﻣﯿ
ﻠ ﯽ ﻟﯿ ﺮﺘ ﻒﯿﻃ رد و 450
ﺮﺘﻣﻮﻧﺎﻧ
ﺷ هدﺎﻔﺘﺳا ﺪﻧﺪ ) 39 (.
ﻧﻮﻤﻧ ﮥ تﺪﻣ ﻪﺑ اﺪﺘﺑا ﺬﺧا زا ﺲﭘ ﯽﻄﯿﺤﻣ نﻮﺧ 20
ﺎﺑ ﻪﻘﯿﻗد ﺘﻧآ ﯽ دﺎﺑ ي تﺪﻣ ﻪﺑ ﺲﭙﺳ و (نﺎﻤﻟآ ﭻﯾرﺪﻟآ ﺎﻤﮕﯿﺳ) NOx4
30 ﺎﺑ ﻪﻘﯿﻗد
ﺘﻧآ ﯽ دﺎﺑ ي ﯾﻮﻧﺎﺛ ﮥ ﻪﮔوﮋﻧﻮﮐ هﺪﺷ تﺎﻧﺎﯿﺳﻮﯿﺗوﺰﯾا ﺎﺑ2
ﺖﺴﺷ رﺎﺑ ﻪﺳ زا ﺪﻌﺑ ﻪﻧﻮﻤﻧ ﺲﭙﺳ .ﺪﺷ ﻪﺑﻮﮑﻧا (نﺎﻤﻟآ ﭻﯾرﺪﻟآ ﺎﻤﮕﯿﺳ)3
و ﺎﺑ ﻮﺷ ،PBS
ﻔﯾﺮﺘﻧﺎﺳ ﯿ ژﻮ ﺪﻧﺪﺷ ﺪﺟ مﺮﺳ و يزﺎﺳا
ﺪﺷ لﻮﮑﻟﻮﻣ ﺖﯾﺎﻬﻧ رد . مﺮﺳ ردNOX4
ﻪﺑ ﺖﺳد هزاﺪﻧا ﺮﺘﻣﻮﺘﯿﺳﻮﻠﻓ هﺎﮕﺘﺳد ﻂﺳﻮﺗ هﺪﻣآ ﺪﺷ يﺮﯿﮔ
) 40 ( .
شور رﺎﻣآ ي
ﺲﭘ زرا زا ﯾﺑﺎ ﯽ زﻮﺗ ﯾﻊ ﺒﻃ ﯿﻌ ﯽ هداد ﺎﻫ ﻪﺑ ﮏﻤﮐ نﻮﻣزآ ﭘﺎﺷ ﯿ وﺮ و ﯾ
،ﮏﻠ رد
،ﻪﻣادا اﺮﺑ ي ﺎﻘﻣ ﯾ ﺴ ﮥ ﺑﯿ ﻦ ﻫوﺮﮔ ﯽ هداد ﺎﻫ زا ﻠﺤﺗ ﯿ ﻞ راو ﯾ ﺲﻧﺎ ﺮﮑﺗ ا ﻪﻫ
هدﺎﻔﺘﺳا ﺪﺷ ﻪﮐ رد ترﻮﺻ رادﺎﻨﻌﻣ نﺪﺷ
،نآ رد ﻪﻣادا هداد ﺎﻫ ﺎﺑ هدﺎﻔﺘﺳا زا نﻮﻣزآ ﻘﻌﺗ ﯿﺒ ﯽ ﮐﻮﺗ و ﯽ ﯾ لوﺎﻫ ﺰﻤﯿﺟ ﺎ ﻪﺘﺴﺑ)
ﻪﺑ ﺎﺘﻧ ﯾ ﺞ نﻮﻣزآ ﻮﻟ ن (
ﺎﻘﻣ ﯾ ﻪﺴ ﺪﻧﺪﺷ . مﺎﻤﺗ نﻮﻣزآ ﺎﻫ ﺎﺑ مﺮﻧ راﺰﻓا ﺨﺴﻧSPSS
ﮥ ﻤﻃا ﺢﻄﺳ رد21
ﯿ نﺎﻨ رﺎﻣآ ي ﻠﺤﺗ ﺪﺻرد95
ﯿ ﻞ .ﺪﻧﺪﺷ
ﯾ ﻪﺘﻓﺎ ﺎﻫ ي ﺶﻫوﮋﭘ
ﺑ و ﺐﻠﻗ نزو نﺪ
شﻮﻣ ﺎﻫ لوﺪﺟ رد رﺎﺘﺸﮐ نﺎﻣز رد 3
.ﺖﺳا هﺪﺷ ﻪﺋارا
لوﺪﺟ 3 . هوﺮﮔ نﺪﺑ و ﺐﻠﻗ نزو رﺎﺘﺸﮐ مﺎﮕﻨﻫ ﺎﻫ
نﺪﺑ نزو هوﺮﮔ (مﺮﮔ) ﺐﻠﻗ نزو
(مﺮﮔ)
لﺮﺘﻨﮐ ﻢﻟﺎﺳ 18
/ 27 78± / 300 11
/0 1± /
2 /93± /021 334/06±16/54 لﺮﺘﻨﮐ ﻪﺘﮑﺳ 1 /91± /022 338/20±16/04 ﻦﯾﺮﻤﺗ ﻪﺘﮑﺳ 1
1. Dinitrophenylhydrazine
2. Congogated Antibody
3. Isothiosyanate- Isothiocyanate
22
ﺰﯿﯾﺎﭘ ،مﻮﺳ ةرﺎﻤﺷ ،ﻢﻫدرﺎﻬﭼ ةرود ،ﯽﺷزرو ﯽﺘﺴﯾز مﻮﻠﻋ 1401
ﺎﺘﻧ ﯾ ﺞ ﻠﺤﺗ ﯿ ﻞ راو ﯾ ﺲﻧﺎ درﻮﻣ رد ﻪﻫاﺮﮑﺗ ﺎﻘﻣ
ﯾ ﺴ ﮥ ﺑﯿ ﻦ ﻫوﺮﮔ ﯽ راﺪﻘﻣ مﺮﺳ NOx4
) 001 /0
=
، P
79 / 48
=
F2, 21
( و راﺪﻘﻣ
MDA
) 001 /0
=
، P
76 / 191
=
F2, 21
(،
ﺌﺗوﺮﭘ ﯿ ﻦ ﻧﻮﺑﺮﮐ ﯿ ﻪﻠ ) 001 /0
=
، P
49 / 45
=
F2, 21
و ( )OHdG
001 / 0
=
، P
95 / 121
=
F2, 21
( ﻠﮐ ﯿﻪ نﺎﺸﻧ
داد ﻪﮐ توﺎﻔﺗ ﺎﻫ ي رادﺎﻨﻌﻣ ي رد ﺑﯿ ﻦ هوﺮﮔ ﺎﻫ دﻮﺟو دراد ) ﻞﮑﺷ ﺎﻫ ي 1 - 4 .(
ﻞﮑﺷ 1 راﺪﻘﻣ . شﻮﻣ رد مﺮﺳNOX4
لﺪﻣ يﺎﻫ ﺘﮑﺳ ﮥ دﺎﺣ ﯽﺒﻠﻗ
و * توﺎﻔﺗ ﺮﮕﻧﺎﯾﺎﻤﻧ :† رادﺎﻨﻌﻣ
لﺮﺘﻨﮐ ﻪﺘﮑﺳ و لﺮﺘﻨﮐ ﻢﻟﺎﺳ هوﺮﮔ ﻪﺑ ﺖﺒﺴﻧ )
05 / 0
<
(P
ﻞﮑﺷ 2 راﺪﻘﻣ . ﯿﻠﮐMDA
ﮥ شﻮﻣ لﺪﻣ يﺎﻫ ﯽﺒﻠﻗ ﮥﺘﮑﺳ دﺎﺣ
و * توﺎﻔﺗ ﺮﮕﻧﺎﯾﺎﻤﻧ :† رادﺎﻨﻌﻣ
ﻪﺘﮑﺳ و لﺮﺘﻨﮐ ﻢﻟﺎﺳ هوﺮﮔ ﻪﺑ ﺖﺒﺴﻧ لﺮﺘﻨﮐ
) 05 / 0
<
( P
ﻞﮑﺷ 3 راﺪﻘﻣ . شﻮﻣ ﻪﯿﻠﮐCP
لﺪﻣ يﺎﻫ ﯽﺒﻠﻗ ﮥﺘﮑﺳ دﺎﺣ
و * توﺎﻔﺗ ﺮﮕﻧﺎﯾﺎﻤﻧ :† رادﺎﻨﻌﻣ
لﺮﺘﻨﮐ ﻪﺘﮑﺳ و لﺮﺘﻨﮐ ﻢﻟﺎﺳ هوﺮﮔ ﻪﺑ ﺖﺒﺴﻧ )
05 / 0
<
( P
