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References
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8. Khalili M, Shahabi-nejad N, Golchin M. Q fever serology in febrile patients in Southeast Iran. Trans Roy Soc Trop Med Hyg. 2010; 104: 623-624. (In Persian) 9. Khalili M, Sakhaee E, Aflatoonian MR,
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17.Borji S, Jamshidi A, Khanzadi S, Razmyar J. Detection of coxiella burnetii and sequencing the IS1111 gene fragment in bulk tank milk of dairy herds. Iran J Vet Sci Tech. 2014; 6 (2): 21-28. (In Persian) 18.Khademi P, Jaydari A, Esmaeili M.
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19.Khademi P, Mahzounieh M, Esmaeili Kotahmer M. Genomic Detection of Coxiella burnetii in Cattle Milk Samples by Nested - PCR method in Bonab, Iran. Arak Med Univ J. 2015; 18(97): 49-57. (In Persian)
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Detection of coxiella burnetii in cows, goats, and ewes bulk milk samples using polymerase chain reaction (PCR).
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25.Engelen E, Schotten N, Schimmer B, Hautvast JL, van Schaik G, van Duijnhoven YT. Prevalence and risk factors for Coxiella burnetii (Q. fever) in Dutch dairy cattle herds based on bulk tank milk testing. Prev Vet Med. 2014; 117(1):
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2016; 1-8.
Genomic Detection of Coxiella burnetii in Raw and Unpasteurized Cow Milk of Traditional domestic dairy products Vendors in Khorramabad, Lorestan
Province in 2015
Etemadfar L1, Shams N*2, Jaidari A 2
1. MSc of Bacteriology, Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran.
2. Assistant Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, [email protected]
Received: 10 April 2017 Accepted: 2 May 2017
Abstract
Background: Q fever is a widespread zoonotic disease that is caused by obligate intracellular bacteria, Coxiella burnetii. Raw milk or dairy products that are produced from unpasteurized milk may contain virulent Coxiella burnetii. The objective of this study was to determine the prevalence rate of C. burnetii in raw and unpasteurized cow bulk tank milk samples of traditional domestic dairy products vendors in Khorramabad, Iran.
Materials and Methods: In this cross - sectional study a total of 120 raw and unpasteurized cow bulk tank milk samples in traditional domestic dairy products vendors were collected from October 2015 to November 2015 and tested for C. burnetii used a nested PCR assay.
Results: In this survey, 9 out of 120 (7.5%) raw and unpasteurized cow bulk tank milk samples were found PCR positive for C. burnetii.
Conclusion: The Results of this study indicate that raw and unpasteurized cow bulk tank milk samples in traditional domestic dairy products vendors are an important source of C. burnetii infection in Khorramabad.
Keywords: Nested PCR, Q - fever, Raw milk, Khorramabad.
*Citation: Etemadfar L, Shams N, Jaidari A. Genomic Detection of Coxiella burnetii in Raw and Unpasteurized Cow Milk of Traditional domestic dairy products Vendors in Khorramabad, Lorestan Province in 2015. Yafte. 2017; 19(2): 41-49.
