Identification of morphological and molecular Aspergillus species isolated from patients based on beta-tubulin gene sequencing

(1)

ﻪﺒﺗﺎﮑﻣ سردآ*

: وﺮﮔ ،ﯽﮑﺷﺰﭘ هﺪﮑﺸﻧاد ،نﺎﻬﻔﺻا ﯽﮑﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ،نﺎﻬﻔﺻا ﯽﺳﺎﻨﺷ چرﺎﻗ و ﻞﮕﻧا ه

.

ﺖﺴﭘ ﮏﯿﻧوﺮﺘﮑﻟا :

[email protected]

سﻮﻠﯾژﺮﭙﺳآ ﯽﻟﻮﮑﻟﻮﻣ و ﮏﯾژﻮﻟﻮﻓرﻮﻣ ﯽﯾﺎﺳﺎﻨﺷ نارﺎﻤﯿﺑ زا هﺪﺷاﺪﺟ يﺎﻫ

ﻦﯿﻟﻮﺑﻮﺗ ﺎﺘﺑ نژ ﯽﻟاﻮﺗ ﻦﯿﯿﻌﺗ سﺎﺳا ﺮﺑ )

β-tubulin

(

هاﻮﺧﺮﯿﺧ زﺎﻨﻬﻣ

،1

رﻮﭘ ﯽﻧﺎﮔدﺎﭼ ﯽﻔﻄﺼﻣ

،2

نﺎﻘﻫد ﻦﯾوﺮﭘ ،3

لﻮﺳر يﺪﻤﺤﻣ

* 4

-1 ﺪﺷرا سﺎﻨﺷرﺎﮐ هﺎﮕﺸﻧاد ،ﯽﮑﺷﺰﭘ هﺪﮑﺸﻧاد ،ﯽﮑﺷﺰﭘ ﯽﺳﺎﻨﺷ چرﺎﻗ و ﻞﮕﻧا هوﺮﮔ ،ﯽﮑﺷﺰﭘ ﯽﺳﺎﻨﺷ چرﺎﻗ

ناﺮﯾا ،نﺎﻬﻔﺻا ،نﺎﻬﻔﺻا ﯽﮑﺷﺰﭘ مﻮﻠﻋ .

-2 ،دﺎﺘﺳا ناﺮﯾا ،نﺎﻬﻔﺻا ،نﺎﻬﻔﺻا ﯽﮑﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ،ﯽﮑﺷﺰﭘ هﺪﮑﺸﻧاد ،ﯽﮑﺷﺰﭘ ﯽﺳﺎﻨﺷ چرﺎﻗ و ﻞﮕﻧا هوﺮﮔ .

3 - ،رﺎﯾدﺎﺘﺳا ناﺮﯾا ،نﺎﻬﻔﺻا ،نﺎﻬﻔﺻا ﯽﮑﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ،ﯽﮑﺷﺰﭘ هﺪﮑﺸﻧاد ،ﯽﮑﺷﺰﭘ ﯽﺳﺎﻨﺷ چرﺎﻗ و ﻞﮕﻧا هوﺮﮔ .

4 - ﺎﻨﺷ چرﺎﻗ و ﻞﮕﻧا هوﺮﮔ ،رﺎﯾدﺎﺘﺳا ناﺮﯾا ،نﺎﻬﻔﺻا ،نﺎﻬﻔﺻا ﯽﮑﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ،يﺮﯿﺴﻣﺮﮔ و ﯽﻧﻮﻔﻋ تﺎﻘﯿﻘﺤﺗ ﺰﮐﺮﻣ ،ﯽﮑﺷﺰﭘ هﺪﮑﺸﻧاد ،ﯽﮑﺷﺰﭘ ﯽﺳ

.

ﻪﺘﻓﺎﯾ / هرود زﻮﻧ ﻢﻫد / هرﺎﻤﺷ 1

/ رﺎﻬﺑ 96 / ﻞﺴﻠﺴﻣ 71

ﻪﻟﺎﻘﻣ ﺖﻓﺎﯾرد :

3 /11 / 95 شﺮﯾﺬﭘ

ﻪﻟﺎﻘﻣ : 19 /1 / 96

ﻪﻣﺪﻘﻣ :

ﻪﻧﻮﮔ يﺎﻫ ﺻﺮﻓ نژﻮﺗﺎﭘ ﻞﻣﺎﻋ سﻮﻠﯾژﺮﭙﺳآ داﺮﻓا رد ﺐﻠﻃ ﺖ

ياراد ﯽﻨﻤﯾا ﻢﺘﺴﯿﺳ ﻒﻌﺿ ﯽﻣ

ﺪﻨـﺷﺎﺑ . ﻦـﯾا چرﺎـﻗ ﺎـﻫ زا ﻪـﺒﻨﺟ يﺎـﻫ

ﺪﻨﺘﺴﻫ ﺖﯿﻤﻫا ﺰﺋﺎﺣ ﯽﯾاز ﻦﯿﺴﮐﻮﺗ و ﯽﯾاﺰﯾرﺎﻤﯿﺑ ﻒﻠﺘﺨﻣ .

ﻦﯿﯿﻌﺗ و ﺺﯿﺨﺸﺗ رد ﻦﯿﻟﻮﺑﻮﺗﺎﺘﺑ نژ ﯽﺑﺎﯾزرا ،ﺮﺿﺎﺣ ﻪﻌﻟﺎﻄﻣ فﺪﻫ ﻪـﻧﻮﮔ

يﺎـﻫ ﯽﻨﯿﻟﺎـﺑ

سﻮﻠﯾژﺮﭙﺳآ شور ﻪﺑ

،ﯽﻟاﻮﺗ ﻦﯿﯿﻌﺗ ﮏﯾژﻮﻟﻮﻓرﻮﻣ تﺎﯿﺻﻮﺼﺧ ﺎﺑ ﻪﺴﯾﺎﻘﻣ رد

) يﺪﯿﻧﻮﮐ ﻞﮑﺷ ﺪﻨﻧﺎﻣ ﺖـﺸﮐ رد ﯽﻨﻠﮐ ﻞﮑﺷ ،ﻢﯿﻘﺘﺴﻣ ﺶﯾﺎﻣزآ رد ﺎﻫ

و ﺖﺴﺗ يﺎﻫ ﮏﯾژﻮﻟﻮﯾﺰﯿﻓ (

ﯽﻣ ﺪﺷﺎﺑ .

شور و داﻮﻣ ﺎﻫ

:

ﻪﻌﻟﺎﻄﻣ ﻦﯾا رد 465

ﺑﯿ رﺎﻤ ﻪﻌﺟاﺮﻣ ﻪﺑ هﺪﻨﻨﮐ ﺪـﻨﺘﻓﺮﮔ راﺮـﻗ ﯽـﺳرﺮﺑ درﻮـﻣ ،نﺎﻬﻔـﺻا يﺎﻔـﺷ هﺎﮕـﺸﯾﺎﻣزآ .

ﯽﯾﺎـﺳﺎﻨﺷ

ﮏﯾژﻮﻟﻮﻓرﻮﻣ ﯽﻟﻮﮑﻟﻮﻣ و

ﻪﻧﻮﻤﻧ يﺎﻫ ﺐﯿﺗﺮﺗ ﻪﺑ ،ﯽﻨﯿﻟﺎﺑ هدﺎﻔﺘﺳا ﺎﺑ

زا يور ﺮﺑ ﺖﺸﮐ ﻂﯿﺤﻣ

يﺎﻫ و رﺎﮔآ ﺖﮐﺮﺘﺴﮐا ﺖﻟﺎﻣ ،رﺎﮔآ ورﻮﺑﺎﺳ رﺎـﮔآ ﺲﮑﭘﺎﭼ

،

ﯽﭘﻮﮑﺳوﺮﮑﯿﻣ هﺪﻫﺎﺸﻣ ﻪﻧﻮﻤﻧ

ﺎﻫ ﺖﯾﻮﻘﺗ و ﻦﯿﻟﻮﺑﻮﺗ ﺎﺘﺑ نژ ﻌﺗ و

ﯿﯿ ﻦ ﯽﻟاﻮﺗ مﺎﺠﻧا رﻮﮐﺬﻣ ﻪﯿﺣﺎﻧ ﺪﯾدﺮﮔ

. ﺲﻧﺎﮑﺳ ﺎﻫ تﺎـﻋﻼﻃا ﺎـﺑ ﻪـﺴﯾﺎﻘﻣ ﻖـﯾﺮﻃ زا

رد دﻮﺟﻮﻣ ﻧژ ﮏﻧﺎﺑ ﯽ ﺰﯿﻟﺎﻧآ ﺪﻧﺪﺷ .

ﻪﺘﻓﺎﯾ ﺎﻫ :

زا 465 هﺪﻨﻨﮐ ﻪﻌﺟاﺮﻣ ،كﻮﮑﺸﻣ

39 رﺎﻤﯿﺑ درﻮﻣ )

8

% ( ﺪﺷ ﯽﯾﺎﺳﺎﻨﺷ .

ﻦﯾﺮﺘﺸﯿﺑ ﻪﯾاﺪﺟ ﺎﻫ سووﻼﻓ ﻪﻧﻮﮔ ﻪﺑ طﻮﺑﺮﻣ )

56

% ( ، آﺰـﯾروا

) 20

% ( و سﻮﺗﺎﮕﯿﻣﻮﻓ )

10

% ( ﺪﻧدﻮﺑ . 59 % نز نارﺎﻤﯿﺑ و

41

% ﺪﻧدﻮﺑ دﺮﻣ .

ﻪﺠﯿﺘﻧ و ﺚﺤﺑ يﺮﯿﮔ

:

ﺎﺑ ﻪﺴﯾﺎﻘﻣ رد ﺖﺴﺗ

يﺎﻫ ،ﮏﯿﭙﯿﺗﻮﻨﻓ ﯽﯾﺎﺳﺎﻨﺷ ياﺮﺑ ﻦﯿﻟﻮﺑﻮﺗ ﺎﺘﺑ نژ ﯽﻟاﻮﺗ ﻦﯿﯿﻌﺗ

ﻪﯾاﺪﺟ ﺎﻫ ي سﻮﻠﯾژﺮﭙـﺳآ ﯽﻨﯿﻟﺎـﺑ

ﺖﺳا شزرا ﺎﺑ رﺎﯿﺴﺑ ﻪﻧﻮﮔ ﺢﻄﺳ رد .

ﯽﻨﯾﺰﮕﯾﺎﺟ شور

يﺎﻫ ﺎﺑ ﯽﻟﻮﮑﻟﻮﻣ شور

يﺎﻫ ﻖﯿﻗد ﺺﯿﺨﺸﺗ و ﮏﯿﭙﯿﺗﻮﻨﻓ ﻢﺴﯿﻧﺎﮔراوﺮﮑﯿﻣ

ﻪﺑ رﻮـﻈﻨﻣ نﺎـﻣرد

ﺮﺛﺆﻣ ﻪﯿﺻﻮﺗ ﻪﺻﺮﻋ ﻦﯾا نﺎﻘﻘﺤﻣ ﻪﺑ ﯽﻣ

ددﺮﮔ .

هژاو يﺪﯿﻠﮐ يﺎﻫ :

ﯽﻟاﻮﺗ ﻦﯿﯿﻌﺗ ،ﻦﯿﻟﻮﺑﻮﺗ ﺎﺘﺑ ،سﻮﻠﯾژﺮﭙﺳآ .

هﺪﯿﮑﭼ

(2)

ﻪﻣﺪﻘﻣ

زﻮﻠﯾژﺮﭙــﺳآ ﻂــﺳﻮﺗ ﻪــﮐ ﺖــﺳا ﯽﭼرﺎــﻗ يرﺎــﻤﯿﺑ ﺲﯾ

ﻪﻧﻮﮔ يﺎﻫ دﺎﺠﯾا سﻮﻠﯾژﺮﭙﺳآ ﺲﻨﺟ ﻒﻠﺘﺨﻣ ﯽـﻣ

دﻮـﺷ . ﻦـﯾا

چرﺎــﻗ ﺎــﻫ هدر ﺎﺗﻮﮑﯾﺎﻣﻮﮑــﺳآ ﻪﺧﺎــﺷ رد

Eurotiomycetes

ﻪـﺳﺎﻣﻮﮐﻮﮑﯾﺮﺗ هداﻮﻧﺎﺧ آ

و سﻮﻠﯾژﺮﭙـﺳآ ﺲﻨـﺟ ﺪـﻧراد راﺮـﻗ

) 2،

1 .(

دوﺪـﺣ زا ﻞﮑـﺸﺘﻣ سﻮﻠﯾژﺮﭙـﺳآ ﺲﻨﺟ 180

ﻪـﻧﻮﮔ

ﻣ ﯽ ﺪﺷﺎﺑ ﻪﮐ نﻮﻨﮐﺎﺗ 30 زا ﻪﻧﻮﮔ ﺖـﻧﻮﻔﻋ يﺎـﻫ ﺎـﻗ اﺪـﺟ ﯽﭼر

هﺪﺷ ﺪﻧا . ﻊﯾﺎﺷ ﻦﯾﺮﺗ سﻮﻠﯾژﺮﭙـﺳآ زا ﻪـﮐ ﯽﯾﺎـﻫ

ﺖـﻧﻮﻔﻋ يﺎـﻫ

هﺪــﺷ اﺪــﺟ ﯽﭼرﺎــﻗ ،سﻮﺗﺎــﮕﯿﻣﻮﻓ سﻮﻠﯾژﺮﭙــﺳآ ﻞﻣﺎــﺷ ﺪــﻧا

سﻮﻠﯾژﺮﭙــﺳآ ،ﺮﺠﯾﺎــﻧ سﻮﻠﯾژﺮﭙــﺳآ ،سووﻼــﻓ سﻮﻠﯾژﺮﭙــﺳآ سﻮﺋﺮﺗ و ﺲﻧﻻوﺪﯿﻧ سﻮﻠﯾژﺮﭙﺳآ ﯽﻣ

ﺪﻨﺷﺎﺑ ) 4،

3 .(

،ﻦﯾا ﺮﺑ هوﻼﻋ ﻪﻧﻮﮔ

يﺎـﻫ سﻮﻠﯾژﺮﭙـﺳآ هﮋـﯾو ﻂﯾاﺮـﺷ رد

ﻦﯿـــﺴﮐﻮﺗﻮﮑﯾﺎﻣ ﺨﻣ يﺎـــﻫ

،ﻦﯿـــﺴﮐﻮﺗﻼﻓآ ﻞﻣﺎـــﺷ ﻒـــﻠﺘ

ﻦﯿﻟﻮﺗﺎﭘ ،ﻦﯿﺴﮐﻮﺗاﺮﮐا ،ﻦﯿﺴﮐﻮﺗﻮﯾﻼﮔ ﻦﯿﻨﯾﺮﺘﯿﺳ و

ار ﺪـﯿﻟﻮﺗ

ﯽﻣ ﺪﻨﻨﮐ )6

،5 .(

ﮏـﯾژﻮﻟﻮﯿﺑ و ﯽﮑـﺷﺰﭘ ﺖـﯿﻤﻫا ﻪـﺑ ﻪﺟﻮﺗ ﺎﺑ

سﻮﻠﯾژﺮﭙـﺳآ ﻦﯿــﻨﭽﻤﻫ و ﺎـﻫ

يﺎـﻫﻮﮕﻟا ﯽــﯾوراد ﺖﯿـﺳﺎﺴﺣ

رد توﺎﻔﺘﻣ ﻪﻧﻮﮔ

يﺎﻫ ﯽﯾﺎﺳﺎﻨﺷ ،ﻒﻠﺘﺨﻣ ﻖﯿﻗد

ﻦﯾا ﻪـﻧﻮﮔ ﺎـﻫ

ﯽﻣ ﺪﻧاﻮﺗ ﺪـﻨﻤﻓﺪﻫ نﺎﻣرد رد ـﺣ ﺲﯾزﻮﻠﯾژﺮﭙـﺳآ

ﺰﺋﺎ ﺖـﯿﻤﻫا

ﺪﺷﺎﺑ ) 8،

7 .(

ﯽﯾﺎﺳﺎﻨﺷ ﻪﻧﻮﮔ

يﺎﻫ ﺎـﺑ سﻮﻠﯾژﺮﭙـﺳآ شور

يﺎـﻫ و ﺞـﯾار

ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ﯽﺘﻨﺳ توﺎﻔﺗ

يﺎﻫ رﺎﯿـﺴﺑ ﯽـﺋﺰﺟ ﻒـﯾﺮﻇ و ﺎـﻬﻧآ

يدﺎـﯾز ترﺎﻬﻣ و ﻪﺑﺮﺠﺗ مﺰﻠﺘﺴﻣ ،هدﻮﺑ ﺮﯿﮔ ﺖﻗو ﯽـﻣ

ﺪـﺷﺎﺑ .

ﯽﮔﮋﯾو يﺎﻫ ﮏﯾژﻮﻟﻮﻓرﻮﻣ چرﺎﻗ

ﺎﻫ ﺖﺤﺗ ﺮﯿﺛﺄﺗ ﯽﻄﯿﺤﻣ ﻂﯾاﺮﺷ

ﺮـﯿﯿﻐﺗ شﻮﺨﺘـﺳد ﺖـﺳا ﻦﮑﻤﻣ ﺖﺑﻮﻃر و ﺎﻣد ﺮﯿﻈﻧ ﺪﻧﻮـﺷ

) 9 .(

رد لﺎﺳ يﺎﻫ هﺮﯿﺠﻧز ﺶﻨﮐاو يژﻮﻟﻮﻨﮑﺗ دورو ﺎﺑ ﺮﯿﺧا يا

زاﺮﻤﯿﻠﭘ ) (PCR

زا ﯽﺻﺎﺧ تﺎﻌﻄﻗ فﻼﺘﺧا و ﻪﺑﺎﺸﺗ ﯽﺳرﺮﺑ و

لﻮــﮑﻟﻮﻣ ﻞــﺜﻣDNA

)ITS

10 (،

Topoisomerease II

) 11 ( ،

Chitin Synthase

) 12 (،

Calmodulin Gene

) 13 ( ،

Mitochondrial Cytochrome B

) 14 ( و

Beta Tubulin

)Gene

15 ( و زا هدﺎﻔﺘﺳا ﻦﯿﻨﭽﻤﻫ ﮏـﯿﻨﮑﺗ

ﯽﯾﺎـﻫ ﻪـﯾﺎﭘ ﺮـﺑ

شور يﺎـــﻫ ﯽﻟﻮـــﮑﻟﻮﻣ ﻪـــﻠﻤﺟ زا

)RFLP Restriction

Fragment Length Polymorphism

(،

Nested PCR

و ﯽﻟاﻮﺗ ﻦﯿﯿﻌﺗ

نژ يﺎﻫ ،ﯽﺻﺎﺼﺘﺧا ﺖﯿﻘﻓﻮﻣ

يﺎﻫ يﺮﯿﮕﻤـﺸﭼ

ﻖـﯿﻗد ﯽﯾﺎﺳﺎﻨﺷ و ﺺﯿﺨﺸﺗ رد ﺢﻄـﺳ رد ﻢـﺴﯿﻧﺎﮔراوﺮﮑﯿﻣ

ﺖﺳا هﺪﺷ ﻞﺻﺎﺣ ﻪﻧﻮﮔ )

-19 16 .(

ا هدﺎﻔﺘﺳا ﯽـﺳرﺮﺑ رد ﺎﻫﺪـﯿﺗﻮﺌﻠﮐﻮﻧ ﯽﻟاﻮﺗ ﻦﯿﯿﻌﺗ شور ز

نورد عﻮــﻨﺗ ﻪــﻧﻮﮔ

يا ﻦﯿــﺑ و ﻪــﻧﻮﮔ يا رد سﻮﻠﯾژﺮﭙــﺳآ ﺎــﻫ

ﺖﺳا لواﺪﺘﻣ .

ﻦﯾا ﮏـﯿﻨﮑﺗ ياﺮـﺑ شور ﻦﯾﺮـﺘﻬﺑ ﯽﯾﺎـﺳﺎﻨﺷ

هدﻮﺑ ﻢﺴﯿﻧﺎﮔرا رد و

يرﺎﯿﺴﺑ زا تﺎﻌﻟﺎﻄﻣ ﻪﺑ ناﻮﻨﻋ دراﺪﻧﺎﺘـﺳا

رﺎﮑﺑ ﻪﻧﻮﮔ ﻦﯿﯿﻌﺗ ﯽﯾﻼﻃ ﯽﻣ

دور ) 10 .(

نﺎﺸﻧ ﻪﯿﻟوا تﺎﻌﻟﺎﻄﻣ

ﯽﻣ ﺪﻫد نژ ﻪﮐ هﺪﻨﻨﮐ ﺪﮐ ي

ﯽﻨﯿﺌﺗوﺮﭘ ﻦﯿﻟﻮﺑﻮﺗﺎﺘﺑ ﻪﺑ مﻮﺳﻮﻣ

قاﺮـﺘﻓا ياﺮـﺑ ﯽﻓﺎﮐ عﻮﻨﺗ و يراﺪﯾﺎﭘ زا ﻪـﻧﻮﮔ

يﺎـﻫ ﻒـﻠﺘﺨﻣ

ﻪﻠﻤﺟ زا ﯽﭼرﺎﻗ سﻮﻠﯾژﺮﭙﺳآ

ﺎﻫ ﺖﺳا رادرﻮﺧﺮﺑ )

20 ( . فﺪـﻫ

مﺎــﺠﻧا زا ﯽﯾﺎــﺳﺎﻨﺷ ،ﺮــﺿﺎﺣ ﻪــﻌﻟﺎﻄﻣ

ﻪــﻟوﺰﯾا يﺎــﻫ ﯽﻨﯿﻟﺎــﺑ

ﺎﺑ سﻮﻠﯾژﺮﭙﺳآ شور

ﻦﯿﻟﻮﺑﻮﺗ ﺎﺘﺑ نژ ﯽﻟاﻮﺗ ﻦﯿﯿﻌﺗ ﺎﺑ ﻪﮐ هدﻮﺑ

ﻦﯾا شور ﯽﻣ ﻮﺗ نا ﺎﺗ 99

% ﻪﻧﻮﮔ يﺎﻫ ار سﻮﻠﯾژﺮﭙـﺳآ نژﻮﺗﺎﭘ

زا ﺮﮕﯾﺪﮑﯾ داد ﺺﯿﺨﺸﺗ .

شور و داﻮﻣ ﺎﻫ

ﻪﻌﻟﺎﻄﻣ درﻮﻣ ﻪﻌﻣﺎﺟ ﻌﻟﺎﻄﻣ ﮏﯾ ﻖﯿﻘﺤﺗ ﻦﯾا ﮥ

و هدﻮﺑ ﯽﻔﯿﺻﻮﺗ عﻮﻧ زا ﯽﻌﻄﻘﻣ

ﺖﯾﻮﻫ ﻦﯿﯿﻌﺗ نآ فﺪﻫ ﻪﻟوﺰﯾا

يﺎﻫ ﻪﻧﻮﮔ ﺪﺣ رد سﻮﻠﯾژﺮﭙﺳآ

ﻪﺴﯾﺎﻘﻣ زا هدﺎﻔﺘﺳا ﺎﺑ ﯽﻟاﻮـﺗ

يﺎـﻫ نﺎـﻤﻫ ﺎـﺑ ﻦﯿﻟﻮﺑﻮﺗﺎـﺘﺑ نژ

ﯽﻟاﻮﺗ ﺎﻫ رد ﻤﻧ ﻪﻧﻮ يﺎﻫ دراﺪﻧﺎﺘﺳا ﯽﻣ

ﺪﺷﺎﺑ .

يزﺎﺳاﺪﺟ ﻪﻧﻮﻤﻧ

ﺎﻫ

ﻪﻧﻮﻤﻧ ﺎﻫ لﻼﺧ رد لﺎﺳ

يﺎﻫ 1395 - 1394 زا هﺎﮕﺸﯾﺎﻣزآ

ﺎﻔﺷ ) نﺎﻬﻔﺻا ﺮﻬﺷ ﯽﻨﯿﻟﺎﺑ ﯽﺳﺎﻨﺷ چرﺎﻗ ﺮﺘﻧﺎﺳ (

يروآ ﻊـﻤﺟ

و رﺎﮔآ ﺲﮐاد ﮏﭘﺎﭼ ﯽﻗاﺮﺘﻓا ﻂﯿﺤﻣ يور ﺮﺑ )

Czapekdox

(agar

رﺎــﮔآ ﺖﮐﺮﺘــﺴﮐا ﺖــﻟﺎﻣ و )

ﻪــﺑ رﻮــﻈﻨﻣ ﺶﯾاﺰــﻓا

يﺪــﯿﻧﻮﮐ چرﺎــﻗ ﯽــﯾاز

( د ﺖــﺸﮐ ﺪــﺷ هدا

. تﺎﯿــﺻﻮﺼﺧ

ﯽـﻨﻠﮐ ﺖـﺸﭘ و ﺢﻄﺳ ﮓﻧر ،ﺪﺷر ناﺰﯿﻣ ﺪﻨﻧﺎﻣ ﯽﭘﻮﮑﺳوﺮﮐﺎﻣ ﯽـﻨﻠﮐ ﯽﻓاﺮﮔﻮﭘﻮﺗ و ـﺻﻮﺼﺧ و

ﯿ تﺎ ﻪـﻠﻤﺟ زا ﯽﭘﻮﮑـﺳوﺮﮑﯿﻣ

هزاﺪﻧا و ﻞﮑﺷ ماﺪﻧا يﺎﻫ ،لﻮـﮑﯾزو ،رﻮﻓﻮﯾﺪـﯿﻧﻮﮐ ﻞﺜﻣ ﯽﺸﯾاز

ﺪﯾﻻﺎﯿﻓ و ﺎﯾﺪﯿﻧﻮﮐ ﺖﻓﺮﮔ راﺮﻗ ﯽﺳرﺮﺑ درﻮﻣ .

سﺎﺳا ﺮﺑ توﺎﻔﺗ

(3)

ﯽﭘﻮﮑـﺳوﺮﮐﺎﻣ يژﻮـﻟﻮﻓرﻮﻣ رد ﻪﺑﺎﺸﺗ ﺎﯾ و ﮑﯿﻣ و

،ﯽﭘﻮﮑـﺳوﺮ

ﯽﻨﻠﮐ هوﺮﮔ ﺎﻫ ﺪﻧﺪﺷ يﺪﻨﺑ )

2 ( . ﺪﻌﺑ ﻪﻠﺣﺮﻣ رد

، ﻪﻧﻮﻤﻧ ﺎﻫ ياﺮﺑ

ﺪﯿﯾﺄﺗ ﻪـﺘﻓﺎﯾ يﺎـﻫ ﮏـﯿﭙﯿﺗﻮﻨﻓ

، ﻪﺑﺎـﺸﺗ سﺎـﺳا ﺮـﺑ -100

99

ﺪﺻرد ﻦﯿﯿﻌﺗ ﯽﻟاﻮﺗ ﺎﻫ ﺎﺑ ﻪـﻧﻮﻤﻧ يﺎـﻫ رد دﻮـﺟﻮﻣ دراﺪﻧﺎﺘـﺳا

هﺎﮕﯾﺎﭘ هداد يا ﯽﯾﺎﺳﺎﻨﺷNCBI

ﺪﻧﺪﺷ .

ﯽﻟﻮﮑﻟﻮﻣ ﯽﯾﺎﺳﺎﻨﺷ جاﺮﺨﺘﺳا

DNA

جاﺮﺨﺘﺳا ﺖﻬﺟ شور زاDNA

رﺪﻨﯾﺮﮔ مﺮـﻓوﺮﻠﮐ ﻞـﻨﻓ-

هدﺎﻔﺘﺳا ﺪـﺷ ) 21 ( . ﻪـﺑ رﻮـﻃ ندﺮـﮐ دﺮـﺧ زا ﺲـﭘ ﻪـﺻﻼﺧ

،ﯽﻗﺮﺑ بﺎﯿﺳآ ﺎﺑ ﺰﯿﻟ ﺮﻓﺎﺑ رد مﻮﯿﻠﺴﯿﻣ تﺎﻌﻄﻗ ﻪـﺑ ﯽﯾور ﻊﯾﺎﻣ

هﺪﺷ ﻞﻘﺘﻨﻣ ﺪﯾﺪﺟ ﻪﻟﻮﻟ و

ﻢﺠﺣ ﻢﻫ ناﺰﯿﻣ ﻪﺑ

، مﺮﻓوﺮﻠﮐ ﻞﻨﻓ

هدوﺰﻓا ﺮﺘﻧﺎﺳ و ﯾ ژﻮﻔ ﺪﺷ . ﺲﭙﺳ ﺪـﯾﺪﺟ ﻪـﻟﻮﻟ ﻪـﺑ ﯽﯾور ﻊﯾﺎﻣ

ﻞﻘﺘﻨﻣ و زا ﻢـﺠﺣ ﺮـﺑاﺮﺑ ود ناﺰـﯿﻣ ﻪـﺑ -2

ﻧﺎـﭘوﺮﭘ ﻮ ل و 1/

3

هدوﺰﻓا ﻪﻟﻮﻟ ﻪﺑ ﻢﯾﺪﺳ تﺎﺘﺳا زا ﻢﺠﺣ و

يﺎﻣد رد -23

ﻪﺟرد

ﯽﺘﻧﺎﺳ داﺮﮔ ﻪـﻘﯿﻗد ﺖـﺴﯿﺑ تﺪﻣ ﻪﺑ رﺰﯾﺮﻓ ﺪـﺷ ﯽﻫدﺎﻣﺮـﺳ

.

ﻪـــﻘﯿﻗد هد ﺲﭙـــﺳ بﻮـــﺳر

يﺮـــﯿﮔ ـــﺑ ژﻮـــﯿﻓوﺮﮑﯿﻣ رد ﺎ

ﺖﻋﺮﺳ 12000g

مﺎﺠﻧا ﺪﺷ . رد هﺪﺷ ﻞﯿﮑﺸﺗ بﻮﺳر يﺎﻬﺘﻧا

دﻮﺑ هﺪﻫﺎﺸﻣ ﻞﺑﺎﻗ ﻪﻟﻮﻟ .

رﺎـﺒﮑﯾ ،ﻞـﮑﻟا و ﮏـﻤﻧ فﺬـﺣ ياﺮـﺑ

ا ﺎﺑ ﻮﺸﺘﺴﺷ ﻧﺎﺗ

ﻮ ل 70 ا مﺎﺠﻧ ﺪﺷ ندوﺰـﻓا ﺎﺑ ﯽﯾﺎﻬﺘﻧا بﻮﺳر و

20 يﺎـﻣد رد ﺮـﯿﻄﻘﺗ رﺎـﺑ ود ﺮـﻄﻘﻣ بآ زا ﺮﺘﯿﻟوﺮﮑﯿﻣ 20

-

ﻪﺟرد ﯽﺘﻧﺎﺳ داﺮﮔ ﺪﺷ هﺮﯿﺧذ .

وPCR

ﻮﻘﺗ ﯾ ﺖ ﻦﯿﻟﻮﺑﻮﺗﺎﺘﺑ نژ

رد ﻦﯿﻟﻮﺑﻮﺗﺎــﺘﺑ نژ ﺖــﯾﻮﻘﺗ ﺖــﻬﺟ يﺎــﻫﺮﻤﯾاﺮﭘ زاPCR

لﺎﺳرﻮﯿﻧﻮﯾ لوﺪﺟ

1 ﺪﺷ هدﺎﻔﺘﺳا )

22 (.

لوﺪﺟ 1 . يﺎﻫﺮﻤﯾاﺮﭘ هدﺎﻔﺘﺳا درﻮﻣ

ﺖﻬﺟ ﻦﯿﻟﻮﺑﻮﺗﺎﺘﺑ نژ ﺖﯾﻮﻘﺗ

نژ ﺮﻤﯾاﺮﭘ

Bt2α 5' GGTAACCAAATCGGTGCTGCTTTC 3'

Bt2b 5' ACCCTCAGTGTAGTGACCCTTGGC 3'

مﺎــﺠﻧا ياﺮــﺑ راﺪــﻘﻣPCR

5 زا ﺮــﺘﯿﻟوﺮﮑﯿﻣ يDNA

هﺪﺷ جاﺮﺨﺘﺳا ،

25 ﭘﯿ لﻮﻣﻮﮑ ماﺪﮐ ﺮﻫ زا اﺮﭘ زا

ﯾ ﺎـﻫﺮﻤ

، 400

ﻣﯿ وﺮﮑ طﻮﻠﺨﻣ رﻻﻮﻣ

،dNTP

5/

2 ﺮﻓﺎﺑ ﺮﺘﯿﻟوﺮﮑﯿﻣ

،PCR

5/

1

ﯽﻠﯿﻣ رﻻﻮﻣ روﺮﻠﮐ

،ﻢﯾﺰﯿﻨﻣ 5/

2 ﮏـﺗ ﻢﯾﺰـﻧآ ﺪﺣاو زاﺮـﻤﯿﻠﭘ

و

هﺰﯿﻧﻮﯾد ﺮﻄﻘﻣ بآ مزﻻ راﺪﻘﻣ ﻢﺠﺣ ﻪﺑ نﺪﯿﺳر ﺎﺗ ﻞﯾﺮﺘﺳا ي

ﯽﯾﺎﻬﻧ 25 ـﺳ لﺎـﻣﺮﺗ هﺎﮕﺘـﺳد رد و هدوﺰـﻓا ﺮﺘﯿﻟوﺮﮑﯿﻣ ﺎ

ﺮﻠﮑﯾ

ﺪﺷ هداد راﺮﻗ .

ﻪﻣﺎﻧﺮﺑ ﯽﺗراﺮﺣ ي ﻪﺑ

ترﻮﺻ 95 تﺪﻣ ﻪﺑ ﻪﺟرد

5 ،ﻪﻘﯿﻗد 35 ﻞﮑﯿـﺳ 94 تﺪـﻣ ﻪـﺑ ﻪـﺟرد 45

،ﻪـﯿﻧﺎﺛ 55

ﻪﺑ ﻪﺟرد تﺪﻣ 45 و ﻪﯿﻧﺎﺛ 72 تﺪـﻣ ﻪـﺑ ﻪﺟرد 1

و ﻪـﻘﯿﻗد

ﻞﮑﯿﺳ ﮏﯾ 72

تﺪﻣ ﻪﺑ ﻪﺟرد 6

ﻢﯿﻈﻨﺗ ﻪﻘﯿﻗد ﺪﺷ

.

ﺖﻬﺟ ﺖﯾؤر ﻪﺠﯿﺘﻧ ،ﺶﻨﮐاو ي 7

لﻮـﺼﺤﻣ زا ﺮـﺘﯿﻟوﺮﮑﯿﻣ

ﺎﺑPCR

3 لژ يور و طﻮـﻠﺨﻣ يراﺬـﮔرﺎﺑ ﺮﻓﺎـﺑ زا ﺮـﺘﯿﻟوﺮﮑﯿﻣ

زرﺎﮔآ 5/

1 ﺪـﺻرد ﺮﻓﺎـﺑ يوﺎـﺣ ﮏـﻧﺎﺗ نورد و )TBE

ﻞﻣﺎـﺷ

،EDTA Boric Acid

و (Tris

ﺮﺘﮑﻟا ﺪــﺷ زرﻮــﻓو .

لژ ﺎــﻫ ﻪــﺑ

يرادﺮﺒﺴﮑﻋ ﺎﻫﺪﻧﺎﺑ زا و ﻞﻘﺘﻨﻣ رﻮﺗﺎﻨﯿﻣﻮﻟ ﺲﻧاﺮﺗ هﺎﮕﺘﺳد ﺪﺷ

.

ﯽﻟاﻮﺗ ﻦﯿﯿﻌﺗ مﺎــﺠﻧا زا ﺪــﻌﺑ ﻪــﯿﺣﺎﻧ ﺮــﯿﺜﮑﺗ وPCR

،ﻦﯿﻟﻮﺑﻮﺗﺎــﺘﺑ ي

هﺪـﻣآ ﺖـﺳد ﻪـﺑ لﻮﺼﺤﻣ ﺺﯿـﻠﺨﺗ ﺖـﯿﮐ زا هدﺎﻔﺘـﺳا ﺎـﺑ

لﻮـﺼﺤﻣ )PCR

AccuPrep PCR Purification Kit

( ﻪـﺑ

ﯽﻧﻮﺘﺳ شور ﺺﯿﻠﺨﺗ

ﻌﺗ و ﯿﯿ ﻦ ﯽﻟاﻮﺗ ﻪﯾاﺪﺟ ﻫﺎ ﻪـﺑ ترﻮـﺻ

مﺎﺠﻧا ﻪﻓﺮﻃ ﮏﯾ ﺪﺷ

. ﮏـﺗ ﮏـﺗ يﺪـﯿﺗﻮﺌﻠﮐﻮﻧ مﺎـﺧ ﯽﻟاﻮﺗ

ﻪﻧﻮﻤﻧ ﺎﻫ مﺮﻧ ﺎﺑ راﺰﻓا

MEGA 4

ﺐﯿﺗﺮﺗ ﻦﯾﺪﺑ و ﺪﺷ ﺶﯾاﺮﯾو

ﯽﻟاﻮﺗ ﻦﯿﯿﻌﺗ ﺮﻫ ﻦﯿﻟﻮﺑﻮﺗﺎﺘﺑ ﯽﯾﺎﻬﻧ

ﻪﯾاﺪﺟ ﺪـﻣآ ﺖـﺳد ﻪـﺑ .

ﻦﯿﯿﻌﺗ ﯽﻟاﻮﺗ ﺎﻫ مﺮﻧ زا هدﺎﻔﺘﺳا ﺎﺑ راﺰـﻓا

BLAST

ﯽﻟاﻮـﺗ ﺎـﺑ

نژ ﮏـﻧﺎﺑ رد ﻪﺑﺎﺸﻣ )

GenBank

( ﻟﺎـﻧآ و ﻪـﺴﯾﺎﻘﻣ درﻮـﻣ ﺰﯿ

ﺘﻓﺮﮔ راﺮﻗ ﺪﻨ . ﯽﯾﺎﺳﺎﻨﺷ ﻪﯾاﺪﺟ

ﺎﻫ ي ﺮﻈﻧ درﻮﻣ زا هدﺎﻔﺘﺳا ﺎﺑ

ﻻﺎـﺑ ﯽﻟاﻮﺗ ﻪﺑﺎﺸﺗ ي

100 - 99 ﺪـﺻرد ﺎﭘ رد ﯾ هﺎـﮕ هداد يﺎـﻫ

ﺪﺷ مﺎﺠﻧا NCBI

) 15 .(

ﻪﺘﻓﺎﯾ ﺎﻫ

زا 465 ،هﺎﮕـﺸﯾﺎﻣزآ ﻪـﺑ هﺪـﻨﻨﮐ ﻪـﻌﺟاﺮﻣ ﺮﻔﻧ 39

رﺎـﻤﯿﺑ

) 4/

8

% ( ﻮﻠﯾژﺮﭙﺳآ ﻪﺑ ﻼﺘﺒﻣ ﯾز

ﺪﻧﺪﺷ ﯽﯾﺎﺳﺎﻨﺷ ﺲ .

ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ

شور يﺎﻫ ،ﮏﯿﭙﯿﺗﻮﻨﻓ اﺪﺟ

ﻪﯾ ﺎﻫ ﮏـﯾژﻮﻟﻮﻓرﻮﻣ هوﺮـﮔ ﺪﻨﭼ ﻪﺑ

ﺲﭙﺳ ،هﺪﺷ ﻢﯿﺴﻘﺗ ﻪﯾاﺪﺟ

ﺎﻫ شور ﺎﺑ ﻪـﯿﺣﺎﻧ ﯽﻟاﻮـﺗ ﻦﯿـﯿﻌﺗ

ﻧژ ﯽ ﻦﯿﻟﻮﺑﻮﺗ ﺎﺘﺑ ،

ﺪﯿﯾﺄﺗ ﺪﻧﺪﺷ . ﺶﻨﮐاو رد هﺮﯿﺠﻧز

يا زاﺮﻤﯿﻠﭘ ،

ﻪﯿﻠﮐ ﻦﯿﻟﻮﺑﻮﺗ ﺎﺘﺑ ﻪﯿﺣﺎﻧ ﻪﻧﻮﻤﻧ

ﺎﻫ ﺪﻧﺪـﺷ زرﻮﻓوﺮﺘﮑﻟا و ﺖﯾﻮﻘﺗ

ﯽﺒﯾﺮﻘﺗ هزاﺪﻧا ﻪﺑ ﺪﻧﺎﺑ ﮏﯾ ﻪﮐ 570

- 540 زﺎﺑ ﺖﻔﺟ

، هﺪﻫﺎﺸﻣ

ﺪﯾدﺮﮔ ) ﺮﯾﻮﺼﺗ 1 (.

(4)

ﺮﯾﻮﺼﺗ 1 . لﻮﺼﺤﻣ زرﻮﻓوﺮﺘﮑﻟا ﺣﺎﻧPCR

ﯿ ﻪ ﺎﺘﺑ ﺪﻨﭼ ﻦﯿﻟﻮﺑﻮﺗ

سﻮﻠﯾژﺮﭙﺳآ ﻪﻧﻮﻤﻧ .

ﻒﯾدر 1 و 2 : ﻒﯾدر ،سووﻼﻓ سﻮﻠﯾژﺮﭙﺳآ 3

: ﻒﯾدر ،هﺰﯾروا سﻮﻠﯾژﺮﭙﺳآ 4

: سﻮﻠﯾژﺮﭙﺳآ

ﻒﯾدر ،ﺲﯿﺴﻨﺠﻨﯿﺑﻮﺗ :N

ﻒﯾدر ،ﯽﻔﻨﻣ لﺮﺘﻨﮐ :M

ﺮﮐرﺎﻣ 100 يزﺎﺑ ﺖﻔﺟ

ﯽﮔﮋﯾو رﻮﻓﻮﯾﺪﯿﻧﻮﮐ ﺮﻄﻗ و لﻮﻃ ﺪﻨﻧﺎﻣ ﯽﭘﻮﮑﺳوﺮﮑﯿﻣ يﺎﻫ ،

لﻮﮑﯾزو هزاﺪﻧا و ﻞﮑﺷ ﯽـﻔﯾدر ﮏﯾ ،ﺎﻫﺪﯿﻟﺎﯿﻓ هزاﺪﻧا و ﻞﮑﺷ ،ﺎﻫ

يﺪـﯿﻧﻮﮐ هزاﺪـﻧا و ﻞﮑﺷ و ﺎﻫﺪﯿﻟﺎﯿﻓ ندﻮﺑ ﯽﻔﯾدر ود ﺎﯾ رد ﺎـﻫ

ﺮﯾﻮﺼﺗ 2 ﯽﻣ هﺪﻫﺎﺸﻣ دﻮﺷ

.

ﻪﯾاﺪﺟ ﻪﮐA

ﯽﻟاﻮﺗ ﻦﯿﯿﻌﺗ و يژﻮﻟﻮﻓرﻮﻣ شور ﺎﺑ ﻪﺑ

ناﻮـﻨﻋ

ﺪﺷ ﯽﯾﺎﺳﺎﻨﺷ سﻮﺋﺮﺗ سﻮﻠﯾژﺮﭙﺳآ ياراد ،

رﻮﻓﻮﯾﺪـﯿﻧﻮﮐ ﻢﺘـﺧ

هﺪﺷ لﻮﮑﯾزو ﮏﯾ ﻪﺑ ﺮﻘﺗ

ًﺎﺒﯾ دﺮﮔ و هﺪﯿﺸﮐ ﺖﺳا

ﻪﮐ ﯽـﻤﯿﻧ رد ﺎﻤﮕﯾﺮﺘﺳا ﻒﯾدر ود ﺎﯾ ﮏﯾ لﻮﮑﯾزو ﺢﻄﺳ زا

دراد راﺮﻗ ) ﺮﯾﻮﺼﺗ

-2 (.A

ﻪﯾاﺪﺟ )B

سووﻼـﻓ سﻮﻠﯾژﺮﭙـﺳآ (

ياراد رﻮﻓﻮﯾﺪـﯿﻧﻮﮐ

ﻢﯿﺨﺿ هراﻮﯾد ﺎﺑ ﺪﻨﻠﺑ رادرﺎﺧ و

ﺪـﯾﻻﺎﯿﻓ ،يوﺮـﮐ لﻮﮑﯾزو ، يﺎﻫ

ﻪﻔﯾدر ود ﺎﯾ ﮏﯾ دﻮﺑ

) ﺮﯾﻮﺼﺗ -2 ( .B

سﻮﺗﺎـﮕﯿﻣﻮﻓ سﻮﻠﯾژﺮﭙﺳآ

ياراد ﮐ لﻮــﮑﯾزو هﺪﯿــﺸ

ﺮــﻄﺑ و ي ﻞﮑــﺷ ) ﺮﯾﻮــﺼﺗ -2

(،C

ﺮﺠﯾﺎﻧ سﻮﻠﯾژﺮﭙﺳآ ﺎـﺑ

و ﻪـﻔﯾدر ود ﺪـﯾﻻﺎﯿﻓ ،يوﺮـﮐ لﻮـﮑﯾزو

ﮓــﻧر هﺎﯿــﺳ يوﺮــﮐ يﺪــﯿﻧﻮﮐ )

ﺮﯾﻮــﺼﺗ -2 (،D

سﻮﻠﯾژﺮﭙــﺳآ

ﯿﺑﻮﺗ ﺠﻨ و ﻪــﻔﯾدر ود ﺪــﯾﻻﺎﯿﻓ ،يوﺮــﮐ لﻮــﮑﯾزو ياراد ﺲﯿــﺴﻨ

ﮓــﻧر هﺎﯿــﺳ يوﺮــﮐ يﺪــﯿﻧﻮﮐ )

ﺮﯾﻮــﺼﺗ 2 - (E

سﻮﻠﯾژﺮﭙــﺳآ ،

يرﻮﻣاوآ ﺎﺑ يﺪﯿﻧﻮﮐ ﺎﻫ ي ﻪﻌـﺷا ﯽﻧﻮﺘـﺳ و راو ور ﺮـﺑ

ي ﺪـﯾﻻﺎﯿﻓ

) ﺮﯾﻮﺼﺗ -2 (F

و اﺰﯾروا سﻮﻠﯾژﺮﭙﺳآ ﺎـﺑ

ﯽـﺑﻼﮔ ،دﺮـﮔ لﻮـﮑﯾزو

ار لﻮﮑﯾزو ﻞﮐ ﺢﻄﺳ ﺪﯾﻼﯿﻓ ﻪﮐ ﻞﮑﺷ ﯽﻣ

ﺪﻧﺎـﺷﻮﭘ ﯽﯾﺎـﺳﺎﻨﺷ ،

ﺪﻧﺪﺷ ) ﺮﯾﻮﺼﺗ -2 (.G

ﺗ ﺮﯾﻮﺼ 2 . زا يداﺪﻌﺗ ﯽﭘﻮﮑﺳوﺮﮑﯿﻣ هﺮﻈﻨﻣ اﺪﺟ

ﯾ ﻪ ﺎﻫ ي ﯽﯾﺎﺳﺎﻨﺷ يژﻮﻟﻮﻓرﻮﻣ شور و ﻦﯿﻟﻮﺑﻮﺗ ﺎﺘﺑ نژ ﯽﻟاﻮﺗ ﻦﯿﯿﻌﺗ ﻂﺳﻮﺗ ﻪﮐ سﻮﻠﯾژﺮﭙﺳآ

ﮔ ﺪﯾدﺮ . :A ،سﻮﺋﺮﺗ سﻮﻠﯾژﺮﭙﺳآ :B

سووﻼﻓ سﻮﻠﯾژﺮﭙﺳآ ،

:C سﻮﺗﺎﮕﯿﻣﻮﻓ سﻮﻠﯾژﺮﭙﺳآ ،

:D ﺮﺠﯾﺎﻧ سﻮﻠﯾژﺮﭙﺳآ ،

:E ﻨﯿﺑﻮﺗ سﻮﻠﯾژﺮﭙﺳآ ﺠ

ﺲﯿﺴﻨ ، :F يرﻮﻣاوآ سﻮﻠﯾژﺮﭙﺳآ ،

:G اﺰﯾروا سﻮﻠﯾژﺮﭙﺳآ

(5)

ﯽﯾﺎﺳﺎﻨﺷ ﺮﺿﺎﺣ ﻪﻌﻟﺎﻄﻣ رد ﻪﻧﻮﮔ

ﺎـﻫ يژﻮـﻟﻮﻓرﻮﻣ شور ﻪـﺑ

ﺺﯿﺨــﺸﺗ ﻪــﺑ ﺮــﺠﻨﻣ 4

سووﻼــﻓ سﻮﻠﯾژﺮﭙــﺳآ ﻪــﻧﻮﮔ )

77

%(

،

سﻮﺗﺎـﮕﯿﻣﻮﻓ سﻮﻠﯾژﺮﭙﺳآ )

10

%(

ﺮﺠﯾﺎـﻧ سﻮﻠﯾژﺮﭙـﺳآ ، )

10

%(

،

سﻮﺋﺮﺗ سﻮﻠﯾژﺮﭙﺳآ )

2

% ( ﺪﯾدﺮﮔ . ،نارﺎـﻤﯿﺑ ﯽﻨـﺳ ﻦﯿﮕﻧﺎﯿﻣ 21

ﺎﺗ 73 دﻮﺑ لﺎﺳ ) لوﺪﺟ 2 .(

طﻮـﺑﺮﻣ ﯽﻨﯿﻟﺎـﺑ ﻪﻧﻮﻤﻧ ﻪﺳ و ﺖﺴﯿﺑ

ﺎﭘ و ﺖﺳد ﻦﺧﺎﻧ ﻪﺑ )

59 (%

، 5 ﻂﻠﺧ ﻪﻧﻮﻤﻧ )

13 (%

، 5 ﻪﯾر ﻪﻧﻮﻤﻧ

) 13 (%

، 3 ﺪـﻠﺟ ﻪﻧﻮﻤﻧ )

8 (%

،2 شﻮـﮔ ﻪـﻧﻮﻤﻧ )

5 (%

و 1 ﻧ ﻪـﻧﻮﻤ

لازﺎﻧارﺎﭘ ) 2 (%

ﺪﻧدﻮﺑ . ﻦﯿـﯿﻌﺗ و ﻦﯿﻟﻮـﺑﻮﺗ ﺎـﺘﺑ نژ ﻪـﯿﺣﺎﻧ ﺖﯾﻮﻘﺗ ﯽﯾﺎـﺳﺎﻨﺷ ﻪـﺑ ﺮـﺠﻨﻣ ﻪﯿﺣﺎﻧ ﻦﯾا ﯽﻟاﻮﺗ

7 سﻮﻠﯾژﺮﭙـﺳآ زا ﻪـﻧﻮﮔ

دﺮﮔ ﯾﺪ ﻪﮐ ﻞﻣﺎﺷ ﺐﯿﺗﺮﺗ ﻪﺑ 22

اﺪﺟ ﯾﻪ ﻪـﺑ ناﻮـﻨﻋ سﻮﻠﯾژﺮﭙـﺳآ

سووﻼﻓ ) 56 (%

، 8 آﺰﯾروا سﻮﻠﯾژﺮﭙﺳآ ﻪﯾاﺪﺟ )

20 (%

، 4 اﺪﺟ ﯾﻪ

ﻪـــﺑ ناﻮـــﻨﻋ سﻮﺗﺎـــﮕﯿﻣﻮﻓ سﻮﻠﯾژﺮﭙـــﺳآ )

10 (%

، 2 ﻪـــﯾاﺪﺟ

ﻨﯿﺑﻮﺗ سﻮﻠﯾژﺮﭙﺳآ ﺠ

ﺲﯿﺴﻨ ) 5 (%

، 1 اﺪﺟ ﯾﻪ ﺮﺠﯾﺎـﻧ سﻮﻠﯾژﺮﭙﺳآ

) 2 (%

، 1 اﺪﺟ ـــﯾ ﻪ سﻮـــﺋﺮﺗ سﻮﻠﯾژﺮﭙـــﺳآ )

2 (%

و 1 ﻪـــﯾاﺪﺟ

يرﻮﻣاوآ سﻮﻠﯾژﺮﭙﺳآ )

2 (%

ﺪﻧﺪﺷ ﯽﯾﺎﺳﺎﻨﺷ .

59

% نز نارﺎـﻤﯿﺑ

و 41 % ﺪﻧدﻮﺑ دﺮﻣ )

لوﺪﺟ 2 (.

لوﺪﺟ 2 . ﻣ شور ود ﺎﺑ هﺪﺷ اﺪﺟ يﺎﻫ سﻮﻠﯾژﺮﭙﺳآ ﺺﯿﺨﺸﺗ و نارﺎﻤﯿﺑ تﺎﺼﺨﺸﻣ رﻮ

ﮏﯾژﻮﻟﻮﻓ ﻟﻮﮑﻟﻮﻣ و ﯽ

ﻪﻧﻮﻤﻧ ﻪﻌﯾﺎﺿ ﻞﺤﻣ

ﻦﺳ ﺲﻨﺟ

ﯽﻟﻮﮑﻟﻮﻣ شور ﺎﺑ ﻪﻧﻮﮔ ﺺﯿﺨﺸﺗ يژﻮﻟﻮﻓرﻮﻣ شور ﺎﺑ ﻪﻧﻮﮔ ﺺﯿﺨﺸﺗ

1 ﺖﺳد ﻦﺧﺎﻧ 21

نز سﻮﺋﺮﺗ سﻮﻠﯾژﺮﭙﺳآ

سﻮﺋﺮﺗ سﻮﻠﯾژﺮﭙﺳآ

2 ﺶﻧﺮﺑ

72 نز

سﻮﺗﺎﮕﯿﻣﻮﻓ سﻮﻠﯾژﺮﭙﺳآ سﻮﺗﺎﮕﯿﻣﻮﻓ سﻮﻠﯾژﺮﭙﺳآ

3 ﺎﭘ ﻦﺧﺎﻧ

32 دﺮﻣ

سووﻼﻓ سﻮﻠﯾژﺮﭙﺳآ سووﻼﻓ سﻮﻠﯾژﺮﭙﺳآ

4 لازﺎﻧارﺎﭘ

53 دﺮﻣ

55 نز

51 نز

7 ﻪﯾر

67 دﺮﻣ

59 دﺮﻣ

9 ﺖﺳﻮﭘ

54 نز

يرﻮﻣاوآ سﻮﻠﯾژﺮﭙﺳآ ﺮﺠﯾﺎﻧ سﻮﻠﯾژﺮﭙﺳآ

10 ﻂﻠﺧ

42 دﺮﻣ

اﺰﺑروا سﻮﻠﯾژﺮﭙﺳآ سووﻼﻓ سﻮﻠﯾژﺮﭙﺳآ

31 نز

49 نز

30 نز

14 ﭘ ﻦﺧﺎﻧ

ﺎ 48

نز سووﻼﻓ سﻮﻠﯾژﺮﭙﺳآ

سووﻼﻓ سﻮﻠﯾژﺮﭙﺳآ

63 نز

16 ﻂﻠﺧ

50 دﺮﻣ

17 ﻂﻠﺧ

71 دﺮﻣ

18 66 BAL

نز سﻮﺗﺎﮕﯿﻣﻮﻓ سﻮﻠﯾژﺮﭙﺳآ

سﻮﺗﺎﮕﯿﻣﻮﻓ سﻮﻠﯾژﺮﭙﺳآ

19 ﭘ ﻦﺧﺎﻧ

ﺎ 71

دﺮﻣ سووﻼﻓ سﻮﻠﯾژﺮﭙﺳآ

73 نز

22 ﻪﯾر

70 دﺮﻣ

ﺲﯿﺴﻨﺠﻨﯿﺑﻮﺗ سﻮﻠﯾژﺮﭙﺳآ ﺮﺠﯾﺎﻧ سﻮﻠﯾژﺮﭙﺳآ

23 ﺖﺳﻮﭘ

70 دﺮﻣ

ﺮﺠﯾﺎﻧ سﻮﻠﯾژﺮﭙﺳآ ﺮﺠﯾﺎﻧ سﻮﻠﯾژﺮﭙﺳآ

24 ﻦﺧﺎﻧ

ﺎﭘ 32

نز اﺰﺑروا سﻮﻠﯾژﺮﭙﺳآ

25 شﻮﮔ

43 دﺮﻣ

26 شﻮﮔ

52 نز

ﺲﯿﺴﻨﺠﻨﯿﺑﻮﺗ سﻮﻠﯾژﺮﭙﺳآ ﺮﺠﯾﺎﻧ سﻮﻠﯾژﺮﭙﺳآ

41 نز

29 دﺮﻣ

29 ﺧﺎﻧ

ﻦ 26

نز اﺰﺑروا سﻮﻠﯾژﺮﭙﺳآ

30 ﺖﺳﻮﭘ

31 نز

31 ﻪﯾر

62 دﺮﻣ

32 ﻦﺧﺎﻧ

44 دﺮﻣ

47 نز

34 ﻦﺧﺎﻧ

ﺎﭘ 32

35 ﻂﻠﺧ

22 دﺮﻣ

36 ﻂﻠﺧ

38 نز

57 نز

دﺮﻣ سووﻼﻓ سﻮﻠﯾژﺮﭙﺳآ

39 ﻦﺧﺎﻧ

ﺎﭘ 63

(6)

يﺮﯿﮔ ﻪﺠﯿﺘﻧ و ﺚﺤﺑ

ﺲﻨﺟ ياراد سﻮﻠﯾژﺮﭙﺳآ ﻪﻧﻮﮔ

يﺎﻫ يدﺪﻌﺘﻣ ﯽـﻣ ﺪـﺷﺎﺑ

داﺮﻓا رد ﻪﮐ ﺎﺑ

ﯽﻨﻤﯾا ﻢﺘﺴﯿﺳ ﻒﻌﺿ يرﺎﻤﯿﺑ دﺎﺠﯾا

ﯽﻣ ﺪﻨﻨﮐ .

ﻪﻧﻮﮔ ﺎﻫ ﻞﻣﺎﻋ سﻮﻠﯾژﺮﭙﺳآ ي يرﺎﻤﯿﺑ

يﺎﻫ يﺮﻧﻮـﻤﻟﻮﭘ ﻮﮑﻧوﺮﺑ

،شﻮـــﮔ ﯽﭼرﺎـــﻗ ﺖـــﻧﻮﻔﻋ ،ﯽﭼرﺎـــﻗ ﺖـــﯿﺗاﺮﮐ ،ﮏـــﯾژﺮﻟآ ﺖﯾزﻮﻨﯿﺳ يﺎﻫ ﻦﯿﻨﭽﻤﻫ و لازﺎﻧ ﺖﻧﻮﻔﻋ

يﺎﻫ ﻢﺟﺎـﻬﻣ يﻮـﯾر

ﺪﻨﺘﺴﻫ . ﺺـﻘﻧ ياراد داﺮﻓا رد ﺮﺘﺸﯿﺑ ﯽﭼرﺎﻗ ﻢﺟﺎﻬﻣ يرﺎﻤﯿﺑ

ﺎﯾ ﯽﻨﻤﯾا ﻪﮐ ﯽﻧﺎﺴﮐ ﺖـﺤﺗ

ﺮﯿﺛﺄـﺗ يﺎـﻫوراد اﯾ

ـﺳﺮﭘﺎﺳﻮﻨﻤ ﯿﻮ

هﺪﯾد ﺪﻨﺘﺴﻫ ﯽﻣ

دﻮﺷ . زا يرﺎﯿـﺴﺑ ﻪـﻧﻮﮔ

يﺎـﻫ سﻮﻠﯾژﺮﭙـﺳآ

نژﺮﻟآ ﯽﻣ ﺪﻨﺷﺎﺑ ﻦﯾاﺮﺑﺎﻨﺑ ، ﻢﻬﻣ زا

ﻦﯾﺮﺗ ﻢـﺳآ يرﺎـﻤﯿﺑ ﻞﻣاﻮﻋ

ﺸﻨﻣ ﺎﺑ ءﺎ بﻮﺴﺤﻣ ﯽﺟرﺎﺧ ﯽﻣ

ﺪﻧﻮﺷ مدا ﺪﻨﻧﺎﻣ ﯽﻤﺋﻼﻋ ﺎﺑ ﻪﮐ

هاﺮـﻤﻫ ﺶﻧوﺮـﺑ ﻢﺳﺎﭙﺳا و ﯽـﻣ

ﺪـﺷﺎﺑ ) 23

، 18 .(

سﺎـﺳا ﺮـﺑ

ﺪـﯾﺪﺟ يﺪﻨﺑ ﻢﯿﺴﻘﺗ

، ﻪـﺑ سﻮﻠﯾژﺮﭙـﺳآ ﺲﻨـﺟ هوﺮـﮔ

يﺎـﻫ

يوﺎﺣ دﻮﺧ ﺶﺨﺑ ﺮﻫ ﻪﮐ ﺖﺳا هﺪﺷ ﻢﯿﺴﻘﺗ ﻒﻠﺘﺨﻣ ﻪﻧﻮﮔ

ﺎﻫ

ﻪﺘﯾراو و ﯿﻧ ،ﯽﺗﺎـﮕﯿﻣﻮﻓ ،يوﻼـﻓ ﺪـﻨﻧﺎﻣ ﯽﻔﻠﺘﺨﻣ يﺎﻫ ـﮕ

يﺮ و

ﻏﯿ هﺮ ﯽـﻣ ـﺷﺎﺑ ﻨ ﺪ ) -26 24 .(

شور يﺎـﻫ ياﺮـﺑ ﮏـﯾژﻮﻟﻮﻓرﻮﻣ

ﻦﯿﯿﻌﺗ ﻪﻧﻮﮔ يﺎـﻫ ﻧ ﺮـﺒﺘﻌﻣ و ﯽﻓﺎـﮐ سﻮﻠﯾژﺮﭙـﺳآ ﯽـﻤ

ﺪـﺷﺎﺑ

ﻦﯾاﺮﺑﺎﻨﺑ ﺑ قاﺮﺘﻓا ياﺮﺑ ﻖﯿﻗد شور ﮏﯾ يﺮﯿﮔرﺎﮑ ﻪـﻧﻮﮔ

يﺎـﻫ

سﻮﻠﯾژﺮﭙﺳآ ﺮﻈﻧ ﻪﺑ يروﺮﺿ

ﯽﻣ ﺪﺳر . رد لﺎﺳ يﺎﻫ ﺎـﺑ ﺮـﯿﺧا

هﺮﯿﺠﻧز ﺶﻨﮐاو يژﻮﻟﻮﻨﮑﺗ دورو يا

زاﺮﻤﯿﻠﭘ ) (PCR

ﯽﺳرﺮﺑ و

ﻮﻣ زا ﯽــﺻﺎﺧ تﺎــﻌﻄﻗ فﻼﺘــﺧا و ﻪﺑﺎــﺸﺗ لﻮــﮑﻟ

،DNA

ﭘﯿ ﺖﻓﺮﺸ يﺎﻫ ﻞـﺻﺎﺣ ﻪـﻧﻮﮔ ﺺﯿﺨﺸﺗ ﻪﻨﯿﻣز رد يﺮﯿﮕﻤﺸﭼ

ﺖﺳا هﺪﺷ .

نﺎﺸﻧ تﻻﺎﻘﻣ روﺮﻣ ﯽﻣ

ﺪﻫد ﻪـﮐ ًﺎـﺒﯾﺮﻘﺗ ﯽﻣﺎـﻤﺗ

شور يﺎــﻫ ﻪــﺘﻓر رﺎــﮑﺑ

DNA-based

ﯽﯾﺎــﺳﺎﻨﺷ ياﺮــﺑ ﻪــﮐ

سﻮﻠﯾژﺮﭙﺳآ ﺎﻫ

ﻪﺘﻓر رﺎﮐ ﻪﺑ ﺖﺳا

) ﻪﺑ يﺎﻨﺜﺘﺳا ﯽﻟاﻮـﺗ ﻦﯿﯿﻌﺗ (

زا يدوﺪﻌﻣ داﺪﻌﺗ سﻮﻠﯾژﺮﭙـﺳآ

ﺎـﻫ ﯽﯾﺎـﺳﺎﻨﺷ ار هدﺮـﮐ

ﺪـﻧا .

ﺘﺸﯿﺑ ﻦﯾﺮ ﻪﻧﻮﻤﻧ ﺎﻫ ﻦﺧﺎﻧ ﻪﺑ طﻮﺑﺮﻣ و ﺖﺳد

ﺎﭘ ) 4/

56

%(

دﻮـﺑ

ﻪﮐ ﺖﺷاد ﻖﺑﺎﻄﺗ نارﺎﮑﻤﻫ و ﯽﻫﻮﮑﺷ ﻪﻌﻟﺎﻄﻣ ﺎﺑ عﻮﺿﻮﻣ ﻦﯾا

) 27 .(

زا ،ﯽﻟﻮـﮑﻟﻮﻣ شور ﺎﺑ ﻪﺴﯾﺎﻘﻣ رد 39

ﻪـﯾاﺪﺟ ﻪـﮐ ﻪـﺑ

،ﺪــﯾدﺮﮔ ﯽﯾﺎــﺳﺎﻨﺷ يژﻮــﻟﻮﻓرﻮﻣ شور ﺖــﯾﻮﻫ

11 ﻪــﻧﻮﮔ

ﻪﺑ ترﻮﺻ ﺪﻧﺪﺷ ﯽﯾﺎﺳﺎﻨﺷ ﺖﺳردﺎﻧ .

ﻪﯾاﺪﺟ ﺎﻫ ﯾ ﺮـﺑ ﺐﻠﻏا ﻪﮐ ﯽ

ﯽـﺳﺎﻨﺷ ﺖـﺨﯾر سﺎﺳا ،ﯽﭘﻮﮑـﺳوﺮﮑﯿﻣ و ﯽـﻨﻠﮐ

ﻪـﺑ ناﻮـﻨﻋ ﻪﺑ ﻖﻠﻌﺘﻣ ﻊﻗاو رد ﺪﻧدﻮﺑ هﺪﺷ ﻪﺘﺧﺎﻨﺷ ﺮﺠﯾﺎﻧ سﻮﻠﯾژﺮﭙﺳآ

3

و اﺰﺠﻣ ﻪﻧﻮﮔ ﻏﯿ

ﻞﺑﺎﻗﺮ مﺎـﻧ ﻪـﺑ يژﻮـﻟﻮﻓرﻮﻣ ظﺎـﺤﻟ زا قاﺮﺘﻓا

ﻨﯿﺑﻮﺗ سﻮﻠﯾژﺮﭙـــﺳآ ،ﺮﺠﯾﺎـــﻧ سﻮﻠﯾژﺮﭙـــﺳآ ﺠ

ﺲﯿـــﺴﻨ و

يرﻮﻣاوآ سﻮﻠﯾژﺮﭙﺳآ ﺪﻧدﻮﺑ

ﻪـﻧﻮﮔ ﻪـﺑ ﻖـﻠﻌﺘﻣ ﻪـﺳ ﺮـﻫ ﻪﮐ

سﻮﻠﯾژﺮﭙﺳآ ﺲﻨﺟ زا يﺮﮕﯿﻧ ﯽﻣ

ﺪﺷﺎﺑ ﻦﯾا و شور ﺎـﺑ ﻂـﻘﻓ

ﺰﯾﺎﻤﺗ ﻞﺑﺎﻗ ﯽﻟﻮﮑﻟﻮﻣ ﺖﺳا

. ﻪـﻌﻟﺎﻄﻣ ﻦﯾا رد ﻪﮐ يﺮﮕﯾد ﻪﻧﻮﮔ

اﺰﯾروا سﻮﻠﯾژﺮﭙﺳآ ﺪﺷ اﺪﺟ ﻪـﮐ دﻮﺑ

ﺖﻫﺎﺒـﺷ ياراد رﺎﯿـﺴﺑ

يدﺎﯾز سووﻼﻓ سﻮﻠﯾژﺮﭙﺳآ ﻪﺑ ﯽـﻣ

ﺪـﺷﺎﺑ . ﻪـﻌﻟﺎﻄﻣ ﻦـﯾا رد

ﺪﺻرد ﯽﻧاواﺮﻓ سﻮﻠﯾژﺮﭙﺳآ ﺎﻫ

ﻞﻣﺎﺷ ﺐﯿﺗﺮﺗ ﻪﺑ سﻮﻠﯾژﺮﭙـﺳآ

سووﻼــــﻓ )

4/

56

%(

، اﺰــــﯾروا سﻮﻠﯾژﺮﭙــــﺳآ )

5/

20

%(

،

ــﺳآ سﻮﺗﺎــﮕﯿﻣﻮﻓ سﻮﻠﯾژﺮﭙ )

2/

10

%(

، سﻮــﺋﺮﺗ سﻮﻠﯾژﺮﭙــﺳآ

) 5/

2

% ( يرﻮﻣاوآ سﻮﻠﯾژﺮﭙﺳآ و )

5/

2

% ( ﻪـﮐ دﻮﺑ ﻪﺑﺎـﺸﻣ ﺎـﺑ

نارﺎــﮑﻤﻫ و يﺪــﻨﻫﺮﯿﻣ ﻪــﻌﻟﺎﻄﻣ

، سووﻼــﻓ سﻮﻠﯾژﺮﭙــﺳآ

ﻊﯾﺎﺷ ﻦﯾﺮﺗ دﻮﺑ ﯽﯾﺎﺳﺎﻨﺷ درﻮﻣ ﻪﯾاﺪﺟ )

9 .(

رد ﻪﻌﻟﺎﻄﻣ يا ﻪﮐ

يرﻮﻧ نارﺎﮑﻤﻫ و رﻮﭘ ور ﺮﺑ

ي ﻪﻧﻮﻤﻧ يﺎﻫ مﺎـﺠﻧا زﻮﮑﯾﺎﻣﻮﮑﯿﻧوا

ﺘﺸﯿﺑ ،ﺪﻧداد ﺖﯿﻓوﺮﭘﺎـﺳ ﯽﻧاواﺮﻓ ﻦﯾﺮ

ﺲﻨـﺟ ﻪـﺑ طﻮـﺑﺮﻣ ﺎـﻫ

ﻖﺑﺎﻄﺗ ﺮﺿﺎﺣ ﻪﻌﻟﺎﻄﻣ ﺎﺑ ﻪﮐ دﻮﺑ سووﻼﻓ ﻪﻧﻮﮔ و سﻮﻠﯾژﺮﭙﺳآ ﺖﺷاد ) 28 .(

ﻪﻌﻟﺎﻄﻣ رد رﺎـﻫوا

ﻂـﺳﻮﺘﻣ ﻦـﺳ ،نارﺎـﮑﻤﻫ و

نارﺎﻤﯿﺑ 71 و دﻮﺑ لﺎﺳ نز

ﺎﻫ عﻮﯿﺷ ﺎﺑ 58 % هوﺮـﮔ ﻦﯾﺮﺘﺸﯿﺑ

ﺪﻧدﻮﺑ ﺮﯿﮔرد .

ﻪﻧﻮﮔ يﺎﻫ سﻮﺗﺎـﮕﯿﻣﻮﻓ )

48 (%

ﺮـﺠﯿﻧ و ) 29 (%

ﻦﯾﺮﺘﺸﯿﺑ ﻪـﯾاﺪﺟ

ﺎـﻫ يﺮﻧﻮـﻣﻮﻠﭘ ﮏـﯿﻧوﺮﮐ ﻪـﺑ نﺎـﯾﻼﺘﺒﻣ رد

سﻮﻠﯾژﺮﭙــﺳآ )

(CPA

دﻮﺑ ﺪــﻧ و ،ﺮــﺿﺎﺣ ﻪــﻌﻟﺎﻄﻣ فﻼــﺧ ﺮــﺑ

دﻮﺑ نﺎﻧز زا ﺶﯿﺑ نادﺮﻣ رد ﺖﻧﻮﻔﻋ )

29 .(

رد ﻪﻌﻟﺎﻄﻣ يا ﻪـﮐ

ﻂﺳﻮﺗ ﻪﺠﯾو ﺎﯾرﻮﺳ نارﺎﮑﻤﻫ و مﺎـﺠﻧا ﮏﯿﺘﺑﺎﯾد داﺮﻓا يور ﺮﺑ

،نارﺎـﻤﯿﺑ ﺐﻟﺎﻏ ﺖﯿﻌﻤﺟ ،ﺮﺿﺎﺣ ﻪﻌﻟﺎﻄﻣ ﺎﺑ ﻖﺑﺎﻄﺗ رد ،ﺪﺷ ﺎـﺑ

عﻮﯿﺷ 69

% ﺪﻧدﻮﺑ نﺎﻧز )

30 .(

ﻪﮐﺎﺠﻧآزا ﻪـﻧﻮﮔ يﺎـﻫ ﻒـﻠﺘﺨﻣ

لﺎﮕﻧﺎﻓ ﯽﺘﻧآ ﻞﺑﺎﻘﻣ رد سﻮﻠﯾژﺮﭙﺳآ ﺎﻫ

ﺖﻣوﺎﻘﻣ يﺎﻫ ﯽﺗوﺎـﻔﺘﻣ

نﺎﺸﻧ ﯽﻣ ﺪﻨﻫد

، ﻖﯿﻗد شور ﻪﺑ ﻪﻧﻮﮔ ﻦﯿﯿﻌﺗ ﯽﻣ

ﺪﻧاﻮﺗ ﮏﺷﺰﭘ

نﺎﻣرد ﺖﻬﺟ رد ار ﺪﻨﻤﻓﺪﻫ

ﺪـﻨﮐ ﺖﯾاﺪﻫ .

ﻪـﺘﻓﺎﯾ يﺎـﻫ ﻦـﯾا

ﻦﯿﻟﻮـﺑﻮﺗ ﺎﺘﺑ ﻪﯿﺣﺎﻧ ﯽﻟاﻮﺗ ﻦﯿﯿﻌﺗ شور ﻪﮐ داد نﺎﺸﻧ ﻪﻌﻟﺎﻄﻣ ﺎﺑ ﻪﺴﯾﺎﻘﻣ رد شور

يﺎﻫ ﻮﻣ يژﻮﻟﻮﻓر

، ﺮﺗﻻﺎـﺑ رﺎﯿـﺴﺑ ﺖﻗد زا و

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نﺎﻣز هﺎﺗﻮﮐ ﺮﺗ ﺰﯾﺎﻤﺗ ياﺮﺑ ﻪﻧﻮﮔ

يﺎﻫ رادرﻮـﺧﺮﺑ سﻮﻠﯾژﺮﭙـﺳآ

ﯽﻣ ﺪﺷﺎﺑ .

ﯽﻧادرﺪﻗ و ﺮﮑﺸﺗ

هرﺎﻤـﺷ ﻪـﺑ ﯽﺗﺎـﻘﯿﻘﺤﺗ حﺮـﻃ زا ﻞـﺻﺎﺣ ﺮﺿﺎﺣ ﻖﯿﻘﺤﺗ 394794 نﺎﻬﻔــﺻا ﯽﮑــﺷﺰﭘ مﻮــﻠﻋ هﺎﮕــﺸﻧاد رد بﻮــﺼﻣ

ﯽﻣ ﺪﺷﺎﺑ . ﯽـﺳﺎﻨﺷ چرﺎﻗ و ﻞﮕﻧا هوﺮﮔ مﺮﺘﺤﻣ ﻞﻨﺳﺮﭘ ﻪﯿﻠﮐ زا

ﺑ و ﻪﻧﺎﻤﯿﻤﺻ ﻪﮐ ﺮﺒﺻ ﺎ

ﻪﻠﺻﻮﺣ و ﺪﻧﺪﻧﺎﺳر يرﺎﯾ ار ﺎﻣ

، لﺎـﻤﮐ

ﻢﯾراد ار ﯽﻧادرﺪﻗ و ﺮﮑﺸﺗ .

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هرود ،ﻪﺘﻓﺎﯾ زﻮﻧ

د ،ﻢﻫ رﺎﻬﺑ 96 References

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د ،ﻢﻫ رﺎﻬﺑ 96

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Identification of morphological and molecular Aspergillus species isolated from patients based on beta-tubulin gene sequencing

Kheirkhah M¹, Chadeganipour M², Dehghan P³, Mohammadi R^*4

1. MSc of Medical Mycology, Department of Medical Parasitology and Mycology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

2. Professor, Department of Medical Parasitology and Mycology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

3. Assistant Professor, Department of Medical Parasitology and Mycology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

4. Assistant Professor, Department of Medical Parasitology and Mycology, Faculty of Medicine, Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran, [email protected]

Received:22 Jan 2017 Accepted:8 April 2017

Abstract

Background: Aspergillus species are opportunistic pathogens among immunocompromised patients. In terms of pathogenesis and mycotoxin production, they are in great value. The aim of the this study was to evaluate of beta-tubulin gene for identification of clinical Aspergillus species by PCR-sequencing method compared to morphological features of clinical isolates (such as conidial shape in direct microscopic examination, colony shape in culture, and physiological tests).

Materials and Methods: In this study, 465 patients referred to the Shefa laboratory of Isfahan were evaluated. Morphological and molecular identification of clinical samples were performed using culture on sabouraud agar, malt extract agar, czapekdox agar, direct microscopy, and PCR-sequencing of beta tubulin gene, respectively. Sequences were analyzed in comparison with gene bank data.

Results: Thirty nine out of 465 suspected cases (8.4%) had aspergillosis. The most prevalent species were Aspergillus flavus (56.4%), A. oryzae (20.5%), and A. fumigatus (10.2%), respectively. Fifty nine percent of patients were females and 49% were males.

Conclusion:In comparison with phenotypic tests, sequencing of beta-tubulin gene for identification of Aspergillus species is at great value. Replacement of molecular techniques with conventional tests is recommended for precise identification of microorganism for better management of infection.

Keywords:Aspergillus, Beta tubulin, Sequencing

*Citation: Kheirkhah M, Chadeganipour M, Dehghan P, Mohammadi R. Identification of morphological and molecular Aspergillus species isolated from patients based on beta-tubulin gene sequencing. Yafteh. 2017;19(1): 87-97.