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REFERENCES
1. Aebi, H. (1984). Catalase in vitro. Methods in Enzymology, 105, 121–126.
2. Asada, K. (1994). Production and action of active oxygen species in photosynthetic tissues. In: Foyer C.
& Mullineaux P.M. (eds), Causes of Photooxidative Stress and Amelioration of Defense Systems in Plants. CRC Press, Boca Raton, London, pp, 77–100.
3. Bailly C. (2004). Active oxygen species and antioxidants in seed biology. Seed Science Research, 14, 93–107.
4. Baum, M., Grando, S. & Ceccarelli, S.(2004). Localization of quantitative trait loci for d ryland characters in barley by linkage mapping. Challenges and Strategies for Dryland Agriculture, (vol. 32).
,( pp. 191–202). CSSA Special Publication.
5. Bor M., Özdemir F. & Türkan, I. (2003). The effect of salt stress on lipid peroxidation and antioxidants in leaves of sugar beet Beta vulgaris L. and wild beet Beta maritima L. Plant Science, 164, 77–84.
6. Bradford, M. M. (1976). Anual Biochemistry, 72, 248-254.
7. Chance, B. & Maehly, A. C. (1955). Assay of catalases and peroxidase. Methods Enzymol, 2, 764–775.
8. DadashiDooki A. and Mayer-posner F J, Askari H., Ziaee A. A., Salekdeh G H (2006) Proteomic response of rice young panicles to salinity. Proteomics, 6: 6498-6507.
9. Damerval, C., De vienne, D., Zivy, M. & Thiellement, H. (1986). Technical improvement in two- Dimentional electrophoresis increase the level of genetic variation detected in wheat-seedling proteins.
Electrophoresis, 7, 52-54.
10. Dat, J. F., Lopez–Delgado, H. Foyer, C. H. & Scott, I. M. (1998). Parallel changes in H2O2 and catalase during thermo tolerance induced by salicylic acid or heat acclimation in mustard seedlings. Plant Physiology, 116, 1351–1357.
11. Demiral, T., & Turkan, I. (2005). Comparative lipid peroxidation, antioxidant defense systems and proline content in roots of two rice cultivars differing in salt tolerance. Environmental and Experimental Botany, 53, 247–257.
12. Dionisio-Sese, M. L., & Tobita, S. (1998). Antioxidant responses of rice seedlings to salinity stress.
Plant Science (Limerick), 11135, 11–19.
13. Dietz , K.J., Jacob, S., Oelze, M. L., Laxa, M., Tognetti, V., Mariana, S., Miranda, N. D., Barier, M.
& Finkemeier, I. (2006). The function of peroxiredoxins in plant organelle redox metabolism. Journal of Experimental Botany, 57, 1697-1709.
14. Douce, R., Bourguignon, J., Neuburger, M. &Rebeille, F. (2001). The glycin decarboxylase system a fascinating complex. Trends Plant Science, 6, 167-176.
15. Fidalgo, F., Santos, A., Santos, I. & Salema, R. (2004). Effects of long–term salt stress on antioxidant defence systems, leaf water relations and chloroplast ultrastructure of potato plants. Annals of Applied Biology, 145(2), 185–192.
16. Hajheydari, M., Eivazi, A., Buchanan, B. B., Wong, J. H., Majidi, I. & Salekdeh, G. H. (2007).
Proteomics uncovers a role for redox in drought tolerance in wheat. Journal of Proteome Research, 6, 1451-1460.
17. Jiang, Y., & Huang, B. (2001). Drought and Heat Stress Injury to Two Cool-Season Turf grasses in Relation to Antioxidant Metabolism and Lipid Peroxidation. Crop Science, 41, 436–442.
18. Khanna-Chopra, R., & Selote, D. S. (2007). Acclimation to drought stress generates oxidative stress tolerance in drought-resistant than -susceptible wheat cultivar under field conditions. Environmental and Experimental Botany, 60, 276–283.
19. Kim, D. W.R., Agrawal, G. K., Jung, Y.H., Shibato, J., Jwa, N.S., Trahashi, Y., Iwahashi, H., Kim, D.H., Shim, I.S. & Vsui, K. (2005). Ahydrophobic rice seedling cukture model system for investigating proteome of salt stress in rice leaf. Electrophoresis, 6, 175-182.
20. Kitajima, S. (2008). Hydrogen Peroxide-mediated Inactivation of Two Chloroplastic Peroxidases, Ascorbate Peroxidase and 2-Cys Peroxiredoxin. Photochemistry and Photobiology, 84, 1404–1409.
21. Kubo, A., Aono, M., Nakajima, N., Saji, H., Tanaka, K. & Kondo, N. (1999). Differential responses in activity of antioxidant enzymes to different environmental stresses in Arabidopsis thaliana. Journal of Plant Research, 112, 279–290.
22. Lee, D. H., Kim, Y. S. & Lee, C. B. (2001). The inductive responses of the antioxidant enzymes by salt stress in the rice (Oryza sativaL.). Journal of Plant Physiology, 158, 737–745.
23. McDonald, M. B. (1999). Seed deterioration: physiology, repair, and assessment. Seed Science and Technology, 27(11), 177–237.
24. Mehlhorn, H., Lelandais, M., Korth, H. G. & Foyer, C. H. (1996). Ascorbate is the natural substrate for plant peroxidases. FEBS Letter, 378, 203–206.
25. Moradi ,F., & Abdelbaghi, M. I. (2007) . responses of photosynthesis, chlorophyll fluorescence and ROS-scavenging systems to salt stress during seedling and reproductive stages in rice. Annals of Botany, 99, 1161–1173
26. Moran, J. F., Becana, M. I., Iturbe-Ormaetxe, S., Frechilla, R., Klucas, V. & Aparicio-Tejo, P. (1994).
Drought induces oxidative stress in pea plants. Planta, 194, 346–352.
27. Naghib, A., Dong-Gi, L., Iftekhar, A., Piljoo, K., jeungjoo, L., Young-ock, A., Sang-soo, K., Ine-Jung, L., Jeong Dong, B., Kyu Young, K., Jenny, R., Setsuku, K. . & Byung-Hyun, L. (2008) . Comparative proteomic study of arsenic-induced differentially expressed proteins in rice roots reveals glutathione plays a central role during As stress. Proteomics, 8, 3561-3576.
28. Nakano, Y. & Asada, K. (1981). Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant and Cell Physiology, 22 (5), 867–880.
29. Noctor, G. & Foyer C. (1998). Ascorbate and glutathione: keeping active oxygen under control. Annual Reviews of Plant Physiology and Plant Molecular Biology, 49, 249–279.
30. Peskan-Bergh ◌fer, T., Neuwirth, J., Kusnetsov, V. & Oelmüller, R. (2005). ö Suppression of heterotrimeric G-protein â-subunit affects anther shape, pollen development and inflorescence architecture in tobacco. Planta , 220, 737–746.
31. Rajguru, S. N., Banks, S. W., Gossett, D. R., Lucas, M. C. & Millhollon, E. P. (1999) .Antioxidant response to salt stress during fiber development in cotton ovules. Journal of Cotton Science, 3, 11–18.
32. Salekdeh, G. H., Siopongco, J., Wade, L. J., Ghareyazie, B. & Bennett, J. ( 2002). Proteomic analysis of rice leaves during drought stress and recovery. Proteomics, 2, 1131–1145.
33. Schachtman, D.P. & Liu, W.H. (1999). Molecular pieces to the puzzle of interaction between potassium and sodium uptake in plants. Trends Plant Science, 4,281-287.
34. Shalata, A., Mittova, V., Volokita, M., Guy, M. & Tal, M. (2001). Response of the cultivated tomato and its wild salt-tolerant relative Lycopersicon pennellii to salt-dependent oxidative stress: The root antioxidative system. Physiologia Plantarum. 112, 487–494.
35. Shalata ,A., & Tal, M. (1998). The effect of salt stress on lipid peroxidation and antioxidants in the leaf of the cultivated tomato and its wild salt-tolerant relative Lycopersicon pennellii. Physiologia Plantarum, 104, 169–174.
36. Smith, I. K., Vierheller, T. V. & Thorne C. A. (1988). Assay of Glutathione reductase in crude tissue homogenates using 5,5'-Dithiobis (2-nitrobenzoic Acid). Analytical Biochemistry, 175, 408–413.
37. Streb, P. & Feierabend, J. (1996). Oxidative stress responses accompanying photoinactivation of catalase in NaCl–treated rye leaves. Botanica Acta, 109, 125–132.
38. Wang, J., Zhang, H. & Allen, R. D. (1999). Overexpression of an Arabidopsis peroxisomal ascorbate peroxidase gene in tobacco increases protection against oxidative stress. Plant Cell Physiology, 40, 725– 732.