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d D 1. Tripathi BK, Srivastava AK. Diabetes Mellitus:
Complications and Therapeutics. Med Sci Monit 2006;
12: RA130-47.
2. Biasibetti R, Tramontina AC, Costa AP, Dutra MF, Quincozes-Santos A, Nardin P, Bernardi CL, et al. Green tea (-) epigallocatechin-3-Gallate Reverses Oxidative Stress and Reduces Acetylcholinesterase Activity in a Streptozotocin-induced Model of Dementia. Behav Brain Res 2012; 236C:186-193. 3. Weaver C, Turner N, Hall J. Review of the Neuroanatomic Landscape Implicated in Glucose Sensing and Regulation of Nutrient Signaling:
Immunophenotypic Localization of Diabetes Gene Tcf7l2 in the Developing Murine Brain. J Chem Neuroanat 2012;45(1-2):1-17.
4. Parihar MS, Chaudhary M, Shetty R, Hemnani T.
Susceptibility of Hippocampus and Cerebral Cortex to Oxidative Damage in Streptozotocin Treated Mice:
prevention by Extracts of Withania Somnifera and Aloe vera. J Clin Neurosci 2004; 11:397-402.
5. Reagan LP, McEwen BS. Diabetes, but Not Stress, Reduces Neuronal Nitric Oxide Synthase Expression in Rat Hippocampus: Implications for Hippocampal Synaptic Plasticity. Neuroreport 2002; 13: 1801-1804.
6. Baydas G, Nedzvetskii VS, Nerush PA, Kirichenko SV, Yoldas T. Altered Expression of NCAM in Hippocampus and Cortex May Underlie Memory and Learning Deficits in Rats with Streptozotocin-induced Diabetes Mellitus. Life Sci 2003; 73:1907-1916.
7. Hung HY, Qian K, Morris-Natschke SL, Hsu CS, Lee KH. Recent Discovery of Plant-Derived Anti- diabetic Natural Products. Nat Prod Rep 2012;
29(5):580-606.
8. El-Tantawy WH, Hassanin LA. Hypoglycemic and Hypolipidemic Effects of Alcoholic Extract of Tribulus alatus in Streptozotocin-induced Diabetic Rats: a Comparative Study with T. Terrestris. Indian J Exp Biol 2007; 45: 785-90.
9. Guo Y, Shi DZ, Yin HJ, Chen KJ. Effects of Tribuli Saponins on Ventricular Remodeling after Myocardial Infarction in Hyperlipidemic Rats. Am J Chin Med 2007; 35: 309-16.
10. Amin A, Lotfy M, Shafiullah M, Adeghate E.The Protective Effect of Tribulus Tterrestris in Diabetes.
Ann N Y Acad Sci 2006; 1084: 391-401.
11. Liu XM, Huang QF, Zhang YL, Lou JL, Liu HS, Zheng H. Effects of Tribulus Terrestris L. Saponion on Apoptosis of Cortical Neurons Induced by Hypoxia- Reoxygenation in Rats. Zhong Xi Yi Jie He Xue Bao 2008;6(1):45-50.
12. Berkman Z, Tanriover G, Acar G, Sati L, Altug T, Demir R. Changes in the Brain Cortex of Rabbits on a Cholesterol-rich Diet Following Supplementation with a Herbal Extract of Tribulus Terrestris. Histol Histopathol 2009; 24(6):683-92.
13. Nasri S, Roghani M, Baluchnejadmojarad T, Balvardi M, Rabani T. Chronic Cyanidin-3-Glucoside Administration Improves Short-term Spatial Recognition Memory but Not Passive Avoidance Learning and Memory in Streptozotocin-diabetic Rats.
Phytother Res 2012; 26(8):1205-10.
14. Lupien SB, Bluhm EJ, Ishii DN. Systemic Insulin- like Growth Factor-I Administration Prevents Cognitive Impairment in Diabetic Rats, and Brain IGF Regulates Learning/Memory in Normal Adult Rats. J Neurosci Res 2003; 74:512-523.
15. Sima AA, Li ZG. The Effect of C-peptide on Cognitive Dysfunction and Hippocampal Apoptosis in Type 1 Diabetic Rats. Diabetes 2005; 54:1497-1505.
16. Biessels GJ, Ter Laak MP, Kamal A, Gispen WH. Effects of the Ca2+ Antagonist Nimodipine on Functional Deficits in the Peripheral and Central Nervous System of Streptozotocin-Diabetic Rats. Brain Res 2005; 1035:86-93.
17. Konar A, Shah N, Singh R, Saxena N, Kaul SC, Wadhwa R, Thakur MK. Protective Role of Ashwagandha Leaf Extract and Its Component Withanone on Scopolamine-Induced Changes in the Brain and Brain-derived Cells. PLoS One 2011;
6(11):e27265.
Effect of Tribulus Terrestris Oral Feeding on Learning and Memory in Streptozotocin-diabetic Rats: Investigating the Role of lipid Peroxidation
* Roghani M. (Ph.D)1- Omid Malayeri S. (Medical Stu.)2- Malayeri S.O. (Dental Stu.)3
*Corresponding Address: Neurophysiology Research Center, Shahed University, Tehran, IRAN Email: [email protected]
Received: 28/Dec/2011 Accepted: 29/Oct/2012
Abstract
Introduction: Diabetes in long term is associated with learning and memory deficits. Given the Anti-diabetic and antioxidant activities of Tribulus terrestris (TT).
Objective: to evaluate the effect of oral administration of Tribulus terrestris on learning and memory in diabetic rats.
Materials and Methods: Thirty two Wistar rats were divided into control, TT-treated control, diabetic, and TT- treated diabetic groups. To evaluate their learning and memory, initial (IL) and step-through latencies (STL) were determined at the end of study using passive avoidance test and alternation behavior percentage was obtained using Y maze Level of malondialdehyde in hippocampal homogenate was also measured at the end of the study.
Results: STL significantly decreased in diabetic group (p<0.01) and TT-treated diabetic group showed a significantly higher STL (p<0.01), compared to the diabetic counterparts. Alternation percentage was significantly lower in the diabetic group relative to the controls (p<0.01) and treated diabetic group did show a significant difference in comparison with the diabetic group (p<0.05). Diabetic rats also exhibited a significant increase in tissue level of malondialdehyde (p<0.01) and TT treatment significantly reduced MDA level (p<0.01) in the diabetic group.
Conclusion: TT treatment could enhance the capability of consolidation and recall in diabetic animals and could also improve spatial memory in them, partly via attenuation of lipid peroxidation in the hippocampus.
Key words: Diabetes Mellitus/ Learning/ lipid peroxidation /Memory Tribulus
______________________________________Journal of Guilan University of Medical Sciences, No: 85, Pages: 88-95
1. Neurophysiology Research Center, Shahed University, Tehran, IRAN