482.
3) Hsu, H. J., Liang, M. R., Chen, C. T., and Chung, B. C.
(2006) Pregnenolone stabilizes microtubules and pro- motes zebrafish embryonic cell movement. Nature439.
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Fig. 1. Sulfation of nitrotyrosine catalyzed by the human SULT1A3 with PAP[35S] as the sulfate donor. The figure shows the autoradiograph taken from the TLC plate used for the two- dimensional thin-layer analysis of the reaction mixture. The dashed line circle indicates the position of enzymatically synthesized nitrotyro- sineO-sulfate revealed by ninhydrin staining.
Table 1. Nitrotyrosine or dopamine-sulfating activities of the crude cell lysate in HepG2 human hepatoma cells transfected with SULT1A3- specific siRNAs.
Specific Activity (pmol/min/mg cellular protein) Nitrotyrosine Dopamine Control 2.02¼0.35 (100½) 26.7¼1.0 (100½) 0 nM siRNA 2.15¼0.32 (106.4) 28.0¼1.5 (104.5)
0.5 nM 1.96¼0.24 (96.8) 23.6¼0.2 (88.3) 5 nM 1.39¼0.14 (68.8) 22.3¼1.0 (83.2) 50 nM 1.44¼0.36 (71.2) 20.9¼1.1 (78.2) 100 nM 1.27¼0.05 (62.6) 20.6¼2.0 (76.9) Results represent means¼SD derived from three experiments. Data in the parentheses indicate the ratio of the activity of the crude cell lysates in HepG2 cells transfected with SULT1A3-specific siRNA versus control.
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