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Summary

By the use of light microscopy it has been revealed, that intensively stained degradated cells of embryogenic calli have characteristic features of cells with signs of programmed cell death:

destruction of a nuclear material and output in cytoplasm, shrinkage of cytoplasm, presence of periplasmatic space between the cytopasm and the cell wall, thickened cell wall, increase of cells sizes, high activity of oxidative-reductative processes and decrease of cytoplasm . Additionally, destruction of nuclear material and internucleosomal fragmentation of DNA molecules, which are appeared to be a main attributes of apoptosis, has been revealed by the use of fluorescent microscopy and staining by acridine orange and according the TUNEL method.

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