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Institute of Systems Biology (INBIOSIS), UKM

WORKSHOP SUMMARY

Many Bioscience students and professionals find the concepts of enzymology difficult to understand.

The aim of this workshop therefore is to provide an in-depth revision of the basic concepts of enzymology; and to expand these concepts to the actions of enzymes within metabolic pathways. In this workshop we will discuss the implications of the irreversible and reversible Michaelis Menten equation and its implications on enzymatic rates within linear biochemical pathways. We will look at how typical enzyme parameters can be derived from experimental data. Furthermore, we will investigate different reversible inhibitor types and how they affect metabolic pathways. The general concepts of enzymology will be further expanded to 2-substrate reactions and how they can be analysed. We will discuss the function and behaviour of allosteric enzymes and investigate their regulations within a metabolic context. With a thorough understanding of these concepts we will then explore, how enzymes can perform complex functions within a systems biology context. For this we will look at selected systems containing feedback and feed forward loops and analyse the concept of zero- order ultra-sensitivity, resulting in ‘futile cycles’. Finally, we will discuss the allosteric regulation of complex pathways, which lead to emergent behaviour of metabolic pathways. Examples of pathways will be illustrated through systems biology modelling with JDesigner.

Attendance confirmation should be made no later than 28 June 2012 and sent to:

Institute of Systems Biology (INBIOSIS) Universiti Kebangsaan Malaysia

43600 UKM Bangi Selangor Darul Ehsan or faxed to 03-8921 3398 Enquiries:

Nur Hasrina Mohar /General Tel: 03-8921 4558 / 4549

Email: [email protected]/ [email protected] DATE

2-3 July 2012

WORKSHOP

ENZYMES AND THEIR ACTIONS WITHIN THE METABOLIC CONTEXT

BY

Dr. Peter Klappa

University of Kent, UK

VENUE

Institute of Systems Biology, Universiti Kebangsaan Malaysia Bangi, Selangor

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Institute of Systems Biology (INBIOSIS), UKM

REGISTRATION FORM Workshop

ENZYMES AND THEIR ACTIONS WITHIN THE METABOLIC CONTEXT By

Dr. Peter Klappa (University of Kent, United Kingdom)

Name

(Prof./Assoc. Prof./Dr./Mr./Mrs./Ms.)

: Organization:

Address:

E-mail: Tel:

Workshop Fees: RM200

(Registration fee covers workshop materials, lunch, coffee/tea break and certificate must be paid in full on the day of registration)

Method of Payment:

Cash Cheque,

payable to:

Bendahari Universiti Kebangsaan Malaysia

*Note: Participants are advised to provide your own laptop and please download and install a freeware JDesigner programme from:

Date: Signature:

*As places are limited, early registration is recommended

Institute of Systems Biology (INBIOSIS)

Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia Tel: +603 8921 4558/4549 │ Fax: +603 8921 3398

[email protected] http://www.inbiosis.ukm.my

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