FUNDAMENTAL OF MASS SPECTROMETRY
& THE APPLICATION OF MASS SPECTROMETRY IN PLANT ANALYSIS ASSOC. PROF. DR. POLKIT SANGVANICH By
Chulalongkorn University, Thailand
INBIOSIS
SEMINAR SERIES 21
TENTATIVE SCHEDULE 12/09/2012 8:30 am – 9:00 am
Registration 9:00 am – 10:30 am
Welcome Speech
Seminar 1: Fundamental of Mass Spectrometry 10:30 am – 11:00 am
Coffee Break 11:00 am – 12.30 noon
Seminar 2: The Application of Mass Spectrometry in Plant Analysis
Conclusion, Q&A and Wrap-up Lunch
12 SEPTEMBER 2012 (Wednesday) 8.30 am ~ 12.30 noon
INBIOSIS Seminar Hall
UKM, Bangi
FUNDAMENTAL OF MASS SPECTROMETRY
& THE APPLICATION OF MASS
SPECTROMETRY IN PLANT ANALYSIS
ABS T RA CT
This presentation is based on an overview of the energetically developing field of mass spectrometry-based of proteomics and metabolomics. Proteomics is the high-throughput analysis of complexes mixtures of proteins. The aim of the proteomic is to explain the biological systems of each organism which is very useful for plant biology. While nowadays, there is new technology that is successor of proteomics call metabolomics.
The Metabolomics aims at the comprehensive and quantitative analysis of wide arrays of metabolites in biological samples. These various analytes have very diverse chemical properties and occur at different abundance levels.
Consequently, comprehensive metabolomics investigations are primarily a challenge for analytical chemistry and specifically mass spectrometry has vast potential as a tool for this type of investigation. Metabolomics require special approaches for sample preparation, separation, and mass spectrometric analysis. Current examples of those approaches are described in this review. It principally focuses on fingerprinting; a technique that analyzes all detectable analytes in a given sample with subsequent classification of samples and identification of differentially expressed metabolites, which define the sample classes. To perform this complex task, data analysis tools, metabolite libraries, and databases are required. For example, the describe a detailed protocol for large-scale metabolomics of plant tissues, based on reversed phase liquid chromatography coupled to high-resolution mass spectrometry (LC-QTOF MS) of aqueous methanol extracts.
Subsequent statistics and bioinformatics tools can be used to provide a detailed view on the differences and similarities between (groups of) samples or to link metabolomics data to other systems biology information.
Research Centre of Bioorganic Chemistry (RCBC), Department of Chemistry, Faculty of Science, Chulalongkorn University, Pathumwan, Bangkok, 10330, THAILAND
Tel: +662-2187637 Fax: +662-2187598 Academic Position
Associate Professor of Chemistry Executive Position
- Head of Institutional Biosafety Committee (IBC), Chulalongkorn University
- Director Institute of Biotechnology and Genetic Engineering, Chulalongkorn University (2010-Present)
-Deputy Dean of Planning and Development, Faculty of Science, Chulalongkorn University (2009-2010)
Publications
1. Yodjun, M., Karnchanatat, A. & Sangvanich, P. (2012) Angiotensin I- Converting Enzyme Inhibitory Proteins and Peptides from the Rhizomes of Zingiberaceae Plants, Appl Biochem Biotech.
2. Panuthai, T., Sihanonth, P., Piapukiew, J., Sooksai, S., Sangvanich, P. & Karnchanatat, A. (2012) An extracellular lipase from the endophytic fungi Fusarium oxysporum isolated from the Thai medicinal plant, Croton oblongifolius Roxb., Afr J Microbiol Res. 6, 2622-2638.
3. Moon-Ai, W., Niyomploy, P., Boonsombat, R., Sangvanich, P. &
Karnchanatat, A. (2012) A Superoxide Dismutase Purified from the Rhizome of Curcuma aeruginosa Roxb. as Inhibitor of Nitric Oxide Production in the Macrophage-like RAW 264.7 Cell Line, Appl Biochem Biotech.
ASSOC. PROF. DR.
POLKIT SANGVANICH E-mail:
[email protected], [email protected]
SPEAKER
Attendance confirmation should be made no later than 5 September 2012 (Wed) and sent to:
Institute of Systems Biology Universiti Kebangsaan Malaysia 43600 UKM Bangi or
faxed to 03 8921 3398
Registration Fee: RM 50 (Method of Payment: Cash )
