Co-organized by
Pusat Analysis Teknologi Gen, INSTITUT BIOLOGI SISTEM
Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor
Speaker’s Profile
Name : Dr Caine Leong Tuck Choy
Dr Caine Leong works as Product Manager for Gene Expression Division at Bio-Rad Laboratories, supporting the assay design and optimization efforts of scientists working with RNAi, PCR and Quantitative PCR (Real-Time PCR) throughout Southeast Asia and Taiwan. Guest lecturer at Ngee Ann Polytechnic Singapore. Real-Time PCR Trainer for World Health Organization (WHO) Avian Influenza laboratories in Indonesia, Vietnam and Thailand.
Dr Caine Leong specialized in Gene Silencing, Gene Cloning, Gene Expression and Real-Time PCR applications. Research expertise include molecular cloning, DNA sequencing, DNA Microarray (Affymetrix), Real-time PCR, gene silencing using RNAi, expression of recombinant proteins, tissue culture, flow cytometry for apoptosis and cell cycle analysis, cytotoxicity assays, ELISA assays, etc.
Dr Caine Leong was been awarded 1st prize for oral presentation at the 5th Combined Annual Scientific Meeting 2004 (“Life Sciences in Singapore.”), National University of Singapore.
In year 2003, he was been awarded AVON international scholar-in-training award at the 94th American Association for Cancer Research (AACR), Washington D. C. and awarded 2nd prize for oral presentation at the 3rd Annual Graduate Student Society-Faculty of Medicine (GSS-FOM) Meeting 2003, National University of Singapore.
Location Map:
Location:
Pusat Analysis Teknologi Gen
INSTITUT BIOLOGI SISTEM (INBIOSIS) Universiti Kebangsaan Malaysia
43600, Bangi, Selangor.
For more information, please contact:
1. Cik Faridda Hanim/ Pn. Rafidah Ahmad Phone: 03-89214561/4558/4562
Email: [email protected]/[email protected] Fax : 03-89213398
2. Ms Sylvia Chang Phone: 016-3928337
Email: [email protected]
Real-time PCR Workshop
Venue: Pusat Analysis Teknologi Gen INSTITUT BIOLOGI SISTEM
Universiti Kebangsaan Malaysia 43600, Bangi, Selagor.
Date: 12 & 13 August 2009
Time: Day 1: 9:15 am - 5:15 pm
Day 2: 9:30 am – 5:15 pm
Co-organized by
Pusat Analysis Teknologi Gen, INSTITUT BIOLOGI SISTEM
Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor
Synopsis
Real-time polymerase chain reaction, also called as quantitative real time polymerase chain reaction (qPCR). It is a laboratory technique based on the polymerase chain reaction (PCR), which is used to amplify and simultaneously quantify a targeted DNA molecule. It enables both detection and quantification of a specific sequence in a DNA sample.
There are two common methods of quantification which included the use of fluorescent dyes that intercalate with double-stranded DNA, and modified DNA oligonucleotide probes that fluoresce when hybridized with a complementary DNA.
Frequently, real-time polymerase chain reaction is combined with reverse transcription to quantify messenger RNA (mRNA) in cells or tissues.
In molecular biology High Resolution Melt (HRM) analysis as it will be referred to herein is a hugely powerful technique for the detection of mutations, polymorphisms and epigenetic differences in double stranded DNA samples. It has advantages over other genotyping technologies.
Objective
This workshop aims to provide basic concepts of the gene expression workflow by using the real-time PCR as well as further understanding on the technology of High Resolution Melt (HRM).
Registration Fee
Each Participant RM 200 cash on the registration.
Workshop Agenda
DAY 1: 12 Aug 2009 (Wednesday)
8.30 - 9.00 am Registration
9.15 - 10.30 am Introduction to Real-Time Polymerase Chain Reaction
10.30 - 10.45 am Tea break
10.45 - 12.15 am High Resolution Melt (HRM): Post- PCR Melt Study
(By Dr Caine Leong, Ph.D.) 12.15 - 12.45 pm Q & A Session
12. 45 - 1.45 pm Lunch
1.45 - 2.15 pm Briefing of the Experiment
2.15 - 3.30 pm Setting Up of Experiment and Running of Assay
3.30 - 4.00 pm Real-Time PCR Software Walkthrough I (CFX Manager) 4.00 - 4.15 pm Tea break
4.15 - 5.15 pm Life Science Technology Platform Part I (Real-Time PCR Instruments and Related Chemistries)
DAY 2: 13 Aug 2009 (Thursday)
9.30 - 10.30 am Real-Time PCR Software Walkthrough II (iQ5 Software) 10.30 - 10.45 am Tea break
10.45 - 11.45 am Life Science Technology Platform Part II (Pre-PCR Sample Preparation:
Macherey-Nagel Extration Kit and OMNI Homogenizer)
11.45 – 12.45 pm Primer and Probe Design:Guideline qPCR Pitfalls: Learn and avoid 12.45 - 1.45 pm Lunch
1.45 - 3.00 pm Software for Primer and Probe Design
3.00 - 4.00 pm Life Science Technology Platform Part III (The Automated Partner of Real-Time PCR: Experion) 4.00 - 4.15 pm Tea
4.15 - 5.15 pm Assay Results and Discussion
END OF WORKSHOP
