Apart from the long-term storage and preservation of germplasm for future generations, utilizing the vast collection of germplasm has always been a challenge for any gene bank. Collecting and preserving this rice germplasm will facilitate the protection of genetic wealth, thereby preserving the rich diversity of the Philippine rice germplasm. With the advent of the Plant Variety Protection Act, access, exchange and sharing of the benefits of the conserved rice germplasm will require legal instruments such as the Standard Material Transfer Agreement (SMTA) that will be established for the protection of genetic resources of Philippine rice.
The wider range and diversity of materials can be used for variety improvement if more rice germplasm accessions and information are available. A total of 707 germplasms were received for safety duplication of rice germplasm from PhiliRice Batac (27) and PhilRice Midsayap (685). The study aims to conserve rice germplasm resource for medium and long term storage and rejuvenate low stocks and low viability rice germplasm for conservation and distribution.
The data obtained can serve as a basis for planning the regeneration of rice germplasm to obtain sufficient seed stocks. GEDS maintains accurate, reliable and up-to-date rice germplasm information, facilitating data search and retrieval for better germplasm access and use. GEDS containing important rice germplasm data was updated: passport data (960 collections), characterization data (172 entries for 2014DS and 547 entries for 2014WS), grain quality data (885 collections/accessions), rice tungro field evaluation data (967 collections/accessions) and storage data ( 4,074 accessions).
Northeast Luzon (NE) has an extensive collection of rice germplasm that contains a diverse source of important and desirable traits.
Evaluation of PhilRice Germplasm Collection for Biotic and Abiotic Stresses in Irrigated Lowland and Grain Quality
Evaluation of the PhilRice germplasm collection for biotic and abiotic stresses in irrigated lowlands and grain quality. PRRI003166) and Ortoc (PRRI002867) genetic material showed higher shoot biomass and yield under different gradients and intensities of water deficit stress. Five rice germplasm accessions identified with tolerance to soil zinc deficiency with good to excellent phenotypic performance, these are: Minaangan (Coll. Evaluation of PhilRice germplasm collection for biotic stress JP Rillon, GDC Santiago and MSV Duca.
Rice germplasm has useful genes for key traits such as resistance to insect pests and diseases. This rice germplasm should be continuously screened for resistance to blast, bacterial leaf blight (BLB), sheath blight (ShB), rice tungro virus (RTV), green leafhopper (GLH), brown leafhopper (BPH) and columnar borer. Summary table of reactions of PhilRice germplasm accessions to major diseases and insect pests 2015 DS.
Evaluation of PhilRice germplasm collection for grain quality RC de Leon, APP Tuaño, MC Ferrer, MIC Calayugan and LM Perez. This ongoing study aims to generate grain quality data for the PhilRice germplasm collection and efficiently provide grain quality data through a computerized database system managed by the PhilRice Genetic Resources Division (GRD). Evaluation of PhilRice germplasm collections for abiotic stress JM Niones, MCN Julaton and RR Suralta.
This study aimed to evaluate and screen PhilRice germplasm collections under different intensities of drought stress using line source spray system. Over one hundred and two germplasm accessions were transplanted and performed in the waterproof experimental bed with line source sprinkler system under a rain shelter. Soil moisture sensors were placed on both sides of the seedbed at different distances from the line sprayer (10cm, 40cm, 80cm, 120cm, 160cm), respectively.
List of germplasm evaluated under different intensities of drought stress using the Line Source Sprinkler (LSS) system in 2015. Five rice germplasm accessions identified with tolerance to soil zinc deficiency with good to excellent phenotypic performance , these are: Minaangan (Coll. 10 germplasm orice stocks identified with moderate resistance to zinc deficiency comparable to IR10A183 (a tolerant genotype).
Currently, cultivars ARC 11554, Utri merah, Utri Rajapan, Habiganj DW8 and some wild rice relatives are used as resistant donors for RTV breeding programs at PhilRice, but little attention has been paid to searching for new sources of resistance from our PhilRice germplasm. Moreover, characterizing and identifying our own materials for potential source of disease resistance genes will mean protecting these heirloom plant materials from inappropriate, misuse and unauthorized exploitation, with the growing attention given to intellectual property rights issues, especially regarding ownership of rice germplasm under development. The number of plant accessions to be subjected to phenotypic and molecular characterization of host resistance will largely depend on the field evaluation results of the PhilRice germplasm collection for biotic stresses.
Genetic Resources Research
The focus of the study is to determine potential founder lines in the rice germplasms preserved at PhilRice genebank. Selection of founder lines to be included in the MAGIC population will be done with the breeders and considering unique agronomic traits suitable for the needs of rice farmers in the Philippines. Philippine traditional rice varieties currently preserved in PhilRice Genebank have enormous genetic diversity and potential new genes for rice genetic improvement.
With the advent of intellectual property rights and ownership of rice, especially developing germplasm, there is a need to discover local sources of genes or traits for breeding and genetic improvement of rice varieties for pest and disease resistance, abiotic stress resistance and good grains. quality. The discovery of genes and the potential source of germplasm in traditional local and indigenous rice varieties will mean opportunities for the commercialization of rice science progress in the Philippines. In the 2015 dry season (DS), four traditional Philippine rice varieties were selected based on morpho-agronomic traits and evaluation for resistance or tolerance to biotic and abiotic stresses.
FastQC (version 0.11.3) results of three Philippine traditional rice varieties were obtained from PGC-Core Bioinformatics Facility in UP-Diliman, Quezon City. The green color on the y-axis represents very good quality of calls, the orange color is fair. quality calls and red as poor in quality calls. Investigation of the bound form of 2-acetyl-1-pyrroline (2-AP) in Philippine aromatic rice in the PhilRice Gene Bank.
Aromatic rice with superior eating quality commands higher price in the market and has been increasingly demanded by niche markets abroad. The popular Thai jasmine rice "Khao Dawk Mali 105" and Pakistani "Basmati" rice have both been recognized worldwide for their distinctive aroma, appearance and eating quality and continue to be highly valued in the international market. Molecular analysis of the DA export rice (2015DS crop) using the Bradbury marker for the scent gene (fgr) has been completed.
Surprisingly, waxy rice varieties Malagkit Sungsong, Improved Malagkit Sungsong 2 and UPLRi-1 gave non-fgr results. In the Philippines, rice has always been a strong focal point in Filipino culture and traditions and agriculture-based research. This study aims to collect and preserve traditional rice varieties and gather information from local communities about their use of rice in folk medicine.
Optimization of Germplasm Conservation Procedures
Among the physiological and morphological characteristics, grain shape and grain phenolic reaction have been widely used as conventional tools to classify rice cultivars into Japonica and Indica species.
Conservation of Genetic Resources in the Rice Environment
The main purpose of establishing the azole germplasm collections at PhilRice is to conserve Philippine indigenous azole species and stocks widely adapted to Philippine conditions for use. Azolla accession list available in the PhilRice Azolla Germplasm Collection as of December 31, 2015 for distribution. Ten isolates of Beauveria bassiana (Table 19), 10 isolates of Metarhizium anisopliae (Table 20) and one isolate of Paecilomyces sp.
After culture recovery, the mycelia of the fungal isolates grew well in culture medium and thus remained viable (Table 21). With increasing awareness of the undesirable human and environmental impacts of the use of inorganic fertilizers, herbicides and pesticides, PhilRice through its R&D programs had long recognized that beneficial microbes provide an alternative strategy to combat the limiting soil nutrients and destructive effects of weeds and pests to combat. on crops. Preservation methods of potentially important isolates for agrobiological applications should be optimized early in the product development process to avoid potential economic and scientific losses in the event of decline of a production strain.
Dual culture analysis between the sheath blight pathogen Rhizoctonia solani (right side of plate) and Trichoderma sp. left side of the plate) that has been subjected to different storage conditions. Effect of different storage conditions on indole-3-acetic acid (IAA) production activity of Streptomyces mutabilis. Effect of different storage conditions on 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity of Streptomyces mutabilis.
Growth and sporulation of isolates are indicative of ACC utilization and ACC deaminase production. Effect of different storage conditions on indole-3-acetic acid (IAA) production activity of Streptomyces mutabilis. Growth and sporulation of isolates are indicative of ACC utilization and ACC deaminase production.
