Bioassay Assessment, Fractionation, and High Performance Liquid Chromatography (LC/MS) Analysis of Macro-Sorb Foliar.
(4181)
Authors:
G.L. Kauffman III* - The Pennsylvania State University D.A. Jones - The Pennsylvania State University
T.L. Watschke - The Pennsylvania State University
Abstract:
Growth responses elicited by one biostimulant, Macro-Sorb Foliar (Foliar), alone or in separate fractions based on solubility were investigated using three in vitro bioassays specific and sensitive to auxins, cytokinins, and brassinosteroids. Auxin-like responses were tested using the Avena (oat) first internode straight growth bioassay. Cytokinin-like responses were tested using the Raphanus (radish) cotyledon expansion bioassay, and brassinosteriod-like responses were tested using the rice lamina inclination bioassay. Nitrogen controls using concentrations equivalent to that contained within Foliar were utilized in all bioassay trials. Foliar elicited plant responses similar to auxin in all trials which were significantly different from the nitrogen controls. Moreover, the lipophilic fraction elicited a significantly greater response that both the water-soluble fraction and nitrogen control. Foliar did not elicit a plant response similar to cytokinins. The lipid soluble fraction of Foliar elicited plant responses similar to brassinolide using the rice lamina inclination bioassay. Liquid chromatography (LC/MS) analysis revealed that compounds with a similar molecular weight (~ 450 m/z) and number of carbons (26) to sterol molecules such as brassinolide were present in each of the active fractions. These results suggest that Foliar might contain a biologically active compound which is sterol (hydrophobic) in nature.
Speaker Information: Gordon Kauffman III, The Pennsylvania State University, 116 ASI Building, University park, PA 16803; Phone: 814 880 8873; E-mail: [email protected]
Session Information: Thursday, November 4, 2004, 7:55 AM-10:35 AM Presentation Start: 8:15 AM
Keywords: Macro-Sorb Foliar; rice lamina inclination bioassay; high perfomance liquid chromatography;
brassinosteroids