Characterization of soil microbial biodiversity under alternative land management programs for tomato production. (4504)
Authors:
T. Wu* - USHRL-ARS-USDA and CREC,University of Florida D.O. Chellemi - USHRL-ARS-USDA, Fort Pierce, FL
K.J. Martin - The University of West Florida, Pensacola, FL J.H. Graham - CREC, University of Florida, Lake Alfred, FL E.N. Rosskopf - USHRL-ARS-USDA, Fort Pierce, FL
G.T. Church - USHRL-ARS-USDA, Fort Pierce, FL
Abstract:
Length Heterogeneity-Polymerase Chain Reaction (LH-PCR) and Terminal Restriction Fragment Length Polymorphisms (T-RFLP) of fungal and bacterial rDNA were employed to study soil microbial biodiversity in fields managed for three years under the following land management programs: conventional tomato production using soil fumigation with 1,3-dichloropropene plus chloropicrin, preparation for organic production utilizing cover crops and organic amendments, weed fallow, disk fallow, and establishment of bahiagrass pasture. Univariate analysis of community structure indicated a significantly higher diversity of ITS-1 region fungal rDNA in soils following three years of weed fallow or bahiagrass pasture. Multivariate analysis indicated the composition of ITS-1 region fungal rDNA was unique for each land management program except conventional tomato production and disk fallow, which had a similar composition of rDNA obtained from the ITS-1 region. Univariate analysis indicated the diversity of bacterial rDNA in soils after three years of bahiagrass pasture was significantly lower than all other land management programs except weed fallow. Multivariate analysis indicated a high degree of similarity in the composition of bacterial rDNA between bahiagrass pasture and weed fallow.
Speaker Information: Tiehang Wu, USHRL-ARS-USDA and CREC,University of Florida, 2001 South Rock Road, Fort Pierce, FL 34945; Phone: 772-462-5849; E-mail:
Session Information: Tuesday, November 2, 2004, 8:00 AM-10:00 AM Presentation Start: 8:00 AM (Poster Board Number: 2707)
Keywords: Fungal communities; Bacterial communities; LH-PCR; T-RFLP