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Key words:.RSDNONQNRHR2@MCAK@RSDC@MC@BHCDSBGDCSHS@MHTLRTQE@BD.RSDNAK@RSHBCHEEDQDMSH@SHNM
*Graduate Student, Department of Oral & Maxillofacial Surgery, Faculty of Dentistry, Prince of Songkla University, Hat Yai, Songkhla, Thailand
**Associate Professor, Department of Oral & Maxillofacial Surgery, Faculty of Dentistry ,Prince of Songkla University, Hat Yai, Songkhla, Thailand
***Associate Professor, Department of Orthopaedic Surgery & Physical Medicine, School of Medicine, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, Thailand
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/QDLIHS QONQML@DJKNMF
SWU Dent J. Vol.7 Suppl 2014
3GDBTQQDMSRSTCX@HLDCSNHMUDRSHF@SD DEEDBSRNE2+ SHS@MHTLRTQE@BDRNMFQNVSG@MC NRSDNFDMHBCHEEDQDMSH@SHNMNEG!,2"RHM$#BDKK BTKSTQD
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Figure 1. Demonstrating morphologies of hBMSCs (A) at primary, (B) 3rd passage, and scanning electron microscope images of (C) smooth and (D) sandblasted and acid-etched
(SLA) titanium surfaces. Insets represent (C) smooth and (D) SLA titanium disks.
HM $#FQNVSG LDCHTL ENQ GQ ESDQ SG@S BTKSTQDLDCHTLV@RBG@MFDCSNQDFTK@Q%!2@MC
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(MUDRSHF@SHNMR
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@MC ENQL@KCDGXCD CDGXCQ@SDC HM DSG@MNK RDQHDRNE@HQCQHDCFNKCROTSSDQBN@SDC
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Cell viability assay:"DKKUH@AHKHSXV@R LD@RTQDCSNQDOQDRDMSBDKKFQNVSG.MC@X BDKKRVDQDHMBTA@SDCHM"DKK3HSDQ® PTDNTR .MD2NKTSHNM"DKK/QNKHEDQ@SHNM RR@X/QNLDF@
42 HM OGDMNK QDC EQDD BTKSTQD LDCHTL VHSG
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SWU Dent J. Vol.7 Suppl 2014
RR@X*HS!HN1@C42 ENKKNVHMFL@MTE@BSTQDQÄR HMRSQTBSHNM"DKKKXR@SDV@RHMBTA@SDCVHSG O@Q@MHSQNOGDMNKOGNROG@SDR2HFL@XHDKCHMF@
XDKKNVRTARSQ@SDNEOMHSQNOGDMNK"NKNQHMSDMRHSX V@R QD@C @S ML @ARNQA@MBD HM CTOKHB@SD ,TKSHRJ@M&.:<
For calcium content assay:"DKKODKKDSR EQNLBDKKKXR@SDRVDQDCDLHMDQ@KHYDCNUDQMHFGSHM ,'"+@S13SGDMKDUDKRNEB@KBHTLBNMSDMSR VDQDLD@RTQDCTRHMF"@KBHTL"NKNQHLDSQHB RR@X JHS !HNUHRHNM 42 ENKKNVHMF @ L@MTE@BSTQDQÄR HMRSQTBSHNM"NMBDMSQ@SHNMRNEOMHSQNOGDMNK@MC B@KBHTLBNMSDMSRVDQDDWSQ@ONK@SDCVHSGRS@MC@QC BTQUDR NE RDQH@K CHKTSHNMR NE OMHSQNOGDMNK @MC B@KBHTLRS@MC@QCQDRODBSHUDKXSGDMMNQL@KHYDC AXSGD@LNTMSNEOQNSDHMBNMSDMSRNESGDR@LD R@LOKDR @MC QDONQSDC @R M, OMHSQNOGDMNK LF OQNSDHM @MC MF B@KBHTLLF OQNSDHM M ,D@M±2#:<
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@R @OOQNOQH@SD .SGDQVHRD SGD *QTRJ@K6@KKHR
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@MCDRSQNFDMCDOQHUDCBNMCHSHNM
Scanning electron microscope images CDLNMRSQ@SDCCHEEDQDMSBDKKRG@ODRNMCHEEDQDMS SHS@MHTLRTQE@BDR@MC@KNVDQBDKKCDMRHSXHM$#
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BDKKRRSQDSBGDCBDKKANCX@BQNRRQNTFGRTQE@BDR AX DWSDMCHMF RL@KK BXSNOK@RLHB OQNBDRRDR SN
@SS@BG NM ONQNTR RTQE@BD BQD@SHMF HMSDQBDKKTK@Q MDSVNQJ%HF"#+NVDQBDKKCDMRHSXNM RLNNSG@MC2+ RTQE@BDRHM$#.2%HF!#
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Figure 3. Demonstrating cell growth derived from cell viability assay of hBMSCson day 21, on cell culture plate (Pl) and smooth (SM) and sandblasted and acid-etched (SLA) titanium
surfaces in conventional (FBS) and estrogen-deprived (ED) growth media. Symbols + represents significantly lower than cell culture plate, *, significantly lower than FBS-OS on the
same cell culture substrates, and † significantly lower than other surfaces in ED-OS and FBS-OS media, at p<0.05 (n=4, Mean±SD).
Figure 2. Scanning electron microscope images of hBMSCs on smooth and SLA titanium surfaces on day 7 (A&B) on smooth and (C&D) sandblasted and acid-etched (SLA) titanium
surfaces, (A&C) in conventional (FBS-OS) and (B&D) estrogen-deprived osteogenic media (ED-OS). Inset images on C and D exhibit cell attachment on the SLA titanium surface.
SWU Dent J. Vol.7 Suppl 2014 KJ@KHMDOGNROG@S@RD +/@BSHUHSX@MC
B@KBHTLBNMSDMSKDUDKRNEG!,2"RNM@KKRTQE@BDR HM$#.2VDQDRHFMHEHB@MSKXKNVDQSG@M%!2.2 LDCH@O%HF ! KJ@KHMDOGNROG@S@RD
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%HF!
Figure 4. Demonstrating osteogenic differentiation markers of hBMSCs, (A) alkaline phosphatase activity and (B) calcium contents on cell culture plate (Pl) and smooth (SM) and
sandblasted and acid-etched (SLA) titanium surfaces in conventional (FBS-OS) and estrogen-deprived osteogenic media (ED-OS) on day 21. Symbols *represents significantly
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SWU Dent J. Vol.7 Suppl 2014
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