A Greenhouse Method for Screening Soybean Genotypes for Tolerance to Soil Chloride. (C01-shannon101577-poster)
Authors:
J.G. Shannon* - Univ. of Missouri D.J. Dunn - Univ. of Missouri D.D. Widick - Arkansas State Univ.
J.M. Dunn - Arkansas State Univ.
Abstract:
Salt toxicity is a factor that limits world soybean production. Damage in the US is often a result of irrigation with water high in salt content. Soybean genotypes differ in ability of roots to exclude chloride ions. Cultivars that are chloride excluders are considered salt tolerant and exclude chloride from roots.
Cultivars that are chloride includers are salt intolerant and accumulate chloride ions that in excess cause plant injury. A hydroponic system of growing plants and adding salt to the solution at V5 growth stage is a common method of screening genotypes for salt tolerance. However, many plant breeding programs are not equipped to conduct such trials. We tested a simple greenhouse method to screen soybean genotypes for salt tolerance by planting in greenhouse flats filled with a sandy soil and watering with salt water. Known chloride tolerant and chloride sensitive cultivars were randomly planted in 30cm x 50cm x 8cm plastic flats without drainage holes filled with sandy soil. At the V4-V5 growth stage, genotypes were alternately watered with tap water and a .05M salt solution made by mixing table salt an deionized water. Approximately 10-14d after watering with salt water was initiated, the salt sensitive genotypes were dead or showed severe injury while the salt tolerant genotypes showed little or no injury. This method shows promise as a method of differentiating soybean genotypes for salt tolerance.
Speaker Information: J. Grover Shannon, Univ. of Missouri, Univerisity of MO Delta Center P O Box 160, Portageville, MO 63873-0160; Phone: 573-379-5431; E-mail: [email protected]
Session Information: Wednesday, November 5, 2003, 4:00 PM-6:00 PM Presentation Start: 4:00 PM (Poster Board Number: 1030)
