An Improved Method Using an Internal Standard for the Determination of Organochlorine Pesticides in Wildlife.
(A05-stahl296364-poster)
Authors:
R.S. Stahl* - USDA/APHIS/WS/NWRC R. Mauldin - USDA/APHIS/WS/NWRC J.J. Johnston - USDA/APHIS/WS/NWRC
Abstract:
Remediation efforts at a site near Denver CO require that the wildlife on site be monitored for a suite of 10 organochlorine pesticides. We developed a gas chromatography (GC) method using electron capture detectors (ECD) to quantify these pesticides in wildlife tissues, blood and plasma. The method uses single point calibration during daily analysis to confirm that the instrument is in calibration. This daily calibration standard is matched to a previously generated standard curve. To improve the ruggedness of our method we evaluated; pentachloronitrobenzene, 1-bromo-2-nitrobenzene, decachlorobiphenyl, and 2,4,5,6-tetrachloro-m-xylene for use as internal standards. Data collected during the comparison of the four compounds are presented. Under the conditions established for our GC method,
2,4,5,6-tetrachloro-m-xylene was the best candidate for use as an internal standard. The internal standard has greatly improved the ruggedness of the method, allowing us to meet our calibration requirements on successive days.
Speaker Information: Randal Stahl, USDA/APHIS/WS/NWRC, NWRC, Fort Collins, CO 80521;
Phone: (970)266-6062; E-mail: [email protected]
Session Information: Monday, November 3, 2003, 4:00 PM-6:00 PM Presentation Start: 4:00 PM (Poster Board Number: 219)