QTL Mapping of Gray Leaf Spot Resistance in Ryegrass and Comparison with Blast Resistance Genes in Rice.
(C05-curley614056-oral)
Authors:
J. Curley* - Univ. of Wisconsin S.C. Sim - Univ. of Wisconsin
S. Warnke - US National Arboretum, Washington, D.C.
R. Barker - USDA-ARS, Corvallis, OR S. Leong - USDA-ARS, Madison, WI G. Jung - Univ. of Wisconsin
Abstract:
Gray leaf spot (GLS) is a serious fungal disease on the important turf and forage species, perennial ryegrass (Lolium perenne) caused by the rice blast fungus Magnaporthe grisea. Early reports suggest little resistance is present in perennial ryegrass cultivars. However, greenhouse inoculations in our lab using one ryegrass isolate suggests some resistance is segregating in an annual x perennial ryegrass (MFA x MFB) pseudo-F2 population. A well-saturated genetic linkage map has been constructed for this population using RFLP, RAPD, AFLP, and SSR markers, and the segregation seen allows quantitative trait locus (QTL) analysis in this population. Potential QTL for resistance have been detected on at least three linkage groups. To confirm that QTL detected in the current population are still detected in the next generation, several resistant segregants have been crossed with susceptible perennial plants, from a different genetic background, to form a new pseudo-F2 mapping population.
Locations and effects of QTL for GLS resistance can be compared between rice and ryegrass via comparative QTL mapping, which can then lead to map-based cloning of novel resistance genes as well as marker-assisted breeding for gray leaf spot resistance in perennial ryegrass.
Speaker Information: Joseph Curley, Univ. of Wisconsin, 1630 Linden Drive, Madison, WI 53706;
Phone: (608)262-0061; E-mail: [email protected]
Session Information: Tuesday, November 4, 2003, 7:30 AM-11:25 AM Presentation Start: 8:05 AM
Keywords: Ryegrass; Gray leaf spot; Resistance; QTL comparisons
