Origins of Allelic Diversity and Genic Regions revealed by Microsatellite EST Markers in Cotton. (5896)
Authors:
M. Ulloa* - USDA-ARS, Shafter, CA Y. Park - USDA-ARS, Shafter, CA M.S. Alabady - UC-DAVIS, CA M. Stewart - Univ. of Arkansas T. Wilkins - UC-DAVIS, CA
Abstract:
EST microsatellites were used to elucidate origin of allelic diversity in tetraploid cottons. PCR amplification of microsatellites gave 562 polymorphic and 458
monomorphic DNA fragments among six species. Polymorphic amplicons revealed five scenarios of transferability and rearrangement of amplicons for the
allotetraploid AD cottons (G. hirsutum and G. barbadense) compared to the
progenitor diploid genomes A-(G. herbaceum and G. arboreum) and D-(G. thurberi and G. harknessii). Approximately 8.5% of the microsatellites yielded DNA
amplicons from the diploid genomes that matched in size those amplified from both tetraploids; 7.6% gave amplicons from the diploid A or D genomes that were absent in the AD genomes; 45.8% amplified at least one novel amplicon in the AD
genomes that were absent in the A or D genomes; and 30.4% amplified amplicons in AD genomes that matched alleles in the diploids but without specific synteny.
Partial results indicate that tetraploid cottons underwent a higher rate than expected of DNA rearrangement at the gene level after allopolyploidization. PCR-stable amplicons were 50%, including monomorphic microsatellites. Selected
microsatellite markers will be used to evaluate additional diploid and tetraploid species. Genetic relationships among species will be depicted from dendograms constructed from loci-genic sequence information and PCR-DNA amplicons.
Speaker Information: Mauricio Ulloa, USDA-ARS, Shafter, CA, 17053 N. Shafter Ave., Shafter, CA 93263; Phone: 661-746-8009; E-mail: [email protected]
Session Information: Wednesday, November 3, 2004, 4:00 PM-6:00 PM Presentation Start: 4:00 PM (Poster Board Number: 1184)
Keywords: Cotton; Polyploidization; microsatellites; Phylogenetics