Comparative proteome analysis between C . briggsae embryos and larvae reveals a role of chromatin modification proteins in embryonic cell division
Item Type Article
Authors An, Xiaomeng;Shao, Jiaofang;Zhang, Huoming;Ren, Xiaoliang;Ho, Vincy Wing Sze;Li, Runsheng;Wong, Ming-Kin;Zhao, Zhongying Citation An X, Shao J, Zhang H, Ren X, Ho VWS, et al. (2017) Comparative
proteome analysis between C . briggsae embryos and larvae reveals a role of chromatin modification proteins in embryonic cell division. Scientific Reports 7. Available: http://dx.doi.org/10.1038/
s41598-017-04533-8.
Eprint version Publisher's Version/PDF
DOI 10.1038/s41598-017-04533-8
Publisher Springer Nature
Journal Scientific Reports
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Link to Item http://hdl.handle.net/10754/625145
Comparative proteome analysis between C. briggsae embryos and larvae reveals a role of chromatin modification proteins in embryonic cell division
Xiaomeng An†#1, Jiaofang Shao†#2, Huoming Zhang‡, Xiaoliang Ren†, Vincy Wing Sze Ho†, Runsheng Li†, Ming-Kin Wong†, Zhongying Zhao†*
†Department of Biology, Hong Kong Baptist University, Hong Kong, China; ‡Biosciences Core Laboratory, King Abdullah University of Science and Technology, 23955-6900 Thuwal, Saudi Arabia.
1Current address: Core Laboratory, The University of Hong Kong-Shenzhen Hospital, Shenzhen, People’s Republic of China; 2Current address: Department of Bioinformatics, School of Basic Medical University, Nanjing Medical University, Nanjing, People’s Republic of China
Table S1. List of primer sequences used for PCR amplification of dsRNA template.
Forward Sequence Reverse Sequence
cbr-lin-53 TAATACGACTCACTATAGGGacgaccagactgtatgtcactggga TAATACGACTCACTATAGGGttcgtcgcgatgagattcgaacgaa cbr-swsn-6 TAATACGACTCACTATAGGGttccagaagtcactccgagctacga TAATACGACTCACTATAGGGgcaccgtattttcgttcggcttgag cbr-let-418 TAATACGACTCACTATAGGGtaatggcgacccgttgaactctgac TAATACGACTCACTATAGGGcggctgcagtttcggtattctcaga cbr-lin-40 TAATACGACTCACTATAGGGcgaagatgcattggacaagtgtggc TAATACGACTCACTATAGGGcagaatttcctgctgcagcctgttg cbr-isw-1 TAATACGACTCACTATAGGGcgtgctggaggtcttggaatcaact TAATACGACTCACTATAGGGtttccggctctcattgcttccttgt cbr-smo-1 TAATACGACTCACTATAGGGagtaacgaggtccacttccgagtca TAATACGACTCACTATAGGGgtaggggtgacgacggtaaaacagg cbr-sip-1 TAATACGACTCACTATAGGGtgtccctcgatggtgagaatgttgc TAATACGACTCACTATAGGGcgcggtcttccagaattgtctcgat
Table S2. List of identified proteins in all replicates by iTRAQ assay.
Table S3. List of the C. briggsae proteins specifically identified by iTRAQ compared to those by SILAC.
Table S4. List of identified DEPs in all samples.
Table S5. List of GO and KOG categories for the 247 differentially expressed proteins (DEPs) identified by iTRAQ between embryo and two larval stages (L1 and L4).
Fig. S1. Full cell lineage trees for embryos of wildtype and RNAi against five embryonically up- regulated genes. See also Fig 6 B-E.