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Kingdom of Saudi Arabia National Commission for Academic Accreditation &

Assessment

ﺔــﻳﺩﻮﻌﺴﻟﺍ ﺔــﻴﺑﺮﻌﻟﺍ ﺔــﻜﻠﻤﻤﻟﺍ ﻢــﻳﻮـﻘﺘﻠﻟ ﺔــــﻴﻨﻁﻮﻟﺍ ﺔــــﺌﻴﻬﻟﺍ ﻲــــﻤــﻳﺩﺎــﻛﻷﺍ ﺩﺎـــﻤــﺘـــﻋﻻﺍﻭ

Kingdom of Saudi Arabia

The National Commission for Academic Accreditation & Assessment

COURSE SPECIFICATION

Microbial Physiology 4013452-3

Revised September 2015

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Kingdom of Saudi Arabia National Commission for Academic Accreditation &

Assessment

ﺔــﻳﺩﻮﻌﺴﻟﺍ ﺔــﻴﺑﺮﻌﻟﺍ ﺔــﻜﻠﻤﻤﻟﺍ ﻢــﻳﻮـﻘﺘﻠﻟ ﺔــــﻴﻨﻁﻮﻟﺍ ﺔــــﺌﻴﻬﻟﺍ ﻲــــﻤــﻳﺩﺎــﻛﻷﺍ ﺩﺎـــﻤــﺘـــﻋﻻﺍﻭ

Course Specification

For Guidance on the completion of this template, please refer to of Handbook 2 Internal Quality Assurance Arrangements

Institution: UM AL – QURA UNIVERSITY

College/Department : Faculty of Applied Science – Department of Biology

A Course Identification and General Information

1. Course title Microbial Physiology 2. Course code: 4013452-3

2. Credit hours: 3hrs

3. Program(s) in which the course is offered. : BSc Microbiology 3. Name of faculty member responsible for the course:

Dr. Abdelrahman Assaeedi ([email protected]) 5. Level/year at which this course is offered: 3rd Year / Level 6

6. Pre-requisites for this course (if any): Biochemistry (4012312-3) / Introductory Microbiology (4012401-4)

7. Co-requisites for this course (if any): --- 8. Location if not on main campus: Main campus

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Kingdom of Saudi Arabia National Commission for Academic Accreditation &

Assessment

ﺔــﻳﺩﻮﻌﺴﻟﺍ ﺔــﻴﺑﺮﻌﻟﺍ ﺔــﻜﻠﻤﻤﻟﺍ ﻢــﻳﻮـﻘﺘﻠﻟ ﺔــــﻴﻨﻁﻮﻟﺍ ﺔــــﺌﻴﻬﻟﺍ ﻲــــﻤــﻳﺩﺎــﻛﻷﺍ ﺩﺎـــﻤــﺘـــﻋﻻﺍﻭ

B Objectives

After completing this course student should be able to:

1. Distinguish between the eukaryotic and prokaryotic cell structure.

2. Describe the bacterial cell components and its function role in the cell 2. Define basic concepts of microbial physiology.

3. Explain microbial growth, growth kinetics and factors affecting growth.

4. Evaluate the importance of central pathways off carbohydrate metabolism for microbial physiology.

5.Cell enzymes and its role in nutrition

5. Illustrate macromolecular synthesis and processing.

6. Link the microbial physiology to the genomics of cells.

C. Course Description (Note: General description in the form to be used for the Bulletin or Handbook should be attached):

This course is designed for students of applied microbiology to cover the basic aspects of microbial physiology. To explain prokaryotic and eukaryotic structure and composition as well as the means by which nutrients are transported into cells across membranes. The important metabolic processes that occur in prokaryotes and eukaryotic microorganisms under different environmental conditions will be discussed. Explain the central metabolic pathways starting from glucose, as well as other trophic variations found in prokaryotes including the use of organic compounds other than glucose, anaerobic fermentation, anaerobic respiration, chemolithotrophy and photosynthesis. Kinetic of the energy and biochemistry of Nitrogen fixation also will be covered. The regulation of metabolism through control of gene expression and enzyme activity is also covered. Finally, development of the laboratory skills for students will be targeted.

1 Topics to be Covered

Topic No of

Weeks

Contact hours

Introduction:

-Overview of microbial physiology

-Microbial diversity and relation to activity

1 2

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Kingdom of Saudi Arabia National Commission for Academic Accreditation &

Assessment

ﺔــﻳﺩﻮﻌﺴﻟﺍ ﺔــﻴﺑﺮﻌﻟﺍ ﺔــﻜﻠﻤﻤﻟﺍ ﻢــﻳﻮـﻘﺘﻠﻟ ﺔــــﻴﻨﻁﻮﻟﺍ ﺔــــﺌﻴﻬﻟﺍ ﻲــــﻤــﻳﺩﺎــﻛﻷﺍ ﺩﺎـــﻤــﺘـــﻋﻻﺍﻭ

Microbial growth

- Growth kinetics, growth curve, measurement of growth and growth yields.

- Synchronous growth and Continuous culture.

Factors affecting microbial growth.

2 4

Metabolic diversity among microorganisms

Respiration, Photosynthesis, acetogenesis, methanogenesis, nitrogen fixation, and hydrocarbon transformation in microorganisms.

2 2

Central pathways and microbial activities

- Carbohydrates: major pathways (EMP, TCA, Glyoxylate cycle).

Biosynthesis of Oligosaccharides.

Lipids: Fatty acid biosynthesis and oxidation

4 8

Aerobic vs anaerobic processes

- Respiratory chains (components and function)

- Aerobic vs anaerobic respiration and relation to growth - Growth of anaerobes

Fermentation and fermentative microorganisms

2 4

Biocatalysis and Biocatalyst

-Enzymes and their classification, Enzyme kinetics, allosteric enzymes,

-Michaelis- Menten equation, - coenzyme, isozyme,

-Enzyme inhibition andregulation.

-Enzyme structure and function.

-Factors affecting enzyme activity .

3 6

14 weeks

28hrs

3B2 Course components (total contact hours per semester):

4BLecture : 28 5BTutorial: 6BPractical: 42 7BOther:

8B3. Additional private study/learning hours expected for students per week. (This should be an average :for the semester not a specific requirement in each week):

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Kingdom of Saudi Arabia National Commission for Academic Accreditation &

Assessment

ﺔــﻳﺩﻮﻌﺴﻟﺍ ﺔــﻴﺑﺮﻌﻟﺍ ﺔــﻜﻠﻤﻤﻟﺍ ﻢــﻳﻮـﻘﺘﻠﻟ ﺔــــﻴﻨﻁﻮﻟﺍ ﺔــــﺌﻴﻬﻟﺍ ﻲــــﻤــﻳﺩﺎــﻛﻷﺍ ﺩﺎـــﻤــﺘـــﻋﻻﺍﻭ

12h (reports & essay)

4. Development of Learning Outcomes in Domains of Learning

9BFor each of the domains of learning shown below indicate:

10BA brief summary of the knowledge or skill the course is intended to develop;

11BA description of the teaching strategies to be used in the course to develop that knowledge or skill;

12BThe methods of student assessment to be used in the course to evaluate learning outcomes in the domain concerned.

a. Knowledge : Description of the knowledge to be acquired

Upon successful completion of this course The student will be able to:

1. Define basic concepts of microbial physiology.

2. Distinguish between the eukaryotic and prokaryotic cell structure and function.

3. Explain microbial growth, growth.

4. Define types of membrane transport for nutrient uptake and protein excretion.

5. Describe the bacterial cell components and its function role in the cell 6. 3. Explain microbial growth, growth kinetics and factors affecting growth

13B(ii) Teaching strategies to be used to develop that knowledge

• The methodology includes a combination of lectures by the lecturer, seminar presentation by the students and web-interactions.

• At the end of the programme, students will be divided into groups for seminar presentation on important areas of the course to assess their understanding and comprehension of the course.

• All students will be involved in on-line learning process and each student is required to create an E-mail address to facilitate student web interactions.

• Using images and movies

• Encouraging students to collect the new information about what the new in microbial physiology

• Enable the reference books and scientific sites concerning bacteriology in

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Kingdom of Saudi Arabia National Commission for Academic Accreditation &

Assessment

ﺔــﻳﺩﻮﻌﺴﻟﺍ ﺔــﻴﺑﺮﻌﻟﺍ ﺔــﻜﻠﻤﻤﻟﺍ ﻢــﻳﻮـﻘﺘﻠﻟ ﺔــــﻴﻨﻁﻮﻟﺍ ﺔــــﺌﻴﻬﻟﺍ ﻲــــﻤــﻳﺩﺎــﻛﻷﺍ ﺩﺎـــﻤــﺘـــﻋﻻﺍﻭ

internet.

(iii) Methods of assessment of knowledge acquired:

• Periodical exam and reports 10%

• Mid- term theoretical exam 20%

• Mid-term practical exam 5%

• Final practical exam 15%

• Final exam 50%

14Bb. Cognitive Skills

15B(i) Cognitive skills to be developed

Having successfully completed the course students should be able to:

1. Evaluate the importance of central pathways of carbohydrate metabolism for microbial physiology

2. Link the microbial physiology to the genomics of cells.

3. Explain how some microorganisms can degrade the macromolecule polymers

4. Calculate the kinetic energy in aerobic and anaerobic microbes

16B(ii) Teaching strategies to be used to develop these cognitive skills:

0B- Lectures

1B-Brain storming

2B-Discussion

17B(iii) Methods of assessment of students cognitive skills

18B- Exam must contain questions that can measure these skills.

19B- Quiz and exams

20BDiscussions after the lecture

21Bc. Interpersonal Skills and Responsibility

At the end of the course, the student will be able to:

-Analyze the growth kinetic data.

Illustrate macromolecular synthesis and processing.

(i) 22BTeaching strategies to be used to develop these skills and abilities - Lab work

- Case Study - Active learning

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Kingdom of Saudi Arabia National Commission for Academic Accreditation &

Assessment

ﺔــﻳﺩﻮﻌﺴﻟﺍ ﺔــﻴﺑﺮﻌﻟﺍ ﺔــﻜﻠﻤﻤﻟﺍ ﻢــﻳﻮـﻘﺘﻠﻟ ﺔــــﻴﻨﻁﻮﻟﺍ ﺔــــﺌﻴﻬﻟﺍ ﻲــــﻤــﻳﺩﺎــﻛﻷﺍ ﺩﺎـــﻤــﺘـــﻋﻻﺍﻭ

- Small group discussion

23B(iii) Methods for assessment of the students interpersonal skills and capacity to carry responsibility

• Evaluate the efforts of each student in preparing the report.

• Evaluate the scientific values of reports.

• Evaluate the work in team

• Evaluation of the role of each student in lab group assignment

• Evaluation of students presentations

24Bd. Communication, Information Technology and Numerical Skills (i) 25BDescription of the skills to be developed in this domain.

26BAt the end of the course, the student will be able to:

• Enhancing the ability of students to use computers and internet.

• Interpret microbiological data

Calculate enzymes activities

Know how to write a report.

(ii) 27BTeaching strategies to be used to develop these skills

Homework (preparing a report on some topics related to the course depending on web sites).

Seminarspresentation

Practical during carryout the experiments in the lab.

28B(iii) Methods of assessment of students numerical and communication skills - Evaluation of presentations

- Evaluation of reports - Practical exam

29Be. Psychomotor Skills (if applicable)

At the end of the course, the student will be able to:

- Carry out the Experiments to study the factors affecting enzymes and their activities

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Kingdom of Saudi Arabia National Commission for Academic Accreditation &

Assessment

ﺔــﻳﺩﻮﻌﺴﻟﺍ ﺔــﻴﺑﺮﻌﻟﺍ ﺔــﻜﻠﻤﻤﻟﺍ ﻢــﻳﻮـﻘﺘﻠﻟ ﺔــــﻴﻨﻁﻮﻟﺍ ﺔــــﺌﻴﻬﻟﺍ ﻲــــﻤــﻳﺩﺎــﻛﻷﺍ ﺩﺎـــﻤــﺘـــﻋﻻﺍﻭ

30B(ii) Teaching strategies to be used to develop these skills

- Follow up students the students in lab and during carryout all the laboratory experiments

(iii)31BMethods of assessment of students psychomotor skills

• Giving additional marks for the students they have accurate laboratory results and good seminar presentation

Practical exam.

5. Schedule of Assessment Tasks for Students During the Semester Assessment task

(e.g. essay, test, group project, examination, speech, oral presentation, etc.)

Week Due Proportion of Total Assessment

1 Exercises & Home works All weeks 5 %

2 Participation All weeks 5 %

3 Written Test (1) 6th week 15%

4 Written Test (2) 11th week 15%

5 Final Exam (Practical) 15th week 20%

6 Final Exam (theoretical) 16th week 40%

D. Student Support

1. Arrangements for availability of faculty for individual student consultations and academic advice. (include amount of time faculty are available each week) Office hours: 10 hrs

E. Learning Resources Required Text(s):

1- Book note prepared by Dr. Abdelrahman Assaedii

2- Madigan, M.T; Martinko, J.M and Parker, J (2012). Brocks Biology of Microorganisms. (13th ed.). Prentice Hall International.ISBN-13: 978-0-321-64963-8 ---

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Kingdom of Saudi Arabia National Commission for Academic Accreditation &

Assessment

ﺔــﻳﺩﻮﻌﺴﻟﺍ ﺔــﻴﺑﺮﻌﻟﺍ ﺔــﻜﻠﻤﻤﻟﺍ ﻢــﻳﻮـﻘﺘﻠﻟ ﺔــــﻴﻨﻁﻮﻟﺍ ﺔــــﺌﻴﻬﻟﺍ ﻲــــﻤــﻳﺩﺎــﻛﻷﺍ ﺩﺎـــﻤــﺘـــﻋﻻﺍﻭ

Recommended Reading List

1-Moat, A.G; Foster, J.W and Spector, M.P (2002). Microbial Physiology (4th ed.). John Wiley & Sons Inc.

2- Cappuccino, J.G and Sherman, N (2002). Microbiology, a Laboratory Manual (6th ed.).

Benjamin Cummings

2. Prescott, L., Harley, J. and Klien, D. (2005). Microbiology, MacGraw --- Electronic Materials, Web Sites (eg. Web Sites, Social Media, Blackboard, etc.) -BioCyc Database Collection (http://biocyc.org/)

-ASM-Microb Library (http://www.microbelibrary.org/)

---

Other learning material such as computer-based programs/CD, professional standards/regulations

PPT prepared by Dr. Abdelrahman Asaedii

F. Facilities Required

32BIndicate requirements for the course including size of classrooms and laboratories (ie number of seats in classrooms and laboratories, extent of computer access etc.) 1. Accommodation (Lecture rooms, laboratories, etc.)

• Class room is already provided with data show

• The area of class room is suitable concerning the number of enrolled students (68) and air conditioned.

2. Computing resources

• Providing class rooms with computers and labs with data show.

3.Other resources (specify --eg. If specific laboratory equipment is required, list requirements or attach list)

• Availability of some reference bacterial strains

Availability different specific media and chemicals used for study the factors affects enzymes and microbial growth

G Course Evaluation and Improvement Processes

1. Strategies for Obtaining Student Feedback on Effectiveness of Teaching

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Kingdom of Saudi Arabia National Commission for Academic Accreditation &

Assessment

ﺔــﻳﺩﻮﻌﺴﻟﺍ ﺔــﻴﺑﺮﻌﻟﺍ ﺔــﻜﻠﻤﻤﻟﺍ ﻢــﻳﻮـﻘﺘﻠﻟ ﺔــــﻴﻨﻁﻮﻟﺍ ﺔــــﺌﻴﻬﻟﺍ ﻲــــﻤــﻳﺩﺎــﻛﻷﺍ ﺩﺎـــﻤــﺘـــﻋﻻﺍﻭ

• Questionaries

• Open discussion in the class room at the end of the lectures

2. Other Strategies for Evaluation of Teaching by the Instructor or by the Department

• Revision of student answer paper by another staff member.

• Analysis the grades of students.

3. Processes for Improvement of Teaching

• Preparing the course as PPT.

• Using scientific movies.

• Coupling the theoretical part with laboratory part

Periodical revision of course content.

4. Processes for Verifying Standards of Student Achievement (eg. check marking by an independent faculty member of a sample of student work, periodic

exchange and remarking of a sample of assignments with a faculty member in another institution)

• After the agreement of Department and Faculty administrations

5 Describe the planning arrangements for periodically reviewing course effectiveness and planning for improvement.

• Periodical revision by Quality Assurance Units in the Department and institution

Faculty member responsible for the course:

Faculty member responsible for the course:

Prepared by faculty staff:

1.Dr. Abdulrahman S. Assaeedi 2. Dr. Khaled Elbanna

Signature:

Date Report Completed: September 2015

Revised by: Signature:

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Kingdom of Saudi Arabia National Commission for Academic Accreditation &

Assessment

ﺔــﻳﺩﻮﻌﺴﻟﺍ ﺔــﻴﺑﺮﻌﻟﺍ ﺔــﻜﻠﻤﻤﻟﺍ ﻢــﻳﻮـﻘﺘﻠﻟ ﺔــــﻴﻨﻁﻮﻟﺍ ﺔــــﺌﻴﻬﻟﺍ ﻲــــﻤــﻳﺩﺎــﻛﻷﺍ ﺩﺎـــﻤــﺘـــﻋﻻﺍﻭ

Signature

ﺕﺎﻘﻓﺮﻣ : ﺔﻴﺋﺎﻬﻨﻟﺍﻭ ﺔﻴﻔﺼﻨﻟﺍﻭ ﺔﻳﺭﻭﺪﻟﺍ ﺕﺍﺭﺎﺒﺘﺧﻻﺍ ﻦﻣ ﺝﺫﺎﻤﻧ1. Dr. Khaled Elbanna

2. Dr. Hussein H. Abulreesh 3. Dr. Shady Elshahawy

Date: 1.10.2015 Program Chair

Dr. Hussein H. Abulreesh

Signature:

Dean

Prof. Samir Natto

Signature:

Date:

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