Engineering resistance against Tomato yellow leaf curl virus via the CRISPR/Cas9 system in tomato
Item Type Article
Authors Tashkandi, Manal;Ali, Zahir;Aljedaani, Fatimah R.;Shami, Ashwag;Mahfouz, Magdy M.
Citation Tashkandi M, Ali Z, Aljedaani F, Shami A, Mahfouz MM (2018) Engineering resistance against Tomato yellow leaf curl virus via the CRISPR/Cas9 system in tomato. Plant Signaling & Behavior 13: e1525996. Available: http://
dx.doi.org/10.1080/15592324.2018.1525996.
Eprint version Post-print
DOI 10.1080/15592324.2018.1525996
Publisher Informa UK Limited
Journal Plant Signaling & Behavior
Rights Archived with thanks to Plant Signaling & Behavior Download date 2023-10-31 13:31:19
Link to Item http://hdl.handle.net/10754/626438
Supplementary Figure 1. Confirmation of Cas9 expression in N. benthamiana
Western blot confirmation of Cas9 expression in three individual T3 permanent lines (for targeting IR, CP and Rep) of N.
benthamiana. Anti-flag anti-body was used to detect flag-tagged Cas9. Arrow indicates the expected 169 kDa Cas9.
Supplementary Figure 2. Confirmation of Cas9 expression in S. lycopersicum
Western blot confirmation of Cas9 expression in six individual permanent lines (for targeting CP and Rep) of (S.
lycopersicum). Anti-flag anti-body was used to detect flag-tagged Cas9. Arrow indicated the expected 169 kDa Cas9.
Supplementary Figure 3. RCA for TYLCV genome accumulation
RCA assay for accumulation of the TYLCV genome in T3 lines of tomato expressing CRISPR-Cas9. Compared to the wild type (set as 100%), plants expressing CRISPR-Cas9 accumulate a lower level of the TYLCV genome.
Supplementary Figure 4. Semi-quantitative PCR for TYLCV2.3 genome accumulation
Semi-quantitative PCR for TYLCV genome accumulation in T3 lines of tomato expressing CRISPR-Cas9. Compared to the wild type (set as 100%), plants expressing CRISPR-Cas9 accumulate a reduced level of the TYLCV genome.
Supplementary Figure 1. Cas9 expression confirmation in N. benthamiana
Western blot confirmation of Cas9 expression in three individual T3 permanent lines (for targeting IR, CP and Rep) ofN. benthamiana. Anti-flag anti-body was used to detect flag-taged Cas9. Arrow indicated the expected 169kda Cas9.
Supplementary Figure 2. Cas9 expression confirmation in S.lycopersicum
Cas9-flag
Cas9-flag
Western blot confirmation of Cas9 expression in six individual permanent lines (for targeting CP and Rep) of (S. lycopersicum). Anti-flag anti-body was used to detect flag-taged Cas9. Arrow indicated the expected 169kda Cas9.
Supplementary Figure 3. RCA for TYLCV genome accumulation
RCA assay for the TYLCV genome accumulation in T3 lines of tomato expressing CRISPR-Cas9. Compared to the 100% wild type, plants expressing CRISPR-Cas9 accumulate a reduce level of TYLCV genome.
4kb3kb 2kb 1.6kb
TYLCV Nb.PDS
amplicon % 48 28 100 34 31 100
Supplementary Figure 4. semi-quantitative PCR for TYLCV2.3 genome accumulation
Simi-quantitative PCR for the TYLCV genome accumulation in T3 lines of tomato expressing CRISPR-Cas9. Compared to the 100% wild type, plants expressing CRISPR-Cas9 accumulate a reduce level of TYLCV genome.