KHOA HQC C O N O NOHt
i\iGHi£i\i Ciiu SIJ Bi£iv DOIVG H A M LUOIMG P H Y T I C AXIT TRDIMG HAT TIJ^ CAC
T H £ D D T BI^IMLUA DUOC
E A Y T A O B A I V G C H I £ U X A T I A B A M A I V I
Nguyfo Th( Lang', Ph?ni Th) Bi Tu', Ph?m VSn tit^, Bili Chi Bftu' T6MTAT
Phytic axit c6 ciu tgo phfln tu \i myoinositol 1,2,3,4,5,6 hexaltis dihydro ph6t phM (Ins P6), la thanh phin chinh trong ngu6n ph(St pho (P) di; trtl ciia thi;c yjt cUim khofing 50-80% phdt pho t6ng ciia h^t (Lott, 1984). O pH sinh ly phytic axit b d^ng da ion tich di^n Am k^m gli^ ch^t ciic khoiing dinh dirOng t^o phijc chiit kh6 ti^u. Hon niia, phdt pho CT dang phytic hay phytate do con ngirW v4 dOng v$t c6 c^u tao da dfly don khdng th^ h^p thu duoc va khi bfli th^ ra ngoAi gSy 6 nhiJn m61 tni6ng. Nghiftn cifu chon tao gl6ng Itia c6 him luong phytic axit thSp U r^t c6 y nghia. Nam gidng lua: OM819, OM4900, OM3536, D4 vS D8 dugc chiiu x? b4ng Ua gamma a 5 li^u luong: 10,20, 30, 40 vi 50kr ( Krad ) nh^m t^o d^t bi^n c6 him luong phytic axit th^p. K^t qui cho th^y, 6 Wu chiiu xa lOkr chua xuSt hi^n th^ d«t bi^n c6 h?t bi^u hi*n phytic axit thSp. 6 liiu chiiu xa 20kr c6 3 qu4n th^ OM819, OM4900 va OM3536 v6i 8 cSy c6 h9t bi^u hi^n phytic axit thip. 6 liiu 30kr, xuSt hi^n 7 c4y c6 hat bi^u hi^n phytic axit UiSp ttiuOc 4 quSn th* OM819, OM4900, OM3536 vi D4. 6 li«u 40kr, 4 qu4n th* trtn chi xuSt hi*n 5 cSy bi*u hijn phytic axit tiifip, v6i 3 cay bi*u hi^n dp 3 vi 2 cay bi*u hi^n c^p 4. 6 h*u chi*u xa 50kr chi 1 cSy xu^t hi$n phytic axit 6 miic 3 thuOc quin tii* OM819.
Tu khoa: Phytic axit thSp. lua d^t bi€'n. da gamma
LBiTVANK
Phan Ion cac gidng lua gao hidn nay thuong c6 ham lirong phytic axit cao va rat ngheo duOng khoang vi luong (sat, kem...). Thieu sat se dan d^n tinh trang tliieu mau, hi&n tirong nay xay ra o p h ^ Ion tre em va phu nu trong giai doan mang thai (Gillespie, 1998). Bin canh sat, thiSu kem lam cho co Ih^ suy yixi, b^nh tat gay anh huong Ain sir sinh truong va phit triSn ctia co th^, th$m chi c6 t h i gSy ttr vong (Bhutta ZA et al, 1999). M}t kh4c, ngu6n ph6t pho dtr tru chinh trong hgt du6i d?ing phytic axit, phytate thdng thuong chiim tir 1 d^n vai p h i i tram khoi lu<;mg kh6 cua hat, v^ trong vai tniimg hpp no chiim khoang 50 - 80% phcit pho tdng ciia h?t (Lott, 1984). Tuy nhiSn, lui?ng phot pho tir cdc hop chit tren (mudi phytate) con nguoi v4 d^ng vjt d?
day don Iji khdng t h i hSp thu dupe vi thiiu men tiSu hoa. C^c chat n^y dupe thai ra ngoai theo phSn, day dupe xem 14 mdt trong nhting ngudn gjy 6 nhiSm phot pho.
'Vifn lua DBSCL
'Trung tam Khuyen ndng TP Can tho 'Vi?n KHKT Nong nghi?p mien Nam
Viec nghien ciiu tao dupe gidng liia co ham lupng phytic axit thap dupe cac nha chpn gidng quan tam, nham giai quyit cac van d i lien quan din siic khoe con nguoi, dinh duOng dpng vat va quan ly dinh duOng sin p h i m nong nghiip. D i tai "Banh gia sa bi^n ddng liiun lupng Phytic axit trong hat ctia cac ddng Ilia dupe xu ly dpt bi& bang chiAi xa Ha gamma (yjf dupe thi,ic hiin, nham xac djnh miic d$
biin dpng ham lupng phytic axit cua cac dong liia sau chiiu xg tr^ng a giai do^n M2 so voi gidng g&
ban ddu. T r i n co so do, chpn Ipc cac dong lua dot bien c6 ham lupng phytic axit thip bd sung vao ngudn v§t liiu ban dau cung nhu phuc vy cho muc Ueu t?o chpn gidng liia theo huong moi vi phSm c h i t
LV/lTlIU MPMnNGPHiiPIUnCni l . V J t l i i u
- Gidng Ilia dupe su dung tao ddng dot biin:
OM819, OM3536, OM4900, D4 va D8.
- Gidng Ilia ddi chiing c6 h i m lupng phytic axit thip: Wild rice (gidng lila My).
T i c nhan gSy dot biin: Ly hpc - tia gamma {ngudn Cd^.
10 NONG NGHIfP VA PHAT TRIEN N 6 N G THON - KY 2 - THANG 5/2012
KHOA HOC C 6 N G N G H £
- Dung dich HCl, HaSO^, (NHiJjMo, Vitamin C, KHjPO^, dung dich Chen'S (1956) v^ mOt so thi^t h\
phu trg cho vi6c lay chi tiSu nfing hpc v^ phan tich HfR
Thi nghiem dugc thuc hi^n tai Vi^n Lua DBSCL, thu6c xaTan Thanh, huySn Th6i Lai, T h ^ h ph6 Can Tha. b^t dau tii thang 5/2009.
2. Phuong ph^p nghifen cihi
a. Phuong phap gay tao dongddtbien &M2 Uiy ngSu nhi6n lOg hat liia kho cho m6i mSu dem chieu xa bang tia gamma (nguon Co™) v6i 5 li^u luong: 10, 20, 30,40 va 50Kr tai Vi^n nghien cuu Hat nhan Da Lat. M5u liia sau chieu xa duoc trong qua 2 VU (the he thii 2 - M2), v6i c^ch bd tri thi nghiem theo the thiic khoi hoan toan ngau nhi^n 3 Idn lap lai.
Cac ca th^ co cung mpt lieu lugng chieu xa dupe cay 1 tep voi khoang each 20x15 cm ( h ^ g x cay).
b. Phuong phap danh gia ham luong phytic axit thap
Ky thuat phan lap dot bien phytic axit th^p tr^n nhi^u ioai cay tr6ng khac nhau dupe thuc hidn gian tiep thong qua phan tich lupng phot pho v6 co cao (HIP) dupe mo ta bdi Wilcox etal. (2000) va Larson etal. (2000). Cac cay liia sau chi^u xa dupe phan tich theo phuong phap tach chi^t 1 hat cua Chen et al.
(1956). Ddu ti^n, lay m6i cay 8 hat, nghien rifing timg hat va cho vao hing gi^ng tren dla vi do chuan 96 gi^ng (96-well), ti^p tuc them vao m6i gi^ng 200pl 0,4M HCI va tru mhx qua d§m a nhi^t d6 ph6ng.
Tiep theo, lay 10 pi dung dich mau da u qua d&m cho vao gieng ciia vi dp mdi theo thu tu, thfim ti^p 90 pi nuoc cat, d6ng thoi ti^p tuc cho vao mdi gi^ng 100 pi dung dich Chen'S, de mau a nhi^t do phong khoang 45 phiit Ox6i cimg, xac dinh ham lupng ph6t pho v6 CO ^hs^tic axit) cua h?it thong qua mau (mdu xanh) bieu hi6n cua san pham phan tich so voi bang mau chuan dupe thi^t k^ theo Chen et al. (1956). Nhung mau CO mau xanh tuong ung vdi chuan miic 3 cua bang mau c h u & trd I6n dupe danh gia co ham lupng phot pho v6 CO (P) cao hay hjim lirong phytic axit th^p.
•.KETQUAVATHAOLUAN
1. Sv bi^n dOng h i m lupng phytic axit trfin qudn th^ OM819 sau chi^u x?
K^t qua phan tich HIP ciia 100 cay thupc quan th^ OM819 d M2 da qua chi^u xa so vdi gidng gdc cho th^y, ham lupng phot pho v6 ca (P) hay ham lupng phytic axit hong hat cua qulji th^ sau chieu xa da khac bi$t vdi gidng gdc (hinh 1).
- d li^u lupng chi^u xa lOkr, q u ^ thi chua xuat hi^n cay c6 hat bilu hi^n cap P h-6n miic 3, da sd hat bilu hi^n lupng phytic axit d cap 1 (hinh 1 ) . Sd cay c6 8 hat t h i hi^n dong nhtft mau c^p 1 vi ham lupng phytic axit chidm 65% tdng mau phan tich, con lai la nhung ddng c6 hat bilu hifin phytic axit cap 2.
- O Mbx lupng chidu xa 20kr, phan Ion cac cay da phan tich CO hat bilu hi^n c6 ham lupng phytic axit tr^n cap 1, dac biet 3 dong co hat dat cap P tu 34, lan lupt la: OM819-21, OM81&-31, OM819-35. Xet v^ tdng sd hat dupe phan tich (160 hat) cua quan the, sd hat bilu hi^n P cap 4 chi cdl hat chilm 0,63%, 2 hat bilu hien cap 3 chiem 2,5%, hat bieu hi&n cap 2 chiem 41,88%, va 55% hat bilu hien cap 1 (hinhl). Mat khac, tu 20 cay ciia q u ^ the da danh gia, sd cay bieu hi^n ddng nhat ve mau tren 8 hat phan tich rat it chi cd 2 cay, con lai la nhimg cay cd hat bidu hi^n mau khac nhau thi hien kilu hinh di hpp hi.
- O li^u lupng chidu xa 30kr, sd hat bilu hien d cap 1 chidm 46,25%, h?t bieu hien cap 4 chiem 0,63%, hat bidu hi^n cap 3 chidm 3,13%, con 1^ la hat bidu hi$n c£[p 2. Xet trln sd ddng quan sat, chi xu^t hi$n 1 dong vdi 1 hat la OM819-46 co bieu hien phytic axit c^p 4 va hai dong cd h^t bilu hi^n cap 3 la OM81^48 vaOM819-55(hinh2).
- O lilu lupng xu ly 40kr, phan Idn hat ciia cac cay t h i hi^n lupng phdt pho v6 co hay phytic axit trong hat d cdp 2, chi cd 2 cay vdi 2 hat thi hi^n d cap 4 ciia 2 dong O M 8 1 9 ^ va OM819-77 chiem 1,88%
tdng sd hat Oiinhl; 2).
- O li^u lupng xu ly hat 50kr, trong 20 dong tii q u ^ thi" da phan tich chi cd dong OM819-97 cd 1 hat t h i hi^n ham lupng phytic axit d cap 3 chilm ty 16 0,63% tdng sd h a t
NONG NGHIEP VA PHAT TRIEN N 6 N G T H 6 N - KY 2 - THANG 5/2012
11
KHOA HOC C 6 N 0 N C H C
OKr lOKr 20Kr 30Kr 40Kr 50Kr Hinh 1: TV \(f hat titf hifii rrciw ril]) |)liril plm iu;i Umi: tmir dp cllK^u xa qii;\n llii^ OMHl!) 2. Sirbicii dong li.ini lirinii; phylK" :ixil IrTn <|u;in thi* OM4900 s:iii chiOu x:i
Kr\ qua i)h;iii licli Iri-n UK) dcm^' Ihuiu' (|n:in llir sau LIIU'U \.I va K'o'ij,' ^'(V cho lli.iy, li;ini hn.m^
phytic ;L\II Uoug h;it biru liiUn 'V uiih- cao n!i:il la 3 cap.
- O lii-u luimg xir ly chieu xa KIkr. chua xual hicn dong CO hat bii'u hien ham Immg phytic axil thaji trong qu;m thi' phim tich, hnl chi bii'u hien (V c:ip 1 v;i 2aiinh3).
- O lieu luimg xir ly 20kr, phiin lim cac dong co hat the hien luong phytic axit o cap 1 va 2, tuy nhien CO 3 dong CO hat bicu hli'n a cap 3, lim knrl lii:
OM4900-24, OM49(X)-32 va OM4900-35 Oiinh 4) Xi'l tren long so hat d,a phim tich ciia qiiiin [he, so h;it bi^u hii-n hani Im/njj phytic axil tiong hai lh:i|) (i uji
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D M H I ' ( I M S I ' i)\]K\' D M X r O M K l ' O M K I '
O M J ' K
U i l i l r -15 -16
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Hinh 2: K.M qua phan llch nil'Ui-n Miol sii (loiii; lliiioc quim llie ()M819 :i) l:i 1 li:il ihieiii 2.:i7'i. Ii.il xiial hien cap 2
•111 :13.7.S%. va (i2..'"ila h.il bleu hien cap I (hinh3).
() lieu xa 3()kr, trong liing so Kit) hat tu f|ii:lnth^
dinn." phim tich, so hat bieu hion o ciip I ciii^m ,^>l.2r)%. ciip 2 la 47..^*. va hat the- hli-n ciip 3 cluvcn2 h;it chiiin l.2."v^,, 0iiiih3; 4 ) thuqc ciic cay cijadong O.Ml'.lillMii. OM 19lKl-.'")2. mill eiiy mot hat.
- I ( hell xa KIkr, li:il hieu hien phytic ixil cap 1 ciileni ril.S,S'Ai timg luoiiK hai phan licli, 47..7Cfl6 hat bleu luen ciip 2 vii 0,G3% l:i hai cap 3 vin 1 hat thuoc (l.mKllM49n()-(Vt aiinh4).
- (1 lieu xa r>(lkr, quan llie co 2 iloiiK OM l'.)00«
va 0X119(10-93 co hai hieu hii-ii ]iliytie axil cap 3, iilunii; chi voi 2 ll:il chiem 1,25% liing so hai phan licli, cac c'iy con lai lial chi bleu hii'n (Vciip 1 vacap2 (hinh 3:1)
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90%
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70%
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- > l ) M - l ' ) 0 t l - 4 ( i - * l ) M 4 ' J n i l - i ; ; - » ^ ) M 4 ' ) ( 111-51 - * i ) M 4 ' ' 0 i l - 6 4
- • l ) M 4 9 ( I O - G - • • P d i u d n -^ W i l d rice
Hinh 3. i y 1$ hat ciJa qu4n th« OM4900 thi hi^n c^p phrft pho or t t o g mtic d^ chi& x?
Htah 4. Kit qui phSn tich HIP tr«n mOt s6 ibag thu«c quSn thi OM4900 NONG NGHI|P VA PHAT TRIEN N 6 N G T H 6 N - ICV' 2 - THANG 5/2011
KHOA HQC C O N G NGHt
3. Si; bi^n ddng h ^ lupng phytic axit tr6n qudn th^ OM3536 sau chi^u xa
Kdt qua phan tich HIP cho thay cd su bidn dOng Idn, da sd hat thd hien lupng phytic axit cao (cc(p 1, cap 2). Tuy nhifin, mot sd cay thudc q u ^ thd cd mdt sd hat bi^u hi^n ham lupng phytic axit th^p.
- 6 lieu xa lOkr, p h ^ ion h&t bi^u hi^n cd him lupng phytic axit cao (c^ip 1), chua tii^y xuitt hi$n cSy cd hat bi^u hi^n c^p P tr6n miic 3 (hinh 5).
- 6 lieu xa 20kr, quan th^ cd 2 c^y cd h^it bi^u hi&n h ^ lupng phdt pho v6 ca cao (c^p 3) hay phytic axit ti-ong hat th^p thudc ckc cky ciia ddng OM3536-29 vk OM-3536- 35. Tuy nhi6n, sd hat tiong quan thd cd ham lupng phytic axit thap rat it, chi vdi
= . ^ _ ^ - C :
• CipS DCipJ ncJp3
• C^p2
• C i p l
30 K; id Kr 50 Kr Hinh 5. Ty 1$ hat cua qudn thd OM3536 th^
hi^n d ckc c^ phdt pho a tCmg liSu chidu xa 4. Su bi^n ddng ham lupng phytic axit trgn qulin th€ D4 sau diidu xa
Ket qua phan tich HIP cua 100 cSy thupc quan th^ D4 va gidng gdc cho thay, ham lupng phdt pho vo CO trong hat cua quan the tang Idn so vdi gidng gdc.
- O Mu lupng xa lOkr, quan thd chua xuat hi^n cky cd hat bidu hien ham lupng phdt pho vd ca cao (cap 3 trd iSn) hay phytic axit tiiap. Da sd hat th^
hi^n miic phdt pho vd c a thap, hat c^p 1 chidm 96,43% tdng sd h a t
- O cap dd chieu xa 20kr, q u ^ th^ v ^ chua xu^t hien cay cd hat bidu hien ham lupng phytic axit ti-ong hat thap. Tuy nhifen, sd lupng hat bidu hi6n phdt pho vd CO cap 2 (21,88%) trong q u i i thd nay cao hon so vdi q u ^ th^ chidu xa a miic lOkr.
- a h^u lupng xa 30kr, chi 1 ddng D4-60 cd hat bidu hi6n phytic axit th^p, nhimg cung chi vdi 1 hgt va d miic c^p 3.
- O li^u lupng xa 40kr, tuong ti; nhu k^t qua phan tich hat ciia quan th^ 30 kr p h ^ Idn hat th^
3 hat chidm 1,88% tdng sd hat cua qudn th^ (hinh 5;
6).
- 6 li^u X9 30kr, chi mdt cay tfiuOc q u ^ tiid cd hat bi^u hi§n phytic axit th^p, nhung cung chi vdi 2 hgt chidm 1,25%, tdng sd hgt thudc v^ cay cua ddng OM3536-51(hinh6).
- 6 li^u xa 40kr, p h ^ Idn hat ciia ckc ddng thd hi^n lupng phdt pho v6 ca tiong h?t th^p tiic phytic axit tiong hat cao. Q u ^ th^ phan tich chi xult hi^n 1 cay ctia ddng OM3536-70 cd 1 hat bidu hi$n ham lupng phytic axit th^p (miic 3) chidm 0,65% tdng sd hat dupe phan tich (hinh 5).
- d dp dp xii ly 50kr, q u ^ th^ chua xu^t hifin ddng cd hat bi^u hi^n phytic axit th^p.
:)M15.16-.15 OMX19-72
* ' O M 3 5 3 6 - ( i
*- I'.hLian
*- WiliJ rice
• ; « ' « # T
'#«^ •'•''•
Hinh 6. K^t qua phan tich HIP mdt sd ddng thudc q u ^ Ifa^ OM3536 hi6n lupng phdt pho vd co d cap 1 (73,75%), chi cd 1 hat d cjfp 3 thupc ddng D4-61.
- O Mu lupng chieu xa 50kr, quan th^ khdng xuat hi^n ddng cd hat bieu hi^n phytic acid th^p khi phan tich HIP.
5. Sif bi^n dOng h^m lupng phytic axit tr^n qudn thd D8 sau diidu x^
Kdt qua phan tich HIP cua 100 cay cho thay, ham lupng phdt pho trong h^t cua quan th^ bilu hi&n cao nh^t chi ddn c^p 2 so vol 5 c^p cua bang P chuan hay ndi each khac qudn thd khdng cd cay nao cd hat bi^u hi6n phytic axit thap.
Tu kdt qua phan tich cua 5 q u ^ thi tr&n da chiing minh rang, tia gamma cd kha ndng phat sinh cac thd dot bien lam bien ddi ham lupng phytic axit trong hat d cac qudn thd liia sau chidu xa (d M2). Su bidn dpng nay da phan theo chi^u hudng giam ham lupng phytic axit va tang lupng phdt pho vd ca. Hon nua, miic dp bien dpng ham lupng phytic axit trong hat tuy thudc vao dung lupng mSu h?it dupe chidu xa va dac tinh di truyen ciia gidng. D$c bi$t, kdt qua thi
NONG NGHIEP VA PHAT TRIEN NdNG THdN - KY 2 - THANG 5/2012 13
KHOA Hpc C 6 N 0 N C H I
nghiSm cho thSy mSu hat dupe xir ly chilu x? tir 20kr tro 16n, qu5n t h i sau chilu x? b^t diu xuSt h i | n cSy CO hat bilu hi|n c6 hilm luong phytic axit thSp.
Trong 5 quin t h i sau chilu x? chi 4 quin t h i c6 h?t bilu hi$n phytic axit thiip v6i s6 lui;mg hjt rk it
(bang 2), nhilu k i t q u i hrong ti; cung dupe nhilu nhJ khoa hpc h-ong v4 ngoii nude thu dupe trIn nhimg gilng lua kh4c. Nam 2007, Lang KI ctv2007&i sir dung biic xa tia gamma (ngudn Co*°) a lilu lirpng 20kr xii ly ISn hat khd trSn c4c gifing lua OM1490, OMCS2000 da chpn Ipc dupe 4 dbng phytic axit cSp 4 va 32 dong phytic axit cSp 3. Tai HOi nghj ung dung C6ng nghf hat nhSn tji TP. H6 Chi Minh nam 2006,
nhilu b^o c6o ciia cdc n h i khoa hpc nude ngoai cho th^y hp da chpn Ipc dupe nhilu dbng liia dot biin c4 hAm lupng phytic axit trong hat thiip (ciip 4) nhu: t Pakistan trln gilng Basmati, Indonesia tr^n gidng Atomita 4, b ThAi Lan trln gilng Suphanburi 1. D|c bi|t, c4c n h i khoa hpc Trung Q u i c da chpn Ipc va lai tao dupe nhilu gilng liia nli tilng v l phytic axit th^
nhu: OWp*XQZ-l, Os-/p»XS110-l, Os-//»-XSlllM|
Os-lpa-MHS&l. 0s-lpa-Z9B-l,... Trong d6, giong Os- lpa-XS110-l/GO133 da xac dinh gien dilu khiln tinli trang phytic axit thSip flpa-1) djnh vj trln nhijm sic t h i s i 3 ma khdng phSi trln nhilm sac thi s i 2.
STT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25
B i n g 2 . T 6 m tdt k i t q u i cac ddni D6nK phytic axit t h i p
0M81»<;
OM819-21 OM819-31 OM819-35 0 M 8 1 9 4 6 0 M 8 1 9 4 8 OM819-55 O M 8 1 M 5 OM819-72 O M 8 1 M 8 OM490OC OM4900-23 OM490O-24 OM4900-33 OM490046 OM4900-52 O M 4 9 0 M 4 O M 3 5 3 M ; OM3536-29 OM3536-35 OM3536-51 OM3536-72 D 4 G D4-60 D4-62
HIP 1 3 4 3 4 3 3 4 4 3 1 3 3 3 3 3 3 1 3 3 3 3 1 3 3
DP 80 75 75 75 68 69 75 73 78 69 73 70 74 76 78 74 73 64 69 66 63 63 85 78 78
It b i l u h i | n h i m lupng phytic axit t h i p khi phan tich HIP S
8,67 19,33 13,67 14,67 13,00 10,33 7.67 14.67 18.00 11,67 11,50 13,79 10,65 10,33 13,67 9,67 12,37 10,16 14,28 13,93 13,31 12,68 8,47 8,18 13,65
H 136,10
10,93 102.90 97,07 92,20 107.03 100,50 92.87 90,57 90,73 100,10 88,67 94,50 104,07 103,57 90.78 91,17 95,34 89.73 88.88 87.97 82.14 100.19 88,33 87.23
L 27,75 23,17 24.17 22.03 23.87 25.73 26.77 22.53 22,10 22,13 25,22 23.17 26,117 2(1,la 28,70 23,17 24,17 24,08 23,87 23,03 22,93 22,40 27,15 24,03 22,27
F 152,40 105,40 110,60 80,50 100,00 123.30 132,00 84.00 85.31 84.00 12.50 12.00 12.(X) 16,3.-) 24,00 15,011 1()..50 12,76 13,40 12,21 17,00 11,87 13.00 11.60 12.69
E 21,40 13,40 4.80 2,40 8,40 16.40 16.60 9 1 0 5,00 16.00 115.87 96,00 124.63 134.12 116.00 97.00 102.20 101.37 113.00 96.97 93,00 94,30 117,00 134,60 76.37
W 15,52 23,18 24,98 21,05 22.0 22.27 22.27 23 97 24,12 21.52 24.66 24.81 22.14 23.71 20.07 22.24 25,99 23,73 22,84 22,54 24,32 24,13 23,04 23.84 21.39
Y 20,50 42,76 34,35 21.77 26,45 26,08 20,83 26,02 32,69 18,58 32,86 32,86 29,39 32,86 32,85 32.86 32.86 24.43 36,85 30,44 30,16 28,85 22.84 26.24 22,29 Chu thich cac chit & hang mftaia cic cpted pphdn chu thich ctia cac bang phan tich tuong quan.
IV.KErUIMVABtNGHI L K l t l u a n
- Chilu xa Ua y (ngudn C(f°) lln hat lila kho da lam thay dli ham lupng phytic axit trong hat a da s6 cay thulc c^c qudn t h i sau chilu xa. Ham lupng phytic axit frong hat giam d i n miic y nghia (tir c^p 3 tra lln) chi xay ra d 21 cSy thuOc 4 trong 5 quln t h i sau chilu xa vdi cuong &t> til 20kr d i n 50kr. Trong
dd, q u i a t h i OM819 cd 9 cay vdi 4 cSy co hat bieu h i | n p h i t pho vd c c ciip 4. quan t h i OM4900 cd 6 cay d i u t h i h i | n d dip 3, quan t h i OM3536 cd 4 cay va qudn t h i D4 cd 2 cay. Mdi c4y tir c i c quan thi xuS h i | n tir 1 din 3 hilt bilu hijn ham lupng phytic axi' thSp sau khi phan tich HIP d 8 hat.
2. DI nghj
- Tilp tyc h-ong v i chpn Ipc cac t h i hS tilp theo, nhSm chpn dupe c4 t h i dong hpp tu bilu hien him
NONG NGHllP VA PHAT TRIEN NONG T H 6 N - KY 2 - THANG 5/2012
KHOA HQC C 6 N G N 0 H |
lupng phytic axit thatp d^ phuc vu cho cac muc ti^u tao gidng mdi.
TiUUEUTHAMKHAO
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; (1956), Micro-determination of P, Anal. Chem. 28, i pp.1756-1758.
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- Thong tin Khoa hoc Congnghe Hat nhan so 4, Vi§n
• Di truyen Ndng nghi&p.
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58, pp. 21-26.
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Crop Sci AQ, pp. 1601-1605.
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Omon rice 15, pp 29-35
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Academic Press, new York, NY.
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EVALUATE THE FLUCTUATION OF PHYHC ACID CONTENT m SEED FROM MUTANT LINES BY GAMMA RAYS
Nguyen Thi Lang, Pham Thi Be Tu, Pham Van Ut, Bui Chi Buu Summary
Phytic acid is a molecule composed of myo-inositol 1,2,3,4,5,6 hexakis dihydrogen phosphate (Ins P6), a major component in the source of phosphorus (P) reserves of about 50 plants - 8096 of total seed phosphorus (Lott, 1984). At physiological pH in the form of phytic acid have negatively charged ions hold together the complex mineral nutrition creates indigestion. Moreover, phosphorus in the form of phytate or phytic humans and monogastric animals can not absorb, are all discharged polluted environment transitions. In rice OM819, OM4900, OM3536, D4 and D8 are irradiated with gamma rays at 5 degrees lOkr, 20,30,40 and 50kr to create mutant strains with low levels of phytic acid. Results in radiation levels may appear lOkr line grain phytic acid expression is low. At the level 20kr of radiation is three populations OM819, OM4900 and OM3536 with 8 lines for grain phytic acid expression is low. At SOkr extent, appeared seven lines with low nuclear expression of phytic acid 4 populations OM819, OM4900, OM3536 and D4. At tfie level 40kr of radiation thera are 4 populations appear only 5 lines expressed low phytic acid, with 3-line expression levels 3 and 2 lines with level 4 expression. At the level of radiation 50kr only one line appears at level 3 of phytic acid this is OM819 populations. For genome analysis using marker RM 261 with 66.67% of the rice low phytic acid content of the expression analysis of biochemical polymorphisms.
Keyword: Low phytic acid, mutant rice, gamma rays.
Ngudi phan bi^n: PGS. TS. Nguyen Minh Cdng
NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - THANG 5/2012