Cong nghe sinh hgc & Giong cay trong N G H I E N C l T U N H A N G I O N G C A Y T U C D O A N {Dipsacus japonicus)
B A N G P H i r O N G P H A P N U O I C A Y M O
Khu3t Thi Hai Ninh', Phan Thi Trang', Nguyin Thi Thcr', Biii Van Th5ng', Vu Van Hilu^
'Truang Dai hoc Lam nghiep
Trung tdm Nghien ciru giong cdy trong Dgo Due - Hd Giang TOM TAT
Nhan giong Tuc doan (Dipsacus japonicus Miq) bang phuong phap nuoi cdy m6 da duac nghien ciru thanh cong, ket qua cho thSy: Khii trung heit bang HgCl2 0,1% trong thoi gian 1 phiit va Javel 5% trong thoi gian 20 phut cho ket qua 60% hat sach nay mSm. Khii tning ch6i bang HgClj 0,1% trong thoi gian 2,5 phiit, dat ket qua 22,2% mlu sach nay choi. Moi truong dinh dirong co ban MS b6 simg 0,4 mg/l BAP; 0,4 mg/l TDZ thich hgp de nhan nhanh choi, voi 100% mlu tao cum choi, 5,2 choi/cum, chieu cao choi dat 3,4 cm Tang tnrong choi fren moi truang dinh duong co ban MS bo sung 0,4 mg/l BAP; 0,2 mg/lKinetin; 0,1 mg/l NAA, chieu cao choi dat 7,5 cm. Moi truang dinh duang ca ban MS bo sung 0,4 mg/l NAA phii hgp de tao cay con hoan chinh,vdi ti le choi ra re dat 100%, chat lugng re tot. Cay mo trong tren gia the phoi trgn 50% dat tang B vai 25% cat va 25% trau hun, cho ty le cay song la 60% sau 4 tuan ra ngoi.
Tir khoa: Cum choi, nhan giong, nuoi cay mo, Tuc doan.
1. DAT VAN DE
Cay Tuc doan cdn gpi Sam nam, Dau vii, R I ke, Meo xieng khoang, co ten khoa hpc Dipsacus japonicus, thupc hp Tuc doan (Dipsacaceae). Tuc la noi, doan la diit vi ngudi xua cho rang vi thudc cd tac dung ndi dupe gan xuofng da diit. Theo ddng y, Tuc doan cd vi dang cay, tinh hai dn, co tac dung bd can ich than, ndi lien gan cdt, thdng huyet mach, cam mau, giam dau. Chii tri can than hu, lung dau, chan yeu, gay xuong, bong gan, dpa say thai, an thai, chi huyet, chiia bang lau ddi ha (Dd Tat Lpi, 2015). Ngoai ra, tinh dau Tuc doan cd tac dung chdng lai con trung gay hai nhu Mpt bdt dd (Tribolium castaneum) thudng tan cong cac san pham ngu cdc va Mpt hai ngd (Sitophilus zeamais) (Zhi Long L\\xet et ai, 2013). Tuc doan la mdt trong cac loai cay dupe lieu dupe qui hoach trdng d cac viing niii cao va vung niii trung binh cd khi hau A nhiet ddi (Theo Quyat dinh so 1976/QD-TTg ngay 30/10/2013 ciia Thu hidng chinh phii va Quy^t dinh sd 179/QD-BYT ngay 20/1/2015 ciia Bp Y tg). Vi vay, viec nhan gidng va gay trdng Tuc doantai nhiing khu phan bd ciia chung la het sue can thiet.
Hien nay, nhu can trong va ngoai nude ve Tuc doan rat Idn, viec khai thac nhiing cay mpc hoang khdng dap irng dupe ngudn cung.
Mac dll cd the nhan gidng cay Tuc doan bang phuong phap sinh dudng hoac hihi tinh nhung cay me de tihanh kem, hat thudng duac thu hoach vao mua ddng gia ret (thang 11, 12) d vimg niii cao do do gap nhieu kho khan (Dd Huy Bich, 2006; Xu hand Wang Wei, 2002).
Mat khac, do bd phan dung lam thudc ciia cay Tuc doan la re, nen khi khai thac ngoai ty nhien ngudi dan dao ca cay do vay cay khdng cdn ca hpi tai sinh. Vi vay, nhan gidng cay Tuc doan bang phuang phap nudi cay md se giiip tao ra so lugng Idn cay con trong thdi gian ngan, cd the dap ung ngudn gidng cho cac viing san xuat dupe lieu, nham nang cao thu nhap cho ngudi dan va phuc vu cdng tac bao tdn ngudn gen cay thudc.
2. PHirONG PHAP NGHIEN CUtJ 2.1. v a t ligu
Vat lieu: Chdi va hat Tuc doan do Trung tam Nghien cuu Gidng cay trdng Dao Due - Ha Giang cung cap.
2.2. Phwonng phap nghien ciiu
Nghien ciiu dupe tien hanh theo cac bude khir tning mau, nhan choi, tao cay con hoan chinh, va trong cay con ngoai vudn uom. Mdi cdng thiic thi nghiem dupe bd tri 3 lan lap, mdi lap 30 mau.
2.2.1. Phuffng phdp bo tri thi nghiem Tao mdu sach vd nuoi cdy khoi dgng:
TAP CHI KHOA HOC VA CONG NGHE LAM NGHIEP SO 2 - 2019 11
Cong nghe sinh hgc & Gidng cdy trong
1) Tgo mdu sach tie hat
Hat dupe lira chpn trudc khi khir tning (gdm cac hat chac, may, nhirng hat ndi tren mat nude deu bi loai bd). Sau dd, hat dupe rira sach bang nude, ngam va lac deu trong nude xa phdng loang 2 - 3 phiit, trang lai nhieu lan bang nude sach.
Man tiep tuc dupe nia bang nude cat vd tning 2 - 3 ISn (mdi lan I5c 2 - 3 phiit), sau dd khu hung bang HgClz 0,1 % va Javel 5% vdi thdi gian khac nhau. Sau moi lan khu trimg bang hda chat, man can dupe trang rira bang nude cat vo triing 2 - 3 lan, mdi lan tu 2 - 3 phiit.
2) Tgo mdu sack tic choi
Mau nudi cay dupe chpn la cae cay Tue doan 1 nam tudi, than map la phat trien tdt, khdng sau benh. Sau do cat het la, chi de lai cudng la dai khoang 0,5 cm va cat bo re, rira mau dudi voi nude chay, roi diing chdi long rira mlu trong nude xa phong loang, rua lai
bang nude sach.
Mau tiep tuc dugc dua vao box cay rira bing nude cat vd tning 2 - 3 lan (mdi lin ISc 2 - 3 phut), sau dd khu tning bang HgCl2 0,1%
trong thdi gian 2,5; 3; 3,5 va 4 phiit. Sau khi xu ly mau, can trang bang nude cat vd tning 2 - 3 lan, mdi lan tir 2 - 3 phut.
3) Nuoi cdy khai dong
Sau khi khir tning, cac mdu hat va ch6i dugc cay trenmdi trudng dinh duong ca ban MS bd sung 30 g/l sucrose; 6 g/1 agar. Mau cky dupe nudi trong tdi 1 - 2 tuan, khi cac mlu hk dau nay mam va tai sinh chdi thi chuyen sang nudi sang. Trong qua trinh nudi mau, thudng xuyen kiem tra loai bd ngay nhiing mau nhiem ra khdi phdng nudi, tranh lay nhiem cho cac mau khac-
Chi tieu theo doi: Sd mau sach, sd mau sach nay chdi, sd mau sach bi chet sau 4 tuan vao man.
Hinh 1. H^t Tuc doan (hinh trai) va cay Nhdn nhanh choi:
MSu sach dugc tao ra d thi nghiem fren (gom chdi va cay mam) dugc cky chuygn sang moi trudng dinh duong MS cd b6 sung cae loai chat di^u hda sinh trudng (BAP, Kinetin, NAA va TDZ) d cac ndng dp khac nhau, 30g/l sucrose, 6 g/l agar.
Chi tieu theo doi; Sd mlu tao cum chdi, sd chdi/eum va chieu cao chdi sau 3 tuSn nuoi cky.
Kich thich ra re t^o cdy hodn chinh:
Nhiing chdi khde manh, xanh, map cd chigu dai tir 5 - 7 cm se dugc eay chuySn sang moi trudng tao YI la mdi trudng khoang co ban MS bd sung 30 g/l sucrose, 6 g/l agar, IBA hoac NAA CO ndng dp 0,2 - 0,5 mg/l.
(hinh phai) dirffc lya chon de vao mau Chi tieu theo doi: Sd chdi ra rl, sd rl/cay va chiSu dai r i sau 3 tuin nudi cay.
Anh hiromg cua loai gia the den kha nang song va sinh trudng cua cay in vitro tai vudn uom.
Cae binh cay md dugc hu4n luyen dudi sanh sang tan xa h-ong thdi gian 1 tuSn (cay co chieu cao > 4 cm, cd la va bd r l phat triSn, cay cung cap). Diing panh g i p cac cay trong binh ra ngoai, sau dd rira sach bd re bSng nuoc sach de loai bd agar, d l cay rao nude va cky tren cac loai gia thS khac nhau: phdi trpn giira dat doi tang B, eat vang va triu hun d l nghien ciiu kha nang sdng va sinh trudng trong giai doan vudn uam.
12 TAP CHI KHOA HOC VA CONG NGHE LAM N G H I E P SO 2 - 2019
Cong nghe sinh hgc & Giong cdy trdng Chi tieu theo doi: Ty le cay sdng, chieu eao
cay va ehat lugng cay sau 4 tuan ra ngdi.
2.2.2. Phuffng phdp xif ly s6 lieu
So sanh giua cac cdng thiic thi nghiem ve ti le mau sach, ti le mau tao cum chdi, ti le choi ra r l bang tieu chuan khi binh phuang (x^). So sanh ket qua cua cac cdng thirc thi nghiem ve sd lugng chdi/cum, chieu eao chdi, chieu dai re va sd lugng re/cay bang phan tich phuong sai mdt nhan td.
Sd lieu da thu thap dupe xir ly bang phan mem SPSS (Nguyen Hai Tu^t va Nguyen Trpng Binh, 2005) va phan mem Excel.
2.3. Dia diem nghien ciru
Thi nghiem dugc tien hanh tai phdng nudi Bang I. Anh hir&ng cua thoi gian khu* trung
c4y md - t l bao thuc vat cua Vien Cdng nghe Sinh hpc Lam nghiep, Trudng Dai hpc Lam nghiep.
Cac mdi trudng nuoi cay dupe dieu chinh pH =- 5,8, chieu sang lOh/ngay, vdi cudng dp anh sang 2000 - 3000 lux, nhiet dp phdng nudi 25 ± 2"C
3. K E T QUA VA T H A O L U ^ N 3.1. Tao mau sach
3.1.1. Tgo mdu sack tir hat
Tao mlu sach Tuc doan hi hat bang viec sir dung hda chit la HgCl2 0,1% va Javel 5% vdi thdi gian khac nhau de khir trimg. Ket qua thu dugc sau 4 tuan nudi cay dugc the hien d bang 1.
den kha nang tao mau sach va nay mam tir h^t Thoi gian
xu Iy (phut) HgCl2 Javel
0,1% 5%
Mau sach Mau sach nay mam
Mau chet
1 2 3 4 5 6 7 8 9
1 2 3 1 1 1 2 2 2 Sig
-
15 20 25 15 20 25
10 22 34 42 62 72 66 72 79
11,1 24,4 37,8 46,7 68,9 80,0 73,3 80,0 87,8
9 18 13 28 54 25 24 27 21
10,0 20,0 14,4 31,1 60,0 27,8 26,7 30,0 23,3 0,0001
1 4 21 14 8 47 42 45 58
1,1 4,4 23,3 15,6 8,9 52,2 46,7 50,0 64,4
So lieu bang 1 cho thay khi su dung HgCb 0,1% va Javel 5% de khii tning mau hat vdi thdi gian khac nhau da cd anh hudng ro ret den ti le m l u sach nay mam (sig < 0,05). Khi khu' trung hat chi bang HgCl2 0 , 1 % trong thdi gian 1 - 3 phiit cho ti le mau sach nay mam thap (5,6 - 8,9%). K i t hpp HgCl2 0 , 1 % va Javel 5%
ti le mau sach nay mam tang len dang ke (23,3 - 60%). Trong do, xii ly hat bing HgCli 0 , 1 % trong thdi gian 2 phiit ket hpp Javel 5% tu 15 - 25 phut cho ti le mau sach kha eao (73,3 87,8%), hiy nhien m l u sach may mam chi dat tir 23,3 - 30%. Giam thdi gian xii ly mlu bing
HgCl2 0,1% xudng 1 phiit ket hop Javel 5% tir 1 5 - 2 5 phiit cho ti le mau sach nay mam kha cao (27,8 - 60%). Nhu vay, khi thdi gian khu triing hat bang HgCl2 0,1 % qua lau se gay ddc va lam chet hat, do dd de giam miic dp nhiem ddc cua hat can xu ly HgCU 0,1% trong vdng 1 phiit ket hop javel 5% trong thdi gian 20 phut cho hieu qua tdt nhat (60% hat sach nay mam) (hinh 2a).
3.1.2. Tgo mdu sach tie choi
Ket qua tao mau sach tir chdi khi xOr ly bang HgCb 0,1% vdi thdi gian khac nhau dugc thi hien d bang 2.
TAP CHI KHOA H O C VA CONG NGHE L A M N G H I E P SO 2 - 2019 13
Cong nghe sinh hoc & Gidns cay trong
Bang2. Anh hirffng cua IhM gian khii triing dSn kha nang tao mSu sach va lai sinh choi STT Thftlgiaaiirly |v[3„ sach Mau sach Mlu sach chet
HgClj 0,1% tai sinh choi
1 2 3 4
(phut) 2,0 2,5 3,0 3,5 Sig
N 8 30 21 28
%
8,9 33,3 23,3 31,1
N 8 20 16 7
8,9
%
22,2 17,8 7,8 0,013
N 0 10
5 21
0,0
%
11,1 5,6 23,3
Sd lieu d bang 2 cho thay khi sir dung HgCl2 0,1% d l khir triing m l u vdi thdi gian khir nhau da cd anh hudng ro ret din kha nang tao mlu sach (sig < 0,05). Khi tang thdi gian khu trimg m l u bing HgCl2 0,1% tir 2 - 3,5 phiit ti le mlu sach ciing tang len (8,9 31,1%), tuy nhien ti le mlu sach nay choi lai khdng ti le thuan. Khir trimg 2 phiit cho ti le mau sach tai sinh choi thap nhat (8,9%) va khong xuit hien mau sach bi chet. Khi tang thdi gian khir tning mlu len 2,5 - 3 phiit ti le man sach nay choi tang len dang ke (17,8 - 22,2%). Nhung khi tang thdi gian khu hiing len 3,5 phut mlu sach tai sinh chdi giam di dang k l (7,8%), ti le mau sach chet kha cao
(23,3%). D I dang nhan thay kha nang chju thuy ngan ciia m l u choi Tue doan rat kem. Vi vay, n l u tao m l u sach tir chdi nen xii ly bing HgCl2 0,1% trong thdi gian 2,5 phut la thich hgp (vdl ti le m l u sach nay chdi dat 22,2%) (hinh 2b).
3.2. Anh hu-dng cua chat flieu hoa sinh tru'dng d i n k h a nang tao cum ch6i
Nhirng m l u song khoe manh, khong nhilm nim va khuin dugc cay chuyen sang moi trudng dinh dudng co ban MS bd sung cac chit diiu hoa sinh trudng BAP, Kinetin, NAA va TDZ d cac ning dp khac nhau d l nghien cint kha nang tao cum chdi. Ket qua thu dupe sau 3 tuan cly chuyin duoc the hien d bang 3.
STT
1 2 3
4 5 6
7 8 9
B a n g 3
B A P 0,3 0,4 0,5
0,3 0,4 0,5
0,4 0,4 0,4
. A n h hirdng ; cHa chSt aieu hoa sinh tririJTtig Sen k h a l a o cum Chat D H S T (mg/l)
Kinetin
-
Trung binli 0,2 0,2 0,2 Trung binh
- -
Trung binh Sig
N A A
0,1
0,1 0,1 0,1
-
T D Z
-
-
0,3 0,4 0,5
MSu lao cum Choi N 28 4 3 34 35,0
45 55 47 49,0
90 90 90 90 1
%
31,1 47,8 37,8 38,89 50,0 61,1 52,2 54,44 100 100 100 100 3,0001
So tirong choi/cum
1,8 2,2 1,6 1,87 2,7 3,2 2,5 2,80 4,1 5,2 4,5 4,60 0,0001
Choi Chieu cao
chdi (cm) 6,8 7,1 6,4 6,77 7,6 7,5 6,9 7,33 3,5 3,4 3,2 3,37 0,0001
So lieu d bang 3 cho thay, khi ket hgp cac chat dieu hoa sinh trudng vdi nong dp khac nhau cd anh hudng ro ret den ti le mau tao
cum choi, sd chdi/cum va chieu cao choi.
Trong mdi trudng nuoi cly chi bd sung BAP (0,3 - 0,5 mg/l) va 0,2 mg/l NAA ti le mlu tao 14 TAP CHI KHOA H O C VA C 6 N G N G H E L A M N G H I E P SO 2 - 2019
Cong nghe sinh hgc & Giong cdy trong cum chdi trung bmh dat 38,89%, s6 choi/cum
hung binh dat 1,87. Khi kit hgp BAP (0,3 - 0,5 mg/l), 0,2 mg/l NAA va 0,2 mg/l Kinetin ti le mau tao cum chdi tang len dang ke (trung binh 54,44%), sd choi/cum cung tang (trung bmh dat 2,80 chdi), tuy nhien van d miic thap.
Mac du vay, su ket hgp giiia BAP, Kinetin va NAA deu cho chdi phat trien kha tdt ve chieu eao (tir 6,4 - 7,5 cm). Mdi trudng cd su ket hgp giua TDZ (0,3 - 0,5 mg/l) va 0,4 mg/l BAP deu cho 100% chdi tao cum chdi, sd ehdi/eum cung tang len (trung binh dat 4,6 chdi/cum), nhung chieu cao choi ban che
(tnmg binh dat 3,4 cm). Nhu vay, cd the lira chpn mdi trudng MS bd sung 0,4 mg/IBAP;
0,4 mg/l TDZ cho nhan nhanh chdi va mdi hrudng MS bd sung 0,4 mg/lBAP; 0,2 mg/l Kinetin; 0,1 mg/l NAA dl nang cao chit lugng chdi (hinh 2c).
3.3. Anh hirdng cua chSt di§u hoa sinh trudng din kha nang ra re
Nghien cim kha nang ra re in vitro Tuc doan bing viec su dung mdi trudng dinh dudng ca ban MS bl sung rieng re NAA, IBA va ddi chiing (khdng bo sung IBA va NAA). Kit qua tao rl in vitro dugc the hien d bang 4.
Bang 4. Anh hirdng ciia nong do chat dieu hoa sinh truong den kha nang ra re
CTTN NI N2 N3 N4
11 12 13 14
ChaiDHST (mg/l) NAA
0,2 0,3 0,4 0,5 Trung binh
Sig
-
Trung binh DC Sig
IBA
-
0,2 0,3 0,4 0,5
Ti lg ra rg
100 100 100 100 100 100 100 100 100 100 83,3
S6 lu'OTig re (c^i)
2,8 3,2 4,2 3,5 3,4 0,0001
1,4 1,6 1,8 2,2 1,8 1,3 0,068
Chigu dai rg (cm)
1,8 2,5 2,8 2,2 2,3 0,0001
1,3 1,7 1,5 1,3 1,5 1,2 0,003
Thod gian ra rg (ngay) 14 13 10 16 13,3 13 12 15 17 14,3 19
Chat liroTig re
TB Tot Tot TB
TB TB TB TB xau Ghi chd: Re tot: map vd trdng, Re trung binh. map vd hai vdng; Re xdu re mdnh vd den
Sd lieu d bang 4 cho thay sir dung chat dieu hda sinh trudng trong moi hirdng nudi cay cho ket qua tdt hon so vdi cdng thiic ddi chirng (khdng cd chat dieu hda sinh trudng). Dieu nay thi hien rd d thdi gian ra re ciing sdm (hi 10 din 17 ngay), ti le choi ra rl rit tdt (100%) khdng con thay xuit hien re xau chi ed loai re cd chit lugng tir trung binh trd len, trong khi do cdng thiic ddi chiing thdi gian ra re 19 ngay, ti le chdi ra re chi dat 83,3%.
Khi sii dung NAA, IBA d cac nong dp khac nhau bd sung vao mdi trudng tao cay hoan chinh deu cho ket qua tdt. Trong dd, tat ca cac
cdng thiic mdi trudng nudi cay deu cho ty le ra re dat 100% (khong cd sy sai khac), so lugng re, chieu dai re, chat lupng bd re va thdi gian ra re da cd su khac nhau. Bd sung NAA vao mdi trudng nudi cay da tao ra 3,4 re/cay, chieu dai re trung binh 2,3 cm, thdi gian ra re trung binh 13,3 ngay. Bd sung IBA vao moi trudng tao cay hoan chinh da tao dugc 1,3 re/cay, chieu dai re trung binh 1,2 em, thdi gian ra re trung binh 13,3 ngay.
Nhu vay, cd the nhan thay bd sung 0,4 mg/l NAA vao mdi trudng nuoi cay tao re la phii hgp nhat trong nghien ciiu tren (hinh 2d).
TAP CHI KHOA HOC VA CONG NGHE LAM NGHIEP SO 2 - 2019
Cong nghe sinh hgc & Giong cdy trong 3.5. Anh hvdng ciia loai gia t h i flgn kha nang sdng va sinh tnrdng cua cay in vitro tai virdn uom
0 giai doan dau khi dua cay ra trong, thanh phin gia thi trong mdt bau cd vai trd quan
trpng giiip cay cd kha nang hut nude va chSt dinh dudng tdt, ddng thdi khdng lam thii rl cay la vd cung can thiet. Thanh phan gia thi rugt bau va tinh hinh sinh trudng cua cay con sau 4 tuIn dugc the hien d bang 5.
Bang 5. Anh hutfng cua cac loal gia the den kha nang song va sinh tru-oTig ciia cay in viuo
STT
1 2 3 4
Cong thuc
GTl GT2 GT3 GT4
Loai gia the
^ " Cat tang
R vang
100 0 50 25 75 0 75 25
Sig
sif .
0 25 25 0
Tile cly song
(%)
10 60 16 23 0,0001
Thu thap s6 lieu sau 4 tuan Chigu
cao cSy (cm) 4,1 6,3 5,1 4,9 0,0001
Chat lirtfng cay con
Cay cao 3,6 - 4,2 cm, nho, la mong xanh non
Tot, cay cao 5,8 - 6,5 cm, map;
la day, xanh non
Tot, cay cao 4,5 - 5,6 cm, kha map, la xanh non
Tot, cay cao 4,5 - 5,4 cm, kha map, la xanh non
Sd lieu d bang 5 cho thay, loai gia the khac nhau CO anh hudng rd ret den ti le cay song va sinh trudng chieu cao cay. Trdng cay tren gia the la 100% dat tang B ti le sdng thap nhat (10%), cay sinh trudng phat trien kem nhat
(cay cao trung binh 3,6 - 4,2 cm). Tren gia the the trpn 50% dat tang B; 25% cat vang va 25%
trau hun ti le cay sdng cao nhat (dat 60%), sinh trudng va phat trien tdt nhat (cay trung binh cao 5 8 - 6 5 cm) (hinh 2e).
*Vv 'V, i, v.-¥"-1R'
Hinh 2. Mot so hinh anh cay Tuc doan trong cac giai doan nhan gilng in vitro a: Hat nay mam, b: Mlu sach nay chli; c: Cum choi, d: Cay hoan chinh; e: Cay con trong ngoai vu'dn uom.
TAP C H i KHOA HOC VA CONG NGHE LAM N G H I E P SO 2 - 2019
Cong nghe sinh hgc & Gidng cdy trong 4. KET LUAN
Khir trimg tao mau sach Tuc doan tir hat khi sir dung dung dich HgCl2 0 , 1 % trong vdng 1 phiit va Javel 5% trong thdi gian 20 phiit dat 60% hat sach nay mam. Khir tning choi bang dung dich HgCl2 0 , 1 % ttong thdi gian 2,5 phiit cho ti le mau sach tai sinh choi dat 22,2%.
Nhan nhanh chdi tren mdi trudng dinh dudng c a ban MS bd sung 0,4 mg/l BAP; 0,4 mg/l TDZ, ket qua dat 100% m l u tao cum chdi, 5,2 chdi/cum, chieu cao chdi dat 3,4 cm.
Tang trudng chdi tren mdi trudng khoang eo ban MS bd sung 0,4 mg/l BAP; 0,2 mg/l Khietin;
0,1 mg/l NAA, chieu cao chdi dat 7,5 cm.
Ra re tao cay hoan chinh tren moi trudng dinh dudng co ban MS bd sung 0,4 mg/l NAA, ket qua dat 100% chdi ra re, thdi gian ra re sau 10 ngay, chat lugng re tdt.
Cay md trdng tren gia the 50% dat tang phdi h:dn vdi 2 5 % cat va 2 5 % triu hun, cho ty le cay sdng la 60% sau 4 hian ra ngdi.
TAI LIEU THAM KHAO
1. D6 Huy Bich, Dang Quang Chung, Bin Xuan Chuong, Nguyen Thuang Dong, Do Trung Dam, Pham Van Hiin, Vu Ngoc Lo, Pham Huy Mai, Pham Kim Man, Doan Thi Nhu, Nguyin Tap, Trin Toan (2006).
Cdy Thudc vd dgng vdt lam thuoc Viet Nam. NXB Khoa hoc va KI thuat, Ha Noi.
2. D6 Tdt Lai (2015). Nhihtg cdy thuoc vd vi thuoc Viet Nam. NXB Thai dai.
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Khai thdc vd sic dung SPSS de xit ly so lieu trong lam nghiep. NXB Nong nghiep.
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R E S E A R C H O N P R O P A G A T I O N 0¥ Dipsacus japonicus B Y T I S S U E C U L T U R E
Khuat Thi Hai N i n h \ Phan Thi Trang\ Nguyen Thi Tho', Bui Van Thang\ Vu Van Hieu^
Vietnam National University of Forestry Research Center for plant breeding Dao Due - Ha Giang S U M M A R Y
Research on the propagation oi Dipsacus japonicus by tissue culmre, belonging to Vietnam National University of Forestry, showed that in HgCl2 0 . 1 % for 1 minute and have 5% for 20 minutes the portion of clean sprouted seeds is 70 - 83.3%, HgCl2 0 1% for 2 minutes the portion of clean sprouted expands is 22.2%. In the shoot regeneration most appropriate medium is Murashige and Skoog (MS) medium supplemented with 6- Benzylaminopurine (BAP) 0.4 mg/l; Thidiazuron (TDZ) 0.4 mg/l (the portion of shoot regeneration expands is 100% with 5.2 shots per explants, length of the shoot is 3.4 centimeter). Then improve shoot quality is MS medium supplemented with BAP 0.4 mg/l, Kinetm 0.2 mg/l, a-Napbtbaleneacetic acid (NAA) 0,1 mg/l (with shoot height of 7.5 cm). The best medium for shoot rooting is MS with supplement 0.4 mg/l NAA (roots have appeared after 10 days, rate of rooted shoots is 100%, and roots having good quality). The plantlets are planted in mixes 50% of the soil with 25% sand and 2 5 % of the husk at the nursery (with a survival rate of 60% after four weeks).
Keywords: Dipsacus japonicus, in vitro, multi-shoot, propagation.
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TAP CHI KHOA HOC VA CONG NGHE LAM NGHIEP SO 2 - 2019