; - ' Khoa hcK Cong nghiN6ng rj"
Study on growth and development ability of new maize varieties in Thanh Hoa province
Kieu Quang Luan, Kieu Xuan Dam, Nguvi-n Xuan Sinh, Hoang Thi Thanh Hoa, Nguven Thanh Tflan Abstract
The gro\sth and development abihty of some new hybrid maize varieties was evaluated during Autumn Winter of 2018 and Spring of 2019 in Thanh Hoa pro\ince. The experiments were arranged in completely randomized block design (CRBD) with 4 repetitions. The planting density was 57.000 plants/ha; the fertilizer appheation per ha uas 2,500 kg of organic mineral fertilizer -i- 450 kg Urea + 700 kg Superphosphate + 200 kg Kalielorua. The results showed that the growth duration of hybrid maize varieties was 97 - 99 days in Autumn Winter of 2018 and 115 - 119 days inSpring of 2019 in Yen Dinh district, Thanh Hoa province; these hybrid maize varieties belonged to medium growth duration and was suitable to ecological condition and cultivation custom of the local people. The variety TM 18-3 had high harvesting yield of 75.41 quintals/ha in Autumn Winter of 2018. The variety TM18-3 had high harvesting yield of 74.05 quintals/ha. and the variety VS201 had high har\'esting )ield of 74.53 quintals/ha in Spring of 2019; these two varieties had high harvesting yield, stability, adaptabihty in Thanh Hoa province.
Keywords: hybrid maize variet\'. yield, stability, gro%vth and development
Ngay nhan bdi: 10/4/2020 NgUdi phan bi^n: TS. Pham Xudn Liem Ngdy phan bien: 17/4/2020 Ngay duyet dang: 29/4/2020
DANH GIA DA DANG DI TRUYEN CUA 24 D 6 N G N G O D O N BQI K £ P
TAO RA BANG PHU'ONG PHAP KICH TAO DON BOI
Nguyen Dflc Thdnh', Dang Ngpc Hg', Nguyen Van TrUdng'. Nguyen The Hiing- TOM TAT
Trong nhdng nam gan day, cdng nghe kich tao ddn bdi trong chpn lao gidng ngd lai dang du^c nhieu nUdc tren the gidi nghien cdu vd flng dung rpng rai. Vdi sfl giiip dd eua CIMMYT, Vi?n Nghien cflu Ngd da tiep nhdn thdnh cdng cdng nghe tao ddng ddn bdi kep va tao ra nhieu dong ddn bdi kep. Ddnh gia da dang di truyen. phan nhdm Uu the lai 24 ddng ddn bpi kep (DH) bdng chi thj phan tfl SSR dvu Xuan 2017. Qua danh gia cho cho thay da sd cae ddng ed dp thuan di truyen cao, ty le dong hop td ldn hdn 90%. Vdi h? so tuong ddng di truyen 0,30 cdc ddng ngd chia lam 2 nhdm Idn {21 ddng va 3 ddng). d he sd tUdng ddng di truyen 0,32 nhdm Idn I dUde chia thdnh 2 nhdm thfl cap (15 ddng vd 6 ddng).
Td khda: Cdng nghe kich tao ddn bdi. da dang di truyen, ddng ngd ddn bdi kep
I. DAT VAN DE thuan thdng qua ede phUdng phdp lai dinh, lai luan ChUdng trinh chon tad gidng ngd lai flng dung g'^d Id vd eflng quan trpng. Tuy nhien. viec ddnh gia cdng nghe sfl dung eay kich tao ddn bpi tai Viet Nam cdc dac tinh hinh thai ngodi rudng eac con Iai ton rat trdng nhflng nam qua da dat dfldc nhieu thdnh cdng. nhieu thdi gian vd cdng sflc. Do dd, can dng dung chi Thdng qua 2 dd dn: "Dd dn san xuat gidng ngd lai thi phdn tfl DNA giup eho viec lai lao gidng cd dinh giai doan 2011 - 2015", "Dfl an nghien edu flng dung hudng va nhanh chdng hdn. Chi thi phan tfl DNA cdng nghe kich tao ddn bdi trong chpn tad gidng cd nhieu flu diem nhU do Ifldng true tiep vat lieu di ngd Iai" va chUdng trinh hpp tdc vdi CIMMYT, Vien truyen, sd lUpng dau trong quan the ldn, khdng chiu Nghien cdu Ngd dd thdc hien thdnh cdng "Cdng anh hUdng mdi trUdng. nhanh chdng va chinh xac nghe tao ddng ngd ddn bdi kep bdng phUdng phap (Nguyen Thi Lang vd ctv., 2005). Chinh nhd nhdng sfl dung cay kich tao ddn bdi" tai Met Xam Uu diem ndy, eac chi thj phdn tfl nhu Restriction De tao ra dUdc mdt td hdp lai eo flu the lai tdt Fragment Length Polymorphism (RFLP), Random viee ddnh gia dfldc khd ndng ket hdp cua cac ddng Amplified Polymorphism DNA (RAPD), Amplified ' \ien Nghien cflu Ngd; - Hpc \'ien Ndng nghiep Viet Nai
•iKhcj "z: Cong ngt^e '.'O-j "gh'ep Viet Nam-S6A{ II3''2020 Fragmcnl Length p o h i n o r p h i s m (AFLP), Simple
Sequence Repeat (SSR)... da dUcJc ling d u n g n h i e u trong c h p n g i o n g p h a n tii. T r o n g d o , chi thi SSR to ra hieu q u a va da d i l p c sii d u n g t r o n g n h i e u c o n g trinh n g h i e n ciiu k h o a h o c t r e n the gicti va t r o n g niioc trong viec xac d i n h giong. kieu di t r u y e n , sU phat sinh loai. da d a n g di t r u y e n , t i n h k h o a n g each di truyen d e tien d o a n itu the lai...
II. VAT l l 6 U VA PHUONG P H A P NGHIEN CtfU 2.1. Vat li^u n g h i e n cijiu
- \'.it lieu t r o n g thi n g h i e m d a n h gia d o n g g o m 24 dong ngo d o n boi k e p v^ 2 d o n g doi chtfng (Bang 1).
Bang 1. Ten v i nguon goc d6ng Nguon goc
22 chi thi SSR t r o n g n g h i e n ciia (John L PortM'ood el al, 2018) ( B a n g 2).
Bang 2. Danh sich moi sii dung
Ten d o n g
DI D2 D3 D4 D.-;
D6 D7 D8 D9 DIO D l l D12 D13 D14 D15 D16 D17 D18 D19 D20 D21 22 D22 23 D23 21 D24 D6 doi chtjfng 1 (DC 1) 1IL6 ddi chflng 2 , (DC 2)
20 21
Gidng ngd lai d d n NK67 Gidng ngd lai ddn NK4300. Syngenta Gidng ngd lai kep NK4300 x NK67 • Gidng ngd lai k^p NK4300 x NK67 Gidng ngd lai ddn NK7328 Cndng ngd lai don NK7328 Gidng ngd lai d d n DK8868
Gidng ngo lai ddn DK8S68 , Gidng ngd lai ddn DK8S68
Gidng ngd lai ddn DK9901 Gidng ngd lai d d n DK990I
Gidng ngo lai ddn DK9901 1 Gidng ngo lai ddn DK9901 l Gidng ngd lai ddn CP333
Gidng ngd lai d d n CP333 Gidng ngd lai d d n CP333 Gidng ngd lai d d n CP333 Gidng ngd lai d d n 30Y87 Gidng ngd lai ddn 30Y87 Gidng ngd lai ddn VN8960 Gidng ngd lai d d n 30Y87 Gidng ngd lai d d n 30Y87 Gidng ngd lai ddn LCH9 Giong ngd lai don LCH9
\'ien Nghien cflu Ngd
\'ien Nghien edu Ngd
STT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23
Ten moi u m c l 3 9 9 phi374118 phii 12 phil00175 phi452693 phi233376 phi423796 phi78 phi84 u m c l l 6 1 phi 127 u m c l l 9 6 phi93 m n c l l 0 9 ujncl304 uracl279 u m c l 136 phil01049 phi44S880 u m c l 153 n c l 3 3 umc2359 u m c l 862
\1 tri t r e n NST 3,07 3,02 7,01 8,06 6,06 8,03 6,01 6,05 10,04 8,06 2,08 10,07 4,08 4,10 8,02 9,00 3,10 2,09 9,45 5,09 2,05 9,07 1,11
Kieu lap (CTAG)5 ACC AG AAGC AGCC CCG AGATG AAAG GAA GCTGGG AGAC CACACG AGCT AG (TCGA)4 (CCT)6 GCA AGAT AAG (TCA)4 GTGTC (AAG)4 GA
Kich thu^c
(bp) H I . 127 217 - 238 1 3 6 - 160 1 1 7 - 141 125 142 121 122 150 134 112
145 154 141 166 156 158 126 1 3 7 - 161 274 - 294 1 0 4 - 116 1 2 9 - 137 9 2 - 101 132- 159 230 - 274 1 7 2 - 188 1 0 5 - 114 1 1 0 - 115 1 1 8 - 150 121 150 Nguon: Bo mdn Cong nghe sinh hgc, Vien Nghien cUu Ngo
2.2. P h U d n g p h d p n g h i e n c d u
Thi n g h i e m flng d u n g ehi ihi p h d n td SSR p h a n tich da d a n g di t r u y e n va p h a n n h d m flu the lai 24 d d n g ngd d d n bdi kep: Tdeh D N A l o n g sd t h e o p h d d n g p h a p efla S a g h a i - M a r o o f va c d n g ldc vien (1984). D i e n di san p h a m PCR: t h e o quy t r i n h c u a A M B I O N E T (2004). Liet ke tat ca cac b a n g xuat hien d tat ca gieng. T r e n m d t gieng, b a n g ed xuat hien c h o d i e m 1, k h d n g xuat hien cho d i e m 0 va n h f l n g d d n g khuyet sd lieu hoac b a n g k h d n g chdc chdn cho diem 9.
T h d n g q u a ede dfl lieu md h d a , tinh toan gid tri PIC ( P o l y m o r p h i s m I m f o r m a t i o n C o n t e n t ) , eac gia tri ve ty le di h d p td, ty le khuyet sd lieu, He sd tfldng d d n g di t r u y e n (GS) dUde t i n h t h e o h e sd Jacard (Lanza etai, 1997).
81
: ' . etNam-S64(i!
2.3. Thdi gian \a dia diem nghien cdu Thi nghiem ddnh gia da dang di truyen 24 ddng ngd ddn bdi kep bdng ehi thi SSR dUde thflc hien vao vuXudn nam 2017 tai \ien Nghien edu Ngd.
in. KET QUA \ A THAO LU.\N
3.1. Ket qua khao sat 23 mdi SSR tren 24 ddng ngd Qua bang 3 eho thay sd alien xuat hien eua
cac mdi dao ddng td 2 den 5 alien, trong do mdi phil01049 (Hinh 1) va mdi umcl399 Id mdi ed sd alien xuat hien nhieu nhal tdi 5 alien, cd 5 mdi eo sd alien il nhat la (2 alien) dd la mdi phil00175, phi78.
umcl 196. phi448880. ume2359 eac mdi eon lai eo sd alien dao ddng td 3 - 4 alien. Tdng sd alien the hi?n tren 23 mdi SSR nghien cflu Id 70 alien, trung binh sd alien xuat hien Id 3,04 allen/mdi.
T T 1 7 3 4 5 6 7 8 9 10 11 12
Bang 3. So alien, he so PIC
Ten moi u m c l 399 phi374118 phii 12 phi 100175 phi452693 phi233376 phi423796 phi78 phi84 u m c l 161 phi 127 u m c l 196
So allele
5 3 3 2 3 4 3 2 3 4 3 2
TV le khuyet so lieu (%)
3,33 3,33 3,33 0.00 3.33 0,00 0,00 0,00 0,00 0,00 0,00 0,00
va ty le khu) He so
PIC 0,76 0,42 0,58 0,38 0,36 0,47 0,29 0,50 0,35 0,57 0,60 0,28
et so STT
13 14 15 16 17 18 19 20 21 22 23
leu cua 23 m o Ten moi phi93 u m c l l 0 9 u m c l 3 0 4 u m c l 2 7 9 u m c l 136 phil01049 phi448880 u m c l 153 n c l 3 3 umc2359 u m c l 8 6 2 Trung binh
SSR diing So allele
3 3 3 3 5 2 3 3 2 3 3,04
c i b o l Ty le khuyet
so lieu (%) 0.00 6,67 0,00 6,67 3,33 0,00 0,00 0,00 0,00 0,00 0,00 1,30
He so PIC 0,53 0,46 0,43 0,31 0,48 0,68 0,38 0,58 0.43 0,50 0,59 0,47
3 1 1 bp —
5 17 18 19 20 21 22 23 2 1
249 bp —
Hinh I. Ket qua di san pham PCR moi phil01049 cua 24 ddng ngo tren gel polyaerylamide 4,5%
Quan sat hinh anh tren bdn gel dien di sdn pham PCR mdi phil01049 eho thay locus cd 5 alien vdi 24 bang DN-\. Tat ea eac ddng deu xuat hien 1 bang duy nhat. \'di mdi phiI01049 thi cac ddng deu the hien ddng hdp td khdng xuat hien bang khuyet sd lieu hay di hdp tfl.
- Ty le khuyet sd lieu: Bang 4 cho thay ty le khuyet sd lieu cua cac mdi thap chi ed 2 mdi umcl 109 vd umel279 ed t>- le khuyet ldn hdn 5% (6,67%). Ty le khu> et sd lieu trung binh Id 1,30% va tat cd khuyet sd lieu eac mdi deu cd ty le nhd hdn 15% dam bao yeu eau cho cae phdn tich tiep theo.
- He sd PIC: Ket qua d bang 4 cho thay gid trj PIC bien thien tfl thap nhal la 0,28 (mdi umcl 196) den cao nhat Id 0,76 (mdi umcl399) vagid tri trungblnh la 0,47. Ket qud nay thap hdn ket qua nghien cflu ciia Bantte vd Prasanna (2013) cd he sd PIC trung binh Id 0,54; nghien cflu cua Pandey va Hossain (2015) he sd PIC trung binh Id 0,50 vd he sd PIC trung binh (0.48) trong ket qua nghien cdu cua Krishna vd cdng tac vien (2012). NhU vay vdi he sd PIC nhu tren eung chflng td bd mdi sfl dung trong thi nghiem de ddnh gia cdc ddng chfla da dang. Trong so eac mdi nghien edu dd thi cd 2 mdi ed gia tri PIC cao nhfl umcl399 (0.76) va phil01049 (0,68). Nhu vdy cd the
Tap chi Khoa hoc Cong ngne i\dng nghieo Viet Nam -564(113)/2020 thay cd eac ddng ngd cd sU da dang rat cao, dd Id
nhflng ddng cd Uu the trong cdng tac lai tao gidng.
Ben c^nh dd. cdc mdi cd gid tri PIC thap nhfl mdi umcl 196 va phi423796 (0.28 vd 0,29) the hien sU phdn bi?t gifla eac ddng.
Ket qua dien di mdt so mdi dien hinh dflpc trinh bay trong ede hinh 2 vd hinh 3 ngd thuan eda mdi thap, khdng nen sfl dung cho nhflng nghien cflu tiep theo.
1 2 3 4 5 6 7 8 9 l O 11 :2 13 1 4 IS I S 17 1 8 19 2 0 2 1 2 2 2 3 2 4
Hinh 2, Ket qua di san pham PCR mdi ncI33 cua 24 ddng ngd tren gel polyaerylamide 4.5%
151 bp 140bp l i s bp 100 bp
M 1 2 3 4 5 6 7 8 9 lO 11 12 13 14 15 16 17 18 19 20 21 22 23 24
Hinh 3. Kel qua di sdn pham PCR nidi umcl862 cua 24 ddng ngo tren gel polyaerylamide 4.5%
Quan sdt hinh anh tren ban gel dien di sdn pham PCR mdi nel33 (Hinh 3) cho thay locus cd 2 alien vdi 25 bang DNA. Mau D17 cd 2 bang xuat hien the hi^n trang thai di hdp tfl.
Quan sdt hinh anh tren ban gel dien di san pham PCR mdi umcl862 cho thay locus ed 3 alien vdi
24 bang DNA. Tat ca cdc ddng deu xuat hien I bdng duy nhat. Vdi mdi philOI049 thi cdc ddng deu the hien ddng hpp td khdng xual hien bdng khuyet sd lieu hay di hdp tfl.
3.2. Do thuan di truyen eua 24 ddng ngd nghien cdu le khuyet sd lieu (%M) va ty le di hdp tfl
Bang 4. Ty . ^ . , , , . . ,
„,, ... Tyle So mdi „ , , , , . _. So mdi , . ' • ,^ Ty le di
™„ Ten , , ,^ khuyet xuat ' , „ _ Ten I STT ,, khuyet ^,/. , . , .. hdptu STT ,, I done ..,/, solieu hien di ,„,, dong
"'^""' (%i hop tfl <">
I 6
i 7
8 9 10 ' 1112 13
(%H) 24 dong ngo cua bo I
DI D2 D3 D4 D5 D6 D7 D8 D9 DIO Dll D12 D13
0,00 0,00 0,00 0,00 4,35 0,00 0,00 0,00 0,00 0,00 0,00 4,35 0,00
0,00 0,00 0,00 0,00 0,00 0,00 0,00 0,00 0,00 0,00 0,00 0,00 0,00
Tyle So moi ,„. So moi , . *.
Ten , , , khuyet xuat done , , . , solieu hiendi solieu ,„,: . • ...
'"' ^ hop tu
19 20
23 24
D14 D15_
D16 D17 D18 D19 D20 D21 D22 D23 D24
1
Trung binh 0 0,38
(%)
0,00 4,35 4,35 0,00 0,00 0,00 8,70 8,70 0,00 4,35 0,00 1,63
0 2 0 0 0 0 0 0 0 0 0,83
Tyledi hop tit
(%)
0.00 0,00 9,09 0,00 0,00 0,00 0,00 0,00 0,00 0,00 0.00 0,38
^ap chi Khoa hoc Cc- g nghe Nong nghiep Viet Nam - So 4(113)/202C Ket qua bang 4 cho thay cac ddng deu cd ty le di hpp td thay ddi tfl 0 den 9,09%. Gia tri trung binh eda ti le di hdp id Id 0,38% the hien cdc ddng ngd ed dp thuan rat cad tren cae locus khdo sat. Ty le khuyet sd lieu d cac ddng ngd khd thap. 5 ddng Id D5. D12, DI5, D16, DI ed mpl mdi khdng dfldc khuech dai tddng dng vdi ti le khuyet sd lieu la 4.35%, hai ddng D20 va D21 cd 02 mdi khdng dddc khuech dai vdi ti le khuyet sd lieu efla ddng cad nhat la 8,70%. Ty le khuyet sd lieu trung binh cua 24 ddng Id 1,63% va d mde rat thap vd thap hdn 15°o, vi vdy sd lieu cau 24 ddng ngd dam bdo do tin cay khi sfl dung de phan tich.
3.3. Phan nhdm da dang di truyen 24 ddng ngd bdng 23 moi SSR
Kel qua bdng 5 cho thay he sd Ifldng ddng di truyen cua cac ddng bien thien trong khodng tfl 0,08 - 0,76. Nhin chung, khoang each di truyen efla eac ddng tddng ddi Idn cho thay cac ddng tUdng ddi khde biet nhau ve vdt chat di truyen. Day la mdt trong nhflng thuan Idi Idn ddi vdi ehpn tao gidng
ngd lai. Id ed sd de cd dfldc nhdng td hpp lai eo kha nang ket hpp cad hay ndi each khde la eo bieu hien Uu the lai eao.
Ket qua phan nhdm di truyen theo phUdng phap UPGMA (Hinh 4) cho thay d h? ^d tfldng dong di truyen 0,30 cdc ddng ngd chia ldm 2 nhdm ldn:
Nhom ldn I bao gdm 21 ddng, bao gdm: DI, D2, D12. D14. D15, D3. D4. DI6. D17. D5, D6. Dll, D21, D22, D13, D8, DI8. D20. D19. D23. D24;
Nhdm Idn II bao gdm 3 ddng: D7, D9, DIO.
Nhdm Idn I d he sd tUong ddng di truyen 0,32 dupe chia thdnh cdc nhdm nhd: Nhdm thfl cap 1.1 bao gdm 15 ddng: DI, D2, D12. D14, D15. D3, D4, D16. D17, D5, D6, D l l , D21, D22, D13; Nhdm thfl cap 1.2 bad gdm 6 ddng: D8, D18, D20, D19, D23. D24.
CJ thi nghiem nay, da sd ede ddng tham gia ddnh gid ed khoang each di truyen tUdng ddi xa (ngoai trfl cap ddng D2-D12 va cap D23-D24) do dd khd ndng tao dUdc con lai ed flu the lai cao la rat ldn (Bang 5).
-D16 -D17 -D5 -D6 - D l l -D21 -D22 -D13 -D8 -D18 -D20 -D19
|D23
Hinh 4. Sd do phan nhdm da dang di truyen cua 24 ddng ngd cua bp I dfla tren 23 mdi SSR theo phUdng phdp phdn nhdm UPGMA
Tap chiKhoa hgc Cong nghe Nong nghiep Viit Nam - 564(113)/2020
I 2 \
i Q I o o c o
— O O O O O O O l
o o o o r^ c^ o (N IN -^
o o o o o o o o
o o o o
, 0 0 0 0
0 0 0 0 0 0 0 0 0 0 o o
0 0 0 0 0 0 0 0 0 0
— 0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
-H o o o o 0 0 0 0 0 0 0 0 0 0 0 ,
0 0 0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0
O ^ — ( N r - l C N - ^ t N l N T t - f - i r ^ p tNir-, — ro
— ^ o d o ' d o o o o o o o o o o d 0 0 0 0 0 0 0 0 0 O O ^ ^ ^ O ^ C p O T ^ • - H G O ' ^ ' N C ^ " ^ < N
~ : : 5 c 5 c i S : : i c 3 C : < : 5 < : 5 < : 5 a c i
• „ l o
o o o o o o o o i o o 0 0 0 0 0
; - - o o o o o o o o o o o o o o
-If —• i n r i
d o d o
O • * — f, IT, d <:i tzi 1:6 o
0 0 0
IV. KET LL AN V \ DE NGHI 4 . 1 . Ket l u a n
Q u a n g h i e n e d u c h o thay 24 d d n g n g d d d n bdi kep t r d n g n g h i e n e d u d e u cd d o t h u a n di t r u y e n k h d c a o (cd ty le di h d p td n h d h d n 10%). H e t h d n g m d i SSR sd d u n g t r o n g thi n g h i e m tUdng ddi da h i n h voi gid i n PIC t r u n g b i n h dat 0.47.
P h a n n h d m Uu the lai t h e o phUdng p h a p UPG.MA d b d I e h o thay: d h e sd lUdng d d n g di t r u y e n 0,30 cae d d n g ngd chia lam 2 n h d m ldn (21 d d n g vd 3 d d n g ) . O h e sd tUdng d d n g di truyen 0,32 n h d m ldn I d d d c chia t h a n h 2 n h d m thfl c a p (15 d d n g vd 6 d d n g ) . 4.2. D e n g h i
T r d n g thdi gian tdi tiep tuc d d n h gia, dac d i e m n d n g sinh h p c , k h a n a n g ket h d p cua tap dddn d d n g ngd d d n bpi kep p h u c vu c h d ehfldng t r i n h c h p n tad gidng ngd lai c u a \ ' i e n .
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Nguyen I h i Lang, Bui Chi Bdu. 2005. Si>i/i hoc phdn tii: Gidi thieu phiidng phdp vd Ung dung. NXB Ndng nghiep thanh phd 116 Chi Minh.
J o h n L P o r t w o o d . i l , M a r g a r e t R W o o d h o u s e . E t h a l i n d a K C a n n o n , lack M Gardiner, Lisa C Harper, Mary L Schaeffer, Jesse R Walsh, Tancr Z Sen, Kyoung Tak Cho, David A Schotl, Bremen L Braun, M i r a n d a Dietze, Brittney Dunfee. C h r i s t i n e G Elsik. Nancy M a n c h a n d a , Ed Coc. Marty Saehs, Philip Stinard,
Josh Tolbert, S h a n e Z i m m e r m a n , C a r s o n M .Vndorf, 2018. MaizeGDB 2018' the maize multi-
^enorae genetics and genomics database. Nucleic Achb Research, Volume 47. Issue D I . Pages D 1 1 4 6 - D U 5 4 .
A M B I O N E T - Asian Maize Biotechnology Network, 2004. Laboratory handbook: Protocol for maize genotyping using SSR markers and data analyi^is.
AMBIONET Service Laboratory, CIMMYT.
Bantte K., P r a s a n n a B.M., 2013. Simple sequence repeat polymorphism in Quality Protein Maize (QPM) lines. Euphytica, 129; 337-344.
Lanza L, L, B, C, I, J, Souza, L, M, O t t o b o n i . M, L, C, Vieira, A, P, Souza, 1997, Genetic distance of inbred lines and prediction of maize single cross performance using RADP maker. Tlieor. AppL Genet 94:1023-1030.
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Saghai-Maroof M.A., Soliman K.M., Jorgensen R.A., Allard R.W., 1984. Ribosonial DNA spacer-length polymorphisms in barley: Mendelian inheritance.
chromosomal location and population dymnamics.
Proc. Nali Acad. Sci. USA. 8 1 : 8014-8018.
Genetic diversity evaluation of 24 double haploid maize lines created by haploid inducer technology
Nguyen Due 'Ihanh. Dang Ngoc Ha, Nguyen Van Truong. Nguyen The Hung Abstract
In recent years, application of haploids and double haploid lines has achieved many successes in maize breeding of \'iot Nam. With the helping of CIMMVT. the National Maize Research Institute of Vietnam (MRl) has applied successfully the haploid inducer teehnolog)'. Twenty four DH lines were evaluated for genetic diversity by 23 SSR markers in the spring season 2017. The evaluation result showed that the genetic purification was high and the homozygous ratio reached more than 90 percent. 24 DH maize lines in this study were divided into two major groups (twent)' one and three lines) at 0.30 genetic similaritv- coefficient. The major group one was divided into two secondary groups (fifteen and six hnes) at 0.32 genetic similarity coefficient.
Ke^-words: Double haploid lines, double haploid technology, genetic diversity Ngay n h a n bdi: 10/4/2020
Nga\ phdn bien: 2 6 / 4 / 2 0 2 0
NgUdi p h a n bien: T S Mai Dflc C h u n g Ngay duyet d a n g : 2 9 / 4 / 2 0 2 0