HOl NGH! KHOA HQC C G N G N G H E SINH HOC T O A N Q U O C 2013
NGHIDN CLTU O'NG DyNG VI KHUAN PROBIOTICS TRONG SAN XUAT NUdC cA R6T LEN MEN
' Nguyen Thj Hgnh'', Nguyen Thj Thanh ThOy\ Hodng Th| Lf Hing*, Nguyin Thi DiSu T h i i / —
^Tntdng D?l hgc Ndng nghi$p Hi Ngi 'Vi^n Nghien cdv rau qua
T6MTAT
Nude qud probiotics dang du^c phat trien trong th6i gian gin day ciing vdi moi quan iSm ngay cing lijn den svtc kh6e ctia ngtifri ti&
diing. NghiSn ciiu nky dirge thyc h i ^ nham dinh pi sif phii bijp ciia mroc cd r6t ikm nguyen lifu cho v i ^ s4n xu4t nude qui probiolics vdi ba chiing vi khuln Lactobacillus acidophilus HT, Lactobadllus fermentum HA6, vk Bacillus clausi. Cic chiing vi khuan du^c bo sung vao mroc ca rdt v6i nong d$ 10* CFU/ml. Sv thay ddi vl so lugng t^ bao vi khuln song trong nude cdr6t, chit lugng cam quan ciia nude ca rSt theo thfri gian bio quin d 4° C dugc phin lich. Kit qna cho thiy Lactobacillus acidophilus HT cd kM i^ng song t^t ham hai chiing cdn 1^ trong nude ci r6t Lactobadllus acidophilus HT sau do dugc sii dpig lam chung gi6ng di len men nude ca r& a 37"C vdi ^ 1? 1,3, 5,7% (v/v) (m|t dd >10'CFU/ml) so vdi th^ tich nguyen hfu. K^ qui cho thiy nude ca ro^
1^ men bang Ladobaallus addophilus HTvdi n6ng d$ 3 vi S^ sinh kh6i d 37° C trong 24 h cho sin phim midc ci r^t probiotics cd chit lugne cim quan tot Tuy nhign, chi mau dugc b6 sung sinh kh6i vi Idnian nay vdi ndng dg 5% dim bio lirgng t^ bio s^g bong nude ci rot sau 40 ngay bio quin d 4° C Ii >10'CFU/ml. K^ qui budc diu cho fliiy, nude ci rot phii hgp cho sin xuit san phim do uong probiotics.
Tir Idioa: probiotics, nude ca rot probiotics, Lactobadllus acidophilus tiCfOKM
NhOng thip ky gan day, khi dd'i song x3 h^i ngdy cdng dirg'c ndng cao thi moi quan h | giira eke hi^i th^c ph&n vd si>c khde cHa ngu'&i tifiu diing cdng dupe quan tdm sJSu sac. Vi§c phdl trien cac logi thi/c pham vi>a giOp ndng cao si>c khde con ngu'iri, vtra cd tinh tidn dyng dang Id mijt trong nh&ng i/u KSn nghiSn ci>u cOa ngdnh cOng nghi§p thi/c phSm cdc nu-dc. Xu hu'ong ndy da vd dang klch c^u tidu thg cdc lo^i Ihi/c phSm gidu cdc Ihdnh ph4n chi>c ndng nhu* preblotic, probiotics, vitamin, khodng chit, cdc logl d^u cd vd cdc sterol thi/c vdt.
Vi§c si> dyng cac vi khuln probiotics trong chk biln thi/c phlim nhlm mgc dich cai thl^n si>c khde ngiz&i «Su dCing dd du'pc thyc hi|n trong khodng 20 nam qua (Krasaekoopt et al., ZiXiT). Probiotics duvc dinh nghTa Id nh&ng vi khuSn cd Igl s6ng trong difdfng HSu hda. Chting di/grc m$nh danh Id "vi khuln tot bgng" vl chiing giiip ca Ihl chfing igi mdt s6 vl khuln c6 hgi, nim vd sieu vi. Theo to chi>c Y l l ThI gidi, Ihg^ phim probiotics dug'c djnh nghTa Id cdc skn phim thg'c
• - phim c6 chi>a vi sinh v|t sing vdi lu'gng t l bdo du dam bao mang Igi Igi I'di cho s&c khde (FAO/WHO, 2001). Thi/c phSm pnabiotics cd the dem igi nhilu Ig-I Ich bao g6m si/ phdng ch6ng vd ch&a cdc bdnh dirdng mOt, kilm sodt mi>c cholesterol treing huyet thanh, tdng cirimg hk thong miln djch, cdi lhi§n khd ndng hip thg dirirng lactose vd phdng ch6ng hogt dgng ciia cdc hldrocacbon gdy urig thir (Mc Naught et MacFle, 2001). Hidn nay, Sii/c phim probiotics sdn xu^t theo quy md cdng nghiep dhii ylu Id cdc sdn phlrn tir sJ>a nhir sQa chua, sua chua u6ng. Tuy nhiSn, vi$c khfing dung ngp lactose vd s^r cd mgt cOa cholesterol trong s&a Id hal yeu t l hgn chl m&c tieu thy ciia ngu«i tidu dung d6l vdi cdc san phim ndy.
Niroc qud Id mgt trong nh&ng sdn phim d6 uAng dang ngdy cdng ttd nSn ph6 biln vd dirpc s& dyng hdng ngdy d nhilu nif dc, thay thi ddn- cac loal nirdc uong cd ga vd cdc logi nirtcuOng pha chl khde Nu'd'c t^ud khdng chi hip din nguin tidu diing'bdi hutng vi, mau sac md cdn cung d p nhilu vitamin, muoi khodng vd cdc vi chat dinh dirong d n tWil cho si>c khde. Gin day, nirdc qud dirgrc nghidn ci>u cho thiy Id mdi tnr&ng Ihlch hgp cho si/ phdt triln cua vi khuln probiotics (Maltila et al 2002). Mdt so sdn phim nu'd'c qud pnjbiot'cs cikig da dirge ngudn Ueu diing ddn nhdn (Krasaekoopt et Kitsawad. 2010) Trong sin xult nude qud pnsbiotics len men xdy ra' qud tinh Idn men cOa vi khuln probiotics, ldm tdng sinh khoi cua vi khuan vd sinh ra cdc axil hOu co- ti/ nhidn. tgo ra hirong v[ mdi ch9 san phim. Tuy nhiSn, vi$c kilm sodt nhiSt dO thich hpp cho qud trinh len men vd trdnh sy Idn men ti/ nhien cua cdc tgp ndm, lap khuln Id ydu d u khit khe.
Vdi khf hdu nhi#t ddi, thdi tilt ndng, ddn sfi Igi ddng (ho'n 80 tri§u ngirdi) nSn nhu d u v l nude gidi khdt d nude ta Id Mid Idn Hon nOa, do Id nude nhift ddi gid mOa ndn Vidt Nam cd ngu6n rau qud rlt phong phii. Trong sfi dd, ed rfit Id loai rau oidu dinh dudng vd tuo'ng dfii re. Nude cd rfit chua hdm lugrng Idn p-carotene, wtamin nhdm B, kali, canxi, coban vd nh&ng khodng chit bfi dudng khde. Cdc thdnh phln dinh dudng nay lam tdng cam gidc dn uing vd tdng khd ndng chfing Idy nhilm vi khuln (Nguyin Thidn Ludn et al., 1999). VI vgy viec chl biln nude cd rfit nhlm tdng gid tri thuong phim ciJa logl nguydn li#u ndy Id thgc sg d n thilt.
- NGUVeN Lieu VA PHUONG PHAP Nguydn lifu
CA rdt • Cd rfit gifing Nhdl trfing d xd Due Chlnh, Cim Gidng, Hdi Ducrng dugrc Iga chgn d l chl biln nude ek rfit Theo nh&na nghidn cdu oia Vifn nghidn cdu Rau qud, gifing cd rfit ndy dugc trong pho blln d mien Bac nude ta, qud cd mdu sic dep, miii vj ddc trung vd eho t^ 1$ thu hfii djch qud cao. Cd rot duge mua v l nlu chua si> dyng din dugc chiing tdi bdo'quan trong tiil LOPE d nhift d^ 2''C.
Ladobadllus acitftipMus ,HT: Thufic tfp dodn gifing cua Vifn cdng nghf sinh hgc, Vifn Han Idm Khoa hgc vd Cdng nghf Vift Nam.
HOI NGH! KHOA HQC CONG NGHE SINH HOC T O A N QU6C 2013
Lactobacillus femientum HA6' Thufc ldp dodn giong cua Bo mdn Cdng nghf Idn men, Vifn Cdng nghf sinh hgc vd Cdng nghf thgc phim, Tnrdng Dgi hgc Bdch khoa Hd Nfl.
Badllus dausii: Chung Ihuung mgi, xuat xu td Cdng ty Sanofi - synlhelabo. Ltd - Phap.
Phucng phdp nghidn ci>u
Chi biin nude ck rdt: Nude cd rfit dugc chl biln theo quy trlnh tham khao Id Vifn nghlSn cdu Rau qud, vdi t^ If phfii chl nguyfin lif u/nude Id 1/3, hdm lugng chit khd hda tan tdng sfi (TSS) dat 12° Bx.
Phtrcmg phip nudi dy vl slnh vit: Tu gifing ban dau dugc gid trono glycerin d dieu kifn -70° C, ede chung vl khuln dugc hogt hda lln thd nhlt trong mfli Irudng MRS Idng d dilu kifn 3 r C, lie nhg trong 24h. Sinh khfii vi khudn dd hogl hda lln mfl duge dua vdo djch nudi d y dd chufin bj vdi t j If 10% d l hogt hda lln hal. Sau 24 h nudi d y d 37 C, thu sinh khfii bing cdchiy tdm 7000 v/p trong 10 philt d 4°C.
Ura chgn chiing vl khuin Oilch nghl tren mdi tnrdng nude ci rdt: Bfi sung sinh khfii cda ba chdng vi khuln nghifin cdu vdo nude cd r« vdi nfing df ban diu khodng 10* CFU/ml. Theo ddi sfi lugng t l bdo vi khdn rrobiotics sdng tong 1 ml nude cd rfit, chll lugng cdm quan cOa nude cd rfit sau 10 ngdy, 20 ngdy, 30 ngdy, 40 ngdy, 45 ngdy bd sung vdo sdn phdm.
Phuong phip tin men nutSc cd nit Nude ed rfit sau kN phfii che dugc thanh triing d 15-15-15/8^ C. Qud trinh Idm ngufi dugc thgc hifn trong dilu kifn vd triiriQ d l dua nhift df cila nude cd rdt v l khodng 37° C. Sau dd, qud Wnh len men nude cd rfit dugc thgc hifn bing chiing vi khuln probiotics dd Iga dign dugc. ThI nghifm dugc thgc hifn trong cdc chai Ihl tlch 100 ml vdl d c IJ If d p gifing vi khdn probiotKs Id 1,3, 5,7% d nfing df 10^ CFU/ml. Trong thdi gian Idn men (36h), theo d6i sd lugng vi khuln Inang nude cd lit, hdm lugng chit khd hda lan tfing sfi, hdm lugng axit tfing sfi, pH djcrfi qua, chit lu-gng cdm quan ciia nude cd rfit, di Iga chgn 1^ If tilp giong phii hgp. Sau dd, Iheo ddi ehll lugrng sdn phim trong thdi gian bdo quin d 4° C
Xdc dinh him luvng chit khd hda tan tong s6: su dyng chilt quang k l hifn sfi Digital Refractometer PR-101 eiia hdng Atago (Nhf t Bdn) cd dll gidi hgn (0-45)" Brix, df chlnh xdeO.I.
Xic djnh pH bing miy dopHh/ d^ng
Xic djnh hkm tugng axit t6ng s6: Su dyng phuong phdp chuin df bing NaOH 0,1 N Xic dinh s6 li/gng ti bio sSng bong nude ck rdt- Sd dyng phuong phdp dem khuln Igc
Phucmg phip dinh gli cim quan: Chit lugng d m quan dugc ddnh gid thdng qua hfi dfing ddnh gid thj hilu (Hedonic scale) Iheo thang eho ffilm tu 1 - 9.
^uong phip xdtf'sd tiiu: Cdc sfi lifu thu duge td cde tht nghifm cua d l Idl dugc xd 1;^ bdng phln mim Excel 2007 vd chuong trlnh xd l<^ sfi lifu SAS 9.0.
K £ T QUA VA THAO UJ^
L\ra chgn chdng vl khuin thfch nghi tren mdi tnrdng ntedic ci rit
Sg ^leh nghl ciia cdc chung vl khuln probiotics tren mdi trudng nude ed rfit dugc ddnii gtd dga trdn sfi lugng vi khuln probiotics sfing trong nude cd rfit vd sg biln dfii chit lugng cdm quan eOa nude ed rfit trong thdi glan bdo qudn d 4° C.
Kit qud d Bdng 1 chl ra ring, cdc chung vi khuln probiotics song b'ong nude cd rot deu giim dan sfi lugng ttong Ihdi gian bdo quin.-d nhift df 4 - 6° C. Chiing fi. dausii kdm phdt triln nhlt trong mdi tnrdng nude cd rfit. Chiing L.
fermentum slnh trudng phdt triln tfit hon so vdi chung B. clausll vdi sfi lugng t l bdo vi khuln L. fermentum tgi d e Ihdi dilm phdn tlch diu cao hon B clausll d mdc y nghTa a = 0,05. Tuy nhlSn, sau 30 ngdy bdo qudn, sfi lugng vi khuln 6.
dausii vkL. femjenfurr? trong nude d rfit diu giim xufing mdc thap hon 10^ CFU/ml. Sau 45 ngay bdo qudn, sfi lugng vi khuln sfing trong nude d rfit ehi cdn khoing 6,3 x 10* CFU/ml (doi vdi B. dausii) vd 8,4 x 10* CFU/ml (dfii vdi L hrmentum) Chiing L. acidophilus thich nghi tfit nhlt trdn mdi trudng nude ed nSt, sau 45 ngdy bdo qudn d 4° C, sfi lugng vi khuln sfing cdn khodng 9,7 x 10* CFU/ml. L acidophilus eOng da dugc nghlSn edu cho thiy d khd ndng sfing cao trong nude ed chua (Yoon, et al., 2004/
Theo cdc nghidn edu v l thgc phim prtAiotcs. nfing df vi khuan d mdc 10^-10^ CFU/mi d thdnh phim dugc thilt Ifp nhu Id ngudng cd khd ndng mang Igi Igi feh eho sue khde con ngirdi (Harisk ef Varghese, 2006;,Sfi lugng vi khuln B.
daustivk L fermentum Irong nude cd rot diu khflng dat mdc ydu d u ngay sau 30 ngdy bio quin. Sfi lugng vi khuSn L.
acidophilus trong nude ed rot v5n dgt mdc Idn hon 10 CFU/ml sau 40 ngdy bdo quin.
Th&igian (ngay)
D 10 20 30 40 45
S6 lugrng t4 L addophilus
3,6* XlO*
5,0* X 10' 1,5' X 10' 6,1'XlO*
1,5" X l O ' 9 . 7 ' x l O '
bdo vi khu3n probiotics (CFU/ml) L. fermentum
3,8" X l O ' 3,7" X 10' 9.3'X 10' 7.5" X 10' 2,3'X 10*
8,4'XlO*
B. c/ausH 3,8'XlO' 2,8'X 10' e.A'xttf 5.1°X10*
9,2= X 10*
5,3= XlO*
HQ\ NGHI KHOA HQC C<!)NG NGHg S I N H ' H Q C T O S N Q U O C 2013
jTrf^rsj-r,ing-mfit-hABg-^ac-sAp.^i^fl ^fij ^jAng nhaii thi khdng khic nhau d mik
V l c h i t lugng cdm quan. Bang 2 cho thay nude d rfit bfi sung vi k h u l n L fermentum nhanh chong^co s g Dierrdottheo- h u d n g x l u ve trgng thdi, huong thom vd vj Irong thdi gian bao quan. D i l u ndy id do qud trinh trao dfii chat ciia chCing L.
fermentum ed sinh khi v d tao ra sdn p h i m phy ngodi ax i l lactic, lam thay dfii miii vn eiia n u d e d rfit Sau 30 ngdy, d i l m cdm quan v l trgng thai, h u o n g thum vd vj cua n u d e c d r ^ t)fi sung Ltormentum deu gidm xufing thap h a n 7,0. N u d e d . rot b d s u n g viJchuIn L addof^IIusyk B. clausll hit d i u c d s u thay dfii c h i t l u g n g cdni quan sau 40 ngdy b i o q u d n / T u y nhidn, sau 4 0 - 4 5 ngdy bao qudn, s i n pham van d u g e ddnh gid d mdc d f thieh ( d i l m cam quan tfing Kgp sau 40 ngdy dat 8,2 dfii v d i nude cd rfit bfi sung chiing L acidophilus vk 8,0 dfii vdi n u d e d rot bfi sung ehCing B. dausii). D i l m d m quan v l mdu s i c cua n u d e cd rfii bfi sung cdc chDng vi k h u l n probiotics d i u g i i m dan trong thdi gian bdo q u i n , nhung khong ed s g khde nhau gida d e d n g I h d c trong ciing m f t ttidi gian phdn tlch d mdc y nghTa a = 0,05.
T d nlidng k i t q u i cua thi nghifm, chung vi k h u l n L acldopt^us duge Iga chgn d l len men nude d rfit
Bang 2. Sg biftn dSi chat lugng cdm quan cila nude cd rfit trong th64 glan bao qudn d 4 * C khi bo sung cdc chiing probiotics
Thdi gian (ngdy)
0
10
20
30.
4D
45 ChOng vi khuln L acidophilus L fermentim B. dausii L addophilus L fermentum fl. dausii
> L addophilus L. fermentum B. dausii 'L aeidophSus
1-fermentum B. dausii L addophilus L fermentum
B. dausii' L acidophilus L. fermentum B. dausS
Trang thai 8,5°
.8.5"
8.4"
8,5' 7,6' 8,5"
8.4' • 7,2"
8.3' 6,5"
6.3' 8.1' 6,1"
7,9' 7,8' 5,9=
7,6°
Huong thom 8.4' 8.5' 8,5*
8.4' 7.5"
8.4' 8,6' 6.9°
8.2' 6.4*
6,5°
8.3' 7,9' 5.7*
7.9"
8,0' 5.5°
7.9' V[
8,8"
8,5' 8,6' 9.0' 7,9=
8,6"
8,8"
7,2=
8.5°
8,7*
6,7' 8,2"
8,3' 6.4"
8,1*
7.8"
6,2°
7.7"
Mdu s i c 8,7' 8,8' 8,8"
8,8' 8,7"
8.6' 8,7*
8.8' 8.6' 8.5' 8.4' 8,5' 8,4' 7,9' 8.0'"
8,0' 7.9' 7.9*
(Trong cOng m^t c0f, (31 ctirg mt}t thdi gian. cies6cdchir eSI giing nhau Oil khdng khic nhau d mde y nghTa a = 0.05) Xac d/nh (j^ / f tiep gidng vl khuin L. acidophilus cho qui trinh len men nw<5c cd rot
T9 i f t i l p gifing vi khuln L addophilus dugc thd nghifm Id 1,3,5,7% (v/v, m f t d f > l O ' CFU/ml) so vdl t h | tlch nude cd rfit.
Cde chl tidu t h i hifn d f n g thdi cOa qud trlnh len men dugc theo doi moi 12 h Idn men vd,augc t h i hifn d Bdng 3.
Bang 3. Anh hudng ciia ty l« t i i p gifing vi khuSn L. acidophilus den d^ng t h i i ciia qui trinh l i n men nude ca rot
Thdi glan TJ If Ign men tiip gifing
Chi tieu theo dOi Ti bdo vi khuln
(CFU/tnl) TSS (°Bx)
TiSp gifing 1%
f l i p gifing 3%
Ti4p gifing 5%
TiSp gifing 7%
6.5* XlO*
9.1'X 10'
0,041' 0.041' 0,041' 0.041' TlipgiSng1%
Tilp gifing 3%' Tifip gifing 5%
TlSp gifing 7%
2.4' X 10"
8,5" X 10' 3.0= X 10' 6,1'X 10'
10,5' 10,3°
10.2° - 10,0=
0,102"
0,111"
0,127°
5,03' 4,70' • 4,56°
Tiip gifing 1%
Tl4p gifing 3%
Tllp gifing S%
Tllp giong 7%
9.4'X 10' 6,6° X l O ' 2,5= X10' 7.4'XlO''
0,096' 0,120"
0,131"
0.142' 4,42"
4,26"' 3.97=
TiSp gifing 1%
Tl4p gifing 3%
TTlp gifing 5%
Tilp gifing 7%
1.6^x10' 9.5= X l O ' 6,5" X10"
1,5* X 10"
g.tf"
9,2=
8,7"
0.115=
0.132"
0,153' 0,161'
rf,f^cOngm^t cdt, ^icOngmCt Old ^an. cic s6cd chd cii giing nhau till Idling IdiSe nhau d mde f'nghia a = 0,(^
Theo ddi ddng Oiil cua qui trinh lin men cho kit qui nhw sau:
HOI NGHI KHOA HQC C O N G N G H ^ SINH HQC T O A N Q U 6 C 2013
Ve s6 lirgng vl khuin L addophilus: K i t qua d Bdng 3 cho I h l y vl khuan L. acidophilus Irong n u d e cd rfit d cdc cdng thuc d i u tdng sinh khfii trong (hdi gian ISn men. Sfi lugng vi k h u l n L acidophilus tang len r l t nhanh trong 24 h d a u ISn men. sau dd cd xu h u d n g tang chgm Igi. Sfi l u g n g vi khuan L acidophilus gida cdc cdng Oidc thi n g h i f m trong cdng thdi gian len men ed s g khde nhau hodn loan d mdc y nghTa a = 0,05. Sau 24h len men, sfi l u g n g vi khuan L addophilus trong n u d e cd rfit d ty I f t i p gifing 3% (v/v) dgt k h d n g 6,6 x 1 0 ' CFU/ml, d t? IS 5 % (v/v) dgt 2.5 x 10 CFU/ml (luong dng vdi sfi l u g n g vi k h u l n L acidophilus ban d i u bfi sung vdo nude cd rfit probiotics khflng Idn men), d ty I f 1 % ^v^) dgt 9,4 x 10* CFU/ml, d ty I f 7% (v/v) d^it 7,4 x 10* CFU/ml vd khflng tang len ddng k l sau 3 © ! ISn m e n . Vi him lugng chit khd hda tan tdng s6: T9 I f t l l p giong d n g cao thi hdm lugng c h i t khfl hda tan gidm xufing cdng nhanh do tfic d f Idn men Idn. Sau 24 h Idn men, hdm l u g n g chdt Wid hda tan trong n u d e cd rfit d cdc edng thde v e t j I f t i l p gifing l l n lugt Id 10,0" Bx, 9,8° Bx, 9,6° Bx, 9,2° Bx vd khong gidm n h i l u sau 36 h Idn men.
Vi hkni lugng axit hOu co tong s6: Dbng thdi v d i s u lang sinh khoi vi k h u i n Id hdm l u g n g ax i l h u u ccr trong n u d e ed rfit tdng ISq d t i t d cdc cfing thdc thi nghifm (sdn phSm eiia qud trinh iSn men), Sau 24h ISn men, hdm l u g n g axit hOu co- ifing sfi trong nude cd rot d ty I f t i l p gifing 3% (v/v) id 0,120%, d t j I f 5% (v/v) Id 0,131% v d khdng c d s g k h i c nhau d mdc y nghia a = 0,05 gi&a hai cflng thde ndy. N u d e cd rot d t j I f t i l p giong 7% (v/v) d hdm l u g n g axit hOu c o dgt 0,142% d 24h ISn men vd khdng sai khde d m d c i nghTa a = 0,05 so vdi d t j I f liSp gifing 5 % (v/v). Tgi Ihdi d i l m ndy, nude cd rfit d ty I f t i l p gifing 1 % (v/v) cd hdm lugng axit h&u ea t l i l p n h l t id 0,096%. Gia trj pH cua n u d e cd rfit d c i c cflng thdc d i u gidm di tirung dng vdi s g tang ISn eiia hdm lugng axil hdu ccr tfing sfi.
T * If s i p "gifing vi k h u l n L. addophilus vk thdi gian ISn men dnh h u d n g r l t idn d i n c h i t l u g n g d m quan ciia n u d e cd f o l , d i l u ndy d u g c t h i h i f n trong Bang 4 . Theo Monika ef al., (2005), c h i t l u g n g cdm quan cda n u d e tdo-cd rfit ISn m e n bdi chiing L. addophilus CH-2 d 37° C, c h l l l u g n g d m quan d a s i n p h i m dgt mdc ySu thich n h l t sau 20 h ISn m e n . Bdng 4. Anh hudng ciia ty 1$ t i l p gifing vl khuln L acidophilus d i n chit tugng c;
men
fl quan ciia n u d e ca rfit bx>ng qud trlnh Ifin
Thdi glan ISn men
Oh
12h
24h
36h T y l f tllp gilng Tllp gifing 1%
Tllp gilng 3%
Tllp gilng 5%
Tllp gifing 7%
Tilp gilng 1%
Tllp gilng 3%
Tllp gifing 5%
Tifip gifing 7%
Tifip g i ^ g 1 % Tifip gifing S'/D T l l p gifing 5%
Tilp gifing 7%
Tllp gifing 1 % Tllp gifing 3%
Tllp gifing 5%
Tllp gifing 7%
Trgng thdi 8.6' 8,5' 8,6"
8,5' 8,5*
8.4' 8.4' 8,5' 8,8*
8.7*
8,8"
8,8"
Chlti^ucin Huong thom
7,4' 7.3' 7,5"
7,3' 8,1' 8,2°°
8,4"
8,5' 8,3' 8,r 8,8' 8,7' 8.5' 8,4"
8,2°
8.0=
quan Vi 6,5' 6,6*
6,6' 6,7= <
7,1°
7,8"
8,5' 7,1"
8,2*
8,0' 8,7=
8.0"
7,9' 6,9'- 5,8'
Mdu sdc 8,8*
e.r 8,6'
" 8,8" - 8,7*
8,8' 8,6' 8,6' 8 , r 8,6' 8,6' 8,5*
8.6' 8,5"
(Trtmg cung mft c{U, t^i cOng m^ Old gian, cic s6 cd diCrcii giing nhau Oil khdng khic nhau d muc y nghTa a = 0,05}
Bing 5. Sg biln dfii sfi lugng vl khu^n L. acidophilus vd m^t GO chl lieu h6a hpc ciia nude cd rfit l l n men trong t h d i glan bdo q u i n d 4 - 6 ' C
Thdi BQ (ngdy)
0
40
T l bdo vl khul (CFU/ml) 7,6' 5.4=
3.6"
x 1 0 ' Ty I f tilp gilng
TSS (PBX) 9,9°
10,0' 9,9"
9,9"
3%
AxllTS 0.121''
(%)
0.128"
0,133°"
0,140"
0,146' 0,150'
pH 4.47"
4,36' 4,19' 4,01=
3,92"
3,86'
Ty If tilp gifing 5%
T i b d o (CFU/ml) 5,5' 1,4' 3,4=
6,4"
1,7*
6,1 x l O ' xin' xin' xin' xin' xio-*
- (°Bx) 9.6' 9,5' g.e"
9,5*
9.5"
0,133"
(%)
0,138"
0.142"
0,154"
0.159' 4,25' 4,11' 3,97°
3,91°' 3,82"
3 . 7 r (Trong cOng mpt eptcScsSed chO- oil giing nhau thi khdng kh^e nhau d mOc ^ nghTa a = 0.05) ' D i l m d m quan d u g e thfing ke Igl d B i n g 4 cho t h i y qud trlnh ISn men tdc d f n g n h i l u nhat d i n vj vd h u o n g thom eOa s i n p h i m . Qud trlnh ISn men tgo ra huong thom mdi eho sdn p h i m . Vi vfy, d i l m d m quan v l huong thom eOa s i n p h i m d t i t d ede cdng thdc dfiu taiw d i n Irong nhdng gid d i u len men, sau dd gidm di do sdn p h i m len men ldm m i l hucmg t h o m t g nhiSn eiia nude cd rfit D i l m d m quan v l vj cua s i n p h i m tdng dan trong thdi glan ISn men do vi k h u l n probiotics s d dyng dudng Idm ca c h i t cho qud trinh ISn men, tgo ra axit h d u c o Idm cho h u o n g vj ciia san p h i m d vj chua nggt hdi hda. Sau 24 h ISn men, nude ca r l t d ty I f d i p gifing 3% v i 5% cd d i l n i d m quan v l h u o n g thom v d >^ cao n h l t . T u nhung nhfn xdt tren, ehiing tfli Iga chpn n u d e cd rfit idn men cd ty I f fiep gifing 3% vd 5% sau 24 h ISn m e n d l C leo
HOI NGH! KHOA HQC CONG NGH£ SINH HQC TOAN QUOC 2013
~~d6rsg-bierrdfirsfi-|ugng-vrkhulrrfc-acKtop/w/os-vd TTIfts6-chf-ti§H-tida-hpcrehat-lucrng-dnrquan trong-thdi-gian bao—
q u d n d 4 - 6 " c .
Ket q u a t h e o doi s g b l l n d f i i s f i l u g n g v i k h u l n L, acidophilus t r o n g n u d e ca rfit vd m f t s o c h i t i i u hda hgc eiia n u ^ c ed r o t theo t h d i glan bdo q u d n d u g c t h i h i f n t r o n g Bang 5
S g b i l n doi s f i l u g n g t l bdo vi k h u l n sfing' trong n u d e ' cd rot Id y l u tfi quan 'trpng n h l t trbng t h d i 'giari"bSo quan s i n p h i m . S g sfing ciia v i k h u l n p r o b i o t e s p h y t h u f c v d o s u cd m f t eiia oxy trong sdn p h i m , c h l l l u g n g cOa b a o bl bao q u d n , t h d i gian ISn men vd n h i f t d f ton I r u . S g song cda vi k h u l n probiotics c i i n g phu t h u f c v d o ede c h i t d c c h l n h u hdm l u g n g acid d u g e Igo ra t r o n g t h d i gian bao qudn ( Y o o n , ef al., 2005). K i t qud d Bdng 5 eho I h l y sfi l u g n g vi k h u l n L. acidophilus gikm d i n trong thdi glan bdo q u d n . N u d e cd rfit len men t i l p gifing 5%
- g i d d u g c so lugng vi k h u l n L. acidophilus dgt yeu d u d i n 40 ngdy b i o q u i n , vdi sfi l u g n g vi k h u l n khodng 1-7x10*
CFU/ml. Ve mgt hda hgc, hdm l u g n g e h l l khfl hda tan Irong n u d e cd rfit g i n n h u khdng ed s g b i l n dfii d i n g k l trong sufit thdi gian bdo q u i n . Ham l u g n g axit h d u c o trong n u d e ca rfit ISn men tang nhg theo thdi gian bdo qudn do qud trlnh trao doi c h i t
T u nhOng phan lich v l s g bien doi sfi l u g n g t l bdo vi k h u l n sfing trong nude ed rfit vd c h l l l u g n g cOa sdn p h i m trong thdi gian len men, thdi gian bao q u i n , chiing 161 k i t ludn I j I f fiep gifing vi k h u l n L acidophilus phit hgp cho qud Irlnh I f n men n u d e cd rfit Id 5% d l d i m b i o sfi l u g n g vi W i u i n L addophilus trong nude d rot dgt trSn 10* CFU/ml vd duy Irl d u g c c h i t l u g n g cdm quan cQa san p h i m sau 30 - 40 n g i y b i o qudn.
K £ T LUJBIN
Chung vi k h u l n Laddophilus d kha nang Ihlch nghi tfit n h l t Irong mdi trudng nude cd rfit so vdi hai chGng cdn igi vd khdng i n h hudng x l u d i n chit lugng earn quan cOa nude ca rfit khi bfi sung. Khi dUgc dua vdo nude ed rfit vdi nflng d f ban d i u Id 10^
CFU/ml, sau 40 ngiy b i o qudn d 4 ' ' c , sfi lugng vi khuln sfing v i n (fet> I t f CFUMil
T J I f t i l p gifing vi k h u l n L. acidophilus phii hgp cho qud trinh ISn men nude c i rfit i i 5% vdi sinh khfil vi k h u l n ban dku dgt 10^ CFU/ml. Thdi gian ISn men tfit n h l t id 24h d l ddm bdo sfi l u g n g t l bdo vi k h u l n L acidophilus trong s i n p h i m vd e h I t l u g n g cua sdn p h i m n u d e d rfit probiotics ISn m e n . Khi dd, nude d rfit iSn men probiotics d s o l u g n g vi k h u l n L acidophilus sfing dgt >10* CFU/ml. Sau 40 ngdy bdo quan d 4°C, sfi l u g n g vi k h u l n ndy sfing trong sdn p h i m vSn dgt n g u d n g y S u d u (>10^ CFU/ml), dam bdo kha ndng mang Igl Igi Ich eho sdc khde con ngudi.
TAI LI^UTHAMKHAO
1.1 Thj Lan Chl (2010). Nghidn pdu edng nghi sin xuit chi phim pn±lotics (visinh'v$t c6 Ipl cho sdc khde) chit lugng eao vi dng dung cho cic Oivcphim chdc nSng BSo cdo 3e tdi rap Bf .Vif n cong n ^ f sinh hoc - ding n ^ $ thgc phim.
Hodng Thj Lf Hing, NguySn Vdn Quylt. Nguyfin Tlii Hiln (2006). Nghien ci>u cdng ngh$ sSn xult nude cd rfit d Vift Nam. Tfip chi DD&TP/Joumai of Food and Nutrition Sdences -T|p 2 - s l 2 - Thdng 7 ndm 2006.
FAO/WHO. 2001. Expert Consultation on Evaluation of Health and Nutntiond Properties of Probiotics In Food Includldg Powder Milk with Live Lactic Add Baderia.
Harish K and Varghese T, 2006. Probiotics In humans - evidence based review Celled MedlcalJoumal 2006, 4 (4).
• Krasaekoopt W.*, Pianjareoniap R.. and Kittlsuriyanont K., 2007. ProbioGcs rrult Juices. The 2 ^ International Conference on Fermentation Technology tor Value added Agriculbjrai Products. 2007, t(bonl<iaen,Jt\ai\anii.
Karasaekoopt W., Kitsawad K , 2010. Sensory characteristrcs and Consumer Acceptance of fiuit julca containing probiolics beads in Thailand. >IU J. T. 14f1): 33-38.
M»tln R Larga S , Reviriego C, ,Bmenez E. Main L M , CMvares M.. Boza J., ,&:ierier J., Fernandez L. -Xaus J . and RodnguK J.M., 2004, The"
commensal mcralioraofhumanmilk. NawperspectlvesftM-foodbacteriotherapyaridprobiotics 7mrKfen/=iMt/Scfe™:e*7ec/)nofog)'15.121-127.
McNaught C E. and MacFle J., 2001. Probiotics In clinical practice: a critical review of the evidence. Nutii. Res.2V. 343-353.
Monika T.. Danuta K.K. 2005. fijx attempt at using Lactobacilus acidophilus tor producing fermented carrot juice.
Yoon K.Y..WoodamsEe., and Hang Y.D., 2004. Probiolicsatron of tomato juice by lactc acid bactena. J. MfcmWo/. 42(4): 315-318.
Yoon K.Y. Woodams E E., and Hang Y.D., 2005 Production of probiotics cabbage juice by lactic acid bacteria.
UTILIZATION OF PROBIOTICS FOR PRODUCTION OF FERMENTED CARROT JUfCE
N g u y e n Thi Hanh'", N g u y e n Thi Thanh T h u y \ Hoang T h i Le H a n g ^ Nguyen Thi Dieu Thuy*
Hand University of Agriculture Fnjlt and VegetaWe Research Institute SUMMARY
Fruit mice containing probiotics has recently attracted inceiest of consumers. Tins study was earned out to assess the suitability of carrot juice aa raw material for the production of probiolics juice with three strama of LaetobaeHlus addopktlus HT, Lactobacillus fermintum HAG aod Bacillus elausii.
The bacteria have been sdded Io cairot juice with concentration 10* CFU/mL The duuige inamount of live bacterial cells, sensory quality of carrot
• 'ce during storage at 4'C have been analyied. The results indicated Ihu Lat^obaanus acidophilus HT got belter survival ability in carrot juice than two remained strains. Lsctobacillus acido[Jiilui HT strains have coottnued bdnensed to detennine the iofluence of bacteria ratio (1,3,5,7%) (v/v) oo the feraientaoon ability. Hie resulB showed that camrt Juice femieoted hy lactobadllus aadr^lalus HT with 3% and 5% coocentration at 37°C durina 24h has given Ihe high biomass and a good organoleptic quality for canot juice probiotics products. However, the sample with auppleraented -jcrobial biomass coneentiatiooa of 5% to make piarantee (he living cells b camK juice afler 40 days of storage at 4°C was more than lO'CFU/raL.
The prelimmary studies showed that canot juice has been suitable tor production of probiotics drinks.
•Author for con«spondence; Tel. 04 62617719; Email. [email protected]