TAPCIieiKTlNllS
NGHIEN CLfu THANH PHAN HOA HOC TINH DAU VO QUA CAM SANH (CITRUS
NOBILIS), CHUC (CITRUS HYSTRIX) VA CHANH TAY (CITRUS LIMON)
• PHAM TH! KIM P H U O N G - NGUYEN BA TRUNG - NGUYEN THj NGQC HAN - NGUYEN HLfU NGHI - LE TH! THANH XUAN
TOM TAT:
Bh'i viet lien hanh nghien ciJu cac ihanh phan h6a hoc cua tinh d^u v6 qu3 cam silnh (Ciirus nobilis) Lai Vung - Dong Thap. chiic (Ciirus hyslrix) Tinh Bien - An Giang vh chanh tay (Citrus Union) Chd Mdi - An Giang, bSng phUdng phiip chiTng ca't loi cuon theo hdi nifdc v^ lam khan bdi Na-jSOj. Sau do, SIJ" dung phu'dng phap sac ky khi gh6p khoi phd GC- MS, xac dinh difdc 43 cS'u txt trong tinh dau v6 qua cam sanh, thanh phan hoa hpc chinh gom thymol, o-cymene, caryophyllene, irans-a-bergamotene. caryophyllene oxide; 26 hdp cha't ti-ong tinh dau v6 qua chiic vdi thanh phan h6a hpc chinh D-limonene. p-pinene. sabinene, terpinen-4-ol, cyclene, 3-carene va 32 thanh phan h6a hoc trong tinh dau vo qua chanh tay vdi cac ca'u tir chinh a-pinene, P-pinene, D-limonene, y -lerpinene. geraniol, citral. neryl acetate.
Kel qua cho tha'y, cac hdp chat a-Pinene, P-Pinene, D-limonene, linalool, terpinen-4-ol, p-Ocimene. caryophyllene, p-Bisabolene deu c6 mat trong ca 3 Ioai tien vdi ham iifdng khde nhau.
Tiif khoa: Cam sanh Lai Vung, chue Tinh Bien, chanh tay Chd Mdi, th^nh phan hoa hpc, tinh An Giang, tmh Dong Thdp.
LDat va'nde
Chi cam qu<l {Citrus) cd khoang hdn 20 loai, phan bo tif nhien cf An Do. Trung Quoc. Viet Nam, Lao, Campuehia. Thai Lan va Mianma. Trong do.
taing tam phong phu \a da dang nhat la khu vi/c An D(> va Malai\ia( 15]. 6 VieiNam. chi Citrus-bao gom cac loai nhif cam. qu> i. chanh. bifdi... la nhu'ng ca>' in qua co gia tri kinh te cao. du'de trong pho bien lu- lau iren cic viing dja 1>' khac nhau [7 - 8].
Tmh (jau \ 6 qua. hoa \a la cua nhieu giong nay co hoai tinh sinh hpe. nhif. chong n\\ hoa. khang Mcm. khanii khuan. LTC che cac tO' bao u'ng thif va ngan ngifa cac benh ve mn mach | 1 ! . 13, 22).
N^oai ni. phan ldn iinh dau d Lhi cam chanh la
nguon nguyen lieu quan trpng trong cong ngh^ che bien thi/c pham, dUdc pham va hu'dng lieu |7, 18].
Tinh dau cua cac Ioai cam chanh difdc nghi6n clJu va cong bo thanh phan hda hpc du'di hinh thu'c h&
thong cac nhom chat terpene, alcol, sesquiterpene chu yeu tren la mgl so loai. Tuy nhien, viec he thong cac nhom chat tren vd quii cua chi citrus vin conhanche'[14.231.
^ Bai Met nghien cu'u vdi muc ueu cung cap mot so dan lieu co he thong then nhom ch^t cua tmh dSu tach tif vd qua 3 gi... ,.,„ .^^h (Citrus nobilis) Lai Vung - D6n . .p. chiic fCilrus hyslrix) Tinh Bien - An Gk
limon) Chd Mdi - .An Giani! -iuinh tay fC/rrw.y
358 So 8 -Thongs. 2020
IDA IQC-CDNG NGflEmife PIAM
1 Dtfl Itf^g ^^ phtfrfng phap nghi§n ciJu llBditmi'^ShiencvCu
Doi nJ^ng nghien CU^J lil vb qua cam sanh, chue
>ichanh iSy Addc thu hai tai cac huyen Lai Vung - EJong TMp. Tinh Bien - An Giang va Chd Mdi - Ao Giang.
21 PhUmgphdp nghien cOu
Tmh dau v6 qua cdc giong cam chanh difdc trich lv ling phu'dng phap chifng cat loi cuon hdi nu'dc ieo lieu chuan Difdc dien Viet Nam III [3]. Chgn nhihg qii3 tifdi vij'a chin tdi, vd nhan, lu'a de rao nu8c. bd cuong, lay phan vd qua xay nhuyin va diiflig cfi't loi cuon hdi nifdc. Phan dich sau khi dHfng c^l chia ih&nh hai ldp, tien hanh chie't nhieu lao tich lay phan dau d ldp tren va lam khan bdng NsiSOj. ihu difdc tinh dau san pham. Tinh dau Citnis nobilis thdm nggt diu thong mQi, mau trang pliptahh nhat. Citrus hyslrix thdm n6ng va Citrus tipicd mdi chanh pha lEn miJi sa, ca hai tinh dau dh c6 mSu vang nhat, nhe hdn nu'dc. Ham lifdng lmh dau v6 qua cdc gio'ng cam sanh, chiic, chanh iSyfJugc linh theo nguydn lieu tifdi.
Xkdinhty trpng b^ngty trpng ke'thuy tinh. D6 bda tan ciia tinh dau trong ethanol va cdc chi so' acid (IA). ester (IE) cua tinh dau cam chanh du'de xdc ij^theo tigu chuan Phap [2].
Thiinh phdn hda hgc cua tinh d^u difdc xdc dinh bSng phiftJng phdp siic ky klii (GC) va sac ky khi ghfip kh^i pho (GC/MS), tai trung tam phan tich Tn(9ng Dai hoc Khoa hgc tif nhien TP. Ho Chi Minh, S^c k^ khi 6890 N tren mdy Agilent (USA):
bao g6m bO tiem tif dpng, buong tiem, 16 cot, dau d6 MS 5972. Dac diem cot HP5 MS (30m x 0.:5mm X 0.25pm}. Nhiet do buong tiem 280°C vdi die do tiem split; ii le chia dong 50:1; nhiet dp dau
^ 150^. Chifdng trinh nhiet thifc hidn nhif sau:
nhiei d6 cpt 50 - 300''C, t6'c dp tang nhiel 0 - l(l°C/phdt, thdi gian giir nhiet 5 phiit. Toe dp khi nung 1.0 ml/phut, &d dung khi He vdi the ion 1900 EMV. Cdc thanh phan tinh dau du'de xdc nhan bang c^ch so sdnh cdc dd kien pho MS vdi pho chuan da 4*?Cc6ngbo trong thu-vien NIST 14.
S-Kg"! qua va thao lu§n
Ham lifdng tinh dau vd qua cam sanh Lai Vung. chdc Tinh Bien va chanh tay Chd Mdi so
^nguyen lieu ttfdi tu'dng ijng la: 0.327r: 0,25%
u(),27^f.
Tir sci'lieu Bang 1 cho tha'y, ty trgng ciia vd qua
= ^ chdc va chanh tay Ian lifdt la 0.932: 0,920:
' ^'>'^ deu nho hdn 1, nen nhe hdn nifdc va noi tren
Bang 1. Chi so hoa ly cuo Hnh dau Kf vo qud c a m , chue vd chanh toy Chi so
ly-hda Cam sanh 1 Chue
Chanh tay
ry
Irong 0,932 0,920 0,868
Chi so Add
(IA) 1,121 4.208 1.581
Chi so Estgr (IE) 0,432 21,038 0,882
B^hoalan trong ethanol
90%
14.01 14,56 1,531 be mat nu'dc. Chi so acid (IA) va ester (IE) cua cam.
chanh tay lan Ii/dt 1.121; 1.581 va 0.432; 0.882.
Trong khi. chue cd chi so' tu'dng u'ng kha cao - 4.20S va 21.038 - nen kha nang chu'a nhieu acid beo hdn.
Do hoa tan trong ethanol 90% cua cam. chue. chanh theo trinh tif 1:4.01; 1:4.56: 1:531 cho tha'y. cdc tinh daudeu tan to't trong cac dung mdi phan cifc.
Bdng 2. He thong phan loqi cdc nhom chdt chifa thdnh phdn hoa hoc
com, chue. chanh tdy
Tenhdpdtat
Ham liJdng% tinh dSu trong v6quS Cam sanh Monoterpene
Sesquiterpene Diterpene Monoterpene aldehydes Monoterpene alcohols Monoterpene esters Monoterpene phenol Sesquiterpene alcohols Sesquiterpene oxid Sesquiterpene esters
"long ldp chatterpenhydro long dan xuat chiia oxy CLia terpen
15,96 7.08 0.46 2,09 5,33 0,13 55.11
9,182 0,24 24,14
75.85
Chanh tay 77 2,93
8,71 6,77 3,22 0,08 0,1
30.52
Ket qua tai Bans 2 cho thay. linh dau vo qua cua cdc "ion" Cam chanh la hon hop gom nhieu ihanh phan khac nhau cua hydrocarbon, alcohol.
So 8 - Thang 4/2020 359
TAPcijctiitmnK
aldehyde, ketone \a ester. Trong do. thanh phan thupc ldp chat lerpenhydro chiem khodng 24.14 - 79.93 % \ a thanh phan thupc ldp dan xua't L-hi/aovv cOa terpen chie'm 19.76-7S.S,> ^ .
3.1. Tinh tidu vd qud cam sdnh Lai Vung Tinh dau cam sanh cd miu thdm ngot dju. thu' thai, thong mui. vi hdi ca>. mau trang pha xanh nhat.
Hinti 1: Sac kidS tinh ddu vo qua cam santi Lal Vung
Dif iieu phd do cu.i hda hpc ciia tinh dau \i 43 hdpcha't, baogdiii lerpenh>dro - chiciii 2 15.y6'r: sesquiierpeiie
.:iaj 1.1 ^iltriC.TO;
ULjqfiHmh 1 cho ili.i). Ihiinh phan ,, uii,:..:iii >:inhLai Vungcd
-h.iii ihupcldpchd't 4 1 !•, (nhdm monoterpene:
0 8 ' ' . diterpene: 0,46%
va thanh phin khac: O.M'^n va 23 thanh phan thupc ddn xuat chu'a o\y ciia terpen - chi6m 7.^.85^ (nhdm monoterpene alcohols: 5,33%;
monoterpene aldehydes; ' 2,09; monoterpene esters:
O.I3'r: monoterpene phenol:
>(•>.[ I ' r . sesquiteq)ene ;' esters; 0.24'7f. Sesquiteipene ^ oxid: 9,182% va thiinh phan :
khac: 2,77%). ' 3.2. Tinh ddu vd qud chiic ;'
Tfnh Bien ^ Tinh dau vd qua chue Tjnh f
Bien la chat long trong sudt, mau hdi vang, cd vi cay, lhe '•
va mui thdm n6ng ddc trifng, t tao cam giac thoai mdi.
Bdng phifdng phdp GC- MS xdc dinh difdc 26 thdnh phdn hda hpc cua tinh dau vd ' qua chue Tinh Bien, trong dd !i cd 15 thanh phan thupc ldp i' chd't terpenhydro, chie'm 1 69,2% {nhdm monoterpene: i>
68,06%; thanh phan khde: •:
1,14%) va 11 thanh phan i thupc dan xud't chu'a oxy ctia li terpen chiem 30,52% (nhdm ti monoterpene alcohols: t 29,43%; thanh phan khde: !•
1,09%).
tei:
Hinti 2: Sac ki dS tint) ddu vo qua chue Tint) Bien
360 So 8 - Thang 4/2020
BOA IOC-CONB NGIIETIItfC PIAM
j j : Tuih ddu vo qua chanh Hinti 3: S6c ki do tinti dau vo qua ct)anfi tay Clip Mdi
Tinh diu v6 qud chanh tay la ckl'i loag trong suot, mau v a n g
^ l , vi cay. the, m&i chanh pha
!{• mui si, tao cam gidc nhe
^dDg sang khoai, nhe hdn oik.
Tif s^c ki do cua Hinh 3, xdc dmh diTtfc 32 thdnh phan hoa hpc Dcn tinh dau vo qud chanh tay a^Mdi vdi 14 thdnh phan thupc
|(}p chS'i terpenhydro - chiS'm 79.93% (nhdm monoterpene:
77%; sesquiterpene: 2,93%) va 18 thinh phan thupc dan xuat difb oxy ciia terpen - chie'm 19.76% (nhdm monoterpene alcohols: 6,77%; monoterpene aldehydes: 8,71%; monoterpene eaere: 3.22%: monoterpene phenol: O.OS^o; sesquiterpene alcohols: 0,1 % vd thanh phan iMc:0,88%).
}.4.Tbaoludn
Do kha ndng u'ng dung cao Bong nhieu lmh vtfc - nhif thifc
pham, y hoc. htfdng lieu - n e n da cd n h i e u cong trinh nghien cd^i vdi muc dich xdc dinh thanh p h a n cila linh diu vd qua thude chi C a m chanh [5, 17.
26J. Tinh dau cam chanh chu'a Iifdng ldn nmoteipene va sesquiterpene, thanh p h a n chu'a oiy dSn xuat di cdc hydrocarbon, g o m : alcohol, lidehyde, ketone, ester, ether, phenol va oxide.
MngthSi, thjinh phan chinh cua tinh dau vo qua
^icloai C nobilis, C. hyslrix, C. Iiinon trong cting
|WI chi c6 khac nhau, hau het d e u chO'a D^
imonene. p-myrcene, pinene, s a b i n e n e , 4cipinenevap-cymene[l,9,16].
Kei qud phdn tich cua nhdm tac gia v e tinh dau tif
* qui cua 3 giong Cam chanh C. nobilis, C. Iiysiri.x.
Jimoti cho thay, cdc hdp chd't nhdm m o n o t e r p e n e niem chu y^u (15,96 - 77%), thanh phan cd h a m J^ngldn nhat la p- pinene, sabinene, D- limonene
" ^ y " i e " e tuwng dong vdi kd't qua nghien cu'u W. Ddng chu y Id cdc thdnh phan nhu" thymol.
"yopfiyllene oxide, citral, geraniol acrylate cd h a m pug khi cao trong nhdm d i n xua't chu'a oxy cua [Pene (chie'm 19,76 - 75,85%). Day la dieu khde e' kha ro khi so sdnh vdi tinh dau nhieu loai c a m
quyt vdi cdc cong trinh da cong bo de cap d tren.
Ttjf ke't qua phan tich cho tha'y. tinh dau vd qua cam sanh bao gom cdc thanh phan chinh nhu': thymol (54.48'>i-), o-cymene (9.77%), caryophyllene (2.09%), mins-a-bergamotene (3,16%).
caryophyllene oxide (9,18%). Dac biet, cac hdp cha't D-limonene. y-terpinene. linalool, p-menth-2.8- dien- l-ol, terpmen-4-ol, cii ng nhu' trans-p-mentha-l{7)-8-dien-2-ol. biosol, limona ketone, cd ham lifdng < 2%. Nhan tha'y, cdc thanh phan limonene. y-terpinene. linalool, a-pinene. beta pinene. a-thujene. myrcene. terpinen-4-ol trong nghien cdu cua chung toi phu hdp vdi ddn lieu phan tich cua Nevcihan Gursoy va Qingyun Guo. Mac du CO khde nhau ve ham lu'dng [20, 24] Theo cong bo Sreepian. A. Tnfdng Dai hpc Rangsi! (Thdi Lan).
Citrus hvstriv D-limonene (25.28'7f): p-pinene (21.10%); sabinene (14,99%); terpinen-4-ol (5.06%); 22 hdp cha't con lai cd ham lifdng t^ 0.11 den 2.82% [27]. Dieu nay phii hdp vdi ke't qua nehien cifu ve unh dau vd qua chiic Tinh Bien. gom cd 26 hdp chat- thanh phan chinh thupc nhdm monoterpene (68,06%)bao gom p-pinene(3(),52'r):
So8-Thdng4/2020 361
TAfCIJCdNGTIirm
D-limonene OS-35'f>; sabinen (30.96%); 3-Carene i2.42'r);c>clene i2.23''f) \a 21 hdp cha't con lai co ham lu'dng tif 0.03 den 0.91 %.
Kcl qua cua nhiing nhom nghien cihi tn/dc day cdng bd'. tinh dau chanh tdy chu'a cdc thanh phdn hoa hpc ^ u : a-lhujene: a-pinene: p-pinene; D- limonene: y-terpinene; p-menth-2.8-dien-I-ol;
terpinen-4~ol: p-bi(;abolene; a-terpineol: geraniol;
citral; nen. I jcciale: citronellal: camphene; asarone:
cjr>ophyllene; linalool; p-ocimene; {-t-i^-ciu-ene; a- car\(iphyllene; decanal: elemene; a-bergamotene:
a-bisabolol; germacrene D: cyclomethicone 7;
bomeol; car^aciol; eucalypiol va mot sd cdu tiJf khac [10, 19. 28) Sau khi so sanh. nhan thay ket qua nghien cthj cua nhdm tac gia gom cac thanh phdn hda hpc chinh la; a-thujene (0.58 %): a-pinene (2.30%);
p-pincne ll.-i.48%): D4imonene (4S.()6'"r). y- terpinene (9.72M: p-menUi-2.8-dien-l-ol (0.10%);
lerpinen-4-ol (1,09%); p-bisabolene (1,12%); a- lerpineol (1.73%); geraniol (2.73%): citral (8.53%);
ncr>l acetate (3.14%); ci[roncl!al(0.17%); camphene (0,11%). asarone (0.29%); caryophyllene (0,68%);
linalool (0.86%). p-ocimene (0.12%); (+)-4-carene (0,63%); a-caryophyllene (0,15%); decanal (0.21%);
elemene (0.25%); a-bergamotene (0.66%); a- bisabolol (0.10%'); nhutig thdnh phan cd ham lifdng <
0. i gom cd germacrene D (0.07%); cyclomethicone 7 (0,07%); bomeol (0,09%); carvacrol (0,08%);
eucalyptol (0.08%). Hau he't, cac thanh phan hda hpc trong ke't qua nghien cihi cua nhdm tac gia deu phu hdp vdi nhCftig nghien cdu da cong bo' tru'dc dd. Chi khac biet mot so' thanh phan vdi ham lu'dng rat nho nhif: p-menth-2,8-dien- l-ol (0.10%). citronellal (0.17%); camphene (0,11%); asarone (0.29%); p- ocimene (0.12%); (+)-4-carene (0.63%); elemene (0.25%); germacrene D (0.07%); cyclomethicone 7 (0.07'";):car\acrnl(0.08''n.
Tif ke't qua phdn dch. nhdm tde gia nhan tha'y 3 loai cam sanh. chiic, chanh ta\ cd thanh phan hda hoc tifdng u'ng vdi nhu'ng ke't qua nghien cu'u trifdc day, iu\ nhien cd sif khac nhau ve ham Iifdng. Dieu n:i\ CO the chihig minh vdi dieu kien dia ly klidc nhau. thanh phan va ham lifdng cdc chat trong tinh dau cua cung mdi loai cd the khac nhau [25]. Cdc hdp chat -pinene. p-pmene. D-limonene. linalool, terpinen-4-ol, p-ocimene. carvophyllene. p- bisabolene deu cd mdt trong ca 3 loai ca\ nghien cii'u. C-^c hdp cha'l la dan xua't o\\, citral igeranial
\ i neralKciu-onellal. decanal, ovianal Jong \ai trd qu\ei dinh den chji lu'dng miii ihdm cuns nhif gid
in thifdng pham cua Unh ddu cam ^:'-" '^J^^ ° ^ ,, ra. D-L.monene difdc .si^ dung -'••? !?hiep l^m >
nguyen Ueu cho chd't kei dinh. Imdng Ueu vd chat I nJa [4]. Tinh ddu cam sanh. chue va chanh tay (,t cuns xac dinh difdc Uia nang Khang oxy hda, „ khan, khud'n [12. 21. 29]. Day la nhiftig loa. cung , cap n.uven lieu chinh san xudt tinh dau cam quyt ^ tren ihc"ddi. didu ndy cho tha'y. tinh dau tach tif vo , qua 3 gid'ng cam chanh ndi tren deu cd gia h"! ve ^
challifdiii; , 4. Kel luSn , '
Tinh dau vd qua cdc gio'ng cam chanh gom 82 ^ hdp cha't. ffong dd tinh d^u cam sanh cd miii thdm iHi ngpt dju. thif thdi, thdng miii, vi hdi cay, mau h-ang :i pha xanh nhat, chu'a 43 ca'u tu" phan ldn thuoc nhdm ?•
monoterpene (15,96%); monoterpene phenol (56,11 %) vdi cdc thanh phan chinh nhu': thymol, o- , cymene, caryophyUene, trans-a-bergamotene, caryophyllene oxide.
Tinh dau chue la chd't long trong suot, mau hdi [ vang, cd vi cay, the va mui thdm nong dac trifng, '' tao cam giac thoai mdi, cd 26 hdp cha't chu y6'u ;, trong nhom monoterpene (68,06%); nhdm monoterpene alcohols (29,43%) bao g6m cac thanh '-•
phdn chinh: D-limonene, p-pinene, sabinene, '
terpinen-4-ol, Cyclene, 3-carene. s Vdi 32 ca'u t^ difdc xac dinh trong tinh diu chanh I
tdy cd cha'l long trong suot, mau vang nhat, vi cay, i:
the va mui chanh pha lan mtii sd, tao cdm gidc nhe ^ nhang sang khoai, nhe hdn nifdc. Trong dd, nhdm i monoterpene chiem 77%; nhdm monoterpene t, alcohols CO 6,77% va nhdm monoterpene i aldehydes khoang 8,71 %; phan Idn cac hdp cha't F chinh chu'a a-pinene, p-pinene, D-limonene, y- terpinene, geraniol, citral, neryl acetate,
Sau khi dd'i chie'u, nhdm tac gia nhdn tha'y cdc hdp cha't a-pinene, p-Pinene, D-Iimonene, linalool, ' terpinen-4-ol, P-ocimene, caryophyllene, p- "' bi.sabolene deu cd mat trong ca 3 loai vd qua citrus , vdi cdc ham Iifdng khac nhau. Cac chi so' ve tJ trpng cua cam sdnh, chue. chanh tay lan li/dt la 0,932; 0,920; 0,868 deu nho hdn 1, nen nhe hdn • nu'dc va noi tren be mat nu'dc. Chi so acid (IA) va i esler(IE)cijacam. chanh tay !dn lifdt 1,121; 1,581 va 0.432: 0,882. Trong khi. cliuc cd chi s6' tifdng • ifng khd cao - 4,208 va 21 .Q3H - nen kha nang chiTa i nhieu acid beo hdn. Dd hda tan irong ethanol 90%
cua cam, chue. chanh vdi li If; iheo irinh tU 1'4 01;
1:4,56; 1:531 cho tha'y. cac unh dau d^u tan to't irong cac dung moi phdn cut >
362 So B - Thang 4/2020
ISA IOC CONG NGHE M c NAM
t i l U l U T H A M KHAO:
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3 i M So 8 - Thang 4/2020
lOA IOC-CONG NGHE Tide PIAM
A STUDY ON THE CHEMICAL COMPOUNDS
OF THE ESSENTIAL OD^S EXTRACTED FROM CITRUS NOBILIS, CITRUS HYSTRIX AND CITRUS LIMON
• PHAM THI KIM PHUONG
Lecturer, Faculty of Pedology, An Giang University Vietnam National University - Ho Chi Minh City
• NGUYEN BA TRUNG
Lecturer. Faculty of Agriculture - Natural Resources, An Giang University VIETNAM NATIONAL UNIVERSITY - HO CHI MINH CITY , " N G U Y E N THI NGOC HAN
Stutdent, DH 15HH, Faculty of Pedology, An Giang University Vietnam Notional University - Ho Chi Minh City
• NGUYEN HUU NGHI
Student, DHl 7HH, Faculty of Pedology, An Giong University Vietnam National University - Ho Chi Minh City
• LE THI THANH XUAN Dong Thap University
ABSTRACT;
This study is to identify chemical compounds of the essential oils exffacted from Lai Vung Cilnts nobilis {Dong Thap Province), Tinh Bien Ciirus hyslrix (An Giang Province) and Cho Moi Citrus limon (An Giang Province) by using the steam disttillation method with the anhydrous sodium sulphate (Na2S04). By using the gas chromatography-mass specffometry (GC-MS) method, fliis study finds that the essential oil of Lai Vung Citrus nobilis has 43 chemical compounds including major chemical compounds of thymol, o-cymene. caryophyllene, trans-a- bergamotene and caiyophyllene oxide; there are 26 chemical compounds in the essential oil of Tinh Bien Ciirus hyslrix including major chemical compounds like D-limonene, P-pinene, sabinene, terpinen-4-oI, cyclene and 3-carene and there are 32 chemical compounds in the essential oil of Cho Moi Ciirus limon including a-pinene, Ppinene. D-limonene, y -terpinene, geraniol, cih'al and neryl acetate. All these three essential oils have chemical compounds including a-Pinene, P-Pinene, D-limonene. linalool, terpinen-4-ol, p-Ocimene. caryophyllene, p- Bisabolene with different levels.
Keywords: Lai Vung Citrus nobilis, Tinh Bien Citrus hyslrix, Cho Mot Ciirus limon, chemical components. An Giang Province, Dong Thap Province.
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