Nghien
CLFU- Ky thuat
Tal lieu tham khao
1. D 3 Huy Bich, Ding Quang Chung, Nguyin Thupng Dong v i cs. Cay thuoc v i dpng vat lim thuoc d Viet Nam, tap 2, NXB Khoa hgc va ky thudt. Ha Ngi, (2004) tr. 214-215.
2. Do Tat Lpi, Nhung ciy thuoc va vj thude Vipt
Nam (Xuit ban lin thir X), WXB Yhgc, Hd we/.(2003).
3. Hudng dan kim theo Quyet djnh so 371/BYT - QD ngay 12/3/1996 cua Bp Y t i ve xac djnh tinh an toan cua thude y hpc co truyen.
4. General guiderlines for methodologies on research and evaluation of traditional medicine. World Health Organization 2000.
Birfrc dau danh gia tac dung ha glucose huyet ciia re cay choc mau (Salacia cochinchinensis)
tren chuot nhat bi tang glucose huyet bang streptozocin
Do Thi Nguyft Que ', Nguyen Tran Thj Giang Huomg^
Nguyen Duy Thuan', Nguyen Viet Than' Nguyin Thi Kim Hue '
' Tne&ng dgi hgc Dugc Hd Ngi
^Truang dgi hgc Y Hd Ngi
^ Vien Duac lieu Dat van de
D i i thio dudng l i benh ed tde dd phat trien nhanh, dupe xem l i "dai djch" d c i c nude dang phit trien. Theo WHO, nam 2025, se ed 300 - 330 triiu ngudi mie benh, chiem ty l i 5,4% din sd toin ciu, trong dd chu y i u l i BTO type 2 (85- 95%) •''.
Viet Nam l i nude dang phit trien, benh DTD eQng dang ting lin nhanh ehdng theo thdi gian.
Dieu tra qude gia nim 2002 - 2003, ty le mie benh trong ca nude l i 2,7%, chu yeu t i p trung d lira tudi lao ddng 30 - 64 tudi (chiem ty l i 10,5%) v i chiim ty l i cao d vimg dd thi v i khu cdng nghiip (4,4%), die biit l i d c i c thinh phd Idn.
Hiin nay d i cd nhieu thude t i n dupe dupe su dung de diiu tri binh d i i thio dudng v i cho hiiu qua diiu tri tdt tuy nhiin hiu hit c i c thude niy ed nhiiu tie dung khdng mong mudn, die biit khi sir dung thdi gian d i i . Bin canh eie thude tin dupe, hien cung ed nhieu thude ddng dupe dupe nhin d i n sir dung de dieu tri d i i thio dudng '^'. Tie dung eua eie thude niy tuy khdng nhanh v i manh bing thude t i n dupe nhung thudng cd it tie dung khdng mong mudn, it dde tinh v i phii hpp vdi y i u cau dieu tri liu d i i ciia binh. Do dd, xu hudng nghiin ciru de tim ra 28
nhirng dupe lieu cd t i c dung ha dudng huyit vS diiu trj d i i thio dudng dang thu hut dupe nhieu su quan t i m cua eae n h i nghiin ciru.
Theo kinh nghiem d i n gian mdt sd nude, nhieu ciy trong loii Salacia nhu S. oblongga, S.
retlculat, S. chinesis, tir l i u da dupe dung de diiu trj bio phi v i d i i thio dudng cung nhu eic binh liu, khdp, hen....'^' Tai Nhit Ban, My vS mdt sd nude khic r i Salacia dugc diing dudi dang thuc pham chirc nang dinh cho ngudi tieu dudng hoic b i o phi. Nhieu nghiin ciru cung dS dupe tien hinh de x i c djn-h thinh phin hoi hpc, tie dung cua mdt sd c i y trong loii Salacia trong dd cd dinh g i i t i c dung ha glucose cua S.
chinesis '^•^•^' . Tuy nhiin, chua cd cdng trinh nghiin ciru nio ve t i c dtjng chua tieu dudng vA bio phi eua c i y chdc m i u - Salacia cochinchinensis - l i loii tim thiy d Viet Nam '".
Nham tim hieu tie dung g i y ha glucose huyet cua ciy chdc miu, chijng tdi tien hinh dinh giS tie dung giy ha glucose huyet ciJa loii niy trSn chupt nhit bj ting glucosse huyet bing streptozocin.
Nguyen lieu va phij'O'ng phap Nguyen lieu
R i c i y Salacia cochinchinensis Lour, dupe
Nghien
CLFU- Ky thuat
Viin Dupe liiu (TS. Nguyin Duy Thuin, CN Ngd Vin Trai) cung cap, dupe khoa Tii nguyin - Viin Dupe liiu xic djnh tin khoa hpc v i luu.
Sau khi thu hii, re ciy dupe dem nJa sach, siy khd d 70°C, tin nhd.
Chuin bj miu nghien ciru:
- Dupe lieu da lim nhd dupe chiet bing methanol, eat thu hdi dung mdi d i p suit giam, hda ein vio nude thu dupe cao toin phin 1:1 (tinh theo dupe lieu).
- Hda cin djch chiit toin phin vio nude, dem lie vdi eic dung mdi cd dp phin cue ting din: n-hexan, elorofomn (CHCI3), ethyl acetat (EtOAc), butanol (BuOH). c i n eua eie phin doan trin dupe pha trong dung djch HPMC 3% tarde khi cho chudt udng.
Ddi tuang
Chudt nhit tring chung Swiss ca hai gidng, khde manh, trpng lupng trung binh 18 - 22g, do Viin V i sinh Djch t i Trung uang cung cip. Chupt dupe nudi 3-5 ngiy trude khi lim thi nghiem tai phdng thi nghiim bd mdn Dupe ly trudng Dai hpc Dupe H i Ndi. Chupt dupe cho an thirc an chuin do Viin V i sinh Djch te Trung uang cung cip v i udng nude tu do.
Phuang phap nghien cu>u
Phuang phdp ddnh gid tdc dung ha glucose huyit trdn chupt gdy tdng glucose huyit bdng streptozocin (STZ)
Chupt nhit tring sau khi dugc nudi dn dinh glucose huyit trong diiu kien phdng thi nghiim 3 - 5 ngiy, bj ting glucosse huyet bing cich tiim ming bung STZ 1 liiu duy nhit 150mg/kg pha trong dung djch dim citrat (pH: 4,5-5). Djnh lupng glucose huyet sau 72h, lua ehpn nhCrng con cd glucose huyet ting cao >10 mmol/l v i chia thinh eie Id.
Cho chupt udng thude, danh gii tic dung ha glucose huyet ciia cie Id nghien eiru bing phuang phip so sinh gii tri glucose huyet tai thdi diem torde v i sau khi udng thude. Song
Bang 1: Tdc dung tren glucose
song tien hinh Id chirng (udng dung djch pha thude) de so sinh.
Dudng huyit ciia chudt thi nghiim dupe do bing miy do dudng huyit Johnson & Johnson.
Miu d i dinh lupng dupe liy tir dudi chudt (bd di gipt miu diu tiin).
Sd lieu dupe xir ly thdng k i theo phin mim Microsoft Excel 2003. Su khic nhau ed y nghTa thdng ke khi P< 0,05. Kit qua thi nghiem dupe bieu thi bang trj sd trung binh epng/trir sai sd chuin (M ± SE).
Kit qua va banluan
Anh hudng cua djch chiit toan phin r l cay chdc mau tren glucose huyit cua chupt gay tang glucose huyit bing streptozocin (STZ).
Chupt nhit tring sau khi nudi dn djnh trong dieu kiin phdng thi nghiim chpn ngiu nhiin 7 con lim Id chirng tring
Ld 1 (n=7): chirng tring, eho udng dung mdi pha thude
Sd chupt cdn lai tiim ming bung STZ d i giy ting glucose huyet. 72 gid sau khi tiim STZ, djnh lupng glucose huyit v i ehpn nhirng con cd dudng huyit >10 mmol/l chia thinh eie Id:
- Ld 2 (n=8): Chirng STZ, eho udng dung mdi pha thude
- Ld 3 (n=9): Cho udng cin dich ehiit toin phin vdi lieu 0,69g/kg (tuang duang vdi 2,88 g/kg chupt l i liiu tuang duang vdi lieu diing cho ngudi)
- Ld 4 (n=8): Cho udng ein djch chiet toin phin vdi lieu 3,50g/kg (tuang duang vdi 14,4 g/kg chudt)
- Ld 5 (n=9): Cho udng gliclazid vdi lieu tuang duang 19,2 mg gliclazid/kg chudt
Cic thude thir deu dupe pha de dam bao the tich thude (hoic dung mdi pha thude) cho udng 1 lan l i : 0,1 ml/10g chudt Tiin hinh djnh lupng lai glucose huyet sau khi udng thude hoac dung mdi pha thude 4 gid.
huyit ciia djch chiit todn phin
Nong 6Q (Ju'ang huyet trung binh (mmol/l) j y |0 % (,a Oh 4h glucose huyet Chu'ng trang
(n = 7) Chu'ng STZ
(n = 8) L6 uong thuoc lieu 0,69g/kg
(n = 9) Lo uong thu6c li^u 3,50g/l<g
(n = 8) Gliclazid ( n - 9 )
8,23 ± 0,53 12,34 ±0,58 14,43 ±2,04 12,49 ±1,32 16,18 ±1,66
7,40 ± 0,80 P = 0,26*
11,33 ±0,79 P = 0,09*
9,19 ±1,40 P = 0,003*
6,28 ±0,55 P = 0,00038*
10,00 ±0,99 P = 0,00026*
35,64 P, = 0,24
47,40 Pi = 0,88 P2 = 0,08 38,20
TAP CHI DUQC HQC - 7/2009 (SO 399 NAM 49) 29
Nghien ctpu - Ky thuat
(*); So vdi thdi diim Oh. P,, So vOi IP udng gliclazid. Pj. So glQ'a 2 IP uing thuic
Nhu viy, c i n djch chiet methanol r i c i y chdc m i u , d thdi diem 4h sau khi diing thude, vdi liiu 0,69g/kg v i 3,50g/kg, deu cd t i c dung ha glucose huyit so vdi thdi diem ehua udng thude (P<0,001). Ld chirng tring v i Id chirng STZ khdng ed t i c dung ha glucose huyet (P>0,05), trong khi dd Id udng gliclazid ed t i c dung r i t rd (P<0,001), dieu dd chirng td vdi lieu 150mg STZ/kg chupt duy nhat tiim ming bung chua p h i huy hoin toin dao tuy v i chupt v i n d i p irng tdt vdi thuoc d i i u trj DTD dudng udng (gliclazid). Ty l i % ha glucose huyit d 2 Id udng thude v i Id udng gliclazid lan lupt l i : 35,64; 47,40 v i 46,12. Su khic biet khdng ed y nghTa thdng k i (P>0,05).
Tai Viit Nam v i tren t h i gidi hiin nay ehua ed nghiin eiru n i o v i t i e dung cua c i y Salacia cochinchinensis trin glucose huyit, mac du trin the gidi d i ed k h i nhieu nhirng nghien eiru ve t i e dung ha glucose huyit, chdng bio phi eua eic c i y khic thupc ehi Salacia, dieu niy md ra mdt hudng di mdi trong nghiin ciru gdp phin l i m phong phii thim ngudn dupe liiu cd t i e dung ha glucose huyet d Viit Nam.
Kit qua thu> tac dung cac phan doan cua djch chiit chdc mau tren chuot nhit tring bj tang glucose huyit bing STZ
Chupt nhit tring sau khi nudi dn djnh 3-5 ngiy trong phdng thi nghiim, chpn ngiu nhiin 9 con l i m Id chirng tring:
Ld 1 (n = 9): Id chung tring, udng dung mdi pha thude
Sd chudt edn lai tiim ming bung STZ lieu duy nhit 150mg/kg de giy t i n g dudng huyet, chpn nhirng con chupt cd glucose huyit s 10mmol/l chia thinh eie Id:
Ld 2 (n = 8); chirng STZ udng dung mdi pha thude (dung djch HPMC 3 %)
Ld 3 (n=13): udng DC/n-hexan vdi lieu 0,0019g/kg chudt*.
Ld 4 (n = 6): udng DC/CHCb vdi lieu 0,034g/kg chudt*.
Ld 5 (n =11): udng DC/EtOAc vdi lieu 0,0031 g/kg chupt*.
Ld 6 (n = 12): udng DC/BuOH vdi lieu 0,021 g/kg chudt*.
30
* Lieu dung n i y tuang duang vdi liiu tfnh ra dupe liiu khd l i 2,88g/kg chudt.
Tien hinh djnh lupng lai glucose huyet sau 4h cho udng thuoc hoic dung mdl pha thude, thu dupe k i t qua nhu sau:
Bang 2: Tdc dung cua cdc phdn doan dich chiit
Ld
N6ng (3d du'd'ng huygt trung binh (mmol/l)
Oh 4h
Ty le % ha glucose
huy§t Chu'ng
trjng (n = 9)
8,30 ± 0,31
7,93 ± 0 , 1 9 P = 0,39*
Chu'ng STZ (n = 8)
14,70 ± 1,20
17,64 ± 2 , 0 2 P = 0,07*
DC/n- hexan (n = 13)
15,50 ± 1,17
9,05 ± 0,73 P = 0,000001*
41,11 17.80 ±
2,71
(n = 6) P = 0,06*
DC/CHCIj 13.38 ± 1 . 4 8
DC/EtOAc 13,63 ± 9,82 ± 0,82 25,96 (n = 11) 0,87 p = 0,0051 * P = 0,035"
DC/BuOH 15,98 ± 16,52 ± 1 , 8 2 (n = 12) 1,41 P = 0,48*
(*): so vdi thdi diem Oh.
(**): so % ha glucose huyet cua Id udng cin djch ehiit trong n-hexan v i trong ethylacetat
Kit qua thye nghlpm eho thay, trin md hinh chudt bj ting glucose huyet bing STZ, sau 4h udng thude, c i n djch chiet trong n-hexan v i trong ethyl acetat cd t i c dung ha glucose huyit vdi ty l i % tuang iJng l i 41,41 v i 25,96. Phin doan n-hexan cd t i c dung manh ban phin doan ethylacetat (P<0,05). 2 phifi doan DC/CHCIsVdi liiu 0,034g/kg v i DC/BuOH vdi lieu 0,021 g/kg khdng cd tie dung niy.
Trin ea sd k i t qua tren chiing tdi tiep tuc tien hinh thi nghiim vdi lieu cao gap 5 lan lieu dOng trin v i dinh g i i kha ning anh hudng din glucose huyet d eic thdi diem 2 h, 4 h v i 6 h ciia 2 c i n djch chiet trong n-hexan v i trong ethyl acetat nhim x i c djnh lieu diJng v i thdi diim thich hpp chd c i c nghiin ctru tiep theo. Ket qua dupe trinh biy trong bang 3'
Nghien
CLPU- Ky thuat
Bing 3: Tdc dgng ciia phdn do?n djch chiit trong hexan vd trong ethyl acetat
Ld Chi>ng tr^ng Chi>ng STZ (n = 7) (n = 7)
DC/n-hexan (n = 8) (1) (2)
DC/EtOAc (n = 8) (1) (2) Oh
2h
7,74 ± 0,41 8,24 ± 0,25 P = 0,26*
8,40 ± 0,49 P = 0,21*
21,08 ±1,64 18,32 ±2,62 P = 0 , 2 1 ' 15,43 ±2,48
P = 0,060*
16,17±2,61 P = 0,092*
18,90 ±2,15 12,96 ±2,05 P = 0,0015*
12,12 ±2,96 P = 0,017*
13,1 ±2,60 P = 0,016*
32,09 37,56 P4.2= 0,64
31,22 P6.2 = 0,93 P M = 0,63
15,50 ± 1,58 12,76 ±1,59 P = 0,0033*
13,24± 1,37 P = 0,012*
11,78 ± 1,88 P = 0,024*
17,25 P= 0,042**
13,86 P= 0,047**
?«'= 0,73 24,02 P=0,53**
P6.2 = 0,39 Pa-4 = 0,27
(1); Ning dp glucose huyit trung binh (mmol/l)
(2); % ha glucose huyit (*); so vdi thdi diim Oh.
("): so sdnh 16 dung cdn djch chiit trong n- hexan vd trong ethyl acetat d cung thdi diim
P4.2; so sdnh % ha glucose huyit d thdi diim 4h so vdi 2h
Pfi-s.' so sdnh % ha glucose huyit d thdi diim 6h so vdi 2h
P6.4: so sdnh % ha glucose huyit d thdi diim 6/7 so v&i 4h
Kit qua thue nghiim cho thiy, 2 phin dogn djch chiit trong hexan vdi lieu 0,0094g/kg v i trong ethyl acetat vdi liiu 0,016g/kg diu cd tic dyng ha glucose huyit sau udng thude 2h, 4h, 6h so vdi thdi diim chua diing thude (vdi P<0,05). Ty l i % ha glucose huyet eiia Id djch chiet trong n-hexan lin lupt l i : 32,09; 37,56;
31,22. Ty l i % ha glucose huyit eua Id djch chiit trong ethyl acetat lin lupt l i : 17,25; 13,86;
24,02
& thdi diem 2h v i 4h sau udng thude, djch chiet trong n-hexan cd t i c dung manh ban dich chiet trong ethylacetat (P<0,05), tai thdi diem 6h su khie biit niy khdng cd y nghTa thdng k i (vdi P>0,05).
Vdi Id udng c i n djch chiet trong n-hexan, ty i i
% ha glucose huyit cao nhit sau '4h udng thude tuy nhiin % ha glucose huyit cao sau 2h, 4h v i 6h khie nhau niy khdng cd y nghTa thdng k i (P>0,05).
Vdi Id udng c i n djch ehiit trong ethyl acetat, ty l i % ha glucose huyet cao nhit sau 6h udng thude tuy nhiin % ha glucose huyet cao sau 2h, 4h v i 6h khic nhau niy khdng ed y nghTa thdng k i (P>0,05).
TAP CHi DUQC HQC - 7/2009 (SO 399 NAM 49)
Ket luan
Qua nghiin eiru thuc nghiem tren md hinh chupt giy ting glucose huyit bing STZ, ehiing tdi riit ra mdt sd kit luin sau:
1. Cin djch chiet toan phin r i ciy chdc miu trong methanol vdi lieu 0,69g/kg v i 3,50g/kg chupt, d thdi diem 4h sau khi diing thude, diu ed tie dung ha glucose huyet vdi % ha glucose huyit tuang irng l i 41,11 v i 37,56.
2. Cic phin doan dich chiit:
- Cin djch chiet trong phin doan hexan v i trong ethyl acetate diu cd tic dung ha glucose huyet vdi eie lieu v i tai cie thdi diem nghien ciru. & thdi diim 2h v i 4h, ein dich chiit trong n-hexan cd tic dung manh cin djch chiit trong ethylaeetat.
- Cin djch chiet trong phin doan eloroform (0,034g/kg) v i butanol (0,021g/kg) khdng cd tic dung ha glucose huyit d thdi diim 4h sau udng thude.
Summary
The methanolic and some organic-solvent extracts from corresponding fractions (methanol, n-hexan, clorofoc, ethylacetat and buthanol) of the plant roots administered orally were evaluated for hypoglycemic effects In mice with Streptozotocln-lnduced hyperglycemia. The blood glucose levels were measured before and several times after the extract administration by a glucose meter (Johnson & Johnson). Methanolic extracts lowered the glycemla In comparison to the initial levels. The organic extracts (n-hexan and ethylacetat) showed hypoglycemic actions 2hs and 4hs after administration. The effects of n-hexan extracts were stronger than that ol ethylacetate ones (P<0.05).
Tai lieu tham khao
1. Ta V a n Binh, Benh dai thao d u d n g tang glucose m a u , W X e y hpc, (2006) tr 13-15, 24-28.
N g h i e n
CLFU- K y t h u a t
2. Tran Thj Minh, Vu D i o Thing, Nguyen Thj Hoang Anh, Tran Van Sung, "Nghien ciru thanh phan hoa hpc cay Choc mau (Salacia chinensis L.) thu hat tai Thira thien Hue", Tap chi Hoa hgc, (2008) s6 1/2008
3. Vu Ngpc Lp, (2006), " Nhirng dupe lieu co tac dung ha duang huyet va trj tieu dudng". Tap chi dugc lieu so 353, 9/2005, tr 7-8; s6 356, 12/2005, tr 6-8; so 357, 1/2006, tr 5-6-34.
4. Lenzen S. (2007), "The mechanisms of alloxan and streptozotocin - induced diabetes", Diabetologia.
2008 Feb; 51(2):216-26 . Epub 2007 Dec 18
5. Yoshikawa Masayuki (2003), "Biological activities of Salacia chinensis originating in Thailand:
the quality evaluation guided by x-glucosidase inhibitory activity", Journal of the Pharmaceutical Society of Japan 2003; 123(ia):871-80.
6. Akinobu Kishi, Toshio IMorikawa, Hisashi IMatsuda va Masayuki Yoshikawa (2003), "Structures of New/ Friedelane- and Norfriedelane-Type Triterpenes and Polyacylated Eudesmane-Type Sesquiterpene from Salacia chinensis LINN. (S.
prinoides D C , Hippocrateaceae) and Radical Scavenging Activities of Principal Constituents", Chem Phanv Bull, (2003) vol.51; No.9, page.1051-1055 7. Pham Hoang Ho - Cay co Viet Nam - quyen 2, Nha xuat ban tre, trang 158.
Nghien ciru anh hu'frng ciia vien nang 10a - trjfluoro methyl hydroartemisinin (TFMHA) tren phan xa co dieu kien 6* chuot cong tr^ng
Tnrong Van Nhu', Nguyen Thj Minh T h u \ Nguyen Manh Hung', Nguyen Kim Phu'gfng^ Do Thj Phuong ^ va CS Vien Sot ret - Ky sinh triing - Con trimg Trung irang
' Vien Dugc lieu - Bg Y te
n g h i i m tien l i m s i n g d P h i p va Viet Nam '^•^•''•^•1.
H i i n nay, TFIVIHA dang t i i p tuc d u p e phSt trien d giai doan tien l i m s i n g , v d i e i e thir n g h i i m thude t h i n h pham d dang v i i n n i n vS v i i n nang. Be t i i p tuc p h i t trien mdt thude sdt r6t mdi, ed kha n i n g chdng k h i n g , ddng thdi gdp p h i n nghien eiru tinh an t o i n cua thude, nghiin ciru n i y d u p e t i i n h i n h n h i m d i n h g i i anh h u d n g cua TFMHA d i n mdt sd chirc n i n g hoat dpng than kinh trung u a n g cua chupt edng tring.
D6i tu-o-ng, vat lieu va phu'ang phap
Odi tuo'ng va vat lieu nghien cii'u:
Dpng vat nghien ciru:
- Chupt cdng t r i n g cdn non, dang tudi trudng t h i n h (90 con), 5-6 tuan tudi, trpng lupng 55 ± 10 g, khoe manh, khdng p h i n b i i t d u e - c i i , do Hpe v i i n Q u i n y cung cap, d u a c nudi dn djnh 2- 3 n g i y t r u d c khi tien h i n h nghien ciru.
Thuoc va hoa chat nghien ciru:
- Vien nang TFIVIHA 50 m g , do Cdng ty c6 p h i n D u p e p h i m trung u a n g II (Dopharma) san x u i t , dat t i i u chuan e a s d , Id 030708. Nguyin lieu l i bpt T F M H A do V i i n H o i hpc edng nghiip san x u i t , dat t i i u chuan c a s d .
Dat van dh
Do h i i n t u p n g ky sinh triing sdt ret khang thude dang n g i y c i n g gia t i n g va lan rdng t r i n t o i n t h i gidi, n i n v i i c nghien ciru v i p h i t trien e i c thude sdt r i t m d i rat d u p e quan t i m . Cho den nay, nhdm thude artemisinin da d u p e p h i t trien theo hai h u d n g : h u d n g thir n h i t l i phdi hpp vdi mpt thude sdt ret k h i e ed thdi gian b i n thai d i i han v i h u d n g thir hai l i tao e i e d i n x u i t cd boat tinh sinh hpe.
D i n xuat g i n fluor eua dihydroartemisinin d u p e c i c n h i khoa hpc Trung Qudc tdng hpp d i u tien v i o n i m 1987, cd hoat tinh cao ban tir 2 - 3 lan e i e hpp c h i t t u a n g t u nhung khdng g i n fluor.
Cudi t h i p ky 1990, Trung t i m N g h i i n ciru khoa hpe qude gia P h i p eiing vdi Vien Hda hpc cdng nghiip v i V i i n H o i hpe d i san xuat d u p e cae d i n x u i t g i n fluor eiia artemisinin '^'. D i n xuat 10a-trifluoro methyl hydroartemisinin (TFMHA) d u p e lua ehpn trong sd dd do ed h i i u lue tdt t r i n P falciparum nudi c i y v i P. berghei n h i i m d chupt n h i t t r i n g , vdi h i i u luc diet KST manh v i nhanh han artemisinin, ddng thdi dpc tinh t h i p ban so vdi c i e d i n xuat g i n fluor eiing d u p e tdng hpp. N g u y i n lieu TFMHA d i v i dang d u p e thir
32
