• '^HOCyieTMAMTtfW-THANGJZ.soBACBier-^Olii
^*. CUA TRA HOA VANG THU HAI TAI BA CHE - Q U A N G NINH
mis,
« « • ,
sfc* T 6 M T A T
i*l» m hot, vang ( & « e / & sp.) 14 m« loai duoc ' « « ll?u quy vm nhiau tac dyng sini, lioc qua„ ti;,„g
nllir h? hayh ap, 1,, dirong huyit, gian, cliolesteroj man, giim mS mau, cliang u buou ling cuimg I,? miln djch va keo dai tu6i thg
" ' f ^ S " ""• ^"'' "'•''"' * ^'^' "^^ '"*' "^'y "4y
"Sfllgg^dupc quan tam nghiin cihj day dij vi th^ic '^- vfc'fhinh phan hoa h,)c va lac dgng sinh hpc.
_ Tu- khia: Camellia chrysanlholdes Hung. T.
Chang, Trt hoa ving, d6ng te bao ung thu, d^c 1^
' bio
Ng8 Thj Thao*, Nguyin Thi Ha L y " , Thi Linh Giang***
Hoang Qnjuh Hoa***, TrSn Vin On***' blood, anti cancer, improvement of immune system and anti aging. In addition, Tra hoa vang lias been known as a highly economicvaluable plant. However, this plant has not been fclly researched In botanical characteristics, chemical components and biological effects in Vienam.
Ktry words: Camellia chrysanlholdes Hung.
T. Chang, golden lea, cancer cell lines, Cytotoxicity
" ' SUMMARY nm
1^1 MORPHOLOGICAL STUDIES, y,!, DETERMINATION OF POLYPHENOL,
f.4 EGCG AND TRY SOME IN VITRO t BIOLOGICAL EFFECTS OF GOLDEN TEA
* HARVESTED IN BA CHE-QUANG NINH
*-_ Golden Tea (Camellia sp.) has been known
•; as a prospective medicinal plant widi a lots ofbiological effects such as hypotension, ,i, hypoglycemia, controlling cholesterol level in 69 'Truing cao ddng yti Qudng Ninh
» "CingiyCPTraphaco
*• * Truing Dgi hpc Dupc Hd Npi 1-* Chill trich nhifm chinh: Ngd Thj Thao :••'- Email: [email protected]
l^Eiy nhjn bii: 6.9.2016 Ngiy phin bien khoa hoc: 14.11.2016 Ngiy duyel bii: 30.11.2016
I.OATVXNOJ
Ti<n thi gicM, chi Camellia L. c6 t « hon 200 loii, frong do nhiju Ioai c6 hoa miu vang. Nhieu nghien ciiu chi ra rjng cdc how chdt trong li, hoa Tri hoa vang c6 tic dyng ha huyit ip, h» duong h u y ^ h , cholesterol, h?^m9 miu, chong u buiiu, tSng cuimg h^
mien dich va keo dai tuoi thp [4], [6-8]. 0 ViSt Nara, theo udc tinh ci5 khoang gdn 20 loai khac nhau thupc chi Camellia L. [1], [3].
Cay Tra hoa vang Idn ddu tien dupc ngu6i Phap phat hi$n 6 miSn Bdc nuoc ta nam 1910, dupc xem la ngu6n gen tf nhiSn v6 cimg quy hilm. Tuy nhi&i, cho dSn nay cfc c6ng tac nghita ciiu vj Tri hoa ving trong nu4c con chira ddy du. Hifn t^ii, cic loii niy mcri chi dupc quan tam djn gia tri cinh quan, c6n cic gii tri vd sinh hpc, dupc hpc chuj ilupc quan tam va khai thac nhiju. Do c6 gii tri kinh tj cao nen ciy Tri hoa vang di vi
145
HNKH Y DXjqC - KY NlgH 50 NAM NGAY TRUY&N Tti^NG TRlfCyNG CAO D A N G Y T H A I N G U Y £ N (1966-2016)
dang dirgfc khai thac can kiet va co nguy co hi tuyet chung trong tir nhidn. Tuy nhien, viec nghien cuu va tim hieu ve Tra hoa vang 6 Vipt Nam con chua duoc toan dien va ddng bp. Do do, vi6c nghien curu vk d5c dilm thuc vSt, thanh phSn hoa hpc va mot s6 hoat tinh sinh hpc ciia cS.y Tra hoa ving la clhi thifit, gop phSn tim hifiu va khai thac cac gia tri cua losi cay thuoc quy nay.
II. TOITirONG VA PHirOfNG PHAP NGHIEN OJU 2.1 DSI tugmg nghiSn cihi
Trk hoa vang dugc thu hai 6 Ba ChS - Q u ^ g Ninh vao thang 3 nSm 2015, d2 dupc xit \f v i luu trfl tiSu ban tgi B5 mon Thvrc vSt - Truong Dai hpc Duoc Ha Npi (S6 hi$u tieu ban:HNIP/18146/15).
2.2 Phuang phdp nghiin cuu Phwang phdp nghien cuu thuc vgt - Mo ta dac difim hinh thai bang phuong phap md t i phin tich [2].
- Lim tieu ban vi hpc than, l i theo phuong phip cit, tay, nbu$m k6p.
- Quan sit vi chyp inh bpt dupc lipu l i vi thin kinh hiln vi tich hpp camera.
Phuang phdp nghien cthi thdnh phdn hda hgc
- Djnh tlnh nhom chit polyphenol trong Ii Tri hoa ving bing cac phan ung h6a hpc
dac tnmg: voi thuoc thii FeCb 5%; NaOH 10%; gelatin 1%
- Dinh lupng ham lupng Polyphenol ti phin trong l i Tri hoa ving bing phui phap do quang, theo TCVN 9745-1:2013, sii dimg phan ung t?io phiic miu vdi thuoc Hiis Folin - Ciocalteu va do quang 6 bu6c song 765nm.
- Dinh tinh EGCG trong li Tra hoa vang bang HPLC
- Dinh lupng him lupng EGCG bang HPLC theo tifiu chuin qu6c gia TCVN9745*
2:2013, six dvmg cOt Nucleosin C18, vdi pha dpng Acetonitril - Acid Acetic - EDTA, t^i bu(5rc s6ng 278nra, vdi t6c dp dong 1.0ml/
phiit.
Phuang phdp nghiin cuu tdc dung sinh hgc
- Xdc dfnh hogt tinh gdy dgc mpt sd ddng ti bdo ung thu cua edy Trd hoa vdng
Tiln hanh thir vdicto ciia dich chiet methanol theo phuong phip cOa Monks (1991) tren 5 d6ng tfi bio (TB): TB ung thu vu (MDA-BA-231); TB ung thu phoi (LU- 1); TB ung thu da (SK-Mel2) ; TB ung thir gan (HepG2) v i TB ung thu d?i tiing (SW480)[5],[7],[8]
Khi nang uc che te bio s6ng klii c6 m^
chat thii sS dupc xie dinh nhu sau:
[OD(chat thiJr) - OD(ngiy 0)] x 100
% t l bio song s6t =
OD (d6i chung ira) - OD(ngiy 0)
% lie chi t l bio = 100% - % tfi bao s6ng sot
YHOC WET HAMTAP44a.THAMG12-SomrBier.20i6 - 2 M idc dtfng chdng oxy hia ctia edy Tra hoa vdng
' S - 1 * . Tron\l^^ (O>-l.*.s-OD„l,«)*100/OD«ii^^, (./o)
>«fe " ^ ° " * ' * ' - "''"A.i^.s- O0 hdp Ihu igl giing khong chua chit thtr Oflmifc- Bi hdp thu lgi giing chira chdl Ihtr likj
Nl. Kft-QUA NGHIEN Ciru j n j 5
Kk. 3.1 Vl thi« vat " ™ ' " " " ™ ^ - ^"""S hoa rat ngin, 311 Bi^tiim hint. ,•,- ^ ' ^ " '**°* ^^ ""^"^ " bic 9, m}t
« . S T n ' . r ^ "tf: "• " ^ - " ' " ™ =^8 " ^ »^"8. xjp thinh L dSy phu - u« ; k T K ! • ' " " • " ^ " ^ "• " - * ^ " - '^''^todu^lcn.ren.diiO -76 i * » h " ' K : ' " ' ™ ' " ' • """^ ™- " *«^ 5. 3 dai ddu 06 tei mi, ngoL
< 4 0 •^^"B nh»,, phu nhieu long tring, rain; nhin trong co long vi ISng mi dM; dii 6 7 d^
.. ZlZT"^''^'"'^-'"'' ™ " > » - a n g a d u « . c J h o a l . c i c ^ . Z c t L i r T A " '^' " ^ ' " ^ ' ° " -••^ vio v6ng nhi ngoii. Tting t h U . ™
' c^Ti. . a T'~ " ' " " • ' " ''•'•''•* °8oiinhln.raS,t,ongc616ng.atringdinh6 M m iam 4 mit trln. nhin. Phien la (binh to) nua tr^n, bi, diu tCr tring 2, L g 3 t r ^ 6
t ^i;^ t T r ' - ^ r : ' " • "*' "-''•=™- '™8«l'-ddn,trang7djn U Jnglong. B^
' I r « f ^ g ' " 14. <^hS rSng nhat khia 6, dii 4 ram. r0ngl mm. B0 nhyy gjm 3 li
*' * ^ f » " ' ^ ' ' 8 . m 5 , d u a i r a a u . a n h n h „ , gjo, xanh nh,,. Qui chua ftdy
.^8n, tt,u6ng c6 nhieu dita ,uyjn miu den; Qua cic djc dijra ma ti. dji ohiju v6i
nfch li. duftng kmh cia hoa khi no khoang chrysgnlhoiies Hung T Chang
147
HNKH Y DU-gC - K t NIEM 50 NAM NGAY TRUYfeN T H 6 N G T R L T O N G CAO D A N G Y T H A I NGUYfiN (1566-2016) 'M
MBftag^MCT
?y jBjL \ ^ w H
^^^yfiJL^y
^^^m^r^PHHHRl
| ^ U B B ? M H ^ S | ^ ^ ^ ^ ^ B H H S L ^~.. lawJ
K^-"*-^^^ J|K:'^'«
^feK -'^^isdmSs^lk.^^'?^^
F^^^^^^f^^ff^^U
^•u^HHIH^^Kl^^H^HB^^taii^HS
Hinh t. Dac diem hinh thii m l u Camellia chrysanthoides Hung T. Chang.
1. Todn edy; 2. Choi bao Id; 3. Cdnh non phu long; 4. Ld; 5, 6. Hoa nguyen v^n; 8. Ld ddi; 8, 9. Mdt Irong vd ngodi ctia trdng; 10. Bg nhi;}}. Bp nhyy; 12. Bdu cdt ngang 3.1.2 Dpc diim vi phdu thdn: go, bit miu xanh.i/6e cdp hai (5)gbm cic t^
Quan sit tifiu bin vi phlu thiy than ciy bao hinh trdn, xfip thinh diy xuyfin tam. Go c6 tiet di?n tr6n, tir ngoai vao trong g6m cic cdp hai(3)li&n tyc thinh m^t cQng quanh mfi phan sau; Bieu 6/(1): g6m mpt lop t^ bio mim rupt; t^ biogS hinh da giic hojc chff hinh chft nhat tuong d6i deu nhau,phia ngom nh^t, kich thudc khong d^u phfin bo Ak\i c6 phu lop cutin. Md mim vd (2): g5m nhifiu trong viing mo mem go. Rai ric trong iibe ldp tfi bio da giic, kich thucrc khong dfiu, sip cip hai l i cac Ihi cung. Md mim rupt (4):
xkp Ipn xOn. Cung md cung (6): gom 2-3 Idp g6m cic t^ bio da giic, xep Ipn xOn.
te bio, kich thuoc khong dfiu, thanh diy hoa 148
SS* YHlX:VltTHAIITAP449.TMANB12-SonArniCr-
Chu thich Bieu bi Mo mem vo Go cap 2 Mo mem ruot Lite dp 2 Cung mo cling Long che cha
Hinh 2. Die dilm vi phlu thin non
3.1.3 B0c aim viphdu Id:
^ + Gin giita: gin Iji ci 2 mjt, mi, duoi loi rii. Til ngoai vio frong gom: Biiu ii{l) gom mp, hing ,j bio hinh chO nha, kich lhu6c khong dJu, co phu mO, lop cutin mSng. Md mim(.2) gjm cic lop ,j bio hinh ti*n. xep Ipn xOn dj ho nhidu khotag gian U0.M0 ctrng{3) gjra 2-5 lop ,j bio hinh da giic. Uiinh diy hSa go,sip xJp tao Uiinh rap, cung bao quanh bo libe-go. Bo libe-go co go
o tten, Iibe phia du6i;//6e(5) g6m nhiju ldp ,e bio hinh da giac kich tfiudc dJu, xJp I$n xpn tfiinh tog dim; mgch gi(4) hinh da giic hay wdng xjp tfiinh dgy xen lln vfti rao raem go (te bio hinh vuong hay da giic)..
Nira tii tic ttong mo mjra li ihi cung(6) kich tfiuftc Iftn nhinh nhpn, da hinh dsng.
Tinh tfie caici oxalat hinh ciu gai niraft mo mem vi Iibe.
Chli Ihich 1. Bieu bi 2. Mo mim 3. Md Cling 4. Mach gd 5. Lite 6. The cimg
Hinh 3. Die didm vi phlu gin giSa U
WJ
HHKH Y DUpC - K» NIgM 50 N A W N G A Y T R U Y £ N T H 6 N G T R U I N G CAO P A N G Y T H A I NGUYfiN (1966-2016)
+ Phiin Id: Bieu bi {l)g,Qm mot lop tfi bao hinh chiJ nhat, tfi bio bifiu bi trfin ldn hon te bio bifiu bi dudi; bifiu bi trfin co vich trong rit diy v i cutin diy; lo khi nbieu d bifiu bi duiM.
Md gidu(3) ghm mpt Idp te bio thuon dii xep xit nhau. Md khuyet{4) gom 8-9 ldp te bio da giic hoac tron, sap xfip I6n x6n. Nim rii ric trong mo mim(2) la cac tinh thfi canxi oxalat(Q"%
hinh cau gai va Ae cung da hinh dang, cd nhanh nhpn
Chu thich L Biiu bi 2. Mo mem 3. Mo gidu 4. Mo khuyet 5. llie cung 6. Tinh the caici oxalat
Hinh 4. DSc dilm vi phlu phi^n l i 3.2 Dpc mim bpt dugrc lipu:
Bpt l i Tri hoa ving miu xanh rfiu, cd miii therm dJlc trung cda tri. Cic d$c di^m chfnh quan sit dupc tii b^t t i Tri hoa ving bao g6m: minh m6 raem; tinh th^ canxi oxalat; minh mang miu; the cttag, bifiu bi mang lo khi; long che chd; minh m^ch xoin.
Chu thfch l.Mo mem
2. Tinh thi Canxi oxalate 3. Mdnh mang mdu 4. H0 dipp l^c S.Ldkhi 6. The cihig 7. Biiu bl chua 16 khi 8. Long che ch&
9,10.1} Mdnh mgch xodn
Hinh 5. D^c di^m vi phlu b9t dirge lifu T r i hoa ving
!S3j
»lt»|l5
Kin, tioti
Y HOC V i e r HAM TAP 449-THAMG 1 2 - s n BAC B E T - 2 n i 6
3.3 vi Ihinh phin hda hpc
hoa v b ^ c h " ^ Z^ ' f f " * ' " ' ' ' ^ ' ^ " ' • " " " ^ ""'"• 8 " = ' ^ '""• •<=' " * • <"°B « « noa vang co chua nhftra Poplyphenol
Djnh lupng Polyphenol bing phuomg phap do quang
+Xdc iinh khodng luyin linh: KJt qua khio sal dp n^-jn tinh dupc ttinh biy ft Bing 1 HiBB 1- Ket qua khao sat ndiiK d j tuyln tinh acid gallic
Cchuan (u^mU
Bp hip Uiu (ABS) 0.I3I 0.258 0.366 Phuomg Wnh hoi quy myjn tinh:y = O.OlOx + 0.041
He sft ttiong quan: R^=0 993
0.553
KJ, qui eh<> fliiy tro^g^;^^;^!^;^!^, 34, ^ buftc's6ngy65mn, dp hip Uiu v i n6ng dO acid gallic co su nrong quan chit che vfti he sd nrong quan R ^ 0 993
^ dinh id ldp lgi ctia quy Irinh: KJt qui dupc ttinh biy ft Bang 2.
^ ^ 2. Ket qua khio sat dy lap lai cua guy trinh
^ ^ ^ A o c dinh id Id}
^ K M B i " ! I| I
^ ^ Dft h i n thil rAR<:^ B j hip tfiu (ABS) Him lupng Polyphenol (%)
0,5023 0,347 6,922
0,5020 0,348 6,949 B? Ijch chuan:
0.5021
6,925 0.5021
0.350 6,993
0,5022 0,349 6,969 RSD = 3 . 4 1 %
0,5023 0.346 6.900 iT, — . =^^^^ R o u = j , q i yo
Ket qui khio cho tfiiy phuong phip cft do ISp Ijii chip nhin dupc vfti RSD= 3 41%
J-Xdc iinh ham lugrtg Polyphenol: D^a vio phuong ttinh duftng chuin. tini dupc him lupng Polyphenol trong miu Tra hoa vang li: 6,943%
Binh lupng EGCG bing phuong phip HPLC
^Xdc dlnh khodng tuyin linh: KJt qui khio sit dp ttiyjn tt'nh dupc ttinh biy ft Bing 3 Bing 3. Kit qua khao s i l n i n ^ dp tuvIn Hnh F.f:rf:
Cchuin f|ie/ran Dijn tich pic
(mAu.s]
28.752 302.8
57.504 583,5
86.256 926.3
115,008 1291.8 Phucmg nJnh hji quy toyJn tinh:y = 11.40x - 45.29
Hj so tuong quan :R^= 0,998
143.76 1588.4
3.4 vi tic ifng sinh hgc
151
HHKH Y DUCfC - KY NlEM 50 N A M N G A Y T R U Y £ W T H 6 H G TRl/dNG CAP B A N G Y THAI HGUYfeN (1966-2016)
Bang 4: Ty
Ihiy
(Hg/m 1 100 20
4 0.8
CEUipticm
10 2 0.4 0.08 ICso
e ox che sir p h i t triln ciia te bao ung thir tren 5 dong t l b i o ung thlE^S Ty le irc che sir phat triln
MDA-BA- 231 CA 93,4 6 48,7
9 10,2
3 4,20 2S.2 3
EL 98,4
9 76,2
6 49,6
2 19,4
4 0,47
HepG2 CA 98,4 3 46,8
6 16,4
6 10.2
6 24,9
5 EL 91,9
9 76,6
9 48,6
8 15,6
7 0,54
ciia t l bao trJn tirng dftng t l b i o ung thif
(%)
SW480 CA 73,2 6 40,2
4 14,5
3 4,08 36,8 1
EL 87.1 6 75.9
3 45.9
8 19.0
6 0.56
SK- CA 88.3 3 13,6
2 9,98 0,43 64,4 6
VleI2 EL 85.5 6 78.8
3 49,3
6 18,5
6 0,51
LU-I CA 79,6 5 21,3
7 5,30 6,07 53,1 3
EL 87,2 6 68,8
5 50.1
3 26.2
4 0.47
j
f
1
i
Nhpn xdt: Mau nghien curu the hi§n miic ho^t tinh giy d^c tfi bio khic nhau tren dc d6ng tfi bio khic nhau. Ket qua ICso trfin dong te bao ung thu gan; ung thu vu vi ung ihir trvtc tring tuong dng la 24,95; 25,23 v i 36,81. Thii nghi?m cd tic dvmg th^p trfin d6ng tk hki ung thu da (SK-MeI2) v i ung thu phoi (LU-1) vdi gii tri IC50 tuong ung l i 64,46 vi 53,13.
Cac k^t qui tren la chinh xie vdi i^ > 0,99.
3.4.2 Thu tdc d^ng chong oxy hda cua c3y trd hoa vdng
Khi nang trung hoi goc t^ do ciia cao khd la Tra hoa ving dupc trinh biy trong Bing 5.
Bang 5: Kha nSng trung hda fi6c oxy h6a tg do cua mau thfr Njng dp
(ng/ml) 200 100 50 10 SCsn
GiittiSA(%) Mautfiii
82,01 77,85 61.64 9,13 42,43
N jng d j (WR/ml)
100 50 10 2
GiittiSA(%) Ascorbic Acid (Vit C)
85.37 83.14 59.96 25,46 7,58 Nh^n xil: Mau tfitr cft ,4c dyng yju vfti tj IS trung hoi goc tu do dat dupc l i 9,13% 4 nftng dp 10 pg/ml; 82,01 % ft nftng d j 200 pg/ml.
IV. BAN LUAN nhilu mSu cft djc dijm tucmg dJi gi jng nhau Ve thirc vil: Mlu nghien ciiu chua xie nen ngoii phuong phip mo , i hinh thii vi djnh dupc loii do chua dii dtt liju ve hinh giii phau, cin tfln hinh ,hjm vijc nghiin tfiii cia ,it c i ca quan sinh sin nhu qui, hat ciiu da dang di ttuyen dj nghiin ciiu sSu hotl '' Tuy nhiln, ttong tfiyc te tfiu mlu, do cft vj phan lo»i loii v i dufti loii. '
YHOCVlJTWAMTAP449-THAllCii2-s6BACBI^.»ne Ve Ihanh phdn hod hgc: Nghien ciiu da
xdc djnh dupc hira lupng polyphenol toin phin l i 6,943% v i hira lupng EGCG l i 0,40% ttr dich chijt toin phin metfianol. Xet tten khoing tayjn tinh, phuong phap co dp lap Iji cao tten mau fliu vfti RSD l i 3,41%.
Ket qua dinh lupng cho fliiy cft tfij ap'dung phuong phip tten d j dinh lupng Polyphenol vi EGCG trting tti hoa ving.
Ve idc dung sinh hgc: Nghien ciiu oijng gftp phan dinh gii khi ning iic chj sir phat trijn cOa mjt sft dftng te bio, tfij hien qua ty I j tl bio sftng giim co y nghfa vfti cic njng dj khic nhau, cho tfiiy Tri hoa ving cft khi _ j 4 c chj sir phit ttijn doi vfti nhiju loai bio ung thu, vfti timg nong d j vi doi vfti timg dftng tJ bio ung thu khic nhau tfii a c dung lie chJ cOng khic nhau. Tuy nhien, vfti nghiJn cuu niy chi dimg Iai ft viec thii dich chiet toin phin nen tte dyng chua cao. Vfti tic dyng chong oxy hoi, mau nghiSn ciiu cOng fte hijn a c dyng ttung hoi gjc tv do nhung vfti 5? | j fl,ip (9,13 »/. ^ „^„^ ^. ^^^
ipng/ml).
V. Kfr LUAN
Di mft a chi ,ie, vj djc diJm hinh ,hdi Tri hoa ving tfm hii ijii huyjn Ba Che, Unh Quing Ninh vi so b j xac djnh ,Sn khoa hpc eta miu nghiSn ciiu li Camellia chrysanlholdes Hung T. Chang., Theaceae.
Ba mo a d8c diem ciu ,?o giii phlu cua Ihin vi l i Tni hoa ving Ba che (Quing Ninh), gftp phan vio vijc nhiin bij, va phan lo9i cic loji Tri hoa vang.
lid
Da dinh lupng iftng him lupng polyphenol cft ttong la mlu nghien ciru li 6,943% bing phuong phip do quang, sir dung phan irng t^ miu vfti tfiujc tfiu Folin- Ciocal,eu, voi he sft hJong quan R^=0,993, cft dp lap lai RSD=3,41%.
_ Da xie dinh him lupng EGCG cft ttong man nghiSn ciiu la 0,40% tfieo phuong phip HPLC, voi h j sft Krong quan R^=0.998.
Da tfiii a c dyng gay dftc , j bao trin mjt so dftng tJ bio ung thu tfivrc nghijm (in vitto). dich chiJt rt, Ii Tri hoa ving (Camellia spl.) tfij hien ,ac dung uc chj tnmg binh dfti vfti ung fliu gan (Hep-G2)- ung tfiu Vli (MDA-BA-231) vi ung ihu ttuc ttang (SW480) vol cic gii tti IC5„,uong ung la 24.95; 25,23 vi 36,81; ,ic dyng tfiip ttSn dftng ts bio ung thu da (SK-Mel2) va ung tiiu phfti (LU-1) vfti gii trj ICo nrong iing li 64,46 vi 53,13.
B i tfiir a c dyng ttung hoi gftc t^ do cia dich chiJ, Id Camellia spl.trit, mft hinh DPPH cho kJ, qui cft tic dung yju vfti ty Ij 9.13% ft nftng d j mlu tfitr 10 pg/ml; 82.01 % ft nftng d j 200 pg/ral.
TAI U l u THAM KHAO
1. B S Ngjc Quy, NguySn Kin ph„„g (jj57j^
Cdy che Vlfl Nam, NXB. Nftng Nghi?p, Ha Nji.
2. Nguyfn Ba (2010), Hinh ihdi hpc Ihtfc vgl NXBGiao dye.
3. VS Vin Chi va Trin Hyp (1999), Cdy co ci ich A Vlfl Nam, NXB Giao dye. Ha nji.
"*' ^.? T ' ' ^ ' ' " ' ^ ° ^ " " ' " 8 Tiln v4 cs (2001),
"Cdc hgp chdt cd trong che vi mpl si phuong phdp phdn lich Ihing dung Irang sdn xudt che a Vift Nam ", NXB. Nftng Nghifp
HNKH Y DUqrC - K t NIgM 50 KAM NGAY TRUYfeN T H O N G TRlJdNG CAO B A H G Y T H A I W G U Y £ N (1866-2016)
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** B?nh vi4n Trung uang Thdi NguySn Chju trach nhi^m chinh: Nguyin Ba ThSng Email: [email protected] Ngay nh^n bai: 5.9.2016
Ngiky phdn bi?n khoa hpc: 14.11.2016 Ngay duy?t bai: 30.111.2016
Nguyin Bi ThSng*, NguySn Minh Chung*, LS Thj Ha Giang*, Phan Thanh Nhung**
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