• Nghien ciyu - Ky thuat
Danii gia tac dung cliong viem ciia cao cliiet xuat
r r
tir la cay cop Bac Bo {Kopsia tonkinensis Pitard, ho Triic dao Apocynaceae)
D 3 Van Binh, Trieu Duy Di^t, Hoang Vidt Dung'
HQC vien Qudn y ' E-mail:vietdungk85@yahoo. comSummary
In continuation of our previous report on the acute toxicity and analgesic effects of the leaf extracts of Kopsia tonkinensis Pitard, the anti-Inflammatory aeitlvlties were experimentally valuated by Jane - Piecini - Winter models. The extracts from the leaves of K. tonkinensis exhibited inhibitory actions on both acute and chronic inflammation at dose of 8 g/kg body weight, but did so on the increase of vascular permeability only at a higher dose of 20 g/kg body weight. These effects all proved weaker than those of Ibuprofen at the experimental doses.
Keywords: Kopsia tonkinensis Pitard, anti-inflammatory aeitlvlties.
£)at van de
Cay cdp Bae bg {Kopsia tonkinensis Pitard) phan bd kha han che vd dupc thdy d mgt sd noi nhu Hda Binh, Thanh Ba - tinh Phii Tho, Vudn qudc gia Cue Phuong d nudc ta va Vdn Nam - Trung Qudc. Cho ddn nay, chua cd tai ligu nao cdng bd vd tde dyng sinh hpc cua cay ndy. Cf Vigt Nam, chi cd 2 lodi khde eung chi vdi cay edp Bae Bg duo'c sir dgng Idm thudc Id cay trang tay (Kopsia fruticosa) vd cay trang nam {Kopsia pitardii Merr.)^^. 6' bai bao trudc, chiing tdi da thdng bao vd dge tinh cap vd tde dyng giam dau ciia cay cdp Bae Bd. Trong bai bao nay, chOng tdi tidp tuc thdng bdo vd tde dgng chdng vidm eiia cao chidt xuat tir Id cdy nay.
Doi tu'O'ng, nguyen iieu, thiet Phu'O'ng phap nghien ciru
Doi tup'ng nghien cii'u
Chugt nhat trdng ddng Swiss trudng khde mgnh, ca 2 gidng, trpng lupng 20 chudt edng trdng ddng Wistar trudng khde mgnh, cd 2 gidng, trpng lup'ng 200 Ddng vgt thi nghigm do Ban chan nudi vign Quan y eung cap.
bi va
thanh,
± 2 g ; thdnh
± 2 0 g . Hoc
Nguyen lieu
+ La cdy cdp Bae Bg thu hdi tai Kim Bdi - Hda Binh vao thang 12/2005 da dup'c TS. Trdn Thd Bdch, Vign Sinh thdi Tai nguyen vd Sinh vat Vigt Nam, xde d\nh ten khoa hpc la Kopsia tonkinensis Pitard, hp Triic ddo Apocynaceae.
+ Cao Id edy edp Bac Bp (CKT): Id loai cao 2:1 dupc bao chd theo phuong phdp ndu cao duo'c lieu hudng dan trong DDVN 3 '^'.
Hda chat
Natri clorid, acid aeetie, carageenin: dgt tidu chudn thi nghigm ciia Bg m5n DudC ly - Hpc vign Qudn y. Thudc ddi chieu: Ibuprofen.
Thiet bj, dung cu
Mdy cdt quay chdn khong (Buchi - Thuy ST);
can phan tieh Mettler (Thgy 87, dp chinh xac 0,1 mg); may do dp phu viem ehan (Ugo Basil)...
Phuwig phap nghidn ciru
Dinh gia tic dgng tre chd tang tinh thdm thanh m^ch
Tien hdnh theo md hinh ciia Jayne va egng sy*^. Dgng vgt nghien cu-u Id chugt nhat trlng dyp'c chia ngau nhien thdnh 3 Id (mdi Id 12 con):
+ Ld 1 (Id eho'ng): udng nude mudi sinh ly vdi lieu 0,1 ml/10 g trpng lup'ng co the (TLCT).
TAP CHi DlTQC HQC - 07/2013 (SO 447 NAIM 53)
• Nghien CCFU - Ky thuat
+ Ld 2 (Id dOng thude nghien ciru): udng CKT lieu 20 g/kg TLCT.
Ld 3 (Id dung thudc ddi chidu): udng ibuprofen lidu 0,8 g/kg TLCT.
Cho dgng vgt diing thudc 3 ngdy lidn tuc, Sau khi diing thudc d ngdy thO- 3 d u p c 10 phut, tiem tTnh mgeh dudi chudt dung djch xanh Evans. Sau 5 phut, tiem phiic mac acid acetic.
Sau khi tidm acid acetic 60 phut, gidt chugt, m d d bung, hut toan bp djch trong phOc mac. Djch nay d u p e ly tam v d do mgt dg quang trdn mdy do quang d b u d e sdng 515 nm, xde d|nh ndng dg chat mdu thoat ra khdi Idng mgch.
Dinh gii tic dung u v chd phu viem cap Tien hanh theo md hinh ciia Piecini vd cpng sy"^. Ddng vgt nghien ciru la chupt cdng trang dupe chia ngSu nhien thanh 3 Id (mdi Id 12 con):
+ Ld 1 (Id chirng): udng n u d e mudi sinh ty vdi lidu 0,4 ml/100 g trpng lupng co the (TLCT).
+ Ld 2 (Id diing thude nghien ciru): udng CKT lidu 8 g/kg TLCT.
+ Lo 3 (Id dung thudc ddi chidu): udng ibuprofen lieu 0,56 g/kg TLCT.
Cho chudt udng thudc 3 ngdy lidn tgc. &
ngdy thir 3, sau khi udng thudc 1 gid, gay phu viem cap bang edeh tiem dung djeh carageenin 1 % vdo d u d i da gan bdn chdn chugt. Do the tich ehan chugt bdng dyng cy chuydn dgng tgi cac thdi diem 0, 1, 3, 5, 7, 24 gid sau khi gay
phu viem cap. Dp tang thd tich chan chugt d u p c tinh theo cdng thirc:
AV = V, - Vo
Trong dd: Vo v d V, lan lupt Id thd tich chan chugt trude va sau khi gay viem.
Tac dung chdng vidm ciia thudc d u o c danh gia bdng kha nang ire chd phu viem, cdng thirc tinh nhu sau:
l% = (AVc-AV,)x100/AVc
Trong dd: AVc va AVt ian lupt la trung binh dp tang the tieh ehan chugt d lo chirng vd Id diing thude.Dinh gii tic dung u c chd viem man Dirge tidn hdnh theo md hinh cOa Winter vd Porter'''^ Dong vgt nghien cuu la chudt edng trdng dupc chia ngSu nhien thanh 3 Id (mdi Id 12 con):
+ Ld 1 (Id chirng): udng n u d c mudi sinh ly vdi lieu 0,4 ml/100 g trpng lupng co thd (TLCT).
+ Ld 2 (Id diing thudc nghidn ciru): udng CfCT lidu 8 g/kg TLCT.
Ld 3 (Id diing thude ddi ehieu): udng ibuprofen lieu 0,56 g/kg TLCT.
Gay viem mgn bdng cdch cay vien sp'i amian da dupe tam carageenin 1 % d da gdy ciia chudt.
Sau khi eay viem spi amian, cdc chugt dupc udng nudc mudi anh ly hoge udng thudc tnDng 7 ngdy.
Den ngay thu 8, tidn hanh gidt chugt, bdc tach khdi u hgt va can tuoi bdng can phan tich.
K e t q u a v a b a n l u a n
Tac d u n g i>c che tang t i n h t h a m thanh mach mac tree cua CKT Bang 1 : Anh hwdng cda CKT lin ndng do chit miu thoit ra khdi long mach
Lo nghien ciru Ndng dip chat mau (pg %} Ty le u-c che (%) Lo chirng (NMSL)
Lo dCing CKT Lo dung Ibuprofen
P2.i<0,05 P3.1<0,05 p2-
3<0,05
Nhin xet: Ket qud bang 1 cho thdy, d mue lieu 20 g/kg TLCT diing theo dudng udng cd tde dyng ire chd tang tinh thdm thdnh mgeh rd rgt (ty Ig uc chd dgt 50,68%) so vdi Id chirng, s y khdc bigt cd 9 nghTa thdng ke vdt p < 0,05. Tuy
nhidn, tac dung ire chd tang tinh tham thanh maeh cua CKT (ty lg ire ehd Id 50,68%) ydu hon so vdi tde dyng ndy ciia thudc ddi chidu (ty lg ire chd Id 65,58%) d mirc lieu nghidn ciru, s y khdc biet ed y nghTa thdng kd vdi p<0,05.
TAP CHI DlTOC H O C - 07/2013 ( S 6 447 N A M 53)
Nghien ctru - Ky t h u i t
Tac dung LTC chd phu viem cap
Bang 2: Anh hwdng cua CKT len thd tich ban chin chuot
MO'C dg wc che phi viem & cac thm diem sau dieu trf
sau gay Lo chimg (1)- viem (^Yc)
Lo dung CKT (2) L6 dung Ibuprofen (3) Tyleuc che
phd vidm % AVc-AVt
TyieOc chi phu viSm %
l g i *3 giiy 5 gia 7 g i i 24 gid' P
1,5 2,5 3,7 4,1 2,4
1,1 1,8 2,7 2,9 1,8
0,4 0,7 1,0 1,2 0,6
26,66 28,01 27,04 29,26 25,02 P2-1,3-1,3-2< 0,05
1,1 1,7 2,4 2,6 1,6
0,4 0,8 1,3 1,5 0,8
26,66 32,04 35,13 36,58 30,01
Nhin xet: Ket qud bang 2 cho thay, tgi tat ca ede thdi diem do, thd tieh ban chan chupt ciia ca Id dung CKT va Id dung thudc ddi chidu deu giam so vdi Id chirng, sy khac biet cd y nghTa thdng kd vdi p
< 0,05. Tac dung ire ehe phii viem manh nhdt trong Tdo dgng u>c che vidm man
Bang 3: Anh huo'ng cua CKT len trong luang u hat
khoang thdi gian ttr 3 - 7 gid d ca 2 nhdm diing thudc. Tuy nhien, tac dgng ire chd phii viem cap cua CKT ydu hon so vdi tde dgng ndy ciia thudc ddi chidu d mirc lieu nghidn ciru, sy khac biet cd y nghTa thdng ke vdi p < 0,05.
STT Lo nghi§n cwu
Trgng Iwgmg trung binh Ty le giam trgng Iwgng u hat u h9t (%) Lo chi>ng (NMSL) 12LS dCing CKT 12 Lo diing Ibuprofen 12
P3.1<0,05 p2- 3<0,05
Nhdn xet: Ket qud bang 3 eho thay, trdn md hinh gay ire ehd u hgt, CKT d mire lieu 8 g/kg TLCT theo dudng udng ed tac dung gay giam 18,2% so vdl trpng lupng u hat so vdi Id chirng, sy khae biet ed y nghTa thdng kd vdi p<0,05. Tuy nhien, tac dgng ire ehd u hgt cua CKT (ty Id giam trpng lupng u hat Id 18,21%) ydu hon so vdi tde dgng ndy eua thudc ddi chieu (ty Ig gidm trpng luo-ng u hat Id 32,23%) d mirc lidu nghien euu, sy khde biet cd y nghTa thdng kg vdi p<0,05.
Ket iuan
C/ mde lidu 8 g/kg TLCT, CKT cd tde dgng chdng vigm tren hai md hinh ire chd vidm cap va mpt md hinh ire ehd viem mgn. Tuy nhien, CKT chi the hign tde dyng ire ehd tang tinh tham thdnh mach d lieu 20 g/kg TLCT theo dudng udng. Tde dgng chdng vidm eiia CtCT trdn cac 42
md hinh danh gid deu ydu hon so vdi tde dung ciia thudc ddi chidu ibuprofen d lieu nghidn ciru.
Tai lieu tham khao
1. Bp Y td (2002), Dwac dien Vi$t Nam 3, Nxb. Y hpc, Ha Ngi, Phu luc 1.1.2. V5 Van Chi (1997), TCr dien tht/c v$t thdng dicing.
tap 2, Nxb. Khoa hpc vd k? thuat, tr. 1497 - 1498.
3. Jayne A., Sertie A. (1990), Anti-inflammatory activity and subacute toxicity of artemetin, Pianta Medica, pp. 36 - 40.
4. Winter C. A , Porter G. C. (1957), Effect of alteration in side chain upon antiinflammatory and liver glycogen activities of hydrocortisone esters. J. Am.
Phann. Assoc. Scientific edition 4Q. pp. 515-519.
5. Winter C. A , Risely E. A , Nuss G. W. (1962), Carrageenin - induced edema in the hind paw of the rat as an assay for anti-inflammatory drugs, Proc.
Soc- Exp. Bbl. Med.. 111 (6), pp. 554 - 547.
T ^ CHi DlTQC HQC - 07/2013 (S6 447 NAM S3)
