DI TRUYEN - GIONG VAT NUOI
DA DANG DI TRUYEN ADN TY THE CAC GIONG CHO BAN DIA TAI HA GIANG VA LAO CAI l>mrp
Pham Thanh Hdi^* vd Biii Xuan Phuong^
Ngay nhgn bai bao: 10/03/2015 - Ngay nh$n bai phan bi^n: 26/03/2015 Ngay bai bao duge chap nhan dang: 30/03/2015
T 6 M TAT
300 mlu sinh hpc phSn tit cua 10 giong ch6 bin dja t?i Ha Giang va Lao Cai dupe thu th^p va phSn tich ADN, viing gen d-loop du^c ch(?n de phan tich vi nhung uu diem trong phan tich tinh da dgng di tniyen dpng vat. Ket qua thu dwjc cho thay co sy da d^ng ve d^c diem viing gen d-Ioop giiia cac giohg cho: H'Mong c$c duoi co 10 vj tri bien dm t^o thtinh S Haplotype (Hap), H'Mong dai duoi co IS v| tri bieh doi tao thanh 7 Hap, H'MSng Long dai co 9 vj tri bieh doi tgo thanh 6 Hap, Bac Ha c6 9 vi tri bi^h doi tgo thanh 7 Hap, Vi^t Dingo c6 16 vi tri bieh d6'i tgo thanh 7 Hap, Akita co 6 vj tr( bieh doi tgo thanh 4 Hap, Sharki c6 5 vi tri bien do'i tgo thinh 7 Hap, Sharpei co 7 vj tri bidn doi tao thanh 3 Hap, Dingo Lon c6 6 vi tri bi^n do'i tao thanh 5 Hap, Lai Ka c6 8 vj tri bien dSi tgo thanh 7 Hap. Khoang each di t r u y ^ giiia cac gi5ng cho nghidn cim tir 0,5% deh 0,7%. Khoang cdch nay cho thSy m5i quan h$ di truyen cua cac giong cho nghien cuu la kha gan gui va on dinh. Cci ba cay phat sinh chung logi cua cdc Hap thupc 10 giohg cho ban dja thu duc;K: a Ha Giang va Lao Cai co ban la giong nhau, phan anh cdc Hap khong d§c trung cho giohg cho ban dia nao va cac giong cho nay van co sy giao phm voi nhau, tgo n@n sy dan xen cac Hap giua cac gidhg.
Tit khoa: Da dq.ng di truySn, gen d-loop, cho bin dia Viet Nam ABSTRACT
Genetic diversity of Mitochondrial DNA of Vietnamese indigenous dog in HaGiang and LaoCai provincce
Pham Thanh Hai and Bui Xuan Phuong 300 tissue samples on 10 breeds of local domestic dog in Hagiang and Laocai provinces was collected and analized the D-loop gene for detecting the genetic diversity of this group. The result of analysis has shown that the diverse of gene D-loop between breed of dog in Hagiang and Laocai such as; Breed of H'm6ng shorttail there are 10 locations mutation and created 8 Haplotype (Hap), Breed of H'mong long tail there are 9 locations mutation and created 7 Hap, Breed of H'mong long hairs hort tail there are 18 locations mutation and created 7 Hap, Vietdingo-16/7, Akita-6/4, Sharki-5/7, Sharpei-7/3, Big dingo-6/5; Laica-8/7; Distance of genetic of domestic dog in Hagiang and Laocai variations between 0.5-0.7%. This distance of genetic has shown that they are very clotherly each other and sustainable. The 3 Phylogenetic tree of total Hap of 10 breed of Dog in Hagiang and Laocai were similar, it shown that the Haps not characteristic for any breed of dog, and all breeds of dog in this area recently have matting each other, so that creates combination between Haps.
Key words: Diversity of gene. Gene D-loop, Vietnamses indigenous dogs
' Trung tSm Nhi^t doi Vi^t-Nga
• Tac gia de li6n h^: CN. Phgm Thanh Hai, Vien Sinh thai Nhi?t doi, Trung tam Nhi^t doi Vi^t-Nga, Duong Nguyin Van Huyen, Nghia Do, Cau GiSy, Ha Noi. Difn thoai: 0975 976168. Email: phamhai.yun@gmail,com
KHKT Chan nuoi SO'10-2015 1?^)
1. DAT VAN DE
Ha Giang va Lao Cai la hai trong cac tinh phia Bac va Bac Trung Bp co thanh phan va ty 1? cac dang eho ban dja eao trong nuoc ta (Bili Xuan Phuong va etv, 2010). Vi?e bao ton va danh gia ve sy da dang di truyen cac giohg cho ban dia 6 day noi rieng ciing nhu a nuoc ta noi chung chua nhieu. Hien cac danh gia chi ve thanh phan khu h | cho theo huong dieu tra khao sat va tong hgp danh gia khach quan chua ehi ro duQC moi quan he giiia cac giohg voi nhau. Voi su phat trien cua sinh hpc phan tir, vi§c danh gia tinh da dang di truy&i bang ADN ty the dang rat pho bieh tren the'gioi cung nhu tai Vi?t Nam. Vi ty the CO h? gen rieng (mtDNA) co kich thuoe nho 15-20 kilobases (kb) chia lam 2 viing: vimg ma hoa va viing dieu khien (D-loop). mtDNA CO ca'u triic di truyen ra't on dinh, tai ban va di truyen dgc lap voi h? gen nhan (Wilson va ctv, 1985; Wallace, 1986; Clayton, 1991;
Gyllensten va ctv, 1991; Stoneking va etv, 1991). Voi nhiing dac diem tren, mtDNA dupe eoi la mpt trong nhirng cong eu pho bieh trong phan logi hgc, phan ti'ch lien hoa va khao sat da dang di truyen quan flie 6 dpng vgt CO xuong so'ng. Nghien euu eiia Rebekah va etv (2007) eung chi ra rang Viing (D-loop) thuong dupe sii dyng trong phan tich da dang di truyen va tie'n hoa eua loai cho nha Canis familiaris.
Vi^c six dung sinh hgc phan tii trong vi|c phan tich moi quan h? di truyen ciing la nhiing nghien euu bude dau doi voi loai cho eua nuoc ta. Ke't qua eua nghien ciiu ra't co y nghta cho cae nghien cuu, djnh huong ve bao ton, danh gia, nhan gio'ng nguon gen quy eua loai dpng v$t von gin bo vdi eon ngudi chiing ta tir rat lau nay. Day cung la nghien Cliu trong de tai cap nha nude trong suot ba nam qua da thu dupe mgt so'ke't qua.
2. VAT Ll|U VA PHl/ONG PHAP NGHIEN CU'U
2.1. V?t li^u nghien cuu
300 mau mo tai cua 10 giong cho ban dia tai hai tinh Ha Giang va Lao Cai dupe thu
thap bao quan theo quy trinh thu mlu sinh hpc phan tii. Mau mo dupe bao quan trong moi truang eon 90" dam bao khong lay nhiem hay pha t?p giiia cac mau. Cae mau thu dugc theo timg ca the duge lua ehgn co vj tri khoang each dja ly va quan he huye't thong xa nhau nha't qua kh^o sat tren thyc dia de dam bao tinh khach quan nha't. Chiing toi tie'n hanh phan loai eae gio'ng cho va danh da'u ki hieu nhu bang 1.
Bing 1: So lugng vi ky hi?u cac giong ch6 bin dia thu dupe d Hh Giang va Lao Cai Ten giong
H'Mong CQC duoi H'lvlong dai du6i H'Mong long dai Bac Ha Vi?t Dingo Alcita Siiarld Sharpei Dingo Ion LaiKa
So mau 120 20 20 20 20 20 20 20 20 20
Kv h i i u mia HCM20 HDl-20 HLl-20 BHl-20 VDI-20 AKI-20 SKl-20 SPMO DLl-20 I,Kl-20 2.2. Phuong phap nghien ciiu Tdch chiet va giai trinh tugm dich
Tach chief DNA tong so' bang bp hoa cha't thuang pham QIAGEN blood and tissue Kit (QIAGENJnc, Due). Cae bude thyc hi?n theo hudng dan eua nha san xua't.
Nhan ban do^n gen co kieh thuoc khoang 600 base thupe vimg bie'n doi cua gen D-loop bang ky thuat PCR sii dung h6n hpp PCR Taq Mastermix (Qiagen) vdi c|p moi (primer) ty thie't keco ma hi^u va trinh ty:
CaF30;5'-ACAGCCGACACATCCTCAGCC-3' CaR30: 5<X5AAGAATCGGGrGAGa?rGGC3.
Vdi ehu trinh nhi?t:
Buoc Bien tinh ban dau Bie'n tinh Bat d p moi Keo dai Hoan thien Giu mau
Nhi^t dQ C O
95 95 56 72 72 4
Tlim gian (giay)
120 25 25 40 300 Den l(hi
Sochuki 1 35
1 ay mau
DI TRUYEN - GIONG VAT NUOI San p h ^ n PCR dupe di?n di tren gel
agarose 1,5% va dupe tinh s^eh bang kit tinh sach san p h ^ PCR bang bg kit Gel Extraction Kit ciia hang Qiagen, Cac bude thue hien theo hudng d i n eiia nha san xuat.
Sii dung bg hoa chat BigDye terminator cycle sequence kit vS.l (Applied Biosystems, My) de danh daii huynh quang san phain PCR.
San ph&n eua phan iing danh dau huynh quang dupe tinh sach bang cpt sephadex G-50 cua hang GE Healthcare ^ y ) . Cae bude tiiue hien theo hudng dan ciia riha san xua't. Gai trinh ty duge thyc hi?n tren may dpe trinh ty ABI 3100-Avant Genetic Analyzer.
Phan tich vd xit ly s6^U^u
So sanh, sap xe'p, phan nhom eae trinh ty DNA giai ma duge thye hipn bang phan mem BioEdit (Hall, 1999) va DnaSP (Librado va Rozas, 2009). Xae dinh khoang each di truyen va xay dung eay phat sinh chiing loai tren phMi mem MEGA 6.0.5 (Tamura va ctv, 2013).
3. KET QUA VA THAO LUAN 3.1. D | c di^m di truyen mgt so giong cho
Gio'ng ehd H'mong ege duoi va Vi?t Dingo la hai gio'ng ehd cd nhieu vj tri nucleotide bie'n doi nha't vdi 10 vi tri, nhung xet tren tong so' 120 mau phan tich eiia gio'ng H'mong epe duoi vdi 20 mau ciia gio'ng Vi?t Dingo thi giong Viet Dingo eo ty I^ bie'n doi to't hon. Con thap nha't la hai giong Akita va Dingo Ian ehi vdi 6 vi tri ed xua't hi?n sy thay doi cae nueleotitde(nu). Tie'p theo do la 3 gio'ng H'mong dai duoi, Sharki, Sharpei ed 7 vi tri bie'n doi, con lai la H'Mong long dai, Bac Ha ciing eo 9 v; tri bie'n doi va LaiKa vdi 8 vj tri. Ke't qua thu dupe eiing chi ra r^ng cac bieh doi nay deu la bie'n doi dong hoan. Hon nua, vj tri thii 491 eo tdi 9/10 giohg luon xay ra sy thay the' giiia cae nucleotide va vung dupe phan tich kha da dang khi cac ke't qua deu eho thay cd su bieh doi.
Bing 2: Mpt so dac diem vung gen d-loop cua 10 giong cho Ten RioHR So Haplotype (Hap) Tong vi tri bien do'i Vj tri nucleotide xay ra bien doi H'Mong coc duoi
H'Mong dai duoi H'Mong long dai Bac Ha Viet Dingo Akita Sharki Sharpei Dingo Ion LaiKa
74,89, 92, 94,112,114,127,421,487,495 93,98,118,131,425,491, 499 87,91, 96,116,118,131,425,491, 499 96,116,118,131,307,378,425,491, 499 2, 23,93, 98,116,118,131,425,491, 499 98,118,131,425,491,499 78, 96,118,131, 425, 491,499 23,93,96,116,118,425,491 93,96,98,118,131,491 93, 96,118,131, 3Q7,378,425,491 Cac kieu da hinh (haplotype) giiia cae
giong chd ciing ed nhieu sy khac nhau. Gio'ng ehd H'mong ege duoi vdi 120 mau eho ra nhieu kieu da hinh nha't vdi 8 hap, Laika va Bac Ha cung ed nWeu kieu da hinh khi chi vdi 20 mau dupe dem phan tich ma ed tdi 7 hap. Thap nha't la giong Sharpei ehi eo 3 kieu da hhih.
Su da dang ve di truyen ciia cac gio'ng ehd la khac nhau nhung kha dong deu, cac vi tri xay ra sy thay doi tu nucleotide 74 de'n nucleotide 499 eho tha'y ph&i md dau trong vung gen d-loop la phan ed nhieu sy bie'n doi
nhat. Dieu nay eiing phu hop vi day la phan thupc vimg bie'n doi trong gen d-loop, hai vung eon lai la vung lap va vung bao thu sy bie'n doi hau nhu khong xua't hien (Kim va etv, 1998).
3.2. Khoang each di truyen giua cac gjffng cho Ma tran khoang each di truyen va cay phat sinh chiing loai ciia 10 gio'ng ehd dupe kiem tra eae gia thuye't va thii mo hinh tieh hoa bang chuong trinh phan mem MEGA 6.05 (Tamura va etv, 2013), cho tha'y mo hinh thay the' nucleotide phii hpp nha't la T92+G,
KHKT Chan nuoi So 10-2015 17
Tamura ba tham so' (Tamura 3-parameter) vdi cae ehi so' (Bayesian Information Criterion) BIC = 3196,189, -InL = 1018,189, Base frequencies: freqA = 0,291, freqC = 0,209, freqG=0,209, freqT = 0,291, Substitution model: Ti/tv ratio = 62,46) (Hasegawa va etv, 1985). Cac phep Idem tra cung xae nhgn hai mo hinh khae phii hpp cho
phan tieh dii li?u dau vao trong nghien cuu nay la K2 Kimura hai tham so' (Kimura 2- parameter) vdi cac dii so' (Bayesian Information Criterion BIC = 3202,444, -InL = 1026,714, Base frequencies: freqA = 0,250, freqC = 0,250, freqG = 0,250, freqT = 0,250, transition/transversion R = Ti/tv ratio = 71,39).
IBHl [AKl [LK]
|SP|
ISK]
(VD]
[HDI IHLl IHCl [DLl
BH 0,005 0,005 0,006 0,005 0,007 0,006 0,006 0,006 0,005
B i n g AK
0,005 0,006 0,005 0,006 0,005 0,005 0,005 0,005
3: K h o d n g each di tru;
LK
0,006 0,005 0,006 0,006 0,006 0,005 0,005
SP
0,006 0,007 0,006 0,007 0,006 0,006
SK
0,006 0,005 0,006 0,005 0,005
^n giiJa VD
0,006 0,007 0,006 0,006
10 g i o n g c h 6 HD
0,006 0,005 0,005
HL
0,006 0,006
HC DL
0,005 Khoang each di truyen giua cae giong
chd kha dong nha't, tii 0,5% deh 0,7%, eho thay moi quan h? di truyen cua eac giong ehd nghien euu la kha gan gui.Trong nghien cuu cua Tsuda va ctv (1997) thi khoang each di truyen giQa cac gio'ng chd tir 0%'deh 3,19%.
Nhu v^y khoang each di truyen giira cac giong cho thu duge d Ha Giang va Lao Cai tha'p so vdi cac gio'ng eho khae tren the'gioi.
3.3. Moi quan h$, cay phat sinh chiing lo^i ciia cac giong cho
3.3.1. Xdc djnh cdc nhom Hap cua 10 giohg cho Da xac djnh dupe 20 nhdm Hap ed hinh ty giong nhau hoan toan trong tong so'ciia 55
Hap thupe 10 giong ehd nghien dm. Nhdm it nha't cd 1 Hap ciia 1 giohg gom eae nhdm Hap: 9, 13, 14, 15, 16, 18, 19, 20. Dae bi?t, nhdm Hap 7 chua 9 Hap ciia 9 giong chd:
H'Mong epe duoi, H'Mong dai duoi, H'Mong Long dai, B^e Ha, Vi?t Dingo, Akita, Sharki, Sharpei, Dingo, Lai Ka va nhdm Hap 3 ed 7 giong vdi cac Hap la BH.Hap3, LK.Hapl, SP.Hap3, SK.Hap3, HL.Hap2, HCHapl, DL.Hap3.
Nhu v$y, cae Hap nay khong d|ie trung cho giong ehd bmi dja nao va phan anh eac giong chd nay van giao phol vdi rdiau, tao nen sy dan xen cae Hap trong eae giong cho.
Nhdm Haplotype
Bing 4: Phan Io?i cac nhdm Haplotype cua 10 giong chd Ky hi?u Haplotype BH.Hapl, LK.Hap6
BH.Hap2, HL.Hapl, HC.Hap6 BH.Hap3, LK.Hapl, SP.Hap3, SK.Hap3, BH.Hap4, LK.Hap2
BH.HapS, AK.Hap2, LK.Hap3, SK.Hapl, BH.Hap6, AK.Hap3, LK.Hap7, SK.Hap2.
HL.Hap2, HCHapl, DL.Hap3 , HCHapS
, VD.Hapl, HD.HapS, HL.HapS, HC.Hap7, DL.Hap2
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Haplotype 7 8 9 10 11 12 13 14 15 16 17 18 19 20
Ky fii^u Haplotype
BH.Hap7, AK.Hapl, SK.Hap5, VD.HapS, HD.Hap4, HL.Hap3, HC.Hap3 LK.Hap4, HD.Hap6
LK.Hap5
SP.Hapl, HC,Hap2, DL.HapS SP.Hap2, VD.Hap7 SK.Hap4, HCHapS VD.Hap3 VDHap4 VDHap6 HDHapl
HDHap7, HCHap4, DLHapl HLHap4
HLHap6 DLHap4
332. Cdy phdt sinh diiing loai cua cdc giong cho Su dyng 55 Hap eiia eiia 10 gid'ng chd da xay dyng dupe eay phat sinh chiing loai cua chiing Iheo 3 phuong phap ME, MP, ML.
Ca ba cay phat sinh chiing loai ciia eae Hap thupe 10 gio'ng chd ban dia ed d ^ g hmh hpc tuang doi giong nhau, tru cay ME ed chiit khae bipt la BH.Hapl va LK.Hap2 tach thanh mpt nhanh, nhung cd gia tri bootrap tha'p (42%), phan anh moi quan he di tuyen giira cae Hap la cd dg tin cay tha'p. Nhin chung, eay phat sinh ehiing loai ed 3 nhanh ehinh:
Nhanh 1 gom nhdm Hap 6 ed 9 Hap ciia 9 giohg chd ed ky hipu BH. Hap6, AK.Hap3, LK.Hap7, SK.Hap2, VD.Hapl, HD.Hap5, HL.HapS, HC.Hap7, DL.Hap2
Nhanh 2 ehi cd 01 Hap thuge nhdm Hap 15 cd ky hi?u VD. Hap6. Nhanh nay ed chieu dai nhanh dai nhai, tdc cd mue tie'n hda xa nha't.
Nhanh 3 gom 16 nhom Hap cdn lai. Nhanh nay tiep tyc phan thanh eac nhanh nhd tuy nhien sy phan nhanh nay khong ro rang do gia tri bootrap 6 cac nhanh nay nhd hon 50% d ca ba phuong phap ME, ML, MP.
Ket hpp bang doi chieii trinh tu Hap va cay phat sinh chung loai eho tha'y khdng ed su tach biet di truyen giira cac giohg ehd ban dia
ciia Ha Giang va Lao Cai. Su chia se cac Hap trong 10 giong chd va sy phan hda khong ro rang tren eay phat sinh chung lo^i cho thay 10 giohg chd d Ha Giang va Lao Cai cd quan hp gan gui, thudng xuyen ed su giao phoi Ian nhau. Dieu nay cd tiie la do day la dja ban giap danh nhau nen viee eac ca the cho qua l?i giao phol ciing nhu tap tinh ciia ngudi dan ban dia thudng mang ehd di theo vy miia, di chg nen ke't qua ve moi quan hp di truyen eac gio'ng ehd d day kha gan giii la dieu de hie'u.
De dam bao va dinh hudng bao ton nguon gen eho timg giong ehd, tranh sy pha t?p eae giong vdi nhau thi cong quan ly, nhan nuoi va eham sdc dinh hudng can dupe chii trpng quan tam han niia.
4. K^T LUAN
Viing phan tieh cd sy bie'n doi d eae vj tri nucleotide d?c bi$t la d nu491, trong dd gio'ng H'mong epe duoi va Vi^t dingo cd nhieu vj tri bie'n doi nha't, eon gio'ng Akita la it nha't. Kieu da hinh ciing ra't da dang giira cac gio'ng.
Khoang each di truyen giira eae gio'ng chd kha dong nha't tir 0,5% de'n 0,7%. cho tha'y moi quan he di truyen kha gan gui.
Ca ba eay phat sinh chung loai cua eae Hap thugc 10 gid'ng ehd ban dja d Ha Giang
KHKT Chan nuoi So 10-2015 19
va Lao Cai CO ban la giong nhau, phan anh dja nao chiing 16 co su giao phoi voi nhau, cac Hap khong dac trung cho giong cho ban t?o nen sif dan xen cac Hap giua cac gicTng.
Hinh 1: Cay phat sinh chung lo^i Minimum Evolution (So 6 goc cac nh^nh la gi^ tri bootstrap)
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Hinh 2; Cay phat siiUi chung loai Maximum Parsimony (So d goc cac nhanh la gii tr| bootstrap
KHKT Chan nuaiseflO-2015
Hinh 3: Cay phat sinh chihng lo,i Maximum Likelihood v6i gia tri lnL(iog likelihood) = -1018,1 (So 6 goc cac nhSnh la gia tri bootstrap)
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TAI LI5U THAM KHAO
1 Clayton DA. (1991), RepHcalion and transcription of vertebrate mitochondrial DNA. Annu. Rev. Cell Biol,, 7:453-478.
2 Gyllensten U., Whaiton D., Josefeson A. and Willson A.C. (1991), Paternal inheritance of mitochondrial DNA in mice. Nature, 352:255-257.
3. Hall T.A. (1999), BioEdiL a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids. Symp. Ser., 41: 95-98.
4 Kim S., Lee S.E., Jeong H.W. and Ha J.H. (1998), The complete nucleotide sequence of the domestic dog (Cams fiimillaris) mitochondrial genome. Molecular Phylogenetics and Evoludon, 10: 210-220.
5 Librado P. and Rozas J. (2009), DnaSP v5: A software for comprehenave analysis of DNA polymorphism data Bioinformatics, 25:1451-1^
6 Bui Xuan Phmmg Dinh The Dung Tran Hihi Cai va Nguyen Tien Tung pOlO), Thanh phS;! khu hf cho nha tai mpt so tinh phia bac va b5c Trung bg Viet Nam, Tuyeh tap BCKH ve smh thai Nhiet doi, NXB Nong nghifp, Trang: 157-163.
7 Rebekah L. Gundiy, Marc W. Allard, Tamyra R.
Moretti, Rodney L. Honeycutt Mark R. Wilson, Keith L. Monson and David R. Foran (2007). Mitochondrial
DNA Analysb of the Domestic Dog: Control Region Variation Within and Among Breeds. Forensic Sci., 52(3): 562-572.
Stoneking M., Hedgecock D., Higuchi R.G., Vigilante L. and Eriich H.A. (1991), Populahon variation of hum an mtDNA control region sequences detected by enzymatic ampliScation and sequence-specific oligonucleotide probes. Am. J. Hum. Genet., 48:370-382.
Tamura K., Dudley J., Ne! M. and Kumar S. (2013), MEGA6.05: Molecular Evolutionary Genetics Analysis (MEGA) software version 6.0,5.
Tsuda K., Kikkawa Y., Yonekawa H. and Tanabe Y.
(1997), Extensive interbreeding occurred among multiple matriarchal ancestors during the domestication of dogs: evidence from inter- and intraspecies polymorphisms in the D-loop region of mitochondnai DNA between dogs and wolves. Genes Genet Syst.Aug, 72(4): 229-38.
Wallace D.C. (1986), Mitochondrial genes and disease.
Hosp, PracL, 21:77-92.
Wilson A.C, Cann R.L., Carr S.M., George M., Gyllensten U.B., Helmbychowski K.M., Higuchi R.J., Palumbi S.R., Prager E.M., Sage R.D. and Stoneking M. (1985), Mitochondrial DNA and two perspectives on evolutionary genetics. J. Biol., 26:375-400.
TANG KHOI Ll/ONG, DAY M O L U ' N G VA CHUYEN HOA THLfC AN CUA TO HQP LAI LQN DU'C C u 6 l GIl^A DUROC
VA LANDRACE Ctl^if
Tran Van Hdo^, Nguyen Huu Tink^', Pham Tat Thang\
Nguyen Van Hap^ vd Nguyen Qud'c Vu' Ngay n h ^ bai bao: 05/03/2015 - Ngay nh?n bai phan bi$n: 22/03/2015
Ngay bai bao diroc chap nhan dang: 30/03/2015 T 6 M TAT
Myc tieu nghien ciiu nay la xac dinh to hp'p lai lgn di^c cuoi to't nhai trong cac to hgp lai giiia 2 giong Duroc (D) va Landrace (L), tren co so khao sat rngt so'to hgp lai thuan nghidi va phan tich cac thanh phan di truy&i anh huong deh to'c dg tang khoi lirong (TKL), day m a lung luc 100 kg (DML) va chuyen hoa thiic an (CHTA). Cac d u li|u nang suat ca the da dugc thu t h i p 2011-2013 t ^ cac co so giohg l<?n Binh Thang, Dong A va Soc Trang tren tong so 244 ca the lai (DL, LD, DxDL, LxDL) va 1.468 ca the D va L. Ke't qua cho thay trong cac to hgp lai giua D va L thi to hgp lai DL cho ke't qua khao sat to't nha't vai sinh tnrdng dat 731,3 g; day ma lung 10,63 mm va chuyeh hoa thiic an 2,68 kg. Cac to hgp lai chua khao sat thyc nghi?m DxLD, LxLD, DLxDL deu co nang sua't d y doan khong cao hon so voi cac to hgp lai da dugc khao sat th\rc nghidm.
Tuf khoa: Due lai cum cung, yeu todi truyen, chSn doan nang suat, l&n
' PhSn vien Chan nuoi Nam bg
• Tic gia d^lien he: TS. Nguyin Hihi Tinh, Pho giam doc Phiin vi^n Chan nuoi Nam b$. Dja chi: Kp. Hiep Th^ng, Binh Th^ng, DT An, Binh Duong. Dign thogi: 0903.315059; Email: [email protected]
KHKT Chan nuai So 10-2015