UTNG DUNG CONG NGHE THONG TIN TRONG VIEC XAC
DjNH DIEM DANG QUANG CUA HAI HdP CHAT
THAI NGUYEN HUNG THU, DANG THI NGOC LAN Trirdng Dai hoc Dugfc Hd Npi
1. Dat van de
Diem dang quang la buac song ma tai do do hap thu rieng ciia hai hgp chat la bing nhau. Xac dinh dugc diem dang quang la ca sa de co the trien khai phuang phap do t>; le hap thu. Mat khac vai dung djch chi CO 2 thanh phan co the xac dinh ngay dugc tong n6ng do cua chiing bang each do mat do quang tai diem dang quang ciia dung dich. Chiing t6i cho rang day la mot trong nhiing diem nen dugc chgn uu tien trong do quang tai nhieu buac song [1].
Ve nguyen tac neu co dung dich cua rieng tiing chat cimg dugc pha chfnh xac a mot n6ng do nao do dem quet pho va xac dinh cac diem giao nhau tren ph6 CO the suy ra dugc diem dang quang cua chiing. Tuy nhien de co dugc cac dung dich hoan toan chi co rieng chat nghien ciiu da kho va viec de co cac dung dich CO cimg n6ng do chinh xac cua ca 2 dung dich cGng khong de dang nen viec xac dinh diem dang quang thuang phai do dam va kh6ng chinh xac.
Vi vay, chiing toi thiy cin sit dung iing dung ciia cong nghe thong tin de tim mot bien phap giiip cho viec xac dinh diem dang quang ciia hai hgp chat dugc thuan tien va dom gian han.
2. NguySn liSu, thiet bi va phirang phap nghien cuu 2.1. Nguyen lieu
Chat chuan Cafein (CA) la 6ng 200 mg ham lugng 99,63 % ciia Vien Kiem nghiem thu6c Tmng uang (SKS: 0108242) dugc pha thanh diy dung dich CO nong do 5; 10; 15 va 20 )ig/ml.
- Chat chuan Natri benzoat (NB) ciia Merck dugc pha thanh day dung dich co nong do la 7,5; 15; 22,5;
va 30 |ag/ml.
- Dung m6i pha cac dung dich chuan la nuac cat 2 lan
2.2. Thiet bi nghien ciiru
- May quang pho UV-1700 series ciia hang Shimadzu;
- C6c do thach anh be day 1 cm;
- Khoang buac song quet ph6: 200 - 400 ma.
2.3. Phuang phdp nghien cim
Sir (lung kha nang chuyin giao ciii lieu ph6 giira phan mem dieu khien may quang pho sang EXCEL - mot cong cu xit ly s6 lieu thong dimg kit hgp voi cac bien phap xii ly thong ke th6ng dimg dl suy ra diim dang quang.
3. Kit qua nghien cmi
De CO the tien hanh ngi suy diem dang quang chiing toi xac dinh ph6 hap thu nhiing dung dich chuan co nong do khac nhau cua cac chat nghien ciiu.
Day chuin vai tiing chat cin dugc pha sao cho co khoang n6ng do chung. Vai phan mem xit ly pho, thu dugc bang so lieu pho la m6i quan he giita buoc song va mat do quang nhu a hinh 1.
"•"! liflgj* : Bift 51 =lltaH].« MlgMdlam'
Hinh 1. Mpt giao dien cua so ket qud pho hdp thu vai bdng so lieu phd, pho hdp thu vd danh sdch
cdc phd CO the chon.
3.1. Saochepd^iei^h^^^^^^^^^^^
J i w i i g IBS*; ••^*l^ rt wl>-im^AIKl
riS
Hinh 2. Ddnh ddu chon bdng so lieu pho de co the sao chip sang phdn mem xic ly khde.
Vai su CO mat cua bang s6 lieu pho, nguoi dung CO thi danh diu nhu trong hinh 2 va sao chep sang EXCEL dl xu ly tilp.
Chuyin giao dii lieu giiia cac phin mIm tten may tfnh thu dugc bang s6 lieu lien quan giiia buac song va cac gia tri mat do quang cua timg dung dich khic nhau sau khi da quet pho nhu ttong hinh 3.
26 T^p chi KIEM NGHIEM THUOC - So 2.2011; Talp 9.(32)
;| " '
> - i
;
9
i
•: 1
;•:; -
l»ir iwo -TSVL.
C = . - - T , . *- -r - , - V . - - : i =
: : i ^ :;::;;
::::•; : E
iiili
"i:r
•iili
iff;
" i i ^ j
/J^
" U.-
0 u'-^
^' ?:;
: : ; : : ; : ; . : ; ; > ; : : ; : , : : : : : : • - ; '
!! "T:!!".'!!! 11'', !!"'-"'\!-',-''" !''"")
::S::;-t::i;-;:-_^- _.
^/i/i 5. DM- //ew ^Ma« /ze g/ira budc song vd cdc gid tri mat dp quang cua timg dung dich khac nhau sau
khi dd quet pho vd duac sao chep.
3.2. Xu ly die lieu de xdc dinh diim ddng quang NIU ttong bang s6 heu co 2 dung dich ciia 2 chit khao sat co nong do nhu nhau thi viec xac dinh dan gian nhat la bang d6 thi (hinh 4).
Co the xac dinh diem dang quang bing each khac la tinh hieu mat do quang ciia hai dung dich co nong do tuang duang. Cac n6ng do tuang duang cua mot ttong hai chat dugc tfiih ty le tii cac gia tii mat do quang ciia mot hay m6t s6 dung dich co n6ng do da biet.
~ L a i _ J |
i:2zi
Hinh 4. Xdc dinh diem cdt nhau ciia 2 pho hdp thu bdng do thi.
Vi du mat do quang ciia dung dich NB 15 dugc coi nhu la bing mot niia ciia dung dich NB 30 hay mat do quang ciia dung dich CA 15 dugc coi bang trung binh cong cua dung dich CA 10 va CA 20...
Hieu mat do quang ciia 2 dung dich co nong do tiiong duong se chuyen dau lan can diem dang quang.
Tiiy theo su chuyin diu co gan gia tri 0 hay khong ma quyet dinh chgn diim dang quang ngay tai buoc song do hay nim ttong khoang buac s6ng chuyen dau.
'~ii'~t^
J-^J-~J—1 1 • "
5 M..
( u»a
m un.i
1 .»..
u I M *
. . . • « i p . «
»a.fc=->»J " ^ ^ W f l l M I
f.
U I V M
O l l M
a r w
S L W O l t M
D I M l
am
I *
0X14 H O "
s
<114TI DUii OMt OHIO
kttd
l >
I)4W1 o j u r -
oMir lisnt
OJJM OJLW
0 2 * r
^ud
l a
U M W
0^
0 ) ' «
mai
IliJV,
^
0 1 ^ 1 '
.1 -i.J)
«ia.ir
o i m
""
as I2J<0 i ) - « i i i - i r
O A M a«a*.' IIJTX O J J ' i
^ * ™ M
|K«VJ I W I I I
u : f « i
atarn item omi
Cung CO the tinh toan mat do quang ciia cac dung dich tuang duang ciia ca 2 chat bang sir dung cac ducmg chuan tai cac buac song khao sat. Sir dung cac ham SLOPE va INTERCEPT dl xac dinh cac he s6 a va b cua duang chuan. Bang each sir dung cac kilu tham chieu 6 thich hgp cho phep sao chep cong thiic sang cac 6 khac mot each nhanh chong [2].
--^P* 1^ BW Pf* *7** ^ ^ * * " - ^ ~ ' * ^ * ^ W J l ^ ^ @ ^ ^ i f " ' ^ M I | | | B B ' ' W i*-tcr 'J Tt -
H •-- :ij f..fi.*«ii3 I..5V. :ia »» ffi.i 1 mmmmSmm t*rtr
u • i =!»'»• JiMW>y^-i:i>i=J.
D I E F :• H J K L 1 M 1 K 1 0 1 P tSUm B , I CA 1 NB
3 : 5
ioy-
5 01904 6:01866 j'OlZli S 01-83
10 0 4i30 IS HOU 0 295.';
0 2SS5 02KI9
15 0<8J5 0JO91 0 398"
0J882 0-3"3
20 ! - ' If ' 11.5 (|-4ti.< '•:.'• •• . V u'm ".Ji%
0 502- 0 4 B 1 04-49
0 2384 0 : 2 " i 02151
ii'm
orr
0456B 04331
K .Ti\E naa/.s moiimimmlim:/
HMdr
1 24-"6 0-491 0-14- 06S01 0644i it I mi lOOlE 09^^
09094 08621
CA « WUCK k k It IS
HTsni usas QOBSO. OCEG? nanai 422EI|
4)093^ 00217 OOBS* 0(D34 OODOI 4).0BtlI] CCGIQ 0IB30 Htm OCOS {lOBBEO ODZU 0(823 DGIO OIUE AaSEBD D0197 0013 00067 DOOE
w m^mm^mrii^
I H
^n 1
>\ • 1
.^nA <J. Xdc dinh hieu mat dp quang cua hai dung dich co nong dp tuang duang theo dudng
chudn vdi cdc he so a vd b.
Tiep theo tinh hieu mat do quang ciia 2 dung dich CO n6ng do can khao sat (vi du a hinh 6 tinh cho n6ng do ciia 2 chat cimg la 14) va xac dinh diem tuang duang tuang tu nhu tten sau khi da sao cong thiic sang cac 6 khac cung cot.
Co the kiem tra them vai mot so n6ng do khac bang sao c6ng thiic sang cac cot chi n6ng do khac (vi du ttong hinh 7 la sang n6ng do 13 va 15) hoac siia gia tri 14 bang cac gia tti khac. Cac buac xu ly ket qua tuong tu nhu tten.
^ -' , -'-,
K
"- ,,
WiTtkfcA
UAO U . ' l ) 23'.(
U'Q y - ' I J R O I i S _ * 21».0
i »-•
•> M* fBM H I
t f 01'"
a I ' M OIGM
orii 0 I ' « OI'CM U I M 6 u i e i s 01_<9«
ai.*«e
• . . i-\BM^a.i3m 11 OAIX OKU O ^ " . ' 010}
O i W ? O ' - J C 0 2 « E 0 i 6 0 1 OlfU 0 2i'6 OUZl
A 1»
O J U ) 0 « » l 0.WI7 Oiai 0 J " 3 OJfiiS"
C J " « O^A^0 0 i i " 6 O J W 6 SIM
2»
0-40<
05160 0<02'' OJSPl
or-»
0 4 i 0 t O-HT.i 0-IM7 0 4224 0 4109 04000
NB
••f 0 4|l<
0 2 4 * . 0 2 W * i > ' - | 0 ' I ' l 0 2UJ1 O I P U o m ; o i < r 2 0 1 " ! 0 1 4 M
I F M J '
11 J - " -
" J ' l ? n4.*Jl 14091 OJS'O U.I«OS UJ)-4 0 314- 0 29.V)
1 2 * ID ] ; j ' ( . Ip«'i2 (,-,01 I r . i l S ( i - | J - , n " ' t 6 i,,^l,r,|' ( j ' ^ m i'"44'. " i o i l WW 0 " " » O t j - f o.<o;i 04610 0 4M9
U H I ' (I'WM { P - P ! o s ' o - O i i 2 U O'SiU
• " • - • " ' • • - ' • - • «
J l l
CA « .
""
illl l
lOIM^OKF
DDiei 00779
0 W , « a M 4 ) I I Z . 4 ) Z Z I 42M7 J
imis oooK « i E n 4>ISK oofsi
i m p D[m4)iEB)4)DeD.(ioHa IIE7I ooDi .otao fiasx j>atB twa o n e «nz.ooi9t,«coTa
ii]]]3_,otnx M i i r * M w * M w
<ta acm< * i m • * » t t w OiOnO 0i}1£3 DD77T 0D1SI OOSf 073F Odia 0039) ]•
Hinh 5. Xdc dinh diim ddng quang bdng hieu mat dp quang cua hai dung dich co nong dp tuang duang.
ITmh 7. Kiem tra them vdi mptsd nong dp tuang duang khde dua theo dudng chudn vdi cdc he so a vd b.
D I xac dinh diem dang quang chinh xac han sau khi xac dinh dugc vimg buac song chiia diem dang quang can quet ph6 vai buac chuyen song min hon.
Vi du ttong truong hgp nay buac chuyen song se la 0,1 nm thay cho 0,5 nm va cac buac tiep theo dugc xu ly lap lai mot each tuang tu. Cu6i ciing la kiem tta va minh hga lai bang d6 thi.
3.3. Bdn ludn
Tap chi KIEM NGHlEM THUOC - So 2.2011; Talp 9.(32) 27
Nhu vay, dua vao cac pho chuan don chat va bang gia tri giua buac song va mat do quang, co the xac dinh dugc diim ding quang theo 2 each vai ket qua nhu nhau:
Dua vao d6 thy. diem ding quang la giao diem cua 2 ph6 chat khao sat co nong do nhu nhau.
Tfnh hieu mat do quang ciia hai dung dich co nong do tuang duang. Khi hieu mat do quang nay chuyen diu thi do la lan can diim ding quang. Mat do quang cua dung dich tuong duang co the dugc tfnh
true tilp theo cac gia tri do dugc hoac theo phuong trinh duong chuin.
4. Kit luan
Ket hgp sit dung phin mem dieu khien cua may do ph6 va phin mIm EXCEL cho kit qua xac dinh buoc song dang quang nhanh chong, tien Igi, chinh xac. Tir viec xac dinh dugc buoc song dang quang, co the sit dimg buac song nay de trien khai them cac phuong phap dinh lugng true tiep cac dung dich nhiiu tharih phin bang quang ph6.
Tai lieu tham khao
1. Nese Dogan.H (1996), Simultaneous spectrophotometric determination of caffeine and acetaminophen in tablets by absorbance ratio technique, Farmaco, p. 145 - 146.
2. Thai Nguyen Hung Thu (2005), Ifng dung tin hoc trong mpt sd cdng tdc duac, Truong Dai hgc Dugc HaNgi,ttangl68- 188.
SUMMARY
Isosbetic point is a point at which the specific absorbance of two compounds is equal. Combined using of controlled UV-VIS spectrometer's software and EXCEL software to determine the isosbetic is fast, convenient and accurate. Since then, this wave can be used to apply on other measures to quantify simultaneously and directly the solution that has many compositions by UV-VIS spectrum.
THONG TIN KHOA HOC - TIN TlfC
DANH MUC THU6C KHONG DAT CHAT LI/0NG DA Dl/OC B 6 Y TE XUT LY TCTTHANG 12 NAM 2010 DEN THANG 5 NAM 2011
NGUYEN SY DUOC
Vien Kiem nghiem thuoc Trung uang De tang cuang giam sat cac ca sa san xuat, kinh doanh ttong viec thuc hien thu h6i thu6c vi pham chat lugng, d6ng thai giiip cho cac dong nghiep co them th6ng tin ve nhung hoat chat va cac ten thuoc can Imi y ve chat lugng, chiing t6i tap hgp danh muc cac thu6c vi pham chat lugng tit thang 12/2010 den thang 5/2011 ma Bg Y te da ra van ban thong bao thuoc gia, thu6c bi thu hoi va nit so dang ky ciia thu6c: 23 thu6c bi nit so dang ky, 1 thuoc gia va 34 thu6c bi dinh chi va thu hoi.
Chi tiet ve danh muc thu6c xin xem 6 bang duoi day:
Danh muc thuoc bj rut so dang ky
STT
1
2 3 4
Ten tfiuSc
Pasirine
Qbivita plus Qblvlta GS Supradivlta
So dang Ify
VD-8655-09
VD-7521-09 VD-6253-08 VD-0058-06
Ten ca s& san xuat va dang ky
Cong ty Co phan Duoc pham Ha Tay
Cong ty Co phan Duoc pham Ha Tay
Cong ty Co phan Du/gc pham Ha Tay
Cong ty Co phan Du/gc phim Ha Tay
Dia chi cua ca sa
80 Quang Trung, Ha Dong, Ha Noi
80 Quang Trung, Ha Dong, Ha N6I 80 Quang Trung, Ha D6ng, Ha Npi 80 Quang Trung, Ha Dong, Hk Npi
Van ban xir ly
373A/QD-QLD ngay 10/11/2010
373A/QD-QLD ngay 10/11/2010
373A/QO-QLD ngay 10/11/2010
373A/QO-QLD ng^y 10/11/2010
Hinftttiu-cxirlf
Rut so dang ky vl C6ng ty da san xuat thu6c nang
mem khong dOng dja chi ghi trong h6 so dang k^.
Nhu' tren Nhu' tren Nhui tr§n
28 Tap chi KIEM NGHIfM THUOC - So 2.2011; TSp 9.(32)