Nguyin Thi Hfing Lifin va Dtg Tap chl KHOA HQC & C 6 N G NGHE 177(01): 191-196
X A C D I N H H O A T T I N H K H A N G K H U A N T l T D I C H C H l f i T
C U A C A Y S A I D A T Wedelia chinensis m e r r . T R 6 N G T A I H U Y E N L U C N A M , T I N H B A C G I A N G T R O N G D I E U K I E N IN VITRO
Nguyin Thj H i n g Lign^ Dinh Thj Thily', Nguyen Thj Van' Than Thj Phu'g-ng', Tran Thj Hong', Nguyen HO-u Q u a n ' ' 'Trirdng Dai hpc Sir phgm - DH Thdi Nguyen. 'Trudng Trung hoc pho thong Cdm Ly
TOM T A T
Nghien cuu hoat tinh khang khuin djch chilt cua cay Sai dat nhSm ehftng minh vai trfi efta cay trong dilu tri mgt sfi bSnh bang phuang phdp truyen thing. Kit qua cho thdy, dich chilt tft thdn vd Id cda cdy Sdi ddt cfi khd ning khang dugc cac lodi vi khudn Staphylococcus aureus, Seratia marcescens, Sarcina lulea, Escherichia coli trong dilu ki^n in vitro a cdc nfing do tu 2,0-4,0 g/ml.
Hogt tinh khdng khuan cao nhat doi vdi lodi S. lutea, tilp theo Id S. aureus, S. marcescens vd E coli. Hogt tinh khdng khuan tft djch chiet cua la va thdn cay Sai dat khfing chju tdc dfing bdl nhi§t dfi vd hogt tfnh duy tri tren 80% khi xft 1^ d 100°C, trong thdi gian 20 phut.
Tu' khda: Hogt tinh khdng khudn, S. lulea. E. coli, cdy Sdi ddt, S aureus, S. Macescens M O D A U
Cdy Sdi dat (Wedelia chinensis Merr.) la logi cay cfi mgc hoang d nhieu tinh trong ca nude.
Theo Dfing y, cay Sai dat cfi vi ngpt, hai chua, tinh mdt vi vgy cay cd rat nhieu cdng dung chfta benh nhu: Tieu dgc, giai ddc gan, chfta viem tdy ngoai da, mun nhgt, x o gan, sung vii, rom sdy, choc dau, dau mat, sot va ufing phong b i i n chung bgnh sdi.
Cay sai dat chua rat nhieu thdnh phan hoa hpc tot cho CO the. Dich ep ciia cay chiia tinh dau hoa tan 11,2%); hpp chat beo 29,7%;
phytosterol 3,75%; chlorophylle 3,75%;
caroten 1, 14%; nhya 44,95%p; ngoai ra con cd duong, tanin, saponin, cac chat cd pectin, mucin, lignin va cellulose.
6 Viet Nam, cay Sai dat dupc ngudi dan sir dpng nhu mpt thdi quen de tdm cho tre em vd ngudi ldn khi bj rom sdy. Nghien ciiu ciia Vu Dinh Hoang va Dfi Danh Giap (2007) [1] chi diing Igi d phan lap cac chat trong cay. Trong khi, nghien cuu v l hoat tinh sinh hgc cQa cdy Sai dat chua cd.
Trong bai bao nay, chiing tdi tien hanh nghien Clhi hoat tinh khang khudn ciia dich chiet t u cay Sdi dat (Wedelia chinensis merr.) trfing tai huyen Luc N a m , tinh Bac Giang.
Tei 01669 238303. Email: [email protected] vn
V A T LIEU V A P H U O N G PHAP Nguyin lieu vd mdi trudng
Cdy Sai ddt: Dugc trdng tgi xa Cdm Ly, huyen Lyc Nam, tinh Bdc Giang.
Vl khudn: S. aureus, S. lutea, E. coli va S. marcescens do Khoa Sinh hgc, Trudng Dgi hoc Su pham Thai Nguyen cung cap.
Moi tru&ng nuoi cdy: Mfii trudng LB co thanh phdn gdm cao ndm men 0,5%; NaCI l,0%i; pepton 1,0%. Mfii truong LB dgc bd sung 2,0%. thgch agar.
Phirangphap nghien ciru
Chudn bi dich chiet-. Than va la ciia cay Sai ddt dupc rfta sach, cfi khdi lupng Ian lugt Id 10 g, 15 g va 20 g d u g c nghien nat trong cdi sft, bd sung 5 ml nude cat, hda deu va Igc bfi can thu dugc dich chilt d cac ndng dg lan lupt la 2,0; 3,0 va 4,0 g/ml.
77i«- nghiim hogl tinh khdng khudn ttr dich chiet cua la vd than cdy Sdi ddt doi v&i vi khudn iheo cdc bu&c: (1) Chudn bj cac dTa petri chfta mfii trudng LB dgc. (2) Hiit 30 pi dich nufii mdi loai vi khuan trong mfii trudng L B Idng da d u g c hogt hda Ien dTa mdi truong LB dgc va trai deu tren mat thach cho den khi khd. (3) Diing khoan nut chai vd trimg co dudng kfnh 1 cm due 4 gieng tren dia thach va nhd IOO pi djch chiet tu than va la cay Sai 191
Nguyen Thj Hong LiSn vd Dtg Tap chi KHOA HQC & C6NG NGHE 177(01): 191 - 196 dat vao 3 gieng d cac nflng dp 2,0; 3,0 va 4,0
g/ml (gieng ddi chung bo sung nude). (4) Dgt cac dta petri da bo sung dich vao tii Ignh 4°C khodng 1-2 h cho dich chiet khuech tan deu vao mfii trudng va dat vao tu am nudi d SO^C, tu 18-24 h. (5) Do duong ki'nh vdng khang khudn (DKVKK), chup hinh va ghi Igi kit qua. Mfii thi nghiem lap Igi 3 lan. DKVKK dupe xac dinh theo cfing thuc: H = D-d (mm) Trong dd: D la dudng kinh vdng vo khudn tinh tu tam dye 16 (mm); d la duong kinh dye 16 thach (mm) [4],
KET QUA vA T H A O LUAN
Danh gia mau sac dich chiet tir cac bo phan ciia cay
Nhdm muc dich kiem tra kha nang khdng khuan tu cac bp phgn ciia cdy Sdi ddt de lua chgn bfi phan cua cay trong qud trinh nghien cftu, chiing tdi tdch rieng tftng bg phan than vd la de nghien rieng vd tien hanh thft nghiem. Ket qud cho thay, cac bfi phan khac nhau cua cay, dich chiet thu dupc cd mdu sdc khac nhau, djch chiet tft thdn co mau nau nhat, dich chiet tu Id co mdu xanh thdm.
Anh hirdng cua dich chiet tir than va la cay Sai d^t toi cac Ioai vi khuan trong dieu kien in vitro
Dich chiet tft than va la cay Sdi dat d nong dd 2,0; 3,0 vd 4,0 g/ml dupc tien hanh bfi sung vao mdi truoTig LB dgc de xac djnh hoat tinh khdng khudn vd tft dd lya chpn nfing dp thich hop dl xu Iy vi khuan trong dieu kign invitro.
Ket qua cho thay, mdi loai vi khudn chiu dnh huong cua djch chiet tii cac bg phan d nong do nghien ciiu la khac nhau.
Bang 1. DKVKK cda dich chiit lie thdn vd Id cdy Sdi ddt dot vdi S. aureus Mau
B K V K K it cac nong d$ (mm)2,0 g/ml 3,0 g/ml 4,0 g/ml
Thdn 27,3±1,5 28,7 0 9 29,0±2,0
Ld 28,3 ± 2,4 28,0 ± 2.7 28,7 ± 2,2 D6i vdi vi khuan S. aureus, DKVKK tu djch chiet cua than dgt 27,7 mm d n6ng do 2,0 g/ml; DKVKK tang ddn d nong dg 3,0 g/ml (dgt 28,7 mm) vd dgt cyc dgi 29 mm d nong
192do 4,0 g/ml. Trong khi, dich chiet tu la DKVKK ldn lupt Id 28,3; 28,0 va 28,7 mm tuong ung vdi cdc nong dp 2,0; 3,0 va 4,0 g/ml. Nhu vay, DKVKK tu djch chilt cda than vd Id cay Sai ddt d nflng dp 3,0 va 4,0 g/ml djch chiet tu than cd khd ndng khang vi khudn S. aureus tdt hon so vdi la; trong khi d ning dp 2,0 g/ml thi hoat tinh khang khudn kem han va nfing dd dich chiet tu than cay Sai dat phu hop dupc su dyng trong thi nghigm Id 4,0 g/ml (Bang 1 va Hinh I).
Vi khuan S. lutea thupc nhdm cau khuan gram duong, duoc tim thay tren da vd trong rugt gia cua ngudi. Vi khuan S. lutea gay nen miii tren ca the va khdng gay benh cho nguoi. Tuy nhien, khi hg mien djch suy giam, chung trd thdnh tac nhan gay benh ca hdi tren ngudi. Do dd, vigc nghien ciiu hogt tinh khang vi khuan nay tu dich chiet cua cay Sai dat cd y lighTa trong phdng ngua va han che mpt so tac hgi do vi khuan nay gen nen
A B Hinh L DKVKK lic dich chiit cua thdn (A) vd la
(B) cdy Sdi ddt dot vdi S. aureus trin dia petri Gl: Ndng do 2 g/ml. G2: 3 g/ml; G3: 4 g/ml Bang 2. DKVKK cua djch chiit tic thdn vd la cdy
cdy Sdt ddt ddi vdi S. lutea X BKVKK a cdc nong dg (mm)
2,0 g/ml 3.Q E/ml 4,0 g/ml Than 29,7 ± 0,4 30,0 ± 0,7 30,0 ± 1,3 Ld 29,0 :fc 0,7 30.7 ±1,1 31,0 ±0,7 .
A B
Hinh 2. DKVKK tic dich chiit cda thdn (A) vd Id
(B) cdy Sdi ddt ddi vdi S. lutea trin dia petri Gl: Ndng dd 2 g/ml, G2: 3 g/ml; G3: 4 g/ml
i l l
Nguyen Thj Hfing Lien vd Dig Tap chl KHOA HQC & CONG NGH$ 177(01): 191 -196 Ket qua xdc djnh hogt tinh khang vi khuan
S. lutea ciia dich chiet tft than vd Id cdy Sdi dat d cac ndng dp tir 2,0-4,0 g/ml cho thay:
DKVKK ciia dich chilt t u than dgt 29,7 mm d nong dp 2,0 g/ml va dgt 30 mm d n i n g dp 3,0-4,0 mg/ml. D K V K K khfing cd sy thay dfii nhilu d nfing dp tft 2,0-4,0 mg/ml. Trong khi, djch chiet tu Id cd kha ndng khang vi khuan S.
lutea Ion han djch chilt tu than, D K V K K tdng dan tu 29,7 mm d ndng do 2,0 g/ml den 30,7 mm d nflng dp 3,0 g/ml va dat manh nhdt d nfing do 4,0 g/ml ( D K V K K la 31 mm), Vi khuan S. marcescens cd the gay nen bgnh viem phfii, nhiem trung huylt, viem mang ndo, nhiem trung dudng tiet nieu, nhiem trung mat, Khao sat khd ndng khdng vi khuan S. marcescens cua dich chiet t u than va Id cay Sai dat gdp phan su dung ngudn nguyen lieu tft ty nhien, han che khd nang su dung khdng sinh, Ket qua cho thay dich chiet tft than va la cay Sai dat cd khd nang uc che vi khuan S. marcescens phdt triln. D K V K K tu djch chilt efta la vd than tdng dan theo ndng dp tft 2,0-4,0 mg/ml. T r o n g dd, djch chiet t u thdn cay cho hoat tinh ftc che vi khudn S. macescens mgnh hon djch chiet tft la d ciing nong dp, D K V K K mgnh nhat cua dich chilt tft la va than ldn lupt la 25,3 vd 25,7 d nong dg 4,0 g/ml (Bang 3 va Hinh 3).
Bdng 3. DKVKK cita djch chiit tic thdn vd Id cdy Sdi ddt ddi vdi S. macescens
PKVKK it cdc nong flg (mm) 2,Qg/mi 3,0 g/ml 4,0 g/ml Thdn 24,7 ± 1,1
Ld 23,7 ±0,4
25,3 ±0,9 25,7 ±1,6 25,0 ± 1,3 25,3 ± 1,6
A B Hlnh 3. DKVKK tic dich chiit cita thdn (A) vd id (B) cdy Sdi ddt dot vdi S. macescens trin dia petri
Gl: Ndng do 2 g/ml. G2: 3 g/ml; G3. 4 g/ml Doi vdi vi khuan E. coli. Ket qud bdng 4 va hinh 4 cho thdy, dich chiet tft than va la cdy
Sai dat deu cd kha ndng tie che vi khudn E. coli d ca 3 ndng dg khao sat va hoat tinh khang khuan tang dan theo ndng do. DKVKK t u dich chilt cua thdn cay ISn lugt dat 26,7;
27,0 va 27,3 d nfing do t u a n g ung Id 2,0; 3,0 va 4,0 g/ml; trong khi hogt tinh khang khudn cua dich chiet tii' la cay cao han so vdi tft than cay vd D K V K K ldn lugt la 2 7 ; 28 vd 28,7 mm d nong do t u a n g irng Id 2,0; 3,0 va 4,0 mg/ml.
Bang 4. DKVKK cua dich chiet tic thdn vd Id cdy Sdi ddt dot vdi E. coli
~77T BKVKK d-cac nong dd (mm)
^" 2,0 g/ml 3,0 g/ml 4,0 g/ml Thdn 26,7 ± 0,9
Ld 27,0 ±0,7
A B Hinh 4. DKVKK tic dich chiit cda thdn (A) vd Id (B) cdy Sdi ddt doi vdi E. coli trin dia petri Gl • Ndng do 2 g/ml, G2- 3 g/ml, G3 4 g/ml So sanh anh hudng ciia dich chiet tu' than cay Sai dat tdi sy phat trien ciia 4 lodi vi khuan S. aureus, S. lutea, S. macescens va E. coli a cung nong dp, ket qua bang 5 cho thay hoat tinh khang vi khuan gram duong S. aureus vd S. lutea manh hon rat nhieu so vdi vi khuan gram am 5*. macescens vd E. coli d ca 4 ndng do khdo sat, 6 nfing do 4,0 g/ml, hogt tinh khang 4 loai vi khuan S lutea, S. aureus, E. coli va S. macescens lan luot la 30,0; 29,0;
27,3 va 25,7 mm.
Bang 5. DKVKK cua dich chiit tic thdn cdy Sdi ddt ddi vdi 4 lodi vi khudn Lodi vi BKVKK is cac nong dg (mm) khu^n 2,0 g/ml 3,0 g/ml 4,0 g/ml S. aureus 27,3 ± 1,6 28,7 ±0,9 29,0 ±2,0 S. lulea 29,7±0,4 30,0±0,7 30,0± 1,3 S macescens 23,7 ±0,4 25,0 ± 1,3 25,3 ± 1,6
£, coli 27,0 ±0.7 28,0 ±0,7 28,7 ±0,9 So sanh anh huong ciia djch chilt tu la cay Sai ddt tdi sy phdt trien efta 4 lodi vi khuan S. aureus, S. lutea, S. macescens va E. coli a
m
Nguyin Thi Hflng Lien vd Dtg Tgp chi KHOA HQC & CONG NGHE 177(01): 191-196 cung ndng dp. Ket qua cho thay, djch chilt t u
Id cay Sdi dat ftc che vi khuan S. lulea manh nhdt dat 31,0 mm, tiep den vi khuan S. aureus (dat 28,7 mm), vi khuan E. coli (dgt 27,3 mm) va vi khudn S. macescens (25,7 mm) d ndng dp 4,0 g/ml (Bang 6).
Bang 6. DKVKK ciia djch chiit tic than cdy Sdi ddt ddi vdi 4 lodi vi khudn
BKVKK d- cdc nong do (mm) 2,Q g/ml 3,0 g/ml 4,0 g/ml Lodi vi
khuin
tft la Idn lupt dgt 28,3; 28,0 va 28,7 tuong ung vdi cac nong dp 2,0; 3,0 va 4,0 g/ml. N h u vay, djch chilt sau khi x u ly bdi nhiet tu la, hogt tinh khdng khudn giam 9-15% so vdi djch tuai, D I I vdi dich chilt t u thdn cua cay Sai ddt sau khi xft Iy nhiet hogt tinh khang vi khudn S. aureus ldn lugt dgt 24,3; 25,0 va 26,3 mm tuang ftng vdi cdc nfing dp 2,0; 3,0 va 4,0 g/ml. Trong khi, dich chilt tuoi tu than, hogt tinh khang khuan lan lupt dgt 27,3; 28,7 va 29,0 tuong ling vdi cac nfing dp 2,0; 3,0 va 4,0 g/ml. N h u vgy, dich chiet tft than sau khi xft ly bdi nhigt dp, hogt tinh khang khudn giam 9-13% so voi dich tuai (Hinh 5A).
Hoat tinh khang vi khuan 5'. lulea cua djch chilt t u Id sau khi x u Iy nhiet cua cay Sai ddt lan lupt dgt 25,0; 25,3 va 26,0 mm tuang ftng vdi cdc ndng do 2,0; 3,0 va 4,0 g/ml. Trong khi, hogt tinh khdng khuan cua dich chiet tuoi tft la lan lupt dgt 29,0; 30,7 va 31,0 tuang ftng vdi cac nfing dp 2,0; 3,0 va 4,0 g/ml. Nhu vay, djch chilt tft la sau khi x u ly bdi nhiet do, hogt tinh khang khuan giam I3-I6%i so vdi dich tuoi. Dfli vdi dich chiet tft thdn cua cay Sai dat sau khi xft ly nhigt boat tinh khang vl khuan S.
lutea Idn lucrt dgt 25,3; 26,7 va 27,0 mm tuang ung vdi cac ndng dp 2,0; 3,0 vd 4,0 g/ml.
Trong khi, djch chiet tuoi t u than, hogt tinh khang khudn ldn lupt dgt 29,7; 30,0 vd 30,0 tuong ftng vdi cdc ndng do 2,0; 3,0 va 4,0 g/ml. N h u vgy, dich chiet sau khi xft ly boi nhigt dp tft than, hoat tinh khang khudn gidm 10-15% so voi dich tuai (hinh 5B).
LiLlli
Hlnh 5. Biiu dd thi hiin hoat linh ice chi S aureus (A) vdS. lulea (B) tic dich chiil trudc vd sau khi xu ly vai nhiit dg cua Id vd thdn cdy Sdi ddt
DT-LA: Djch chiil tuat tic Id; XLN-LA: Dich chiit lie Id dugc xic ly nhiit;
DT-THAN: Dich chiit tuoi tic thdn, XLN-THAN: Djch chiit tic thdn dugc xic l;^ nhiit S aureus 28,3 ±2,4 28,0 ±2,7 28,7 ±2,2
S, lutea 29,0±0,7 30,7± 1,1 31,0±0,7 Smacescens 24,7 ± 1,1 2S.3±0,9 25,7±1,6 E. coh 26,7 ± 0.9 27,0 ± 0.7 27.3 ± 1.6 Anh hufirng ciia nhiet d o toi b o a t t i n h k h a n g k h u a n c u a dich chiet tur la v a t h a n cay Sai d a t t r o n g dieu kien invitro De xac dinh anh hudng cua nhigt dp tdi hoat tinh khang khuan trong dieu kien invitro cua djch chiet tu la va than cay Sai dat, chiing tfii da xft ly djch chiet nay d nhiet dfi 100°C trong thdi gian 20 phut, sau dd lay ra va thft nghiem. Dich chiet dupc xu ly nhiet va djch chiet tuoi dupc dem khdo sat hogt tinh khdng khuan, ket qua cho thay dich chiet dupc x u ly nhigt deu ftc che mgnh 4 loai vi khudn S. aureus, S. lutea, S. macescens va E. coli;
hoat tinh khang khuan tft dich chiet xft ly vdi nhiet thdp han so vdi tft djch chiet tuai, Hoat tinh khdng vi khuan S. aureus cua dich chiet sau khi x u ly nhigt tu la cua cay Sai dat lan lugt dat 24,0; 24,7 va 26,0 mm tuong \m%
vdi cdc nfing dp 2,0; 3,0 va 4,0 g/ml. Trong khi, hoat tinh khang khudn cua djch chilt tuoi
Nguyin Thj Hong LiSn vd Dig Tgp chf KHOA HQC & CONG NGHE 177(01): 191 - 196
Hinh 6. Biiu dd thi hien hoat linh uc chi E coli (A) vd S. macescens (B) tic dich chiil tru&c vd sau khi xic ly vdi nhiit do cda id vd thdn cdy Sdi ddt
DT-LA - Dich chiit tuai tic Id; XLN-LA: Dfch chiit lit Id dugc xir ly nhiit;
DT-THAN: Djch chiit tuai tU than, XLN-THAN: Dfch chiit tuthdn dugc xic ly nhiit
Hogt tinh khang vi khuan E. coli cua dich
chiet tu la ciia cdy Sdi ddt sau khi xu ly nhigt lan lugt dat 22,7; 23,3 va 23,7 mm tucmg ung vdi cac ndng dp 2,0; 3,0 va 4,0 g/ml. Trong khi, hogt tinh khang khudn ciia djch chilt tuoi tii la ldn lupt dgt 27,0; 28,0 va 28,7 tuong ung vdi cdc nflng do 2,0; 3,0 va 4,0 g/ml, Nhu vgy, dich chilt tu Id sau khi xu 1^ bdi nhigt dO, hogt tinh khang khuan gidm 16-17% so vdi dich tuoi. Dfli vdi dich chiet tft than cua cay Sai ddt sau khi xu ly nhiet hogt tinh khang vi khudn E. coli ldn lupt dgt 24,3; 24,7 va 25,0 mm tuong ung vdi cac nflng dp 2,0; 3,0 va 4,0 g/ml. Trong khi, djch chiet tuoi tft than, hoat tinh khang khudn ldn lupt dgt 26,7;
27,0 vd 27,3 tuong ung vdi cac nflng do 2,0;
3,0 vd 4,0 g/ml. Nhu vay, dich chilt tft than sau khi xft Iy bdi nhigt do, hogt ti'nh khang khuan giam 9,0% so vdi djch tuoi (Hinh 6A).
Khd ndng khang vi khuan S. marcescens cua djch chiet tft than va Id cdy Sai dat sau khi da xu ly nhiet dp cho thdy: Hogt tinh khang khudn ldn lupt dat 21,7; 22,0 va 23,3 mm tuong ung vdi cac nflng dfl 2,0; 3,0 va 4,0 g/ml bdi dich chiet tir la. Trong khi, hogt tinh khang khuan ciia djch chiet tuoi tft la lan lupt dat 23,7; 25,0 vd 25,3 tuong ung vdi cac ndng dp 2,0; 3,0 va 4,0 g/ml. Nhu vdy, djch chiet tu la sau khi xu ly bdi nhigt dp, hogt tinh khang khuan gidm 8-12%i so vdi dich tuai. Doi vdi dich chilt tu than cua cay Sai dat sau khi xu ly nhiet hogt tinh khang vi khuan Smacescens lan lupt dgt 21,0; 22,3 va 23,0 mm tucmg ung vdi cac nflng dp 2,0; 3,0 va 4,0 g/ml. Trong
khi, dich chiet tuoi tu than, hogt tinh khdng khudn ldn lupt dat 24,7; 25,3 va 25,7 tuong ung vdi cdc nong dp 2,0; 3,0 va 4,0 g/ml. Nhu vgy, dich chiet tu than sau khi xu Iy bdi nhigt dd, hogt tinh khang khuan giam mgnh hon tft 10-15% so vdi djch tu'oi (Hinh 6B).
Khi tien hdnh xft ly djch chiet tu than va Id ciia cay Sai dat vdi nhigt dp, cho thay hoat tinh khdng khuan ciia dich chiet khdng phai do protein qui djnh md Id do hgp chat thu cap cfi trong cay. Vi khi dun d 100°C, cac protein deu bj bat hoat va khfing the hign hogt tinh.
Trong khi, dich chilt xft Iy vdi nhigt dg vin cdn hogt tinh. Nhu vgy, budc dau cfi the nhan djnh hoat tinh khdng khuan cua djch chiet tu cay Sdi dat cd lien quan chgt che tdi mgt sd hgp chat thu cap cd hogt tinh sinh hpc nhu tannin, flavonoid; dgc biet la cac hpp chat phenol cd tinh chat phytonxide hay khdng sinh thyc vat. Ket qua budc dau ehftng minh dugc nhiet dp khfing dnh huong mgnh tdi hogt tinh khdng khudn cua djch chiet. Dieu nay ciing giai thich dupc vi sao, d mgt sfi dja phuong, ngudi dan thuong hay sft dyng cay Sai dat dun nude de tam cho tre nhd va ngudi Idn bj man ngua, rdm sdy. Qua trinh dun cay Sai dat trong nude la co sd de cac boat chat trong cay tiet ra ngoai va de chua cac bgnh rdm sdy, man ngfta d ngudi, giiip hgn che su dyng mgt so loai thufic khang sinh, Ket qua nghien cihi nay la ca sd ban dau cho vigc iing dyng la va than ciia cdy Sai dat trong viec chfta mgt sd bgnh lien quan den cac vi khuan gay bgnh d ngudi.
195
Nguyen Thj Hfing Lign vd Dig Tap chi KHOA HOC & CONG NGHE 177(01): 191 - 196 Nghien cftu hogt tinh khang khuan cua m d t s o
Ioai thyc vdt nhdn thdy, dich chiet tu cay Hg cfi khd nang u c che vi khudn S. aureus, E. coli a nfing dp 2 g/ml [ I ] , Tran M y Linh va c$ng sy (2013) [3] da chung minh dupc dich c h i l t tu mpt so thyc vat thupc rung nggp man cd kha nang ftc che mgt sfl loai vi khuan S.
aureus, E. coli d nfing do tu 10-100 mg/ml, DKVKK dgt tft 2-10 mm. N a m 2013, Le Thi H u a n g Ha va cgng s y [2] khi nghien cuu v l hogt tinh khdng khuan t u cii hanh tdm da chung minh dugc, cao chiet ciia cua hanh tam CO kha nang khang Igi mdt sd loai vi khuan, trong dd cd E. coli, S aureus va B. subtilis. N h u vay, so vdi mgt sd cdng bd trong nude, djch chilt tu cay Sai dat chiing tdi nghien cun cfi hogt tinh khdng khuan tot ban, ket qua nghien CUTI md ra tiem ndng ung dung cua cay Sdi dat trong y hpc.
K E T LUAN
Da chung minh d u p c dich chiet tu thdn vd Id cua cay Sai dat cd kha nang khang d u g c cdc lodi vi khuan gram am va gram duong:
S. aureus, S. marcescens, S. lutea, E. coli trong dieu kien invitro d cac ndng dp t u 2,0-4,0 g/ml. Hoat ti'nh khang khuan cao nhat doi vdi loai S. lutea, tiep theo Id S. aureus, S. marcescens va E. coli.
Da ehftng minh d u g c djch chiet tft Id vd than cdy Sdi dat ben vdi nhiet dp va hogt tinh khdng khuan tft la vd than cay Sai dat duy tri tren 80%. khi x u ly d 100°C, trong thdi gian 20 phut.
S U M M A R Y
IN VITRO A N T I M I C R O B I A L A C T I V I T Y O F C R U D E E X T R A C T S F R O M P L A N T WEDELIA CHINENSIS M E R R . EV L U C N A M D I S T R I C T , B A C GLVNG P R O V I N C E
Nguyen Thi Hong Lien^ Binh Thi Thuy', Nguyen Thi Van' Than Thi Phuong', Tran Thi Hong', Nguyen Huu Quan'*
'TNU- University of Education, 'High School of Cam Ly Study antimicrobial activity from the crude extracts of Wedelia chinensis tree to demonstrate the role of the plant in the treatment of diseases by ftaditionai methods. The results showed that the extracted liquid from the trunks and leaves of Wedelia chinensis are resistant to S. aureus, S.
marcescens, S lutea, E. coli in vitro at concentrations ranging from 2.0 - 4.0 grs/ml. The highest antimicrobial activity was found for S. lutea, followed by S. aureus, S. marcescens and £, coli.
The antimicrobial activity from Wedelia chinensis crude extracts of trunks and leaves is not affected by the temperature and the activity retains over 80% at lOCC for a period of 20 minutes.
Key words: Antimicrobial, E. coli, S. lutea, S. aureus. S. Macescens, Wedelia chinensis Ngdy nb0n bdi: 22/12/2017; Ngdy phdn bi$n: 08/01/2018; Ngdy duyft ddng: 30/01/2018
K i t qua nghien cftu b u d c ddu Iam can cu giai thich dugc thdi quen mgt so dja phuong ngudi dan s u dyng cay Sai dat dun sfii tam cho tre em vd ngudi Idn bi rdm say khdi benh, Ldi cdm ffn: N ^ i e n cuu nay d u g c sy hfi tro bdi de tdi KH&CN c i p Dai hpc Thdi Nguyen:
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Tei 01669 238303. Email: [email protected] w 196