Y Hpc TP. Hb Chi Minh * Tap 20 * Sb 6 * 2016 N g h i e n cmi Y hpc
TAC DUNG Dl/ PHONG R 6 I LOAN LIPID MAU
CUA CAO LA SEN - HA THU 6 DO (FOLIUM NELUMBINIS - RADIX POLYGONIMULTIFLORI) TREN T H U C NGHIEM
Huyrdi Thj My Hien*, Le Thi tan Phuong*, Nguyen Thi Son*, Nguyen Phuang Dung*
TOMTAT
Ma ddu: Roi loqn lipid mau Id nguyen nhan diinh gdy xo viia dong mqch (XVDM) va gay tdng ty li tie vong do benh ly tim mqch. Phong ngiea vd dieu tri rm loqn lipid mdu trd thanh moi quan tdm hdng dau. La Sen (LS) vd Hd thii 6 do (HTO) Id nhumg dugc lieu co sdn tiong nude vd dd dugc ket hgp sie dung tiin ldm sang dieu tri roi loqn Upid mdu. Tuy nhiin chua cd nghen cieu chieng mmh hiiu qud dieu hoa lipid mdu ciia viec kei hgp Id Sen vd Hd thu 6 do. De tdi ndy dugc thuc hien nham muc dich cung cap cdc bang chimg khoa hgc cho viec img dung kei hgp Id Sen vd Hd thii 6 dd tiong phdng ngiea roi loqn lipid mdu tien mo hinh thuc nghiim.
Mi^c tieu: Khdo sdt dgc tinh cap cua cao chiei nude Id Sen, cao chiei nude Hd thu 6. Xdc dinh tyli kit hgp cao Id Sen, cao Hd thii 6 do cd tdc dung du phong roi loqn lipid mdu. Ddnh gid tdc dung du phong rm loqn lipid mdu cua cao Id Sen - Hd thu 6 dd tien thuc nghiem.
Doi tugng vd phuong phdp nghien ciht: Chugt nhat tiang due vd cdi, chiing Swiss albino, tigng lugng 20
±2g, mua tqi Viin Pasteur TP. HCM. Nghien cieu thuc nghiem cd nhdm chirng tiin hai md hinh: roi loqn lipid mdu noi sinh (bang tiem phiic mo tyloxapol 500 mg/kg, 5%) vd rm loqn lipid mdu ngoqi sinh (bang u^g cholesterol 25 mg/kg).
Ket qud: Dmax ciia cao la Sen Id 11,818 g cao/kg chugt Lieu Dmax cua cao Hd thu 6 Id 6,09 g cao/kg chugt.
Tren mo hinh rm loqn lipid mdu ngi sinh, kei hop LS-HTO ty le 2-1 lieu LS 0,8 g - HTO 0,4 g gidm cholesterol todn phdn 23,21%, gidm 25,49% triglycerid, gidm LDL-C 43,43% nhung chua the hiin tdc dung lin HDL-C.
Tren mo hinh rm loqn lipid mdu ngoqi sinh, kei hgp LS-HTO ty le 2-1 lieu LS 0,8 g - HTO 0,4 gidm cholesterol todn phan 49,20%, gidm LDL-C 46,47%, tuy nhien chua thehien tdc dung tren TG vd HDL-C.
Ket luan: K^ hgp cao Id Sen - Hd thu d v&i tyli 2-1 co hiiu qud phong ngiea roi loqn lipid mdu toi han ty le 1-1 vd 1-2. Cao Id Sen -Hd thud dded tdc dung die phong roi loqn lipid mdu tien thuc nghiem.
TH Idioa: Ld sen, Hd thu o dd, du phdng roi loqn lipid mdu.
ABSTRACT
PREVENTING EFFECT OF THE EXTRACT COMBINED FROM FOUUM NELUMBINIS AND RADIX POLYGONI MULTIFLORI ON EXPERIMENTAL DYSUPIDEMLi MICE MODELS
Huynh Thi My Hien, Le Thi Lan Phuong, Nguyen Thi Son, Nguyen Phuong Dung
* Y Hoe TP. Ho Chi Minh * Vol. 20 - No 6 - 2016:11 -18 Background: Dyslipidemia is a major cause of atherosclerosis and increasing mortality due to cardiovascular disease. Prevention and tieatment of dyslipidemia has become an essential concern. Folium Nelumbinis (lotus leap and Radix Polygoni multiflora (Fo-fi root) are available domestic herb and have been combined in dyslipidemia tieatment However, no study has demonstiated preventing effect of Folium Nelumbinis and Radix Polygoni multiflori combination on experimental dyslipidemia models. This researdi is conducted to provide scientific evidences of Folium nelumbinis and Polygonum multiflorum combination in dyslipidemia tieatment.
* Khca Y Hoc Co Truyen
Tdc gid lien lac: ThS.BS. Huynh Thj My H i ^ DT 01682089687 Email [email protected] 11
N g h i e n c u u Y hpc Y Hpc TP. H b Chi M i n h * T a p 20 * Sb 6 * 2016
Aims of study: Survey the acute toxicity of aqu&yus Folium Nelumbinis extract and aqueous Radix
•oni multiflori extiact. Identify the combination doses (combined ratio) of Folium Nelumbinis and Radix Polygoni multiflori which regulates experimental dyslipidemia. Evaluate prevention effectiveness of Folium Nelumbinis and Radix Polygoni multiflori combined extiact in hyperlipidemic mice.
Methods: Male and female mice, Swiss albino stiain, weight 20 ±2 g, purchased at the Pasteur Institute, HCM dty. Experimental study was conducted vnth contiol group on two models: Endogenous dyslipidemia (by tyloxapol, i.p., 500 mg/kg, 5%) and exogenous dyslipidemia (by diolesterol 25 mg/kg, p.o.).
Results: Dma of Folium Nelumbinis extiact is 11.818 g/kg. Dnm of Polygonum multiflorum is 6.09 g/kg.
On the model of endogenous dyslipidemia. Folium Nelumbinis and Radix Polygoni multiflori combination (2-1 ratio) zoith dose 0.8 g Folium nelumbinis - 0.4 g Polygonum multiflorum decreased 23.21% total cholesterol, 25.49% triglyceride and 43.43% LDL-C. On the model of exogenous dyslipidemia. Folium Nelumbinis and Radix Polygoni multiflori combination (2-1 ratio) with dose 0.8 g Folium nelumbinis - 0.4 g Radix Polygoni multiflori reduced 49.20% total cholesterol and 46.67% LDL-C.
Conclusion: Combining Folium Nelumbinis and Radix Polygoni multiflori with 2-1 ratio is optimal for the prevention of dyslipidemia. Folium Nelumbinis and Radix Polygoni multiflori extiact has the preventive effect on experimental dyslipidemia models.
Key words: Folium Nelumbinis, Radix Polygoni multiflori, the preventive effect on experimental dyslipidemia models.
D A T V A N D E
Roi loan lipid mau la yeii to thuan loi gay nhieu benh ly tim mach do xo vira dong mach (XVDM). Theo thong ke, tjf le tir vong 6 benh nhan XVDM la 45%, trong do benh mach vanh chian 32% va tai bieh mach mau nao chian 18%'*'. Do do phong ngira, chan doan va dieu tri roi loan lipid mau tra thanh mot trong nhiing moi quan tam hang dau eiia cac nuoe. Den hien nay, rat nhieu thuoc dieu trj roi loan lipid mau duoc su dung, viec tim ki&n eae thuoc dieu tri roi loan lipid mau hieu qua, an toan va it tae dung phu tra thanh yeu cau het stic biic thiet
Nhfeu bai thuoc va vi ihuoc tCr thien nhien da duoc ehiing minh la co tac dung dieu hoa lipid mau nhu Nguu tat. Qua lau. Ha thii 6 do.
Ban ha, la Sen,... Trong do la Sen (LS) co tae dung chong oxy hoa, cai thien chiic nang thgn, khang viem, khang sinh, giiip giam can, dieu tri beo phi va dac biet la dieu tri roi loan lipid mau, nho vao CO ehe giam tong hcrp cholesterol, uc che hoat dong ciia men tong hop acid beo (FAS), aeetyl- CoA carboxylase va HMG-CoA reductase lam giam cholesterol toan phan (TC), triglycerid
(TG), lipoprotein ti trong thap (LDL-C) va tang lipoprotein ti trpng eao (HDL-C) trong mauW.
Ben canh do. Ha thii 6 (HTO) cung la mot trong nhiing thuoc duoc sii dting thong diing nhat trong YHCT, thong qua co che ire che tong hgp cholesterol, tang hoat dpng ciia lipoprotein lipase (LPL) va tang bat giu cac lipoprotein ti trpng thap, tang su bieu hien gen ciia thu the lipoprotein mat dp thap (LDLK) tu do lam giam nong do TC ro eung nhu lam giam LDL-C trong mau. Trong thuc te Iam sang, nhieu thay Ihuoc da ket hop la Sen va Ha thii 6 trong dieu tri rcS loan hpid mau, tuy nhien chua co nghien cihi chiing minh h i ^ qua dieu hoa lipid mau ciia viee ket hpp 2 dugc lieu tren. De tai nay dugc thue hien nham tim hieu tae dung dieu hoa lipid mau khi phoi hpp Ia Sen va Ha thu 6 do tren mo hinh thue nghiem.
DdlTlTONG-PHLfONGFHAFNGHltNCUtJ N g u y e n lieu n g h i e n cuu
Cac dupe lieu la Sen (Folium Nelumbinis) va Ha thti 6 do (Radix Polygoni multiflori) cung cap boi eiia hang dupe lieu Du Hung, quan 5, TP.HCM. Duac tieu dat tieu ehuah co so.
Y Hpc TP. Hb Chi M i n h * Tap 20 * So 6 * 2016 Nghien cihi Y hpc
Cao la Sen (LS), eao Ha thti 6 do (HTO) dupe bao ehe bang each sac 2 Ian voi nuac, loc dich chiet dem co de dupe eao. Cao la Sen co hieu suat chiet 16,34%, dp am 12,94%, dp tro 5,31%.
Cao Ha thii 6 eo hieu suat chiet 16,99%, dp am 15,54%, dp tro 4,15%. Dinh tinh bang sac k^ lop mong ciia eao la Sen, cao Ha thu 6 cho cac vet co mau sac va Rf tuong tu vod mau doi chieu la Sen, Ha thii 6.
Phuong tien Hoa chat
Cholesterol - Sigma, tyloxapol (triton WR- 1339) - Sigma, choKc add - Sigma.
Thuoc dm chieii
Atorvastatin 10 mg 16 F922- Cong ty Hamingo Ltd
Thiei bi dung cu
Can phan tich (do nhay 0,0001 mg), 16 nung, bep each thiiy, may chup hinh, mpt so dyng cii thuong quy trong phong thi nghiem.
D&ng vdt nghien ciiu
Chupt nhat trang Swiss albino, khoe manh, dong deu ve gidi tinh, 6-8 tuan tuoi, trpng lupng 20 + 2 g, cung cap bcri Vien Pasteur TP. Ho Chi Minh. Trong suot qua trinh thtjr nghiem, chupt dupe nuoi trong moi truong on dinh ve dinh duong.
Phuong phap nghien cuu
Khao sat dpc tfnh cap ciia cao la Sen, cao Ha thii 6 d6.
Giai doan tham do (4 chupt/16): Kh6i c^u tli Heu eao nhat co the berm dupe qua kim dau tu cho uong. Xac dinh lieu LDo (Ueu toi da khong gay chet) va Ueu LDioa (lieu toi thieu gay chet 100%).
Giai d o ^ xae dinh (20 ehupt /lo): Chupt duoc chia 16 va eho sir diing thuoc a cae Ueu trong khoang LDo va LDioo chia theo cap so nhan.
Danh gia ket qua
Xae dinh Dma. hoae LDso theo phuong phap Karber-Behrens (nai co).
Xae dinh ty Ie ket hpp ciia eao la Sen, cao Ha thii 6 do CO tae dung du phong roi loan Upid mau tren mo hinh roi loan Upid mau npi sinh bang tyloxapol (Triton WR -1339).
Chupt thii nghiem sau khi nuoi on dinh 1 tuan dupe ehia ngau nhien thanh 816 (moi 16 6 chupt).
Lo chiing, 16 benh: uong nuoe cat.
Lo atorvastatin (16 doi chieu): Uong atorvastatin 10 mg/kg.
L6 la Sen (LS): Uong la Sen vol lieu 1,2 g dupe Ueu/kg.
L6 Ha thii 6 do (HTO): Uong Ha tfiii 6 do vod Ueu 1,2 g duoe Ueu/kg.
L6 LS-HTO (1-1): Uong la Sen 0,6 g dupe Ueu/kg va Ha thii 6 do 0,6 g duac Ueu/kg.
Lo LS-HTO (1-2): Uong la Sen Ueu 0,4 g dupe Ueu/kg va Ha thii 6 do lieu 0,8 g dupe Ueu/kg.
L6 LS-HTO (2-1): Uong la Sen Ueu 0,8 g dupe Ueu/kg va Ha thii 6 do U ^ 0,4 g dupe U^/kg.
Chupt trong cac 16 dugfc cho uong thuoc thti nghiem hang ngay trong 14 ngay. Vao ngay 14, mpt gio sau khi uong thuoc, chupt dupe gay roi loan Upid mau bang tiem phiie mo tyloxapol Ueu 500 mg/kg, 5%. Vao ngay 15, mpt gio sau khi uong thuoc va 24 gia sau tiem tyloxapol, tien hanh lay mau tim chupt dinh lupng nong dp cac chi so triglycerid, cholesterol toan phan, LDL-C, HDL-C bang phuong phap do quang.
Danh gia tae dung du phong roi loan Upid mau ciia eao ket hgp la Sen - Ha thii 6 tren mo hinh gay roi loan Upid mau bang tyloxapol (hitonWR-1339).
Chupt dupe chia thanh 516 (m6i 16 6 chupt).
L6 chiing, 16 benh: Uong nuoc cat.
L 6 atorvastatin (16 doi chieii): Uong atorvastatin 10 mg/kg.
L6 LS-HTO (1): Uong la Sen Ueu 0,8 g dupe Ueu/kg va Ha thii 6 do lieu 0,4 g dupe Ueu/kg.
L6 LS-HTO (2): Uong la Sen Ueu 0,4 g dupe Ueu /kg va Ha thii 6 do lieu 0,2 g dupe Ueu/kg.
Nghien cuu Y hpc Y Hpc TP. Ho Chi M i n h * Tap 20 * So 6 * 2016
Chupt trong cac 16 dupe cho uong thuoc thii nghiem hang ngay trong 14 ngay. Vao ngay 14, mpt gia sau khi uong thuoc, chupt dupe gay roi loan Upid mau bang tiem phtic m6 tyloxapol lieu 500 mg/kg, 5%. Vao ngay 15, mot gia sau khi uong thuoc va 24 gio sau tiem tyloxapol, tieh hanh lay mau tim ehupt dinh lupng nong dp eae chi so triglycerid, cholesterol toan phan, LDL-C, HDL-C bang phuang phap do quang.
Danh gia tae dung dir phong roi loan Upid mau ciia eao k ^ hgp la Sen - Ha thii 6 tien m6 hinh gay roi loan Upid mdu bang cholesterol
Chugt dupe ehia ngau nhien thanh 5 16 (m6i 16 6 chupt):
L6 chiing: Uong nuoc cat.
L6 berth: Uong cholesterol 25 mg/kg tiong dau thuc vat va nuoc cat.
Lo atorvastatin (doi chieu): Uong cholesterol 25 mg/kg tiong dau thuc vat va atorvastatin 10 mg/kg.
Lo LS-HTO (1): Uong cholesterol 25 mg/kg tiong dau thuc vat va cao La sen - Ha thii 6 voi lieu tuong duong 0,8 g dupe Ueu la Sen va 0,4 g dupe Ueu Ha thii 6/kg.
Lo LS-HTO (2): Uong cholesterol 25 mg/kg tiong dau thue vat va eao La sen - Ha thu 6 voi lieu tuong duang 0,4 g dupe Ueu la Sen va 0,2 g dupe Ueu Ha thii 6/kg.
Nuoc cat hoac dung dich cholesterol pha tiong dau thuc vat dupe cho uong vao 8-9 gio sang m6i ngay. Atorvastatin va eao thuoc dupe eho uong vao liic 3 - 4 gia chieu. Sau 8 hian thii nghiem, gay me va lay mau tim chupt de dinh luang cholesterol toan phan, triglycerid, HDL-C, LDL-C bang phuong phap do quang.
Fhuong phap xu* ly thong ke so lieu thi;rc nghiem
Cac dii U^u dupe trinh bay duoi dang Mean
± SEM. Viec xii ly thong ke tiong de tai nay dimg phan mem SPSS phien ban 16.0. Phuong phap thong ke dupe sii dung la phep kiem Student cho 2 day so Ueu dpc Igp. So sanh stx khac nhau giiia cac nhom co tac dung dieu tri roi loan Upid mau bang phep ki&n ANOVA mpt chieu, mpt yeii to. Su khac nhau dupe xem la co ^ nghia khi gia tri p < 0,05.
KET QUA VA BAN LUAN Dpc tinh cap
Lieu Dmax eua cao la Sen la 11,818 g cao/kg ehupt, tucmg duong 361,62 g dupe Ueu kh6/ngu6i 60 kg. Lieu Dn™< eiia cao Ha thii 6 do la 6,09 g cao/kg chupt tuong duong 179,22 g dupe Ueu kh6/nguai 60 kg.
Dma). ciia eao la Sen gap 40 lan so vai Ueu su dung tren nguai va Dma* ciia eao Ha thii 6 do gap 30 lan so voi Ueu sii dung tien ngucri.
Dieu nay eho phep nhan dinh rang eao la Sen va eao Ha thii 6 do sii dung an toan tr&n siic vat thu nghiem.
Trong thue te sii dung ket hpp la Sen va Ha thii 6 do Cling ehua ghi nhan cac tac dung bat loi tien benh nhan.
De tai chua co dieu kien khao sat tac dung phu ciia cao la Sen - Ha thii 6 do khi sii dung dai ngay. Vai mpt ehe pham phong ngu'a va dieu tri roi loan Upid mau thai gian dimg thuoc thuang keo dai. Vi the, can phai tiep tyc tien hanh nghien a i u dpc tinh ban truang dien eua cao la Sen - Ha thii 6 do de lam eo sa eho cae buoc trien khai lam sang sau nay.
Xac dinh ty le ket hpp eua cao la Sen, eao Ha thu 6 do CO tae dung du phong roi loan Upid mau tren m6 hinh roi loan Upid mau npi sinh bang tyloxapol (Triton WR-1339).
Y H o c TP. H o Chi M i n h * Tap 20 * So 6 * 2016 N g h i e n c u u Y hpc
Bdng 1. Nong do diolesterol todn pKdn, triglycerid tiong mdu chugt nhat sau tiim tyloxapol (TYL) lieu 500 mg/kg 5%.
L6 (n = 6) Chi>ng
B^nh Aton/astatin USen(LS) m M e (HTO)
LS-HTO (1-1) LS-HTO (1-2) LS-HTO (2-1)
Lilu thCp nghiem Nud^ d t + Nu^c mu6i sinh i^
NlTOC c i t * TYL (SOOmg) Atorvastatin (10 mg/ltg) + TYL (500 mg)
LS(1,2g) + TYL(500mg) HTO (1,2 g) +TYL (SOOmg) LS (0,6 g) - HTO (0,6 g) + TYL (500 mg) LS (0,4g) - HTO (0,8 g) t TYL (SOO mg) LS (0,8 g) - HTO (0.4 g) * TYL (500 mg)
CIlolesteralTP (mg/dl) 121,32 ±17,14 534,84 ±34,85***
415,81 ±38,07 ***»##
369,93 ±62,25 ***###
465,97 ±91,82 * - 412,35 ± 55,66 ***##
346,11 ±101.46 ***«#
410.71 ±103,61 ~ «
Triglycerid (mg/dl) 198,34 ±38,57 1255,32 ±48,33***
1176,31 ± 66,57 •**#
1160,42±57.60**«»
1152,09±107,35***
1259,06±91,12***
1083,87 ±183,18***
935.40 ± 222,93 ***««
Khde biet coy nghm thong 1^ so voi nhom doing (*: p < 0,05, '*• p < 0,01, "*. p < 0,002) Khde biet co y nghm Ihmg kesovdi nhdm benh (tt: p < 0,05, tttt: p< 0,01, ###: p < 0,001)
Nhan xet.
Lo LS-HTO (1-1) giam (2,29%) cholesterol toan phan co y nghia thong ke so voi nhom benh. L6 LS-HTO (1-2) lam giam (35,29%) nong dp cholesterol, eo y ngjiia thraig ke so voi nhom benh. Lo LS-HTO (1-1) va LS-HTO (1-2) khong anh hirong tri so triglycerid.
L6 LS-HTO (2-1) lam giam (23,21%) nong dp cholesterol va gjam (25,49%) nong dp triglycerid, CO y nghia thong ke so vod nhom benh.
Nhu vay voi ea 3 ty le ket hcrp LS-HTO (1-1), LS-HTO (1-2), LS-HTO (2-1) deu Iam giam nong dp cholesterol toan phan va chi eo 16 LS-HTO (2- 1) lam giam triglycerid co y nghia thong ke.
Bdng 1. Nong do HDL-C, LDL-C tiong mdu chudt nhat sau tiim tyloxapol (TYL) liiu 500 mg/kg, 5%.
L6(n = 6) Chijng
Benh Aton/astatin La Sen (LS) Ha thij 6 (HTO)
LS-HTO (1-1) LS-HTO (1-2) LS-HTO (2-1)
Lilu thi> nghiem /Uuoc c^f + HuiK mu6i sinh 1^
Nuac ck + TYL (500 mgj Aton/astatin (10 mg/kg) + TYL (500 mg)
LS(1,2g)*TYL(500mg) HTO (1,2 9) + TYL (500 mg) LS (0,6g)-HTO (0,6g}* TYL (SOOmg) LS (0,4 g)-HTO (0,8 g)* TYL (SOO mgi LS (0,agl-HTO (0,4g)* TYL (SOOmg)
HDL,C (mg/dl) 66,17±1S,92 92,55 ±19.74 • 117,73±35,34if 67,56 ±27,74 109,58 ±53,64 ag,62±33,70 98,02±50,27 115,46±4S,76*
LDL-C (mg/dl) 15,48 ±4,51 191.22 ±25.47 " • 62,82 ±29,40-mm 70,29 ±34,35 "mm 125,97 ±71,78"
70,91166,23 m 31,31 ±38,34m#
108,17 ±63.52~m Khde biit co y nglad thmg M so vai nhom dmng (*: p < 0,05, **. p < 0,01, ***: p < 0,001)
Khac biet coy nghm thohgkesovdi nhom bink (9: p < 0,05, ##: p< 0,01, #ff#: p < 0,002) Nhqn xet:
L6 benh eo nong dp HDL-C tang 39,87%, LDL-C tang 11,35 Ian so voi 16 ehiing. Dieu nay phii hop vod ca che gay roi loan Upid mau eiia tyloxapol, do la lie che men Upoprotein Upase lam lie che oxy hoa VLDL tir do lam tang nong dp eiia cac chi so HDL-C va LDL-C.
L6 LS-HTO (1-1) CO nong dp LDL-C giam (62,91%) va 16 LS-HTO (1-2) lam giam (83,63%) nong dp LDL-C eo y nglua thong ke so voi nhom benh. Hai 16 (1-1), (1-2) khong anh huong tri so HDL-C.
Lo LS-HTO (2-1) lam giam (43,43%) nong dp LDL-C CO y nghia fhong ke so voi nhom benh va tang HDL-C (24,75%) khac biet eo y nghia fhong ke so voi nhom ehiing.
Ket qua 6 Bang 2 cho thay ca 16 ket hpp LS- HTO (1-1), (1-2), (2-1) deu lam giam ham lupng LDL-C so vod nhom berth. Tuy nhien chi co 16 LS-HTO (2-1) tac dpng Iam tang HDL-C.
Nhu vay, so Ueu trinh bay trong Bang 1 va Bang 2 cho thay ty le ket hop LS-HTO (2-1) voi Ueu LS 0,8 g/kg va HTO 0,4 g/kg la toi u u khi the
N g h i e n cuu Y hpc Y Hpc TP. H o Chi M i n h * Tap 20 * So 6 * 2016
Danh gia tae dung d u phong roi loan Upid mau ciia cao ket hpp la Sen - Ha thii 6 tren m6 hinh gSy roi loan Upid mau bang tyloxapol (bitonWR-1339).
Bdng2. Nong do cholesterol todn phan, triglycerid tiong mdu chugt nhdt sau tiem tyloxapol (TYL) lieu 500 hien tae dung lam giam tri so cholesterol,
triglycerid, LDL-C va tang HDL-C 6 mo hinh gay roi loan Upid npi sinh.
mg/kg 5%.
Ld(n = 6) Chimg
B^h Atorvastatin LS-HTO (1) LS-HTO (2)
Li^u ttiip n g h i ^ NiraN; cat -«- Nirdc mu6i sinh \i
NiTOccat •^ TYL (500 mg) Atorvastatin (10 mg/kg) + TYL (500 mg) LS (0,8g) - HTO (0,4 g) -H TYL (500 mg) LS (0,4 g) - [ ^ 0 (0,2 g) -^ TYL (500 mg)
Cholesterol TP (mg/dl) 115,55 ±17,71 422.28 ±38.83"' 382,34 ±35,56'"
399.68 ±43.22"' 422.72 ±39.77"
Triglycerid (mg/dl) ia8.26±59.66 1212.17 ±34.05"' 1222.03 ±48.39"' 1164,09 ±13.39'"m
1172.27 ±16,07 "*»
Khac biet co y nghm thmg kesov&i nhom chimg (*: p < 0,05, **: p < 0,01, ***; p < 0,001) Khde biit co y nghm thmg keso vdi nhom benh (It- p < 0,05, ##' p< 0,02)
Nhan xet Lo LS-HTO (1) eo nong dp triglycerid thap Ca 316 uong thu&r atorvastatin, LS-HTO (1), ^^^^t tiong cac nhom dieu tii, giam (3,97%) so vdd LS-HTO (2) deu lam giam cholesterol toan pKan ^ ° ^ benh. L6 LS-HTO (2) lam giam (3,29%) nhung khong eo y nghia thong ke. no"S ^9 h^glyeerid so vod nhom benh.
Bdng 3. Nong do HDL-C, LDL-C tiong mdu chugt nhat sau tiem tyloxapol (TYL) lieu 500 mg/kg, 5 % . Ld(n = 6)
Chirng B^h Aton/astatin LS-HTO (1) LS-HTO (2)
U§u thi> n g l i i ^ N t i ^ cM + Nu-dc muoi sinh ly
Nirdc cat + TYL (500 mg) Atomastafin (10 mg/kg) + TYL (500 mg) LS (0.8g) - HTO (0,4 g) + TYL (500 mg) LS (0,4 g) - HTO (0,2 g) + TYL (500 mg)
HDL-C (mg/dl) 62.02 ±22.03 96.94±14,64"
94.56 ±5,85"
107.75 ±11,54"
108.86 ±18.59"
LDL-C (mg/dl) 15.88 ±9.41 82.91 ±29.66'"
43.38 ±29.45#
59.11 ±44,85' 79.41 ±37,41"
Khde biet co y nghm thmg fee so vai nhom dtung (*- p<0,05, nhom benh (tt:p< 0,05)
Nhan xet Lo LS-HTO (1) va LS-HTO (2) lam tang HDL-C eo y nghia fhong ke so vdd nhom ehiing.
Lo LS-HTO (1) va LS-HTO (2) lam giam nong dp LDL-C (3,97%, 3,29%) so voi 16 benh kh6ng CO y nghia thong ke.
So Ueu trinh bay a Bang 3 va Bang 4 eho thay Ueu ket hop LS 0,8 g/kg - HTO 0,4 g/kg
Bdng 4. Ndng do cholesterol todn pKdn, triglycerid tiong mdu chugt nhat a mo hinh gdy rm loqn lipid mdu ngoqi sinh bang cholesterol.
I < 0,01, ***• p < 0,001); Khde bietco^ nghia thong keso oai
va LS 0,4 g/kg - HTO 0,2 g/kg lam gidm triglycerid, tang HDL-C, giam LDL-C va khong tac dung tren cholesterol toan phan.
N h u vay eao ket h o p LS-HTO da the fiien tac dung d u phong roi loan Upid mau tuy tren mo hinh gay roi loan Upid mau bang tyloxapol.
Tuy nhien hieu lue tren cac th6ng so Upid mau con phii hop vao lieu sir dung.
L6(n = 6) Chi>ng
B^nh Aton/astatin LS-HTO (1) LS-HTO (2)
Lidu tliCr nghiem NmSrcrft NUOT; cit + Ctiolesterol (25 mg/kg) Atonrastatin (10 mg/kg) + Ctiolesterol (25 mg/kg) LS (0.8 g) - HTO (0.4 g) + Ctiolesterol (25 mg*g) LS (0.4 g) - HTO (0.2 g) + Ctiolesterol (25 mg/kg)
Cliolestetol TP (mg/dl) 122.14 ±23.74 265.58*62,93 — 161,78 ±111,35*
134,92 i21.76mt 190,13 ±33,30 "It
Triglycerid (mg/dO 132,98±19,70 133,99±22,61 103,24 ±55,36 114,39 ±6,50 140,01 ±21,85 Khde biit co y nghta thong keso voi nhom diung (*: p < 0,(JS, **: p < 0,01, ***• p < 0,001)
Khac biit coy nghta thing ke so vai nhom benh (ti: p < 0,05, tttt. p< 0,01, US: p < 0,002).
Y Hpc TP. Hb Chi Minh * Tap 20 * So 6 * 2016 Nghien cihi Y hpc
Danh gia tae dung du phong roi l o ^ Upid mau tnia eao ket hpp la Sen - Ha thu 6 tren mo hinh gay roi loan Upid mau bang cholesterol.
Nhan xet Nong dp cholesterol toan phian 6 nhom LS-HTO (1) fhap nhat tiong cac nhom dupe dieu tri va giam (49,20%) so vod nhom benh CO y nghia thong ke (p < 0,001). Nhom atorvastatin va nhom LS-HTO (2) lam giam nong dp cholesterol toan phan (39,09%, 28,41%) so vai Bdng 5. Nong do HDL-C vd LDL-C tiong mdu chugt nhdt cholesterol.
nhom benh co y nghia fhcaig ke so vod nhom benh (p< 0,05).
Nong dp triglycerid cua nhom atorvastatin va nhom LS-HTO (1) giam (22,95%, 14,63%) so vcd nhom benh kh6ng eo y nghia thong ke.
Nhom LS-HTO (2) khong anh huong len nong dp triglycerid so voi nhom benh.
d md hinh gay roi loqn lipid mdu ngoqi sinh bang
La(n = 6) Chung
Benti Aton/astatin LS-HTO (1) LS-HTO (2)
Ljgu thCp nghiem Nuwccat Nuoc cat + Cholesterol (25 mg/kg) Aton/astatin (10 mg/kg) + Cholesterol (25 mg/kg) LS (0.8 g) - HTO (0.4 g) -i- Cholesterol (25 mg/kg) LS (0.4 g) - HTO (0.2 g) t Cholesterol (25 mg*g)
HDL^ (mg/dl) 75,15 ±20,48 75,90±17,14 54,82±30,80 54.84 ±18.53 100,98 ±32,52
LDL-C (mg/dl) 17.71 ±14.24 165,56±62,42"' 8a,29±84,99*
57,20 ± 11.24 * - » 61.15 ±23.78 " » Khde biit co y nghm thmg ki so v&i nhom chieng (*: p < 0,05,
fOidc biet co y nghta thmg ke so vdi nhom benh f#. p < 0,05, tt Nhan xet M6 hinh kh6ng gay tac dpng len chuyen hoa HDL-C trong mau ciia chupt nhat trang trong thii nghi&n n&i khong gfii nhan dupe tae dung cua thuoc.
Nong dp LDL-C 6 nhom LS-HTO (1) va LS- HTO (2) giam (65,45%, 63,07%) so voi nhom benh co y nghia thong ke (p < 0,01).
So Ueu dupe trinh bay a bang 5 va bang 6 cho ttiay su ket hop LS-HTO (1) (LS 0,8 g - HTO 0,4 g) va LS-HTO (2) (LS 0,4 g - HTO 0,2 g) Iam giam nong dp cholesterol toan phan va LDL-C, khong tae dpng len nSng dp triglycerid va HDL- C trong m6 lunh gSy roi loan Upid mau ngoai sinh bang cholesterol. Tuy nhien, hieu lue va u u tien tren thong so Upid nao ccm phu thuoc vao lieu k ^ h p p dupe six dung.
Tu ket qua Ihuc nghiem tren m6 hinh gay roi loan Upid mau npi sinh bang tyloxapol va m6 hinh gay roi loan Upid mau ngoai sinh bang cholesterol, ehung toi nhan thay ket hpp la Sen va Ha thii 6 vcd t)- le 2-1 eo tac dung du phong roi loan Upid mau tren th\re nghiem. Cac Ueu la Sen ket hpp Ha thii 6 voi tj- le 2-1 se tac dpng khae nhau len cac thong so Upid mau.
''*:p< 0,02, ***:p< 0,002) it: p< 0,01)
Vod ket qua nghien cuu nay, chiing t6i hy vpng che pham hi la Sen ket hpp Ha thii 6 do eo tiem nang iing dung tren lam sang trong du phong roi loan Upid mau. Can eo nhiing nghien ciiu tiep fheo ve lieu tac dung toi uu, danh gja tac diing ciia thuoc khi sue dung dai ngay, lua chpn dang bao die thich hpp de tien Ipi hon cho nguoi dung.
KET L U ^
Xac dinh dupe Ueu Dmax ciia eao la Sen la 11,818 g cao/kg chupt, gap 40 Ian so vol lieu sii dung tren nguod. Lieu Dmax eiia cao Ha thii 6 do la 6,09 g cao/kg chupt, gap 30 ^ so vai U'eu sii dung tren nguoi. Dieu nay cho phep nhan dinh rang eao la Sen va eao Ha thii 6 dupe sii dung tucmg doi tin toan tren dong vat thii nghiem.
Tren mo hinh roi loan Upid mau npi sinh do tyloxapol (500 mg/kg, 5%), ket hcrp eao la Sen - Ha fliii 6 do t^ le 2-1 voi Ueu LS 0,8 g/kg - HTO 0,4 g/kg va LS 0,4 g/kg - HTO 0,2 g/kg Iam giam triglycerid, tang HDL-C, giam LDL-C va khong tac dung tr&i cholesterol toan phan.
Tren mo Wnh roi loan Upid mau ngoai sinh do cholesterol (25 mg/kg), ket hpp cao la Sen -
Nghien curu Y hoc Y Hoc TP. H o Chi M i n h * T^p 20 * So 6 * 2016
Ha ttiii 6 do ty le 2-1 vai Ueu LS 0,8 g/kg - HTO 0,4 g/kg va LS 0,4 g/kg - HTO 0,2 g/kg Iam giam nong dp cholesterol toan phan va LDL-C, kh6ng tac dpng len nong dp triglycerid va HDL-C.
Nhu vay, cao la Sen - Ha fhii 6 do ty le 2-1 eo the dupe sii dung voi muc dich du phong roi loan Upid mau.
TAI U t u THAM KHAO
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Ngdy nhan bdi bdo: 30/07/2016 Ngdy phdn biin nhan xet bdi bdo: 32/08/2026 Ngdy bdi bdo diegc dang: 25/11/2016