C H A N N U O I D O N G VAT VA CAC V A N D E K H A C
ANH H I / 6 N G CUA GIOI TINH DONG TE B A O CAY LEN Si/
PHAT TRIEN CUA PHOI LON BAN NHAN BAN V 6 TINH
Hui/nh Tlti Hudng', Nguyen Tin Nhung', Nguyen Hodng Thinh- vd NguySn Viet Linh' N g a y n h a n b a i b a o : 28/10/2019 - N g a y n h a n bai p h a n bien: 30/11/2019
N g a y b a i b a o d u o c c h a p n h a n d a n g : 08/12/2019 T O M TAT
Viet N a m rat da dang ve cac giong lon ban dia, tuy nhien hien nay d o viec nhan giong t u phat va quan Iy khong chat che nhieu giong lon b a n dia Viet Nam d a n g bi suy giam tram trpng ve so lupng, tham chi co nguy co tuy?t chung. Vi vay, viec xay d u n g mot he tho'ng bao ton da d a n g smh hoc toan dien la vo ciing cap bach va can thie't. N h a m m u c dich bao ton va p h a t h u y da dang sinh hpc cua cac giong lon ban dia Viet Nam, chung toi nghien cuu tao phoi lon Ban n h a n ban vo tinh tii' dong te'bao sinh d u o n g va t n i n g lon Landrace. Trong bao cao nay chung toi trinh bay ket qua ve anh h u o n g cua gioi tinh dong te'bao cay len s u phat tnen ciia phoi lpn Ban n h a n ban vo tinh.
Ke't qua cho tha'y ty le p h a n chia sau khi cay nhan, t^ le hinh thanh phoi nang/so t n i n g nuoi va ty Ie phoi thoat mang/so t r u n g nuoi giiia hai n h o m s u d u n g te'bao smh d u d n g t u Ion Ban due va lpn Ban cai khong co s u khac biet. Tuy nhien, so' t e b a o / p h o i n a n g ciia cac phoi n h a n ban tir dong te bao cai cao h o n so voi 6 cac phoi tir d o n g te'bao dire (45,1 te'bao so v6i 37,5 te'bao) N h u v$y co the sir dung d o n g te'bao lpn cai lam te'bao cho de cai thien chat lupng phoi lpn Ban n h a n ban vo ti'nh
Til khoa: Lgn Bdn, nhdn bdn vo tinh, te'bao smh duong lgn Bdn due, te'bao sinh dirong lgn Ban cdi.
ABSTRACT
Effect of donor cell sex o n t h e d e v e l o p m e n t of Ban p i g embryos b y somatic cell nuclear transfer indigenous pig breeds of Vietnam are very diverse, now because of spontaneous propagation and poor management, there is a serious decline in number of them. So, establishment a biodiversity conservation system is extremely urgent and necessary. The aim of this study is conservation of Ban pig. In this research, we create Ban pig embryos by cloning method b y using somatic cell line from Ban male a n d female a n d Landrace oocytes In this report, w e present the effect of donor cell sex on the development of Ban pig embryos. Tlie results showed that the cleavage rate, blastocyte rate, hatching rate between two groups were not significant difference However, the number of cells per blastocysts of group using Ban female cell lines was higher than that of group usmg Ban female cell lines (45.1 cells with 37.5 cells). It, therefore, is possible to use Ban female cell Ime as a donor cell to improve the quality of Ban pig embryos by somatic cell nuclear transfer.
Keywords. Ban pig, male, female, somatic cell nuclear transfer.
I.DATVANDE
Lon ban dia Viet Nam la nguon tai nguyen sinh hoc ra't quy gia voi nhieu tiem nang chua duoc khai thac va phat huy hieu qua. Tuy nhien, hien nay do viec nhan gio'ng tu phat va quan Iy khong chat che, cac gio'ng lon ban dia dang hi Iai tap nhieu, ma't dan di nhirng dac tinh quy. Vi vay, viec xay dung mpt he thong bao ton da dang sinh hpc toan dien la vo ciing cap bach va can thie't. Nhan ban vo tinh la mot ' Vien Han lam Khoa hpc va cong nghe Viet Nam - Hoc vien Nong nghiep Viet Nam
'Tac gia hen h?. TS. Nguyen Viet Lmh, Vien Cong nghe sinh hoc, Vien Han lam KH&CN Viet Nam, DT 0949492281;
Email [email protected]
trong nhiing phuang phap hiiu hieu nham bao ton nguon gen quy hie'm cua cac Ioai dpng vat dang co nguy co bi tuyet chimg Vai muc dich thanh lap he tho'ng bao ton va phat huy da dang sinh hpc ciia cac gio'ng lgn ban dia Viet Nam, chung toi chpn Ion Ban la doi tugng Iam mo hinh de tieh hanh nghien ciiu. Giong Ipn ngi Ban thuoc Idp dpng vat co vu, chiing phan bo 6 khap cac huyen vimg mii cao cua tinh Hoa Binh, hong do chii ye'u tap trung 6 Da Bac, Cao Phong, Lac San, Mai Chau, Tan Lac. Voi dac diem thich nghi khi hau vung mii cao, chiu dung kham kho, it khi bi benh va chat lugng thit chac, tham ngon nggt thi Ion Ban ciing la mot giong lgn quy ciia Viet Nam.
KHKT Chdn nuoi so 256 - thdng 4 nam 2020
Tren the gioi, nhan ban vo tinh tren lpn dupc thuc hien thanh cong tir nhiing nam 1989 trong nghien ciiu cita Prather va ct\', ong da sir dung te'bao hen nhan va te'bao ciia phoi de cay vao tning. Te'bao hen nhan la nhiing te'bao chua co mang nhan de ngan nhiem sac the vol cac cau tnic khac cua te'bao. Vao nam 2000, Polejaeva va ctv, da thanh cong trong viec tao phoi Ipn nhan ban khi sir dung te'bao cho la te'bao hat. Da tao ra dugc Ion con bang phuang phap nhan ban vo hnh (Boquest va ctv, 2002), nhung su khac nhau ve hieu qua tao phoi nhan ban vo tinh due va cai van chua dupc chiing minh ro rang. Kumore va ctv, 2008 cho bie't, hieu qua san xua't Ion nhan ban vo tinh sii dung nguyen hao spi thai nhi dugc thu tir thai nhi dire va thai rJii cai co su dao dgng trong pham vi 0,64-0,90%.
O Viet Nam, Biii Xuan Nguyen va ctv (2006) da tao phoi Ign Ban nhan ban vo tinh tir cac te'bao sinh du5ng thu tir mot con Ipn Ban trudng thanh. Ket qua cho tha'y ty Ie phan chia dat 81,2% va ty le phat tnen den giai doan phoi nang la 12,5%. Viec tao phoi sit dung trung va tinh triing dong lanh da dupc mpt so tac gia tieh hanh tren giong Ipn Ban, cac nghien cuu da dugc cong bd' nhu nang cao kha nang thu tinh o'ng nghiem sir dung tinh trimg Ion Ban ddng lanh (Nguyen Viet Linh va ctv, 2018), tao phdi til' te bao tning Ion Ban ddng lanh (Somfai va ctv 2019).
Cac nghien a i u ve gidi tinli ciia dong te' bao cay anh hudng len chat Iugng phdi cung da dupc tieh hanh, Tuy nhien nhung nghien ciiu hen ddi hrpng Ign Ban con nhieu han che, dac biet la he tho'ng tao phdi nhan ban vd tinh.
Nham muc dich bao tdn nguon gen ciia cac gid'ng lcm ban dia Viet Nam, tao hen de cho cac nghien cuu tie'p theo, chiing tdi tieh hanh nghien ciiu:"Anli hudng cita hai ddng te bao due va cai dugc phan lap tir manh tai Ign de'n Iiieu qua tao phdi nhan ban vd tinh tren Ipn Ban". Nghien ciiu nay ciia chting tdi la budc di tie'p theo hen chang dudng nghien ciiu va phat trien cdng nghe sinh hoc sinh san de bao ton va phat tnen cac gid'ng Ign ban dia Viet Nam.
2. VAT LIEU VA PHIMNG PHAP 2.1. Vat lieu
Hda chat sir dung trong thi nghiem dupc mua tii' hang Sigma-aldrich. Buong triing Ipn Landrace va te bao smh dudng Ipn Ban due va cai dugc thu tiJ manh tai. Thi nghiem duoc thu'c hien hr thang 3/2019 deh thang 11/2019.
2.2. Bo tri thi nghiem
2.2.1. Phuang phdp thu vd nuoi thanh thuc trung lon
Buong triing Ign Landrace dugc thu ba- ld md va chuyen ve phdng thi nghiem trong vdng 1 gid trong nudc mud'i sinli Iy d SS-S/^C.
Buong triing sau dd dugc nia bSng dung dich PBS cd chira them khang sinh. Dich nang hiing dugc hiit tir cac nang tning nam tren be mat buong h u n g cd kich thudc 3-6mm bSng dng tiem va bom vao cac o'ng falcon 15ml dat d 37''C. Phiic hpp tning - te bao can noan (COCs) dugc chpn va thu nhan dudi kinh hien vi soi ndi khi quan sat va thao tac vdi phan lang ciia dich vira thu dupc Cac te'bao tning duoc nudi trudng thanh trong mdi trudng Porcine Oocyte Medium (POM) cd bo sung lOmg/ml yeu to tang trudng bieu bi (EGF), 10 lU/ml eCG va 10 lU/ml hCG. IMm dibutyryl cAMP trong 22-24 gid dau tien oia IVM. Sau do cac te bao tning duoc chuyen vao mdi trudng khdng cd hormone va dibutyryl cAMP va nudi trong 22-24 gid tie'p theo, cac te' bao tning dupc nudi d dia 4 gie'ng chiia 500[JI mdi trudng nudi dugc bao phii bdi dau khoang va dupc dua vao hi nudi vd trimg chiia 5% CO, d 38,5"C, do am bao hda. Mdi gie'ng nudi khoang 50 te'bao tning,
2.2.2. Phuang phdp thu vd nuoi te'bao sinh dudnglan
Te' bao sinh dudng lon due va cai dugc
phan lap tir mie'ng tai Ian Ban khde manh,
khdng benh tat d giai doan 2 thang tuoi, sau dd
dugc rira sach 3 Ian vdi dung dich dem mud'i
phosphate (Phosphate Bufered Salina-PBS) va
van chuyen ve phong thi nghiem d 37"C. Tie'n
hanh rira Iai bang PBS va cdn, cao bd phan da
ngoai va Idng, tie'n hanh thu Idp bieu bi. CSt
Idp bieu bi thanh cac manh nhd 1-2 mm va
nudi trong mdi trudng Dulbecco's Modified
Eagle's (DMEM) cd bd sung 10% FBS. Sau 5
ngay nudi, te' bao bam kin dia se duoc ca'y
KHKT Chdn nuoi sd 256 - thdng 4 ndm 2020
CHAN NUOI DONG VAT VA CAC VAN DE KHAC chuyen va ddng lanh. O cac lan cay chuyen, te'
bao se dupc kiem tra td'c dd tang h u d n g thdng qua viec de'm so lugng te bao bang each sii dung buong de'm hong cau (hemocytometer) tai thdi diem trudc va sau khi cay chuyen.
Trudc khi chuyen nhan 3 ngay, te bao dugc giai ddng va nudi trong DMEM 10%. Te'bao su dung cho chuyen nhan te' bao sinh duang la nhiing te'bao d cac Ian cay chuyen tir 2-6.
2.2.3. Phuang phdp chuyen nhdn te'hdo sinh duong lan
Chuyen nhan te bao sinh dudng duac thuc hien tren he thd'ng vi phau thuat voi may Piezo theo Otoi va ctv (2016). Theo do, te bao triing sau khi nudi thanh thuc se tie'n hanh Ioai bd sach lap te bao hat (cumulus) trong men hyaluronidase 10%. Triing thanh thuc vdi the cue rd rang dupc loai bd the cue va nhan trong mdi trudng TCM 199+10% FBS bang he thd'ng kim vi phau thuat Te'bao sinh dudng lgn don le duoc ca'y vao trung tao phuc hop trung - te'bao. Phiic hpp nay dugc tie'n hanh xung dien vdi ddng dien 1,5kV/cm, lOOps hong mdi trudng Maimitol 0,28mM.
Phiic hpp tning - te' bao dupc nudi trong dia 4 gie'ng d mdi trudng PZM3 cd bd sung 20% BME Amino Acid Solution (Sigma), 10%
MEM Non-Essential Amino Acid Solution (Sigma). Sau 5 ngay nudi bo sung them 10%
FBS. De xac dinh trang thai va cha't luang, phdi d ngay thii 7 dugc rira 3 Ian qua PBS cd chiia Img/ml polyvinylpyrrolidone (PVP).
Co' dinh phdi hong gipt lOOpl dung dich paraformaldehyde 4% trong PBS, pH 7.4 hong 1 gid d nhiet dp phdng. Rira Iai phdi 3 Ian trong PBS/PVP
Thi nghiem 1: So sanh ke't qua phan lap va nuoi ca'y te' bao sinh dudng Ion Ban due va cai trong mdi trudng Dulbecco's Modified Eagle's (DMEM) cd bo sung 10% Fetal Bovine Serum(FBS) qua cac Ian ca'y chuyen.
Thi nghiem 2: Nudi thanh thuc hiing lon Landrace lam nguyen lieu cho nhan ban vd tinh.
Thi nghiem 3: So sanh chat luong phdi lan Ban nhan ban vd tinh su dung 2 ddng te bao sinh dudng dupc phan lap d tren. Thi nghiem dirge thuc hien vdi 5 Ian lap lai.
2.3. Xu ly so lieu
So Heu dupc trinh bay dudi dang trung binh {Mean)±SE. Cac sd' lieu sau khi chuyen sang dang arcsin dugc xir Iy bang cdng thii'c ANOVA va so sanh Tukey's. Cac so'lieu duac coi la cd y nghia thdng ke khi P<0,05.
3. KET QUAVA THAO LUAN
3.1. Phan lap va nuoi cay te bao sinh duong lpn Ban due va cai
Te'bao sau khi nudi ca'y so cap, dupc ca'y chuyen vdi mat dp 10^ te'bao/gie'ng trong dia 4 gie'ng. So'te'bao tang sinh dupc ghi nhan sau 4 Ian ca'y chuyen lien tie'p vdi 3 ngay nuoi ca'y.
Bang 1. Phan lap va nuoi cay te bao sinh duong lon Ban due va cai sau 3 ngay nuoi
So TB Ign dye So TB lgn c, 2 200 000+4.082 212 500±8 539 3 228 000±3.741 234,000±4,000 4 230 000±3,162 232.000±3.741 5 234.000+2.449 228-000±3,741 _6 230.000±3.162 226.QO0±2.449
Nhu vay, ke't qua d bang 1 cho thay td'c dp nhan len ciia ddng te' bao Ign Ban due so vdi ddng Ipn Ban cai la khdng cd su khac biet qua cac lan cay chuyen tir lan thii 2 de'n thu 6 (200.000 den 230.000 te'bao va 212.500 den 226.000 te'bao), td'c do phat tnen ciia 2 ddng te' bao nay la nhu nhau.
3.2. Nuoi thanh thuc trung lon Landrace lam te bao cho trong nhan ban vo tinh
Bang 2. Ke't qua nudi thanh thuc trung Ign Landrace lam te bao cho hong nhan ban vo tinh
S - t t u n So tnrng (%) So tning (%) So tning (%) fhi^nehiem ^ ^ ' '^°^'^ ^'^^ **''^" 8'^' ^"*"
tm ngniem ^ , ^ ^ j khac 200 19(8,6±0,5) 154 (76,3±0,6) 16 (8±1,4)
Tren thuc te, trimg thanh thuc cd nhiem s3c the d giai doan Metaphase II, cd the quan sat dugc the cyc thii nha't. Tir bang 2 cho ta tha'y ty Ie thanh thuc ciia tning lpn Landrace trong he thd'ng dat tren 70%, tuong duong vdi cac phdng thi nghiem cua cac nudc phat trien, dap img dii sd' lupng va chat Iugng ve nguon te'bao trimg lam te'bao nhan de phuc vu cho nghien ciiu tao phdi lgn Ban bang phuong phap nhan ban vo tinh.
KHKT Chdn nuoi so 256 - thdns 4 ndm 2020 83
3.3. C h a t l u o n g p h o i lcm B a n n h a n b a n v o t i n h tir 2 d d n g t e b a o s i n h d u a n g Igfn d u e v a cai B i n g 3. C h a t l u o n g p h o i Jon Ban n h a n b a n vd tinh t u 2 d o n g te b a o s i n h d u o n g l o n Ban d u e va cai
Giai tinh Lon Lcm due
So truTig thi nghiem
780 770
So tnmg (%) thanh thuc
580 (74,3±fl,8)
560 (73,3±3,7)
So tning nuoi sau cay nhan
{%) 445 (76,5±1,2)
434 (77,2+2,6)
So phoi phan chia (%)/so tning
338 (75,8±1,2)'
340 (78,6±2,0)^
So (%) phoi nang/s6 tning
50 (11,3±1,3)^
50 (ll,7±l,2y'
So (7o) phoi Thoat mang/so
tning nuoi 18 (4,l±fl,7)'
17 (3,9±0,6)-^
So tebao /phoi nang
(Ngay 7) 45,1+1,9'' 37,5±1,3'' Ghl chit: Trong cung cot. so'lieu vdi cdc chd cdi khde nhau Id khde nhau co y nghta tho'ng ke (P<0,05).
Thao tac NBVT Phoi NBVT tii dong TB cai Phoi NBVT tir dong TB due M p t s d h i n h a n h t h u d u a c t r o n g thi n g h i e m
; ke't q u a b a n g 3 c h o tha'y t y Ie t n i n g t y Ie p h d i t h o a t m a n g / s o t n i n g n u d i k h d n g cd t h u c , s o t n i n g n u d i s a u cay n h a n ciia s u k h a c b i e t (75,8 v d i 78,6%; 11,3 v d i 11,7%
Til 1
t h a n h t h u c , s d t n i n g n u d i s a u cay i
h a i n h d m la k h d n g cd s u k h a c biet. V e t y le v a 4,1 v d i 3,9%). T y Ie p h d i p h a n chia va t y le p h o i p h a n chia, ty le p h d i n a n g / s d t n i n g n u d i , h i n h t h a n h p h d i n a n g t r o n g n g h i e n ciru ciia
84 }CHK^^'"- — " • • - " '^^^ - ' ^ -
CHAN NUOI DONG VAT VA CAC VAN DE KHAC
chving tdi cao hon so vdi nghien ciiu ciia Liu va ctv (2014) (70,3 va 7,9%.). Tuy nhien, sd te bao/phdi nang ciia nhdm six dung ddng te'bao Ipn due tha'p hon so vdi nhdm sir dung ddng te bao Ion cai (45,1 va 37,5 te bao) (P<0,05).
Nhu vay, kha nang phat trien thanh phdi nang giiia 2 nhdm sir dung te' bao due va cai lam te'bao cho la khdng cd su khac biet tuy nhien eha't Iugng phdi d nhdm sir dung ddng te'bao cai la cao hon so voi nhdm sii dung ddng te' bao due.
Yoo va ctv (2017) tie'n hanh theo doi anh hudng cua gidi tinh ddng te' bao den su phat trien aia phdi in vitro va in vivo cho tha'y ty Ie phdi nang trong tao phdi nhan ban vd tinh in vitro (d te'bao Ion cai la 11,9% va lgn due la 11,3%), cao hon so vdi in vivo (d te'bao Ign cai la 7,2% va lgn due la 10,6%). Nhu vay, ty 1| hinh thanh phdi nang sir dung te' bao Ign d^c va te'bao Ign cai trong nghien cuu ciia Yoo va ctv (2017) tuong duong vdi ket qua trong nghien ciiu cua chiing tdi.
Nghien cuu trudc day cho bie't ty le chet bat thudng ciia Ipn con sau sinh dupc tao ra tir ddng te bao sinh dudng Ipn due cao hon so vdi te' bao sinh dudng lgn cai (Yoo va ctv, 2017). Trong so cac Ign con dupc nhan ban da chet trong vong hai tuan sau khi sinh, ty le chet d nhdm sir dung ddng te' bao vd tinh due eao hon so vdi ddng vd tinh cai (41,2 so vdi 33,3%). Cac dii lieu thu dupc tir cac nghien aiu tnrdc do thi kha nang tao phdi nhan ban tir te' bao sinh duong cd the do sir khac biet cua eac ddng te'bao eho. Cu the^ su ba't thudng ve kieu hinh cua Ign nhan ban cd the do tac dpng ciia qua trinh nudi cay in vitro cua cac te'bao cho. Tuy nhien, ddng te'bao due cd gay chet sdm cho lgn con hay khdng thi can phai nghien cuu them. Cho deh nay, chua cd ldi giai thich nao cho su khac biet ve nguyen nhan gay che't cua Ipn con dugc nhan ban tir hai ddng te' bao due va eai, do do eac nghien ciiu tie'p theo la can thie't de lam rd van de ciing nhu dua ra bien phap ky thuat Iam tang ty le sd'ng sdt ciia lpn con nhan ban vd tinh.
4. KET LUAN
Ty Ie tao phoi nhan ban dupc tao ra tir te' bao lgn due va cai la khdng cd su khac biet, nhung tir cac chi tieu theo ddi cho thay cha't luong phdi cua ddng te'bao lgn cai td't hon so vdi dong te' bao I^n due. Nhu vay, gidi tinh ddng te'bao cd anh hudng nhat dinh de'n kha nang phat trien ciia phdi lgn Ban nhan ban vd tinh. De nang cao ke't qua nhan ban vd tinh, cd the sir dung ddng te'bao cua con cai lam te'bao cho. Ket qua eiia nghien ciiu gdp phan cho viec sii' dung ddng te'bao cua nhiing gid'ng lgn ban dia Viet Nam nham muc dich bao ton da dang sinh hgc.
LOII CAM UN
Cdc tdc gid xin chdn thanh cam an TS. Bui Xudn Nguyen, TS. Nguyen Thi Hdc vd cdc ddng nghiep tai phong Cong nghe phoi Vien Cong nghe sinh hgc dd dong gdp y kieh vd/hodc giup dd thuc hien nghien cdu nay. Nghien cdu duac tdi tra tif di'tdi NCKH cap Vien Cong nghe sinh hgc CS21- 19 "Anh huong cua gidi tinh dong te'bao ca'y len su phdt trim cua phoi lgn Bdn nhdn bdn vo tinh ".
TAI UEU THAM KHAO
1 Boquest A.C, Gnipen C.G., Hamson S.J., Mcllfatrick S.M., Ashman R.J., d'Apice A.E and NotHe M.B.
(2002). Production of Cloned Pigs from Cultured Fetal Fibroblast Cells Biology of Rep, 66(5) 1283-87.
2 Bui X.N., Nguyen T.U., Nagai T., Nguyen V.L., Quan H.X., Nguyen V.H., Nguyen T.T., Nguyen H.D. and Bui L.C (2006) Pruduction of Ban mmipig embryo by somatic cell nuclear transfer, J, Rep Dcv„ 18(1) 147.
3 Dang-Nguyen T.Q., Tich N K., Nguyen B.X., Ozawa M., Kikuchi K., Mannabe N., Ratky J., Kanai Y. and Nagai T. (2010) Introduction ofvanous Vietnamese indigenous pig breeds and their conservation by using assisted reproductive techmques. J Rep. Dev., 56 31-35 4 Kurome M., Ishikawa T. and Tomii R, (2008).
Production of transgenic and non-transgenic clones in miniature pigs by somatic cell nuclear transfer J Rep Dev, 54'156-63
5 Liu H.Lv.?., Zhu X., Wang X., Yang X., Zuo E., Lu Y., Lu S. and Lu K. (2014), in vitro development of porcine transgenic nuclear-transferred embryos derived from newborn Guangxi Bama mini-pig kidney fibroblasts In Vitro Cell Dev Biol Amm, 50(9)- 811-21 6 Nguyin Viet Linh, Tamas Somfai, Nguyen Thi
Nhung, Nguyen Thi Hong va Kazuhiro Kikuchi (2018) Nghien ciVu nang cao kha nang thu tinh ong nghiem sir dung tinh triing lgn Ban dong lanh. HN HKCN sinh hoc toan quoc, trang 1771-75, 7 Polejaeva LA., Chen S.H., Vaught T.D., Page R.L. and
Mullins J. (2000) Cloned pigs produced by nuclear transferfrom adult somatic cells Nature, 407' 86-90
KHKT Chdn nuoi so 256 - thdng 4 ndm 2020
Prather R.S., Sims M.M. and First N.L. (1989) 840^48.
Nuclear transplantation in early pig embryos. Biol „ Y„„ j Q Kim B W Park M R., Kwon D.N., Choi y - " t " ' " V.J., Shin T.S, Cho B.W., Seo J., Kim J.H. and Cho
H T ^ Q";„ H T U ™ v^. p"-"s.uSm »•« <»'^ -^-^ °' -™'«^™' "»r: ::"
L.D., Nguyen T.K.C. and Kikuchi K. (2019). ">«> ""> '" " " '•"i"y d e - t o P ™ " ' ' " " " " " f Cryopreservation o( immahire oocytes of the somatic cell nuclear transfer in pig. Asian-Australas J.
indigeneous Vietnamese Ban pig Amm. Sei J., 90(7)' Amm. Sci, 30(4) 585-92
ANH Hl/ONG CUA FHl/ONG THLfC CHAN NUOI DEN SINH TRUdNG VA HIEU QUA KINH TE TRONG CHAN NUOI DE
TAX THAI NGUYEN
Tif Trung Kien'*, Trdn Thi Hoan\ Bui Nggc Son', Nguyen Thi Minh Thuan', Cu Thi Thuy Nga^- Ho Thi Bich Nggc'vd Trdn Van Htong^
N g a y n h a n bai b a o : 31/12/2019 - N g a y n h a n bai p h a n bien: 05/01/2020 N g a y bai b a o d u o c chap n h a n d a n g : 08/01/2020
T O M TAT
Nghien cmi nay n h a m xac dinh anh hu'dng cua phucmg thiic chan nuoi den sinh t r u o n g va hieu qua kinh te'trong chan nuoi de tai Mo hinh Chan nuoi de ciia khoa Chan nuoi Thu y - Truong Dai hoc N o n g Lam Thai Nguyen n h a m m u c tieu chimg minh hieu qua kinh te'cho nguoi chan nuoi.
Thi nghiem tren 18 de d u e 6 thang tuoi, co khoi luong luc bat d a u thi nghiem la 12,50; 12,58 va 12,48 kg/con (P>0,05), t u o n g iing voi 16 1,16 2 va 16 3. L6 1: de d u a c chan tha t v do, khong bo sung t h u c an tmh; 16 2 d e d u o c chan tha t u do, bo sung 300g ngo nghien/con/ngay; 16 3 d e d u o c nuoi nh6't, cho an tu d o t h u c an xanh, bo sung 300g thii'c an hon hop/con/ngay. Ke't qua nghien cuu cho thay khoi l u g n g ciia de liic ke't thuc thi nghiem 616 1 la 16,78kg, 16 2 la 17,63kg; 16 3 la 20,08kg. Sinh t m o n g tuyet doi lan lugt la 47,59; 56,30 va 84,44 g/con/ngay; sinh truong t u o n g doi la 29,34; 33,77 va 46,88%. Hieu qua kinh te'cua cac 16 tii' cao xuong tha'p lan luot la 16 3 lai 3.603.483VND, sau d o la 16 1 lai 1.168.533VND va thap nhat la 16 2 iai 746.133VND, Ket qua so sanh thong ke da p h a n cac chi tieu o i a 16 3 deu sai khac co y nghia thong ke voi 16 1 va 16 2 {P<0,05), n h u n g giiia 16 1 va 16 2 kh6ng sai khac nhau co y nghia thong ke (P>0,05).
K e y w o r d s : Chdn nuoi, de lai, hieu qud kinh te', phu-ong thdc ciian nuoi, sinh trudng.
ABSTRACT
T h e study of the effect of raising methods on the growth performance and growth profit margin of goat production at Thainguyen
The study of the effect of raising methods on the growth performance and growth profit margin of goat production was conducted at the Goat Breeding Model of the Faculty of Animal Science and Veterinary Medicine - Thai Nguyen University of Agriculture a n d Forestry in order to prove the its effechveness to economic results for farmers. A total of 18 male goats at 6 m o n t h s of age h a d beginnmg of the experiment of Lot 1,2 and 3 were 12,50,12.58 and 12 48kg (P>0,05) divided into 3 lots' Lot 1 was free-grazed, without supplemented concentrate; Lot 2 was grazed freely, supplemented with 300g of crushed com per head when returning to the stable. Lot 3 was kept in
' TiTtoiig Dai hoc Nong Lam Thai Nguyen - Chi cue QLCL Nong lam san va thiiy san Tuyen Quang
* Tac gu-i lien he PG5 TS. Tu Trung Kien, Pho tajong khoa Chan nuoi Thu y-Tru6ng Dai hgc Nong Lam Thai Nguye 0902 119828, Email; tutrungkieniS'tuaf edu.vn
