HOl NGHI KHOA HOC CONG NGHE SINH HOC TOAN QU(5C 2013
VI N H A N G I O N G LOAI THU'C VAT THLIY SINH LUAN T H A O Bd [ROTALA WALUCHII HOOK. F.)
Hong Vu Thuy Uyen , BiJi Van Le
Tneimg Dai hoc Khoa hgc Tir nhien, Dai hoc Quoc gia Tp. Hd Chi f/linh
TOM TAT
Luan thao do (Rotala wallichi) ia mot loai thyc vat thuy sinh dang duoc ua chupng tren Ihi tnrcmg cay thuy sinh trang tri trong cac hi ca nhan tao Viec nhan gi6ng cay phuc vu nhu chi chira duac nghien cmi nhiSu, chii y6u diroc khai thac chu ylu trong cac ngu6n tv nhien gay ra nhiing nguy co Iam ton hai din he sinh thai vung, Trong nghien cCru vi nhan gifing loai Uiirc vat thuy sinh nay_, chimg toi sii dung cac chfii Rolala wallichi tren mat nuoc lam vat lieu khm diu cho quy trinh vi nhaii gifing bang choi dinh. Mau cay dugc dat tren mfii truong MS bfi sung sucrose 30 g/l, dilu kien chiSu sang !6 giuAigiy, hai ySu to dugc khao sat la chat dieu hoa sinh tnr&ng thuc vat (BA, NAA, TDZ, KIN, GA,) va cac loai gia fhi (klifing gia thi, gia the gon, gia the agar). Sau 6 tuin nufii c4y, BA u nfing do 1 mg/l cho thdy hieu qua tfii iru cho viec nhan chfii. Choi tang lnroTi| tfit va hinh thanh rS bit djnh tren mfii tmong khong bfi sung chit dieu hoa smh trudng thuc v^t sau 4 tnln. Ket qua cho thay gia the co anh hudng daog ke den sir tiiay dfii hinh dang ben ngoai ciia chfii Roiala wallichii nuoi c3y trong di6u kien in vitro. Cay con hoan chinh co ti le sfing 100% trong difiu kien be nuoc nhan tao
Tir khda chit diSu hoa sinh trudng thuc vat, gia the, Luan thao do, Lytliraceae, Rotala wallichii, vi nhan gifing M6E)AU
Trong nhO'ng nam gan day, cac loai thirc vat thuy smh dang du'gc tap trung khai thac SLF dung trong viec trang tri cac be ca tao nen mot nganh cong nghiep mang lai gia tri kinh ti cao. Nhu- 6 chau Au, nhu cau si> dung cay thOy sinh dung de trang tri lien tyc tang gap doi moi nam ke tu" nam 2000 (Winkenmann et al., 2006), d" Snianka, no'i san xuat thyc vat thuy sinh trang tri Id'n nhat thi giai tif nam 1997 ddn nam 1998 da tang gSn gSp doi lu-gng cay xuSt khau tCr 4,2 trieu cay (1997) den 8 tri^u cay (1998) (Yapabandara etal., 2006).
Luan thSo da Ro(a/a wallichii Hook, F., mot loai thuy sinh cd nguon gdc ty nhien tai Viet Nam, dang Quae khai_ tiiac nhu"
1 loSi thuy sinh trang tri dugc thi tnro'ng ua chugng, mang lai gia tri kinh ti cao Tuy nhien, cung nhU' mot s6 loai thuy sinh khae, chua cd cfing trinh nghien ciru nao v l ap dung ky thuat vl nhan gilng trong viec san xuat thu'O'ng mai doi luvyng nay, nguln cung cap tren th] tru^ang deu dugc khai thac ty nhien va nhan gilng truyln thing cd he s6 nhan thip va gay ra nhu-ng nguy ca v l mat can bang he smh thai. Ben canh do, da cb mot nghien ciru v l m6t s6 hgp chat thu" d p tren loai thyc vat cung chi la Rotala rotundifolia vai tilm nang O'ng dung trong y hgc rlt lan (Tan et al., 2009). Vi vay, chung tQi liln hanh irng dung ky thuat vi nhan giong tren Rotala wallichii nham muc ti§u: bao t i n , san xult cay giong, cSy con phuc vu cho thi trugng tiiuy sinh trang tri, han chl viec tin hat din moi trirgng sinh thai va phyc vu cho cac nghien CLPU hoa hoc, sinh hda tilp theo tren dli tirgng nay.
NGUYEN LIEU VA PHU'ONG PHAP NguySn lieu thirc vat
Nguln mlu ban diu la chli dinh Rotala wallichii duac thu hai tir Vugn Quic Gia L6 Go - Xa Mat.
Dilu kien thi ngtiiem
Nhiet 66 ciia phong nudi cay la 25 ± 2°C, cuang dp chilu sang khoang 950 - 1050 tux, thoi gian chieu sang 16 gig/ngay.
Moi trirangnuoi cly 1^ moi trucrng MS (Murashige va Skoog, 1962) co bo sung 30 gfl succrose, pH 6,3 ± 0,1.
Phu'O'ng phap khi> trung
Phln chli dinh dai khoang 2 - 3 cm (Hinh IA) dugc ri>a sach bang xa phong loang va nu'd'c may. Sau do m l u tilp tyc dugc xu ly trong tu cly vo trung lan lugt bing cln 70° (1 pliiit), dung dich Javel (Super Sodium Hypochioride va Sodium Hydroxide) vai ty le Javel;Nu6'cla 1:3 (15 phiit), sau do rira lai bing nu-oc clt vo tning va Sen hanh nuoi cly.
Mlu sau khi dugc-nj-a-bing nuac clt vo triing du'gc cat fao phln g l c da bj Javel aft mon, con kich thirac 1 , 5 - 2 "cm, dugc difa vao mQi tn/dng MS, bo sung 30 g/l succrose, nudi cly trong vong 4 tuan, de lam vat lieu dau cho tat ca cac thi nghiem trong nghign ciru nay (Hinh IB).
Phu-cng phap thi nghiem
vat lieu choi dong nhlt cd kich thu'd'c 0.5 - 1 cm du-ac nuoi cly tren moi tru-ang MS b l sung chit dilu hoa sinh tru-ang thu'c v4t vgi nong d6 khae nhau tuy ttieo nghigm thu-c. Cac chit dieu hoa sinh trirong thyc vat nhom cytokinin (6- benzyiadenin - BA. Kinetin - KIN, N-phenyl-N'-1,2.3-thidiazol-5-y!urea - TDZ) du-gc si> dung detheo doi sy nhan chli cua mlu cly ban diu. Trong thi nghiem khao sat qua trinh tang tnj-ang chli v^ hinh thanh rg, 2 chat d i l u hoa sinh tru'O'ng duo-c bo sung v^o moi tarcyng tin lu'at la Gibbereliin - GA3 va o-naphthalene acetic aad - NAA. De khao sat sy thay doi hinh thai cua loai thyc vat thuy sinh Rotala wallichii trong dilu kien in vitro, thi nghiem dirge Ihu-c hien bang sy thay dll gia t h i agar bang gdn va khong sir dung gia the.
^ _ _ ^ ^ H O l N G H ! K H O A H O C C O N G N G H E S I N H H O C T O A N . Q U O C 2 0 1 3
Cac cay c o n k h o e m g n h c 6 k i c h t h u o c h u n g binh 3 - 5 cm d u w c n>a sgch dung lam n g u l n vgt lieu t r l n g Irong c a c b e
P h i r o n g p h a p x i r l y s o l i e u
Q P ^ ' h i ^ ' ^ ^ ^'^^^ ' ^ ^ '^' ^ ' ^ " ' ^° ' ' ^ " ^ ^ ^ ^^ "y ^ ^ " 9 P*^^" f " ^ ' " ^ P S S ''^•O- ^^^ ^ " ^ ^^ ^ ' ^ " * * " ' ° ' ' ^ ^ " 9 ^'•""9 ^'"^
± b h , phSn n h o m cac gia In bang p h u g n g phap Duncan'test (Duncan, 1995) v d i P = 0,05.
K £ T Q U A V A T H A O L U A N
K h a o s a t a n h hu-o-ng c u a loai v a n o n g d o c y t o k i n i n ten k h a n a n g h i n h t h a n h c h l i
r ^ " ^thA^"^ ™ ^ ' ^ ^ ^ ' '^^ ^ '^^'^ nghiem thi>c c l s y b l sung cua B A d i u c6 s u cam u-ng tgo c h l i , c h l i phai smh tCr n a c h ia, va khdng co s y tao re. O m6i tru-ang co bo sung TDZ thi s y c a m u n g tgo c h l i dien ra sau h g n 14 ngay va co s y h i n h thanh s ? o a day. D o i vd'i bo sung KIN thi kh6ng co su- cam i>ng tao c h l i , va co s u hinh thanh re sau 2 0 n g i y va n g h i e m t h u c doi chu-ng thi r& bat djnh phat sinh s a u 7 ngay nufli c l y , khong c 6 s y hinh tfiinh c h l i .
Qua do c h o m ^ y . BA c h o k i t qua c a m i r n g phat sinh choi t i t h a n vd'i T D Z va Kinetin a ciing n i n g dO (1 va 2 mg/l). M p t so ngnien cu-u cOng da cho k i t q u i tu-ang t y , nhu- doi vd'i s y nhan choi Cajanus cajan in vitro cho t h i y BA c 6 hi#u q u a nhat, Uep d e n la Kinetin, Zeatin, Adenine, hay trong nudi cay c h l i . m o phan sinh, choi ngpn va c h l i ngu a Manihot esculenta Crantz (Nair e( a/.,1979; Shiva etal.. 1994). Va cung cho t h i y , s y irc c h l ciia BA ien qua trinh tgo r l vd tang t r u a n g c h l i c u a Rotala wallichii in vitro d cac n i n g d p cao (5 va 10 m ^ ) . H i ^ n br<?ng tu-ang t y cung d a d u g c ghi n h a n trSn nhieu doi tu-gng t h y c vgt khae n h u Artemisia vulgaris L. (Sujatha et al., 2007), Fragaria indica A n d r (tndhra et al., 2000) ya Anubias barieri var. nana Engler (Hoang et al., 2009). Tir k i t q u i thi nghigm cung c h i r n g minh l u g n g auxin ngi sinh cua m a u c l y la cao, co kha nang c h o ra r l tren iJeu ki§n moi tm-ang MS khdng co bo sung c h a t daku hoa sinh t r u d n g t h y c v ^ t . Ket lu$n trong thi nghigm khao sat 3 logi cytokinin thi vigc sCi- dyng BA if n i n g dif 1 mg/l cho ket q u i t g o c h l i Rotala wallichii tot nhat, he s l nhan choi tu-ang d l i cao (7,08 ± 0.46) v a tang t r u a n g c h i l u cao t i t n h l t (0.75 ± 0,11 cm).
Dieu nay c h o t h a y n i n g do BA sii- dung c h o q u a trinh nhan c h l i c u a Rotala wallichii thip han han so v a i qua trinh n h a n _Pln6i cua mnt R I ioai t h y c vgt thuy slnh khae t h u i n ig k l i u j i i g 2 0 p M b A da du-gc nghien c u u trgn t h i g i l ' i nhu-
Cryptocoryne wendtH (Kane e( al.. 1999), Cryptocoryne lucens ( K a n e e( al., 1990), Nymphoides indica (Jenks e ( a/..
2000).
Bang 1. Ket qua anh h u ^ n g cixa nong do va loai Cytokinin (mg/t) t6n qua trinh nhan choi ciia Rofa/a wallichii Nfing Sif
0 1 mg/l BA 2 mgfl BA 5 mg/l BA 10 mg/l BA
S6 chli 1,03 ± COS' 7,08 ± 0,46"
7,22 ± 0,78"
6,67 ± 0,81"
11.66±1,13'
C h i l u cao chfii (cm) 2,32 ±0,38"
0,75 ± 0 , 1 1 "
0,54 ± 0,08°
0,36 ± 0.04"
0,23 ± 0,02"
Nfing fl$
0 1 mg/IBA 2 mg/l BA 1 mg/l KIN 2 mg/l KIN 1 mg/l TDZ 2 mg/l TDZ
sfi chfii 1,03 ±0,03°
7,08 ± 0.46' 7,22 ± 0,78' 1.00 ±0,00' 1.00 ±0,00' 4,43 ± 0.57"
4,37 ± 0.63"
Chieu cao chli (cm) 2.32 ± 0.39' 0,75 ±0,12"
0.54 ± 0.08"
2.37 ± 0 , 1 5 ' 2,43 ± 0,19"
0,39 ± 0,02"
0,28 ± 0,01"
Chli thich. Nhu-ng chi> c i i khae nhau (a, b. c ..) du-oc n&u trong cac cgt bieu d i l n s y khic nhau c6 ngtiTa voi a = 0,05 trong Duncan's
K h a o s i t a n h h u - d n g ciJa GA3 v a N A A ten k h a n a n g t a n g tru'O'ng c h o i v a h i n h t h a n h re in vitro
D l i vd'i qua trinh tang t r u a n g c h l i , s a u 4 t u i n nufli cay cho t h i y , mfli tru-ang nufli cay la M S c 6 b l sung 1 , 2 hogc 5 mg/l GA3 cho thay s y tang c h i l u c a o c h l i so v a i vigc khong b l sung GA3, v a tang tru-dng c h i l u cao t i t nhat ir nong do 5 m g / l G A j var 3,91 ± 0,94 c m . Tuy nhien, trong qua trinh t h u c hien t h i nghiem, viec hap khu- trung dong t h a i v a i mfll triFcrng gay nen s y t h i t thoat idn vi khflng the kiem soat, k h i l n eho k i t q u a xu-1;^' t h i n g ke khong cho t h i y s y kh'ac bigt g i i r a vi^c b l sung v a khflng bo sung GA3 v a o moi t n r a n g nufli c l y la khflng dang ke. Tuy n h i l n , ve so sanh cSc gia tri t r u n g binh cho thay, GA3 cd tac dOng l&n su- tang t r u d n g cua c h l i d 2 n i n g do la 2 hay 5 mg/I GA3.
Trong q u a trinh quan sat v a ghi nhSn k i t qua hinh thanh re in vitro, ta nhgn t h i y d l i v 6 i dc m l i t r u i n g cfl bo sung N A A qua trinh nay d i l n ra nhanh h a n so v d i t h d i gian 7 ngay cua m a u k h f l n g b l sung NAA, va cfl s l t u g n g nhieu h a n , T u y nhifin, dc r l nay thu-dng ngan, dj dgng va phinh t o , gan nhu- bj thoi nhung khi d u a ra moi tru-dng ex vitro. Mfli t n j d n g nuoi cay ta M S khong co b l sung NAA thi rnau cay co s y hinh thanh re it, c h i l u dai 2,04 ± 0,03 cm Moi trirdng n u l i d y
•a MS CO b l s u n g 1 ho$c 2 mg/l NAA thi m a u c l y c h o t h i y s y tang s 6 t u ^ n g re so v d i khdng b l sung NAA, so lu-gng r l cao nhat d n i n g dO 2 mg/l NAA 11,32 ± 1,05. Dieu nay cho thay. ham l u g n g auxin ngi sinh d m l u cay Rotala wallichii la cao, lam cho m l u c l y cd the tgo re b i t i^nh trong moi^tardng khflng ed bfl sung NAA, m l u r l cfl hinh dgng binh thu-dng, manh v a khoe. T u y t y t§ auxin/cytokmin > 1 se giiip m a u cay tao re, n h i r n g d day v d i vigc nong d g NAA bo sung k i t h g p v d i t i / g n g I d n auxin nfli sinh d i n d i n vific k i c h thich mgnh su- hinh thanh s g k h d i re, nhu-ng ire d i e vigc keo dai c i i a eac s o k h d i 1^ nay ISm cho cac re d mfli tru-dng c d b l sung NAA cd dang n g i n va phinh to (Vi§t. 2000).
HOI NGH! KHOA HOC C O N G NGH? SINH HOC TOAN Q U 6 C 2013
B i n g 2. A; ket qua anh h i r d n g cua nong do GA] (mg/l) ten gui trinh tang t r i r d n g ; B: ket qua anh h u v n g c (mg/l) hinh thanh re ciia choi Rotala wallichii in vitro
i n 6 n g d o NAA
Chilu cao chfii (cm) s l lu'gng r l Chieu d i i rS (cm)
1 mgfl G A J 2 mg/l G A J 5 mg^ GA3
2.39 ± 0.25*
2.89 ± 0.53' 3.25 ± 0.97"
3,91 ± 0,94'
1 mg/l NAA 2 mg/l NAA
5,94 ± 0 . 0 7 ' 2,04 ± 0 , 0 3 ' 9,03 ± 0 , 7 6 ' 1,01 ±0.05"
11.32 ±1,05* 0 . 6 4 ± 0 , 0 1 '
Cha thich. Nhirng chO c i i khic nhau (a, b, c . ) diroc nSu trong c i c cOt b i l u dien S^F khae nhau cd nghTa vdi o = 0,05 trong Duncan's
Hlnh 1. A: Chdi dinh Rotala wallKhiitikn xi> ly; B: nguon mau Rotala wallichii In vitro sau 6 tuan num cay; C: s i r nhan choi cua Rota/a wallichii t r i n mdl t n r d n g c6 bo sung c i c loai va nong do cytokinin khae nhau.
(BAo 0 (dll chiing); BA,' 1 mg/l BA; BA,: 2 mg/l BA BAs: 5 mg/l BA; BAioi 10 mg/l BA; Ki,' 1 mg/l Kin; Ki,- 2 mg/i Kin; TDZ,: 1 mg/l TDZ, TDZ,: 2 mg/l TDZ)
Hinh 2. A; S y tang tru-d-ng cua choi Rota/a wallichii In vitro tren c i c mdi t n r i r n g c6 bo sung nong do GAj (mg/t) k h i c nhau; B:
S y hinh t h i n h r l cda c h l l Rota/a wallichii in vitro tren c i c mfli tru-dng c 6 bo sung ndng d d NAA (mg/l) k h i c n h a u . (GAo, N/Wo" 0 (ddi chCrng); GA,: 1 mg/l GA,; GA,: 2 mg/i GA,; G/^s: 5 mg/I G/V,; NAA,: 1 mg/l NAA; N/W,: 2 mgli NAA) K h a o s a t a n h h y d n g ciJia dg r a n m o i t r u w i g ten s y t h a y d l i h i n h d g n g c u a c h l i In vitro
ThI nghifim du-gc ghi nhan k i t qua s a u 14 n g i y nufli cay. & m6i I n g nghifim d i u chi>a l y g n g khoSng v a cac c h i t dinh d u ^ n g b a n g n h a u . chf c d gia ttii khae n h a u . K i t qua da cho thay su- t h a y doi hinh thai khSc nhau ciia c h d i Rotala _ _ wallichii in vitm trong s y hinh t h a n h d a n g cay, s y tgo choi, hinh dgng ii, h i n h dgng r l d l i v d i tirng logi gia t h 4 nufli c a y ,, khae nhau. O m d i tru-dng dCing gia t h i ta agar va "agar + HzO v f l triing", v d l ciing gia t h i n l n la agar, c h o t h i y bO r l I , phat t r i l n g i l n g n h a u , v d i dc s g i manh dai. (3 m l i trirdng d i i n g g i a t h i agar khflng b l sung HzO phfa trfin, cSy 06 ta , , , n h d , xanh d a m , t h a n c d n g c a p , v a tang tru-dng c h l i k h l n g n h i l u . CS moi t n / d n g dung gia the agar c6 t i l s u n g H j O , cSy
I c d la dai, x a n h n o n , than m i m h a n va tSng t n r d n g c h l i t i t .
'• I S y khae bifit ro r#t v e hinh d g n g c a y du-gc ghi n h ^ n trgn mfli t r y d n g khdng gia t h i vi c6 gia the la agar. 6" m 6 i tru-dng ' k h d n g gia t h e . h l n h dang thSn la du_di dgng ngap chim cho dgng ta dai, mflng, than m i m , khdng djnh hinh d u - g c h i n h ' ' d a n g , c d s y phat sinh c h l i n h a n h ; r i d i r g e tiep xiic t r y c tiep v d i a n h sang ndn c6 mau xanh d i m , to va ngSn. K h a e s o
• v d i m d i t n r d n g dCing gia t h i la agar v d t dgng la nhd, xanh d a m , t h a n c d n g , h u d n g t h i n g ten trSn, rfi c d m a u t r l n g va x a n h .
HOI NGH! KHOA HOC CONG NGH$ SINH HOC T O A N Q U O C 2013
Ngoai ra. sy khae biet edn du'gc dugc nhan trfin 2 loai moi trudng diing gia the la gdn va khdng gia the. O 2 loai mdi
^arang nay deu la moi truo-ng long, nhung khi dugc bo sung gia the gdn, mlu cly ngoai hip thu dugc dmh dudng dudi
~- '- ' •' • ' - • • iiHii ixji bdng gdn nen Knong phat trien ty do nhu- d mdi inrang khong gia thg. Chinh vi vay, mau mfli tnro'ng diing gia the ta gdn cho hinh dgng than mim, mdng manh, la khdng dipgc mg rgng tOi da, cd sy phan nhanh va re vi phai ten ldi trong cac sai gdn nfin chac khoe, to va co mau xanh (do su' can anh sang cua gdn la khflng nhilu).
Ttr nhung ket qua tren, vdi nhu-ng muc dich nghien edu khae nhau. ta c6 Ihl irng dung cac nguln gla thi khae nhau. Vi h " k I'"°' ^'^^"^ '°."9 ^^ ^^'^^ ^VP cho vific nufli cly ty ddng bing bioreactor, hay Idng lie, long tTnh nham thu nhan nhanh lugng sinh khdi, dng dyng trong vigc thu nhgn hgp chit thd d p tii- loai thiiy sinh nay; mli tnrdng agar ket hgp vgi nude se thich hgp cho viec nhSn giong Ro(a/a wallichii trong cdng nghe cay kieng, vi chiing gid dugc vdc dang.
thang, dgp cua chimg, khi du-a ra mfli tnrdng ngoai; mdi tnrdng rln khdng nude se duac diing trong vific giu- giflng va cac nghiSn ciru v l sinh ly sinh hfla sau nay.
Bang 3. Ket qua i n h hu-o-ng dd r l n IT li tru-dng len s y thay doi hinh d^ng cita choi Rotala wallichii In vitro.
G i i t h ^ Agar 7,S gli Agar 7.6 g/l + H , 0 vd triing Gon
Khong g i i t h i
Chieu cao ch&i (cm) Chieu dai r l (cm)
3,08 ± 0,06' 2,56 ± 0,04"
2,95 ± 0,09'
3,17 ± 0,03' 3,25 + 0,10' 2,73 ± O.OS"
3,07 ± 0,07*
ChO thich Nhu-ng chu- cai khae nhau (a, b, c ) duij-c neu trong cac cdt bi4u d i i n sir khic nhau c6 nghTa voi o = 0.05 trong Duncan's
Hinh 3. A: Choi Ro(a/a wa/Z/cfi/i/n vitro cd klch t h i r d c l - 1,5 cm trong c i c gia IhS khae nhau (ngay t h i r 0); B : S y thay doi hinh thai Rota/a wallichii In vitro trong c i c g l i the k h i c nhau ( n g i y thir 14).
( 1 : Agar 7,6 g/l; 2. Agar 7.6 g/l + H,0 vd trOng, 3: Gdn; 4' Khdng g i i the) Kha nang song sot ciia ciy con In vitro
Cac cSy con khde mgnh cd kich thudc trung blnh 3 - 5 cm dirge n>a sgch dimg tam nguln vii lifiu trflng trong cac be thiiy smh trfin gia thi la eat sgch vd sfli cho kit qua sdng sdt dat 100%.
K E T L U ^ N
Trong nghien cdu nay, chOng tfli da xfiy dyng duac quy trinh vi nhan gilng loai Luan theio dfl Rotala wallichii vi co dugc mgt s6 so sanh budc diu ve sy thay dli hinh dgng ben ngoai cua loai trong difiu kign in vitro. Trong giai dogn tgo chli cua quy trinh vi nhSn gilng, hifiu qua nhan chli Rotala wallichii dgt tit nhlt trgn mli trudng MS b l sung 1 mg/l BA vdi hfi s l nhSn chli cao (7.08 ± 0,46) va chilu eao chli dat 0,75 ± 0,11 cm. Mdi trudng MS khdng dugc bp sung NAA la moi tnrdng sir dung hifiu qua cho viec tgo re vdi hfi sd tgo rfi (5,94 ± 0,07) va diieu dai re 2,04 ± 0.03 cm. Cac logi gia the cd anh hudng dang ke din sy thay dli hinh dgng bfin ngoai cua Rotala wallichii trong dieu kien nudi cay in vitro. Tir nhdng kit qua cfl dugc, chiing tdi ed the tilp tyc hoSn thidn quy trinh vi nhSn gilng cung nhu cd nhirng nghidn cdu sau han tren loai thyc vlt thuy sinh Rotala wallichii vdi ede gia tri ve san xuat cay thiiy sinh va cac gia tn do cac hgp chit thd cap ma loai nay mang tgi (Tan e( aL, 2009).
T A I L I E U THAIVl K K A O
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MICROPROPAGATION OF AQUARIUM PLANT ROTALA WALLICHII HOOK. F.
Hong V u Thuy Uyen', Bui V a n L e
University of Science, Vietnam National University in Ho Chl Minh City
SUMMARY
Shoot lips on ihe water are used as the first materia for micropropagation protocol of Rolala wallichii. This explants are cultured on MS medium plus 30 g/l sucrose; under 16h photoperiod, two factors focused in this research were plant growth regulators (BA, NAA, TDZ, KIN, GAj) and difiercnt substances (liquid without substance, agar, absoiiKnt cotton wool). After 6 weeks, 1.0 mg/l BA - supplemented MS medium is optimal medium for shoot proliferation The shoots grow and root on MS medium without plant growth regulator after 4 weeks culture. The different substance significandy effected on Rotala wallichii shoot forni. Tlie survival rate of plantlets was 100% in ex vitro environment.
Keywords- Lythraceae, micropropagation, plant growth regulators, Rotala wallichii, substances
•Author for conrespondence: [email protected]
