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V A Streptococcus s p p CIJA DICH CHIET M d T SO LOAI T H A O DUOC
Trin Nggc Hiing', Tbiong Thi Thinh Vmh^, T i i n Thi Lam Giang^
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K^t qua nghidn cihi cho thiy cac Ioai dung mdi anh huong d ^ boat tinh khang khuan ciia thao dugc. Khi su dirng ethanol vA metanola d^ chiet xuat dich chiet se cd kha nSng khang vi khuan V. vulaidcus va Streptococcus spp cao hon so vdi dung m6i nudc va clorofom. Vdi dung mdi ethanol vi methanola kha nang khang khuin cua dich chi^t cu t6i d^t tdt dudng kinh vong khang khuin 20,12 mm - 21,71 mm, dich chiet la di$p h^ chau dat tnmg binh, dudng kinh v6ng khang khuin 14,61 mm -16,23 mm. Ri6ng ddi vdi dich chiet la hoan nggc trong tit ca cac lo^ dung mSi d^u cd muc khang khuan k6m, dudng kinh vdng khang khuan 4,75 - 9,09 mm. Ndng d6 uc ch^ ^ i liii^ ddi voi V. vuhiScusva Streptococcussp^ cua dich chi^t cu toi khi sii dyng dung mdi ethanol, metanola vdi MIC = 64 jig/ml, nudc vdi MIC = 128 pg/ml, clorofom vdi MIC lin lugt la 256 pg/ml va 512 pg/ml. Dich chi^t la di6p ha chau vdi dung mdi metanola c6 ho?it tinh khang k h u ^ trung binh vdi MIC la 1024 pg/ml, cac dung mdi c6n lai co miic khang khuan y^u vdi MIC dao dgng tu 2048 pg/ml d^n 4096 pg/ml
Tii khda: Toi, li di$p ha chau, li hoin ngpc, dudng kinh vdng khang khuan, dich chidt, MIC.
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Hidn nay, vide sii dung thio dugc d^ thay tiid thudc khang smh frong phdng va trj bdnh frdn ddng vat thiiy sin dang duge nghidn eiiu, iing dvmg nhidu frong thi;e ti^n sin xuit. Da cd nhidu kdt qui chiing minh ring khang sinh cd ngudn gdc thio dugc ed tae dimg tri b^nh khdng tiiua kdm cic loai khang smh t<Sng hgp, dac bidt khdng gay hidn tugng khang tiiudc va than thidn vdi mdi hirdng [4], [6].
Cii tdi {Alh'um sativum L), cay didp ha chau {Phyllanthus amarus Sebum va Thorm) va eay hoan nggc {I^euderanthemum bracteatmii) \a nhiing Ioai thio dugc da dugc sii dung nhi^u trong phdng va chaa trj b§nh cho ngudi va vat nudi [4], [7]. Tdi dugc bi^t d^n vdi cdng d^mg nhu chdng cic bgnh do vi khuin, nim, virut, phdng tri cac bdnh ciim, dirdng mdt, di^u bi vdt tiiuong,... [4], [11]. Cay didp ha chau dugc dan gian sii dimg rit nhidu frong ehua tri cic b$nh vd gan nhu suy gan, xa gan c6 trudng [9], C^n cay hoin nggc hay cdn ggi la cay eon khi dugc biA d^n nhu mOt lo?i thuoc "k^ didu"ehira cac bdnh
' Tnrdng Cin bd QuAn 1^ Nong nghidp \k Phat trien Ndng thdn I, BO Nong Nghi^ va Phat Uien Ndng Uidn
* Khoa Ndng LSm Ngu - Tmdng D^i bgc Vinh ' Ldp K 20 Sinh hge thvc nghi?m - Trudng Dpi hge Vinh
NdNG NGHIEP VA PHAT TRIIN NdNG THdN - KY 1 • THANG 9/2014
v^ dudng tidu hda, dau da day [4]. Chinh vi th^, frong nudi trdng thuy sin ciing da cd mgt sd nghidn Cliu thu nghidm vd kha nang khing khuin cua cd tdi, Ii didp ha chau tuy nhidn mdi chi dung dang dich dp nguydn chat Mdt thuc td cho thiy, hoat tmh cua thao dugc khi sii dung ehiu inh hudng cua rit nhidu ydu t^ khac nhau nhu nhi§t dd, cac dung mdi hda tan, phuong phap bio quin,...Do dd, muc dich nghidn ciiu chinh cua chung tdi la xic dinh inh hudng cua ckc Ioai dung mdi khac nhau khi chi^t xuat thio dugc gdm eu tdi, la didp ha chau va la cay hoan ngpc d^n kha nang khang vi khuin Vibrio vuIniScusvi. Streptococcus spp gay bdnh Id lodt frdn ca bdng bdp va ca frd lai.
2. VAT U£U, NQI DUNG VA PHUONG PHJIP NGHiN CtiU 2.1. D|a d i ^ thdi gian v i vat Udu nghidn cihi - Dia dilm nghidn cuu; Phdng Vi sinh Bdnh hge thuy sin, Khoa Ndng l i m Ngu, Trudng Dgi hge Vinh.
- Thdi gian tridn khai nghidn eiiu: Tir 1/3/2013 den 10/5/2013.
- Vat Udu nghidn eiiu:
+ Cli tdi {Allium sativum L).
+ cay didp ha chau {Phyllanthus amarus Schum va Thorm.).
Sl
KHOA HOC CONG NGHfi
+ cay hoin nggc {Pseuderanthemam bracteatum).
+ Cac Ioai dung mdi gdm: Nuoc, ethanol, metanola, clorofom.
+ Vi khuin Streptococcus spp dugc phan ]ap fren ca rd phi {O. niloticus) va Vibrio vuIniGcus dugc phan lap frdn ca bong bdp giai doan thuong phim hi bdnh Id loet (Midi Iugng ca 80 - 1 0 0 g/eon).
Phuong phap phan lap va dinh danh vi khuan tiieo Millar va Frerichs (1993), Buller (2004). Chung vi khuin sau khi da phan lap dugc ciy gift d 4''C trdn mdi trudng dinh duong tiiach nudc xuyt (Nutrient broth agar). Trudc khi su dung ki&n tra tinh thuin thdng qua c i c dac diem hinh thai Idiuan lac, hinh dang kich thudc vi khuin, nhudm gram, cac chi tidu sinh hda ca ban (oxidaza, eatalaza, tinh di ddng, phan ling decacboxylaza, kha nang len men va oxi hda dudng glucoza).
2.2. Ndi dung nghidn cthi
- Nghidn ciiu anh hudng cua cac Ioai dung mdi khac nhau den kha nang khing khuan cua dich chiet cu tdi, l i cay didp ha chau, la cay hoan nggc vdi vi khuin Streptococcus spp v i Vibrio vulnificus.
- Xic dinh nong dd lic che toi thieu (MIC) cua dich chilt cac Ioai thao dugc ddn vi khuin Streptococcus spp vk Vibrio vulmScus.
2.3. Phuong phip nghidn cihi - Phuong phap thu dich chidt thao dupc:
+ Ddi vdi Cli tdi: Bdc vd lua, lay cic i n h tdi va nghidn frong eae dung mdi hiiu ca theo ty lg 1:1 (1 g tdi: 1 ml dung mdi), dich niy dugc bao quan 2 gid b nhidt do phdng, lgc qua g ^ . Dung dich chiet dugc sidu am frong 30 phiit d didu kidn lanh. Tidp tuc U tam vdi tdc dd 3500 vpng/phiit frong 20 phiit Hiit Iiy dich frong, mang di cd quay hiit chan khdng 6.6 loai bd hoan toan dung mdi. Dich chief da dugc loai bd dung mdi dugc bao quan frong tu mat 4"C.
+ Doi vdi Ii cay hoan nggc va la cay diep ha diau cin rtra sach, d l khd tu nhidn sau dd ciing nghien nat ridng eie Ioai la nay trong eae dung mdi theo ^ Id 1:1 sau dd lam tirong tu nhu ddi vdi thu dich chidt cii tdi.
- Thlnghi$m 1: Nghidn ciiu anh hudng ciia cac Ioai dung mdi gdm nudc, ethanol, metanola, clorofom den kha nang khang khuan ciia dich chiet cii tdi, la
cay hoan nggc, la cay didp ha chau voi vi khuin Slfreptocwcrassppva Vibrio vulnificus.
Kha nang k h i n g vi khuin Streptococcus spp vi Vibrio vubuBcus dugc ghi nhan hing dirdng kinh vdng vd khuan cua cac dia giiy tim dich dp bing phuong phip thii khang smh dd ciia Bauer - ICfrby, 1997.
Cac thao t i e dugc thuc hidn frong tii cay vd, trtmg. Khi mat dd vi khuan dgt 10^ t l bao/ml, lie diu binh chiia vi khuan, dung pipet man hut 0,1 ml canh khuin nhd vao giua dia thgch, diing que thiiy tinh i fring ddu cho ddn khi mat thach khd. Sau il5 phiit dat cac khoanh giay vdi dirdng kinh 6 mm cd tim den no dich chiet cua cac logi thao dugc, cac khoanh giay dat each nhau khoang 20 mm. Sau do de vao tii i m d 37''C/24 h dgc k i t qua bang each do dudng kinh vdng vd khuan, rdi tinh s6 hinh quSn.
- Thi nghiem 2: Xac djnh ndng dd lic c h i tdi thilu (MIC) eua dieh chilt cic loai thao dugc din vi khuan Streptococcus spp va Vibrio vuIniScus. (Theo phuang phip cua Truong Cdng Quydn va cdng s(fc, 1986; Tfr Mmh Koongva cgng su, 2001) [3], [5].
2.4. Phuang phap xu ^ s£f Udu
Cac thi nghiem dugc bd tri ngau nhidn hoin toin vdi 3 lan lap. So Ueu dugc xti U thong kd tidn phin mdm Excel. Su dtmg phan tich phuong sai 1 nhan td (ANOV/^ va tidu chuin LSD (Least Significant Difference) de x i c dinh su sai khac cd ^ nghia.
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3.1. K i t q u i nghidn cihi i n h hudng d i a cic logi dung mdi d i n kha nSng k h i n g khuin cua djch chilt cu tdi, l i cSy hoin nggc, Ii cdy didp ha chau
Kit qua d bang 1 cho tiiiy dich chilt ciia 3 logi thao dugc l i cu tdi, la cay hoan nggc va la cay didp h?
chau ddu cd k h i nang khang khuin ddi vdi vi khuin V. vuIniScus vk Streptococcus spp khi chilt xuit bdi cac loai dung mdi nudc, etiianol, metanola vi clorofom.
Qua bang 1 ra do tiii 1 ta thiy dich chilt cii tdi, su dung dung mdi ethanol v i metanola cho dudng kinh vdng khing vi khuin V. vulni£cus Idn nhit, lin lugt la 21,71 mm va 20,79 mm. Str dung dung mdi nudc va clorofom dtrdng kinh vdng khang khuan dd dgt trung bmh, lin lugt l i 18,12 mm, 18,21 mm. Phin tich ANOVA va kilm dinh LSD cho thay cd su sai
82 NdNG NGHIEP VA PHAT TRIEN NONG THON - KY 1 - THANG 9/2014
KHOA HOC C 6 N G NGHE
khac cd y nghia thdng kd giiia dung mdi ethanol, metanola vdi dung mdi nudc, clorofom vdi P<0,05, sai khac khdng cd y nghia thong ke giiia ethanol va metanola, nude va clorofom, vdi P>0,05. Quy luat nay ciing hoan toan tuang tu ddi vdi kha nang khang vi khuin Streptococcus spp ciia dich chief eii tdi. Ddi vdi dung mdi ethanol va metanola dudng kinh vdng khang khuin tot lin lugt la 21,15 mm va 20,12 mm.
Dung mdi nudc, clorofom dudng kinh vdng khang Bing 1. Anh hirdng ciia cac logi dung mdi din
khuin tnmg bmh lin lugt la 17,75 mm, 18,78 mm.
Nhu vay ethanol va metanola da ed kha nang kich hogt dugc hoat Iuc cua cii tdi iam tang k h i nang khang khuan eiia nd ddi vdi vi khuan V. vuIniScus thudc nhdm Gram am va vi khuin Streptococcus spp thudc nhdm gram duong. Kit qua nay la ca sd d l khing dinh dich chief tdi bang ethanol va metanola cd kha nang diet khuan d pho rdng.
kha nang khang khuin ciia dich chilt thao dugc
Thio dugc Dung mdi Dudng kinh vdng khang khuan (mm)
V. vuIniScus Stieptococcus spp Cii tdi
{Allium sativum)
Nudc Ethanol Metanola Clorofom
18,12±0,29^
21,71±0,26'' 20,79±1,29'' 18,21±0,27^
17,75 ±0,74^
21.15 + 0,15"
20.12 + 0,31"
18,78 ±0,25^
La cdy didp hg chau {Phyllanthus amarus)
Nudc Ethanol Metanola Clorofom
10,05i0,09^
14,61iO,62'' 15,10±0,68'' 9,27±0,3y
10,23 ±0,39^
15,55 ±0,21"
16.23 + 0,3"
9,23 ± 0,33^
La eay hoan nggc {Pseuderanthemum bracteatum)
Nude Ethanol Metanola Clorofom
7.92±0.43'' 8,17±0,53"
9,09±0,17=
6,92±0,62'
5.44 ±0.38"
4,75 ±0,46^
4,94 ±0,55^
6,94 ± 0,50^
(Cic chdmO a, b, c trong cimg mdt cot bidu thi sir sai khic cd y nghia thd'ng kd (P<0,05) e i till inh ha«iit<0* otc lofl duntmol dfli khi nMif khiinf khiitr
il 1 1
[8]. Sd dl ed sir khac nhau nay la vi frong tdi, thiosunfat va lim huynh tong sd la chi tieu danh gia chat Iugng khang khuan (Yukihiro Kodera va cpng su, 1995, trich tir [11]). Cac thiosunfat hda tan manh frong cac dung mdi ethanol, methanola cho ndn khi sii dung hai loai dung mdi nay dich chiet tdi se cd dugc hoat tinh khang sinh manh hon so vdi dung mdi nuac va clorofom 111]. Dieu nay cung Iy giai tai sao dan gian hay su dung tdi ngam rugu d l tao thanh loai thuoc udng tang siic d^ khang, chdng eac bdnh Dd thj 1. Anh hirdng cua cdc logi dung mdi
din k h i nang khing khuin cua djch chilt cii tei Nam 2013, nhdm tae gia tiirdng Dai hoc Ndng nghidp Ha Ngi da thu nghiem dung mdi ethanol, methanola chidt xuat djch tdi va xac djnh kha nang khang khuan vdi E.coli O41, kit qua cho thiy dudng kinh vdng vd khuan dat lan lugt 19,1 mm va 19,7 mm, sai khac khdng ed y nghia thdng kd vdi P>0,05 [2]. Thii nghidm djch chilt cae gidng tdi tia, tdi gi6, tdi tring Trung Qudc bang dung mdi nudc cho thiy kha nang khing EcoliYkm, vdi dudng kinh vdng khang khuin chi dgt hi 9,2 mm din 11,3 mm
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Dd thj 2. Anh hudng ciia cac logi dung mdi din k h i nang khang khuin ciia djch chilt la didp hg chau
NdNG NGHllP VA PHAT TRIIN NdNG THON - KY 1 - THANG 9/2014 83
KHOA HOC CdNG NGH|
Ddi vdi djch chilt la didp ha chau, khi su dung ca 4 logi dung mdi tach chief thi deu cd kha nang khang khuan vdi vi khuan V. vuIniScus va Streptococcus spp. Khi dung dung mdi etiianol va metanola, dudng kinh vdng khang vi khuan V.
vuIniScus lin Itrat la 14,61 mm va 15,10 mm. Dudng kinh vdng khang vi khuan Streptococcus spp lan lugt la 15,55 mm, 16,23 mm. Con vdi eac dung mdi nudc va clorofom kha nang khang khuan kem vdi dudng kinh vdng khang khuan dao ddng tu 9,23 mm din 10,05 mm. Phan tich ANOVA va kiem dinh LSD cho thay cd su sai khai cd y nghia thdng kd P <0,05 giua djch chilt la didp ha chau bang dung mdi ethanol, metanola vdi cae dung mdi cdn Iai.
Ciing da cd mgt sd nghien ciiu ve tinh khang khuan cua cay Diep ha chau khi su dung dudi dang djch ep nguydn chat Nghien ciiu cua Huynh Kim Dieu va Ly Thi Loan Em danh gia dac tinh thuin chiing va hogt tinh khang khuan ciia djch dp eay Diep ha chau thu ttr vimg dong bing sdng Cuu Long, k i t qua cho thay loai thao dupc nay chi khang khuan kem doi vdi Vibrio spp [1]. Nhu vay, frong nghien cuu cua chiing tdi khi sir dimg dang djch chief la diep ha chau bang ethanol va metanola thi hidu qua khang khuan tdt ban so vdi dang dich dp va dang b d t Theo nghien ciiu eiia Biller, 1994 diing dung moi ethanol va metanola chiet xuat dich chief la cay cd Lao {Chromolaena odoratd) thi hidu qua khang khuan dat rat tdt. Nguyen nhan la do chung da tac ddng den thanh phin ancaloit cua la cd Lao lam tang boat tinh khang khuan [10]. Nhu vay, rd rang ethanol va metanola ciing da kieh hoat dugc thanh phan ancaloit eo frong la cay diep ha chau. Dieu nay Iy giai tgi sao kha nang khang khuan cua dich chief vdi 2 loai dung mdi nay Iai tdt hon so vdi dung mdi nudc va clorofom.
Ddi vdi djch chief la cay hoin nggc, khi su dung dung mdi nudc, etiianol, metanola va clorofom diu cho k i t qua dudng kinh vdng khang vi khuin V.
vuIniScus va Streptococcus spp kem, dao ddng tir 4,75 mm d i n 9,04 mm. Hien nay, chua cd mgt nghien ciiu nao di sau v l tac dung phdng va t n benh ciia Ioai thio dugc nay frong nudi frong fhuy san ma chi ehii ylu tgp trung d i n chda trj cac bdnh frdn ngudi va mdt sd ddi tugng nudi khac. Nhu vay, vdi thu nghi|m ban diu cua chiing tdi ve kha nang khang khuan cua dich chilt la eay hoan nggc dd'i vdi vi khuan V.
vuIniScus va Streptococus spp thi thay r i n g hieu qua khang khuan kem.
Nhu vay, thir nghiem ban diu vdi 4 Ioai dung mdi khac nhau de chief xuat dieh chief cu tdi, li cay hoan ngpc va la eay diep ha chau thi chi cd ethanol va metanola cho hieu qua khang khuan cao hon so vdi nudc va clorofom. Theo Sukanya va cdng su, 2009 hiu h i t cac thanh phan cd boat tinh khang khuan frong cac loai thao dugc la nhiing hgp chat huu co thom hoac bao hda, dd tan frong ethanol va metanola. Do dd 2 loai dung mdi nay cd tic dung kich hoat thiosunfat va luu huynh tdng sd frong tdi va ancaloit frong la didp ha chau lam tang kha nSng khang khuan cua chiing. Rieng doi vdi la cay hoari nggc, ca 4 Ioai dung mdi nay ddu eho miie khang khuan kem ddi vdi vi khuan nghien ciiu.
3.2. Ndng dd uc c h i tdi thilu (MIC) ciia djch chilt thao dugc ddi vdi vl khuin V. vuIniScus vi Streptiicocus spp
Bang 2. Ndng dd tic chd ^ i thilu MIC cua dich chilt tiiaoduDC
m
Dd tiu 3. Anh hudng cua cac logi dung mdi d i n kha nang khing khuin ciia dich chilt la hoan nggc
Thao Auac
Cli toi {Allium sativum)
La cay di^p ha chau {Pbyllantiius
amarus)
Dung mdi
Nudc Ethanol Metanola Clorofom Nuoc Ethanol Metanola Clorofom
Nong do uc chi tdi thilu - MIC (pg/ml) V. vulniBcus
128 64 64 256 2048 2048 1024 4096
Stieptococcus spp 128 64 64 512 4096 1024 1024 4096
84 NONG NGHIEP VA PHAT TRIEN NONG THON - KY 1 - THANG 9/20U
KHOA HOC CbNG NGHfi
Tu k i t qua nghidn ctiu nOi dung 1, do dich chilt la cay hoin nggc khing khuan kem vdi vi khuin V.
vuhuScus va Streptococcus spp ndn chiing tdi chi tiln hanh thi nghidm xac djnh ndng dd tic c h i tdi thilu (MIC) cua djch chilt cii toi va la cay diep ha chau ddi vdi vi khuan nghidn ciiu.
Kit qui nghidn ciiu cho thiy djch chilt cu tdi va l i didp ha chau vdi cic loai dung mdi khac nhau cd boat tinh khang khuan khdng gidng nhau, tae ddng mgnh nhit la vdi dung mdi ethanol va methanola vdi ndng do lie chi vi khuin V. vuIniScus va Stieptococcus spp la 64 pg/ml, ke den la dung mdi nudc vdi MIC l i 128 pg/ml, dung mdi clorofom vdi MIC Ian lugt la 256 pg/ilil va 512 pg/ml.
Djch chilt la didp hg chau vdi dung mdi metanola cd hogt tinh khang khuin tnmg binh vdi MIC la 1024 pg/rtd, cae dung mdi cdn lai cd miic khang khuan ylu vdi MIC dao ddng hi 2048 pg/ml din 4096 pg/ml. Nghidn ciiu cua Hu^nh Kim Didu va Lf Thi Loan Em vd kha nang khang khuan cua djch dp la didp hg chau cho tiiiy nd tac dgng rit mgnh frln Edwardsiella tarda (MIC = 64-512 pg/mD, k l din Aeromonas hydrophUa (MIC=512-1024 pg/ml) va tae ddng ylu fren Streptococcus faecalis vk E. coh (MIC= 2048- 4096 pg/ml). Nhu vgy, kha nang tic dOng cfia djch chilt la didp hg chau ddi vdi vi khuan V. vubiiScus vk Streptococcus spp cung tuang duong vdi dgng dich dp vdi vi khuin Stieptococcus faecalis vk E. coli
4.K£rLUiN
Vdi dung mdi ethanol va metanola, djch chief cu tdi dgt tdt, dudng kinh vdng khang khuan 20,12 mm - 21,71 mm, djch chilt l i digp hg chau dgt trung bmh, dudng kinh vdng 14,61 mm -16,23 mm. Rieng ddi vdi djch chilt la hoan nggc frong tit ca eae loai dung mdi diu cd miic khang khuan kem, dirdng kinh vdng khang khuin 4,75 - 9,09 mm.
Ndng dg lie c h i tii thilu ddi vdi V. vuIniScus va Streptococcus spp cua djch chilt cu tdi khi sir dung dung mdi ethanol, metanola vdi MIC = 64 pg/ml, nudc vdi MIC = 128 pg/ml, clorofom vdi MIC lin lugt l i 256 pg/ml va 512 pg/ml. Djch chilt Ii didp hg chau vdi dung mdi metangia ed hogt tinh khang khuin trung binh vdi MIC Ii 1024 pg/ml, cic dung mdi cdn lgi cd miic khang khuin ylu vdi MIC dao ddng tir 2048 pg/ml din 4096 pg/ml.
TAIIfUTIMMIOlU
1. HuSmh Kim Dieu, Ly Thj Loan Em, 2011.
Dinh gia dac tinh thuin chiing va hoat tinh khang khuan ciia cay cd muc (Edipta prostrate) va eay didp ha chau than xanh (Phyllanthus niruri) b dong bang sdng Cuu Long. Tap chi Khoa hge 2011: 19^ 149 - 155. Tnidng Dgi hge Cin Tho.
2. Nguydn Thanh Hai, Bid Thj Tho, 2013.
Nghidn ciiu tac dung diet khuan in vitro cua djch chilt tdi {Alhum sativum L) ddi vdi E. coh'^y bdnh va E. cioA'khang amptxillin, kanamyxin. T E ^ chi Khoa bgc va Phat tiiln 2013, tap 11, sd 6:804 - 808.
3. Tu Minh Kodng va cdng su, 2001. Ky thugt san xuat dugc phim. Tap I. Trudng Dgi hge Dugc, HaNdi.
4. Dd Tit Lgi, 1968. Nhftng cay thudc va vi thudc Viet Nam. NXB Khoa hge va Ky tiiuat H i Ndi, fr. 446 -447.
5. Truong Cdng Quydn va edng su, 1986. Thtrc hanh dugc khoa. NXB Y hoc.
6. Nguydn Thj Van Thai, Nguydn Mmh Phue, Ngd Thi Kun, Nguydn Kim Dg va Luu Thi Dung, 2003. Ban ve tilm nang phdng va chiia trj bdnh nhidm khuan bing khang sinh thao mde frong nudi frdng thuy sin. Bao cio cac cdng trinh khoa hge.
Nha xuat ban Ndng nghidp. Ha Ndi, 2003.
7. Biii Thj Tho, Nguydn Thi Thanh Ha (2009).
Dugc hdu bgc Thii y. NXB Ndng nghidp, Ha Ndi, frang 77-81.
8. Chu Mgnh Thing, Ld Thj Hdng Thao, Dd Vilt Mmh, Nguydn Thanh Long, 2007. Tuyln tap edng trinh nghien ciiu KHCN chan nudi gia cim, an toan thuc pham va mdi trudng. NXB Ndng nghidp. Ha NOi 2007.
9. Tran Xuan Thuyit, 2003. Cay didp ha chau va bdnh gan. Tgp chi Sue khde va Ddi sdng (9):12.
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STUDY EFFECTS OF TYPES SOLVENTS KESISTANT vmrio vubMcusfiiD Streptococcus spp SOME OF EXTRACTS HERBAL
Tran Ngoc Hung, Tiuong Tlii Ttanh Wnh, Tran Tlii Lam Giang Summary
The study results showed that the sohrent afiects the activity of herbs. When used for extracting ethanol and methanol extracts are resistant to V. vuIniScus and Streptococcus spp bacteria better than water and chloroform. Ethanol and methanol solvent antibacterial garlic extract of achieving good, with inhibitory zone ranging from 20.12 to 21.71 mm, Phyllanthus amarus leaf extract lowered continent averaged is irom 14.61 mm to 16.23 mm. Particularly for Pseuderanthemum bracteatum leaf extracts in all the solvents have less antibacterial, with inhibitory zone from 4.75 to 9.09 mm. The minimum inhibitory concentrations of V.
vulnificus and Streptococcus spp extracts of garlic when using solvent ethanol, methanol with respectively MIC . 64 pg/ml, MIC - 128 (ig/ml, chloroform with MIC 256 ng/ml and 512 fig/m. Lower Phyllanthus amarus leaf extract solvent methanol average antibacterial activity with MIC is 1024 ^g/mi, the solvent renraining weak antibacterial MIC levels ranged from 2048 jig / ml to 4096 ng /ml.
Keyword.' Garlic, Phyllanthus amarus, Pseuderanthemum bracteatum, inhibitory zone, minimum inhibitory concentrations, attract
Ngu6i phin bien: TS. Hi Ky Ngiy nh^n bii: 25/4/2014 Ngiy thflng qua phin bien: 26/5/2014 Ngiy duyet dang: 2/6/2014
NONG NGHIEP VA PHAT TRIEN NONG THON - KY 1 - THANG 9/2014
